MD17G1250300.v1.1
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004524
Physical Location & Seq
Forward (+)
29985475 .. 29987990
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1250300.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGGTTTTGCAAGTTCTTTGCTTGGCTTTCCTTGTATGCAACTCTCTCTCACTTGTCAGTTGTATAAATGAAGAAGGATTGGCTCTACTTTCGTTCAAGGAATCCTTAACGAAAAACCCCGAAAGCTCTCTGAGTACCTGGAACTCATCAGACCAAACCCCATGTTCATGGGCTGGAGTTACATGCAGAGAAGAAAAGGTTGTATCTCTCAGTATTCCAAACAAACAGCTTTCTGGGATTTTCCCTCGTGCTCTTGGGAACCTCTCCGCCCTGCGCCATCTGAATCTCCGGGAGAACAAACTCTCCGGAAGCTTGCCGTCTGAGCTTTTCAATGCAAAAGAGCTCCAGAGCTTGGTGCTGTCTGGAAATTCATTATCTGGTTCTGTTCCAGCTGAGATAGGGAAGCTCAGTTACCTGCAAGCATTAGATCTTTCGCAAAATTCATTCAATGGTTCGATACCTTCATCTATAGTTCAGTGCAAGAGACTAAAGATCCTTGTCCTTGCTCAAAACCATTTCTCTGGCTCCCTTCCACAAGGTATGGGAATCAGTTTTGTTCATCTTCAAAAGCTCAATATTTCCTTCAATATTCTCGGCGGTTCGATTCCTGAGGAAATGAGCAACTTGTCAAGTTTGAGATCAACCCTTGATATGTCTCATAACTTGTTCAACGGTTCTATTCCGCCATCCCTAGGAAGCTTGCCTGAGACGGTATACATTGATCTCAGTTACAACAATCTCAGTGGACCGATTCCACTGAATGGTGCTCTGATCAATATAGGACCAACGGCTTTCGTAGGCAATCGTTTACTCTGTGGACTGCCGTTGAAGATTTCATGCTCTTCAAGCAGTCAAAACTTCGATTCTCAAAATTCAGGCGGCAGCTCTGGTATGAATGGGAAACACAACTCAAGCAGAGGTATTTTTGTGATTACTGTTTCTGCAGGCGTCATCGCTGGAATTTTTATTGCTGGGTTTTTGTTCTCCAACTGGTACAAGAAGGTTTGTACCTGCAAAATACGTGAACGTTTTGCTGGCTGCAAATTTGAACAAAAACTAAAGGTCGGAAATGACCTATTCTGCTTCAAAAGACATGGCATGGATACCGTACCAGAGAATTTGGAGCATTACTATTTTGTGCAAGTGGATCTGCAGGTGAATTTAGATCTTGACAAACTGCTGAAATCATCTGCTTTTCTTCTGGGTAATAGTGGGATCGGGATTGTGTACAGAGTTGTTCTTGAAGACGGAAGAACTGTAGCAGTGAGAAGATTGGGAGATGGAGGTTCCCAAAGGTTCAAGGTATTCCAAAATCAAATTGAAGCAATAGGAAAGATGAGGCATCCAAATATTGTGAATCTTTTAGCATATTGCTGGTCTGTTGATGAGAAACTGCTTATCTATGATTATATCCCAAATGGTGACCTTGCTTCTGCAATTCATGGGAAACCTGGAATGGTATCCTTCACACCTCTTTCTTGGCCTGCACGTCTGAAAATCATGAATGGCTTGGCAAAAGGATTGGCTTATATTCATGAATTTAGTCCCAGAAGATATGTGCATGGAAACTTGAGGCCTAGAAACATACTGCTTGGACAAAACATGGAACCCCGTATCTCCGACTTCGGACTAGGCCGCCTTGCTAACTTAACTGAAGAGTCCTTGTCATCATCGTCATTTCAACTGGAACAAATAATGGCTGAAACACCACCTCAGAATTCTCCATATGTGCAAAGGACATCGAGCTCACTGGCAGCACCCGGACCTTGCTATAAAGCTCCTGAAGCATCAAAAGTAACCAAGCCATCACAGAAATGGGATGTTTACTCATTTGGGGTAGTTATTCTGGAGATGATTTCTGCCAAAATGCCATTTATGAGAATTGGTTCTTTGGAAATGGATTTGATACAACAGTTTCAGCTCTGCATTGAGGAAAGGAAGCCACTCTGCGATCTGTTAGACCCGTTCCTAGCTCCTGATGTAGACATGAAAGAGGAGATTGTTGCGGTGCTGAAAATCGCTCTTGCTTGTGTTCATAAAAGCCCTGAAAAGAGACCTTCTATGAGGTTTGTCTGTGATAATTTGGCCAGGTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

700

Amino Acids

76.64

Weight (kDa)

8.74

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 62 1.7e-14 Leucine rich repeat N-terminal domain
LRR_8 PF13855 67 - 125 3.7e-08 Leucine rich repeat
LRR_14 PF23598 69 - 214 1e-11 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 401 - 554 6.9e-21 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 401 - 692 3.1e-26 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1144
Acc36I ACCTGC 3 cut(s) 423, 1019, 1144
AccB7I CCANNNNNTGG 2 cut(s) 352, 761
AccI GTMKAC 2 cut(s) 714, 1983
AccIII TCCGGA 1 cut(s) 305
AciI CCGC 6 cut(s) 267, 597, 683, 879, 1636, 2006
AclI AACGTT 1 cut(s) 1027
AclWI GGATC 3 cut(s) 487, 1155, 1223
AcoI YGGCCR 1 cut(s) 2085
AcsI RAATTY 9 cut(s) 367, 439, 871, 960, 1043, 1117, 1159, 1538, 1717
AcuI CTGAAG 2 cut(s) 1674, 1803
AcyI GRCGYC 1 cut(s) 948
AfaI GTAC 5 cut(s) 136, 995, 1009, 1110, 1229
AfiI CCNNNNNNNGG 3 cut(s) 352, 692, 761
AjiI CACGTC 1 cut(s) 1490
AjnI CCWGG 3 cut(s) 137, 1450, 2087
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
Alw21I GWGCWC 4 cut(s) 253, 345, 769, 1748
Alw26I GTCTC 4 cut(s) 478, 660, 701, 2047
AlwI GGATC 3 cut(s) 487, 1155, 1223
Aor13HI TCCGGA 1 cut(s) 305
AoxI GGCC 5 cut(s) 1481, 1574, 1633, 2085, 2094
ApeKI GCWGC 3 cut(s) 882, 1038, 1754
ApoI RAATTY 9 cut(s) 367, 439, 871, 960, 1043, 1117, 1159, 1538, 1717
Asp700I GAANNNNTTC 1 cut(s) 1885
AspA2I CCTAGG 1 cut(s) 691
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 3 cut(s) 746, 782, 1763
AsuC2I CCSGG 2 cut(s) 290, 1761
AsuHPI GGTGA 2 cut(s) 1168, 1433
AvaII GGWCC 3 cut(s) 746, 782, 1763
AvrII CCTAGG 1 cut(s) 691
AxyI CCTNAGG 1 cut(s) 609
BaeI ACNNNNGTAYC 2 cut(s) 1898, 1931
BalI TGGCCA 1 cut(s) 2087
BanII GRGCYC 2 cut(s) 345, 1748
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 1251
Bbv12I GWGCWC 4 cut(s) 253, 345, 769, 1748
BbvI GCAGC 3 cut(s) 894, 1025, 1766
BccI CCATC 4 cut(s) 285, 694, 1274, 1813
BceAI ACGGC 3 cut(s) 301, 804, 808
BciT130I CCWGG 3 cut(s) 139, 1452, 2089
BciVI GTATCC 2 cut(s) 1096, 1471
BclI TGATCA 1 cut(s) 771
BcnI CCSGG 2 cut(s) 290, 1761
BcoDI GTCTC 4 cut(s) 478, 660, 701, 2047
BfaI CTAG 5 cut(s) 692, 1578, 1631, 1970, 2098
BfmI CTRYAG 4 cut(s) 468, 942, 1151, 1257
BfuAI ACCTGC 3 cut(s) 423, 1019, 1144
BfuI GTATCC 2 cut(s) 1096, 1471
BglI GCCNNNNNGGC 1 cut(s) 2093
BglII AGATCT 2 cut(s) 427, 1165
BisI GCNGC 5 cut(s) 880, 883, 1039, 1636, 1755
BlnI CCTAGG 1 cut(s) 691
BlsI GCNGC 5 cut(s) 881, 884, 1040, 1637, 1756
Bme1390I CCNGG 5 cut(s) 139, 290, 1452, 1761, 2089
Bme18I GGWCC 3 cut(s) 746, 782, 1763
BmgBI CACGTC 1 cut(s) 1490
BmgT120I GGNCC 3 cut(s) 746, 782, 1763
BmiI GGNNCC 4 cut(s) 260, 526, 1288, 1608
BmrFI CCNGG 5 cut(s) 139, 290, 1452, 1761, 2089
BmsI GCATC 2 cut(s) 1351, 1796
BpiI GAAGAC 1 cut(s) 1251
BpmI CTGGAG 3 cut(s) 195, 329, 1868
BpuEI CTTGAG 2 cut(s) 895, 1591
BpuMI CCSGG 2 cut(s) 290, 1761
BsaAI YACGTR 1 cut(s) 1022
BsaHI GRCGYC 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 2047
BsaJI CCNNGG 1 cut(s) 691
BsaWI WCCGGW 1 cut(s) 305
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 3 cut(s) 352, 692, 761
Bse1I ACTGG 3 cut(s) 995, 1689, 1755
Bse21I CCTNAGG 1 cut(s) 609
BseAI TCCGGA 1 cut(s) 305
BseBI CCWGG 3 cut(s) 139, 1452, 2089
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 3 cut(s) 686, 1342, 1825
BseLI CCNNNNNNNGG 3 cut(s) 352, 692, 761
BseNI ACTGG 3 cut(s) 995, 1689, 1755
BseRI GAGGAG 1 cut(s) 2009
BseXI GCAGC 3 cut(s) 894, 1025, 1766
BseYI CCCAGC 1 cut(s) 971
BsgI GTGCAG 1 cut(s) 1470
BshFI GGCC 5 cut(s) 1483, 1576, 1635, 2087, 2096
BsiHKAI GWGCWC 4 cut(s) 253, 345, 769, 1748
BsiSI CCGG 3 cut(s) 289, 306, 1761
BslFI GGGAC 1 cut(s) 1530
BslI CCNNNNNNNGG 3 cut(s) 352, 692, 761
BsmAI GTCTC 4 cut(s) 478, 660, 701, 2047
BsmBI CGTCTC 1 cut(s) 701
BsmFI GGGAC 1 cut(s) 1530
BsnI GGCC 5 cut(s) 1483, 1576, 1635, 2087, 2096
Bso31I GGTCTC 1 cut(s) 2047
Bsp1286I GDGCHC 4 cut(s) 253, 345, 769, 1748
Bsp13I TCCGGA 1 cut(s) 305
Bsp1407I TGTACA 1 cut(s) 1227
Bsp143I GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
BspACI CCGC 6 cut(s) 267, 597, 683, 879, 1636, 2006
BspANI GGCC 5 cut(s) 1483, 1576, 1635, 2087, 2096
BspEI TCCGGA 1 cut(s) 305
BspHI TCATGA 2 cut(s) 1500, 1534
BspLI GGNNCC 4 cut(s) 260, 526, 1288, 1608
BspMAI CTGCAG 2 cut(s) 946, 1155
BspMI ACCTGC 3 cut(s) 423, 1019, 1144
BspPI GGATC 3 cut(s) 487, 1155, 1223
BspQI GCTCTTC 1 cut(s) 847
BspTNI GGTCTC 1 cut(s) 2047
BsrGI TGTACA 1 cut(s) 1227
BsrI ACTGG 3 cut(s) 995, 1689, 1755
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
BssNAI GTATAC 1 cut(s) 715
BssNI GRCGYC 1 cut(s) 948
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 691
Bst1107I GTATAC 1 cut(s) 715
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 139, 1452, 2089
Bst4CI ACNGT 6 cut(s) 674, 712, 937, 1108, 1258, 1914
Bst6I CTCTTC 2 cut(s) 847, 1650
BstACI GRCGYC 1 cut(s) 948
BstAUI TGTACA 1 cut(s) 1227
BstBAI YACGTR 1 cut(s) 1022
BstC8I GCNNGC 6 cut(s) 314, 420, 701, 946, 1036, 1485
BstEII GGTNACC 1 cut(s) 1421
BstF5I GGATG 3 cut(s) 686, 1342, 1825
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 9 cut(s) 430, 495, 641, 724, 774, 1150, 1168, 1218, 1954
BstMAI GTCTC 4 cut(s) 478, 660, 701, 2047
BstMBI GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
BstMWI GCNNNNNNNGC 4 cut(s) 322, 846, 1784, 2093
BstNI CCWGG 3 cut(s) 139, 1452, 2089
BstNSI RCATGY 1 cut(s) 186
BstPI GGTNACC 1 cut(s) 1421
BstSCI CCNGG 5 cut(s) 137, 288, 1450, 1759, 2087
BstSFI CTRYAG 4 cut(s) 468, 942, 1151, 1257
BstV1I GCAGC 3 cut(s) 894, 1025, 1766
BstV2I GAAGAC 1 cut(s) 1251
BstX2I RGATCY 4 cut(s) 427, 492, 1147, 1165
BstXI CCANNNNNNTGG 2 cut(s) 168, 521
BstYI RGATCY 4 cut(s) 427, 492, 1147, 1165
BstZ17I GTATAC 1 cut(s) 715
Bsu36I CCTNAGG 1 cut(s) 609
BsuI GTATCC 2 cut(s) 1096, 1471
BsuRI GGCC 5 cut(s) 1483, 1576, 1635, 2087, 2096
BtgZI GCGATG 1 cut(s) 937
BtrI CACGTC 1 cut(s) 1490
BtsCI GGATG 3 cut(s) 686, 1342, 1825
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 5 cut(s) 482, 748, 755, 1269, 1748
BveI ACCTGC 3 cut(s) 423, 1019, 1144
Cac8I GCNNGC 6 cut(s) 314, 420, 701, 946, 1036, 1485
CciI TCATGA 2 cut(s) 1500, 1534
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 3 cut(s) 746, 782, 1763
CseI GACGC 1 cut(s) 937
Csp6I GTAC 5 cut(s) 135, 994, 1008, 1109, 1228
CspCI CAANNNNNGTGG 2 cut(s) 724, 759
CviQI GTAC 5 cut(s) 135, 994, 1008, 1109, 1228
DpnI GATC 9 cut(s) 429, 494, 640, 723, 773, 1149, 1167, 1217, 1953
DpnII GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
EaeI YGGCCR 1 cut(s) 2085
Eam1104I CTCTTC 2 cut(s) 847, 1650
EarI CTCTTC 2 cut(s) 847, 1650
EciI GGCGGA 2 cut(s) 256, 672
Ecl136II GAGCTC 2 cut(s) 343, 1746
Eco130I CCWWGG 1 cut(s) 691
Eco147I AGGCCT 1 cut(s) 1576
Eco24I GRGCYC 2 cut(s) 345, 1748
Eco31I GGTCTC 1 cut(s) 2047
Eco47I GGWCC 3 cut(s) 746, 782, 1763
Eco53kI GAGCTC 2 cut(s) 343, 1746
Eco57I CTGAAG 2 cut(s) 1674, 1803
Eco81I CCTNAGG 1 cut(s) 609
Eco91I GGTNACC 1 cut(s) 1421
EcoICRI GAGCTC 2 cut(s) 343, 1746
EcoO65I GGTNACC 1 cut(s) 1421
EcoRI GAATTC 1 cut(s) 1717
EcoRII CCWGG 3 cut(s) 137, 1450, 2087
EcoT14I CCWWGG 1 cut(s) 691
EcoT38I GRGCYC 2 cut(s) 345, 1748
ErhI CCWWGG 1 cut(s) 691
Esp3I CGTCTC 1 cut(s) 701
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 1530
FauNDI CATATG 1 cut(s) 1726
FbaI TGATCA 1 cut(s) 771
FblI GTMKAC 2 cut(s) 714, 1983
Fnu4HI GCNGC 5 cut(s) 880, 883, 1039, 1636, 1755
FokI GGATG 3 cut(s) 673, 1329, 1832
FriOI GRGCYC 2 cut(s) 345, 1748
Fsp4HI GCNGC 5 cut(s) 880, 883, 1039, 1636, 1755
FspBI CTAG 5 cut(s) 692, 1578, 1631, 1970, 2098
GlaI GCGC 1 cut(s) 275
GluI GCNGC 5 cut(s) 880, 883, 1039, 1636, 1755
GsaI CCCAGC 1 cut(s) 975
GsuI CTGGAG 3 cut(s) 195, 329, 1868
HaeIII GGCC 5 cut(s) 1483, 1576, 1635, 2087, 2096
HapII CCGG 3 cut(s) 289, 306, 1761
HgaI GACGC 1 cut(s) 937
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 948
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HindIII AAGCTT 2 cut(s) 310, 697
HinfI GANTC 8 cut(s) 101, 283, 546, 604, 751, 863, 1357, 1658
HpaII CCGG 3 cut(s) 289, 306, 1761
HphI GGTGA 2 cut(s) 1168, 1433
Hpy166II GTNNAC 8 cut(s) 715, 746, 809, 818, 1025, 1228, 1825, 1984
Hpy8I GTNNAC 8 cut(s) 715, 746, 809, 818, 1025, 1228, 1825, 1984
HpyAV CCTTC 7 cut(s) 68, 471, 539, 592, 994, 1474, 2067
HpyCH4III ACNGT 6 cut(s) 674, 712, 937, 1108, 1258, 1914
HpyCH4IV ACGT 3 cut(s) 1021, 1027, 1489
HpyF10VI GCNNNNNNNGC 4 cut(s) 322, 846, 1784, 2093
HpySE526I ACGT 3 cut(s) 1021, 1027, 1489
Hsp92I GRCGYC 1 cut(s) 948
HspAI GCGC 1 cut(s) 274
Kpn2I TCCGGA 1 cut(s) 305
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 5 cut(s) 348, 530, 1123, 1783, 1978
Lsp1109I GCAGC 3 cut(s) 894, 1025, 1766
LweI GCATC 2 cut(s) 1351, 1796
MaeI CTAG 5 cut(s) 692, 1578, 1631, 1970, 2098
MaeII ACGT 3 cut(s) 1021, 1027, 1489
MaeIII GTNAC 5 cut(s) 178, 410, 728, 1421, 1795
MalI GATC 9 cut(s) 429, 494, 640, 723, 773, 1149, 1167, 1217, 1953
MboI GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
MflI RGATCY 4 cut(s) 427, 492, 1147, 1165
MhlI GDGCHC 4 cut(s) 253, 345, 769, 1748
MlsI TGGCCA 1 cut(s) 2087
MluNI TGGCCA 1 cut(s) 2087
MlyI GAGTC 1 cut(s) 1667
MmeI TCCRAC 3 cut(s) 1011, 1045, 1644
Mox20I TGGCCA 1 cut(s) 2087
MroI TCCGGA 1 cut(s) 305
MroXI GAANNNNTTC 1 cut(s) 1885
MscI TGGCCA 1 cut(s) 2087
MseI TTAA 2 cut(s) 107, 1649
MslI CAYNNNNRTG 2 cut(s) 166, 1813
Msp20I TGGCCA 1 cut(s) 2087
MspA1I CMGCKG 1 cut(s) 392
MspI CCGG 3 cut(s) 289, 306, 1761
MspR9I CCNGG 5 cut(s) 139, 290, 1452, 1761, 2089
MvaI CCWGG 3 cut(s) 139, 1452, 2089
MwoI GCNNNNNNNGC 4 cut(s) 322, 846, 1784, 2093
NciI CCSGG 2 cut(s) 290, 1761
NdeI CATATG 1 cut(s) 1726
NdeII GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
NlaIV GGNNCC 4 cut(s) 260, 526, 1288, 1608
NmeAIII GCCGAG 1 cut(s) 573
NmuCI GTSAC 1 cut(s) 1421
NspI RCATGY 1 cut(s) 186
PagI TCATGA 2 cut(s) 1500, 1534
PaqCI CACCTGC 1 cut(s) 1144
PceI AGGCCT 1 cut(s) 1576
PciSI GCTCTTC 1 cut(s) 847
PdmI GAANNNNTTC 1 cut(s) 1885
PfeI GAWTC 7 cut(s) 101, 283, 546, 604, 751, 863, 1357
PflMI CCANNNNNTGG 2 cut(s) 352, 761
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 5 cut(s) 881, 884, 1040, 1637, 1756
PleI GAGTC 1 cut(s) 1666
PpsI GAGTC 1 cut(s) 1666
Ppu21I YACGTR 1 cut(s) 1022
Psp124BI GAGCTC 2 cut(s) 345, 1748
Psp1406I AACGTT 1 cut(s) 1027
Psp6I CCWGG 3 cut(s) 137, 1450, 2087
PspEI GGTNACC 1 cut(s) 1421
PspFI CCCAGC 1 cut(s) 971
PspGI CCWGG 3 cut(s) 137, 1450, 2087
PspN4I GGNNCC 4 cut(s) 260, 526, 1288, 1608
PspPI GGNCC 3 cut(s) 746, 782, 1763
PstI CTGCAG 2 cut(s) 946, 1155
PsuI RGATCY 4 cut(s) 427, 492, 1147, 1165
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 5 cut(s) 136, 995, 1009, 1110, 1229
RsaNI GTAC 5 cut(s) 135, 994, 1008, 1109, 1228
RseI CAYNNNNRTG 2 cut(s) 166, 1813
SacI GAGCTC 2 cut(s) 345, 1748
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 2 cut(s) 107, 1649
SatI GCNGC 5 cut(s) 880, 883, 1039, 1636, 1755
Sau3AI GATC 9 cut(s) 427, 492, 638, 721, 771, 1147, 1165, 1215, 1951
Sau96I GGNCC 3 cut(s) 746, 782, 1763
SchI GAGTC 1 cut(s) 1667
ScrFI CCNGG 5 cut(s) 139, 290, 1452, 1761, 2089
SduI GDGCHC 4 cut(s) 253, 345, 769, 1748
SfaNI GCATC 2 cut(s) 1351, 1796
SfcI CTRYAG 4 cut(s) 468, 942, 1151, 1257
SfiI GGCCNNNNNGGCC 1 cut(s) 2093
SinI GGWCC 3 cut(s) 746, 782, 1763
SmiMI CAYNNNNRTG 2 cut(s) 166, 1813
SmlI CTYRAG 2 cut(s) 910, 1570
SmoI CTYRAG 2 cut(s) 910, 1570
SseBI AGGCCT 1 cut(s) 1576
SsiI CCGC 6 cut(s) 267, 597, 683, 879, 1636, 2006
SspI AATATT 3 cut(s) 577, 589, 1351
SspMI CTAG 5 cut(s) 692, 1578, 1631, 1970, 2098
SstI GAGCTC 2 cut(s) 345, 1748
StuI AGGCCT 1 cut(s) 1576
StyD4I CCNGG 5 cut(s) 137, 288, 1450, 1759, 2087
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 6 cut(s) 674, 712, 937, 1108, 1258, 1914
TaiI ACGT 3 cut(s) 1024, 1030, 1492
TaqI TCGA 4 cut(s) 455, 602, 861, 1742
TaqII GACCGA 1 cut(s) 763
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 2 cut(s) 882, 1638
TfiI GAWTC 7 cut(s) 101, 283, 546, 604, 751, 863, 1357
Tru1I TTAA 2 cut(s) 107, 1649
Tru9I TTAA 2 cut(s) 107, 1649
TscAI CASTG 5 cut(s) 482, 748, 762, 1269, 1755
TseFI GTSAC 1 cut(s) 1421
TseI GCWGC 3 cut(s) 882, 1038, 1754
Tsp45I GTSAC 1 cut(s) 1421
TspGWI ACGGA 1 cut(s) 1263
TspRI CASTG 5 cut(s) 482, 748, 762, 1269, 1755
Van91I CCANNNNNTGG 2 cut(s) 352, 761
VpaK11BI GGWCC 3 cut(s) 746, 782, 1763
XapI RAATTY 9 cut(s) 367, 439, 871, 960, 1043, 1117, 1159, 1538, 1717
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1812
XmaJI CCTAGG 1 cut(s) 691
XmiI GTMKAC 2 cut(s) 714, 1983
XmnI GAANNNNTTC 1 cut(s) 1885
XspI CTAG 5 cut(s) 692, 1578, 1631, 1970, 2098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.