Rh4CG288800

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002758
Physical Location & Seq
Reverse (-)
54710689 .. 54752316
41628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG288800.1

Sequence Viewer

Length: 7815 bp
ATGTTCGAAGCACACGTCCTCCATTTGCTTAGAAGATATTTGGGGGAATATGTCGAAGGACTCTCTGTCGAAGCTTTAAGAATAAGTGTTTGGCAAGGTGATGTTGTTCTCAAAGATTTGAAATTAAAGGCAGAGGCCCTAAATTCACTAAAACTTCCAGTTACTGTAAAAGCTGGTTTTATTGGTACCATTACATTAAGGGTTCCATGGAAAAGTTTGGGCAAAGAACCTGTGATTGTTCTCATTGACCGTGTATTCATCCTTGCTCATCCTGTTACTGATGACAGGACTCTCAAGGAGGACAGAGACAAACTATTTGAAGCTAAACTTCAAGAGATTGAGGAAGCAGAGTCGGCAACTCTTGAAGCAATATCAAAGTCAAAGATAGGAAGTCAATCTAATGGAAATTCATGGCTAGGTTCCCTTATTGCTACTATTATTGGGAATCTAAAGATATCAATTAGCAATGTACATATAAGATATGAGGATTCGGTCAGTAATCCAGGACATCCTTTTTGTTCGGGTGCCACTTTAGCCAAACTTGCTGCTGTTACGATGGATGAGGAAGGGAATGAAACATTTGATACCAGTGGTGCTCTTGATAAGTTGCGAAAGTCATTGCAACTAGAGAGACTAGCAATGTATCATGATTCTGATAGTGTACCGTGGAAGATAGATAAGGGATGGGAGGATCTTACTCCAAAGGAATGGGTCGAGATTTTTGAAGATGGAATACGTGAACCTGTGGATGACCATGCAATGGTGTCTAAGTGGGCTATGAATCGCAAGTATTTGGTCTCACCTATAAATGGAGTTCTAAAGTATCACCGTGTAGGAAACCAAGAAAGAAATGATCCAGAGGTTCCATTTGAGAACGCTTCCCTTGCCCTAAGTGACGTTTCCTTGACAATGACAGAGGCTCAATATTGTGATTGGATAAAACTATTGGAAGTTGTTTCAAGATACAAGAGATATGTTGAATTTTTCCATCTAAGACCAGCGCTACCAGTCTCAGGGGATCCATATTTGTGGTGGCGTTATGCTGCTCAAGCAGGACTTCAGCAGAAGAAAATGTGTTACCGGTTCTCTTGGGACCGCATTGGGCTTCTTTGCCAGCTTCGTCGCCGTTACATTCAGTTATATGTTGGTTTCTTACAACACCTGACAAATGTTGATAACGCTGAAATTAGAGAAATTGAGAAAGATTTGGATCCTAAAGTAATTCTTCTGTGGAGGTTACTTGCACATGCAAAGGTCGAATCTGTAAAATCGAAGGAAGCGGCTGAACAGAGGATGCTCCAGAAGAAAAGCTGGTTTTCATTTAGATGGCGTACACCTGATGAATCTGCTGATGATACTTCTGAGGGATCACAGTCACCAGAAGAAAGACTGACTAAAGAGGAGTGGCAAGCAATTAATAAGTTACTGAGTTACCAGCCAGATGAAACATTGGCATCTCACTCGGGAAAGGACATGCCGAATATGATCCGGTTTATGGTAACTGTGTCTGTTGATCGGGCTGCTGCTAGGATCATTGATATAAATCACACTGAGATTTTGTGTTGTAAATTTGAGCAACTTCAAGTGTCAACGAAGTTTAAACATAAAAGTACATACTGTGATGTGTCATTGAGATTCTATGGTCTGTATGCACCGGAAGGCTCACTTGCTCAGAGCGTATCTAGTGAGCAGAAGGCTAATGCACTGGCTGCTAGCTTTGTGTACTCTCCAGTTGGAGAGAAGGCTTTCAGCTACCATTTCACCTTGTCATGTCACTTTAGTCCTACTGTTACATTGGAAACGGCAACTGCTTTGCAGATGCAAATTGAGAAAGTGACTCGTAGAGCACAAGAGCAATTTCAAATGGTATTAGAAGAACAAAGCAGATTTGCCCTTGATATTGATTTGGATGCTCCAAAAGTAAGAGTTCCTATCAGAACCAAGGGCTCTTCCAAATGTGATAGCCATTTTCTATTGGATCTTGGTCATTTCACCCTGCAAACGAAGGATACTCAGCATGATGAACAGAAGAACATGTATTCTCGTTTTTTTATAACGGGAAGAGATATTGCTGCATTTTTTACGGAATGTGGTTCTGATTGCCAAATTCGCACTTTGGAATCACCTGATTCTGATAACCACCCACCGGTTACCCCGGAAAATGTTGACAATTTCTATCCTCTCATAGATAGGTGTGGAATGGCTCTTCTTGTTGATCAGATCAAAGTACCTCACCCAATTTACCCGTCAATGCGTATTTTAATTCAAGTGTCAAACCTTGGGGTGCACTTTTCACCATCGAGATTTCAGAGACTCATGAAATTATTAAACATATTTTATGGTACTCTGGAAACTGTTGGTCAGCCGGCTGTTGATAATTTTCAAGCAGAACGCGCCCCATGGAGTCCTGCAGATCTTTACACAGATGCTAGAATTTTAGTATGGAAGGGGATTGGCAATTCTGTAGCTACGTGGCAACCTTGTATTCTTGTGTTGTCGGGGACAAATATTTTTGTTTTGGAGTCTGAGAAGTCGCAGACTTATCAGCGATACTCCAGCATGGCTGGTAGACAAGTATGTGAGGTTCCTCCTACAAGTATTGGTGGTTCACCATTTGGCCTTGCTGTGATTCACAGGGGGATGGACACTCAAAAGGCTCTGGAGTCTTCTAGTAGCTTGATTATTGAGTTCCGTGGGGAGGAGGAGAAGGTCGTTTGGTTGAAAAGACTCATACAAGCAACATATCAAGCTTCTGCTCCCCCTTCAGTTGATGTCTTGGGAGAAACAATTGATCGTGTTGCTGAGTTCGGTGAGCCTCAGACCGGCAATTCTAAAACAGCTGACCTTGTCATTAATGGGGCATTGGTGGAGACAAAGTTATCTATTTATGGAAAGACGGGTGATAAAGATGCTGAAGAACTTGATGAAACTCTGATGCTTGAGGTTCTTGCCAGTGGGGGGCAGGTTCATATGATTCTTTGGGAAGGTGACATGACACTTAAGACAAAGCTTCACTCTTTAAAAATCAAAGATGAGCTTCAAGGTCGTCTCTCTACAACCCCACAGTATTTAGCTTGTTCTGTGCTGAAGAGTGACAATTTAGTTTCATCCCCCGGCATAATTGATCCACGTTGGAAAGAAATGTCTGTTTTACTTCATGAGGAGGATGATACTTTTACGGATGCTTTGCCAGATTTTATGTCTGTATCAGATGCAGGCTTTGGTTCACCAACTTCAGATATGGTTTCATGTGTAACAACTGAGGACATCAATGATGCTGCTGGATTTACATCTGCTGAAGGCTTAGTCCTTGAAAAGAATCTTGTTAAGGCAAGTTCACTTCATTATGATGGTATTGACACACAGATGAGCATCCGTATGTCAAAACTGGAATTCTTCTGCAACAGACCTACTTTGGTCGCTTTAATTGATTTTGGCCTGGACTTAAGCTCTGTGTACAGTGAGGAAAGTACTGCAGACATTACTGAAGGTTCAGGTGACAAATCTTTGACGAACAAAGAAAAGACTGAAGAAATTGGGCGTGTCAAAGGCTTGCTGGGTTATGGTAAAGGTCGTGTAGTATTTTATTTGAACATGAACGTGGACAGTGTGACTGTGTTTCTCAACAAGGAGGATGCTTCTCTGCTTGCGACGTTTGTGCAAGAAAGTTTTGTGCTAGATCTGAAGGTCCACCCAAGTTCTCTTTCCATTGAAGGCACCCTAGGAAATTTTAGACTCCGTGATATGTCTCTTGGAGAAGATAACTGTTGGGCTTGGCTATGTGACATACGCAATCCAGACGTGGAATCCCTTATCAAGTTCAACTTTAAGTCTTACAGTGCTGAAGACGATGATTATGAAGGATATGATTATAGTTTGTGTGGTCGATTTGCTGCTGTTCGTATTGTCTTCCTTTACAGGTTTGTTCAGGAGATAACCGCATACTTTATGGAACTTGCTACCCCACATACTGAAGATGCAATCAAACTTGTTGATAAGGTTGGGGGTTTTGAGTGGCTAATTCAGAAGTATGAGATGGATGGAGCTGCCGCACTTAAGCTGGATCTGTCACTCGATACTCCAATAATCATTGTTCCCAGGAATTCAACTAGCAATGATTTCATTCAACTTGATCTGGGCCAGCTACAAGTCACAAATGAATTTAGTTGGCATGGTTCTCCAGAGAAAGATCCTTCTGCAGTCCACATTGATGTACTTCATGCTGAGATTCTGGGGATCAATATGTCTGTAGGAATCGACGGTCGCATGGGTAAATCAATGATACGAGAAGGCAAAGGATTTGATGTTTATGTCCGGAGAAGTTTGAGGGATATCTTCAAAAAAGTCCCAACATTTTCTCTTGAAGTGAAGGTGGGCTTATTGCGTTGCGTCATGTCTGACAAAGAATACAAAGTCATTCTGGATTGTGCATACACGAACTTATGTGAAGAACCAAAGCTTCCCCCTAGTTTCCGTGGTGGAATATCTGATTCAAAGGATACAATTAGGCTGCTGGTTGATAAGGTCAACATGAATAGTCAAATCCTACTATTGCGGACTGTTTCGATAGTGGCGGTTGTGGTTGATCATGCACTGTTGGAGTTGTATAATGGTCTTCATGTGGAGTCTCCTTTTGCTCAAATTGCTCTTGAAGGTCTTTGGGTGTCGTATCGGATGACTTCATTGTCTGAAACAGATCTGTACATAACAATTTCAAAATTTTCTGTTGTGGACATTCGCCCTGATACAAAACCTGAAATGCGCCTTATGCTTGGATCATCCACGGATGCTTTTAAACAAGTTTCCTCTGGAAGTTTGCCTTTCTTCTTAAATAGGGGGAGCTTTAGGAGAACTGACTCTGATGCTGGATTCCATGGGGACTTACCAATATCAACAATGTTCTTAATGGACTATAGATGGCGCACATCAACCCAATTGTTTGTGGTTAGGATTCAGCAACCTCGAGTTCTAGTTGTAGCCGATTTCCTTTTGGCTGTGGGCGAGTTTTTTGTTCCTGCATTGCGGACAATAACTGGTAGGGAGGAAGTGATGGATCCTCATAATGATCCTATTGGTAAAAACAGTAGCATAGTGTTCACAGAGCCTACATACAAACAGACAGAAGATGTGGTGCATTTGTCTCCAAGTAGACAGTTAGTAGCAGATTTTTTAAGCATCGATGAATATACATATGATGGTTGTGGAAAGACCATCCATTTGAGTGAGGAAATAGATGCTAAAGAACTCCATTCAACCAGACCTCGACCAATAATTATAATTGGACGTGGAAAAAGGTTGCGGTTTATGAATGTCAAAATTGAGAATGGGTCTCTTTTGAGGAAGTATACATACTTAAATAATGATAGCAGCTACTCAGTAGCCTTTGAGGATGGTGTTGACATTACGTTGCTGGAAAGTTCCTCATCTGATGATGATAAGAAGAGTCTAGAATACTTCCATGAATCGCCAGACTCCTCAAATATTTCCTCAAATTCTGAAAGTGATCCTAATAAGATCCCAAGTTTCAGTTTTGAAACACAGGTTGTTTCTCCTGAGTTTACTTTTTATGATGGTTCAAAGTCGTCTCTGGATGATTCATATGGTGAAAAGCTTCTCCGTGCAAAGCTGGATTTTAGCTTCATGCCAGTTCCTCCCTCACAGGCAGTAATGGCAGTGAGTAATACATATGGGCGTGTGTGTAAACCAACTGGTTTCAATCTCATAGGATTGTTCTCTGCAATTCAAGGATTTACAGGGGGTGACTCTGATATTAATACAGATTGTTCTCTTTGGATGCCTATAGCACCACCAGGATACACAGCATTGGGTTCTGTAGCAAATATTGGGAAGGAACCCCCACCCAACCACATTGTTTATTGCATTCGTTCCGATCTTGTAACATCAACAACCTATATAGAATGTTTGTTCTGCTCTGCATCAAATCCTCAATTTGCATCTGGATTTAGCATCTGGCGTGTAGAGAATGTGCTTGGATCATTTTATGCCCATTCTTCTACTGAATGTCCTTCCCAAGACCGTTGTTGTAACCTCAATCATCTTCTTCTTTGGAACTCAAGTCGGCATCATTCTTCTGCTAAAGAACCTGCCTCAGATTTAGCTGTTGTTGAAAACCGTGAAAGTCAGGTAAGCAGAAACCAGAGTGCACATTCTTCTGGATGGGATATTGTCAGATCAAGTTCAAGGACAACTAAATGTTATATGTCTACCCCTAACTTTGAGAGAATCTGGTGGGACAAGGGTAGTGACATTCGCCGACCAGTCTCAATATGGAGACCCATTCCTCGTCAAGGCTATGCCATCTTGGGTGATTGCATTACTGAAGGCTTAGAACCACCAGCTGTTGGCATAATATTTAGAGCTGATGATTGTGAAATATCTGCGGAACCCGTGCAATTTACTAAAGTTGCCCATGTGGTTGGGAAAGGTTTTGATGAAGCTTTCTTCTGGTACCCAATTGCTCCACCAGGTTATGCTTCTCTAGGATGCATAGTCTCCAGAATAGATGAACCCCCAGGTGTAAACTCATTTTGCTGCCCTAGGATGGATCTGGTTAACCAAGCTAATATTCTTGAGGTGCCCATTTCAAGATCTTCAGCTTCAAAGGGTTCTCAGTGCTGGAGCATCTGGAGAGTTGAAAATCAGGCATGCACTTTTCTTGCACGTGGTGATCTGAAAAAACCGTCTAGCAGATTGGCTTATGTTATTGGTGACTCCATGAAACCCAAGACTAAGGAAAACATAACTGCTGAGGTGAAGCTAAGATGCTTGTCTGTAACTGTTCTAGACAGCTTGTGTGGAATGATGACCCCACTATTTGACACAACAATCACCAATATCAAACTTGCAACACATGGTCGAGTAGAAGCCATGAATGCAGTACTAATTTCCTCTATTGCTGCATCAACCTTCAATACTCAGTTGGAAGCGTGGGAGCCGCTTGTGGAACCTTTTGATGGAATATTCAAGTTTGAAACATATGATTCTAATGTACACTCGCCATCAGAATTTGGGAAGAAAATGCGTATTGCTGCCACAAGTATCGTTAATATAAATGTCAGCGCCGCAAATCTTGAAACTTTTGTAGGGACTATTCTGTCATGGAGAAGACAATTAGACCTTGAACAGAAAGCAAAGAAGATAAATGAGGAAGCTTGTGGTCTAAGCGGTAAAGGAGAGGACCAGACTTTGTCTGCACTGGATGATGACGACTTTCAGACTGTGATAGTAGAGAATAAACTTGGTTGTGATGTCTATCTGAAAAAAGTTGAACAAAATTCGGATATGGTGAACCAGTTGCATCATGGTGACTCTGTCTCTGTATGGGTTCCACCTCCACAGTTTTCAGACAGGTTAAATGTTGCAGATGAATCCAAGGAAGCTCGTTATTATGTTGCTATACAAATTCATGAAGCTAAGGGTTTGCCTATTACAGATGATGGTAATAGCCATAACTTCTTATGTGCTTTGAGACTTGCTGTTGATAACCAGGCAACAGATCAACAAAAACTTTTTCCTCAAAGTGCAAGGACAAAATGCGTTAAGCCTTCAGTTTCGAAAAATAGTAACCTGAATGAAGGCTCTGCAGAATGGAATGAACTTTTCATATTTGAAGTTCCTCGGAAGATCGCCCTACATCTCTTTAAGAGCCAAAGTGTAGGCGGATACATTGGGAAAAGTATCCATAGTTTCATTGGCGGATACGTAGTATTGAGTGTGCAAGTGTTGCAGGGCCCAGCTAAACGGGAAGTGGAGGTGACAAACCTCGCAGCAAAAGCTGGAAAGGGTAAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

2604

Amino Acids

291.31

Weight (kDa)

5.4

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 1 - 537 1.3e-90 VPS13-like family N-terminal region
ATG2_CAD PF13329 8 - 166 3.1e-08 Autophagy-related protein 2
VPS13_M PF25033 1350 - 1488 4.8e-07 VPS13-like family middle region
Vps62 PF06101 1922 - 1993 9.3e-06 Vacuolar protein sorting-associated protein 62
Vps62 PF06101 2079 - 2219 1.1e-08 Vacuolar protein sorting-associated protein 62
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2054, 5276
AasI GACNNNNNNGTC 2 cut(s) 4684, 7114
Acc36I ACCTGC 2 cut(s) 2953, 6112
Acc65I GGTACC 2 cut(s) 185, 6468
AccB1I GGYRCC 5 cut(s) 185, 524, 3716, 6468, 6595
AccB7I CCANNNNNTGG 2 cut(s) 760, 6362
AccI GTMKAC 4 cut(s) 2569, 5149, 5345, 6224
AccII CGCG 1 cut(s) 2394
AccIII TCCGGA 1 cut(s) 4314
AcvI CACGTG 1 cut(s) 6683
AdeI CACNNNGTG 1 cut(s) 6686
AfeI AGCGCT 1 cut(s) 1002
AflII CTTAAG 3 cut(s) 2999, 3445, 4055
AflIII ACRYGT 2 cut(s) 13, 2034
AgeI ACCGGT 2 cut(s) 1080, 2146
AhdI GACNNNNNGTC 1 cut(s) 65
AjiI CACGTC 3 cut(s) 16, 3802, 5285
AjnI CCWGG 7 cut(s) 502, 3438, 4097, 5809, 6484, 6532, 7506
AjuI GAANNNNNNNTTGG 6 cut(s) 73, 105, 3688, 3720, 6570, 6602
Alw21I GWGCWC 4 cut(s) 598, 1849, 2289, 6166
Alw44I GTGCAC 2 cut(s) 2285, 6162
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 2 cut(s) 1462, 4962
Aor13HI TCCGGA 1 cut(s) 4314
Aor51HI AGCGCT 1 cut(s) 1002
AoxI GGCC 5 cut(s) 135, 2617, 3436, 4138, 7750
ApaI GGGCCC 1 cut(s) 7754
ApaLI GTGCAC 2 cut(s) 2285, 6162
ArsI GACNNNNNNTTYG 7 cut(s) 32, 299, 331, 498, 530, 5200, 5232
AseI ATTAAT 3 cut(s) 1416, 2853, 5772
AsiGI ACCGGT 2 cut(s) 1080, 2146
Asp700I GAANNNNTTC 2 cut(s) 1745, 5610
Asp718I GGTACC 2 cut(s) 185, 6468
AspA2I CCTAGG 2 cut(s) 3721, 6557
AspLEI GCGC 5 cut(s) 1003, 2396, 4764, 4923, 7082
AspS9I GGNCC 7 cut(s) 136, 1093, 3688, 4138, 7198, 7750, 7751
AsuC2I CCSGG 2 cut(s) 2156, 3114
AsuII TTCGAA 2 cut(s) 6, 7574
AsuNHI GCTAGC 1 cut(s) 1712
AvaI CYCGRG 2 cut(s) 1462, 4962
AvaII GGWCC 3 cut(s) 1093, 3688, 7198
AvrII CCTAGG 2 cut(s) 3721, 6557
BaeGI GKGCMC 4 cut(s) 2289, 6166, 6600, 7754
BamHI GGATCC 3 cut(s) 1018, 1210, 5053
BanI GGYRCC 5 cut(s) 185, 524, 3716, 6468, 6595
BanII GRGCYC 2 cut(s) 1949, 7754
BarI GAAGNNNNNNTAC 4 cut(s) 717, 749, 5333, 5365
BbrPI CACGTG 1 cut(s) 6683
BbsI GAAGAC 5 cut(s) 2658, 3852, 3901, 4607, 7132
Bbv12I GWGCWC 4 cut(s) 598, 1849, 2289, 6166
BbvCI CCTCAGC 1 cut(s) 6768
BceAI ACGGC 2 cut(s) 1110, 1818
BcgI CGANNNNNNTGC 4 cut(s) 1443, 1477, 3640, 3674
BciT130I CCWGG 7 cut(s) 504, 3440, 4099, 5811, 6486, 6534, 7508
BciVI GTATCC 6 cut(s) 2002, 4492, 5807, 7676, 7709, 7712
BclI TGATCA 2 cut(s) 2215, 4585
BcnI CCSGG 2 cut(s) 2156, 3114
BfmI CTRYAG 9 cut(s) 2409, 2463, 3474, 4197, 4248, 4912, 5799, 5832, 7602
BfoI RGCGCY 2 cut(s) 1004, 7083
BfrI CTTAAG 3 cut(s) 2999, 3445, 4055
BfuAI ACCTGC 2 cut(s) 2953, 6112
BfuI GTATCC 6 cut(s) 2002, 4492, 5807, 7676, 7709, 7712
BglII AGATCT 4 cut(s) 2413, 3679, 4696, 6608
BlnI CCTAGG 2 cut(s) 3721, 6557
BmcAI AGTACT 3 cut(s) 3472, 6900, 7811
Bme1390I CCNGG 9 cut(s) 504, 2156, 3114, 3440, 4099, 5811, 6486, 6534, 7508
Bme18I GGWCC 3 cut(s) 1093, 3688, 7198
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 2 cut(s) 1462, 4962
BmgBI CACGTC 3 cut(s) 16, 3802, 5285
BmgT120I GGNCC 7 cut(s) 136, 1093, 3688, 4138, 7198, 7750, 7751
BmrFI CCNGG 9 cut(s) 504, 2156, 3114, 3440, 4099, 5811, 6486, 6534, 7508
BmtI GCTAGC 1 cut(s) 1716
BpiI GAAGAC 5 cut(s) 2658, 3852, 3901, 4607, 7132
BpmI CTGGAG 8 cut(s) 1283, 1713, 2539, 2681, 4164, 6499, 6658, 6667
Bpu10I CCTNAGC 2 cut(s) 6768, 7434
Bpu14I TTCGAA 2 cut(s) 6, 7574
BpuEI CTTGAG 5 cut(s) 278, 1032, 2960, 6058, 6611
BpuMI CCSGG 2 cut(s) 2156, 3114
Bsa29I ATCGAT 1 cut(s) 5178
BsaAI YACGTR 4 cut(s) 737, 2472, 6683, 7723
BsaBI GATNNNNATC 5 cut(s) 1209, 1542, 3289, 3371, 7272
BsaI GGTCTC 3 cut(s) 802, 5334, 6286
BsaWI WCCGGW 5 cut(s) 1080, 1488, 1654, 2146, 4314
BsaXI ACNNNNNCTCC 3 cut(s) 33, 2861, 2891
Bse118I RCCGGY 4 cut(s) 1080, 2146, 2365, 2822
Bse3DI GCAATG 6 cut(s) 472, 617, 645, 765, 4120, 5018
Bse8I GATNNNNATC 5 cut(s) 1209, 1542, 3289, 3371, 7272
BseAI TCCGGA 1 cut(s) 4314
BseBI CCWGG 7 cut(s) 504, 3440, 4099, 5811, 6486, 6534, 7508
BseCI ATCGAT 1 cut(s) 5178
BseJI GATNNNNATC 5 cut(s) 1209, 1542, 3289, 3371, 7272
BseMI GCAATG 6 cut(s) 472, 617, 645, 765, 4120, 5018
BseRI GAGGAG 5 cut(s) 1415, 2714, 2717, 3176, 5464
BseSI GKGCMC 4 cut(s) 2289, 6166, 6600, 7754
BseYI CCCAGC 2 cut(s) 3556, 7753
BsgI GTGCAG 1 cut(s) 7197
Bsh1236I CGCG 1 cut(s) 2394
Bsh1285I CGRYCG 1 cut(s) 4264
BshFI GGCC 5 cut(s) 137, 2619, 3438, 4140, 7752
BshNI GGYRCC 5 cut(s) 185, 524, 3716, 6468, 6595
BshTI ACCGGT 2 cut(s) 1080, 2146
BshVI ATCGAT 1 cut(s) 5178
BsiEI CGRYCG 1 cut(s) 4264
BsiHKAI GWGCWC 4 cut(s) 598, 1849, 2289, 6166
BsiHKCI CYCGRG 2 cut(s) 1462, 4962
BsiSI CCGG 9 cut(s) 1081, 1489, 1655, 2147, 2156, 2366, 2823, 3114, 4315
BslFI GGGAC 6 cut(s) 1106, 2515, 4331, 4892, 6266, 7120
BsmBI CGTCTC 2 cut(s) 3053, 5589
BsmFI GGGAC 6 cut(s) 1106, 2515, 4331, 4892, 6266, 7120
BsmI GAATGC 2 cut(s) 5880, 6898
BsnI GGCC 5 cut(s) 137, 2619, 3438, 4140, 7752
Bso31I GGTCTC 3 cut(s) 802, 5334, 6286
BsoBI CYCGRG 2 cut(s) 1462, 4962
Bsp119I TTCGAA 2 cut(s) 6, 7574
Bsp120I GGGCCC 1 cut(s) 7750
Bsp1286I GDGCHC 7 cut(s) 598, 1849, 1949, 2289, 6166, 6600, 7754
Bsp13I TCCGGA 1 cut(s) 4314
Bsp1407I TGTACA 4 cut(s) 469, 3456, 4701, 7009
Bsp19I CCATGG 3 cut(s) 206, 2399, 4873
BspANI GGCC 5 cut(s) 137, 2619, 3438, 4140, 7752
BspDI ATCGAT 1 cut(s) 5178
BspEI TCCGGA 1 cut(s) 4314
BspFNI CGCG 1 cut(s) 2394
BspHI TCATGA 4 cut(s) 646, 2316, 3157, 7426
BspMAI CTGCAG 4 cut(s) 2413, 3478, 4201, 7606
BspMI ACCTGC 2 cut(s) 2953, 6112
BspOI GCTAGC 1 cut(s) 1716
BspQI GCTCTTC 2 cut(s) 1954, 2211
BspT104I TTCGAA 2 cut(s) 6, 7574
BspT107I GGYRCC 5 cut(s) 185, 524, 3716, 6468, 6595
BspTI CTTAAG 3 cut(s) 2999, 3445, 4055
BspTNI GGTCTC 3 cut(s) 802, 5334, 6286
BsrDI GCAATG 6 cut(s) 472, 617, 645, 765, 4120, 5018
BsrFI RCCGGY 4 cut(s) 1080, 2146, 2365, 2822
BsrGI TGTACA 4 cut(s) 469, 3456, 4701, 7009
BssAI RCCGGY 4 cut(s) 1080, 2146, 2365, 2822
BssNAI GTATAC 1 cut(s) 5346
BssT1I CCWWGG 8 cut(s) 206, 1941, 2278, 2399, 3721, 4873, 6557, 7392
Bst1107I GTATAC 1 cut(s) 5346
Bst2UI CCWGG 7 cut(s) 504, 3440, 4099, 5811, 6486, 6534, 7508
Bst6I CTCTTC 5 cut(s) 1954, 2056, 2211, 3083, 5435
BstAFI CTTAAG 3 cut(s) 2999, 3445, 4055
BstAPI GCANNNNNTGC 2 cut(s) 1709, 7744
BstAUI TGTACA 4 cut(s) 469, 3456, 4701, 7009
BstBAI YACGTR 4 cut(s) 737, 2472, 6683, 7723
BstBI TTCGAA 2 cut(s) 6, 7574
BstC8I GCNNGC 9 cut(s) 1115, 1410, 1714, 2367, 3217, 3554, 3648, 4142, 6667
BstDSI CCRYGG 7 cut(s) 206, 665, 2399, 2692, 4474, 4782, 4873
BstEII GGTNACC 1 cut(s) 2149
BstENI CCTNNNNNAGG 1 cut(s) 6306
BstFNI CGCG 1 cut(s) 2394
BstH2I RGCGCY 2 cut(s) 1004, 7083
BstHHI GCGC 5 cut(s) 1003, 2396, 4764, 4923, 7082
BstMCI CGRYCG 1 cut(s) 4264
BstNI CCWGG 7 cut(s) 504, 3440, 4099, 5811, 6486, 6534, 7508
BstNSI RCATGY 4 cut(s) 1250, 1477, 2038, 6669
BstPI GGTNACC 1 cut(s) 2149
BstSCI CCNGG 9 cut(s) 502, 2154, 3112, 3438, 4097, 5809, 6484, 6532, 7506
BstSFI CTRYAG 9 cut(s) 2409, 2463, 3474, 4197, 4248, 4912, 5799, 5832, 7602
BstSLI GKGCMC 4 cut(s) 2289, 6166, 6600, 7754
BstSNI TACGTA 1 cut(s) 7723
BstUI CGCG 1 cut(s) 2394
BstV2I GAAGAC 5 cut(s) 2658, 3852, 3901, 4607, 7132
BstXI CCANNNNNNTGG 3 cut(s) 708, 1029, 6437
BstZ17I GTATAC 1 cut(s) 5346
Bsu15I ATCGAT 1 cut(s) 5178
BsuI GTATCC 6 cut(s) 2002, 4492, 5807, 7676, 7709, 7712
BsuRI GGCC 5 cut(s) 137, 2619, 3438, 4140, 7752
BsuTUI ATCGAT 1 cut(s) 5178
BtgI CCRYGG 7 cut(s) 206, 665, 2399, 2692, 4474, 4782, 4873
BtrI CACGTC 3 cut(s) 16, 3802, 5285
BtsI GCAGTG 1 cut(s) 5679
BveI ACCTGC 2 cut(s) 2953, 6112
Cac8I GCNNGC 9 cut(s) 1115, 1410, 1714, 2367, 3217, 3554, 3648, 4142, 6667
CaiI CAGNNNCTG 1 cut(s) 164
CciI TCATGA 4 cut(s) 646, 2316, 3157, 7426
CfoI GCGC 5 cut(s) 1003, 2396, 4764, 4923, 7082
Cfr10I RCCGGY 4 cut(s) 1080, 2146, 2365, 2822
Cfr13I GGNCC 7 cut(s) 136, 1093, 3688, 4138, 7198, 7750, 7751
ClaI ATCGAT 1 cut(s) 5178
CseI GACGC 1 cut(s) 4378
CsiI ACCWGGT 1 cut(s) 6484
CspAI ACCGGT 2 cut(s) 1080, 2146
DraI TTTAAA 3 cut(s) 1600, 3021, 4795
DraIII CACNNNGTG 1 cut(s) 6686
DrdI GACNNNNNNGTC 2 cut(s) 4684, 7114
DriI GACNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 2 cut(s) 4684, 7114
Eam1104I CTCTTC 5 cut(s) 1954, 2056, 2211, 3083, 5435
Eam1105I GACNNNNNGTC 1 cut(s) 65
EarI CTCTTC 5 cut(s) 1954, 2056, 2211, 3083, 5435
EciI GGCGGA 2 cut(s) 7695, 7731
Eco105I TACGTA 1 cut(s) 7723
Eco130I CCWWGG 8 cut(s) 206, 1941, 2278, 2399, 3721, 4873, 6557, 7392
Eco24I GRGCYC 2 cut(s) 1949, 7754
Eco31I GGTCTC 3 cut(s) 802, 5334, 6286
Eco32I GATATC 2 cut(s) 456, 4333
Eco47I GGWCC 3 cut(s) 1093, 3688, 7198
Eco47III AGCGCT 1 cut(s) 1002
Eco72I CACGTG 1 cut(s) 6683
Eco88I CYCGRG 2 cut(s) 1462, 4962
Eco91I GGTNACC 1 cut(s) 2149
EcoNI CCTNNNNNAGG 1 cut(s) 6306
EcoO109I RGGNCCY 2 cut(s) 136, 7750
EcoO65I GGTNACC 1 cut(s) 2149
EcoRI GAATTC 2 cut(s) 3392, 4102
EcoRII CCWGG 7 cut(s) 502, 3438, 4097, 5809, 6484, 6532, 7506
EcoRV GATATC 2 cut(s) 456, 4333
EcoT14I CCWWGG 8 cut(s) 206, 1941, 2278, 2399, 3721, 4873, 6557, 7392
EcoT22I ATGCAT 1 cut(s) 6509
EcoT38I GRGCYC 2 cut(s) 1949, 7754
ErhI CCWWGG 8 cut(s) 206, 1941, 2278, 2399, 3721, 4873, 6557, 7392
Esp3I CGTCTC 2 cut(s) 3053, 5589
FaqI GGGAC 6 cut(s) 1106, 2515, 4331, 4892, 6266, 7120
FauNDI CATATG 5 cut(s) 2970, 5191, 5599, 5686, 6997
FbaI TGATCA 2 cut(s) 2215, 4585
FblI GTMKAC 4 cut(s) 2569, 5149, 5345, 6224
FriOI GRGCYC 2 cut(s) 1949, 7754
GlaI GCGC 5 cut(s) 1002, 2395, 4763, 4922, 7081
GsaI CCCAGC 2 cut(s) 3560, 7757
GsuI CTGGAG 8 cut(s) 1283, 1713, 2539, 2681, 4164, 6499, 6658, 6667
HaeII RGCGCY 2 cut(s) 1004, 7083
HaeIII GGCC 5 cut(s) 137, 2619, 3438, 4140, 7752
HapII CCGG 9 cut(s) 1081, 1489, 1655, 2147, 2156, 2366, 2823, 3114, 4315
HgaI GACGC 1 cut(s) 4378
HhaI GCGC 5 cut(s) 1003, 2396, 4764, 4923, 7082
Hin6I GCGC 5 cut(s) 1001, 2394, 4762, 4921, 7080
HinP1I GCGC 5 cut(s) 1001, 2394, 4762, 4921, 7080
HincII GTYRAC 5 cut(s) 1590, 2167, 4528, 5398, 6574
HindII GTYRAC 5 cut(s) 1590, 2167, 4528, 5398, 6574
HindIII AAGCTT 7 cut(s) 72, 2748, 3008, 4457, 5609, 6456, 7170
HpaI GTTAAC 1 cut(s) 6574
HpaII CCGG 9 cut(s) 1081, 1489, 1655, 2147, 2156, 2366, 2823, 3114, 4315
Hpy99I CGWCG 3 cut(s) 1125, 3655, 4262
HspAI GCGC 5 cut(s) 1001, 2394, 4762, 4921, 7080
Kpn2I TCCGGA 1 cut(s) 4314
KpnI GGTACC 2 cut(s) 189, 6472
KroI GCCGGC 1 cut(s) 2365
KroNI GCCGGC 1 cut(s) 2367
Ksp22I TGATCA 2 cut(s) 2215, 4585
KspAI GTTAAC 1 cut(s) 6574
LguI GCTCTTC 2 cut(s) 1954, 2211
LmnI GCTCC 8 cut(s) 1302, 1918, 2761, 4043, 4839, 6484, 6639, 6952
MabI ACCWGGT 1 cut(s) 6484
MfeI CAATTG 3 cut(s) 2787, 4934, 6474
MhlI GDGCHC 7 cut(s) 598, 1849, 1949, 2289, 6166, 6600, 7754
MmeI TCCRAC 4 cut(s) 1714, 3113, 4578, 6921
Mph1103I ATGCAT 1 cut(s) 6509
MroI TCCGGA 1 cut(s) 4314
MroNI GCCGGC 1 cut(s) 2365
MroXI GAANNNNTTC 2 cut(s) 1745, 5610
MslI CAYNNNNRTG 9 cut(s) 1027, 1324, 3348, 3365, 3377, 3599, 4209, 5220, 7323
MspA1I CMGCKG 2 cut(s) 2840, 6359
MspCI CTTAAG 3 cut(s) 2999, 3445, 4055
MspI CCGG 9 cut(s) 1081, 1489, 1655, 2147, 2156, 2366, 2823, 3114, 4315
MspR9I CCNGG 9 cut(s) 504, 2156, 3114, 3440, 4099, 5811, 6486, 6534, 7508
MssI GTTTAAAC 1 cut(s) 1600
MunI CAATTG 3 cut(s) 2787, 4934, 6474
Mva1269I GAATGC 2 cut(s) 5880, 6898
MvaI CCWGG 7 cut(s) 504, 3440, 4099, 5811, 6486, 6534, 7508
MvnI CGCG 1 cut(s) 2394
NaeI GCCGGC 1 cut(s) 2367
NciI CCSGG 2 cut(s) 2156, 3114
NcoI CCATGG 3 cut(s) 206, 2399, 4873
NdeI CATATG 5 cut(s) 2970, 5191, 5599, 5686, 6997
NgoMIV GCCGGC 1 cut(s) 2365
NheI GCTAGC 1 cut(s) 1712
NsiI ATGCAT 1 cut(s) 6509
NspI RCATGY 4 cut(s) 1250, 1477, 2038, 6669
NspV TTCGAA 2 cut(s) 6, 7574
PaeI GCATGC 1 cut(s) 6669
PaeR7I CTCGAG 1 cut(s) 4962
PagI TCATGA 4 cut(s) 646, 2316, 3157, 7426
PciI ACATGT 1 cut(s) 2034
PciSI GCTCTTC 2 cut(s) 1954, 2211
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PctI GAATGC 2 cut(s) 5880, 6898
PdiI GCCGGC 1 cut(s) 2367
PdmI GAANNNNTTC 2 cut(s) 1745, 5610
PflFI GACNNNGTC 3 cut(s) 2846, 7207, 7331
PflMI CCANNNNNTGG 2 cut(s) 760, 6362
PfoI TCCNGGA 1 cut(s) 502
PinAI ACCGGT 2 cut(s) 1080, 2146
PmaCI CACGTG 1 cut(s) 6683
PmeI GTTTAAAC 1 cut(s) 1600
PmlI CACGTG 1 cut(s) 6683
Ppu21I YACGTR 4 cut(s) 737, 2472, 6683, 7723
PscI ACATGT 1 cut(s) 2034
PshBI ATTAAT 3 cut(s) 1416, 2853, 5772
PsiI TTATAA 2 cut(s) 2054, 5276
Psp6I CCWGG 7 cut(s) 502, 3438, 4097, 5809, 6484, 6532, 7506
PspCI CACGTG 1 cut(s) 6683
PspEI GGTNACC 1 cut(s) 2149
PspFI CCCAGC 2 cut(s) 3556, 7753
PspGI CCWGG 7 cut(s) 502, 3438, 4097, 5809, 6484, 6532, 7506
PspOMI GGGCCC 1 cut(s) 7750
PspPI GGNCC 7 cut(s) 136, 1093, 3688, 4138, 7198, 7750, 7751
PspXI VCTCGAGB 1 cut(s) 4962
PsrI GAACNNNNNNTAC 4 cut(s) 2568, 2590, 2600, 2622
PstI CTGCAG 4 cut(s) 2413, 3478, 4201, 7606
PstNI CAGNNNCTG 1 cut(s) 164
PsyI GACNNNGTC 3 cut(s) 2846, 7207, 7331
PvuII CAGCTG 2 cut(s) 2840, 6359
RseI CAYNNNNRTG 9 cut(s) 1027, 1324, 3348, 3365, 3377, 3599, 4209, 5220, 7323
SapI GCTCTTC 2 cut(s) 1954, 2211
Sau96I GGNCC 7 cut(s) 136, 1093, 3688, 4138, 7198, 7750, 7751
ScaI AGTACT 3 cut(s) 3472, 6900, 7811
ScrFI CCNGG 9 cut(s) 504, 2156, 3114, 3440, 4099, 5811, 6486, 6534, 7508
SduI GDGCHC 7 cut(s) 598, 1849, 1949, 2289, 6166, 6600, 7754
SexAI ACCWGGT 1 cut(s) 6484
SfcI CTRYAG 9 cut(s) 2409, 2463, 3474, 4197, 4248, 4912, 5799, 5832, 7602
Sfr274I CTCGAG 1 cut(s) 4962
SfuI TTCGAA 2 cut(s) 6, 7574
SinI GGWCC 3 cut(s) 1093, 3688, 7198
SlaI CTCGAG 1 cut(s) 4962
SmiMI CAYNNNNRTG 9 cut(s) 1027, 1324, 3348, 3365, 3377, 3599, 4209, 5220, 7323
SmlI CTYRAG 9 cut(s) 293, 1047, 2939, 2999, 3445, 4055, 4962, 6073, 6590
SmoI CTYRAG 9 cut(s) 293, 1047, 2939, 2999, 3445, 4055, 4962, 6073, 6590
SnaBI TACGTA 1 cut(s) 7723
SphI GCATGC 1 cut(s) 6669
SspI AATATT 7 cut(s) 926, 2509, 5482, 5842, 6372, 6586, 6981
StyD4I CCNGG 9 cut(s) 502, 2154, 3112, 3438, 4097, 5809, 6484, 6532, 7506
StyI CCWWGG 8 cut(s) 206, 1941, 2278, 2399, 3721, 4873, 6557, 7392
TaqII GACCGA 1 cut(s) 481
TauI GCSGC 4 cut(s) 1283, 4052, 6958, 7085
TspGWI ACGGA 8 cut(s) 2099, 2681, 3194, 3365, 3728, 4463, 4799, 5606
Tth111I GACNNNGTC 3 cut(s) 2846, 7207, 7331
Van91I CCANNNNNTGG 2 cut(s) 760, 6362
Vha464I CTTAAG 3 cut(s) 2999, 3445, 4055
VneI GTGCAC 2 cut(s) 2285, 6162
VpaK11BI GGWCC 3 cut(s) 1093, 3688, 7198
VspI ATTAAT 3 cut(s) 1416, 2853, 5772
XagI CCTNNNNNAGG 1 cut(s) 6306
XbaI TCTAGA 2 cut(s) 5446, 6802
XceI RCATGY 4 cut(s) 1250, 1477, 2038, 6669
XcmI CCANNNNNNNNNTGG 4 cut(s) 1029, 6285, 7319, 7709
XhoI CTCGAG 1 cut(s) 4962
XmaJI CCTAGG 2 cut(s) 3721, 6557
XmiI GTMKAC 4 cut(s) 2569, 5149, 5345, 6224
XmnI GAANNNNTTC 2 cut(s) 1745, 5610
ZrmI AGTACT 3 cut(s) 3472, 6900, 7811
Zsp2I ATGCAT 1 cut(s) 6509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.