MD15G1287700.v1.1

Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005227
Physical Location & Seq
Reverse (-)
26441552 .. 26443018
1467 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1287700.v1.1.491

Sequence Viewer

Length: 690 bp
ATGGACACTGGAGCGGCGGGAGTGGGTCTCGCCGCAGCAGCCGCCGACGATCCGTTCAAGGGATCGCCGTTCGCAGCCGTGTCGGATTGGAGTTACGGCGTTTCGCGGCGCTACCAGCACGTGCTCGACCGCACGACGCCGCACGTGCTGTACCGGTGGCTGGCGTGCCTGGCGGCGGCCTTAATCTACGTCTTACGCGTGTACCTGGTCCAAGGCTTCTACGTGGTGTCGTACGGCTTGGGGATCTACATTCTCAACCTCCTCATCGGGTTTCTCTCTCCTCAGGTCGACCCGGAGATCCACGACCTCTCCTCCTCCGACGGCCCCTCCCTCCCAACCCGCGGATCCGATGAGTTCCGCCCCTTCGTCCGCCGCCTCCCCGAGTTCAAGTTCTGGTACTCAATCACCAAGGCATTTTGCATCGCTTTTGCGATGACATTCTTCAGTGCGTTTGATGTACCTGTGTTCTGGCCAATACTTCTCTTCTACTGGGTGGTGCTCTTCGTTCTCACCATGAGGAGACAGATTGTACACATGATCAAATACAAATACGTCCCCTTCTCTTTTGGGAAACAGCGGTACGATGGAAAGAAAGCTCATTCGTCTTCACCACGGGAGTTTTTTTGGAAGATATGCGGCCTGGAGGAATCATCTTTGGAGCCAATTCACGCGTCTTGTATCTGGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

230

Amino Acids

26.16

Weight (kDa)

8.83

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rer1 PF03248 34 - 194 3.4e-78 Rer1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 14
AccI GTMKAC 1 cut(s) 288
AccII CGCG 4 cut(s) 106, 198, 342, 671
AclWI GGATC 6 cut(s) 44, 70, 251, 292, 339, 352
AcoI YGGCCR 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 427
AcvI CACGTG 2 cut(s) 121, 145
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 7 cut(s) 152, 203, 233, 398, 459, 531, 581
AfiI CCNNNNNNNGG 4 cut(s) 59, 160, 175, 341
AflIII ACRYGT 2 cut(s) 196, 669
AgeI ACCGGT 1 cut(s) 153
AgsI TTSAA 2 cut(s) 58, 388
AjnI CCWGG 3 cut(s) 168, 204, 639
AluBI AGCT 1 cut(s) 596
AluI AGCT 1 cut(s) 596
Alw21I GWGCWC 2 cut(s) 126, 501
Alw26I GTCTC 2 cut(s) 32, 514
AlwI GGATC 6 cut(s) 44, 70, 251, 292, 339, 352
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 4 cut(s) 177, 322, 470, 637
ApeKI GCWGC 3 cut(s) 35, 38, 74
AsiGI ACCGGT 1 cut(s) 153
AspLEI GCGC 1 cut(s) 111
AspS9I GGNCC 2 cut(s) 208, 323
AsuC2I CCSGG 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 397, 502, 600
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 1 cut(s) 208
AxyI CCTNAGG 1 cut(s) 282
BaeI ACNNNNGTAYC 2 cut(s) 185, 218
BalI TGGCCA 1 cut(s) 472
BamHI GGATCC 1 cut(s) 344
BbrPI CACGTG 2 cut(s) 121, 145
BbsI GAAGAC 1 cut(s) 597
Bbv12I GWGCWC 2 cut(s) 126, 501
BbvI GCAGC 3 cut(s) 47, 50, 86
BccI CCATC 1 cut(s) 578
BceAI ACGGC 5 cut(s) 52, 62, 112, 250, 337
BcgI CGANNNNNNTGC 2 cut(s) 63, 97
BciT130I CCWGG 3 cut(s) 170, 206, 641
BclI TGATCA 1 cut(s) 537
BcnI CCSGG 1 cut(s) 293
BcoDI GTCTC 2 cut(s) 32, 514
BfoI RGCGCY 1 cut(s) 112
Bme1390I CCNGG 4 cut(s) 170, 206, 293, 641
Bme18I GGWCC 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 2 cut(s) 208, 323
BmiI GGNNCC 3 cut(s) 325, 346, 660
BmrFI CCNGG 4 cut(s) 170, 206, 293, 641
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 429
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 1 cut(s) 597
BplI GAGNNNNNCTC 2 cut(s) 12, 44
BpmI CTGGAG 2 cut(s) 30, 662
BpuMI CCSGG 1 cut(s) 293
BsaAI YACGTR 3 cut(s) 121, 145, 223
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 4 cut(s) 211, 340, 408, 611
BsaWI WCCGGW 1 cut(s) 153
BsaXI ACNNNNNCTCC 6 cut(s) 293, 296, 311, 323, 326, 341
Bsc4I CCNNNNNNNGG 4 cut(s) 59, 160, 175, 341
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 2 cut(s) 13, 494
Bse21I CCTNAGG 1 cut(s) 282
BseBI CCWGG 3 cut(s) 170, 206, 641
BseDI CCNNGG 4 cut(s) 211, 340, 408, 611
BseLI CCNNNNNNNGG 4 cut(s) 59, 160, 175, 341
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 13, 494
BseRI GAGGAG 5 cut(s) 251, 270, 301, 304, 532
BseXI GCAGC 3 cut(s) 47, 50, 86
Bsh1236I CGCG 4 cut(s) 106, 198, 342, 671
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 4 cut(s) 179, 324, 472, 639
BshTI ACCGGT 1 cut(s) 153
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 2 cut(s) 126, 501
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 2 cut(s) 154, 293
BsiWI CGTACG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 539
BslI CCNNNNNNNGG 4 cut(s) 59, 160, 175, 341
BsmAI GTCTC 2 cut(s) 32, 514
BsmFI GGGAC 1 cut(s) 539
BsnI GGCC 4 cut(s) 179, 324, 472, 639
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 126, 501
Bsp1407I TGTACA 1 cut(s) 529
Bsp143I GATC 6 cut(s) 49, 62, 243, 297, 344, 537
BspANI GGCC 4 cut(s) 179, 324, 472, 639
BspCNI CTCAG 1 cut(s) 295
BspFNI CGCG 4 cut(s) 106, 198, 342, 671
BspLI GGNNCC 3 cut(s) 325, 346, 660
BspPI GGATC 6 cut(s) 44, 70, 251, 292, 339, 352
BspQI GCTCTTC 1 cut(s) 506
BspTNI GGTCTC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 14
BsrFI RCCGGY 1 cut(s) 153
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 2 cut(s) 13, 494
BssAI RCCGGY 1 cut(s) 153
BssECI CCNNGG 4 cut(s) 211, 340, 408, 611
BssMI GATC 6 cut(s) 49, 62, 243, 297, 344, 537
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 2 cut(s) 211, 408
Bst2UI CCWGG 3 cut(s) 170, 206, 641
Bst6I CTCTTC 2 cut(s) 488, 506
BstACI GRCGYC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 529
BstBAI YACGTR 3 cut(s) 121, 145, 223
BstC8I GCNNGC 2 cut(s) 162, 166
BstDEI CTNAG 1 cut(s) 282
BstDSI CCRYGG 2 cut(s) 340, 611
BstFNI CGCG 4 cut(s) 106, 198, 342, 671
BstH2I RGCGCY 1 cut(s) 112
BstHHI GCGC 1 cut(s) 111
BstKTI GATC 6 cut(s) 52, 65, 246, 300, 347, 540
BstMAI GTCTC 2 cut(s) 32, 514
BstMBI GATC 6 cut(s) 49, 62, 243, 297, 344, 537
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 5 cut(s) 38, 41, 115, 145, 170
BstNI CCWGG 3 cut(s) 170, 206, 641
BstSCI CCNGG 4 cut(s) 168, 204, 291, 639
BstUI CGCG 4 cut(s) 106, 198, 342, 671
BstV1I GCAGC 3 cut(s) 47, 50, 86
BstV2I GAAGAC 1 cut(s) 597
BstX2I RGATCY 3 cut(s) 243, 297, 344
BstYI RGATCY 3 cut(s) 243, 297, 344
Bsu36I CCTNAGG 1 cut(s) 282
BsuRI GGCC 4 cut(s) 179, 324, 472, 639
BtgI CCRYGG 2 cut(s) 340, 611
BtgZI GCGATG 2 cut(s) 406, 446
BtsIMutI CAGTG 2 cut(s) 6, 451
Cac8I GCNNGC 2 cut(s) 162, 166
CfoI GCGC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 2 cut(s) 208, 323
Cfr42I CCGCGG 1 cut(s) 343
CseI GACGC 2 cut(s) 145, 660
CsiI ACCWGGT 1 cut(s) 204
Csp6I GTAC 7 cut(s) 151, 202, 232, 397, 458, 530, 580
CspAI ACCGGT 1 cut(s) 153
CviAII CATG 2 cut(s) 514, 535
CviQI GTAC 7 cut(s) 151, 202, 232, 397, 458, 530, 580
DdeI CTNAG 1 cut(s) 282
DpnI GATC 6 cut(s) 51, 64, 245, 299, 346, 539
DpnII GATC 6 cut(s) 49, 62, 243, 297, 344, 537
EaeI YGGCCR 1 cut(s) 470
Eam1104I CTCTTC 2 cut(s) 488, 506
EarI CTCTTC 2 cut(s) 488, 506
EciI GGCGGA 2 cut(s) 347, 359
Eco130I CCWWGG 2 cut(s) 211, 408
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 427
Eco72I CACGTG 2 cut(s) 121, 145
Eco81I CCTNAGG 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 380
EcoRII CCWGG 3 cut(s) 168, 204, 639
EcoT14I CCWWGG 2 cut(s) 211, 408
ErhI CCWWGG 2 cut(s) 211, 408
FaeI CATG 2 cut(s) 517, 538
FaiI YATR 4 cut(s) 515, 536, 634, 688
FaqI GGGAC 1 cut(s) 539
FatI CATG 2 cut(s) 513, 534
FauI CCCGC 2 cut(s) 10, 347
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 1 cut(s) 288
GlaI GCGC 1 cut(s) 110
GsuI CTGGAG 2 cut(s) 30, 662
HaeII RGCGCY 1 cut(s) 112
HaeIII GGCC 4 cut(s) 179, 324, 472, 639
HapII CCGG 2 cut(s) 154, 293
HgaI GACGC 2 cut(s) 145, 660
HhaI GCGC 1 cut(s) 111
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 2 cut(s) 517, 538
Hin6I GCGC 1 cut(s) 109
HinP1I GCGC 1 cut(s) 109
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HinfI GANTC 1 cut(s) 647
HpaII CCGG 2 cut(s) 154, 293
HphI GGTGA 3 cut(s) 397, 502, 600
Hpy166II GTNNAC 3 cut(s) 202, 289, 532
Hpy188I TCNGA 3 cut(s) 85, 319, 349
Hpy188III TCNNGA 1 cut(s) 682
Hpy8I GTNNAC 3 cut(s) 202, 289, 532
Hpy99I CGWCG 3 cut(s) 50, 139, 323
HpyAV CCTTC 2 cut(s) 373, 568
HpyCH4IV ACGT 5 cut(s) 120, 144, 189, 222, 552
HpyCH4V TGCA 1 cut(s) 420
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 41, 115, 145, 170
HpyF3I CTNAG 1 cut(s) 282
HpySE526I ACGT 5 cut(s) 120, 144, 189, 222, 552
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 2 cut(s) 517, 538
HspAI GCGC 1 cut(s) 109
Ksp22I TGATCA 1 cut(s) 537
KspI CCGCGG 1 cut(s) 343
Kzo9I GATC 6 cut(s) 49, 62, 243, 297, 344, 537
LguI GCTCTTC 1 cut(s) 506
LmnI GCTCC 2 cut(s) 11, 658
Lsp1109I GCAGC 3 cut(s) 47, 50, 86
LweI GCATC 1 cut(s) 429
MabI ACCWGGT 1 cut(s) 204
MaeII ACGT 5 cut(s) 120, 144, 189, 222, 552
MaeIII GTNAC 1 cut(s) 92
MalI GATC 6 cut(s) 51, 64, 245, 299, 346, 539
MbiI CCGCTC 1 cut(s) 14
MboI GATC 6 cut(s) 49, 62, 243, 297, 344, 537
MboII GAAGA 5 cut(s) 433, 475, 493, 597, 640
MflI RGATCY 3 cut(s) 243, 297, 344
MhlI GDGCHC 2 cut(s) 126, 501
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 1 cut(s) 663
MluI ACGCGT 2 cut(s) 196, 669
MluNI TGGCCA 1 cut(s) 472
MmeI TCCRAC 2 cut(s) 63, 342
Mox20I TGGCCA 1 cut(s) 472
MscI TGGCCA 1 cut(s) 472
MseI TTAA 1 cut(s) 182
Msp20I TGGCCA 1 cut(s) 472
MspA1I CMGCKG 2 cut(s) 342, 577
MspI CCGG 2 cut(s) 154, 293
MspR9I CCNGG 4 cut(s) 170, 206, 293, 641
MvaI CCWGG 3 cut(s) 170, 206, 641
MvnI CGCG 4 cut(s) 106, 198, 342, 671
MwoI GCNNNNNNNGC 5 cut(s) 38, 41, 115, 145, 170
NciI CCSGG 1 cut(s) 293
NdeII GATC 6 cut(s) 49, 62, 243, 297, 344, 537
NlaIII CATG 2 cut(s) 517, 538
NlaIV GGNNCC 3 cut(s) 325, 346, 660
PciSI GCTCTTC 1 cut(s) 506
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PfeI GAWTC 1 cut(s) 647
Pfl23II CGTACG 1 cut(s) 231
PinAI ACCGGT 1 cut(s) 153
PmaCI CACGTG 2 cut(s) 121, 145
PmlI CACGTG 2 cut(s) 121, 145
Ppu21I YACGTR 3 cut(s) 121, 145, 223
Psp6I CCWGG 3 cut(s) 168, 204, 639
PspCI CACGTG 2 cut(s) 121, 145
PspGI CCWGG 3 cut(s) 168, 204, 639
PspLI CGTACG 1 cut(s) 231
PspN4I GGNNCC 3 cut(s) 325, 346, 660
PspPI GGNCC 2 cut(s) 208, 323
PsuI RGATCY 3 cut(s) 243, 297, 344
RsaI GTAC 7 cut(s) 152, 203, 233, 398, 459, 531, 581
RsaNI GTAC 7 cut(s) 151, 202, 232, 397, 458, 530, 580
SacII CCGCGG 1 cut(s) 343
SalI GTCGAC 1 cut(s) 287
SapI GCTCTTC 1 cut(s) 506
SaqAI TTAA 1 cut(s) 182
Sau3AI GATC 6 cut(s) 49, 62, 243, 297, 344, 537
Sau96I GGNCC 2 cut(s) 208, 323
ScrFI CCNGG 4 cut(s) 170, 206, 293, 641
SduI GDGCHC 2 cut(s) 126, 501
SexAI ACCWGGT 1 cut(s) 204
SfaNI GCATC 1 cut(s) 429
Sfr303I CCGCGG 1 cut(s) 343
SgrBI CCGCGG 1 cut(s) 343
SinI GGWCC 1 cut(s) 208
Sse9I AATT 1 cut(s) 663
StyD4I CCNGG 4 cut(s) 168, 204, 291, 639
StyI CCWWGG 2 cut(s) 211, 408
TaiI ACGT 5 cut(s) 123, 147, 192, 225, 555
TaqI TCGA 2 cut(s) 126, 288
TasI AATT 1 cut(s) 663
TatI WGTACW 1 cut(s) 529
TauI GCSGC 9 cut(s) 17, 35, 44, 109, 142, 176, 179, 375, 639
TfiI GAWTC 1 cut(s) 647
Tru1I TTAA 1 cut(s) 182
Tru9I TTAA 1 cut(s) 182
TscAI CASTG 2 cut(s) 13, 451
TseI GCWGC 3 cut(s) 35, 38, 74
TspGWI ACGGA 1 cut(s) 42
TspRI CASTG 2 cut(s) 13, 451
VpaK11BI GGWCC 1 cut(s) 208
XmiI GTMKAC 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.