RLG00000032197

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003334
Physical Location & Seq
Reverse (-)
11972743 .. 11975194
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032197

Sequence Viewer

Length: 1221 bp
ATGAAGGAAGAAGAAGTGAATCGATGCCAGATACAGGAATGGTACCCTAAGTTTAAATCCGTGTCTATCAAAACACGGATTCATGAACTTCCAGAATCCTTTGTCCAATATCTTCTTGATGATTCAGGGCCCTTCCTTCTTCCAGTTTCCATCTCAAATGAAGATGCCTTACCTAACAGAATTCATAATCCAGAGGAGGAAGATGACTATCAAGTAGTAGAAGGATCTGATGATGAGTCAGAGCAGCCTTCGTCGCCCCCTTCTTTTCCTGAACTTGAGTTGGAGATTAAGGAATCCATTGCATCGCTTGGAGGTGCTGTGTTCCCGAAGCTAAACTGGAGTGCACCAAAAGATTCTGCTTGGATTAGCACAACAGGGACTCTCAGATGCTCTTCCTTCAGTGAGATTGCACTGTTGCTACGATCATCTGACTCACTGGTGCATGATTTGTGTCATGCGTATGATTCCTGCAATGACAAGACCTCCTCACGGCCCAAGAGCTTCTTCCTTGCTCTCCGTAAATGGTACCCATCCCTTCAACCAGAGATGGAATTCCGCTGCTTTGTAAGGAATCAGAATTTAGTTGGAATTTCCCAACGCGAGGCCACAACCTTCTACCCTTCTCTGCTTGAAAGCAAAGAGCATCTTGGAGTGTTGATTGAAGACTTCTTTATGGAAAATTTAAGGTCAAGATTTGAGTCAGAAAATTACACCTTTGATGTGTATGTGACGAAGGACGAGCGAGTTAAGGTTATGGATTTCAACCCTTGGGGAGCATTTACACTGCCTCTGATGTTTACATGGGAGGAACTGGAACAGAATGATAATGAACAAGGAGTTGGTGTGGACTTCAGAATTGTGGAGAACCAGTGTGGTGTTAGGCCAGGTTTGAAGACAGCAGTGCCCTACGATTATCTGGACACTAGCTCCGGGAGTGGCTGGGACCAGTTTTTCAGGAACGCCAATGAAGAGTTACAGCGTCAAAGTAGATCTCCTGAGGCAGATCACGCCGACTCCCCCAAACTGAAGGTCGAGATCCGTTTCAATCTCCTCCGACCCCAAACCAGCAACCAAGTTCCAAATCCCAACCCCTCTAGGTCTGCAAGATCTGCAACCCCACCCCTCAGCGCCCCTGAACTCTCCTTCCTCCTCCCATCTACCATTGTCGCCTACGGCCACAGCCCGTCCGACGACAGCGGGAGTGACAGCAACAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

407

Amino Acids

46.13

Weight (kDa)

4.7

Isoelectric Point (pI)

61.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 14 - 308 3e-98 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 42, 525
AccB1I GGYRCC 2 cut(s) 42, 525
AccII CGCG 1 cut(s) 600
AciI CCGC 2 cut(s) 556, 1197
AclWI GGATC 2 cut(s) 232, 1030
AcoI YGGCCR 1 cut(s) 1174
AcsI RAATTY 5 cut(s) 180, 551, 577, 588, 679
AcuI CTGAAG 3 cut(s) 382, 835, 1046
AfaI GTAC 2 cut(s) 44, 527
AfiI CCNNNNNNNGG 3 cut(s) 34, 489, 601
AgsI TTSAA 6 cut(s) 539, 632, 662, 763, 892, 1045
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 4 cut(s) 488, 520, 822, 854
AloI GAACNNNNNNTCC 2 cut(s) 1128, 1160
AluBI AGCT 3 cut(s) 331, 501, 927
AluI AGCT 3 cut(s) 331, 501, 927
Alw21I GWGCWC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 2 cut(s) 232, 1030
AoxI GGCC 5 cut(s) 128, 491, 603, 881, 1174
ApaI GGGCCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 244, 558
ApoI RAATTY 5 cut(s) 180, 551, 577, 588, 679
ArsI GACNNNNNNTTYG 2 cut(s) 1014, 1046
Asp718I GGTACC 2 cut(s) 42, 525
AspLEI GCGC 1 cut(s) 1130
AspS9I GGNCC 4 cut(s) 128, 129, 492, 943
AsuC2I CCSGG 1 cut(s) 931
AvaII GGWCC 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 996
BaeGI GKGCMC 3 cut(s) 132, 346, 906
BanI GGYRCC 2 cut(s) 42, 525
BanII GRGCYC 1 cut(s) 132
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
BbsI GAAGAC 2 cut(s) 669, 899
Bbv12I GWGCWC 1 cut(s) 346
BbvCI CCTCAGC 1 cut(s) 1124
BbvI GCAGC 2 cut(s) 256, 545
BccI CCATC 4 cut(s) 158, 538, 541, 1162
BceAI ACGGC 2 cut(s) 506, 1189
BciT130I CCWGG 1 cut(s) 885
BcnI CCSGG 1 cut(s) 931
BfaI CTAG 2 cut(s) 924, 1095
BfoI RGCGCY 1 cut(s) 1131
BglII AGATCT 2 cut(s) 989, 1106
BisI GCNGC 2 cut(s) 245, 559
BlsI GCNGC 2 cut(s) 246, 560
Bme1390I CCNGG 2 cut(s) 885, 931
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 4 cut(s) 128, 129, 492, 943
BmiI GGNNCC 4 cut(s) 44, 130, 527, 944
BmrFI CCNGG 2 cut(s) 885, 931
BmsI GCATC 5 cut(s) 14, 154, 311, 377, 652
BpiI GAAGAC 2 cut(s) 669, 899
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 1124
BpuEI CTTGAG 1 cut(s) 296
BpuMI CCSGG 1 cut(s) 931
Bsa29I ATCGAT 1 cut(s) 22
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 767
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 489, 601
Bse1I ACTGG 6 cut(s) 143, 341, 441, 816, 868, 946
Bse21I CCTNAGG 1 cut(s) 996
Bse3DI GCAATG 2 cut(s) 297, 478
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 767
BseGI GGATG 1 cut(s) 530
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 3 cut(s) 34, 489, 601
BseMI GCAATG 2 cut(s) 297, 478
BseMII CTCAG 3 cut(s) 397, 987, 1138
BseNI ACTGG 6 cut(s) 143, 341, 441, 816, 868, 946
BseRI GAGGAG 4 cut(s) 209, 475, 1040, 1139
BseSI GKGCMC 3 cut(s) 132, 346, 906
BseXI GCAGC 2 cut(s) 256, 545
BseYI CCCAGC 1 cut(s) 939
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 5 cut(s) 130, 493, 605, 883, 1176
BshNI GGYRCC 2 cut(s) 42, 525
BshVI ATCGAT 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 930
BslFI GGGAC 2 cut(s) 391, 956
BslI CCNNNNNNNGG 3 cut(s) 34, 489, 601
BsmFI GGGAC 2 cut(s) 391, 956
BsnI GGCC 5 cut(s) 130, 493, 605, 883, 1176
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 132, 346, 906
Bsp143I GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
BspACI CCGC 2 cut(s) 556, 1197
BspANI GGCC 5 cut(s) 130, 493, 605, 883, 1176
BspCNI CTCAG 3 cut(s) 396, 988, 1137
BspDI ATCGAT 1 cut(s) 22
BspFNI CGCG 1 cut(s) 600
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 4 cut(s) 44, 130, 527, 944
BspPI GGATC 2 cut(s) 232, 1030
BspQI GCTCTTC 1 cut(s) 397
BspT107I GGYRCC 2 cut(s) 42, 525
BsrDI GCAATG 2 cut(s) 297, 478
BsrI ACTGG 6 cut(s) 143, 341, 441, 816, 868, 946
BssECI CCNNGG 1 cut(s) 767
BssMI GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
BssT1I CCWWGG 1 cut(s) 767
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 1 cut(s) 414
Bst6I CTCTTC 2 cut(s) 397, 963
BstAPI GCANNNNNTGC 1 cut(s) 1109
BstDEI CTNAG 4 cut(s) 48, 383, 996, 1124
BstF5I GGATG 1 cut(s) 530
BstFNI CGCG 1 cut(s) 600
BstH2I RGCGCY 1 cut(s) 1131
BstHHI GCGC 1 cut(s) 1130
BstKTI GATC 6 cut(s) 227, 425, 992, 1006, 1038, 1109
BstMBI GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
BstMWI GCNNNNNNNGC 3 cut(s) 253, 1007, 1109
BstNI CCWGG 1 cut(s) 885
BstSCI CCNGG 2 cut(s) 883, 929
BstSLI GKGCMC 3 cut(s) 132, 346, 906
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 256, 545
BstV2I GAAGAC 2 cut(s) 669, 899
BstX2I RGATCY 4 cut(s) 224, 989, 1035, 1106
BstYI RGATCY 4 cut(s) 224, 989, 1035, 1106
Bsu15I ATCGAT 1 cut(s) 22
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 5 cut(s) 130, 493, 605, 883, 1176
BsuTUI ATCGAT 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 530
BtsI GCAGTG 2 cut(s) 782, 906
BtsIMutI CAGTG 6 cut(s) 406, 410, 434, 782, 875, 906
CciI TCATGA 1 cut(s) 82
CfoI GCGC 1 cut(s) 1130
Cfr13I GGNCC 4 cut(s) 128, 129, 492, 943
ClaI ATCGAT 1 cut(s) 22
CseI GACGC 1 cut(s) 968
Csp6I GTAC 2 cut(s) 43, 526
CviAII CATG 4 cut(s) 83, 443, 455, 801
CviQI GTAC 2 cut(s) 43, 526
DdeI CTNAG 4 cut(s) 48, 383, 996, 1124
DpnI GATC 6 cut(s) 226, 424, 991, 1005, 1037, 1108
DpnII GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
DraI TTTAAA 1 cut(s) 55
EaeI YGGCCR 1 cut(s) 1174
Eam1104I CTCTTC 2 cut(s) 397, 963
EarI CTCTTC 2 cut(s) 397, 963
Eco130I CCWWGG 1 cut(s) 767
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 3 cut(s) 382, 835, 1046
Eco81I CCTNAGG 1 cut(s) 996
EcoO109I RGGNCCY 2 cut(s) 128, 129
EcoRI GAATTC 2 cut(s) 180, 551
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 767
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 767
FaeI CATG 4 cut(s) 86, 446, 458, 804
FaiI YATR 9 cut(s) 84, 186, 444, 456, 462, 674, 726, 755, 802
FalI AAGNNNNNCTT 4 cut(s) 488, 520, 630, 662
FaqI GGGAC 2 cut(s) 391, 956
FatI CATG 4 cut(s) 82, 442, 454, 800
FauI CCCGC 1 cut(s) 1190
Fnu4HI GCNGC 2 cut(s) 245, 559
FokI GGATG 1 cut(s) 517
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 2 cut(s) 245, 559
FspBI CTAG 2 cut(s) 924, 1095
GlaI GCGC 1 cut(s) 1129
GluI GCNGC 2 cut(s) 245, 559
GsaI CCCAGC 1 cut(s) 943
GsuI CTGGAG 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 1131
HaeIII GGCC 5 cut(s) 130, 493, 605, 883, 1176
HapII CCGG 1 cut(s) 930
HgaI GACGC 1 cut(s) 968
HhaI GCGC 1 cut(s) 1130
Hin1II CATG 4 cut(s) 86, 446, 458, 804
Hin6I GCGC 1 cut(s) 1128
HinP1I GCGC 1 cut(s) 1128
HpaII CCGG 1 cut(s) 930
Hpy166II GTNNAC 3 cut(s) 344, 798, 847
Hpy8I GTNNAC 3 cut(s) 344, 798, 847
Hpy99I CGWCG 2 cut(s) 256, 1193
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4V TGCA 7 cut(s) 302, 344, 410, 442, 471, 1103, 1112
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 1007, 1109
HpyF3I CTNAG 4 cut(s) 48, 383, 996, 1124
Hsp92II CATG 4 cut(s) 86, 446, 458, 804
HspAI GCGC 1 cut(s) 1128
KpnI GGTACC 2 cut(s) 46, 529
Kzo9I GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
LguI GCTCTTC 1 cut(s) 397
LmnI GCTCC 2 cut(s) 773, 932
Lsp1109I GCAGC 2 cut(s) 256, 545
LweI GCATC 5 cut(s) 14, 154, 311, 377, 652
MaeI CTAG 2 cut(s) 924, 1095
MaeIII GTNAC 3 cut(s) 727, 972, 1202
MalI GATC 6 cut(s) 226, 424, 991, 1005, 1037, 1108
MboI GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
MflI RGATCY 4 cut(s) 224, 989, 1035, 1106
MhlI GDGCHC 3 cut(s) 132, 346, 906
MluCI AATT 7 cut(s) 180, 551, 577, 588, 679, 706, 855
MlyI GAGTC 5 cut(s) 245, 373, 425, 707, 1007
MmeI TCCRAC 4 cut(s) 261, 565, 1078, 1212
MseI TTAA 4 cut(s) 54, 288, 683, 747
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 2 cut(s) 558, 1197
MspI CCGG 1 cut(s) 930
MspR9I CCNGG 2 cut(s) 885, 931
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 3 cut(s) 253, 1007, 1109
NciI CCSGG 1 cut(s) 931
NdeII GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
NlaIII CATG 4 cut(s) 86, 446, 458, 804
NlaIV GGNNCC 4 cut(s) 44, 130, 527, 944
NmuCI GTSAC 2 cut(s) 727, 1202
PagI TCATGA 1 cut(s) 82
PciSI GCTCTTC 1 cut(s) 397
PfeI GAWTC 8 cut(s) 19, 79, 95, 122, 293, 353, 464, 571
PfoI TCCNGGA 1 cut(s) 929
PkrI GCNGC 2 cut(s) 246, 560
PleI GAGTC 5 cut(s) 244, 373, 425, 706, 1007
PpsI GAGTC 5 cut(s) 244, 373, 425, 706, 1007
Psp6I CCWGG 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 939
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 4 cut(s) 44, 130, 527, 944
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 4 cut(s) 128, 129, 492, 943
PsuI RGATCY 4 cut(s) 224, 989, 1035, 1106
RsaI GTAC 2 cut(s) 44, 527
RsaNI GTAC 2 cut(s) 43, 526
RseI CAYNNNNRTG 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 397
SaqAI TTAA 4 cut(s) 54, 288, 683, 747
SatI GCNGC 2 cut(s) 245, 559
Sau3AI GATC 6 cut(s) 224, 422, 989, 1003, 1035, 1106
Sau96I GGNCC 4 cut(s) 128, 129, 492, 943
SchI GAGTC 5 cut(s) 245, 373, 425, 707, 1007
ScrFI CCNGG 2 cut(s) 885, 931
SduI GDGCHC 3 cut(s) 132, 346, 906
SfaNI GCATC 5 cut(s) 14, 154, 311, 377, 652
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 7 cut(s) 180, 551, 577, 588, 679, 706, 855
SsiI CCGC 2 cut(s) 556, 1197
SspMI CTAG 2 cut(s) 924, 1095
StyD4I CCNGG 2 cut(s) 883, 929
StyI CCWWGG 1 cut(s) 767
TaaI ACNGT 1 cut(s) 414
TaqI TCGA 2 cut(s) 22, 1032
TasI AATT 7 cut(s) 180, 551, 577, 588, 679, 706, 855
TfiI GAWTC 8 cut(s) 19, 79, 95, 122, 293, 353, 464, 571
Tru1I TTAA 4 cut(s) 54, 288, 683, 747
Tru9I TTAA 4 cut(s) 54, 288, 683, 747
TscAI CASTG 6 cut(s) 406, 417, 441, 789, 875, 906
TseFI GTSAC 2 cut(s) 727, 1202
TseI GCWGC 2 cut(s) 244, 558
Tsp45I GTSAC 2 cut(s) 727, 1202
TspDTI ATGAA 7 cut(s) 17, 71, 99, 173, 174, 843, 981
TspGWI ACGGA 4 cut(s) 49, 91, 506, 1028
TspRI CASTG 6 cut(s) 406, 417, 441, 789, 875, 906
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 5 cut(s) 180, 551, 577, 588, 679
XspI CTAG 2 cut(s) 924, 1095
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.