Rw2G014570

Involved in the retrieval of endoplasmic reticulum membrane proteins from the early Golgi compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005227
Physical Location & Seq
Reverse (-)
16412065 .. 16426162
14098 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014570.1

Sequence Viewer

Length: 618 bp
ATGGACACCGGAGCGGCGGGCGTGGGTCTCGCCGCGTCGTCGTCGTCGGCGTCGGCGGAGGACCCGTCGTCTCGGGGATCGCCGTTCGACGCTGTCCAGGCGTGGAGCTTCGGCGTGTCGCGCCGATACCAGCACCTGCTCGACAAAACGACGCCGCACGTCCTCCACCGCTGGCTCGCAACCCTCGGCGTGGCCTTGGTCTACGTCCTCCGCGTCTACTTCGTCCAGGGATTCTACGTCGTGTCGTACGGCTTGGGGATCTACCTCCTCAACTTGCTCATCGGCTTTCTCTCTCCTCAGGTCGACCCGGAGATCCACGACCTTTCCGACGGCCCCGGCCTCCCCACCCGCGGATCCGACGAGTTCCGCCCCTTCGTTCGACGCCTCCCCGAGTTTAAATTCTGGTACTCAATTACAAAGGCGTTTTGCATTGCTTTCGTGATGACATTCTTCAGTGCATTTGATGTGCCTGTGTTCTGGCCCATTCTTCTCTTCTACTGGCTAGTGCTCTTCGTTCTCACAATGAGGAGGCAGATCGTACACATGATCAAGTACAAATATGTTCCTTTCTCTATTGGCAAGCAGAGGTATGATGGAAAGAAAGCTTCTGCATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006810 GO:0006890 GO:0008150 GO:0016192 GO:0048193 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rer1

Protein Analysis

205

Amino Acids

23.22

Weight (kDa)

9.46

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rer1 PF03248 39 - 197 5e-78 Rer1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 144
Acc36I ACCTGC 1 cut(s) 144
AccBSI CCGCTC 1 cut(s) 14
AccI GTMKAC 3 cut(s) 201, 216, 303
AccII CGCG 4 cut(s) 35, 121, 213, 351
AclWI GGATC 5 cut(s) 85, 266, 307, 348, 361
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 436
AcyI GRCGYC 3 cut(s) 50, 152, 382
AfaI GTAC 4 cut(s) 248, 407, 540, 554
AfiI CCNNNNNNNGG 2 cut(s) 190, 350
AhdI GACNNNNNGTC 1 cut(s) 67
AjiI CACGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 96, 225
AluBI AGCT 2 cut(s) 108, 605
AluI AGCT 2 cut(s) 108, 605
Alw21I GWGCWC 1 cut(s) 510
Alw26I GTCTC 2 cut(s) 32, 75
AlwI GGATC 5 cut(s) 85, 266, 307, 348, 361
AlwNI CAGNNNCTG 1 cut(s) 136
Ama87I CYCGRG 2 cut(s) 72, 389
AoxI GGCC 4 cut(s) 192, 331, 337, 479
ApoI RAATTY 1 cut(s) 398
AspLEI GCGC 1 cut(s) 123
AspS9I GGNCC 3 cut(s) 61, 332, 480
AsuC2I CCSGG 2 cut(s) 308, 336
AvaI CYCGRG 2 cut(s) 72, 389
AvaII GGWCC 1 cut(s) 61
AxyI CCTNAGG 1 cut(s) 297
BamHI GGATCC 1 cut(s) 353
Bbv12I GWGCWC 1 cut(s) 510
BccI CCATC 1 cut(s) 587
BceAI ACGGC 3 cut(s) 67, 265, 346
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 2 cut(s) 98, 227
BclI TGATCA 1 cut(s) 546
BcnI CCSGG 2 cut(s) 308, 336
BcoDI GTCTC 2 cut(s) 32, 75
BfaI CTAG 1 cut(s) 503
BfuAI ACCTGC 1 cut(s) 144
BisI GCNGC 3 cut(s) 15, 33, 155
BlsI GCNGC 3 cut(s) 16, 34, 156
Bme1390I CCNGG 4 cut(s) 98, 227, 308, 336
Bme18I GGWCC 1 cut(s) 61
BmeRI GACNNNNNGTC 1 cut(s) 67
BmeT110I CYCGRG 2 cut(s) 72, 389
BmgBI CACGTC 1 cut(s) 160
BmgT120I GGNCC 3 cut(s) 61, 332, 480
BmiI GGNNCC 3 cut(s) 63, 334, 355
BmrFI CCNGG 4 cut(s) 98, 227, 308, 336
BpuMI CCSGG 2 cut(s) 308, 336
BsaHI GRCGYC 3 cut(s) 50, 152, 382
BsaI GGTCTC 1 cut(s) 32
BsaJI CCNNGG 5 cut(s) 184, 195, 226, 334, 349
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 350
Bse1I ACTGG 1 cut(s) 503
Bse21I CCTNAGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 429
BseBI CCWGG 2 cut(s) 98, 227
BseDI CCNNGG 5 cut(s) 184, 195, 226, 334, 349
BseLI CCNNNNNNNGG 2 cut(s) 190, 350
BseMI GCAATG 1 cut(s) 429
BseMII CTCAG 1 cut(s) 311
BseNI ACTGG 1 cut(s) 503
BseRI GAGGAG 3 cut(s) 257, 285, 541
Bsh1236I CGCG 4 cut(s) 35, 121, 213, 351
BshFI GGCC 4 cut(s) 194, 333, 339, 481
BsiHKAI GWGCWC 1 cut(s) 510
BsiHKCI CYCGRG 2 cut(s) 72, 389
BsiSI CCGG 3 cut(s) 9, 308, 336
BsiWI CGTACG 1 cut(s) 246
BslI CCNNNNNNNGG 2 cut(s) 190, 350
BsmAI GTCTC 2 cut(s) 32, 75
BsmBI CGTCTC 1 cut(s) 75
BsnI GGCC 4 cut(s) 194, 333, 339, 481
Bso31I GGTCTC 1 cut(s) 32
BsoBI CYCGRG 2 cut(s) 72, 389
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 6 cut(s) 77, 258, 312, 353, 534, 546
BspANI GGCC 4 cut(s) 194, 333, 339, 481
BspCNI CTCAG 1 cut(s) 310
BspFNI CGCG 4 cut(s) 35, 121, 213, 351
BspLI GGNNCC 3 cut(s) 63, 334, 355
BspMI ACCTGC 1 cut(s) 144
BspPI GGATC 5 cut(s) 85, 266, 307, 348, 361
BspQI GCTCTTC 1 cut(s) 515
BspTNI GGTCTC 1 cut(s) 32
BsrBI CCGCTC 1 cut(s) 14
BsrDI GCAATG 1 cut(s) 429
BsrI ACTGG 1 cut(s) 503
BssECI CCNNGG 5 cut(s) 184, 195, 226, 334, 349
BssMI GATC 6 cut(s) 77, 258, 312, 353, 534, 546
BssNI GRCGYC 3 cut(s) 50, 152, 382
BssT1I CCWWGG 1 cut(s) 195
Bst2UI CCWGG 2 cut(s) 98, 227
Bst6I CTCTTC 2 cut(s) 497, 515
BstACI GRCGYC 3 cut(s) 50, 152, 382
BstC8I GCNNGC 4 cut(s) 19, 173, 177, 581
BstDEI CTNAG 1 cut(s) 297
BstDSI CCRYGG 1 cut(s) 349
BstFNI CGCG 4 cut(s) 35, 121, 213, 351
BstHHI GCGC 1 cut(s) 123
BstKTI GATC 6 cut(s) 80, 261, 315, 356, 537, 549
BstMAI GTCTC 2 cut(s) 32, 75
BstMBI GATC 6 cut(s) 77, 258, 312, 353, 534, 546
BstMWI GCNNNNNNNGC 2 cut(s) 98, 120
BstNI CCWGG 2 cut(s) 98, 227
BstSCI CCNGG 4 cut(s) 96, 225, 306, 334
BstUI CGCG 4 cut(s) 35, 121, 213, 351
BstX2I RGATCY 3 cut(s) 258, 312, 353
BstYI RGATCY 3 cut(s) 258, 312, 353
Bsu36I CCTNAGG 1 cut(s) 297
BsuRI GGCC 4 cut(s) 194, 333, 339, 481
BtgI CCRYGG 1 cut(s) 349
BtrI CACGTC 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 460
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 4 cut(s) 19, 173, 177, 581
CaiI CAGNNNCTG 1 cut(s) 136
CfoI GCGC 1 cut(s) 123
Cfr13I GGNCC 3 cut(s) 61, 332, 480
Cfr42I CCGCGG 1 cut(s) 352
CseI GACGC 6 cut(s) 24, 39, 98, 160, 202, 390
Csp6I GTAC 4 cut(s) 247, 406, 539, 553
CviAII CATG 1 cut(s) 544
CviQI GTAC 4 cut(s) 247, 406, 539, 553
DdeI CTNAG 1 cut(s) 297
DpnI GATC 6 cut(s) 79, 260, 314, 355, 536, 548
DpnII GATC 6 cut(s) 77, 258, 312, 353, 534, 546
DraI TTTAAA 1 cut(s) 397
DriI GACNNNNNGTC 1 cut(s) 67
Eam1104I CTCTTC 2 cut(s) 497, 515
Eam1105I GACNNNNNGTC 1 cut(s) 67
EarI CTCTTC 2 cut(s) 497, 515
EciI GGCGGA 2 cut(s) 71, 356
Eco130I CCWWGG 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 32
Eco47I GGWCC 1 cut(s) 61
Eco57I CTGAAG 1 cut(s) 436
Eco81I CCTNAGG 1 cut(s) 297
Eco88I CYCGRG 2 cut(s) 72, 389
EcoO109I RGGNCCY 1 cut(s) 61
EcoRII CCWGG 2 cut(s) 96, 225
EcoT14I CCWWGG 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 195
Esp3I CGTCTC 1 cut(s) 75
FaeI CATG 1 cut(s) 547
FaiI YATR 3 cut(s) 545, 561, 591
FatI CATG 1 cut(s) 543
FauI CCCGC 2 cut(s) 10, 356
FbaI TGATCA 1 cut(s) 546
FblI GTMKAC 3 cut(s) 201, 216, 303
Fnu4HI GCNGC 3 cut(s) 15, 33, 155
Fsp4HI GCNGC 3 cut(s) 15, 33, 155
FspBI CTAG 1 cut(s) 503
GlaI GCGC 1 cut(s) 122
GluI GCNGC 3 cut(s) 15, 33, 155
HaeIII GGCC 4 cut(s) 194, 333, 339, 481
HapII CCGG 3 cut(s) 9, 308, 336
HgaI GACGC 6 cut(s) 24, 39, 98, 160, 202, 390
HhaI GCGC 1 cut(s) 123
Hin1I GRCGYC 3 cut(s) 50, 152, 382
Hin1II CATG 1 cut(s) 547
Hin6I GCGC 1 cut(s) 121
HinP1I GCGC 1 cut(s) 121
HincII GTYRAC 1 cut(s) 304
HindII GTYRAC 1 cut(s) 304
HindIII AAGCTT 1 cut(s) 603
HinfI GANTC 1 cut(s) 231
HpaII CCGG 3 cut(s) 9, 308, 336
Hpy166II GTNNAC 4 cut(s) 202, 217, 304, 541
Hpy188I TCNGA 2 cut(s) 328, 358
Hpy188III TCNNGA 1 cut(s) 439
Hpy8I GTNNAC 4 cut(s) 202, 217, 304, 541
HpyAV CCTTC 1 cut(s) 382
HpyCH4IV ACGT 3 cut(s) 159, 204, 237
HpyCH4V TGCA 3 cut(s) 429, 458, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 120
HpyF3I CTNAG 1 cut(s) 297
HpySE526I ACGT 3 cut(s) 159, 204, 237
Hsp92I GRCGYC 3 cut(s) 50, 152, 382
Hsp92II CATG 1 cut(s) 547
HspAI GCGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 546
KspI CCGCGG 1 cut(s) 352
Kzo9I GATC 6 cut(s) 77, 258, 312, 353, 534, 546
LguI GCTCTTC 1 cut(s) 515
LmnI GCTCC 2 cut(s) 11, 105
MaeI CTAG 1 cut(s) 503
MaeII ACGT 3 cut(s) 159, 204, 237
MalI GATC 6 cut(s) 79, 260, 314, 355, 536, 548
MbiI CCGCTC 1 cut(s) 14
MboI GATC 6 cut(s) 77, 258, 312, 353, 534, 546
MboII GAAGA 4 cut(s) 442, 479, 484, 502
MflI RGATCY 3 cut(s) 258, 312, 353
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 2 cut(s) 398, 411
MmeI TCCRAC 2 cut(s) 351, 381
MseI TTAA 1 cut(s) 396
MspA1I CMGCKG 2 cut(s) 171, 351
MspI CCGG 3 cut(s) 9, 308, 336
MspR9I CCNGG 4 cut(s) 98, 227, 308, 336
MvaI CCWGG 2 cut(s) 98, 227
MvnI CGCG 4 cut(s) 35, 121, 213, 351
MwoI GCNNNNNNNGC 2 cut(s) 98, 120
NciI CCSGG 2 cut(s) 308, 336
NdeII GATC 6 cut(s) 77, 258, 312, 353, 534, 546
NlaIII CATG 1 cut(s) 547
NlaIV GGNNCC 3 cut(s) 63, 334, 355
NmeAIII GCCGAG 1 cut(s) 165
PaqCI CACCTGC 1 cut(s) 144
PciSI GCTCTTC 1 cut(s) 515
PcsI WCGNNNNNNNCGW 3 cut(s) 47, 210, 324
PfeI GAWTC 1 cut(s) 231
Pfl23II CGTACG 1 cut(s) 246
PflFI GACNNNGTC 1 cut(s) 92
PkrI GCNGC 3 cut(s) 16, 34, 156
PpuMI RGGWCCY 1 cut(s) 61
Psp5II RGGWCCY 1 cut(s) 61
Psp6I CCWGG 2 cut(s) 96, 225
PspGI CCWGG 2 cut(s) 96, 225
PspLI CGTACG 1 cut(s) 246
PspN4I GGNNCC 3 cut(s) 63, 334, 355
PspPI GGNCC 3 cut(s) 61, 332, 480
PspPPI RGGWCCY 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 136
PsuI RGATCY 3 cut(s) 258, 312, 353
PsyI GACNNNGTC 1 cut(s) 92
RsaI GTAC 4 cut(s) 248, 407, 540, 554
RsaNI GTAC 4 cut(s) 247, 406, 539, 553
SacII CCGCGG 1 cut(s) 352
SalI GTCGAC 1 cut(s) 302
SapI GCTCTTC 1 cut(s) 515
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 3 cut(s) 15, 33, 155
Sau3AI GATC 6 cut(s) 77, 258, 312, 353, 534, 546
Sau96I GGNCC 3 cut(s) 61, 332, 480
ScrFI CCNGG 4 cut(s) 98, 227, 308, 336
SduI GDGCHC 1 cut(s) 510
Sfr303I CCGCGG 1 cut(s) 352
SgrBI CCGCGG 1 cut(s) 352
SinI GGWCC 1 cut(s) 61
Sse9I AATT 2 cut(s) 398, 411
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 4 cut(s) 96, 225, 306, 334
StyI CCWWGG 1 cut(s) 195
TaiI ACGT 3 cut(s) 162, 207, 240
TaqI TCGA 4 cut(s) 87, 141, 303, 379
TasI AATT 2 cut(s) 398, 411
TatI WGTACW 1 cut(s) 552
TauI GCSGC 3 cut(s) 17, 35, 157
TfiI GAWTC 1 cut(s) 231
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 460
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 92
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 398
XmiI GTMKAC 3 cut(s) 201, 216, 303
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.