MD16G1004000.v1.1

Cell differentiation protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003117
Physical Location & Seq
Forward (+)
223175 .. 228373
5199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1004000.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCAAACCGGCCCCAGTCACTTTCATTAAATGTTCCATTCGGAGGTCCAAGCGTTTCAGCACCAAGCGTTACTGGAGCTCAAGCAAACAAAGATCGTAAAATGGCATCAGCAGAGCACTTGGTGCTTGACTTGAGTAATCCTGATCTTCGAGAAAACACGCTTCTTGAACTTTCAAAGAACAAGGAATTATTTCAAGATTTGGCTCCTTTCGTGTGGAATTCTTTTGGTACTATTGCTGCACTCATCCAGGAGATAGTTTCAATTTACCCTGTTCTATCACCACCAAATCTGACTCCGGCACAATCCAATCGAGTTTGCAATGCTCTTGCTCTTCTTCAGTGCGTAGCTTCCCACCCAGATACAAGAATGTTGTTCCTCAATGCTCATATACCTTTATATCTGTATCCTTTTCTCAATACATCAAGCAAGGCAAGACCATTTGAGTACTTAAGGCTTACGAGTTTAGGAGTCATTGGTGCCCTGGTGAAGGTTGATGATACTGAAGTTATTAGTTTCCTTCTGTCAACAGAAATAATTCCACTGTGCCTTCGCACTATGGAATTGGGCAGTGAACTCTCAAAAACAGTAGCCACATTTATAGTTCAGAAGATTTTGTTGGATGATATGGGCTTGGACTATATTTGTACTACAGCAGAGCGGTTTTTTGCTGTAGGTCGAGTTTTGGGGACCATGGTTGCAGCACTGGCTGATCAACCCTCATCACGCCTTTTGAAACACATCATTCGTTGCTATCTTCGATTGTCAGATAATCCAAGGGCTTGTGATGCTTTAAGAAGTTGCCTTCCAGATGTGTTACGAGAGGCTAGCTTTAACAATTTCCTTTGTGAAGATCCAACTACTAGGAGGTGGCTGCAACAACTGCTGCAAAATGTTGGAGTGAGCCGGATTCCTGCACTGCAGGGTGGGGGAGGATTTGATCACGTGATGGTGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
Rcd1

Protein Analysis

320

Amino Acids

35.13

Weight (kDa)

6.52

Isoelectric Point (pI)

52.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rcd1 PF04078 41 - 299 4.4e-123 Cell differentiation family, Rcd1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 479
AccBSI CCGCTC 1 cut(s) 661
AciI CCGC 1 cut(s) 661
AclWI GGATC 1 cut(s) 848
AcsI RAATTY 1 cut(s) 220
AcuI CTGAAG 2 cut(s) 323, 525
AcvI CACGTG 1 cut(s) 946
AdeI CACNNNGTG 1 cut(s) 124
AfaI GTAC 3 cut(s) 232, 449, 649
AfiI CCNNNNNNNGG 2 cut(s) 44, 490
AflII CTTAAG 1 cut(s) 451
AgsI TTSAA 5 cut(s) 170, 177, 197, 264, 736
AjnI CCWGG 2 cut(s) 249, 483
AjuI GAANNNNNNNTTGG 2 cut(s) 602, 634
AluBI AGCT 3 cut(s) 80, 350, 831
AluI AGCT 3 cut(s) 80, 350, 831
Alw21I GWGCWC 2 cut(s) 82, 120
AlwI GGATC 1 cut(s) 848
AoxI GGCC 1 cut(s) 11
ApeKI GCWGC 4 cut(s) 239, 701, 874, 886
ApoI RAATTY 1 cut(s) 220
Asp700I GAANNNNTTC 2 cut(s) 192, 537
AspS9I GGNCC 3 cut(s) 12, 47, 690
AsuHPI GGTGA 2 cut(s) 273, 499
AsuNHI GCTAGC 1 cut(s) 827
AvaII GGWCC 2 cut(s) 47, 690
BaeGI GKGCMC 1 cut(s) 484
BaeI ACNNNNGTAYC 2 cut(s) 492, 525
BanI GGYRCC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 82
BbrPI CACGTG 1 cut(s) 946
Bbv12I GWGCWC 2 cut(s) 82, 120
BbvI GCAGC 4 cut(s) 226, 713, 861, 873
BccI CCATC 1 cut(s) 943
BciT130I CCWGG 2 cut(s) 251, 485
BciVI GTATCC 1 cut(s) 417
BclI TGATCA 2 cut(s) 712, 940
BfaI CTAG 2 cut(s) 828, 864
BfmI CTRYAG 3 cut(s) 651, 672, 920
BfrI CTTAAG 1 cut(s) 451
BfuI GTATCC 1 cut(s) 417
BisI GCNGC 4 cut(s) 240, 702, 875, 887
BlsI GCNGC 4 cut(s) 241, 703, 876, 888
BmcAI AGTACT 1 cut(s) 449
Bme1390I CCNGG 2 cut(s) 251, 485
Bme18I GGWCC 2 cut(s) 47, 690
BmgT120I GGNCC 3 cut(s) 12, 47, 690
BmiI GGNNCC 4 cut(s) 14, 207, 481, 691
BmrFI CCNGG 2 cut(s) 251, 485
BmrI ACTGGG 1 cut(s) 10
BmsI GCATC 2 cut(s) 116, 778
BmtI GCTAGC 1 cut(s) 831
BmuI ACTGGG 1 cut(s) 10
BpmI CTGGAG 1 cut(s) 96
BpuEI CTTGAG 2 cut(s) 66, 154
BsaAI YACGTR 1 cut(s) 946
BsaJI CCNNGG 3 cut(s) 483, 693, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 490
Bse118I RCCGGY 1 cut(s) 9
Bse1I ACTGG 3 cut(s) 16, 79, 711
Bse3DI GCAATG 1 cut(s) 328
BseBI CCWGG 2 cut(s) 251, 485
BseDI CCNNGG 3 cut(s) 483, 693, 776
BseGI GGATG 2 cut(s) 246, 628
BseLI CCNNNNNNNGG 2 cut(s) 44, 490
BseMI GCAATG 1 cut(s) 328
BseNI ACTGG 3 cut(s) 16, 79, 711
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 4 cut(s) 226, 713, 861, 873
BsgI GTGCAG 2 cut(s) 225, 900
BshFI GGCC 1 cut(s) 13
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 2 cut(s) 82, 120
BsiSI CCGG 3 cut(s) 10, 299, 907
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 2 cut(s) 44, 490
BsmFI GGGAC 1 cut(s) 703
BsnI GGCC 1 cut(s) 13
Bsp1286I GDGCHC 3 cut(s) 82, 120, 484
Bsp143I GATC 5 cut(s) 94, 145, 712, 853, 940
Bsp19I CCATGG 1 cut(s) 693
BspACI CCGC 1 cut(s) 661
BspANI GGCC 1 cut(s) 13
BspLI GGNNCC 4 cut(s) 14, 207, 481, 691
BspMAI CTGCAG 1 cut(s) 924
BspOI GCTAGC 1 cut(s) 831
BspPI GGATC 1 cut(s) 848
BspQI GCTCTTC 1 cut(s) 339
BspT107I GGYRCC 1 cut(s) 479
BspTI CTTAAG 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 661
BsrDI GCAATG 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 9
BsrI ACTGG 3 cut(s) 16, 79, 711
BssAI RCCGGY 1 cut(s) 9
BssECI CCNNGG 3 cut(s) 483, 693, 776
BssMI GATC 5 cut(s) 94, 145, 712, 853, 940
BssT1I CCWWGG 2 cut(s) 693, 776
Bst2UI CCWGG 2 cut(s) 251, 485
Bst4CI ACNGT 2 cut(s) 546, 589
Bst6I CTCTTC 1 cut(s) 339
BstAFI CTTAAG 1 cut(s) 451
BstAPI GCANNNNNTGC 2 cut(s) 124, 883
BstBAI YACGTR 1 cut(s) 946
BstC8I GCNNGC 1 cut(s) 829
BstDSI CCRYGG 1 cut(s) 693
BstENI CCTNNNNNAGG 1 cut(s) 488
BstF5I GGATG 2 cut(s) 246, 628
BstKTI GATC 5 cut(s) 97, 148, 715, 856, 943
BstMBI GATC 5 cut(s) 94, 145, 712, 853, 940
BstMWI GCNNNNNNNGC 4 cut(s) 124, 707, 788, 883
BstNI CCWGG 2 cut(s) 251, 485
BstSCI CCNGG 2 cut(s) 249, 483
BstSFI CTRYAG 3 cut(s) 651, 672, 920
BstSLI GKGCMC 1 cut(s) 484
BstV1I GCAGC 4 cut(s) 226, 713, 861, 873
BstX2I RGATCY 1 cut(s) 853
BstYI RGATCY 1 cut(s) 853
BsuI GTATCC 1 cut(s) 417
BsuRI GGCC 1 cut(s) 13
BtgI CCRYGG 1 cut(s) 693
BtsCI GGATG 2 cut(s) 246, 628
BtsI GCAGTG 2 cut(s) 577, 917
BtsIMutI CAGTG 5 cut(s) 347, 542, 577, 704, 917
Cac8I GCNNGC 1 cut(s) 829
Cfr10I RCCGGY 1 cut(s) 9
Cfr13I GGNCC 3 cut(s) 12, 47, 690
Csp6I GTAC 3 cut(s) 231, 448, 648
CviAII CATG 1 cut(s) 694
CviQI GTAC 3 cut(s) 231, 448, 648
DpnI GATC 5 cut(s) 96, 147, 714, 855, 942
DpnII GATC 5 cut(s) 94, 145, 712, 853, 940
DraIII CACNNNGTG 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 80
Eco130I CCWWGG 2 cut(s) 693, 776
Eco24I GRGCYC 1 cut(s) 82
Eco47I GGWCC 2 cut(s) 47, 690
Eco53kI GAGCTC 1 cut(s) 80
Eco57I CTGAAG 2 cut(s) 323, 525
Eco72I CACGTG 1 cut(s) 946
EcoICRI GAGCTC 1 cut(s) 80
EcoNI CCTNNNNNAGG 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 249, 483
EcoT14I CCWWGG 2 cut(s) 693, 776
EcoT38I GRGCYC 1 cut(s) 82
ErhI CCWWGG 2 cut(s) 693, 776
FaeI CATG 1 cut(s) 697
FaiI YATR 8 cut(s) 390, 392, 400, 560, 602, 629, 642, 695
FaqI GGGAC 1 cut(s) 703
FatI CATG 1 cut(s) 693
FbaI TGATCA 2 cut(s) 712, 940
Fnu4HI GCNGC 4 cut(s) 240, 702, 875, 887
FokI GGATG 2 cut(s) 233, 635
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 4 cut(s) 240, 702, 875, 887
FspBI CTAG 2 cut(s) 828, 864
GluI GCNGC 4 cut(s) 240, 702, 875, 887
GsuI CTGGAG 1 cut(s) 96
HaeIII GGCC 1 cut(s) 13
HapII CCGG 3 cut(s) 10, 299, 907
Hin1II CATG 1 cut(s) 697
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HinfI GANTC 3 cut(s) 295, 471, 910
HpaII CCGG 3 cut(s) 10, 299, 907
HphI GGTGA 2 cut(s) 273, 499
Hpy166II GTNNAC 2 cut(s) 528, 575
Hpy188I TCNGA 4 cut(s) 44, 294, 609, 769
Hpy188III TCNNGA 5 cut(s) 143, 152, 167, 197, 809
Hpy8I GTNNAC 2 cut(s) 528, 575
HpyAV CCTTC 4 cut(s) 484, 530, 560, 815
HpyCH4III ACNGT 2 cut(s) 546, 589
HpyCH4IV ACGT 1 cut(s) 945
HpyCH4V TGCA 7 cut(s) 242, 321, 701, 877, 889, 917, 922
HpyF10VI GCNNNNNNNGC 4 cut(s) 124, 707, 788, 883
HpySE526I ACGT 1 cut(s) 945
Hsp92II CATG 1 cut(s) 697
Ksp22I TGATCA 2 cut(s) 712, 940
Kzo9I GATC 5 cut(s) 94, 145, 712, 853, 940
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 77, 211
Lsp1109I GCAGC 4 cut(s) 226, 713, 861, 873
LweI GCATC 2 cut(s) 116, 778
MaeI CTAG 2 cut(s) 828, 864
MaeII ACGT 1 cut(s) 945
MaeIII GTNAC 3 cut(s) 18, 70, 816
MalI GATC 5 cut(s) 96, 147, 714, 855, 942
MbiI CCGCTC 1 cut(s) 661
MboI GATC 5 cut(s) 94, 145, 712, 853, 940
MboII GAAGA 6 cut(s) 140, 326, 329, 622, 749, 863
MflI RGATCY 1 cut(s) 853
MhlI GDGCHC 3 cut(s) 82, 120, 484
MluCI AATT 7 cut(s) 188, 220, 264, 537, 563, 838, 955
MlyI GAGTC 2 cut(s) 289, 480
MmeI TCCRAC 3 cut(s) 600, 877, 881
MnlI CCTC 6 cut(s) 38, 389, 730, 817, 861, 926
MroXI GAANNNNTTC 2 cut(s) 192, 537
MseI TTAA 4 cut(s) 29, 452, 794, 834
MspCI CTTAAG 1 cut(s) 451
MspI CCGG 3 cut(s) 10, 299, 907
MspR9I CCNGG 2 cut(s) 251, 485
MvaI CCWGG 2 cut(s) 251, 485
MwoI GCNNNNNNNGC 4 cut(s) 124, 707, 788, 883
NcoI CCATGG 1 cut(s) 693
NdeII GATC 5 cut(s) 94, 145, 712, 853, 940
NheI GCTAGC 1 cut(s) 827
NlaIII CATG 1 cut(s) 697
NlaIV GGNNCC 4 cut(s) 14, 207, 481, 691
NmuCI GTSAC 1 cut(s) 18
PciSI GCTCTTC 1 cut(s) 339
PdmI GAANNNNTTC 2 cut(s) 192, 537
PfeI GAWTC 1 cut(s) 910
PfoI TCCNGGA 1 cut(s) 249
PkrI GCNGC 4 cut(s) 241, 703, 876, 888
PleI GAGTC 2 cut(s) 289, 479
PmaCI CACGTG 1 cut(s) 946
PmlI CACGTG 1 cut(s) 946
PpsI GAGTC 2 cut(s) 289, 479
Ppu21I YACGTR 1 cut(s) 946
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 249, 483
PspCI CACGTG 1 cut(s) 946
PspGI CCWGG 2 cut(s) 249, 483
PspN4I GGNNCC 4 cut(s) 14, 207, 481, 691
PspPI GGNCC 3 cut(s) 12, 47, 690
PstI CTGCAG 1 cut(s) 924
PsuI RGATCY 1 cut(s) 853
RsaI GTAC 3 cut(s) 232, 449, 649
RsaNI GTAC 3 cut(s) 231, 448, 648
SacI GAGCTC 1 cut(s) 82
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 4 cut(s) 29, 452, 794, 834
SatI GCNGC 4 cut(s) 240, 702, 875, 887
Sau3AI GATC 5 cut(s) 94, 145, 712, 853, 940
Sau96I GGNCC 3 cut(s) 12, 47, 690
ScaI AGTACT 1 cut(s) 449
SchI GAGTC 2 cut(s) 289, 480
ScrFI CCNGG 2 cut(s) 251, 485
SduI GDGCHC 3 cut(s) 82, 120, 484
SetI ASST 9 cut(s) 49, 82, 352, 397, 495, 679, 833, 872, 948
SfaNI GCATC 2 cut(s) 116, 778
SfcI CTRYAG 3 cut(s) 651, 672, 920
SinI GGWCC 2 cut(s) 47, 690
SmlI CTYRAG 3 cut(s) 81, 133, 451
SmoI CTYRAG 3 cut(s) 81, 133, 451
Sse9I AATT 7 cut(s) 188, 220, 264, 537, 563, 838, 955
SsiI CCGC 1 cut(s) 661
SspMI CTAG 2 cut(s) 828, 864
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 2 cut(s) 249, 483
StyI CCWWGG 2 cut(s) 693, 776
TaaI ACNGT 2 cut(s) 546, 589
TaiI ACGT 1 cut(s) 948
TaqI TCGA 4 cut(s) 151, 313, 679, 760
TasI AATT 7 cut(s) 188, 220, 264, 537, 563, 838, 955
TatI WGTACW 2 cut(s) 447, 647
TfiI GAWTC 1 cut(s) 910
Tru1I TTAA 4 cut(s) 29, 452, 794, 834
Tru9I TTAA 4 cut(s) 29, 452, 794, 834
TscAI CASTG 5 cut(s) 347, 549, 577, 711, 924
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 4 cut(s) 239, 701, 874, 886
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 1 cut(s) 15
TspRI CASTG 5 cut(s) 347, 549, 577, 711, 924
Vha464I CTTAAG 1 cut(s) 451
VpaK11BI GGWCC 2 cut(s) 47, 690
XagI CCTNNNNNAGG 1 cut(s) 488
XapI RAATTY 1 cut(s) 220
XmnI GAANNNNTTC 2 cut(s) 192, 537
XspI CTAG 2 cut(s) 828, 864
ZrmI AGTACT 1 cut(s) 449
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.