Rorug04G0212200

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0002758
Physical Location & Seq
Forward (+)
36882792 .. 36888507
5716 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0212200.1

Sequence Viewer

Length: 1191 bp
ATGGCAGCATCAACTTCTTGTGCCAGTAAGAGCCCATTTCCCATGAAAGACACAACCTCTAACTTAATTCCATACCCAGCACCTCTAGCTAAATATGAGGAGGTTGCAGCGAATCCTAAGCTGTTCATGTCTACTTTGGAAAAGCTCCATACTTCAATGGGAACCAAGTTTATGATCCCTATAATAGGAGGAAGAGAGTTAGACTTGCATCGACTGTTTGTGGAGGTAACTTCCCGTGGTGGTATGGCAAAGATTGTTAGAGATAGAAGATGGAAGGAAGTTACCGCTATATTCAACTTCCCCTCCACAGCTACAAATGCTTCTTTTGTGCTGCGGAAATATTATAATTCGTTGCTTCTCCACTATGAACAAATATACTATTTCAAAGCTCAAGCATGGAATCCTCTCTCTTCTGATATTTCACAGAACCATAACATGACACCTGGTCCAGCTCCAAGGGGAGGAACTAAACGAAAATCAACTGAAGCTCAGACCCTTCTGCTTCAGCAACCACAGATAATTCCAGAGATTCCTGGAGCCGGGACACCAGCACCGTCAGAAGATGACACTGTGTTTGGGTTTATAGATGGGAAATTCGAAAGCGGATATCTTGTTACCGTCACCAAAGGCACAGAGAAGTTAAGTGGAGTACTGTACCAAGTTCCACAGAATCCAGTACAGGAGGTTCCACAAGGCCCACAGAATTATAGCATGGTGGTCAACAGAAACGACAGTACCTCAGCTGTACCTGTTCCCCATCGTCGTCGACGAAGAAAGAAATCTGAGATAAAAAGGAGGGACCCTGCTCACCCAAAACCGAATAGAAGTGGGTATAACTTTTTCTTTGCTGAACAGCATGCAAGGCTGAAGCCACTTCATCCGGGGAAGGATAGAGAGATAAGTAGAATGATTGGTGAATTGTGGAATAAATTGAAGGAGTCTGATAAAGGAGTGTATCAGGAGAAAGCCGTGAAAGATAAAGAGAGGTACCGAGTAGAAATGGAGGAATACCGGGAGAGGTTGAAGGCTGCTCAAGTCATCAGTGATGCAGTCCCACTACAGCAGCGGTCTCCTGAAGCAGATGCAAGTATGGCAGAATCAGAAACCAAGATTGAAGAAACCGAGCCTGGCGACTCTCCTGAAACTGCAGATGAAAGTAGCGAGAGTAGTTCTAGCAAATCAGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

396

Amino Acids

44.57

Weight (kDa)

9.13

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARID PF01388 39 - 123 5.3e-22 ARID/BRIGHT DNA binding domain
HMG_box_2 PF09011 268 - 337 2.2e-10 HMG-box domain
HMG_box PF00505 271 - 338 2.7e-15 HMG (high mobility group) box
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 345
Acc65I GGTACC 1 cut(s) 987
AccB1I GGYRCC 1 cut(s) 987
AccI GTMKAC 2 cut(s) 131, 766
AciI CCGC 4 cut(s) 285, 334, 603, 1066
AclWI GGATC 1 cut(s) 169
AcsI RAATTY 1 cut(s) 593
AcuI CTGAAG 4 cut(s) 488, 504, 887, 1095
AfaI GTAC 6 cut(s) 651, 656, 678, 736, 747, 989
AfiI CCNNNNNNNGG 3 cut(s) 185, 461, 539
AgsI TTSAA 6 cut(s) 156, 295, 385, 934, 1024, 1115
AhdI GACNNNNNGTC 1 cut(s) 444
AjnI CCWGG 3 cut(s) 442, 532, 1126
AluBI AGCT 8 cut(s) 89, 121, 145, 311, 389, 452, 488, 743
AluI AGCT 8 cut(s) 89, 121, 145, 311, 389, 452, 488, 743
Alw26I GTCTC 1 cut(s) 1074
AlwI GGATC 1 cut(s) 169
AoxI GGCC 1 cut(s) 694
ApeKI GCWGC 5 cut(s) 5, 107, 331, 1028, 1063
ApoI RAATTY 1 cut(s) 593
ArsI GACNNNNNNTTYG 2 cut(s) 557, 589
Asp718I GGTACC 1 cut(s) 987
AspS9I GGNCC 3 cut(s) 446, 695, 799
AsuC2I CCSGG 3 cut(s) 541, 882, 1013
AsuHPI GGTGA 3 cut(s) 613, 800, 926
AsuII TTCGAA 1 cut(s) 597
AvaII GGWCC 2 cut(s) 446, 799
BaeI ACNNNNGTAYC 2 cut(s) 718, 751
BanI GGYRCC 1 cut(s) 987
BanII GRGCYC 1 cut(s) 35
BbvCI CCTCAGC 1 cut(s) 739
BbvI GCAGC 5 cut(s) 17, 119, 318, 1015, 1075
BccI CCATC 3 cut(s) 264, 581, 765
BceAI ACGGC 1 cut(s) 953
BciT130I CCWGG 3 cut(s) 444, 534, 1128
BcnI CCSGG 3 cut(s) 541, 882, 1013
BcoDI GTCTC 1 cut(s) 1074
BfaI CTAG 2 cut(s) 86, 1173
BfmI CTRYAG 2 cut(s) 1058, 1146
BisI GCNGC 5 cut(s) 6, 108, 332, 1029, 1064
BlsI GCNGC 5 cut(s) 7, 109, 333, 1030, 1065
BmcAI AGTACT 1 cut(s) 651
Bme1390I CCNGG 6 cut(s) 444, 534, 541, 882, 1013, 1128
Bme18I GGWCC 2 cut(s) 446, 799
BmeRI GACNNNNNGTC 1 cut(s) 444
BmgT120I GGNCC 3 cut(s) 446, 695, 799
BmiI GGNNCC 6 cut(s) 163, 538, 687, 800, 801, 989
BmrFI CCNGG 6 cut(s) 444, 534, 541, 882, 1013, 1128
BmsI GCATC 4 cut(s) 17, 217, 1036, 1072
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 2 cut(s) 117, 739
Bpu14I TTCGAA 1 cut(s) 597
BpuEI CTTGAG 2 cut(s) 375, 1017
BpuMI CCSGG 3 cut(s) 541, 882, 1013
BsaI GGTCTC 1 cut(s) 1074
BsaJI CCNNGG 3 cut(s) 235, 455, 881
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 461, 539
Bse1I ACTGG 2 cut(s) 24, 674
BseBI CCWGG 3 cut(s) 444, 534, 1128
BseDI CCNNGG 3 cut(s) 235, 455, 881
BseGI GGATG 1 cut(s) 877
BseLI CCNNNNNNNGG 3 cut(s) 185, 461, 539
BseMII CTCAG 3 cut(s) 503, 753, 774
BseNI ACTGG 2 cut(s) 24, 674
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 5 cut(s) 17, 119, 318, 1015, 1075
BseYI CCCAGC 1 cut(s) 76
BshFI GGCC 1 cut(s) 696
BshNI GGYRCC 1 cut(s) 987
BsiSI CCGG 3 cut(s) 540, 881, 1012
BslFI GGGAC 3 cut(s) 556, 812, 1037
BslI CCNNNNNNNGG 3 cut(s) 185, 461, 539
BsmAI GTCTC 1 cut(s) 1074
BsmFI GGGAC 3 cut(s) 556, 812, 1037
BsnI GGCC 1 cut(s) 696
Bso31I GGTCTC 1 cut(s) 1074
Bsp119I TTCGAA 1 cut(s) 597
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 4 cut(s) 285, 334, 603, 1066
BspANI GGCC 1 cut(s) 696
BspCNI CTCAG 3 cut(s) 502, 752, 775
BspLI GGNNCC 6 cut(s) 163, 538, 687, 800, 801, 989
BspMAI CTGCAG 1 cut(s) 1150
BspPI GGATC 1 cut(s) 169
BspT104I TTCGAA 1 cut(s) 597
BspT107I GGYRCC 1 cut(s) 987
BspTNI GGTCTC 1 cut(s) 1074
BsrI ACTGG 2 cut(s) 24, 674
BssECI CCNNGG 3 cut(s) 235, 455, 881
BssMI GATC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 455
Bst2UI CCWGG 3 cut(s) 444, 534, 1128
Bst4CI ACNGT 6 cut(s) 216, 555, 571, 619, 654, 734
Bst6I CTCTTC 2 cut(s) 187, 415
BstBI TTCGAA 1 cut(s) 597
BstC8I GCNNGC 1 cut(s) 858
BstDEI CTNAG 4 cut(s) 117, 489, 739, 783
BstDSI CCRYGG 1 cut(s) 235
BstENI CCTNNNNNAGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 877
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 1 cut(s) 1074
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 4 cut(s) 86, 317, 862, 1091
BstNI CCWGG 3 cut(s) 444, 534, 1128
BstNSI RCATGY 1 cut(s) 860
BstSCI CCNGG 6 cut(s) 442, 532, 539, 880, 1011, 1126
BstSFI CTRYAG 2 cut(s) 1058, 1146
BstV1I GCAGC 5 cut(s) 17, 119, 318, 1015, 1075
BsuRI GGCC 1 cut(s) 696
BtgI CCRYGG 1 cut(s) 235
BtsCI GGATG 1 cut(s) 877
BtsIMutI CAGTG 2 cut(s) 567, 1048
Cac8I GCNNGC 1 cut(s) 858
Cfr13I GGNCC 3 cut(s) 446, 695, 799
CsiI ACCWGGT 1 cut(s) 442
Csp6I GTAC 6 cut(s) 650, 655, 677, 735, 746, 988
CviAII CATG 6 cut(s) 43, 127, 396, 436, 712, 857
CviQI GTAC 6 cut(s) 650, 655, 677, 735, 746, 988
DdeI CTNAG 4 cut(s) 117, 489, 739, 783
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 444
Eam1104I CTCTTC 2 cut(s) 187, 415
Eam1105I GACNNNNNGTC 1 cut(s) 444
EarI CTCTTC 2 cut(s) 187, 415
Eco130I CCWWGG 1 cut(s) 455
Eco24I GRGCYC 1 cut(s) 35
Eco31I GGTCTC 1 cut(s) 1074
Eco32I GATATC 1 cut(s) 608
Eco47I GGWCC 2 cut(s) 446, 799
Eco57I CTGAAG 4 cut(s) 488, 504, 887, 1095
EcoNI CCTNNNNNAGG 1 cut(s) 183
EcoO109I RGGNCCY 1 cut(s) 799
EcoRII CCWGG 3 cut(s) 442, 532, 1126
EcoRV GATATC 1 cut(s) 608
EcoT14I CCWWGG 1 cut(s) 455
EcoT38I GRGCYC 1 cut(s) 35
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 6 cut(s) 46, 130, 399, 439, 715, 860
FaqI GGGAC 3 cut(s) 556, 812, 1037
FatI CATG 6 cut(s) 42, 126, 395, 435, 711, 856
FblI GTMKAC 2 cut(s) 131, 766
Fnu4HI GCNGC 5 cut(s) 6, 108, 332, 1029, 1064
FokI GGATG 1 cut(s) 864
FriOI GRGCYC 1 cut(s) 35
Fsp4HI GCNGC 5 cut(s) 6, 108, 332, 1029, 1064
FspBI CTAG 2 cut(s) 86, 1173
GluI GCNGC 5 cut(s) 6, 108, 332, 1029, 1064
GsaI CCCAGC 1 cut(s) 80
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 696
HapII CCGG 3 cut(s) 540, 881, 1012
Hin1II CATG 6 cut(s) 46, 130, 399, 439, 715, 860
HincII GTYRAC 2 cut(s) 721, 767
HindII GTYRAC 2 cut(s) 721, 767
HinfI GANTC 7 cut(s) 112, 400, 529, 670, 938, 1097, 1133
HpaII CCGG 3 cut(s) 540, 881, 1012
HphI GGTGA 3 cut(s) 613, 800, 926
Hpy166II GTNNAC 3 cut(s) 132, 721, 767
Hpy188I TCNGA 7 cut(s) 415, 492, 559, 784, 943, 1102, 1183
Hpy188III TCNNGA 4 cut(s) 524, 959, 1073, 1139
Hpy8I GTNNAC 3 cut(s) 132, 721, 767
Hpy99I CGWCG 3 cut(s) 765, 768, 771
HpyAV CCTTC 5 cut(s) 268, 506, 880, 928, 1018
HpyCH4III ACNGT 6 cut(s) 216, 555, 571, 619, 654, 734
HpyCH4V TGCA 6 cut(s) 107, 208, 860, 1049, 1085, 1148
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 317, 862, 1091
HpyF3I CTNAG 4 cut(s) 117, 489, 739, 783
Hsp92II CATG 6 cut(s) 46, 130, 399, 439, 715, 860
KflI GGGWCCC 1 cut(s) 799
KpnI GGTACC 1 cut(s) 991
Kzo9I GATC 1 cut(s) 174
LmnI GCTCC 3 cut(s) 150, 457, 536
Lsp1109I GCAGC 5 cut(s) 17, 119, 318, 1015, 1075
LweI GCATC 4 cut(s) 17, 217, 1036, 1072
MabI ACCWGGT 1 cut(s) 442
MaeI CTAG 2 cut(s) 86, 1173
MaeIII GTNAC 4 cut(s) 226, 280, 613, 619
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 6 cut(s) 204, 279, 402, 572, 783, 1127
MhlI GDGCHC 1 cut(s) 35
MluCI AATT 7 cut(s) 66, 346, 519, 593, 703, 917, 929
MlyI GAGTC 2 cut(s) 947, 1127
MseI TTAA 2 cut(s) 65, 641
MspA1I CMGCKG 2 cut(s) 743, 1066
MspI CCGG 3 cut(s) 540, 881, 1012
MspR9I CCNGG 6 cut(s) 444, 534, 541, 882, 1013, 1128
MvaI CCWGG 3 cut(s) 444, 534, 1128
MwoI GCNNNNNNNGC 4 cut(s) 86, 317, 862, 1091
NciI CCSGG 3 cut(s) 541, 882, 1013
NdeII GATC 1 cut(s) 174
NlaIII CATG 6 cut(s) 46, 130, 399, 439, 715, 860
NlaIV GGNNCC 6 cut(s) 163, 538, 687, 800, 801, 989
NmuCI GTSAC 1 cut(s) 619
NspI RCATGY 1 cut(s) 860
NspV TTCGAA 1 cut(s) 597
PaeI GCATGC 1 cut(s) 860
PcsI WCGNNNNNNNCGW 1 cut(s) 766
PfeI GAWTC 5 cut(s) 112, 400, 529, 670, 1097
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 5 cut(s) 7, 109, 333, 1030, 1065
PleI GAGTC 2 cut(s) 946, 1127
PpsI GAGTC 2 cut(s) 946, 1127
PpuMI RGGWCCY 1 cut(s) 799
PsiI TTATAA 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 3 cut(s) 442, 532, 1126
PspFI CCCAGC 1 cut(s) 76
PspGI CCWGG 3 cut(s) 442, 532, 1126
PspN4I GGNNCC 6 cut(s) 163, 538, 687, 800, 801, 989
PspPI GGNCC 3 cut(s) 446, 695, 799
PspPPI RGGWCCY 1 cut(s) 799
PsrI GAACNNNNNNTAC 2 cut(s) 669, 701
PstI CTGCAG 1 cut(s) 1150
PvuII CAGCTG 1 cut(s) 743
RsaI GTAC 6 cut(s) 651, 656, 678, 736, 747, 989
RsaNI GTAC 6 cut(s) 650, 655, 677, 735, 746, 988
SalI GTCGAC 1 cut(s) 765
SaqAI TTAA 2 cut(s) 65, 641
SatI GCNGC 5 cut(s) 6, 108, 332, 1029, 1064
Sau3AI GATC 1 cut(s) 174
Sau96I GGNCC 3 cut(s) 446, 695, 799
ScaI AGTACT 1 cut(s) 651
SchI GAGTC 2 cut(s) 947, 1127
ScrFI CCNGG 6 cut(s) 444, 534, 541, 882, 1013, 1128
SduI GDGCHC 1 cut(s) 35
SexAI ACCWGGT 1 cut(s) 442
SfaNI GCATC 4 cut(s) 17, 217, 1036, 1072
SfcI CTRYAG 2 cut(s) 1058, 1146
SfuI TTCGAA 1 cut(s) 597
SgrDI CGTCGACG 1 cut(s) 765
SinI GGWCC 2 cut(s) 446, 799
SmlI CTYRAG 2 cut(s) 390, 1032
SmoI CTYRAG 2 cut(s) 390, 1032
SphI GCATGC 1 cut(s) 860
Sse9I AATT 7 cut(s) 66, 346, 519, 593, 703, 917, 929
SsiI CCGC 4 cut(s) 285, 334, 603, 1066
SspI AATATT 1 cut(s) 341
SspMI CTAG 2 cut(s) 86, 1173
StyD4I CCNGG 6 cut(s) 442, 532, 539, 880, 1011, 1126
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 6 cut(s) 216, 555, 571, 619, 654, 734
TaqI TCGA 3 cut(s) 211, 597, 766
TasI AATT 7 cut(s) 66, 346, 519, 593, 703, 917, 929
TatI WGTACW 2 cut(s) 649, 676
TfiI GAWTC 5 cut(s) 112, 400, 529, 670, 1097
Tru1I TTAA 2 cut(s) 65, 641
Tru9I TTAA 2 cut(s) 65, 641
TscAI CASTG 2 cut(s) 574, 1048
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 5 cut(s) 5, 107, 331, 1028, 1063
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 5 cut(s) 59, 115, 381, 866, 1167
TspRI CASTG 2 cut(s) 574, 1048
VpaK11BI GGWCC 2 cut(s) 446, 799
XagI CCTNNNNNAGG 1 cut(s) 183
XapI RAATTY 1 cut(s) 593
XceI RCATGY 1 cut(s) 860
XmiI GTMKAC 2 cut(s) 131, 766
XspI CTAG 2 cut(s) 86, 1173
ZrmI AGTACT 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.