MD17G1140200.v1.1

Mitochondrial PGP phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005815
Physical Location & Seq
Forward (+)
12598557 .. 12601402
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1140200.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGCAGTCGACCTCATCAGCTCTACTGCGCAAATGCTCTTACCCTCTCCCCCTTAACCACTTCCTCTTCCACTTCCACCATCGACACCCTCGCAAAACCCTCAACTTCCTTCCCCTCCCCCCAAAACCCCAAACCCACTCCTCAAACCACTGCGCGCTTTCCATCTCTCACACCAACAGTTGCAGCAGAGAGCACCAGGAGCAGAGCCAGAAGCACACCAACGGCGATTCCGACCCTTATCAGAACGATTTGTTGTTTCTGCAGTTGAAATCCTCCGGCGATACCGAGGCGGCCAGTAATCAAAGCCCAGAAGCCCCAAACGACGAACGAGGACGAGACCAAAGAGAGAGCGAAGCCGATAATTCCGAGGGACTTTTGACAAACATGTGGTGGGCAGACGTGAAAGCCGCACTGGGACAAAGGTTCAACTTTGGAGCCATAGTTCGTTCGGCCATGGTGCTCACAAAGGACAAGCACTTGGCGCTGCCGCATGTTTCTGTGCCTGATGTTAGGTACATTGATTGGCCTGAGTTGCATAGAAGGGGTTTCAAGGGTGTTGTGTTCGACAAGGATAATACTATCACTGCGCCGTATTCCTTGACGCTTTGGGGCCCTCTTGGGTCTTCGTTGGAGCAGTGTAAATCAGTTTTCGGACCCGATATCGCGGTGTTCAGTAACTCGGCTGGGCTTTATGAGTATGACCATGATGGTTCAAAAGCTAGGGAGCTTGAAGGGGCAATTGGAATTAAAGTCATAAGACATAGAGTGAAGAAACCAGCTGGAACATCTGAAGAGATCGAGAAGCATTTTGGTTGTAAATCTTCACACCTAATTATGGTGGGTGATCGGCCTTTCACTGATATTGTCTATGGAAATCAAAATGGCTTTCTCACTATTTTAACTGAACCGTTGAGTCTCAATGAGGAGCCGTTCATTGTTAGGCAGGTGAGGAAACTAGAAACATCCCTTGTGAATCATTGGTTTAAGAAAGGGTTAAAGCCCACCAGTCATAGGCTGTTGCCTGATGGCAAGCAGTGTGTCAAACATCCACCACCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00564 ko01100 map00564 map01100
Pfam Domains
Protein Families
PGP_phosphatase

Protein Analysis

354

Amino Acids

39.75

Weight (kDa)

8.44

Isoelectric Point (pI)

31.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGP_phosphatase PF09419 150 - 304 8.1e-43 Mitochondrial PGP phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 934
Acc16I TGCGCA 1 cut(s) 29
Acc36I ACCTGC 1 cut(s) 934
AccI GTMKAC 1 cut(s) 8
AccII CGCG 2 cut(s) 155, 665
AciI CCGC 4 cut(s) 290, 408, 488, 665
AcoI YGGCCR 2 cut(s) 291, 450
AcuI CTGAAG 1 cut(s) 810
AfaI GTAC 1 cut(s) 515
AfiI CCNNNNNNNGG 2 cut(s) 835, 1011
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 5 cut(s) 268, 427, 550, 714, 731
AjiI CACGTC 1 cut(s) 400
AjnI CCWGG 1 cut(s) 195
AjuI GAANNNNNNNTTGG 2 cut(s) 723, 755
AloI GAACNNNNNNTCC 2 cut(s) 426, 458
AluBI AGCT 4 cut(s) 20, 719, 727, 779
AluI AGCT 4 cut(s) 20, 719, 727, 779
Alw21I GWGCWC 2 cut(s) 195, 462
Alw26I GTCTC 2 cut(s) 330, 920
AoxI GGCC 5 cut(s) 291, 450, 524, 610, 848
ApaI GGGCCC 1 cut(s) 614
ApeKI GCWGC 2 cut(s) 183, 484
AspLEI GCGC 5 cut(s) 30, 155, 157, 484, 589
AspS9I GGNCC 3 cut(s) 610, 611, 653
AsuHPI GGTGA 2 cut(s) 854, 958
AvaII GGWCC 1 cut(s) 653
BaeGI GKGCMC 1 cut(s) 614
BanII GRGCYC 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 615
Bbv12I GWGCWC 2 cut(s) 195, 462
BbvI GCAGC 2 cut(s) 195, 471
BccI CCATC 4 cut(s) 87, 170, 701, 1019
BceAI ACGGC 3 cut(s) 238, 574, 913
BciT130I CCWGG 1 cut(s) 197
BcoDI GTCTC 2 cut(s) 330, 920
BfaI CTAG 2 cut(s) 720, 956
BfmI CTRYAG 1 cut(s) 260
BfoI RGCGCY 1 cut(s) 485
BfuAI ACCTGC 1 cut(s) 934
BisI GCNGC 5 cut(s) 184, 291, 408, 485, 488
BlsI GCNGC 5 cut(s) 185, 292, 409, 486, 489
Bme1390I CCNGG 1 cut(s) 197
Bme18I GGWCC 1 cut(s) 653
BmgBI CACGTC 1 cut(s) 400
BmgT120I GGNCC 3 cut(s) 610, 611, 653
BmiI GGNNCC 5 cut(s) 436, 611, 612, 655, 927
BmrFI CCNGG 1 cut(s) 197
BmrI ACTGGG 1 cut(s) 422
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 1 cut(s) 615
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 3 cut(s) 285, 366, 453
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 2 cut(s) 835, 1011
Bse1I ACTGG 3 cut(s) 294, 417, 1005
BseBI CCWGG 1 cut(s) 197
BseDI CCNNGG 3 cut(s) 285, 366, 453
BseGI GGATG 2 cut(s) 962, 1045
BseLI CCNNNNNNNGG 2 cut(s) 835, 1011
BseMII CTCAG 1 cut(s) 519
BseNI ACTGG 3 cut(s) 294, 417, 1005
BsePI GCGCGC 1 cut(s) 153
BseRI GAGGAG 2 cut(s) 130, 938
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 2 cut(s) 195, 471
BseYI CCCAGC 1 cut(s) 683
Bsh1236I CGCG 2 cut(s) 155, 665
BshFI GGCC 5 cut(s) 293, 452, 526, 612, 850
BsiHKAI GWGCWC 2 cut(s) 195, 462
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 2 cut(s) 384, 429
BslI CCNNNNNNNGG 2 cut(s) 835, 1011
BsmAI GTCTC 2 cut(s) 330, 920
BsmFI GGGAC 2 cut(s) 384, 429
BsnI GGCC 5 cut(s) 293, 452, 526, 612, 850
Bso31I GGTCTC 1 cut(s) 330
Bsp120I GGGCCC 1 cut(s) 610
Bsp1286I GDGCHC 3 cut(s) 195, 462, 614
Bsp143I GATC 2 cut(s) 795, 844
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 4 cut(s) 290, 408, 488, 665
BspANI GGCC 5 cut(s) 293, 452, 526, 612, 850
BspCNI CTCAG 1 cut(s) 520
BspFNI CGCG 2 cut(s) 155, 665
BspLI GGNNCC 5 cut(s) 436, 611, 612, 655, 927
BspMAI CTGCAG 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 934
BspTNI GGTCTC 1 cut(s) 330
BsrI ACTGG 3 cut(s) 294, 417, 1005
BssECI CCNNGG 3 cut(s) 285, 366, 453
BssHII GCGCGC 1 cut(s) 153
BssMI GATC 2 cut(s) 795, 844
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 197
Bst4CI ACNGT 2 cut(s) 179, 909
Bst6I CTCTTC 2 cut(s) 71, 786
BstC8I GCNNGC 2 cut(s) 155, 1031
BstDEI CTNAG 1 cut(s) 528
BstDSI CCRYGG 1 cut(s) 453
BstF5I GGATG 2 cut(s) 962, 1045
BstFNI CGCG 2 cut(s) 155, 665
BstH2I RGCGCY 1 cut(s) 485
BstHHI GCGC 5 cut(s) 30, 155, 157, 484, 589
BstKTI GATC 2 cut(s) 798, 847
BstMAI GTCTC 2 cut(s) 330, 920
BstMBI GATC 2 cut(s) 795, 844
BstMWI GCNNNNNNNGC 3 cut(s) 199, 481, 532
BstNI CCWGG 1 cut(s) 197
BstNSI RCATGY 2 cut(s) 388, 494
BstSCI CCNGG 1 cut(s) 195
BstSFI CTRYAG 1 cut(s) 260
BstSLI GKGCMC 1 cut(s) 614
BstUI CGCG 2 cut(s) 155, 665
BstV1I GCAGC 2 cut(s) 195, 471
BstV2I GAAGAC 1 cut(s) 615
BsuRI GGCC 5 cut(s) 293, 452, 526, 612, 850
BtgI CCRYGG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 400
BtsCI GGATG 2 cut(s) 962, 1045
BtsI GCAGTG 4 cut(s) 148, 582, 641, 1040
BtsIMutI CAGTG 6 cut(s) 148, 410, 582, 641, 855, 1040
BveI ACCTGC 1 cut(s) 934
Cac8I GCNNGC 2 cut(s) 155, 1031
CfoI GCGC 5 cut(s) 30, 155, 157, 484, 589
Cfr13I GGNCC 3 cut(s) 610, 611, 653
CseI GACGC 1 cut(s) 610
Csp6I GTAC 1 cut(s) 514
CviAII CATG 4 cut(s) 385, 454, 491, 704
CviQI GTAC 1 cut(s) 514
DdeI CTNAG 1 cut(s) 528
DpnI GATC 2 cut(s) 797, 846
DpnII GATC 2 cut(s) 795, 844
EaeI YGGCCR 2 cut(s) 291, 450
Eam1104I CTCTTC 2 cut(s) 71, 786
EarI CTCTTC 2 cut(s) 71, 786
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 614
Eco31I GGTCTC 1 cut(s) 330
Eco32I GATATC 1 cut(s) 661
Eco47I GGWCC 1 cut(s) 653
Eco57I CTGAAG 1 cut(s) 810
EcoO109I RGGNCCY 2 cut(s) 610, 611
EcoRII CCWGG 1 cut(s) 195
EcoRV GATATC 1 cut(s) 661
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 614
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 4 cut(s) 388, 457, 494, 707
FaqI GGGAC 2 cut(s) 384, 429
FatI CATG 4 cut(s) 384, 453, 490, 703
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 5 cut(s) 184, 291, 408, 485, 488
FokI GGATG 2 cut(s) 949, 1032
FriOI GRGCYC 1 cut(s) 614
Fsp4HI GCNGC 5 cut(s) 184, 291, 408, 485, 488
FspBI CTAG 2 cut(s) 720, 956
FspI TGCGCA 1 cut(s) 29
GlaI GCGC 5 cut(s) 29, 154, 156, 483, 588
GluI GCNGC 5 cut(s) 184, 291, 408, 485, 488
GsaI CCCAGC 1 cut(s) 687
HaeII RGCGCY 1 cut(s) 485
HaeIII GGCC 5 cut(s) 293, 452, 526, 612, 850
HapII CCGG 1 cut(s) 276
HgaI GACGC 1 cut(s) 610
HhaI GCGC 5 cut(s) 30, 155, 157, 484, 589
Hin1II CATG 4 cut(s) 388, 457, 494, 707
Hin6I GCGC 5 cut(s) 28, 153, 155, 482, 587
HinP1I GCGC 5 cut(s) 28, 153, 155, 482, 587
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 3 cut(s) 227, 913, 973
HpaII CCGG 1 cut(s) 276
HphI GGTGA 2 cut(s) 854, 958
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 5 cut(s) 232, 243, 367, 653, 790
Hpy188III TCNNGA 1 cut(s) 799
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 3 cut(s) 119, 534, 725
HpyCH4III ACNGT 2 cut(s) 179, 909
HpyCH4IV ACGT 1 cut(s) 399
HpyCH4V TGCA 4 cut(s) 4, 183, 262, 535
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 481, 532
HpyF3I CTNAG 1 cut(s) 528
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 4 cut(s) 388, 457, 494, 707
HspAI GCGC 5 cut(s) 28, 153, 155, 482, 587
Kzo9I GATC 2 cut(s) 795, 844
LmnI GCTCC 5 cut(s) 199, 434, 631, 724, 925
Lsp1109I GCAGC 2 cut(s) 195, 471
MaeI CTAG 2 cut(s) 720, 956
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 1 cut(s) 674
MalI GATC 2 cut(s) 797, 846
MboI GATC 2 cut(s) 795, 844
MboII GAAGA 5 cut(s) 58, 615, 781, 803, 813
MfeI CAATTG 1 cut(s) 738
MhlI GDGCHC 3 cut(s) 195, 462, 614
MluCI AATT 4 cut(s) 361, 738, 744, 831
MlyI GAGTC 1 cut(s) 922
MmeI TCCRAC 2 cut(s) 255, 609
MseI TTAA 5 cut(s) 54, 747, 899, 984, 995
MspA1I CMGCKG 1 cut(s) 779
MspI CCGG 1 cut(s) 276
MspR9I CCNGG 1 cut(s) 197
MunI CAATTG 1 cut(s) 738
MvaI CCWGG 1 cut(s) 197
MvnI CGCG 2 cut(s) 155, 665
MwoI GCNNNNNNNGC 3 cut(s) 199, 481, 532
NcoI CCATGG 1 cut(s) 453
NdeII GATC 2 cut(s) 795, 844
NlaIII CATG 4 cut(s) 388, 457, 494, 707
NlaIV GGNNCC 5 cut(s) 436, 611, 612, 655, 927
NmeAIII GCCGAG 1 cut(s) 659
NsbI TGCGCA 1 cut(s) 29
NspI RCATGY 2 cut(s) 388, 494
PaqCI CACCTGC 1 cut(s) 934
PauI GCGCGC 1 cut(s) 153
PciI ACATGT 1 cut(s) 384
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 2 cut(s) 227, 973
PkrI GCNGC 5 cut(s) 185, 292, 409, 486, 489
PleI GAGTC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 921
PscI ACATGT 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 195
PspFI CCCAGC 1 cut(s) 683
PspGI CCWGG 1 cut(s) 195
PspN4I GGNNCC 5 cut(s) 436, 611, 612, 655, 927
PspOMI GGGCCC 1 cut(s) 610
PspPI GGNCC 3 cut(s) 610, 611, 653
PstI CTGCAG 1 cut(s) 264
PteI GCGCGC 1 cut(s) 153
PvuII CAGCTG 1 cut(s) 779
RsaI GTAC 1 cut(s) 515
RsaNI GTAC 1 cut(s) 514
SalI GTCGAC 1 cut(s) 7
SaqAI TTAA 5 cut(s) 54, 747, 899, 984, 995
SatI GCNGC 5 cut(s) 184, 291, 408, 485, 488
Sau3AI GATC 2 cut(s) 795, 844
Sau96I GGNCC 3 cut(s) 610, 611, 653
SchI GAGTC 1 cut(s) 922
ScrFI CCNGG 1 cut(s) 197
SduI GDGCHC 3 cut(s) 195, 462, 614
SfcI CTRYAG 1 cut(s) 260
SinI GGWCC 1 cut(s) 653
Sse9I AATT 4 cut(s) 361, 738, 744, 831
SsiI CCGC 4 cut(s) 290, 408, 488, 665
SspMI CTAG 2 cut(s) 720, 956
StyD4I CCNGG 1 cut(s) 195
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 179, 909
TaiI ACGT 1 cut(s) 402
TaqI TCGA 4 cut(s) 8, 82, 564, 798
TasI AATT 4 cut(s) 361, 738, 744, 831
TauI GCSGC 3 cut(s) 293, 410, 490
TfiI GAWTC 2 cut(s) 227, 973
Tru1I TTAA 5 cut(s) 54, 747, 899, 984, 995
Tru9I TTAA 5 cut(s) 54, 747, 899, 984, 995
TscAI CASTG 6 cut(s) 155, 417, 589, 641, 862, 1040
TseI GCWGC 2 cut(s) 183, 484
TspDTI ATGAA 1 cut(s) 922
TspRI CASTG 6 cut(s) 155, 417, 589, 641, 862, 1040
VpaK11BI GGWCC 1 cut(s) 653
XceI RCATGY 2 cut(s) 388, 494
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 2 cut(s) 720, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.