pycom09g01300

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0003334
Physical Location & Seq
Forward (+)
964476 .. 965477
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01300.1

Sequence Viewer

Length: 1002 bp
ATGGAGGAGGAAGAAGTGAATCGATGCCAGATACAGGAATGGTACCCAAAGTTTAAATCTTTGTCTATCAAAACCCGAATTCATGAACTTCCGGAATCCTTTGTCCAATACCTTCTCGACGATTCAGGGCCCTTCCTTCTCCCTGTTTCGATCTCAAATGACGATGCATTCCCCAACAGAATTCATAATCCGGAGGAGGAAGACGACTATCAAGTATCAGAAGGGTCTGGTGATGAGTCCGAGCAGCCTTCTATGCCACCCTCTTTCCCCGAACTTGAGTTGGAGGTCAAGGAATCGATTAAGTCCCTTGGAGGTTCTGTGTTCCCTAAGCTAAACTGGAGTGCACCAAAAGACTCGGCTTGGATCAGCACAACTGGGAGCCTCAAGTGCTCTTCGTTCAGTGAGATTGCGCTCTTGCTACGATCATCTGACTCATTGGTCCATGATCTGTGTCACGCATATGATTCCTGCAGTGACAAAACCTCAACAAGGCCTAAGAGTTTCTTCCTTGCGCTACGGAAATGGTACCAGTCCCTCAAGCCAGAGATGGAGTTCCGTTGCTTTGTACGAAATCATAATTTAGTTGGAATTTCACAACGTGAGGTCACTACTTGTTATCCTGCTCTGCTTGAAAAGAAACACAGCCTTCAAGCGTTGATCGAAGACTTTTTCGTGGATAATTTGATGTCCAGATTTGAATCAGAAAATTACACCTTTGATGTATATGTGACACAGGATGATCGCATTAAGGTTGTGGATTTCAACCCATGGGGTGCATTTACGCTACCTCTAATGTTTACATGGGAGGAATTGGATCAGATTCGAGGGGATGGTGTTGAATTCAGAATTGTAGAGAGTCAGTTATCTGTTAGGCCAGGTCTGAAGACAGCCGTGCCGTTTGATTTTTTGGACACAAGTGCTGGGAGTGGCTGGGACCAAGTTATCAGAAATGCTGATGAAGAGTTGCAGCAGCAAACTAGGAACGGTCAAGCAGGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
D123

Protein Analysis

334

Amino Acids

38.07

Weight (kDa)

4.64

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
D123 PF07065 14 - 306 3e-95 D123
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 983
AasI GACNNNNNNGTC 1 cut(s) 437
Acc36I ACCTGC 1 cut(s) 983
Acc65I GGTACC 2 cut(s) 42, 525
AccB1I GGYRCC 2 cut(s) 42, 525
AccIII TCCGGA 2 cut(s) 91, 190
AclWI GGATC 2 cut(s) 371, 822
AcsI RAATTY 4 cut(s) 78, 180, 588, 839
AcuI CTGAAG 1 cut(s) 902
AdeI CACNNNGTG 1 cut(s) 599
AfaI GTAC 3 cut(s) 44, 527, 567
AfiI CCNNNNNNNGG 2 cut(s) 34, 489
AgsI TTSAA 5 cut(s) 632, 650, 698, 763, 839
AjnI CCWGG 1 cut(s) 874
AluBI AGCT 1 cut(s) 331
AluI AGCT 1 cut(s) 331
Alw21I GWGCWC 2 cut(s) 346, 392
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 2 cut(s) 371, 822
Aor13HI TCCGGA 2 cut(s) 91, 190
AoxI GGCC 3 cut(s) 128, 491, 872
ApaI GGGCCC 1 cut(s) 132
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 244, 967, 970
ApoI RAATTY 4 cut(s) 78, 180, 588, 839
Asp718I GGTACC 2 cut(s) 42, 525
AspLEI GCGC 2 cut(s) 412, 514
AspS9I GGNCC 4 cut(s) 128, 129, 439, 934
AsuHPI GGTGA 1 cut(s) 242
AvaII GGWCC 2 cut(s) 439, 934
BaeGI GKGCMC 2 cut(s) 132, 346
BanI GGYRCC 2 cut(s) 42, 525
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 3 cut(s) 207, 669, 890
Bbv12I GWGCWC 2 cut(s) 346, 392
BbvI GCAGC 3 cut(s) 256, 979, 982
BccI CCATC 2 cut(s) 541, 824
BceAI ACGGC 2 cut(s) 875, 880
BciT130I CCWGG 1 cut(s) 876
BfaI CTAG 1 cut(s) 978
BfmI CTRYAG 1 cut(s) 469
BfuAI ACCTGC 1 cut(s) 983
BisI GCNGC 3 cut(s) 245, 968, 971
BlsI GCNGC 3 cut(s) 246, 969, 972
Bme1390I CCNGG 1 cut(s) 876
Bme18I GGWCC 2 cut(s) 439, 934
BmgT120I GGNCC 4 cut(s) 128, 129, 439, 934
BmiI GGNNCC 5 cut(s) 44, 130, 380, 527, 935
BmrFI CCNGG 1 cut(s) 876
BmrI ACTGGG 1 cut(s) 384
BmsI GCATC 2 cut(s) 14, 154
BmuI ACTGGG 1 cut(s) 384
BpiI GAAGAC 3 cut(s) 207, 669, 890
BpmI CTGGAG 1 cut(s) 358
Bpu10I CCTNAGC 1 cut(s) 327
BpuEI CTTGAG 3 cut(s) 296, 368, 521
Bsa29I ATCGAT 2 cut(s) 22, 296
BsaBI GATNNNNATC 1 cut(s) 697
BsaJI CCNNGG 2 cut(s) 307, 767
BsaWI WCCGGW 2 cut(s) 91, 190
BsaXI ACNNNNNCTCC 2 cut(s) 303, 333
Bsc4I CCNNNNNNNGG 2 cut(s) 34, 489
Bse1I ACTGG 3 cut(s) 341, 379, 529
Bse8I GATNNNNATC 1 cut(s) 697
BseAI TCCGGA 2 cut(s) 91, 190
BseBI CCWGG 1 cut(s) 876
BseCI ATCGAT 2 cut(s) 22, 296
BseDI CCNNGG 2 cut(s) 307, 767
BseGI GGATG 2 cut(s) 742, 835
BseJI GATNNNNATC 1 cut(s) 697
BseLI CCNNNNNNNGG 2 cut(s) 34, 489
BseNI ACTGG 3 cut(s) 341, 379, 529
BseRI GAGGAG 2 cut(s) 20, 209
BseSI GKGCMC 2 cut(s) 132, 346
BseXI GCAGC 3 cut(s) 256, 979, 982
BseYI CCCAGC 2 cut(s) 920, 930
BshFI GGCC 3 cut(s) 130, 493, 874
BshNI GGYRCC 2 cut(s) 42, 525
BshVI ATCGAT 2 cut(s) 22, 296
BsiHKAI GWGCWC 2 cut(s) 346, 392
BsiSI CCGG 2 cut(s) 92, 191
BslFI GGGAC 3 cut(s) 289, 517, 947
BslI CCNNNNNNNGG 2 cut(s) 34, 489
BsmFI GGGAC 3 cut(s) 289, 517, 947
BsmI GAATGC 1 cut(s) 167
BsnI GGCC 3 cut(s) 130, 493, 874
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 132, 346, 392
Bsp13I TCCGGA 2 cut(s) 91, 190
Bsp143I GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
Bsp19I CCATGG 1 cut(s) 767
BspANI GGCC 3 cut(s) 130, 493, 874
BspDI ATCGAT 2 cut(s) 22, 296
BspEI TCCGGA 2 cut(s) 91, 190
BspHI TCATGA 1 cut(s) 82
BspLI GGNNCC 5 cut(s) 44, 130, 380, 527, 935
BspMAI CTGCAG 1 cut(s) 473
BspMI ACCTGC 1 cut(s) 983
BspPI GGATC 2 cut(s) 371, 822
BspQI GCTCTTC 1 cut(s) 397
BspT107I GGYRCC 2 cut(s) 42, 525
BsrI ACTGG 3 cut(s) 341, 379, 529
BssECI CCNNGG 2 cut(s) 307, 767
BssMI GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
BssT1I CCWWGG 2 cut(s) 307, 767
Bst2UI CCWGG 1 cut(s) 876
Bst4CI ACNGT 1 cut(s) 986
Bst6I CTCTTC 2 cut(s) 397, 954
BstDEI CTNAG 2 cut(s) 327, 495
BstDSI CCRYGG 1 cut(s) 767
BstENI CCTNNNNNAGG 1 cut(s) 487
BstF5I GGATG 2 cut(s) 742, 835
BstHHI GCGC 2 cut(s) 412, 514
BstKTI GATC 7 cut(s) 153, 366, 425, 448, 660, 742, 817
BstMBI GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
BstMWI GCNNNNNNNGC 2 cut(s) 253, 387
BstNI CCWGG 1 cut(s) 876
BstSCI CCNGG 1 cut(s) 874
BstSFI CTRYAG 1 cut(s) 469
BstSLI GKGCMC 2 cut(s) 132, 346
BstV1I GCAGC 3 cut(s) 256, 979, 982
BstV2I GAAGAC 3 cut(s) 207, 669, 890
Bsu15I ATCGAT 2 cut(s) 22, 296
BsuRI GGCC 3 cut(s) 130, 493, 874
BsuTUI ATCGAT 2 cut(s) 22, 296
BtgI CCRYGG 1 cut(s) 767
BtsCI GGATG 2 cut(s) 742, 835
BtsI GCAGTG 1 cut(s) 478
BtsIMutI CAGTG 2 cut(s) 406, 478
BveI ACCTGC 1 cut(s) 983
CciI TCATGA 1 cut(s) 82
CfoI GCGC 2 cut(s) 412, 514
Cfr13I GGNCC 4 cut(s) 128, 129, 439, 934
ClaI ATCGAT 2 cut(s) 22, 296
Csp6I GTAC 3 cut(s) 43, 526, 566
CviAII CATG 4 cut(s) 83, 443, 768, 801
CviQI GTAC 3 cut(s) 43, 526, 566
DdeI CTNAG 2 cut(s) 327, 495
DpnI GATC 7 cut(s) 152, 365, 424, 447, 659, 741, 816
DpnII GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
DraI TTTAAA 1 cut(s) 55
DraIII CACNNNGTG 1 cut(s) 599
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
Eam1104I CTCTTC 2 cut(s) 397, 954
EarI CTCTTC 2 cut(s) 397, 954
Eco130I CCWWGG 2 cut(s) 307, 767
Eco147I AGGCCT 1 cut(s) 493
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 439, 934
Eco57I CTGAAG 1 cut(s) 902
EcoNI CCTNNNNNAGG 1 cut(s) 487
EcoO109I RGGNCCY 2 cut(s) 128, 129
EcoRI GAATTC 3 cut(s) 78, 180, 839
EcoRII CCWGG 1 cut(s) 874
EcoT14I CCWWGG 2 cut(s) 307, 767
EcoT22I ATGCAT 1 cut(s) 169
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 307, 767
FaeI CATG 4 cut(s) 86, 446, 771, 804
FalI AAGNNNNNCTT 2 cut(s) 488, 520
FaqI GGGAC 3 cut(s) 289, 517, 947
FatI CATG 4 cut(s) 82, 442, 767, 800
FauNDI CATATG 1 cut(s) 460
Fnu4HI GCNGC 3 cut(s) 245, 968, 971
FokI GGATG 2 cut(s) 749, 842
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 3 cut(s) 245, 968, 971
FspBI CTAG 1 cut(s) 978
GlaI GCGC 2 cut(s) 411, 513
GluI GCNGC 3 cut(s) 245, 968, 971
GsaI CCCAGC 2 cut(s) 924, 934
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 3 cut(s) 130, 493, 874
HapII CCGG 2 cut(s) 92, 191
HhaI GCGC 2 cut(s) 412, 514
Hin1II CATG 4 cut(s) 86, 446, 771, 804
Hin6I GCGC 2 cut(s) 410, 512
HinP1I GCGC 2 cut(s) 410, 512
HpaII CCGG 2 cut(s) 92, 191
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 2 cut(s) 344, 798
Hpy188I TCNGA 8 cut(s) 220, 241, 430, 703, 819, 845, 882, 947
Hpy188III TCNNGA 5 cut(s) 83, 92, 116, 191, 690
Hpy8I GTNNAC 2 cut(s) 344, 798
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 6 cut(s) 122, 142, 146, 215, 258, 656
HpyCH4III ACNGT 1 cut(s) 986
HpyCH4IV ACGT 1 cut(s) 598
HpyCH4V TGCA 5 cut(s) 167, 344, 471, 776, 967
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 387
HpyF3I CTNAG 2 cut(s) 327, 495
HpySE526I ACGT 1 cut(s) 598
Hsp92II CATG 4 cut(s) 86, 446, 771, 804
HspAI GCGC 2 cut(s) 410, 512
Kpn2I TCCGGA 2 cut(s) 91, 190
KpnI GGTACC 2 cut(s) 46, 529
Kzo9I GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
LguI GCTCTTC 1 cut(s) 397
LmnI GCTCC 1 cut(s) 378
Lsp1109I GCAGC 3 cut(s) 256, 979, 982
LweI GCATC 2 cut(s) 14, 154
MaeI CTAG 1 cut(s) 978
MaeII ACGT 1 cut(s) 598
MaeIII GTNAC 4 cut(s) 452, 473, 604, 727
MalI GATC 7 cut(s) 152, 365, 424, 447, 659, 741, 816
MboI GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
MboII GAAGA 7 cut(s) 23, 212, 384, 496, 674, 895, 971
MhlI GDGCHC 3 cut(s) 132, 346, 392
MluCI AATT 9 cut(s) 78, 180, 577, 588, 679, 706, 809, 839, 846
MlyI GAGTC 4 cut(s) 245, 347, 425, 865
MmeI TCCRAC 2 cut(s) 261, 565
Mph1103I ATGCAT 1 cut(s) 169
MroI TCCGGA 2 cut(s) 91, 190
MseI TTAA 4 cut(s) 54, 300, 747, 1000
MslI CAYNNNNRTG 1 cut(s) 459
MspI CCGG 2 cut(s) 92, 191
MspR9I CCNGG 1 cut(s) 876
Mva1269I GAATGC 1 cut(s) 167
MvaI CCWGG 1 cut(s) 876
MwoI GCNNNNNNNGC 2 cut(s) 253, 387
NcoI CCATGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 460
NdeII GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
NlaIII CATG 4 cut(s) 86, 446, 771, 804
NlaIV GGNNCC 5 cut(s) 44, 130, 380, 527, 935
NmeAIII GCCGAG 1 cut(s) 335
NmuCI GTSAC 4 cut(s) 452, 473, 604, 727
NsiI ATGCAT 1 cut(s) 169
PagI TCATGA 1 cut(s) 82
PaqCI CACCTGC 1 cut(s) 983
PceI AGGCCT 1 cut(s) 493
PciSI GCTCTTC 1 cut(s) 397
PctI GAATGC 1 cut(s) 167
PfeI GAWTC 7 cut(s) 19, 95, 122, 293, 464, 698, 820
PkrI GCNGC 3 cut(s) 246, 969, 972
PleI GAGTC 4 cut(s) 244, 347, 425, 864
PpsI GAGTC 4 cut(s) 244, 347, 425, 864
Psp6I CCWGG 1 cut(s) 874
PspFI CCCAGC 2 cut(s) 920, 930
PspGI CCWGG 1 cut(s) 874
PspN4I GGNNCC 5 cut(s) 44, 130, 380, 527, 935
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 4 cut(s) 128, 129, 439, 934
PstI CTGCAG 1 cut(s) 473
RsaI GTAC 3 cut(s) 44, 527, 567
RsaNI GTAC 3 cut(s) 43, 526, 566
RseI CAYNNNNRTG 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 397
SaqAI TTAA 4 cut(s) 54, 300, 747, 1000
SatI GCNGC 3 cut(s) 245, 968, 971
Sau3AI GATC 7 cut(s) 150, 363, 422, 445, 657, 739, 814
Sau96I GGNCC 4 cut(s) 128, 129, 439, 934
SchI GAGTC 4 cut(s) 245, 347, 425, 865
ScrFI CCNGG 1 cut(s) 876
SduI GDGCHC 3 cut(s) 132, 346, 392
SfaNI GCATC 2 cut(s) 14, 154
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 439, 934
SmiMI CAYNNNNRTG 1 cut(s) 459
SmlI CTYRAG 3 cut(s) 275, 383, 536
SmoI CTYRAG 3 cut(s) 275, 383, 536
Sse9I AATT 9 cut(s) 78, 180, 577, 588, 679, 706, 809, 839, 846
SseBI AGGCCT 1 cut(s) 493
SspMI CTAG 1 cut(s) 978
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 1 cut(s) 874
StyI CCWWGG 2 cut(s) 307, 767
TaaI ACNGT 1 cut(s) 986
TaiI ACGT 1 cut(s) 601
TaqI TCGA 6 cut(s) 22, 117, 149, 296, 660, 823
TasI AATT 9 cut(s) 78, 180, 577, 588, 679, 706, 809, 839, 846
TfiI GAWTC 7 cut(s) 19, 95, 122, 293, 464, 698, 820
Tru1I TTAA 4 cut(s) 54, 300, 747, 1000
Tru9I TTAA 4 cut(s) 54, 300, 747, 1000
TscAI CASTG 2 cut(s) 406, 478
TseFI GTSAC 4 cut(s) 452, 473, 604, 727
TseI GCWGC 3 cut(s) 244, 967, 970
Tsp45I GTSAC 4 cut(s) 452, 473, 604, 727
TspDTI ATGAA 4 cut(s) 71, 99, 173, 972
TspGWI ACGGA 2 cut(s) 532, 545
TspRI CASTG 2 cut(s) 406, 478
VneI GTGCAC 1 cut(s) 342
VpaK11BI GGWCC 2 cut(s) 439, 934
XagI CCTNNNNNAGG 1 cut(s) 487
XapI RAATTY 4 cut(s) 78, 180, 588, 839
XspI CTAG 1 cut(s) 978
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.