RchiOBHm_Chr5g0052651

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003334
Physical Location & Seq
Reverse (-)
54638819 .. 54643415
4597 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33004

Sequence Viewer

Length: 870 bp
ATGAAGGAAGACGAAGTGAATCGATGCCAGATACAGGAATGGTACCGTAAGTTTAAATCCGTGTCTATCAAAACACGGATTCATGAACTTCCAGAATCCTTTGTCCAATATCTTCTTGATGATTCCAGGCCCTTCCTTCTTCCAGTTTCCATCTCAAATGAAGATGCCTTACCCAACAGAATTCATAATCCAGAGGAGGAAGATGACTATCAAGTAGTAGAAGGATCTAATGATGAGTCAGAGCAGCCTTCTTTGCCCCCTTCTTTTCCTAAACTTGAGTTGGAGATTAAGGAATCCATTGCATCGCTTGGAGGTGCTGTGTTCCCGAAGCTAAACTGGAGTGCACCAAAAGATTCTGCTTGGATTAGCACAACTGGGACTCTTAGATGCTCTTCCATCAGTGAGATTGTACTGTTGCTACGATCATCTGACTCACTGGTGCATGATTTGTGTCATGCTTATGATTCCTGCAATGACAAGACCTCCTCACGGCCCAAGAGCTTCTTCCTTGCACTCCGTAAATGGACCCAATCCCTTCAGCCAGAGATGGAATTCTGCTGCTTTGTGAGGAATCAGAATTTAGTTGGAATTTCCCAGCGCGAGGCCACAACCTTCTACCCTTCTCTGCTTGAAAGCAAAGAGCATCTGGGAGAGTTGATTGAAGAGTTCTTTATGGAAAATTTAAGCCCACCGGAGCCTGTAGGTAGCTACGCCTTTGATGTATACGTGATGAAGGACGAGCGAGTTAAGGTTATGGATTTCAACCCTTGGGGTGCATTTACACTGCCTCTGATGTTTACATGGGAGAAACTGGAACAGAGTGATAATGAACAGGGGGATGGTGTGGACTTCAGAATTGTGGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

289

Amino Acids

33.22

Weight (kDa)

4.63

Isoelectric Point (pI)

55.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 14 - 288 1.3e-89 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 42
AccB1I GGYRCC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 723
AccII CGCG 1 cut(s) 600
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 5 cut(s) 180, 551, 577, 588, 679
AcuI CTGAAG 2 cut(s) 521, 835
AfaI GTAC 2 cut(s) 44, 411
AfiI CCNNNNNNNGG 3 cut(s) 34, 489, 601
AgsI TTSAA 3 cut(s) 632, 662, 763
AjnI CCWGG 1 cut(s) 125
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 4 cut(s) 331, 501, 708, 867
AluI AGCT 4 cut(s) 331, 501, 708, 867
Alw21I GWGCWC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 232
AoxI GGCC 3 cut(s) 128, 491, 603
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 244, 558
ApoI RAATTY 5 cut(s) 180, 551, 577, 588, 679
Asp718I GGTACC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 600
AspS9I GGNCC 3 cut(s) 129, 492, 525
AvaII GGWCC 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 42
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 256, 545
BccI CCATC 4 cut(s) 158, 404, 541, 833
BceAI ACGGC 1 cut(s) 506
BciT130I CCWGG 1 cut(s) 127
BfaI CTAG 1 cut(s) 868
BfmI CTRYAG 1 cut(s) 699
BisI GCNGC 2 cut(s) 245, 559
BlsI GCNGC 2 cut(s) 246, 560
Bme1390I CCNGG 1 cut(s) 127
Bme18I GGWCC 1 cut(s) 525
BmgT120I GGNCC 3 cut(s) 129, 492, 525
BmiI GGNNCC 3 cut(s) 44, 527, 696
BmrFI CCNGG 1 cut(s) 127
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 5 cut(s) 14, 154, 311, 377, 652
BmuI ACTGGG 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 15
BpmI CTGGAG 1 cut(s) 358
BpuEI CTTGAG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 727
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 767
BsaWI WCCGGW 1 cut(s) 691
BsaXI ACNNNNNCTCC 4 cut(s) 303, 333, 467, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 489, 601
Bse1I ACTGG 5 cut(s) 143, 341, 379, 441, 816
Bse3DI GCAATG 2 cut(s) 297, 478
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 1 cut(s) 127
BseCI ATCGAT 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 767
BseGI GGATG 1 cut(s) 844
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 3 cut(s) 34, 489, 601
BseMI GCAATG 2 cut(s) 297, 478
BseNI ACTGG 5 cut(s) 143, 341, 379, 441, 816
BseRI GAGGAG 2 cut(s) 209, 475
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 2 cut(s) 256, 545
BseYI CCCAGC 1 cut(s) 594
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 3 cut(s) 130, 493, 605
BshNI GGYRCC 1 cut(s) 42
BshVI ATCGAT 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 346
BsiSI CCGG 1 cut(s) 692
BslFI GGGAC 1 cut(s) 391
BslI CCNNNNNNNGG 3 cut(s) 34, 489, 601
BsmFI GGGAC 1 cut(s) 391
BsnI GGCC 3 cut(s) 130, 493, 605
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 2 cut(s) 224, 422
BspANI GGCC 3 cut(s) 130, 493, 605
BspDI ATCGAT 1 cut(s) 22
BspFNI CGCG 1 cut(s) 600
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 3 cut(s) 44, 527, 696
BspPI GGATC 1 cut(s) 232
BspQI GCTCTTC 1 cut(s) 397
BspT107I GGYRCC 1 cut(s) 42
BsrDI GCAATG 2 cut(s) 297, 478
BsrI ACTGG 5 cut(s) 143, 341, 379, 441, 816
BssECI CCNNGG 1 cut(s) 767
BssMI GATC 2 cut(s) 224, 422
BssNAI GTATAC 1 cut(s) 724
BssT1I CCWWGG 1 cut(s) 767
Bst1107I GTATAC 1 cut(s) 724
Bst2UI CCWGG 1 cut(s) 127
Bst4CI ACNGT 2 cut(s) 47, 414
Bst6I CTCTTC 2 cut(s) 397, 657
BstBAI YACGTR 1 cut(s) 727
BstDEI CTNAG 1 cut(s) 383
BstF5I GGATG 1 cut(s) 844
BstFNI CGCG 1 cut(s) 600
BstHHI GCGC 1 cut(s) 600
BstKTI GATC 2 cut(s) 227, 425
BstMBI GATC 2 cut(s) 224, 422
BstMWI GCNNNNNNNGC 1 cut(s) 253
BstNI CCWGG 1 cut(s) 127
BstSCI CCNGG 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 699
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 256, 545
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 724
Bsu15I ATCGAT 1 cut(s) 22
BsuRI GGCC 3 cut(s) 130, 493, 605
BsuTUI ATCGAT 1 cut(s) 22
BtgZI GCGATG 1 cut(s) 288
BtsCI GGATG 1 cut(s) 844
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 3 cut(s) 406, 434, 782
CciI TCATGA 1 cut(s) 82
CfoI GCGC 1 cut(s) 600
Cfr13I GGNCC 3 cut(s) 129, 492, 525
ClaI ATCGAT 1 cut(s) 22
Csp6I GTAC 2 cut(s) 43, 410
CviAII CATG 4 cut(s) 83, 443, 455, 801
CviQI GTAC 2 cut(s) 43, 410
DdeI CTNAG 1 cut(s) 383
DpnI GATC 2 cut(s) 226, 424
DpnII GATC 2 cut(s) 224, 422
DraI TTTAAA 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 397, 657
EarI CTCTTC 2 cut(s) 397, 657
Eco130I CCWWGG 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 525
Eco57I CTGAAG 2 cut(s) 521, 835
EcoO109I RGGNCCY 1 cut(s) 129
EcoRI GAATTC 2 cut(s) 180, 551
EcoRII CCWGG 1 cut(s) 125
EcoT14I CCWWGG 1 cut(s) 767
ErhI CCWWGG 1 cut(s) 767
FaeI CATG 4 cut(s) 86, 446, 458, 804
FaiI YATR 9 cut(s) 84, 186, 444, 456, 462, 674, 724, 755, 802
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 1 cut(s) 391
FatI CATG 4 cut(s) 82, 442, 454, 800
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 2 cut(s) 245, 559
FokI GGATG 1 cut(s) 851
Fsp4HI GCNGC 2 cut(s) 245, 559
FspBI CTAG 1 cut(s) 868
GlaI GCGC 1 cut(s) 599
GluI GCNGC 2 cut(s) 245, 559
GsaI CCCAGC 1 cut(s) 598
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 3 cut(s) 130, 493, 605
HapII CCGG 1 cut(s) 692
HhaI GCGC 1 cut(s) 600
Hin1II CATG 4 cut(s) 86, 446, 458, 804
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
HpaII CCGG 1 cut(s) 692
Hpy166II GTNNAC 4 cut(s) 344, 724, 798, 847
Hpy188I TCNGA 5 cut(s) 241, 430, 576, 792, 854
Hpy188III TCNNGA 5 cut(s) 83, 92, 116, 191, 325
Hpy8I GTNNAC 4 cut(s) 344, 724, 798, 847
HpyAV CCTTC 9 cut(s) 142, 146, 215, 258, 270, 545, 622, 630, 727
HpyCH4III ACNGT 2 cut(s) 47, 414
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 6 cut(s) 302, 344, 442, 471, 512, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 253
HpyF3I CTNAG 1 cut(s) 383
HpySE526I ACGT 1 cut(s) 726
Hsp92II CATG 4 cut(s) 86, 446, 458, 804
HspAI GCGC 1 cut(s) 598
KpnI GGTACC 1 cut(s) 46
Kzo9I GATC 2 cut(s) 224, 422
LguI GCTCTTC 1 cut(s) 397
LmnI GCTCC 1 cut(s) 694
Lsp1109I GCAGC 2 cut(s) 256, 545
LweI GCATC 5 cut(s) 14, 154, 311, 377, 652
MaeI CTAG 1 cut(s) 868
MaeII ACGT 1 cut(s) 726
MalI GATC 2 cut(s) 226, 424
MboI GATC 2 cut(s) 224, 422
MboII GAAGA 8 cut(s) 20, 104, 131, 173, 212, 384, 496, 674
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 346
MluCI AATT 6 cut(s) 180, 551, 577, 588, 679, 855
MlyI GAGTC 3 cut(s) 245, 373, 425
MmeI TCCRAC 2 cut(s) 261, 565
MnlI CCTC 8 cut(s) 187, 190, 305, 493, 496, 561, 595, 798
MseI TTAA 4 cut(s) 54, 288, 683, 747
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 1 cut(s) 692
MspR9I CCNGG 1 cut(s) 127
MvaI CCWGG 1 cut(s) 127
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 1 cut(s) 253
NdeII GATC 2 cut(s) 224, 422
NlaIII CATG 4 cut(s) 86, 446, 458, 804
NlaIV GGNNCC 3 cut(s) 44, 527, 696
PagI TCATGA 1 cut(s) 82
PciSI GCTCTTC 1 cut(s) 397
PfeI GAWTC 8 cut(s) 19, 79, 95, 122, 293, 353, 464, 571
PkrI GCNGC 2 cut(s) 246, 560
PleI GAGTC 3 cut(s) 244, 373, 425
PpsI GAGTC 3 cut(s) 244, 373, 425
Ppu21I YACGTR 1 cut(s) 727
Psp6I CCWGG 1 cut(s) 125
PspFI CCCAGC 1 cut(s) 594
PspGI CCWGG 1 cut(s) 125
PspN4I GGNNCC 3 cut(s) 44, 527, 696
PspPI GGNCC 3 cut(s) 129, 492, 525
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 2 cut(s) 44, 411
RsaNI GTAC 2 cut(s) 43, 410
RseI CAYNNNNRTG 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 397
SaqAI TTAA 4 cut(s) 54, 288, 683, 747
SatI GCNGC 2 cut(s) 245, 559
Sau3AI GATC 2 cut(s) 224, 422
Sau96I GGNCC 3 cut(s) 129, 492, 525
SchI GAGTC 3 cut(s) 245, 373, 425
ScrFI CCNGG 1 cut(s) 127
SduI GDGCHC 1 cut(s) 346
SfaNI GCATC 5 cut(s) 14, 154, 311, 377, 652
SfcI CTRYAG 1 cut(s) 699
SinI GGWCC 1 cut(s) 525
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 6 cut(s) 180, 551, 577, 588, 679, 855
SspMI CTAG 1 cut(s) 868
StyD4I CCNGG 1 cut(s) 125
StyI CCWWGG 1 cut(s) 767
TaaI ACNGT 2 cut(s) 47, 414
TaiI ACGT 1 cut(s) 729
TaqI TCGA 1 cut(s) 22
TasI AATT 6 cut(s) 180, 551, 577, 588, 679, 855
TatI WGTACW 1 cut(s) 409
TfiI GAWTC 8 cut(s) 19, 79, 95, 122, 293, 353, 464, 571
Tru1I TTAA 4 cut(s) 54, 288, 683, 747
Tru9I TTAA 4 cut(s) 54, 288, 683, 747
TscAI CASTG 3 cut(s) 406, 441, 789
TseI GCWGC 2 cut(s) 244, 558
TspDTI ATGAA 7 cut(s) 17, 71, 99, 173, 174, 746, 843
TspGWI ACGGA 3 cut(s) 49, 91, 506
TspRI CASTG 3 cut(s) 406, 441, 789
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 525
XapI RAATTY 5 cut(s) 180, 551, 577, 588, 679
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 1 cut(s) 868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.