MD16G1156300.v1.1

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002758
Physical Location & Seq
Reverse (-)
12453796 .. 12454320
525 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1156300.v1.1.491

Sequence Viewer

Length: 426 bp
ATGGACGAATGTGGATGCCTGCTGATTTCAACTTCTTATTTTGAGAGGAAAACAATTCCAAGTTTTCGAAGAGATTTGGAGGCTGAATATGTAACGGATAAAGACATTGGTTTCTCCGTCGGACTTGGCCCAGATGGTGTTTGGCAGAACATTCGGTCATTGCTTCCATTGTCAGTTGTCCCAAAATCGTTACAGAATGGTTTCATGGCTTTGAAATTTGTTATGAAAAATGGCAAGAAGCATGCAATTTTCAGGGGTCTTGCAACAGTTGTAAATGAGACCGCTGTTAAGTTGAAAATTTCATTATGTCATGCATCTCGAATTCAAGGTCGTGATTCCTCTCTTGGAAGAAGTGAGAGCATAAATCCTGGGAGTTCTTTTATTTTGCCTTGGAGAAGTACGTCTAGTGATTCTGACCAGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

142

Amino Acids

15.56

Weight (kDa)

9.0

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 282
AcsI RAATTY 3 cut(s) 215, 297, 321
AfaI GTAC 1 cut(s) 400
AgsI TTSAA 4 cut(s) 30, 214, 295, 326
AjnI CCWGG 1 cut(s) 367
Alw26I GTCTC 1 cut(s) 272
AoxI GGCC 1 cut(s) 127
ApoI RAATTY 3 cut(s) 215, 297, 321
ArsI GACNNNNNNTTYG 2 cut(s) 313, 345
Asp700I GAANNNNTTC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 128
AsuII TTCGAA 1 cut(s) 67
BccI CCATC 1 cut(s) 128
BcgI CGANNNNNNTGC 2 cut(s) 134, 168
BciT130I CCWGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 272
BfaI CTAG 1 cut(s) 405
Bme1390I CCNGG 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 128
BmrFI CCNGG 1 cut(s) 369
BmsI GCATC 2 cut(s) 5, 323
Bpu14I TTCGAA 1 cut(s) 67
BsaI GGTCTC 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 368, 389
Bse1I ACTGG 1 cut(s) 418
Bse3DI GCAATG 1 cut(s) 158
BseBI CCWGG 1 cut(s) 369
BseDI CCNNGG 2 cut(s) 368, 389
BseGI GGATG 1 cut(s) 20
BseMI GCAATG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 418
BshFI GGCC 1 cut(s) 129
BslFI GGGAC 1 cut(s) 164
BsmAI GTCTC 1 cut(s) 272
BsmFI GGGAC 1 cut(s) 164
BsnI GGCC 1 cut(s) 129
Bso31I GGTCTC 1 cut(s) 272
Bsp119I TTCGAA 1 cut(s) 67
BspACI CCGC 1 cut(s) 282
BspANI GGCC 1 cut(s) 129
BspT104I TTCGAA 1 cut(s) 67
BspTNI GGTCTC 1 cut(s) 272
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 1 cut(s) 418
BssECI CCNNGG 2 cut(s) 368, 389
BssT1I CCWWGG 1 cut(s) 389
Bst2UI CCWGG 1 cut(s) 369
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 20, 243
BstF5I GGATG 1 cut(s) 20
BstMAI GTCTC 1 cut(s) 272
BstNI CCWGG 1 cut(s) 369
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 367
BsuRI GGCC 1 cut(s) 129
BtsCI GGATG 1 cut(s) 20
BtsIMutI CAGTG 1 cut(s) 425
Cac8I GCNNGC 2 cut(s) 20, 243
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 399
CviAII CATG 3 cut(s) 205, 242, 311
CviJI RGCY 3 cut(s) 83, 129, 209
CviKI_1 RGCY 3 cut(s) 83, 129, 209
CviQI GTAC 1 cut(s) 399
Eam1104I CTCTTC 1 cut(s) 64
EarI CTCTTC 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 389
Eco31I GGTCTC 1 cut(s) 272
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 367
EcoT14I CCWWGG 1 cut(s) 389
EcoT22I ATGCAT 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 389
FaeI CATG 3 cut(s) 208, 245, 314
FaiI YATR 7 cut(s) 90, 206, 224, 243, 307, 312, 362
FaqI GGGAC 1 cut(s) 164
FatI CATG 3 cut(s) 204, 241, 310
FokI GGATG 1 cut(s) 27
FspBI CTAG 1 cut(s) 405
HaeIII GGCC 1 cut(s) 129
Hin1II CATG 3 cut(s) 208, 245, 314
HinfI GANTC 2 cut(s) 335, 410
Hpy188I TCNGA 2 cut(s) 122, 415
Hpy188III TCNNGA 2 cut(s) 318, 332
Hpy99I CGWCG 1 cut(s) 122
HpyCH4III ACNGT 1 cut(s) 268
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 3 cut(s) 245, 263, 314
HpySE526I ACGT 1 cut(s) 401
Hsp92II CATG 3 cut(s) 208, 245, 314
LpnPI CCDG 5 cut(s) 32, 144, 238, 354, 381
LweI GCATC 2 cut(s) 5, 323
MaeI CTAG 1 cut(s) 405
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 2 cut(s) 91, 189
MboII GAAGA 2 cut(s) 81, 360
MluCI AATT 5 cut(s) 54, 215, 246, 297, 321
MmeI TCCRAC 1 cut(s) 100
MnlI CCTC 3 cut(s) 39, 73, 349
Mph1103I ATGCAT 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 200
MseI TTAA 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 369
MvaI CCWGG 1 cut(s) 369
NlaIII CATG 3 cut(s) 208, 245, 314
NsiI ATGCAT 1 cut(s) 316
NspI RCATGY 1 cut(s) 245
NspV TTCGAA 1 cut(s) 67
PaeI GCATGC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 200
PfeI GAWTC 2 cut(s) 335, 410
Psp6I CCWGG 1 cut(s) 367
PspGI CCWGG 1 cut(s) 367
PspPI GGNCC 1 cut(s) 128
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
SaqAI TTAA 1 cut(s) 288
Sau96I GGNCC 1 cut(s) 128
ScrFI CCNGG 1 cut(s) 369
SetI ASST 2 cut(s) 331, 404
SfaNI GCATC 2 cut(s) 5, 323
SfuI TTCGAA 1 cut(s) 67
SphI GCATGC 1 cut(s) 245
Sse9I AATT 5 cut(s) 54, 215, 246, 297, 321
SsiI CCGC 1 cut(s) 282
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 367
StyI CCWWGG 1 cut(s) 389
TaaI ACNGT 1 cut(s) 268
TaiI ACGT 1 cut(s) 404
TaqI TCGA 2 cut(s) 67, 319
TaqII GACCGA 1 cut(s) 144
TasI AATT 5 cut(s) 54, 215, 246, 297, 321
TfiI GAWTC 2 cut(s) 335, 410
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TscAI CASTG 1 cut(s) 425
TspDTI ATGAA 3 cut(s) 193, 239, 291
TspGWI ACGGA 2 cut(s) 106, 110
TspRI CASTG 1 cut(s) 425
XapI RAATTY 3 cut(s) 215, 297, 321
XceI RCATGY 1 cut(s) 245
XcmI CCANNNNNNNNNTGG 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 200
XspI CTAG 1 cut(s) 405
Zsp2I ATGCAT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.