MD17G1076300.v1.1

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003334
Physical Location & Seq
Forward (+)
6332261 .. 6334201
1941 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1076300.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGAAGGAAGAAGAAGTGAATAGATGCCAGATACAGGAATGGTACCCGAAGTTTAAATCCGTGTCTATCAGAACCCGAATTCATGAGCTTCCTGAATCCTTTGTCCAGTACCTTCTCGATGATTCGGGCCCCTTCCTTCTCCCTGTTTCAATCTCAAATGATGATGCATTTCCTAATAGAATTCATAATCCGGAGGAGGAAGACGACTATCAAGTATCAGAAGGGTCTGGTGATGAGTCGGAGCAGCCTTCTCTGCCGCCCTCTTTCCCTGAGCTTGAGTTGGAGATCAAGGAATCGATTGAGTCCCTTGGAGGGTCTGTGTTCCCTAAGCTGAACTGGAGTGCACCAAAAGACTCGGCTTGGATCAGCACAACTGGGAGTCTCAGATGCTCCTCGTTCAGTGAGATTGCGCTCTTGCTACGATCGTCTGACTCATTGGTCCACGATCTGTGTCACGCATATGATTCCTGCAGTGACAAAACCTCATCAAGGCCTAGGAGTTTCTTCCTTGCACTTCGGAAATGGTACCAATCTCTCAAGCCAGAGATGGAGTTCCGTTGCTTTGTACGAAATCAGAACTTAGTTGGAATTTCCCAACGTGAGGTCACTACTTGTTATCCTGCTCTGATTGAAAAGAAAGACAGCCTTCAAGCATTGATCGAAGACTTTTTCTTGGATAATTTGATGTTGAGATTTGAATCAGAAAACTACACCTTTGATGTCTATGTGACAAAGGATGATCGCGTTAAGGTTGTGGATTTCAACCCATGGGGCGCATTTACGCTACCTCTGCTGTATACATGGGAGGAATTGGATCAGACTCATAGTGAACAAGGAGATGGTGTCGACTTCAGAGTTGTAGAGAGCCAGTTATCTGTTAGGCCAGGTTTGAAGACAGCAGTGCCGTTTGATTATTTGGACACTGGCACCGGGAGTGGCTGGGATCAAGTTATCAGAAATGCTAATGAAGAGTTGCAGCAACAAACTAGGAATGCTCAAGCAGGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

337

Amino Acids

38.43

Weight (kDa)

4.57

Isoelectric Point (pI)

55.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 14 - 307 2.3e-96 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 992
AasI GACNNNNNNGTC 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 992
Acc65I GGTACC 2 cut(s) 42, 525
AccB1I GGYRCC 3 cut(s) 42, 525, 926
AccI GTMKAC 2 cut(s) 797, 846
AccII CGCG 1 cut(s) 744
AccIII TCCGGA 1 cut(s) 190
AciI CCGC 1 cut(s) 257
AclWI GGATC 3 cut(s) 371, 822, 951
AcsI RAATTY 3 cut(s) 78, 180, 588
AcuI CTGAAG 1 cut(s) 835
AfaI GTAC 4 cut(s) 44, 110, 527, 567
AfiI CCNNNNNNNGG 4 cut(s) 34, 312, 489, 601
AgsI TTSAA 6 cut(s) 150, 632, 650, 698, 763, 892
AjnI CCWGG 1 cut(s) 883
AluBI AGCT 3 cut(s) 88, 274, 331
AluI AGCT 3 cut(s) 88, 274, 331
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 1 cut(s) 386
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 3 cut(s) 371, 822, 951
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 3 cut(s) 127, 491, 881
ApaI GGGCCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 244, 976
ApoI RAATTY 3 cut(s) 78, 180, 588
Asp718I GGTACC 2 cut(s) 42, 525
AspA2I CCTAGG 1 cut(s) 494
AspLEI GCGC 2 cut(s) 412, 776
AspS9I GGNCC 3 cut(s) 127, 128, 439
AsuC2I CCSGG 1 cut(s) 931
AsuHPI GGTGA 1 cut(s) 242
AvaII GGWCC 1 cut(s) 439
AvrII CCTAGG 1 cut(s) 494
BaeGI GKGCMC 2 cut(s) 131, 346
BanI GGYRCC 3 cut(s) 42, 525, 926
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 3 cut(s) 207, 669, 899
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 256, 988
BccI CCATC 2 cut(s) 541, 833
BceAI ACGGC 1 cut(s) 889
BciT130I CCWGG 1 cut(s) 885
BcnI CCSGG 1 cut(s) 931
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 2 cut(s) 495, 987
BfmI CTRYAG 1 cut(s) 469
BfuAI ACCTGC 1 cut(s) 992
BisI GCNGC 3 cut(s) 245, 257, 977
BlnI CCTAGG 1 cut(s) 494
BlsI GCNGC 3 cut(s) 246, 258, 978
Bme1390I CCNGG 2 cut(s) 885, 931
Bme18I GGWCC 1 cut(s) 439
BmgT120I GGNCC 3 cut(s) 127, 128, 439
BmiI GGNNCC 5 cut(s) 44, 129, 130, 527, 928
BmrFI CCNGG 2 cut(s) 885, 931
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 3 cut(s) 14, 154, 377
BmuI ACTGGG 1 cut(s) 384
BpiI GAAGAC 3 cut(s) 207, 669, 899
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 2 cut(s) 270, 327
BpuEI CTTGAG 3 cut(s) 296, 521, 981
BpuMI CCSGG 1 cut(s) 931
Bsa29I ATCGAT 1 cut(s) 296
BsaBI GATNNNNATC 1 cut(s) 697
BsaJI CCNNGG 3 cut(s) 307, 494, 767
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 828, 858
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 312, 489, 601
Bse1I ACTGG 5 cut(s) 106, 341, 379, 868, 928
Bse8I GATNNNNATC 1 cut(s) 697
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 307, 494, 767
BseGI GGATG 1 cut(s) 742
BseJI GATNNNNATC 1 cut(s) 697
BseLI CCNNNNNNNGG 4 cut(s) 34, 312, 489, 601
BseMII CTCAG 2 cut(s) 261, 397
BseNI ACTGG 5 cut(s) 106, 341, 379, 868, 928
BseRI GAGGAG 2 cut(s) 209, 382
BseSI GKGCMC 2 cut(s) 131, 346
BseXI GCAGC 2 cut(s) 256, 988
BseYI CCCAGC 1 cut(s) 939
Bsh1236I CGCG 1 cut(s) 744
Bsh1285I CGRYCG 1 cut(s) 425
BshFI GGCC 3 cut(s) 129, 493, 883
BshNI GGYRCC 3 cut(s) 42, 525, 926
BshVI ATCGAT 1 cut(s) 296
BsiEI CGRYCG 1 cut(s) 425
BsiHKAI GWGCWC 1 cut(s) 346
BsiSI CCGG 2 cut(s) 191, 930
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 4 cut(s) 34, 312, 489, 601
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 289
BsmI GAATGC 1 cut(s) 997
BsnI GGCC 3 cut(s) 129, 493, 883
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 131, 346
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
Bsp19I CCATGG 1 cut(s) 767
BspACI CCGC 1 cut(s) 257
BspANI GGCC 3 cut(s) 129, 493, 883
BspCNI CTCAG 2 cut(s) 262, 396
BspDI ATCGAT 1 cut(s) 296
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 1 cut(s) 744
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 5 cut(s) 44, 129, 130, 527, 928
BspMAI CTGCAG 1 cut(s) 473
BspMI ACCTGC 1 cut(s) 992
BspPI GGATC 3 cut(s) 371, 822, 951
BspT107I GGYRCC 3 cut(s) 42, 525, 926
BsrI ACTGG 5 cut(s) 106, 341, 379, 868, 928
BssECI CCNNGG 3 cut(s) 307, 494, 767
BssMI GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
BssNAI GTATAC 1 cut(s) 798
BssT1I CCWWGG 3 cut(s) 307, 494, 767
Bst1107I GTATAC 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 885
Bst6I CTCTTC 1 cut(s) 963
BstDEI CTNAG 4 cut(s) 270, 327, 383, 580
BstDSI CCRYGG 1 cut(s) 767
BstENI CCTNNNNNAGG 1 cut(s) 487
BstF5I GGATG 1 cut(s) 742
BstFNI CGCG 1 cut(s) 744
BstHHI GCGC 2 cut(s) 412, 776
BstKTI GATC 8 cut(s) 288, 366, 425, 448, 660, 742, 817, 946
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
BstMCI CGRYCG 1 cut(s) 425
BstMWI GCNNNNNNNGC 2 cut(s) 253, 790
BstNI CCWGG 1 cut(s) 885
BstSCI CCNGG 2 cut(s) 883, 929
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 2 cut(s) 131, 346
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 2 cut(s) 256, 988
BstV2I GAAGAC 3 cut(s) 207, 669, 899
BstZ17I GTATAC 1 cut(s) 798
Bsu15I ATCGAT 1 cut(s) 296
BsuRI GGCC 3 cut(s) 129, 493, 883
BsuTUI ATCGAT 1 cut(s) 296
BtgI CCRYGG 1 cut(s) 767
BtsCI GGATG 1 cut(s) 742
BtsI GCAGTG 2 cut(s) 478, 906
BtsIMutI CAGTG 4 cut(s) 406, 478, 906, 921
BveI ACCTGC 1 cut(s) 992
CciI TCATGA 1 cut(s) 82
CfoI GCGC 2 cut(s) 412, 776
Cfr13I GGNCC 3 cut(s) 127, 128, 439
ClaI ATCGAT 1 cut(s) 296
Csp6I GTAC 4 cut(s) 43, 109, 526, 566
CviAII CATG 3 cut(s) 83, 768, 801
CviQI GTAC 4 cut(s) 43, 109, 526, 566
DdeI CTNAG 4 cut(s) 270, 327, 383, 580
DpnI GATC 8 cut(s) 287, 365, 424, 447, 659, 741, 816, 945
DpnII GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
DraI TTTAAA 1 cut(s) 55
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Eco130I CCWWGG 3 cut(s) 307, 494, 767
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 131
Eco47I GGWCC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 835
EcoNI CCTNNNNNAGG 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 128
EcoRI GAATTC 2 cut(s) 78, 180
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 3 cut(s) 307, 494, 767
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 3 cut(s) 307, 494, 767
FaeI CATG 3 cut(s) 86, 771, 804
FaiI YATR 9 cut(s) 84, 186, 460, 462, 726, 769, 798, 802, 825
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 1 cut(s) 289
FatI CATG 3 cut(s) 82, 767, 800
FauNDI CATATG 1 cut(s) 460
FblI GTMKAC 2 cut(s) 797, 846
Fnu4HI GCNGC 3 cut(s) 245, 257, 977
FokI GGATG 1 cut(s) 749
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 245, 257, 977
FspBI CTAG 2 cut(s) 495, 987
GlaI GCGC 2 cut(s) 411, 775
GluI GCNGC 3 cut(s) 245, 257, 977
GsaI CCCAGC 1 cut(s) 943
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 3 cut(s) 129, 493, 883
HapII CCGG 2 cut(s) 191, 930
HhaI GCGC 2 cut(s) 412, 776
Hin1II CATG 3 cut(s) 86, 771, 804
Hin6I GCGC 2 cut(s) 410, 774
HinP1I GCGC 2 cut(s) 410, 774
HincII GTYRAC 1 cut(s) 847
HindII GTYRAC 1 cut(s) 847
HpaII CCGG 2 cut(s) 191, 930
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 5 cut(s) 344, 442, 798, 830, 847
Hpy188III TCNNGA 4 cut(s) 83, 92, 116, 191
Hpy8I GTNNAC 5 cut(s) 344, 442, 798, 830, 847
HpyAV CCTTC 6 cut(s) 122, 142, 146, 215, 258, 656
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 5 cut(s) 167, 344, 471, 512, 976
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 790
HpyF3I CTNAG 4 cut(s) 270, 327, 383, 580
HpySE526I ACGT 1 cut(s) 598
Hsp92II CATG 3 cut(s) 86, 771, 804
HspAI GCGC 2 cut(s) 410, 774
Kpn2I TCCGGA 1 cut(s) 190
KpnI GGTACC 2 cut(s) 46, 529
Kzo9I GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
LmnI GCTCC 2 cut(s) 241, 395
Lsp1109I GCAGC 2 cut(s) 256, 988
LweI GCATC 3 cut(s) 14, 154, 377
MaeI CTAG 2 cut(s) 495, 987
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 4 cut(s) 452, 473, 604, 727
MalI GATC 8 cut(s) 287, 365, 424, 447, 659, 741, 816, 945
MboI GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
MboII GAAGA 7 cut(s) 20, 23, 212, 496, 674, 904, 980
MhlI GDGCHC 2 cut(s) 131, 346
MluCI AATT 5 cut(s) 78, 180, 588, 679, 809
MlyI GAGTC 6 cut(s) 245, 311, 347, 388, 425, 814
MmeI TCCRAC 3 cut(s) 219, 261, 565
MnlI CCTC 9 cut(s) 187, 190, 271, 305, 403, 493, 595, 798, 799
Mph1103I ATGCAT 1 cut(s) 169
MroI TCCGGA 1 cut(s) 190
MseI TTAA 3 cut(s) 54, 747, 1009
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 2 cut(s) 191, 930
MspR9I CCNGG 2 cut(s) 885, 931
Mva1269I GAATGC 1 cut(s) 997
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 2 cut(s) 253, 790
NciI CCSGG 1 cut(s) 931
NcoI CCATGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 460
NdeII GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
NlaIII CATG 3 cut(s) 86, 771, 804
NlaIV GGNNCC 5 cut(s) 44, 129, 130, 527, 928
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 4 cut(s) 452, 473, 604, 727
NsiI ATGCAT 1 cut(s) 169
PagI TCATGA 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 992
PceI AGGCCT 1 cut(s) 493
PctI GAATGC 1 cut(s) 997
PfeI GAWTC 5 cut(s) 95, 122, 293, 464, 698
PkrI GCNGC 3 cut(s) 246, 258, 978
Ple19I CGATCG 1 cut(s) 425
PleI GAGTC 6 cut(s) 244, 310, 347, 387, 425, 814
PpsI GAGTC 6 cut(s) 244, 310, 347, 387, 425, 814
Psp6I CCWGG 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 939
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 5 cut(s) 44, 129, 130, 527, 928
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 3 cut(s) 127, 128, 439
PstI CTGCAG 1 cut(s) 473
PvuI CGATCG 1 cut(s) 425
RsaI GTAC 4 cut(s) 44, 110, 527, 567
RsaNI GTAC 4 cut(s) 43, 109, 526, 566
RseI CAYNNNNRTG 1 cut(s) 459
SalI GTCGAC 1 cut(s) 845
SaqAI TTAA 3 cut(s) 54, 747, 1009
SatI GCNGC 3 cut(s) 245, 257, 977
Sau3AI GATC 8 cut(s) 285, 363, 422, 445, 657, 739, 814, 943
Sau96I GGNCC 3 cut(s) 127, 128, 439
SchI GAGTC 6 cut(s) 245, 311, 347, 388, 425, 814
ScrFI CCNGG 2 cut(s) 885, 931
SduI GDGCHC 2 cut(s) 131, 346
SfaNI GCATC 3 cut(s) 14, 154, 377
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 3 cut(s) 275, 536, 996
SmoI CTYRAG 3 cut(s) 275, 536, 996
Sse9I AATT 5 cut(s) 78, 180, 588, 679, 809
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 1 cut(s) 257
SspMI CTAG 2 cut(s) 495, 987
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 2 cut(s) 883, 929
StyI CCWWGG 3 cut(s) 307, 494, 767
TaiI ACGT 1 cut(s) 601
TaqI TCGA 4 cut(s) 117, 296, 660, 846
TasI AATT 5 cut(s) 78, 180, 588, 679, 809
TauI GCSGC 1 cut(s) 259
TfiI GAWTC 5 cut(s) 95, 122, 293, 464, 698
Tru1I TTAA 3 cut(s) 54, 747, 1009
Tru9I TTAA 3 cut(s) 54, 747, 1009
TscAI CASTG 4 cut(s) 406, 478, 906, 928
TseFI GTSAC 4 cut(s) 452, 473, 604, 727
TseI GCWGC 2 cut(s) 244, 976
Tsp45I GTSAC 4 cut(s) 452, 473, 604, 727
TspDTI ATGAA 4 cut(s) 17, 71, 173, 981
TspGWI ACGGA 2 cut(s) 49, 545
TspRI CASTG 4 cut(s) 406, 478, 906, 928
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 439
XagI CCTNNNNNAGG 1 cut(s) 487
XapI RAATTY 3 cut(s) 78, 180, 588
XmaJI CCTAGG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 797, 846
XspI CTAG 2 cut(s) 495, 987
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.