Rh5BG353300

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0003334
Physical Location & Seq
Forward (+)
54684296 .. 54685294
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG353300.1

Sequence Viewer

Length: 999 bp
ATGAAGGAAGAAGAAGTGAATCGATGCCAGATACAGGAATGGTACCCTAAGTTTAAATCCGTGTCTATCAAAACACGGATTCATGAACTTCCAGAATCCTTTGTCCAATATCTTCTTGATGATTCAGGCCCCTTCCTTCTTCCAGTTTCCATCTCAAATGAAGATGCCTTACCCAACAGAATTCATAATCCAGAGGAGGAAGATGACTATCAAGTAATAGAAGGATCTGATGATGAGTCAGAGCAGCCTTCTTTGCCCCCTTCTTTTCCTGAACTTGAGTTGGAGATTAAGGAATCCATTGCATCACTTGGAGGTGCTGTGTTCCCGAAGCTAAACTGGAGTGCACCAAAAGATTCTGCTTGGATTAGCACAACTGGGACTCTCAGATGCTCTTCCTTCAGTGAGATTGCACTGTTGCTACGATCATCTGACTCACTGGTGCATGATTTGTGTCATGCTTATGATTCCTGCAATGACAAGACCTCCTCACGGCCCAAGAGCTTCTTCCTTGCTCTCCGTAAATGGTACCCATCCCTTCAGCCAGAGATGGAATTCCGCTGCTTTGTGAGGAATCAGAATTTAGTTGGAATTTCCCAGCGCGAGGCCACAACCTTCTACCCTTCTCTGCTTAAAAGCAAAGAGCATCTGGGAGAGTTGATCGAAGAGTTCTTTATGGAAAATTTAAGGTCAAGATTCGAGTCAGAAAATTATACCTTTGATGTATACGTGACGAAGGACAAGCGAGTTAAGGTTATGGATTTCAACCCTTGGGGTGCATTTACACTGCCTCTGATGTTTACATGGGAGGAACTGGAACAGAGTGATAATGAACAAGGGGATGGTGTGGACTTCAGAATTGTGGAGAGCCAGTGTGGTGTTAGGCCAGGTTTGAAGACAGCAGTGCCCTACGATTATGTGGACACTAGCTCTGGGAGTGGCTGGGACCAGTTTTTCAGGAATGCCGATAAAGAGTTACAGCGGCAAAGTAGGTCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

332

Amino Acids

38.27

Weight (kDa)

4.7

Isoelectric Point (pI)

61.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 14 - 309 1.4e-99 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 42, 525
AccB1I GGYRCC 2 cut(s) 42, 525
AccI GTMKAC 1 cut(s) 723
AccII CGCG 1 cut(s) 600
AciI CCGC 2 cut(s) 556, 979
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 5 cut(s) 180, 551, 577, 588, 679
AcuI CTGAAG 3 cut(s) 382, 521, 835
AfaI GTAC 2 cut(s) 44, 527
AfiI CCNNNNNNNGG 3 cut(s) 34, 489, 601
AgsI TTSAA 2 cut(s) 763, 892
AjnI CCWGG 1 cut(s) 883
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 3 cut(s) 331, 501, 927
AluI AGCT 3 cut(s) 331, 501, 927
Alw21I GWGCWC 1 cut(s) 346
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 232
AoxI GGCC 4 cut(s) 127, 491, 603, 881
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 244, 558
ApoI RAATTY 5 cut(s) 180, 551, 577, 588, 679
Asp718I GGTACC 2 cut(s) 42, 525
AspLEI GCGC 1 cut(s) 600
AspS9I GGNCC 3 cut(s) 128, 492, 943
AvaII GGWCC 1 cut(s) 943
BaeGI GKGCMC 2 cut(s) 346, 906
BanI GGYRCC 2 cut(s) 42, 525
BarI GAAGNNNNNNTAC 2 cut(s) 153, 185
BbsI GAAGAC 1 cut(s) 899
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 2 cut(s) 256, 545
BccI CCATC 4 cut(s) 158, 538, 541, 833
BceAI ACGGC 1 cut(s) 506
BciT130I CCWGG 1 cut(s) 885
BfaI CTAG 1 cut(s) 924
BisI GCNGC 3 cut(s) 245, 559, 980
BlsI GCNGC 3 cut(s) 246, 560, 981
Bme1390I CCNGG 1 cut(s) 885
Bme18I GGWCC 1 cut(s) 943
BmgT120I GGNCC 3 cut(s) 128, 492, 943
BmiI GGNNCC 4 cut(s) 44, 130, 527, 944
BmrFI CCNGG 1 cut(s) 885
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 5 cut(s) 14, 154, 311, 377, 652
BmuI ACTGGG 1 cut(s) 384
BpiI GAAGAC 1 cut(s) 899
BpmI CTGGAG 1 cut(s) 358
BpuEI CTTGAG 1 cut(s) 296
Bsa29I ATCGAT 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 727
BsaBI GATNNNNATC 1 cut(s) 207
BsaJI CCNNGG 1 cut(s) 767
BsaXI ACNNNNNCTCC 6 cut(s) 303, 333, 467, 497, 854, 884
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 489, 601
Bse1I ACTGG 7 cut(s) 143, 341, 379, 441, 816, 868, 946
Bse3DI GCAATG 2 cut(s) 297, 478
Bse8I GATNNNNATC 1 cut(s) 207
BseBI CCWGG 1 cut(s) 885
BseCI ATCGAT 1 cut(s) 22
BseDI CCNNGG 1 cut(s) 767
BseGI GGATG 2 cut(s) 530, 844
BseJI GATNNNNATC 1 cut(s) 207
BseLI CCNNNNNNNGG 3 cut(s) 34, 489, 601
BseMI GCAATG 2 cut(s) 297, 478
BseMII CTCAG 1 cut(s) 397
BseNI ACTGG 7 cut(s) 143, 341, 379, 441, 816, 868, 946
BseRI GAGGAG 2 cut(s) 209, 475
BseSI GKGCMC 2 cut(s) 346, 906
BseXI GCAGC 2 cut(s) 256, 545
BseYI CCCAGC 2 cut(s) 594, 939
Bsh1236I CGCG 1 cut(s) 600
BshFI GGCC 4 cut(s) 129, 493, 605, 883
BshNI GGYRCC 2 cut(s) 42, 525
BshVI ATCGAT 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 346
BslFI GGGAC 2 cut(s) 391, 956
BslI CCNNNNNNNGG 3 cut(s) 34, 489, 601
BsmFI GGGAC 2 cut(s) 391, 956
BsmI GAATGC 1 cut(s) 964
BsnI GGCC 4 cut(s) 129, 493, 605, 883
Bsp1286I GDGCHC 2 cut(s) 346, 906
Bsp143I GATC 3 cut(s) 224, 422, 657
BspACI CCGC 2 cut(s) 556, 979
BspANI GGCC 4 cut(s) 129, 493, 605, 883
BspCNI CTCAG 1 cut(s) 396
BspDI ATCGAT 1 cut(s) 22
BspFNI CGCG 1 cut(s) 600
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 4 cut(s) 44, 130, 527, 944
BspPI GGATC 1 cut(s) 232
BspQI GCTCTTC 1 cut(s) 397
BspT107I GGYRCC 2 cut(s) 42, 525
BsrDI GCAATG 2 cut(s) 297, 478
BsrI ACTGG 7 cut(s) 143, 341, 379, 441, 816, 868, 946
BssECI CCNNGG 1 cut(s) 767
BssMI GATC 3 cut(s) 224, 422, 657
BssNAI GTATAC 1 cut(s) 724
BssT1I CCWWGG 1 cut(s) 767
Bst1107I GTATAC 1 cut(s) 724
Bst2UI CCWGG 1 cut(s) 885
Bst4CI ACNGT 1 cut(s) 414
Bst6I CTCTTC 2 cut(s) 397, 657
BstBAI YACGTR 1 cut(s) 727
BstDEI CTNAG 2 cut(s) 48, 383
BstF5I GGATG 2 cut(s) 530, 844
BstFNI CGCG 1 cut(s) 600
BstHHI GCGC 1 cut(s) 600
BstKTI GATC 3 cut(s) 227, 425, 660
BstMBI GATC 3 cut(s) 224, 422, 657
BstMWI GCNNNNNNNGC 1 cut(s) 253
BstNI CCWGG 1 cut(s) 885
BstSCI CCNGG 1 cut(s) 883
BstSLI GKGCMC 2 cut(s) 346, 906
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 2 cut(s) 256, 545
BstV2I GAAGAC 1 cut(s) 899
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 1 cut(s) 724
Bsu15I ATCGAT 1 cut(s) 22
BsuRI GGCC 4 cut(s) 129, 493, 605, 883
BsuTUI ATCGAT 1 cut(s) 22
BtsCI GGATG 2 cut(s) 530, 844
BtsI GCAGTG 2 cut(s) 782, 906
BtsIMutI CAGTG 6 cut(s) 406, 410, 434, 782, 875, 906
CciI TCATGA 1 cut(s) 82
CfoI GCGC 1 cut(s) 600
Cfr13I GGNCC 3 cut(s) 128, 492, 943
ClaI ATCGAT 1 cut(s) 22
Csp6I GTAC 2 cut(s) 43, 526
CviAII CATG 4 cut(s) 83, 443, 455, 801
CviQI GTAC 2 cut(s) 43, 526
DdeI CTNAG 2 cut(s) 48, 383
DpnI GATC 3 cut(s) 226, 424, 659
DpnII GATC 3 cut(s) 224, 422, 657
DraI TTTAAA 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 397, 657
EarI CTCTTC 2 cut(s) 397, 657
Eco130I CCWWGG 1 cut(s) 767
Eco47I GGWCC 1 cut(s) 943
Eco57I CTGAAG 3 cut(s) 382, 521, 835
EcoO109I RGGNCCY 1 cut(s) 128
EcoRI GAATTC 2 cut(s) 180, 551
EcoRII CCWGG 1 cut(s) 883
EcoT14I CCWWGG 1 cut(s) 767
ErhI CCWWGG 1 cut(s) 767
FaeI CATG 4 cut(s) 86, 446, 458, 804
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 2 cut(s) 391, 956
FatI CATG 4 cut(s) 82, 442, 454, 800
FblI GTMKAC 1 cut(s) 723
Fnu4HI GCNGC 3 cut(s) 245, 559, 980
FokI GGATG 2 cut(s) 517, 851
Fsp4HI GCNGC 3 cut(s) 245, 559, 980
FspBI CTAG 1 cut(s) 924
GlaI GCGC 1 cut(s) 599
GluI GCNGC 3 cut(s) 245, 559, 980
GsaI CCCAGC 2 cut(s) 598, 943
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 4 cut(s) 129, 493, 605, 883
HhaI GCGC 1 cut(s) 600
Hin1II CATG 4 cut(s) 86, 446, 458, 804
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
Hpy166II GTNNAC 5 cut(s) 344, 724, 798, 847, 919
Hpy188I TCNGA 8 cut(s) 229, 241, 386, 430, 576, 703, 792, 854
Hpy188III TCNNGA 8 cut(s) 83, 92, 116, 191, 269, 325, 690, 955
Hpy8I GTNNAC 5 cut(s) 344, 724, 798, 847, 919
HpyCH4III ACNGT 1 cut(s) 414
HpyCH4IV ACGT 1 cut(s) 726
HpyCH4V TGCA 6 cut(s) 302, 344, 410, 442, 471, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 253
HpyF3I CTNAG 2 cut(s) 48, 383
HpySE526I ACGT 1 cut(s) 726
Hsp92II CATG 4 cut(s) 86, 446, 458, 804
HspAI GCGC 1 cut(s) 598
KpnI GGTACC 2 cut(s) 46, 529
Kzo9I GATC 3 cut(s) 224, 422, 657
LguI GCTCTTC 1 cut(s) 397
Lsp1109I GCAGC 2 cut(s) 256, 545
LweI GCATC 5 cut(s) 14, 154, 311, 377, 652
MaeI CTAG 1 cut(s) 924
MaeII ACGT 1 cut(s) 726
MaeIII GTNAC 2 cut(s) 727, 972
MalI GATC 3 cut(s) 226, 424, 659
MboI GATC 3 cut(s) 224, 422, 657
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 2 cut(s) 346, 906
MluCI AATT 7 cut(s) 180, 551, 577, 588, 679, 706, 855
MlyI GAGTC 4 cut(s) 245, 373, 425, 707
MmeI TCCRAC 2 cut(s) 261, 565
MnlI CCTC 9 cut(s) 187, 190, 305, 493, 496, 561, 595, 798, 799
MseI TTAA 6 cut(s) 54, 288, 630, 683, 747, 997
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 2 cut(s) 558, 979
MspR9I CCNGG 1 cut(s) 885
Mva1269I GAATGC 1 cut(s) 964
MvaI CCWGG 1 cut(s) 885
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 1 cut(s) 253
NdeII GATC 3 cut(s) 224, 422, 657
NlaIII CATG 4 cut(s) 86, 446, 458, 804
NlaIV GGNNCC 4 cut(s) 44, 130, 527, 944
NmuCI GTSAC 1 cut(s) 727
PagI TCATGA 1 cut(s) 82
PciSI GCTCTTC 1 cut(s) 397
PctI GAATGC 1 cut(s) 964
PfeI GAWTC 9 cut(s) 19, 79, 95, 122, 293, 353, 464, 571, 693
PkrI GCNGC 3 cut(s) 246, 560, 981
PleI GAGTC 4 cut(s) 244, 373, 425, 706
PpsI GAGTC 4 cut(s) 244, 373, 425, 706
Ppu21I YACGTR 1 cut(s) 727
Psp6I CCWGG 1 cut(s) 883
PspFI CCCAGC 2 cut(s) 594, 939
PspGI CCWGG 1 cut(s) 883
PspN4I GGNNCC 4 cut(s) 44, 130, 527, 944
PspPI GGNCC 3 cut(s) 128, 492, 943
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 2 cut(s) 44, 527
RsaNI GTAC 2 cut(s) 43, 526
RseI CAYNNNNRTG 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 397
SaqAI TTAA 6 cut(s) 54, 288, 630, 683, 747, 997
SatI GCNGC 3 cut(s) 245, 559, 980
Sau3AI GATC 3 cut(s) 224, 422, 657
Sau96I GGNCC 3 cut(s) 128, 492, 943
SchI GAGTC 4 cut(s) 245, 373, 425, 707
ScrFI CCNGG 1 cut(s) 885
SduI GDGCHC 2 cut(s) 346, 906
SfaNI GCATC 5 cut(s) 14, 154, 311, 377, 652
SinI GGWCC 1 cut(s) 943
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 7 cut(s) 180, 551, 577, 588, 679, 706, 855
SsiI CCGC 2 cut(s) 556, 979
SspMI CTAG 1 cut(s) 924
StyD4I CCNGG 1 cut(s) 883
StyI CCWWGG 1 cut(s) 767
TaaI ACNGT 1 cut(s) 414
TaiI ACGT 1 cut(s) 729
TaqI TCGA 3 cut(s) 22, 660, 696
TasI AATT 7 cut(s) 180, 551, 577, 588, 679, 706, 855
TauI GCSGC 1 cut(s) 982
TfiI GAWTC 9 cut(s) 19, 79, 95, 122, 293, 353, 464, 571, 693
Tru1I TTAA 6 cut(s) 54, 288, 630, 683, 747, 997
Tru9I TTAA 6 cut(s) 54, 288, 630, 683, 747, 997
TscAI CASTG 6 cut(s) 406, 417, 441, 789, 875, 906
TseFI GTSAC 1 cut(s) 727
TseI GCWGC 2 cut(s) 244, 558
Tsp45I GTSAC 1 cut(s) 727
TspDTI ATGAA 6 cut(s) 17, 71, 99, 173, 174, 843
TspGWI ACGGA 3 cut(s) 49, 91, 506
TspRI CASTG 6 cut(s) 406, 417, 441, 789, 875, 906
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 943
XapI RAATTY 5 cut(s) 180, 551, 577, 588, 679
XmiI GTMKAC 1 cut(s) 723
XspI CTAG 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.