Rorug02G0135500

Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0005227
Physical Location & Seq
Reverse (-)
11704050 .. 11706072
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0135500.1

Sequence Viewer

Length: 687 bp
ATGGATCCAGATGCAGTCAAATCCACTCTCCAAAACCTAGCATTTGGAAATGTAATGGCCGCTGCTGCCCGCAATTACCAGAAGGAAATTATCGCCGAAGGAAAAGCTCCGGCCACCAGCTCCGCCGAGCAGGAGGTCGACCTTGACGACCTCATGGATGATCCTGAGCTGGAAAAATTGCACGCGGATCGAATTGCAGCTCTCAAGAGAGAAGCTGAGAAGAGGGAAGCTTTGAAGAGGCAAGGGCATGGAGAATATAGGGAAATTACTGAGGGGGATTTCTTAGGTGAAGTTACTGGGAGCGAGAAGGCCATCTGCCACTTTTATCATAGAGAATTCTATAGATGCAAGATAATGGACAAGCATTTGAAGACCCTTTCTTTAAAGCATGTTGATACCAAGTTCATCAAACTGGATGCCGAGAATGCGCCCTTCTTCGTCACCAAGCTAGGAGTCAAAACTCTGCCTTGTGTCATAATATTCAGAAAAGGAGTTGCTGTGGATAGGCTGATTGGATTTATTGATATGGGTGGAAAAGATGATTTCAGCACAAGGGCACTTGAGGTTGTCCTAATCAAGAAAGGTATAATTAGTGAGAAGAAAGAAGAAGACGAAGATGATGATTATCTTGAAGGTGGTCGCAGGACAGTGAGATCATCTGTGAATCATGATTCTGATTCAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

228

Amino Acids

25.66

Weight (kDa)

5.38

Isoelectric Point (pI)

29.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 185
AciI CCGC 4 cut(s) 60, 70, 123, 185
AclWI GGATC 3 cut(s) 12, 155, 195
AcoI YGGCCR 2 cut(s) 57, 111
AcsI RAATTY 1 cut(s) 335
AgsI TTSAA 3 cut(s) 235, 370, 632
AluBI AGCT 7 cut(s) 107, 120, 169, 200, 215, 230, 448
AluI AGCT 7 cut(s) 107, 120, 169, 200, 215, 230, 448
AlwI GGATC 3 cut(s) 12, 155, 195
AoxI GGCC 3 cut(s) 57, 111, 309
ApeKI GCWGC 3 cut(s) 62, 65, 197
ApoI RAATTY 1 cut(s) 335
AspLEI GCGC 1 cut(s) 430
AsuHPI GGTGA 2 cut(s) 299, 433
BaeGI GKGCMC 1 cut(s) 559
BamHI GGATCC 1 cut(s) 4
BbsI GAAGAC 2 cut(s) 377, 615
BbvI GCAGC 3 cut(s) 49, 52, 209
BccI CCATC 1 cut(s) 320
BcgI CGANNNNNNTGC 2 cut(s) 170, 204
BfaI CTAG 2 cut(s) 38, 449
BfmI CTRYAG 1 cut(s) 340
BisI GCNGC 4 cut(s) 60, 63, 66, 198
BlsI GCNGC 4 cut(s) 61, 64, 67, 199
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 2 cut(s) 335, 406
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 2 cut(s) 377, 615
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 2 cut(s) 188, 581
BsaBI GATNNNNATC 1 cut(s) 624
BsaXI ACNNNNNCTCC 2 cut(s) 483, 513
Bse1I ACTGG 2 cut(s) 301, 417
Bse8I GATNNNNATC 1 cut(s) 624
BseGI GGATG 2 cut(s) 163, 421
BseJI GATNNNNATC 1 cut(s) 624
BseMII CTCAG 3 cut(s) 156, 207, 261
BseNI ACTGG 2 cut(s) 301, 417
BseSI GKGCMC 1 cut(s) 559
BseXI GCAGC 3 cut(s) 49, 52, 209
Bsh1236I CGCG 1 cut(s) 185
BshFI GGCC 3 cut(s) 59, 113, 311
BsiSI CCGG 1 cut(s) 110
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 3 cut(s) 59, 113, 311
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 4 cut(s) 4, 160, 187, 653
BspACI CCGC 4 cut(s) 60, 70, 123, 185
BspANI GGCC 3 cut(s) 59, 113, 311
BspCNI CTCAG 3 cut(s) 157, 208, 262
BspFNI CGCG 1 cut(s) 185
BspHI TCATGA 1 cut(s) 667
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 3 cut(s) 12, 155, 195
BsrI ACTGG 2 cut(s) 301, 417
BssMI GATC 4 cut(s) 4, 160, 187, 653
Bst4CI ACNGT 1 cut(s) 649
Bst6I CTCTTC 2 cut(s) 215, 230
BstC8I GCNNGC 2 cut(s) 70, 183
BstDEI CTNAG 4 cut(s) 165, 216, 270, 283
BstF5I GGATG 2 cut(s) 163, 421
BstFNI CGCG 1 cut(s) 185
BstHHI GCGC 1 cut(s) 430
BstKTI GATC 4 cut(s) 7, 163, 190, 656
BstMBI GATC 4 cut(s) 4, 160, 187, 653
BstMWI GCNNNNNNNGC 2 cut(s) 65, 425
BstNSI RCATGY 1 cut(s) 392
BstSFI CTRYAG 1 cut(s) 340
BstSLI GKGCMC 1 cut(s) 559
BstUI CGCG 1 cut(s) 185
BstV1I GCAGC 3 cut(s) 49, 52, 209
BstV2I GAAGAC 2 cut(s) 377, 615
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 3 cut(s) 59, 113, 311
BtsCI GGATG 2 cut(s) 163, 421
BtsIMutI CAGTG 1 cut(s) 654
Cac8I GCNNGC 2 cut(s) 70, 183
CciI TCATGA 1 cut(s) 667
CfoI GCGC 1 cut(s) 430
CviAII CATG 4 cut(s) 154, 248, 389, 668
DdeI CTNAG 4 cut(s) 165, 216, 270, 283
DpnI GATC 4 cut(s) 6, 162, 189, 655
DpnII GATC 4 cut(s) 4, 160, 187, 653
DraI TTTAAA 1 cut(s) 384
EaeI YGGCCR 2 cut(s) 57, 111
Eam1104I CTCTTC 2 cut(s) 215, 230
EarI CTCTTC 2 cut(s) 215, 230
EciI GGCGGA 1 cut(s) 112
EcoRI GAATTC 1 cut(s) 335
FaeI CATG 4 cut(s) 157, 251, 392, 671
FatI CATG 4 cut(s) 153, 247, 388, 667
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 4 cut(s) 60, 63, 66, 198
FokI GGATG 2 cut(s) 170, 428
Fsp4HI GCNGC 4 cut(s) 60, 63, 66, 198
FspBI CTAG 2 cut(s) 38, 449
GlaI GCGC 1 cut(s) 429
GluI GCNGC 4 cut(s) 60, 63, 66, 198
HaeIII GGCC 3 cut(s) 59, 113, 311
HapII CCGG 1 cut(s) 110
HhaI GCGC 1 cut(s) 430
Hin1II CATG 4 cut(s) 157, 251, 392, 671
Hin6I GCGC 1 cut(s) 428
HinP1I GCGC 1 cut(s) 428
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 228
HinfI GANTC 4 cut(s) 453, 664, 671, 677
HpaII CCGG 1 cut(s) 110
HphI GGTGA 2 cut(s) 299, 433
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 3 cut(s) 485, 676, 682
Hpy188III TCNNGA 6 cut(s) 8, 164, 205, 577, 629, 668
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 5 cut(s) 76, 92, 301, 442, 626
HpyCH4III ACNGT 1 cut(s) 649
HpyCH4V TGCA 4 cut(s) 14, 181, 197, 348
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 425
HpyF3I CTNAG 4 cut(s) 165, 216, 270, 283
Hsp92II CATG 4 cut(s) 157, 251, 392, 671
HspAI GCGC 1 cut(s) 428
Kzo9I GATC 4 cut(s) 4, 160, 187, 653
LmnI GCTCC 3 cut(s) 112, 125, 300
Lsp1109I GCAGC 3 cut(s) 49, 52, 209
LweI GCATC 2 cut(s) 335, 406
MaeI CTAG 2 cut(s) 38, 449
MaeIII GTNAC 2 cut(s) 292, 439
MalI GATC 4 cut(s) 6, 162, 189, 655
MboI GATC 4 cut(s) 4, 160, 187, 653
MboII GAAGA 8 cut(s) 232, 247, 382, 427, 610, 617, 620, 626
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 7 cut(s) 73, 87, 176, 192, 264, 335, 588
MlyI GAGTC 1 cut(s) 462
MnlI CCTC 6 cut(s) 127, 161, 216, 231, 265, 556
MseI TTAA 1 cut(s) 383
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 430
MvnI CGCG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 65, 425
NdeII GATC 4 cut(s) 4, 160, 187, 653
NlaIII CATG 4 cut(s) 157, 251, 392, 671
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 2 cut(s) 151, 445
NmuCI GTSAC 1 cut(s) 439
NspI RCATGY 1 cut(s) 392
PagI TCATGA 1 cut(s) 667
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 3 cut(s) 664, 671, 677
PkrI GCNGC 4 cut(s) 61, 64, 67, 199
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 4
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 1 cut(s) 383
SatI GCNGC 4 cut(s) 60, 63, 66, 198
Sau3AI GATC 4 cut(s) 4, 160, 187, 653
SchI GAGTC 1 cut(s) 462
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 2 cut(s) 335, 406
SfcI CTRYAG 1 cut(s) 340
SmlI CTYRAG 2 cut(s) 203, 560
SmoI CTYRAG 2 cut(s) 203, 560
Sse9I AATT 7 cut(s) 73, 87, 176, 192, 264, 335, 588
SsiI CCGC 4 cut(s) 60, 70, 123, 185
SspI AATATT 1 cut(s) 480
SspMI CTAG 2 cut(s) 38, 449
TaaI ACNGT 1 cut(s) 649
TaqI TCGA 2 cut(s) 138, 190
TasI AATT 7 cut(s) 73, 87, 176, 192, 264, 335, 588
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 3 cut(s) 664, 671, 677
Tru1I TTAA 1 cut(s) 383
Tru9I TTAA 1 cut(s) 383
TscAI CASTG 1 cut(s) 654
TseFI GTSAC 1 cut(s) 439
TseI GCWGC 3 cut(s) 62, 65, 197
Tsp45I GTSAC 1 cut(s) 439
TspDTI ATGAA 1 cut(s) 394
TspRI CASTG 1 cut(s) 654
XapI RAATTY 1 cut(s) 335
XceI RCATGY 1 cut(s) 392
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 2 cut(s) 38, 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.