MD14G1237700.v1.1

Lipid transfer-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0004105
Physical Location & Seq
Reverse (-)
31720722 .. 31722029
1308 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1237700.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGCTTCGATCTCAAAACACTTGCTTCTCTTCTTGTTTCTGTCCCTTTTCGAGTTGTTTGGAGTCTCCCATGGCCAGAAGCTTACTGCATTGCCTGATAAGGAGGGCGCTGCTGGTGGCGGTAGTGATTCTTCCATGCAATGCGTGCAGAAACTGATTCCTTGTCAGGCGTATTTGAAGGCGCCGGAGAAGGCTCCCTCGATGTGCTGCTCGCCACTCAAGAAGTTGGTTTCGGATGATACCGAATGCCTTTGCACCCTCTTCAACAACGATGACATGTTGAGCAACTTTAACATAACGCAGGGTGATGCCTTGAAGCTGTCCAAGGCTTGTGATGCCAATGCTGACATTTCAGTATGCAAAAAGGATGCAGCATCACCAGCCAGTACAACATCACCTTCTAACAACAGTTCTTCCTCAGGCAACTCTCCCGGCAGTTCAAAGAGTGCAGCTTATGTGCTGTCCCCACTCGCCGTATCTGGTTTTGCTGCCGTCTTCATGGCTTTGATCATTGCAGCATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

175

Amino Acids

18.03

Weight (kDa)

6.05

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 44 - 120 6.6e-14 Probable lipid transfer
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AciI CCGC 1 cut(s) 120
AcoI YGGCCR 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 182
AfaI GTAC 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 276
AgsI TTSAA 4 cut(s) 178, 265, 316, 441
AluBI AGCT 3 cut(s) 82, 319, 452
AluI AGCT 3 cut(s) 82, 319, 452
Alw26I GTCTC 1 cut(s) 70
AlwNI CAGNNNCTG 1 cut(s) 154
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 6 cut(s) 110, 207, 371, 449, 488, 515
AspLEI GCGC 2 cut(s) 110, 184
AsuC2I CCSGG 1 cut(s) 432
AsuHPI GGTGA 3 cut(s) 317, 369, 387
AxyI CCTNAGG 1 cut(s) 418
BalI TGGCCA 1 cut(s) 75
BanI GGYRCC 1 cut(s) 181
BarI GAAGNNNNNNTAC 2 cut(s) 115, 147
BbsI GAAGAC 1 cut(s) 487
BbvI GCAGC 5 cut(s) 97, 194, 383, 461, 475
BceAI ACGGC 2 cut(s) 458, 476
BclI TGATCA 1 cut(s) 507
BcnI CCSGG 1 cut(s) 432
BcoDI GTCTC 1 cut(s) 70
BfoI RGCGCY 2 cut(s) 111, 185
BisI GCNGC 6 cut(s) 111, 208, 372, 450, 489, 516
BlsI GCNGC 6 cut(s) 112, 209, 373, 451, 490, 517
Bme1390I CCNGG 1 cut(s) 432
BmiI GGNNCC 2 cut(s) 183, 195
BmrFI CCNGG 1 cut(s) 432
BmsI GCATC 4 cut(s) 298, 325, 358, 383
BpiI GAAGAC 1 cut(s) 487
BpuEI CTTGAG 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 432
BsaHI GRCGYC 1 cut(s) 182
BsaJI CCNNGG 2 cut(s) 70, 324
Bse1I ACTGG 1 cut(s) 384
Bse21I CCTNAGG 1 cut(s) 418
Bse3DI GCAATG 3 cut(s) 89, 146, 510
BseDI CCNNGG 2 cut(s) 70, 324
BseGI GGATG 2 cut(s) 241, 373
BseMI GCAATG 3 cut(s) 89, 146, 510
BseMII CTCAG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 384
BseXI GCAGC 5 cut(s) 97, 194, 383, 461, 475
BsgI GTGCAG 2 cut(s) 167, 468
BshFI GGCC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 185, 432
BslFI GGGAC 2 cut(s) 28, 448
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 2 cut(s) 28, 448
BsmI GAATGC 1 cut(s) 251
BsnI GGCC 1 cut(s) 75
Bsp143I GATC 2 cut(s) 9, 507
Bsp19I CCATGG 1 cut(s) 70
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 1 cut(s) 431
BspLI GGNNCC 2 cut(s) 183, 195
BspT107I GGYRCC 1 cut(s) 181
BsrDI GCAATG 3 cut(s) 89, 146, 510
BsrI ACTGG 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 70, 324
BssMI GATC 2 cut(s) 9, 507
BssNI GRCGYC 1 cut(s) 182
BssT1I CCWWGG 2 cut(s) 70, 324
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 2 cut(s) 35, 266
BstACI GRCGYC 1 cut(s) 182
BstAPI GCANNNNNTGC 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 146, 212
BstDEI CTNAG 1 cut(s) 418
BstDSI CCRYGG 1 cut(s) 70
BstF5I GGATG 2 cut(s) 241, 373
BstH2I RGCGCY 2 cut(s) 111, 185
BstHHI GCGC 2 cut(s) 110, 184
BstKTI GATC 2 cut(s) 12, 510
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 2 cut(s) 9, 507
BstMWI GCNNNNNNNGC 3 cut(s) 145, 335, 380
BstNSI RCATGY 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 430
BstV1I GCAGC 5 cut(s) 97, 194, 383, 461, 475
BstV2I GAAGAC 1 cut(s) 487
Bsu36I CCTNAGG 1 cut(s) 418
BsuRI GGCC 1 cut(s) 75
BtgI CCRYGG 1 cut(s) 70
BtsCI GGATG 2 cut(s) 241, 373
Cac8I GCNNGC 2 cut(s) 146, 212
CaiI CAGNNNCTG 1 cut(s) 154
CfoI GCGC 2 cut(s) 110, 184
Csp6I GTAC 1 cut(s) 387
CviAII CATG 4 cut(s) 71, 136, 277, 499
CviJI RGCY 9 cut(s) 5, 75, 82, 194, 319, 329, 383, 452, 503
CviKI_1 RGCY 9 cut(s) 5, 75, 82, 194, 319, 329, 383, 452, 503
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 1 cut(s) 418
DinI GGCGCC 1 cut(s) 183
DpnI GATC 2 cut(s) 11, 509
DpnII GATC 2 cut(s) 9, 507
EaeI YGGCCR 1 cut(s) 73
Eam1104I CTCTTC 2 cut(s) 35, 266
EarI CTCTTC 2 cut(s) 35, 266
Eco130I CCWWGG 2 cut(s) 70, 324
Eco81I CCTNAGG 1 cut(s) 418
EcoT14I CCWWGG 2 cut(s) 70, 324
EgeI GGCGCC 1 cut(s) 183
EheI GGCGCC 1 cut(s) 183
ErhI CCWWGG 2 cut(s) 70, 324
FaeI CATG 4 cut(s) 74, 139, 280, 502
FaiI YATR 7 cut(s) 72, 137, 278, 296, 358, 456, 500
FaqI GGGAC 2 cut(s) 28, 448
FatI CATG 4 cut(s) 70, 135, 276, 498
FbaI TGATCA 1 cut(s) 507
Fnu4HI GCNGC 6 cut(s) 111, 208, 372, 450, 489, 516
FokI GGATG 2 cut(s) 248, 380
Fsp4HI GCNGC 6 cut(s) 111, 208, 372, 450, 489, 516
GlaI GCGC 2 cut(s) 109, 183
GluI GCNGC 6 cut(s) 111, 208, 372, 450, 489, 516
HaeII RGCGCY 2 cut(s) 111, 185
HaeIII GGCC 1 cut(s) 75
HapII CCGG 2 cut(s) 185, 432
HhaI GCGC 2 cut(s) 110, 184
Hin1I GRCGYC 1 cut(s) 182
Hin1II CATG 4 cut(s) 74, 139, 280, 502
Hin6I GCGC 2 cut(s) 108, 182
HinP1I GCGC 2 cut(s) 108, 182
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 3 cut(s) 63, 128, 157
HpaII CCGG 2 cut(s) 185, 432
HphI GGTGA 3 cut(s) 317, 369, 387
Hpy188I TCNGA 1 cut(s) 235
Hpy188III TCNNGA 1 cut(s) 220
HpyAV CCTTC 3 cut(s) 172, 184, 408
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 8 cut(s) 89, 139, 148, 255, 360, 371, 449, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 335, 380
HpyF3I CTNAG 1 cut(s) 418
Hsp92I GRCGYC 1 cut(s) 182
Hsp92II CATG 4 cut(s) 74, 139, 280, 502
HspAI GCGC 2 cut(s) 108, 182
KasI GGCGCC 1 cut(s) 181
Ksp22I TGATCA 1 cut(s) 507
Kzo9I GATC 2 cut(s) 9, 507
LmnI GCTCC 1 cut(s) 199
Lsp1109I GCAGC 5 cut(s) 97, 194, 383, 461, 475
LweI GCATC 4 cut(s) 298, 325, 358, 383
MalI GATC 2 cut(s) 11, 509
MboI GATC 2 cut(s) 9, 507
MboII GAAGA 5 cut(s) 22, 123, 253, 405, 487
MlsI TGGCCA 1 cut(s) 75
MluNI TGGCCA 1 cut(s) 75
Mly113I GGCGCC 1 cut(s) 182
MlyI GAGTC 1 cut(s) 72
MnlI CCTC 4 cut(s) 97, 208, 269, 427
Mox20I TGGCCA 1 cut(s) 75
MscI TGGCCA 1 cut(s) 75
MseI TTAA 1 cut(s) 291
Msp20I TGGCCA 1 cut(s) 75
MspI CCGG 2 cut(s) 185, 432
MspR9I CCNGG 1 cut(s) 432
Mva1269I GAATGC 1 cut(s) 251
MwoI GCNNNNNNNGC 3 cut(s) 145, 335, 380
NarI GGCGCC 1 cut(s) 182
NciI CCSGG 1 cut(s) 432
NcoI CCATGG 1 cut(s) 70
NdeII GATC 2 cut(s) 9, 507
NlaIII CATG 4 cut(s) 74, 139, 280, 502
NlaIV GGNNCC 2 cut(s) 183, 195
NspI RCATGY 1 cut(s) 280
PciI ACATGT 1 cut(s) 276
PctI GAATGC 1 cut(s) 251
PfeI GAWTC 2 cut(s) 128, 157
PkrI GCNGC 6 cut(s) 112, 209, 373, 451, 490, 517
PleI GAGTC 1 cut(s) 71
PluTI GGCGCC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 71
PscI ACATGT 1 cut(s) 276
PspN4I GGNNCC 2 cut(s) 183, 195
PstNI CAGNNNCTG 1 cut(s) 154
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SaqAI TTAA 1 cut(s) 291
SatI GCNGC 6 cut(s) 111, 208, 372, 450, 489, 516
Sau3AI GATC 2 cut(s) 9, 507
SchI GAGTC 1 cut(s) 72
ScrFI CCNGG 1 cut(s) 432
SetI ASST 4 cut(s) 84, 321, 400, 454
SfaNI GCATC 4 cut(s) 298, 325, 358, 383
SfoI GGCGCC 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
SsiI CCGC 1 cut(s) 120
SspDI GGCGCC 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 2 cut(s) 70, 324
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 3 cut(s) 8, 51, 200
TatI WGTACW 1 cut(s) 386
TfiI GAWTC 2 cut(s) 128, 157
Tru1I TTAA 1 cut(s) 291
Tru9I TTAA 1 cut(s) 291
TseI GCWGC 6 cut(s) 110, 207, 371, 449, 488, 515
TspDTI ATGAA 1 cut(s) 487
XceI RCATGY 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.