Rh5AG343000

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003334
Physical Location & Seq
Forward (+)
56099374 .. 56100369
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG343000.1

Sequence Viewer

Length: 996 bp
ATGAAGGAAGAAGTGAATCGATGCCAGATACAGGAATGGTACCCTAAGTTTAAATCCGTGTCTATCAAAACACGGATTCATGAACTTCCAGAATCCTTTGTCCAATATCTTCGTGATGATTCAGGGCCCTTCCTTCTTCCAGTTTCCATCTCAAATGAAGATGCCTTACCCAACAGAATTCATAATCCAGAGGAGGAAGATGACTATCAAGTAATAGAAGGATCTGATGATGAGTCAGAGCAGCCTTCTTTGCCCCCTTCTTTTCCTGAACTTGAGTTGGAGACTAAGGAATCCATTGCATCGCTTGGAGGTGCTGTGTTCCCGAAGCTAAACTGGAGTGCACCAAAAGATTCTGCTTGGATTAGCACAACTGGGACTCTCAGATGCTCTTCCTTCAGTGAGATTGCACTGTTGCTACGATCATCTGACTCACTGGTGCATGATTTGTGTCATGCTTATGATTCCTGCAATGACAAGACCTCCTCACGGCCCAAGAGCTTCTTCCTTGCTCTCCGTAAATGGTACCCATCCCTTCAGCCAGAGATGGAATTCCGCTGCTTTGTGAGGAATCAGAATTTAGTTGGAATTTCCCAGCGCGAGGCCACAACCTTCTACCCTTCTCTGCTTAAAAGCAAAGAGCATCTGGGAGAGTTGATCGAAGAGTTCTTTATGGAAAATTTAAGGTCAAGATTCGAGTCAGAAAATTATACCTTTGATGTATACGTGACGAAGGACAAGCGAGTTAAGGTTATGGATTTCAACCCTTGGGGTGCATTTACACTGCCTCTGATGTTTACATGGGAGGAACTGGAACAGAGTGATAATGAACAAGGGGATGGTGTGGACTTCAGAATTGTGGAGAGCCAGTGTGGTGTTAGGCCAGGTTTGAAGACAGCAGTGCCCTACGATTATGTGGACACTAGCTCTGGGAGTGGCTGGGACCAGTTTTTCAGGAACGCCGATAAAGAGTTACAGCGGCGAAGTAGGTCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

331

Amino Acids

38.2

Weight (kDa)

4.81

Isoelectric Point (pI)

61.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 13 - 308 9.2e-99 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 39, 522
AccB1I GGYRCC 2 cut(s) 39, 522
AccI GTMKAC 1 cut(s) 720
AccII CGCG 1 cut(s) 597
AciI CCGC 2 cut(s) 553, 976
AclWI GGATC 1 cut(s) 229
AcsI RAATTY 5 cut(s) 177, 548, 574, 585, 676
AcuI CTGAAG 3 cut(s) 379, 518, 832
AfaI GTAC 2 cut(s) 41, 524
AfiI CCNNNNNNNGG 3 cut(s) 31, 486, 598
AgsI TTSAA 2 cut(s) 760, 889
AjnI CCWGG 1 cut(s) 880
AjuI GAANNNNNNNTTGG 2 cut(s) 485, 517
AluBI AGCT 3 cut(s) 328, 498, 924
AluI AGCT 3 cut(s) 328, 498, 924
Alw21I GWGCWC 1 cut(s) 343
Alw26I GTCTC 1 cut(s) 275
Alw44I GTGCAC 1 cut(s) 339
AlwI GGATC 1 cut(s) 229
AoxI GGCC 4 cut(s) 125, 488, 600, 878
ApaI GGGCCC 1 cut(s) 129
ApaLI GTGCAC 1 cut(s) 339
ApeKI GCWGC 2 cut(s) 241, 555
ApoI RAATTY 5 cut(s) 177, 548, 574, 585, 676
Asp718I GGTACC 2 cut(s) 39, 522
AspLEI GCGC 1 cut(s) 597
AspS9I GGNCC 4 cut(s) 125, 126, 489, 940
AvaII GGWCC 1 cut(s) 940
BaeGI GKGCMC 3 cut(s) 129, 343, 903
BanI GGYRCC 2 cut(s) 39, 522
BanII GRGCYC 1 cut(s) 129
BarI GAAGNNNNNNTAC 2 cut(s) 150, 182
BbsI GAAGAC 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 343
BbvI GCAGC 2 cut(s) 253, 542
BccI CCATC 4 cut(s) 155, 535, 538, 830
BceAI ACGGC 1 cut(s) 503
BciT130I CCWGG 1 cut(s) 882
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 1 cut(s) 921
BisI GCNGC 3 cut(s) 242, 556, 977
BlsI GCNGC 3 cut(s) 243, 557, 978
Bme1390I CCNGG 1 cut(s) 882
Bme18I GGWCC 1 cut(s) 940
BmgT120I GGNCC 4 cut(s) 125, 126, 489, 940
BmiI GGNNCC 4 cut(s) 41, 127, 524, 941
BmrFI CCNGG 1 cut(s) 882
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 5 cut(s) 11, 151, 308, 374, 649
BmuI ACTGGG 1 cut(s) 381
BpiI GAAGAC 1 cut(s) 896
BpmI CTGGAG 1 cut(s) 355
BpuEI CTTGAG 1 cut(s) 293
Bsa29I ATCGAT 1 cut(s) 19
BsaAI YACGTR 1 cut(s) 724
BsaBI GATNNNNATC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 764
BsaXI ACNNNNNCTCC 6 cut(s) 300, 330, 464, 494, 851, 881
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 486, 598
Bse1I ACTGG 7 cut(s) 140, 338, 376, 438, 813, 865, 943
Bse3DI GCAATG 2 cut(s) 294, 475
Bse8I GATNNNNATC 1 cut(s) 204
BseBI CCWGG 1 cut(s) 882
BseCI ATCGAT 1 cut(s) 19
BseDI CCNNGG 1 cut(s) 764
BseGI GGATG 2 cut(s) 527, 841
BseJI GATNNNNATC 1 cut(s) 204
BseLI CCNNNNNNNGG 3 cut(s) 31, 486, 598
BseMI GCAATG 2 cut(s) 294, 475
BseMII CTCAG 1 cut(s) 394
BseNI ACTGG 7 cut(s) 140, 338, 376, 438, 813, 865, 943
BseRI GAGGAG 2 cut(s) 206, 472
BseSI GKGCMC 3 cut(s) 129, 343, 903
BseXI GCAGC 2 cut(s) 253, 542
BseYI CCCAGC 2 cut(s) 591, 936
Bsh1236I CGCG 1 cut(s) 597
BshFI GGCC 4 cut(s) 127, 490, 602, 880
BshNI GGYRCC 2 cut(s) 39, 522
BshVI ATCGAT 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 343
BslFI GGGAC 2 cut(s) 388, 953
BslI CCNNNNNNNGG 3 cut(s) 31, 486, 598
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 2 cut(s) 388, 953
BsnI GGCC 4 cut(s) 127, 490, 602, 880
Bsp120I GGGCCC 1 cut(s) 125
Bsp1286I GDGCHC 3 cut(s) 129, 343, 903
Bsp143I GATC 3 cut(s) 221, 419, 654
BspACI CCGC 2 cut(s) 553, 976
BspANI GGCC 4 cut(s) 127, 490, 602, 880
BspCNI CTCAG 1 cut(s) 393
BspDI ATCGAT 1 cut(s) 19
BspFNI CGCG 1 cut(s) 597
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 4 cut(s) 41, 127, 524, 941
BspPI GGATC 1 cut(s) 229
BspQI GCTCTTC 1 cut(s) 394
BspT107I GGYRCC 2 cut(s) 39, 522
BsrDI GCAATG 2 cut(s) 294, 475
BsrI ACTGG 7 cut(s) 140, 338, 376, 438, 813, 865, 943
BssECI CCNNGG 1 cut(s) 764
BssMI GATC 3 cut(s) 221, 419, 654
BssNAI GTATAC 1 cut(s) 721
BssT1I CCWWGG 1 cut(s) 764
Bst1107I GTATAC 1 cut(s) 721
Bst2UI CCWGG 1 cut(s) 882
Bst4CI ACNGT 1 cut(s) 411
Bst6I CTCTTC 2 cut(s) 394, 654
BstBAI YACGTR 1 cut(s) 724
BstDEI CTNAG 3 cut(s) 45, 285, 380
BstF5I GGATG 2 cut(s) 527, 841
BstFNI CGCG 1 cut(s) 597
BstHHI GCGC 1 cut(s) 597
BstKTI GATC 3 cut(s) 224, 422, 657
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 3 cut(s) 221, 419, 654
BstMWI GCNNNNNNNGC 1 cut(s) 250
BstNI CCWGG 1 cut(s) 882
BstSCI CCNGG 1 cut(s) 880
BstSLI GKGCMC 3 cut(s) 129, 343, 903
BstUI CGCG 1 cut(s) 597
BstV1I GCAGC 2 cut(s) 253, 542
BstV2I GAAGAC 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
BstZ17I GTATAC 1 cut(s) 721
Bsu15I ATCGAT 1 cut(s) 19
BsuRI GGCC 4 cut(s) 127, 490, 602, 880
BsuTUI ATCGAT 1 cut(s) 19
BtgZI GCGATG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 527, 841
BtsI GCAGTG 2 cut(s) 779, 903
BtsIMutI CAGTG 6 cut(s) 403, 407, 431, 779, 872, 903
CciI TCATGA 1 cut(s) 79
CfoI GCGC 1 cut(s) 597
Cfr13I GGNCC 4 cut(s) 125, 126, 489, 940
ClaI ATCGAT 1 cut(s) 19
Csp6I GTAC 2 cut(s) 40, 523
CviAII CATG 4 cut(s) 80, 440, 452, 798
CviQI GTAC 2 cut(s) 40, 523
DdeI CTNAG 3 cut(s) 45, 285, 380
DpnI GATC 3 cut(s) 223, 421, 656
DpnII GATC 3 cut(s) 221, 419, 654
DraI TTTAAA 1 cut(s) 52
Eam1104I CTCTTC 2 cut(s) 394, 654
EarI CTCTTC 2 cut(s) 394, 654
Eco130I CCWWGG 1 cut(s) 764
Eco24I GRGCYC 1 cut(s) 129
Eco47I GGWCC 1 cut(s) 940
Eco57I CTGAAG 3 cut(s) 379, 518, 832
EcoO109I RGGNCCY 2 cut(s) 125, 126
EcoRI GAATTC 2 cut(s) 177, 548
EcoRII CCWGG 1 cut(s) 880
EcoT14I CCWWGG 1 cut(s) 764
EcoT38I GRGCYC 1 cut(s) 129
ErhI CCWWGG 1 cut(s) 764
FaeI CATG 4 cut(s) 83, 443, 455, 801
FalI AAGNNNNNCTT 2 cut(s) 485, 517
FaqI GGGAC 2 cut(s) 388, 953
FatI CATG 4 cut(s) 79, 439, 451, 797
FblI GTMKAC 1 cut(s) 720
Fnu4HI GCNGC 3 cut(s) 242, 556, 977
FokI GGATG 2 cut(s) 514, 848
FriOI GRGCYC 1 cut(s) 129
Fsp4HI GCNGC 3 cut(s) 242, 556, 977
FspBI CTAG 1 cut(s) 921
GlaI GCGC 1 cut(s) 596
GluI GCNGC 3 cut(s) 242, 556, 977
GsaI CCCAGC 2 cut(s) 595, 940
GsuI CTGGAG 1 cut(s) 355
HaeIII GGCC 4 cut(s) 127, 490, 602, 880
HhaI GCGC 1 cut(s) 597
Hin1II CATG 4 cut(s) 83, 443, 455, 801
Hin6I GCGC 1 cut(s) 595
HinP1I GCGC 1 cut(s) 595
Hpy166II GTNNAC 5 cut(s) 341, 721, 795, 844, 916
Hpy188I TCNGA 8 cut(s) 226, 238, 383, 427, 573, 700, 789, 851
Hpy188III TCNNGA 8 cut(s) 80, 89, 113, 188, 266, 322, 687, 952
Hpy8I GTNNAC 5 cut(s) 341, 721, 795, 844, 916
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4IV ACGT 1 cut(s) 723
HpyCH4V TGCA 6 cut(s) 299, 341, 407, 439, 468, 773
HpyF10VI GCNNNNNNNGC 1 cut(s) 250
HpyF3I CTNAG 3 cut(s) 45, 285, 380
HpySE526I ACGT 1 cut(s) 723
Hsp92II CATG 4 cut(s) 83, 443, 455, 801
HspAI GCGC 1 cut(s) 595
KpnI GGTACC 2 cut(s) 43, 526
Kzo9I GATC 3 cut(s) 221, 419, 654
LguI GCTCTTC 1 cut(s) 394
Lsp1109I GCAGC 2 cut(s) 253, 542
LweI GCATC 5 cut(s) 11, 151, 308, 374, 649
MaeI CTAG 1 cut(s) 921
MaeII ACGT 1 cut(s) 723
MaeIII GTNAC 2 cut(s) 724, 969
MalI GATC 3 cut(s) 223, 421, 656
MboI GATC 3 cut(s) 221, 419, 654
MboII GAAGA 9 cut(s) 20, 101, 128, 170, 209, 381, 493, 671, 901
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 3 cut(s) 129, 343, 903
MluCI AATT 7 cut(s) 177, 548, 574, 585, 676, 703, 852
MlyI GAGTC 4 cut(s) 242, 370, 422, 704
MmeI TCCRAC 2 cut(s) 258, 562
MnlI CCTC 9 cut(s) 184, 187, 302, 490, 493, 558, 592, 795, 796
MseI TTAA 5 cut(s) 51, 627, 680, 744, 994
MslI CAYNNNNRTG 1 cut(s) 456
MspA1I CMGCKG 2 cut(s) 555, 976
MspR9I CCNGG 1 cut(s) 882
MvaI CCWGG 1 cut(s) 882
MvnI CGCG 1 cut(s) 597
MwoI GCNNNNNNNGC 1 cut(s) 250
NdeII GATC 3 cut(s) 221, 419, 654
NlaIII CATG 4 cut(s) 83, 443, 455, 801
NlaIV GGNNCC 4 cut(s) 41, 127, 524, 941
NmuCI GTSAC 1 cut(s) 724
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 394
PfeI GAWTC 9 cut(s) 16, 76, 92, 119, 290, 350, 461, 568, 690
PkrI GCNGC 3 cut(s) 243, 557, 978
PleI GAGTC 4 cut(s) 241, 370, 422, 703
PpsI GAGTC 4 cut(s) 241, 370, 422, 703
Ppu21I YACGTR 1 cut(s) 724
Psp6I CCWGG 1 cut(s) 880
PspFI CCCAGC 2 cut(s) 591, 936
PspGI CCWGG 1 cut(s) 880
PspN4I GGNNCC 4 cut(s) 41, 127, 524, 941
PspOMI GGGCCC 1 cut(s) 125
PspPI GGNCC 4 cut(s) 125, 126, 489, 940
PsuI RGATCY 1 cut(s) 221
RsaI GTAC 2 cut(s) 41, 524
RsaNI GTAC 2 cut(s) 40, 523
RseI CAYNNNNRTG 1 cut(s) 456
SapI GCTCTTC 1 cut(s) 394
SaqAI TTAA 5 cut(s) 51, 627, 680, 744, 994
SatI GCNGC 3 cut(s) 242, 556, 977
Sau3AI GATC 3 cut(s) 221, 419, 654
Sau96I GGNCC 4 cut(s) 125, 126, 489, 940
SchI GAGTC 4 cut(s) 242, 370, 422, 704
ScrFI CCNGG 1 cut(s) 882
SduI GDGCHC 3 cut(s) 129, 343, 903
SfaNI GCATC 5 cut(s) 11, 151, 308, 374, 649
SinI GGWCC 1 cut(s) 940
SmiMI CAYNNNNRTG 1 cut(s) 456
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
Sse9I AATT 7 cut(s) 177, 548, 574, 585, 676, 703, 852
SsiI CCGC 2 cut(s) 553, 976
SspMI CTAG 1 cut(s) 921
StyD4I CCNGG 1 cut(s) 880
StyI CCWWGG 1 cut(s) 764
TaaI ACNGT 1 cut(s) 411
TaiI ACGT 1 cut(s) 726
TaqI TCGA 3 cut(s) 19, 657, 693
TasI AATT 7 cut(s) 177, 548, 574, 585, 676, 703, 852
TauI GCSGC 1 cut(s) 979
TfiI GAWTC 9 cut(s) 16, 76, 92, 119, 290, 350, 461, 568, 690
Tru1I TTAA 5 cut(s) 51, 627, 680, 744, 994
Tru9I TTAA 5 cut(s) 51, 627, 680, 744, 994
TscAI CASTG 6 cut(s) 403, 414, 438, 786, 872, 903
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 2 cut(s) 241, 555
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 6 cut(s) 17, 68, 96, 170, 171, 840
TspGWI ACGGA 3 cut(s) 46, 88, 503
TspRI CASTG 6 cut(s) 403, 414, 438, 786, 872, 903
VneI GTGCAC 1 cut(s) 339
VpaK11BI GGWCC 1 cut(s) 940
XapI RAATTY 5 cut(s) 177, 548, 574, 585, 676
XmiI GTMKAC 1 cut(s) 720
XspI CTAG 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.