RchiOBHm_Chr4g0407781

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002758
Physical Location & Seq
Forward (+)
30078127 .. 30080554
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37900

Sequence Viewer

Length: 843 bp
ATGATTCTTAGGGAAGGTGGCATGACACTTAAGACAAAGCTTCACTCTTTAAAAATCAAAGATGAGCTTCAAGGTCGTCTCTCTACAACCCCACAGTATTTAGCTTGTTCTGTGCTGAAGAGTGACAATTTAGTTTCATCCCCCGGCATAATTGATCCACATTGGAAAGAAATGTCTGTTTTACTTCATGAGGAGGATGATACTTCTACGGATGCTTTGCCAGATTTTATGTCTGTATCTGATGCAGGCTTTGGTTCACCAACTTCAGATACAGATTCATGTGTAACAACTGAGGACAGCAATGATGCTGCTGGATTTGCATCTGCTAAAGGCTTAGTCCTTGAAAAGAATCTTGTTATGGCAAGGTGCATTTCTGCTGAGGAATTTTATGAGGCGGAGGGTAGTGACTATTCCAATTTTGTCTTTCTCACCTTCTCGACTCTGAGTTCTAGTTCACCTGATTATGATGGTATTGACACACAGTTGAGCATCCGTATGTCAAAACTGGAATTCTTCTGCAACAGACCTACTTTGGTCGCTTTAATTGATTTTGGCCTGGACTTAAGCTCTGTGTACAGTGAGGAAAACACTGCAGACATTACTGAAGGTTCAGGTGACAAATCTTTGACGAACAAAGAAAAGACCGAAGAAATTGGGCATGTCAAAGGCTTGCTGGGTTATGGTAAAGGTCATGTAGTATTTTATTTGAACATGAACGTGGACAGTGTGACTGTGTTTCTCAACAAGGAGGATGCTTCTCTGCTTGCGACGTTTGTGCAAGAAAGTTTTGTGCTAGATCTGAAGGTCCACCCAAGTTCTCTTTCCATTGAAGGCACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

280

Amino Acids

30.48

Weight (kDa)

4.45

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 833
AciI CCGC 1 cut(s) 395
AclWI GGATC 1 cut(s) 149
AcsI RAATTY 2 cut(s) 383, 509
AcuI CTGAAG 4 cut(s) 137, 249, 624, 821
AfaI GTAC 1 cut(s) 575
AflII CTTAAG 2 cut(s) 29, 562
AgsI TTSAA 4 cut(s) 71, 344, 709, 830
AjnI CCWGG 1 cut(s) 555
AjuI GAANNNNNNNTTGG 2 cut(s) 805, 837
AluBI AGCT 4 cut(s) 40, 67, 104, 567
AluI AGCT 4 cut(s) 40, 67, 104, 567
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 149
AoxI GGCC 1 cut(s) 553
ApeKI GCWGC 1 cut(s) 308
ApoI RAATTY 2 cut(s) 383, 509
AspS9I GGNCC 1 cut(s) 805
AsuC2I CCSGG 1 cut(s) 144
AsuHPI GGTGA 4 cut(s) 249, 421, 447, 626
AvaII GGWCC 1 cut(s) 805
BanI GGYRCC 1 cut(s) 833
BbvCI CCTCAGC 1 cut(s) 378
BbvI GCAGC 1 cut(s) 295
BccI CCATC 1 cut(s) 461
BcgI CGANNNNNNTGC 2 cut(s) 757, 791
BciT130I CCWGG 1 cut(s) 557
BcnI CCSGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 3 cut(s) 450, 794, 841
BfmI CTRYAG 1 cut(s) 591
BfrI CTTAAG 2 cut(s) 29, 562
BglII AGATCT 1 cut(s) 796
BisI GCNGC 1 cut(s) 309
BlsI GCNGC 1 cut(s) 310
Bme1390I CCNGG 2 cut(s) 144, 557
Bme18I GGWCC 1 cut(s) 805
BmgT120I GGNCC 1 cut(s) 805
BmiI GGNNCC 1 cut(s) 835
BmrFI CCNGG 2 cut(s) 144, 557
BmsI GCATC 6 cut(s) 202, 232, 295, 329, 498, 742
Bpu10I CCTNAGC 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 144
BsaBI GATNNNNATC 1 cut(s) 319
BsaJI CCNNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 510
Bse3DI GCAATG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 319
BseBI CCWGG 1 cut(s) 557
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 5 cut(s) 137, 202, 217, 489, 757
BseJI GATNNNNATC 1 cut(s) 319
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 3 cut(s) 282, 369, 434
BseNI ACTGG 1 cut(s) 510
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 295
BseYI CCCAGC 1 cut(s) 673
BshFI GGCC 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 833
BsiSI CCGG 1 cut(s) 144
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsnI GGCC 1 cut(s) 555
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 2 cut(s) 154, 796
BspACI CCGC 1 cut(s) 395
BspANI GGCC 1 cut(s) 555
BspCNI CTCAG 3 cut(s) 283, 370, 435
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 835
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 1 cut(s) 149
BspT107I GGYRCC 1 cut(s) 833
BspTI CTTAAG 2 cut(s) 29, 562
BsrDI GCAATG 1 cut(s) 307
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 1 cut(s) 510
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 154, 796
Bst2UI CCWGG 1 cut(s) 557
Bst4CI ACNGT 5 cut(s) 96, 483, 578, 725, 733
Bst6I CTCTTC 1 cut(s) 113
BstAFI CTTAAG 2 cut(s) 29, 562
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 3 cut(s) 247, 671, 765
BstDEI CTNAG 5 cut(s) 8, 291, 334, 378, 443
BstF5I GGATG 5 cut(s) 137, 202, 217, 489, 757
BstKTI GATC 2 cut(s) 157, 799
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 2 cut(s) 154, 796
BstMWI GCNNNNNNNGC 1 cut(s) 317
BstNI CCWGG 1 cut(s) 557
BstNSI RCATGY 1 cut(s) 662
BstSCI CCNGG 2 cut(s) 142, 555
BstSFI CTRYAG 1 cut(s) 591
BstV1I GCAGC 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
BsuRI GGCC 1 cut(s) 555
BtsCI GGATG 5 cut(s) 137, 202, 217, 489, 757
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 3 cut(s) 583, 588, 730
Cac8I GCNNGC 3 cut(s) 247, 671, 765
CciI TCATGA 1 cut(s) 187
Cfr13I GGNCC 1 cut(s) 805
Csp6I GTAC 1 cut(s) 574
CviAII CATG 6 cut(s) 22, 188, 279, 659, 692, 712
CviJI RGCY 8 cut(s) 40, 67, 104, 249, 333, 555, 567, 669
CviKI_1 RGCY 8 cut(s) 40, 67, 104, 249, 333, 555, 567, 669
CviQI GTAC 1 cut(s) 574
DdeI CTNAG 5 cut(s) 8, 291, 334, 378, 443
DpnI GATC 2 cut(s) 156, 798
DpnII GATC 2 cut(s) 154, 796
DraI TTTAAA 1 cut(s) 51
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
EciI GGCGGA 1 cut(s) 410
Eco47I GGWCC 1 cut(s) 805
Eco57I CTGAAG 4 cut(s) 137, 249, 624, 821
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 1 cut(s) 555
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 6 cut(s) 25, 191, 282, 662, 695, 715
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FatI CATG 6 cut(s) 21, 187, 278, 658, 691, 711
Fnu4HI GCNGC 1 cut(s) 309
FokI GGATG 5 cut(s) 124, 209, 224, 476, 764
Fsp4HI GCNGC 1 cut(s) 309
FspBI CTAG 3 cut(s) 450, 794, 841
GluI GCNGC 1 cut(s) 309
GsaI CCCAGC 1 cut(s) 677
HaeIII GGCC 1 cut(s) 555
HapII CCGG 1 cut(s) 144
Hin1II CATG 6 cut(s) 25, 191, 282, 662, 695, 715
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 4 cut(s) 4, 275, 349, 439
HpaII CCGG 1 cut(s) 144
HphI GGTGA 4 cut(s) 249, 421, 447, 626
Hpy166II GTNNAC 5 cut(s) 257, 455, 574, 721, 808
Hpy188I TCNGA 4 cut(s) 241, 268, 444, 801
Hpy188III TCNNGA 2 cut(s) 188, 436
Hpy8I GTNNAC 5 cut(s) 257, 455, 574, 721, 808
Hpy99I CGWCG 1 cut(s) 772
HpyAV CCTTC 5 cut(s) 8, 442, 599, 796, 824
HpyCH4III ACNGT 5 cut(s) 96, 483, 578, 725, 733
HpyCH4IV ACGT 2 cut(s) 717, 770
HpyCH4V TGCA 6 cut(s) 245, 320, 369, 519, 593, 778
HpyF10VI GCNNNNNNNGC 1 cut(s) 317
HpyF3I CTNAG 5 cut(s) 8, 291, 334, 378, 443
HpySE526I ACGT 2 cut(s) 717, 770
Hsp92II CATG 6 cut(s) 25, 191, 282, 662, 695, 715
Kzo9I GATC 2 cut(s) 154, 796
Lsp1109I GCAGC 1 cut(s) 295
LweI GCATC 6 cut(s) 202, 232, 295, 329, 498, 742
MaeI CTAG 3 cut(s) 450, 794, 841
MaeII ACGT 2 cut(s) 717, 770
MaeIII GTNAC 5 cut(s) 122, 283, 404, 614, 727
MalI GATC 2 cut(s) 156, 798
MboI GATC 2 cut(s) 154, 796
MboII GAAGA 3 cut(s) 130, 505, 659
MflI RGATCY 1 cut(s) 796
MluCI AATT 7 cut(s) 127, 150, 383, 415, 509, 543, 651
MlyI GAGTC 1 cut(s) 433
MnlI CCTC 8 cut(s) 184, 187, 286, 373, 385, 391, 574, 742
MseI TTAA 4 cut(s) 30, 50, 542, 563
MslI CAYNNNNRTG 2 cut(s) 494, 716
MspCI CTTAAG 2 cut(s) 29, 562
MspI CCGG 1 cut(s) 144
MspR9I CCNGG 2 cut(s) 144, 557
MvaI CCWGG 1 cut(s) 557
MwoI GCNNNNNNNGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 144
NdeII GATC 2 cut(s) 154, 796
NlaIII CATG 6 cut(s) 25, 191, 282, 662, 695, 715
NlaIV GGNNCC 1 cut(s) 835
NmuCI GTSAC 4 cut(s) 122, 404, 614, 727
NspI RCATGY 1 cut(s) 662
PagI TCATGA 1 cut(s) 187
PfeI GAWTC 3 cut(s) 4, 275, 349
PkrI GCNGC 1 cut(s) 310
PleI GAGTC 1 cut(s) 433
PpsI GAGTC 1 cut(s) 433
Psp6I CCWGG 1 cut(s) 555
PspFI CCCAGC 1 cut(s) 673
PspGI CCWGG 1 cut(s) 555
PspN4I GGNNCC 1 cut(s) 835
PspPI GGNCC 1 cut(s) 805
PstI CTGCAG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 796
RsaI GTAC 1 cut(s) 575
RsaNI GTAC 1 cut(s) 574
RseI CAYNNNNRTG 2 cut(s) 494, 716
SaqAI TTAA 4 cut(s) 30, 50, 542, 563
SatI GCNGC 1 cut(s) 309
Sau3AI GATC 2 cut(s) 154, 796
Sau96I GGNCC 1 cut(s) 805
SchI GAGTC 1 cut(s) 433
ScrFI CCNGG 2 cut(s) 144, 557
SfaNI GCATC 6 cut(s) 202, 232, 295, 329, 498, 742
SfcI CTRYAG 1 cut(s) 591
SinI GGWCC 1 cut(s) 805
SmiMI CAYNNNNRTG 2 cut(s) 494, 716
SmlI CTYRAG 2 cut(s) 29, 562
SmoI CTYRAG 2 cut(s) 29, 562
Sse9I AATT 7 cut(s) 127, 150, 383, 415, 509, 543, 651
SsiI CCGC 1 cut(s) 395
SspMI CTAG 3 cut(s) 450, 794, 841
StyD4I CCNGG 2 cut(s) 142, 555
TaaI ACNGT 5 cut(s) 96, 483, 578, 725, 733
TaiI ACGT 2 cut(s) 720, 773
TaqI TCGA 1 cut(s) 437
TaqII GACCGA 1 cut(s) 659
TasI AATT 7 cut(s) 127, 150, 383, 415, 509, 543, 651
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 3 cut(s) 4, 275, 349
Tru1I TTAA 4 cut(s) 30, 50, 542, 563
Tru9I TTAA 4 cut(s) 30, 50, 542, 563
TscAI CASTG 3 cut(s) 583, 595, 730
TseFI GTSAC 4 cut(s) 122, 404, 614, 727
TseI GCWGC 1 cut(s) 308
Tsp45I GTSAC 4 cut(s) 122, 404, 614, 727
TspDTI ATGAA 4 cut(s) 126, 176, 267, 728
TspGWI ACGGA 2 cut(s) 224, 482
TspRI CASTG 3 cut(s) 583, 595, 730
Vha464I CTTAAG 2 cut(s) 29, 562
VpaK11BI GGWCC 1 cut(s) 805
XapI RAATTY 2 cut(s) 383, 509
XceI RCATGY 1 cut(s) 662
XspI CTAG 3 cut(s) 450, 794, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.