pycom17g25420
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0004524
Physical Location & Seq
Forward (+)
23505395 .. 23507811
2417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g25420.1

Sequence Viewer

Length: 2103 bp
ATGGTTTTGCAAGTTCTTTGCTTGGCTTTCCTTGTATGCAACTCTCTCTCACTTGTCAGTTGTATAAATGAAGAAGGATTGGCTCTACTTTCGTTCAAGGAATCCTTAACGAAAAACGCCGAAAGCTCTCTGAGTACCTGGAACTCATCAGACCAAACCCCATGTTCATGGGTTGGAGTTACATGCAGAGAAGAAAAGGTTGTATCTCTCAGCATTCCAAACATACAGCTTTCTGGGATTTTCCCTCGTGCTCTTGGGAACCTCTCCGCCCTGCGCCATCTGAATCTTCGGGAGAACAAACTCTCCGGAAGCTTGCCGTCTGAGCTTTTCAATGCAAAAGAGCTCCAGAGCTTGGTGCTGTCCGGAAATTCATTATCTGGTTCTGTTCCAGCTGAGATAGGGAAGCTCAGTTACCTGCAAACATTAGATCTTTCGCAAAATTCATTCAATGGTTCGATACCTTCATCTATAGTTCAGTGCAAGAGACTAAAGATCCTTGTCCTTGCTCAAAACCATTTCTCTGGCTCCCTTCCACAAGGTATGGGAATCAGTTTTGTTCATCTTGAAAAGCTCAATATTTCCTTCAATATTCTCAGCGGTTCGATTCCTGAGGAAATGAGCAACTTGTCAAGTTTGAGATCAACCCTTGATATGTCTCATAACTTGTTCAACGGTTCTATTCCGCCATCCCTAGGAAGCTTGCCTGAGACAGTATACATTGATCTCAGTTACAACAATCTCAGTGGACCGATTCCACTGAATGGTGCTCTGATCAATATAGGACCAACGGCTTTCGTAGGCAATCGTTTACTCTGTGGACTGCCGTTGAAGATTTCATGCTCTTCAAACAGTCAAAGCTTAAATTCTCAAAATTCAGGTGGCAGCTCTGGTACGAATGGGAAACACAACAACTCAAGCAGAGGTATTTTTGTGATTACTGTTGCTGCAGGCGTCATCGCTGGAATTTTTATTGCTGGGTTTTTGTTCTCCAACTGGTACAAGAAGGTTTGTACCTGCAAAATACGTGAACATTTTGCTGGCTGCAAATTTGAACAAAAATTGAAGGTCGGAAATGACCTATTCTGCTTCAAAAGACATGGCATGGATACCGTACCAGAGAATTTGGAGCATTACCATTTTGTGCAAGTGGATCTGCAGGTGAATTTAGATCTTGACAAACTGCTGAAATCATCTGCTTTTCTTCTGGGTAATAGTGGGATCGGGATTGTGTACAGAGTTGTTCTTGAAGACGGAAGAACTTTAGCAGTGAGAAGTTTGGGAGATGGAGGTTCCCAAAGGTTCAAGGTATTCCAAAATCAAATTGAAGCAATAGGAAAGATGAGGCATCCAAATATTGTGAATCTTTTAGCATATTGCTGGTCTGTTGACGAGAAACTGCTTATCTATGATTATATCCCAAATGGTGACCTTGCTACTGCAATTCATGGAAAACCTGGAGTGGTATCCTTCACACCTCTTTCTTGGCCTGCACGTCTGAAAATCATGAATGGCTTCGCAAAAGGATTGGCTTATATTCATGAATTTAGTCCCAGAAGATATGTGCATGGAAACTTGGGGCCTAGAAACATACTGCTTGGACAAAACATGGAACCCCATATCTCCGACTTTGGACTAGGCCGCCTTGCTAACTTAACTGAAGAGTCCTTGTCATCATCGTCATTTCAACTGGAACAAATAATGGCTGAAACACCACCTCAGAATTCTCCATATGTGCAAAGGACATCGAGCTCACTAGTGGCACCCGGACCTTGCTATAAAGCTCCTGAAGCATCAAAAGTAACCAAGCCATCACAGAAATGGGATGTTTACTCATTTGGGGTAGTTATACTGGAGATGATTTCTGCCAAGATGCCATTTATGAGAATTGGTTCTTTGGAAATGGATTTGATACAACAGTTTCAGCTCCACGTTGAGGAAAGGAAGCCACTCTGCGATCTGTTAGACCCGTTCCTAGCTCCTGATGTAGACATGAAAGAGGAGATTGTTGCGGTGCTGAAAATCGCTCTTGCTTGTGTTCATAAAAGCCCTGAAAAGAGACCTTCTATGAGGTTTGTGTGTGATAATTTGGCCAGGTTGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

701

Amino Acids

76.57

Weight (kDa)

8.34

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 62 4.8e-14 Leucine rich repeat N-terminal domain
LRR_8 PF13855 67 - 125 1.6e-07 Leucine rich repeat
LRR_14 PF23598 128 - 214 2.8e-10 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 402 - 555 4.8e-19 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 402 - 693 3.3e-24 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 591 - 694 9.1e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1147
Acc36I ACCTGC 3 cut(s) 423, 1022, 1147
AccB1I GGYRCC 1 cut(s) 1759
AccB7I CCANNNNNTGG 2 cut(s) 352, 761
AccI GTMKAC 2 cut(s) 714, 1986
AccIII TCCGGA 2 cut(s) 305, 362
AciI CCGC 5 cut(s) 267, 597, 683, 1639, 2009
AclWI GGATC 3 cut(s) 487, 1158, 1226
AcoI YGGCCR 1 cut(s) 2088
AcuI CTGAAG 2 cut(s) 1677, 1806
AcyI GRCGYC 1 cut(s) 951
AfaI GTAC 6 cut(s) 136, 892, 998, 1012, 1113, 1232
AfiI CCNNNNNNNGG 4 cut(s) 352, 692, 761, 1933
AhlI ACTAGT 1 cut(s) 1753
AjiI CACGTC 1 cut(s) 1493
AjnI CCWGG 3 cut(s) 137, 1453, 2090
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
Alw21I GWGCWC 4 cut(s) 253, 345, 769, 1751
Alw26I GTCTC 4 cut(s) 478, 660, 701, 2050
AlwI GGATC 3 cut(s) 487, 1158, 1226
Aor13HI TCCGGA 2 cut(s) 305, 362
AoxI GGCC 5 cut(s) 1484, 1577, 1636, 2088, 2097
ApeKI GCWGC 3 cut(s) 882, 944, 1041
Asp700I GAANNNNTTC 2 cut(s) 1511, 1888
AspA2I CCTAGG 1 cut(s) 691
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 4 cut(s) 746, 782, 1577, 1766
AsuC2I CCSGG 1 cut(s) 1764
AsuHPI GGTGA 2 cut(s) 1171, 1436
AvaII GGWCC 3 cut(s) 746, 782, 1766
AvrII CCTAGG 1 cut(s) 691
AxyI CCTNAGG 1 cut(s) 609
BaeI ACNNNNGTAYC 2 cut(s) 1901, 1934
BalI TGGCCA 1 cut(s) 2090
BanI GGYRCC 1 cut(s) 1759
BanII GRGCYC 2 cut(s) 345, 1751
BarI GAAGNNNNNNTAC 2 cut(s) 395, 427
BauI CACGAG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 1254
Bbv12I GWGCWC 4 cut(s) 253, 345, 769, 1751
BbvI GCAGC 3 cut(s) 894, 931, 1028
BccI CCATC 4 cut(s) 285, 694, 1277, 1816
BceAI ACGGC 3 cut(s) 301, 804, 808
BciT130I CCWGG 3 cut(s) 139, 1455, 2092
BciVI GTATCC 2 cut(s) 1099, 1474
BclI TGATCA 1 cut(s) 771
BcnI CCSGG 1 cut(s) 1764
BcoDI GTCTC 4 cut(s) 478, 660, 701, 2050
BcuI ACTAGT 1 cut(s) 1753
BfaI CTAG 6 cut(s) 692, 1581, 1634, 1754, 1973, 2101
BfmI CTRYAG 3 cut(s) 468, 945, 1154
BfuAI ACCTGC 3 cut(s) 423, 1022, 1147
BfuI GTATCC 2 cut(s) 1099, 1474
BglI GCCNNNNNGGC 1 cut(s) 2096
BglII AGATCT 2 cut(s) 427, 1168
BisI GCNGC 4 cut(s) 883, 945, 1042, 1639
BlnI CCTAGG 1 cut(s) 691
BlsI GCNGC 4 cut(s) 884, 946, 1043, 1640
Bme1390I CCNGG 4 cut(s) 139, 1455, 1764, 2092
Bme18I GGWCC 3 cut(s) 746, 782, 1766
BmgBI CACGTC 1 cut(s) 1493
BmgT120I GGNCC 4 cut(s) 746, 782, 1577, 1766
BmiI GGNNCC 6 cut(s) 260, 526, 1291, 1578, 1611, 1761
BmrFI CCNGG 4 cut(s) 139, 1455, 1764, 2092
BmsI GCATC 3 cut(s) 1354, 1799, 1860
BpiI GAAGAC 1 cut(s) 1254
BpmI CTGGAG 3 cut(s) 329, 1476, 1871
BpuEI CTTGAG 1 cut(s) 898
BpuMI CCSGG 1 cut(s) 1764
BsaAI YACGTR 1 cut(s) 1025
BsaHI GRCGYC 1 cut(s) 951
BsaI GGTCTC 1 cut(s) 2050
BsaJI CCNNGG 1 cut(s) 691
BsaWI WCCGGW 2 cut(s) 305, 362
BsaXI ACNNNNNCTCC 2 cut(s) 287, 317
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 692, 761, 1933
Bse1I ACTGG 3 cut(s) 998, 1692, 1854
Bse21I CCTNAGG 1 cut(s) 609
BseAI TCCGGA 2 cut(s) 305, 362
BseBI CCWGG 3 cut(s) 139, 1455, 2092
BseDI CCNNGG 1 cut(s) 691
BseGI GGATG 3 cut(s) 686, 1345, 1828
BseLI CCNNNNNNNGG 4 cut(s) 352, 692, 761, 1933
BseNI ACTGG 3 cut(s) 998, 1692, 1854
BseRI GAGGAG 1 cut(s) 2012
BseXI GCAGC 3 cut(s) 894, 931, 1028
BseYI CCCAGC 1 cut(s) 974
BsgI GTGCAG 1 cut(s) 1473
BshFI GGCC 5 cut(s) 1486, 1579, 1638, 2090, 2099
BshNI GGYRCC 1 cut(s) 1759
BsiHKAI GWGCWC 4 cut(s) 253, 345, 769, 1751
BsiSI CCGG 3 cut(s) 306, 363, 1764
BslFI GGGAC 1 cut(s) 1533
BslI CCNNNNNNNGG 4 cut(s) 352, 692, 761, 1933
BsmAI GTCTC 4 cut(s) 478, 660, 701, 2050
BsmFI GGGAC 1 cut(s) 1533
BsmI GAATGC 1 cut(s) 213
BsnI GGCC 5 cut(s) 1486, 1579, 1638, 2090, 2099
Bso31I GGTCTC 1 cut(s) 2050
Bsp1286I GDGCHC 4 cut(s) 253, 345, 769, 1751
Bsp13I TCCGGA 2 cut(s) 305, 362
Bsp1407I TGTACA 1 cut(s) 1230
Bsp143I GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
BspACI CCGC 5 cut(s) 267, 597, 683, 1639, 2009
BspANI GGCC 5 cut(s) 1486, 1579, 1638, 2090, 2099
BspEI TCCGGA 2 cut(s) 305, 362
BspHI TCATGA 2 cut(s) 1503, 1537
BspLI GGNNCC 6 cut(s) 260, 526, 1291, 1578, 1611, 1761
BspMAI CTGCAG 2 cut(s) 949, 1158
BspMI ACCTGC 3 cut(s) 423, 1022, 1147
BspPI GGATC 3 cut(s) 487, 1158, 1226
BspQI GCTCTTC 1 cut(s) 847
BspT107I GGYRCC 1 cut(s) 1759
BspTNI GGTCTC 1 cut(s) 2050
BsrGI TGTACA 1 cut(s) 1230
BsrI ACTGG 3 cut(s) 998, 1692, 1854
BssECI CCNNGG 1 cut(s) 691
BssMI GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
BssNAI GTATAC 1 cut(s) 715
BssNI GRCGYC 1 cut(s) 951
BssSI CACGAG 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 691
Bst1107I GTATAC 1 cut(s) 715
Bst2BI CACGAG 1 cut(s) 246
Bst2UI CCWGG 3 cut(s) 139, 1455, 2092
Bst4CI ACNGT 6 cut(s) 674, 712, 851, 940, 1111, 1917
Bst6I CTCTTC 2 cut(s) 847, 1653
BstACI GRCGYC 1 cut(s) 951
BstAUI TGTACA 1 cut(s) 1230
BstBAI YACGTR 1 cut(s) 1025
BstC8I GCNNGC 5 cut(s) 314, 701, 949, 1039, 1488
BstEII GGTNACC 1 cut(s) 1424
BstF5I GGATG 3 cut(s) 686, 1345, 1828
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 9 cut(s) 430, 495, 641, 724, 774, 1153, 1171, 1221, 1957
BstMAI GTCTC 4 cut(s) 478, 660, 701, 2050
BstMBI GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
BstMWI GCNNNNNNNGC 3 cut(s) 322, 1787, 2096
BstNI CCWGG 3 cut(s) 139, 1455, 2092
BstNSI RCATGY 1 cut(s) 186
BstPI GGTNACC 1 cut(s) 1424
BstSCI CCNGG 4 cut(s) 137, 1453, 1762, 2090
BstSFI CTRYAG 3 cut(s) 468, 945, 1154
BstV1I GCAGC 3 cut(s) 894, 931, 1028
BstV2I GAAGAC 1 cut(s) 1254
BstX2I RGATCY 4 cut(s) 427, 492, 1150, 1168
BstXI CCANNNNNNTGG 2 cut(s) 168, 521
BstYI RGATCY 4 cut(s) 427, 492, 1150, 1168
BstZ17I GTATAC 1 cut(s) 715
Bsu36I CCTNAGG 1 cut(s) 609
BsuI GTATCC 2 cut(s) 1099, 1474
BsuRI GGCC 5 cut(s) 1486, 1579, 1638, 2090, 2099
BtgZI GCGATG 1 cut(s) 940
BtrI CACGTC 1 cut(s) 1493
BtsCI GGATG 3 cut(s) 686, 1345, 1828
BtsI GCAGTG 1 cut(s) 1272
BtsIMutI CAGTG 4 cut(s) 482, 748, 755, 1272
BveI ACCTGC 3 cut(s) 423, 1022, 1147
Cac8I GCNNGC 5 cut(s) 314, 701, 949, 1039, 1488
CciI TCATGA 2 cut(s) 1503, 1537
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 4 cut(s) 746, 782, 1577, 1766
CseI GACGC 1 cut(s) 940
Csp6I GTAC 6 cut(s) 135, 891, 997, 1011, 1112, 1231
CspCI CAANNNNNGTGG 2 cut(s) 724, 759
CviQI GTAC 6 cut(s) 135, 891, 997, 1011, 1112, 1231
DpnI GATC 9 cut(s) 429, 494, 640, 723, 773, 1152, 1170, 1220, 1956
DpnII GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
EaeI YGGCCR 1 cut(s) 2088
Eam1104I CTCTTC 2 cut(s) 847, 1653
EarI CTCTTC 2 cut(s) 847, 1653
EciI GGCGGA 2 cut(s) 256, 672
Ecl136II GAGCTC 2 cut(s) 343, 1749
Eco130I CCWWGG 1 cut(s) 691
Eco24I GRGCYC 2 cut(s) 345, 1751
Eco31I GGTCTC 1 cut(s) 2050
Eco47I GGWCC 3 cut(s) 746, 782, 1766
Eco53kI GAGCTC 2 cut(s) 343, 1749
Eco57I CTGAAG 2 cut(s) 1677, 1806
Eco81I CCTNAGG 1 cut(s) 609
Eco91I GGTNACC 1 cut(s) 1424
EcoICRI GAGCTC 2 cut(s) 343, 1749
EcoO109I RGGNCCY 1 cut(s) 1577
EcoO65I GGTNACC 1 cut(s) 1424
EcoRI GAATTC 1 cut(s) 1720
EcoRII CCWGG 3 cut(s) 137, 1453, 2090
EcoT14I CCWWGG 1 cut(s) 691
EcoT38I GRGCYC 2 cut(s) 345, 1751
ErhI CCWWGG 1 cut(s) 691
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 1533
FauNDI CATATG 1 cut(s) 1729
FbaI TGATCA 1 cut(s) 771
FblI GTMKAC 2 cut(s) 714, 1986
Fnu4HI GCNGC 4 cut(s) 883, 945, 1042, 1639
FokI GGATG 3 cut(s) 673, 1332, 1835
FriOI GRGCYC 2 cut(s) 345, 1751
Fsp4HI GCNGC 4 cut(s) 883, 945, 1042, 1639
FspBI CTAG 6 cut(s) 692, 1581, 1634, 1754, 1973, 2101
GlaI GCGC 1 cut(s) 275
GluI GCNGC 4 cut(s) 883, 945, 1042, 1639
GsaI CCCAGC 1 cut(s) 978
GsuI CTGGAG 3 cut(s) 329, 1476, 1871
HaeIII GGCC 5 cut(s) 1486, 1579, 1638, 2090, 2099
HapII CCGG 3 cut(s) 306, 363, 1764
HgaI GACGC 1 cut(s) 940
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 951
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 1387
HindII GTYRAC 1 cut(s) 1387
HindIII AAGCTT 3 cut(s) 310, 697, 856
HinfI GANTC 7 cut(s) 101, 283, 546, 604, 751, 1360, 1661
HpaII CCGG 3 cut(s) 306, 363, 1764
HphI GGTGA 2 cut(s) 1171, 1436
Hpy166II GTNNAC 9 cut(s) 715, 746, 809, 818, 1028, 1231, 1387, 1828, 1987
Hpy188I TCNGA 9 cut(s) 132, 151, 282, 322, 771, 1070, 1497, 1624, 1719
Hpy8I GTNNAC 9 cut(s) 715, 746, 809, 818, 1028, 1231, 1387, 1828, 1987
HpyAV CCTTC 8 cut(s) 68, 471, 539, 592, 997, 1057, 1477, 2070
HpyCH4III ACNGT 6 cut(s) 674, 712, 851, 940, 1111, 1917
HpyCH4IV ACGT 3 cut(s) 1024, 1492, 1929
HpyF10VI GCNNNNNNNGC 3 cut(s) 322, 1787, 2096
HpySE526I ACGT 3 cut(s) 1024, 1492, 1929
Hsp92I GRCGYC 1 cut(s) 951
HspAI GCGC 1 cut(s) 274
Kpn2I TCCGGA 2 cut(s) 305, 362
Ksp22I TGATCA 1 cut(s) 771
Kzo9I GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 6 cut(s) 348, 530, 1126, 1786, 1929, 1981
Lsp1109I GCAGC 3 cut(s) 894, 931, 1028
LweI GCATC 3 cut(s) 1354, 1799, 1860
MaeI CTAG 6 cut(s) 692, 1581, 1634, 1754, 1973, 2101
MaeII ACGT 3 cut(s) 1024, 1492, 1929
MaeIII GTNAC 5 cut(s) 178, 410, 728, 1424, 1798
MalI GATC 9 cut(s) 429, 494, 640, 723, 773, 1152, 1170, 1220, 1956
MboI GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
MflI RGATCY 4 cut(s) 427, 492, 1150, 1168
MhlI GDGCHC 4 cut(s) 253, 345, 769, 1751
MlsI TGGCCA 1 cut(s) 2090
MluNI TGGCCA 1 cut(s) 2090
MlyI GAGTC 1 cut(s) 1670
MmeI TCCRAC 4 cut(s) 154, 1014, 1048, 1647
Mox20I TGGCCA 1 cut(s) 2090
MroI TCCGGA 2 cut(s) 305, 362
MroXI GAANNNNTTC 2 cut(s) 1511, 1888
MscI TGGCCA 1 cut(s) 2090
MseI TTAA 3 cut(s) 107, 860, 1652
MslI CAYNNNNRTG 2 cut(s) 166, 1816
Msp20I TGGCCA 1 cut(s) 2090
MspA1I CMGCKG 2 cut(s) 392, 597
MspI CCGG 3 cut(s) 306, 363, 1764
MspR9I CCNGG 4 cut(s) 139, 1455, 1764, 2092
Mva1269I GAATGC 1 cut(s) 213
MvaI CCWGG 3 cut(s) 139, 1455, 2092
MwoI GCNNNNNNNGC 3 cut(s) 322, 1787, 2096
NciI CCSGG 1 cut(s) 1764
NdeI CATATG 1 cut(s) 1729
NdeII GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
NlaIV GGNNCC 6 cut(s) 260, 526, 1291, 1578, 1611, 1761
NmuCI GTSAC 1 cut(s) 1424
NspI RCATGY 1 cut(s) 186
PagI TCATGA 2 cut(s) 1503, 1537
PaqCI CACCTGC 1 cut(s) 1147
PciSI GCTCTTC 1 cut(s) 847
PctI GAATGC 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 1511, 1888
PfeI GAWTC 6 cut(s) 101, 283, 546, 604, 751, 1360
PflMI CCANNNNNTGG 2 cut(s) 352, 761
PkrI GCNGC 4 cut(s) 884, 946, 1043, 1640
PleI GAGTC 1 cut(s) 1669
PpsI GAGTC 1 cut(s) 1669
Ppu21I YACGTR 1 cut(s) 1025
Psp124BI GAGCTC 2 cut(s) 345, 1751
Psp6I CCWGG 3 cut(s) 137, 1453, 2090
PspEI GGTNACC 1 cut(s) 1424
PspFI CCCAGC 1 cut(s) 974
PspGI CCWGG 3 cut(s) 137, 1453, 2090
PspN4I GGNNCC 6 cut(s) 260, 526, 1291, 1578, 1611, 1761
PspPI GGNCC 4 cut(s) 746, 782, 1577, 1766
PstI CTGCAG 2 cut(s) 949, 1158
PsuI RGATCY 4 cut(s) 427, 492, 1150, 1168
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 6 cut(s) 136, 892, 998, 1012, 1113, 1232
RsaNI GTAC 6 cut(s) 135, 891, 997, 1011, 1112, 1231
RseI CAYNNNNRTG 2 cut(s) 166, 1816
SacI GAGCTC 2 cut(s) 345, 1751
SapI GCTCTTC 1 cut(s) 847
SaqAI TTAA 3 cut(s) 107, 860, 1652
SatI GCNGC 4 cut(s) 883, 945, 1042, 1639
Sau3AI GATC 9 cut(s) 427, 492, 638, 721, 771, 1150, 1168, 1218, 1954
Sau96I GGNCC 4 cut(s) 746, 782, 1577, 1766
SchI GAGTC 1 cut(s) 1670
ScrFI CCNGG 4 cut(s) 139, 1455, 1764, 2092
SduI GDGCHC 4 cut(s) 253, 345, 769, 1751
SfaNI GCATC 3 cut(s) 1354, 1799, 1860
SfcI CTRYAG 3 cut(s) 468, 945, 1154
SfiI GGCCNNNNNGGCC 1 cut(s) 2096
SinI GGWCC 3 cut(s) 746, 782, 1766
SmiMI CAYNNNNRTG 2 cut(s) 166, 1816
SmlI CTYRAG 1 cut(s) 913
SmoI CTYRAG 1 cut(s) 913
SpeI ACTAGT 1 cut(s) 1753
SsiI CCGC 5 cut(s) 267, 597, 683, 1639, 2009
SspI AATATT 3 cut(s) 577, 589, 1354
SspMI CTAG 6 cut(s) 692, 1581, 1634, 1754, 1973, 2101
SstI GAGCTC 2 cut(s) 345, 1751
StyD4I CCNGG 4 cut(s) 137, 1453, 1762, 2090
StyI CCWWGG 1 cut(s) 691
TaaI ACNGT 6 cut(s) 674, 712, 851, 940, 1111, 1917
TaiI ACGT 3 cut(s) 1027, 1495, 1932
TaqI TCGA 3 cut(s) 455, 602, 1745
TaqII GACCGA 1 cut(s) 763
TatI WGTACW 1 cut(s) 1230
TauI GCSGC 1 cut(s) 1641
TfiI GAWTC 6 cut(s) 101, 283, 546, 604, 751, 1360
Tru1I TTAA 3 cut(s) 107, 860, 1652
Tru9I TTAA 3 cut(s) 107, 860, 1652
TscAI CASTG 4 cut(s) 482, 748, 762, 1272
TseFI GTSAC 1 cut(s) 1424
TseI GCWGC 3 cut(s) 882, 944, 1041
Tsp45I GTSAC 1 cut(s) 1424
TspGWI ACGGA 1 cut(s) 1266
TspRI CASTG 4 cut(s) 482, 748, 762, 1272
Van91I CCANNNNNTGG 2 cut(s) 352, 761
VpaK11BI GGWCC 3 cut(s) 746, 782, 1766
XceI RCATGY 1 cut(s) 186
XcmI CCANNNNNNNNNTGG 1 cut(s) 1815
XmaJI CCTAGG 1 cut(s) 691
XmiI GTMKAC 2 cut(s) 714, 1986
XmnI GAANNNNTTC 2 cut(s) 1511, 1888
XspI CTAG 6 cut(s) 692, 1581, 1634, 1754, 1973, 2101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.