RchiOBHm_Chr4g0407731

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0002758
Physical Location & Seq
Forward (+)
30065664 .. 30066439
776 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37895

Sequence Viewer

Length: 687 bp
ATGTATGTTTTACTTCATGAGGAGGATGATACTTTTACAGATGCTTTGCCAGATTTTATGTCTGTATCTGATGCAGGCTTTGGTTCACCAACTTCAGATACGGTTTCATGTCTAACAACTGAGGACAGCAATGATGCTGCTGGATTTGCATCTGCTAAAGGCTTAGTCCTTGAAAAGAATCTTGTTACAGCAAGGTGCATTTCTGCTGAGGAATTTTATGAGGCGGAGGGTAGTGACTATTCCAATTTTGTCTTTCTAACCTTCTCGACTCTGAGTTCTAGTTCACCTGATTATGATGGTATTGACACACAGTTGAGCATCCGTATGTCAAAACTGGAATTCTTCTGCAACAGACCTACTTTGGTCGCTTTAATTGATTTTGGCCTGGACTTAAGCTCTGTGTACAGTGAGGAAAACACTGCAGACATTACTGAAGGTTCAGGTGACAAATCTTTGACGAACAAAGAAAAGACCGAAGAAATTGGGCGTGTCAAAGGCTTGTTGGGTTATGGTAAAGGTCGTGTAGTATTTTATTTGAACATGAACGCGTACGTGGACAGTGTGACTGTGTTTCTCAACAAGGAGGATGCTTCTCTGCTTGCGACGTTTGTGCAAGAAAGTTTTGTGCTAGATCTGAAGGTGAGGAAGTTCTGTAATAGAAGTATGAATTTGCTTGAAATTCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

228

Amino Acids

25.09

Weight (kDa)

4.28

Isoelectric Point (pI)

40.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 548
AciI CCGC 1 cut(s) 224
AcsI RAATTY 4 cut(s) 212, 338, 667, 678
AcuI CTGAAG 3 cut(s) 78, 453, 656
AfaI GTAC 2 cut(s) 404, 551
AflII CTTAAG 1 cut(s) 391
AflIII ACRYGT 1 cut(s) 546
AgsI TTSAA 3 cut(s) 173, 538, 677
AjnI CCWGG 1 cut(s) 384
AluBI AGCT 1 cut(s) 396
AluI AGCT 1 cut(s) 396
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 137
ApoI RAATTY 4 cut(s) 212, 338, 667, 678
AsuHPI GGTGA 4 cut(s) 78, 276, 455, 652
BbvCI CCTCAGC 1 cut(s) 207
BbvI GCAGC 1 cut(s) 124
BccI CCATC 1 cut(s) 290
BcgI CGANNNNNNTGC 2 cut(s) 592, 626
BciT130I CCWGG 1 cut(s) 386
BfaI CTAG 2 cut(s) 279, 629
BfmI CTRYAG 1 cut(s) 420
BfrI CTTAAG 1 cut(s) 391
BglII AGATCT 1 cut(s) 631
BisI GCNGC 1 cut(s) 138
BlsI GCNGC 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 386
BmrFI CCNGG 1 cut(s) 386
BmsI GCATC 6 cut(s) 31, 61, 124, 158, 327, 577
Bpu10I CCTNAGC 1 cut(s) 207
BsaAI YACGTR 1 cut(s) 553
BsaBI GATNNNNATC 1 cut(s) 148
Bse1I ACTGG 1 cut(s) 339
Bse3DI GCAATG 1 cut(s) 136
Bse8I GATNNNNATC 1 cut(s) 148
BseBI CCWGG 1 cut(s) 386
BseGI GGATG 3 cut(s) 31, 318, 592
BseJI GATNNNNATC 1 cut(s) 148
BseMI GCAATG 1 cut(s) 136
BseMII CTCAG 3 cut(s) 111, 198, 263
BseNI ACTGG 1 cut(s) 339
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 1 cut(s) 124
Bsh1236I CGCG 1 cut(s) 548
BshFI GGCC 1 cut(s) 384
BsiWI CGTACG 1 cut(s) 549
BsnI GGCC 1 cut(s) 384
Bsp1407I TGTACA 1 cut(s) 402
Bsp143I GATC 1 cut(s) 631
BspACI CCGC 1 cut(s) 224
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 3 cut(s) 112, 199, 264
BspFNI CGCG 1 cut(s) 548
BspHI TCATGA 1 cut(s) 16
BspMAI CTGCAG 1 cut(s) 424
BspTI CTTAAG 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 136
BsrGI TGTACA 1 cut(s) 402
BsrI ACTGG 1 cut(s) 339
BssMI GATC 1 cut(s) 631
Bst2UI CCWGG 1 cut(s) 386
Bst4CI ACNGT 5 cut(s) 103, 312, 407, 560, 568
BstAFI CTTAAG 1 cut(s) 391
BstAUI TGTACA 1 cut(s) 402
BstBAI YACGTR 1 cut(s) 553
BstC8I GCNNGC 2 cut(s) 76, 600
BstDEI CTNAG 4 cut(s) 120, 163, 207, 272
BstF5I GGATG 3 cut(s) 31, 318, 592
BstFNI CGCG 1 cut(s) 548
BstKTI GATC 1 cut(s) 634
BstMBI GATC 1 cut(s) 631
BstMWI GCNNNNNNNGC 1 cut(s) 146
BstNI CCWGG 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 384
BstSFI CTRYAG 1 cut(s) 420
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 1 cut(s) 124
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 1 cut(s) 384
BtsCI GGATG 3 cut(s) 31, 318, 592
BtsI GCAGTG 1 cut(s) 417
BtsIMutI CAGTG 3 cut(s) 412, 417, 565
Cac8I GCNNGC 2 cut(s) 76, 600
CciI TCATGA 1 cut(s) 16
Csp6I GTAC 2 cut(s) 403, 550
CviAII CATG 3 cut(s) 17, 108, 541
CviJI RGCY 5 cut(s) 78, 162, 384, 396, 498
CviKI_1 RGCY 5 cut(s) 78, 162, 384, 396, 498
CviQI GTAC 2 cut(s) 403, 550
DdeI CTNAG 4 cut(s) 120, 163, 207, 272
DpnI GATC 1 cut(s) 633
DpnII GATC 1 cut(s) 631
EciI GGCGGA 1 cut(s) 239
Eco57I CTGAAG 3 cut(s) 78, 453, 656
EcoRI GAATTC 1 cut(s) 338
EcoRII CCWGG 1 cut(s) 384
FaeI CATG 3 cut(s) 20, 111, 544
FatI CATG 3 cut(s) 16, 107, 540
Fnu4HI GCNGC 1 cut(s) 138
FokI GGATG 3 cut(s) 38, 305, 599
Fsp4HI GCNGC 1 cut(s) 138
FspBI CTAG 2 cut(s) 279, 629
GluI GCNGC 1 cut(s) 138
HaeIII GGCC 1 cut(s) 384
Hin1II CATG 3 cut(s) 20, 111, 544
HinfI GANTC 2 cut(s) 178, 268
HphI GGTGA 4 cut(s) 78, 276, 455, 652
Hpy166II GTNNAC 4 cut(s) 86, 284, 403, 556
Hpy188I TCNGA 4 cut(s) 70, 97, 273, 636
Hpy188III TCNNGA 2 cut(s) 17, 265
Hpy8I GTNNAC 4 cut(s) 86, 284, 403, 556
Hpy99I CGWCG 1 cut(s) 607
HpyAV CCTTC 3 cut(s) 271, 428, 631
HpyCH4III ACNGT 5 cut(s) 103, 312, 407, 560, 568
HpyCH4IV ACGT 2 cut(s) 552, 605
HpyCH4V TGCA 6 cut(s) 74, 149, 198, 348, 422, 613
HpyF10VI GCNNNNNNNGC 1 cut(s) 146
HpyF3I CTNAG 4 cut(s) 120, 163, 207, 272
HpySE526I ACGT 2 cut(s) 552, 605
Hsp92II CATG 3 cut(s) 20, 111, 544
Kzo9I GATC 1 cut(s) 631
LpnPI CCDG 8 cut(s) 60, 63, 126, 300, 320, 371, 398, 426
Lsp1109I GCAGC 1 cut(s) 124
LweI GCATC 6 cut(s) 31, 61, 124, 158, 327, 577
MaeI CTAG 2 cut(s) 279, 629
MaeII ACGT 2 cut(s) 552, 605
MaeIII GTNAC 4 cut(s) 184, 233, 443, 562
MalI GATC 1 cut(s) 633
MboI GATC 1 cut(s) 631
MboII GAAGA 2 cut(s) 334, 488
MflI RGATCY 1 cut(s) 631
MluCI AATT 7 cut(s) 212, 244, 338, 372, 480, 667, 678
MluI ACGCGT 1 cut(s) 546
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 9 cut(s) 13, 16, 115, 202, 214, 220, 403, 577, 636
MseI TTAA 2 cut(s) 371, 392
MslI CAYNNNNRTG 1 cut(s) 323
MspCI CTTAAG 1 cut(s) 391
MspR9I CCNGG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 386
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 1 cut(s) 146
NdeII GATC 1 cut(s) 631
NlaIII CATG 3 cut(s) 20, 111, 544
NmuCI GTSAC 3 cut(s) 233, 443, 562
PagI TCATGA 1 cut(s) 16
PfeI GAWTC 1 cut(s) 178
Pfl23II CGTACG 1 cut(s) 549
PkrI GCNGC 1 cut(s) 139
PleI GAGTC 1 cut(s) 262
PpsI GAGTC 1 cut(s) 262
Ppu21I YACGTR 1 cut(s) 553
Psp6I CCWGG 1 cut(s) 384
PspGI CCWGG 1 cut(s) 384
PspLI CGTACG 1 cut(s) 549
PstI CTGCAG 1 cut(s) 424
PsuI RGATCY 1 cut(s) 631
RsaI GTAC 2 cut(s) 404, 551
RsaNI GTAC 2 cut(s) 403, 550
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 2 cut(s) 371, 392
SatI GCNGC 1 cut(s) 138
Sau3AI GATC 1 cut(s) 631
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 386
SfaNI GCATC 6 cut(s) 31, 61, 124, 158, 327, 577
SfcI CTRYAG 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 7 cut(s) 212, 244, 338, 372, 480, 667, 678
SsiI CCGC 1 cut(s) 224
SspMI CTAG 2 cut(s) 279, 629
StyD4I CCNGG 1 cut(s) 384
TaaI ACNGT 5 cut(s) 103, 312, 407, 560, 568
TaiI ACGT 2 cut(s) 555, 608
TaqI TCGA 1 cut(s) 266
TaqII GACCGA 1 cut(s) 488
TasI AATT 7 cut(s) 212, 244, 338, 372, 480, 667, 678
TatI WGTACW 1 cut(s) 402
TfiI GAWTC 1 cut(s) 178
Tru1I TTAA 2 cut(s) 371, 392
Tru9I TTAA 2 cut(s) 371, 392
TscAI CASTG 3 cut(s) 412, 424, 565
TseFI GTSAC 3 cut(s) 233, 443, 562
TseI GCWGC 1 cut(s) 137
Tsp45I GTSAC 3 cut(s) 233, 443, 562
TspDTI ATGAA 4 cut(s) 5, 96, 557, 680
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 3 cut(s) 412, 424, 565
Vha464I CTTAAG 1 cut(s) 391
XapI RAATTY 4 cut(s) 212, 338, 667, 678
XspI CTAG 2 cut(s) 279, 629
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.