Rorug04G0092700

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002758
Physical Location & Seq
Forward (+)
14322728 .. 14324416
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0092700.1

Sequence Viewer

Length: 552 bp
ATGGACACCGTCGTCTCCAATTCAGCATTACTATCCATACCACCCTTCTCTCCAGTCCGTACAGTTACTTGCAACTCTTATTCACCAACCACTTCTCTCAATCTCTCGTCAAAGAAGCACTTCTCCAGCTTCTTCTACACCCAAAAGAAACAAGTTGGTCATCGCAACTCTTCTTGTACAGCTCCCAAGTTTTCAATCGCTTCAAGTGCTGCTATTCAGGAAATCAACGAGACCCAGTTTCGGGATACGGTTCTTAACTCGGACCGTCCGGTTCTTGTCGAGTTTGTTGCCAACTGGTGCGGTCCTTGCCGCTTAATTTCTCCCGCCATGGAATGGGTTGCTCAGGAATATAAAGACAGATTAGCAGTTGTTAAGATCGATCATGATGCAAACCCACGATTAATTGAAGAATACAAAGTTTATGGTTTGCCTGCTTTGATTCTCTTCAAGAATGGACAGGAAGTTCCAGAAAGTAGAAGAGAGGGTGCAATTACAAAAGTTAAGCTTAAAGAGTATGTGGATGCTTTTTTGGAATCTATATCAGTCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

183

Amino Acids

20.46

Weight (kDa)

8.32

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 73 - 174 1.7e-27 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AccB7I CCANNNNNTGG 1 cut(s) 333
AciI CCGC 3 cut(s) 300, 310, 324
AfaI GTAC 2 cut(s) 61, 178
AfiI CCNNNNNNNGG 3 cut(s) 240, 241, 333
AgsI TTSAA 4 cut(s) 195, 204, 407, 448
AloI GAACNNNNNNTCC 2 cut(s) 447, 479
AluBI AGCT 3 cut(s) 129, 182, 505
AluI AGCT 3 cut(s) 129, 182, 505
Alw26I GTCTC 2 cut(s) 19, 224
ApeKI GCWGC 1 cut(s) 209
AseI ATTAAT 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 262, 302
AsuHPI GGTGA 1 cut(s) 75
AvaII GGWCC 2 cut(s) 262, 302
BbvI GCAGC 1 cut(s) 196
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 368, 402
BciVI GTATCC 1 cut(s) 238
BcoDI GTCTC 2 cut(s) 19, 224
BfuI GTATCC 1 cut(s) 238
BisI GCNGC 2 cut(s) 210, 310
BlsI GCNGC 2 cut(s) 211, 311
Bme18I GGWCC 2 cut(s) 262, 302
BmgT120I GGNCC 2 cut(s) 262, 302
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 2 cut(s) 376, 511
BmuI ACTGGG 1 cut(s) 229
BpmI CTGGAG 2 cut(s) 36, 109
Bpu10I CCTNAGC 1 cut(s) 342
Bsa29I ATCGAT 1 cut(s) 378
BsaI GGTCTC 1 cut(s) 224
BsaJI CCNNGG 1 cut(s) 327
BsaWI WCCGGW 1 cut(s) 268
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 241, 333
Bse1I ACTGG 3 cut(s) 53, 235, 299
BseCI ATCGAT 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 327
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 3 cut(s) 240, 241, 333
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 3 cut(s) 53, 235, 299
BseXI GCAGC 1 cut(s) 196
BshVI ATCGAT 1 cut(s) 378
BsiSI CCGG 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 240, 241, 333
BsmAI GTCTC 2 cut(s) 19, 224
BsmBI CGTCTC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 224
Bsp1407I TGTACA 1 cut(s) 176
Bsp143I GATC 2 cut(s) 375, 379
Bsp19I CCATGG 1 cut(s) 327
BspACI CCGC 3 cut(s) 300, 310, 324
BspCNI CTCAG 1 cut(s) 355
BspDI ATCGAT 1 cut(s) 378
BspHI TCATGA 1 cut(s) 382
BspTNI GGTCTC 1 cut(s) 224
BsrGI TGTACA 1 cut(s) 176
BsrI ACTGG 3 cut(s) 53, 235, 299
BssECI CCNNGG 1 cut(s) 327
BssMI GATC 2 cut(s) 375, 379
BssT1I CCWWGG 1 cut(s) 327
Bst4CI ACNGT 4 cut(s) 10, 64, 250, 266
Bst6I CTCTTC 3 cut(s) 175, 449, 472
BstAUI TGTACA 1 cut(s) 176
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 327
BstF5I GGATG 1 cut(s) 526
BstKTI GATC 2 cut(s) 378, 382
BstMAI GTCTC 2 cut(s) 19, 224
BstMBI GATC 2 cut(s) 375, 379
BstMWI GCNNNNNNNGC 2 cut(s) 206, 306
BstV1I GCAGC 1 cut(s) 196
Bsu15I ATCGAT 1 cut(s) 378
BsuI GTATCC 1 cut(s) 238
BsuTUI ATCGAT 1 cut(s) 378
BtgI CCRYGG 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 146
BtsCI GGATG 1 cut(s) 526
Cac8I GCNNGC 1 cut(s) 432
CciI TCATGA 1 cut(s) 382
Cfr13I GGNCC 2 cut(s) 262, 302
ClaI ATCGAT 1 cut(s) 378
CpoI CGGWCCG 1 cut(s) 262
Csp6I GTAC 2 cut(s) 60, 177
CspI CGGWCCG 1 cut(s) 262
CviAII CATG 2 cut(s) 328, 383
CviJI RGCY 3 cut(s) 129, 182, 505
CviKI_1 RGCY 3 cut(s) 129, 182, 505
CviQI GTAC 2 cut(s) 60, 177
DdeI CTNAG 1 cut(s) 342
DpnI GATC 2 cut(s) 377, 381
DpnII GATC 2 cut(s) 375, 379
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Eam1104I CTCTTC 3 cut(s) 175, 449, 472
EarI CTCTTC 3 cut(s) 175, 449, 472
Eco130I CCWWGG 1 cut(s) 327
Eco31I GGTCTC 1 cut(s) 224
Eco47I GGWCC 2 cut(s) 262, 302
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 1 cut(s) 19
FaeI CATG 2 cut(s) 331, 386
FaiI YATR 8 cut(s) 38, 329, 351, 384, 423, 516, 539, 550
FalI AAGNNNNNCTT 4 cut(s) 104, 136, 489, 521
FatI CATG 2 cut(s) 327, 382
FauI CCCGC 1 cut(s) 331
Fnu4HI GCNGC 2 cut(s) 210, 310
FokI GGATG 1 cut(s) 533
Fsp4HI GCNGC 2 cut(s) 210, 310
GluI GCNGC 2 cut(s) 210, 310
GsuI CTGGAG 2 cut(s) 36, 109
HapII CCGG 1 cut(s) 269
Hin1II CATG 2 cut(s) 331, 386
HindIII AAGCTT 1 cut(s) 503
HinfI GANTC 2 cut(s) 439, 533
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 75
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 6 cut(s) 218, 242, 344, 383, 448, 467
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 4 cut(s) 10, 64, 250, 266
HpyCH4V TGCA 3 cut(s) 72, 389, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 206, 306
HpyF3I CTNAG 1 cut(s) 342
Hsp92II CATG 2 cut(s) 331, 386
Kzo9I GATC 2 cut(s) 375, 379
LmnI GCTCC 1 cut(s) 187
Lsp1109I GCAGC 1 cut(s) 196
LweI GCATC 2 cut(s) 376, 511
MaeIII GTNAC 1 cut(s) 64
MalI GATC 2 cut(s) 377, 381
MboI GATC 2 cut(s) 375, 379
MboII GAAGA 5 cut(s) 124, 162, 419, 436, 489
MluCI AATT 4 cut(s) 19, 315, 402, 489
MnlI CCTC 1 cut(s) 475
MroXI GAANNNNTTC 1 cut(s) 119
MseI TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
MspI CCGG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 206, 306
NcoI CCATGG 1 cut(s) 327
NdeII GATC 2 cut(s) 375, 379
NlaIII CATG 2 cut(s) 331, 386
PagI TCATGA 1 cut(s) 382
PdmI GAANNNNTTC 1 cut(s) 119
PfeI GAWTC 2 cut(s) 439, 533
PflFI GACNNNGTC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 2 cut(s) 211, 311
PshBI ATTAAT 1 cut(s) 401
PspPI GGNCC 2 cut(s) 262, 302
PsyI GACNNNGTC 1 cut(s) 8
RsaI GTAC 2 cut(s) 61, 178
RsaNI GTAC 2 cut(s) 60, 177
Rsr2I CGGWCCG 1 cut(s) 262
RsrII CGGWCCG 1 cut(s) 262
SaqAI TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
SatI GCNGC 2 cut(s) 210, 310
Sau3AI GATC 2 cut(s) 375, 379
Sau96I GGNCC 2 cut(s) 262, 302
SetI ASST 3 cut(s) 131, 184, 507
SfaNI GCATC 2 cut(s) 376, 511
SinI GGWCC 2 cut(s) 262, 302
Sse9I AATT 4 cut(s) 19, 315, 402, 489
SsiI CCGC 3 cut(s) 300, 310, 324
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 4 cut(s) 10, 64, 250, 266
TaqI TCGA 2 cut(s) 279, 378
TasI AATT 4 cut(s) 19, 315, 402, 489
TatI WGTACW 1 cut(s) 176
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 2 cut(s) 439, 533
Tru1I TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
Tru9I TTAA 6 cut(s) 255, 314, 372, 401, 501, 507
TseI GCWGC 1 cut(s) 209
TspGWI ACGGA 1 cut(s) 47
Tth111I GACNNNGTC 1 cut(s) 8
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 2 cut(s) 262, 302
VspI ATTAAT 1 cut(s) 401
XmnI GAANNNNTTC 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.