RchiOBHm_Chr2g0123721
NAC Family

Inactive leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0004524
Physical Location & Seq
Forward (+)
37001600 .. 37002897
1298 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49599

Sequence Viewer

Length: 1257 bp
ATGGTTTTGCGAGTGCTTCTCCTGGTTTTGCTTGTATTCAACTCTCTTGCATTTGTAAGCTGTGTAAATGAGGAAGGTTTAGCACTACTTTCGTTCAAAGGATCCTTAACTGAGTATGCCGAAGGCTCTATGAATAACTGGAACTCTTCAGACCAGAGCCCATGTTCATGGGATGGAGTCACATGTAAACAAGAGAAAGTTGTATCTCTCAGTCTTCCAAACAAAAATCTTTCCGGGATTTTCCCTTCTGCTCTTGGGAACCTCTCTGCCCTCTGCCGTCTGAATCTCAGGAACAACAGGCTTTTGGGAAGACTGCCATTAGATTTGTTCCATGCAAAGGAGCTCCAGAGTGTGGTACTATCATCAAACTCATTATCCGGGTCTGTTCCAGCTGAGATACAGATGCTCAGTTCCCTACATACACTAGATCTCTCACAAAATTCATTCAATAACTCCATACCTTTATCTATAGCTCGGTGCAGGAGACTAAAGAGTATTGTCCTTGCTCAAAACCATTTCTCTGGTTCCCTTCCAGATGGTATCACCAGCTTGGCAAGTCTTCAAAAGCTCAACATTTCCTTCAACAGGCTCAGCGGTTCCATCCCCAAGGGCATTAGCAACTTGTCGACTTTGAGAGCAACTCTCGACATGTCTCACAACTTGTTTAATGGTTCTATACCAGCAAGCCTAGGAAACCTTCCTCAGAGAGTTTATATTGATCTCAGTTACAACAATCTAAGTGGACCAATTCCACAGATTGGATCTCTTATCAATCTAGGACCAACAGCTTTTGCAGGCAATCTTTTTCTCTGTGGACCGCCATTGAAGACCTCATGTTGTTCAAGCACTCAAGACTCAAATTGTCAATCCTTGTTTCACAATCCGCCTCGTAATTCAGGTCGGAGCAGCAACTCTAGTACATGTGCTGTGGTCTTTGTCATTGCAGGTATCATTATTGGGATTTGTCTTTCTGGTCTATTGTTCTCCAAATGGTATAAGAAGATCTGTGCTTGTAAAGTCAGTCGACATTTTGAACAGACACTCATGCCCACGAAGGACTTTCTCTACATTGCTAAACACGGTTCAGATTATCCGGAGCATTACCAATTTGTGCAATTGGACCTGCATGTCAGTTTAGATCTTGACAAACTGCTGAAAGCCTCTGCTTTCTTCCTTGGTAAGAGTAAAACTGGGATTGTGTATAAAGTTGTCCTTGAAGATGGACGAACCTTGTGTTGGGATCCCTCCTTATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

418

Amino Acids

45.45

Weight (kDa)

8.92

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 23 - 62 1.7e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 127 - 213 2e-10 Leucine-rich repeat region
LRR_8 PF13855 138 - 197 6.3e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1127
Acc36I ACCTGC 2 cut(s) 935, 1131
AccB7I CCANNNNNTGG 2 cut(s) 352, 758
AccI GTMKAC 2 cut(s) 626, 1024
AccIII TCCGGA 1 cut(s) 1093
AciI CCGC 3 cut(s) 594, 818, 884
AclWI GGATC 5 cut(s) 96, 109, 769, 1235, 1248
AcsI RAATTY 1 cut(s) 439
AcuI CTGAAG 1 cut(s) 132
AfaI GTAC 2 cut(s) 357, 919
AfiI CCNNNNNNNGG 5 cut(s) 337, 352, 585, 758, 1236
AflIII ACRYGT 3 cut(s) 182, 648, 920
AgsI TTSAA 9 cut(s) 40, 97, 448, 563, 583, 826, 843, 1034, 1217
AjnI CCWGG 1 cut(s) 21
AjuI GAANNNNNNNTTGG 2 cut(s) 1219, 1251
AluBI AGCT 7 cut(s) 60, 343, 392, 473, 549, 568, 788
AluI AGCT 7 cut(s) 60, 343, 392, 473, 549, 568, 788
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 478, 657
AlwI GGATC 5 cut(s) 96, 109, 769, 1235, 1248
Aor13HI TCCGGA 1 cut(s) 1093
ApeKI GCWGC 1 cut(s) 906
ApoI RAATTY 1 cut(s) 439
AspA2I CCTAGG 1 cut(s) 688
AspS9I GGNCC 4 cut(s) 743, 779, 815, 1120
AsuC2I CCSGG 2 cut(s) 235, 379
AsuHPI GGTGA 1 cut(s) 535
AvaII GGWCC 4 cut(s) 743, 779, 815, 1120
AvrII CCTAGG 1 cut(s) 688
BamHI GGATCC 2 cut(s) 101, 1240
BanII GRGCYC 2 cut(s) 161, 345
BbsI GAAGAC 4 cut(s) 206, 316, 551, 833
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 1 cut(s) 918
BccI CCATC 4 cut(s) 167, 530, 608, 1214
BceAI ACGGC 1 cut(s) 261
BcgI CGANNNNNNTGC 2 cut(s) 72, 106
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 2 cut(s) 235, 379
BcoDI GTCTC 2 cut(s) 478, 657
BfaI CTAG 4 cut(s) 425, 689, 776, 915
BfmI CTRYAG 1 cut(s) 468
BfuAI ACCTGC 2 cut(s) 935, 1131
BglII AGATCT 3 cut(s) 427, 1002, 1138
BisI GCNGC 1 cut(s) 907
BlnI CCTAGG 1 cut(s) 688
BlpI GCTNAGC 1 cut(s) 590
BlsI GCNGC 1 cut(s) 908
Bme1390I CCNGG 3 cut(s) 23, 235, 379
Bme18I GGWCC 4 cut(s) 743, 779, 815, 1120
BmgT120I GGNCC 4 cut(s) 743, 779, 815, 1120
BmiI GGNNCC 5 cut(s) 103, 260, 526, 598, 1242
BmrFI CCNGG 3 cut(s) 23, 235, 379
BmrI ACTGGG 1 cut(s) 1200
BmsI GCATC 1 cut(s) 393
BmuI ACTGGG 1 cut(s) 1200
BpiI GAAGAC 4 cut(s) 206, 316, 551, 833
BplI GAGNNNNNCTC 5 cut(s) 35, 625, 657, 627, 659
BpmI CTGGAG 1 cut(s) 329
Bpu1102I GCTNAGC 1 cut(s) 590
BpuEI CTTGAG 1 cut(s) 834
BpuMI CCSGG 2 cut(s) 235, 379
BsaJI CCNNGG 3 cut(s) 606, 688, 1174
BsaWI WCCGGW 1 cut(s) 1093
Bsc4I CCNNNNNNNGG 5 cut(s) 337, 352, 585, 758, 1236
Bse1I ACTGG 2 cut(s) 143, 1195
Bse3DI GCAATG 2 cut(s) 939, 1068
BseAI TCCGGA 1 cut(s) 1093
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 3 cut(s) 606, 688, 1174
BseGI GGATG 2 cut(s) 178, 600
BseLI CCNNNNNNNGG 5 cut(s) 337, 352, 585, 758, 1236
BseMI GCAATG 2 cut(s) 939, 1068
BseMII CTCAG 8 cut(s) 102, 223, 301, 384, 421, 604, 716, 736
BseNI ACTGG 2 cut(s) 143, 1195
BseXI GCAGC 1 cut(s) 918
BsgI GTGCAG 1 cut(s) 499
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 3 cut(s) 234, 378, 1094
BslI CCNNNNNNNGG 5 cut(s) 337, 352, 585, 758, 1236
BsmAI GTCTC 2 cut(s) 478, 657
Bsp1286I GDGCHC 2 cut(s) 161, 345
Bsp13I TCCGGA 1 cut(s) 1093
Bsp143I GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
Bsp1720I GCTNAGC 1 cut(s) 590
BspACI CCGC 3 cut(s) 594, 818, 884
BspCNI CTCAG 8 cut(s) 103, 222, 300, 385, 420, 603, 715, 735
BspEI TCCGGA 1 cut(s) 1093
BspLI GGNNCC 5 cut(s) 103, 260, 526, 598, 1242
BspMI ACCTGC 2 cut(s) 935, 1131
BspPI GGATC 5 cut(s) 96, 109, 769, 1235, 1248
BsrDI GCAATG 2 cut(s) 939, 1068
BsrI ACTGG 2 cut(s) 143, 1195
BssECI CCNNGG 3 cut(s) 606, 688, 1174
BssMI GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
BssT1I CCWWGG 3 cut(s) 606, 688, 1174
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 1082
Bst6I CTCTTC 1 cut(s) 151
BstC8I GCNNGC 2 cut(s) 685, 796
BstDEI CTNAG 9 cut(s) 111, 209, 287, 393, 407, 590, 702, 722, 737
BstENI CCTNNNNNAGG 1 cut(s) 583
BstF5I GGATG 2 cut(s) 178, 600
BstKTI GATC 7 cut(s) 104, 430, 721, 764, 1005, 1141, 1243
BstMAI GTCTC 2 cut(s) 478, 657
BstMBI GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
BstNI CCWGG 1 cut(s) 23
BstNSI RCATGY 4 cut(s) 186, 652, 924, 1130
BstSCI CCNGG 3 cut(s) 21, 233, 377
BstSFI CTRYAG 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 918
BstV2I GAAGAC 4 cut(s) 206, 316, 551, 833
BstX2I RGATCY 6 cut(s) 101, 427, 761, 1002, 1138, 1240
BstXI CCANNNNNNTGG 2 cut(s) 168, 521
BstYI RGATCY 6 cut(s) 101, 427, 761, 1002, 1138, 1240
BtsCI GGATG 2 cut(s) 178, 600
BveI ACCTGC 2 cut(s) 935, 1131
Cac8I GCNNGC 2 cut(s) 685, 796
Cfr13I GGNCC 4 cut(s) 743, 779, 815, 1120
Csp6I GTAC 2 cut(s) 356, 918
CspCI CAANNNNNGTGG 2 cut(s) 721, 756
CviAII CATG 9 cut(s) 162, 168, 183, 332, 649, 834, 921, 1045, 1127
CviQI GTAC 2 cut(s) 356, 918
DdeI CTNAG 9 cut(s) 111, 209, 287, 393, 407, 590, 702, 722, 737
DpnI GATC 7 cut(s) 103, 429, 720, 763, 1004, 1140, 1242
DpnII GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
DrdI GACNNNNNNGTC 1 cut(s) 1127
DseDI GACNNNNNNGTC 1 cut(s) 1127
Eam1104I CTCTTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 151
EciI GGCGGA 1 cut(s) 873
Ecl136II GAGCTC 1 cut(s) 343
Eco130I CCWWGG 3 cut(s) 606, 688, 1174
Eco24I GRGCYC 2 cut(s) 161, 345
Eco47I GGWCC 4 cut(s) 743, 779, 815, 1120
Eco53kI GAGCTC 1 cut(s) 343
Eco57I CTGAAG 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 343
EcoNI CCTNNNNNAGG 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 21
EcoT14I CCWWGG 3 cut(s) 606, 688, 1174
EcoT38I GRGCYC 2 cut(s) 161, 345
ErhI CCWWGG 3 cut(s) 606, 688, 1174
FaeI CATG 9 cut(s) 165, 171, 186, 335, 652, 837, 924, 1048, 1130
FalI AAGNNNNNCTT 2 cut(s) 1197, 1229
FatI CATG 9 cut(s) 161, 167, 182, 331, 648, 833, 920, 1044, 1126
FblI GTMKAC 2 cut(s) 626, 1024
Fnu4HI GCNGC 1 cut(s) 907
FokI GGATG 2 cut(s) 185, 587
FriOI GRGCYC 2 cut(s) 161, 345
Fsp4HI GCNGC 1 cut(s) 907
FspBI CTAG 4 cut(s) 425, 689, 776, 915
GluI GCNGC 1 cut(s) 907
GsuI CTGGAG 1 cut(s) 329
HapII CCGG 3 cut(s) 234, 378, 1094
Hin1II CATG 9 cut(s) 165, 171, 186, 335, 652, 837, 924, 1048, 1130
HincII GTYRAC 2 cut(s) 627, 1025
HindII GTYRAC 2 cut(s) 627, 1025
HinfI GANTC 3 cut(s) 177, 283, 854
HpaII CCGG 3 cut(s) 234, 378, 1094
HphI GGTGA 1 cut(s) 535
Hpy166II GTNNAC 5 cut(s) 188, 627, 743, 815, 1025
Hpy188I TCNGA 5 cut(s) 151, 282, 705, 903, 1087
Hpy188III TCNNGA 7 cut(s) 289, 346, 533, 644, 851, 1094, 1142
Hpy8I GTNNAC 5 cut(s) 188, 627, 743, 815, 1025
HpyAV CCTTC 7 cut(s) 68, 116, 255, 539, 589, 707, 1048
HpyCH4III ACNGT 1 cut(s) 1082
HpyCH4V TGCA 7 cut(s) 50, 335, 480, 794, 944, 1114, 1126
HpyF3I CTNAG 9 cut(s) 111, 209, 287, 393, 407, 590, 702, 722, 737
Hsp92II CATG 9 cut(s) 165, 171, 186, 335, 652, 837, 924, 1048, 1130
Kpn2I TCCGGA 1 cut(s) 1093
Kzo9I GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
LmnI GCTCC 4 cut(s) 340, 348, 903, 1096
Lsp1109I GCAGC 1 cut(s) 918
LweI GCATC 1 cut(s) 393
MaeI CTAG 4 cut(s) 425, 689, 776, 915
MaeIII GTNAC 2 cut(s) 178, 725
MalI GATC 7 cut(s) 103, 429, 720, 763, 1004, 1140, 1242
MboI GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
MboII GAAGA 8 cut(s) 138, 206, 321, 551, 838, 1012, 1162, 1229
MfeI CAATTG 1 cut(s) 1115
MflI RGATCY 6 cut(s) 101, 427, 761, 1002, 1138, 1240
MhlI GDGCHC 2 cut(s) 161, 345
MluCI AATT 6 cut(s) 439, 747, 859, 892, 1106, 1115
MlyI GAGTC 2 cut(s) 186, 848
MmeI TCCRAC 1 cut(s) 881
MnlI CCTC 8 cut(s) 64, 272, 281, 711, 841, 897, 1171, 1255
MroI TCCGGA 1 cut(s) 1093
MseI TTAA 2 cut(s) 107, 666
MslI CAYNNNNRTG 1 cut(s) 166
MspA1I CMGCKG 2 cut(s) 392, 594
MspI CCGG 3 cut(s) 234, 378, 1094
MspR9I CCNGG 3 cut(s) 23, 235, 379
MunI CAATTG 1 cut(s) 1115
MvaI CCWGG 1 cut(s) 23
NciI CCSGG 2 cut(s) 235, 379
NdeII GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
NlaIII CATG 9 cut(s) 165, 171, 186, 335, 652, 837, 924, 1048, 1130
NlaIV GGNNCC 5 cut(s) 103, 260, 526, 598, 1242
NmuCI GTSAC 1 cut(s) 178
NspI RCATGY 4 cut(s) 186, 652, 924, 1130
PciI ACATGT 3 cut(s) 182, 648, 920
PfeI GAWTC 1 cut(s) 283
PflMI CCANNNNNTGG 2 cut(s) 352, 758
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 1 cut(s) 908
PleI GAGTC 2 cut(s) 185, 848
PpsI GAGTC 2 cut(s) 185, 848
PscI ACATGT 3 cut(s) 182, 648, 920
Psp124BI GAGCTC 1 cut(s) 345
Psp6I CCWGG 1 cut(s) 21
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 5 cut(s) 103, 260, 526, 598, 1242
PspPI GGNCC 4 cut(s) 743, 779, 815, 1120
PsuI RGATCY 6 cut(s) 101, 427, 761, 1002, 1138, 1240
PvuII CAGCTG 1 cut(s) 392
RsaI GTAC 2 cut(s) 357, 919
RsaNI GTAC 2 cut(s) 356, 918
RseI CAYNNNNRTG 1 cut(s) 166
SacI GAGCTC 1 cut(s) 345
SalI GTCGAC 2 cut(s) 625, 1023
SaqAI TTAA 2 cut(s) 107, 666
SatI GCNGC 1 cut(s) 907
Sau3AI GATC 7 cut(s) 101, 427, 718, 761, 1002, 1138, 1240
Sau96I GGNCC 4 cut(s) 743, 779, 815, 1120
SchI GAGTC 2 cut(s) 186, 848
ScrFI CCNGG 3 cut(s) 23, 235, 379
SduI GDGCHC 2 cut(s) 161, 345
SfaNI GCATC 1 cut(s) 393
SfcI CTRYAG 1 cut(s) 468
SinI GGWCC 4 cut(s) 743, 779, 815, 1120
SmiMI CAYNNNNRTG 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 849
SmoI CTYRAG 1 cut(s) 849
Sse9I AATT 6 cut(s) 439, 747, 859, 892, 1106, 1115
SsiI CCGC 3 cut(s) 594, 818, 884
SspMI CTAG 4 cut(s) 425, 689, 776, 915
SstI GAGCTC 1 cut(s) 345
StyD4I CCNGG 3 cut(s) 21, 233, 377
StyI CCWWGG 3 cut(s) 606, 688, 1174
TaaI ACNGT 1 cut(s) 1082
TaqI TCGA 3 cut(s) 626, 645, 1024
TasI AATT 6 cut(s) 439, 747, 859, 892, 1106, 1115
TatI WGTACW 1 cut(s) 917
TfiI GAWTC 1 cut(s) 283
Tru1I TTAA 2 cut(s) 107, 666
Tru9I TTAA 2 cut(s) 107, 666
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 906
Tsp45I GTSAC 1 cut(s) 178
TspDTI ATGAA 3 cut(s) 146, 156, 432
Van91I CCANNNNNTGG 2 cut(s) 352, 758
VpaK11BI GGWCC 4 cut(s) 743, 779, 815, 1120
XagI CCTNNNNNAGG 1 cut(s) 583
XapI RAATTY 1 cut(s) 439
XceI RCATGY 4 cut(s) 186, 652, 924, 1130
XmaJI CCTAGG 1 cut(s) 688
XmiI GTMKAC 2 cut(s) 626, 1024
XspI CTAG 4 cut(s) 425, 689, 776, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.