pycom01g10730

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002962
Physical Location & Seq
Forward (+)
11547529 .. 11550168
2640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2049 bp
ATGCCATGCAGTTCACTAGTTATTCCTACCTCTCCGGCTTCCGCCACGACGGTAGACGTTTTCCAAGAGCACAATGGTCTCCGCCGGTCCAACTCCAACCAAGACCTTCACCGTCGTGCTACTATGCGGAGGTCTTATTCGGACAACCATCTTTGTTACCGCATCAGTCGTATCCACGCAGCAGCAACAGAACCAAAACTAAAGAACAGCCGTTCGGGGATTTTCCCGTTTCAGTTTTCGAGTTCCATTCTCCCAAACTCGGTGAGATCATTCTCGTTTGATCCAGACACGAGTAATCATACAAGCATAAAGGCGAAGGACATGAGTAAGGAGACCTCTGGGGAGAGCGCCAGTGAGGAGGAGAGCAAAAGAGCCAATTGGGTGGAGCGACTTGTGGAAATCAGAAGTAAATGGAGGGATAGGCAGAAAAAGGACGATGCGGATGAAGACAGCGTCTGTGAAGCGGGTGAAAATGGTGATTGTGAATGTGGCAGAGATGAGGGTGGTTGTGCGGTGGATTATACTGCAGAAGACGATGGGGGAGAAACCAGTCCTGAATTTTTCCGGAGGTTTTTGGTTCGAGTTCCATGGTCTGATACCAAGCTGTTTTCGAAGCTATCCTTCTTGTGCAACTTGGCCTATGCGATGCCACAAATCAAGGAAAATTATATAAAGAGATATTATGGCCTACGATTAGTAACAACTTCACTAAAGAAGAAGGCCGAAGATGAAATCAAAGCAAAACTAGATAACGACTCGAATCGTGTACCTGAAGCTGCCGAAGTAGCTAGTGAATCCAGTTCGGAGAAAGCTGTAGAAGCTGGGCACAAGCGTCCATGCCGTCCTGCTGTTGCTTACAATATTGCTGCCTCAGCTGCATCTTATGTGCGGTCACGCAATCAGGACAGCTTCTCACTCGGGCCCAAATCGCAGCAGGAGGGTGATGGCGAAAATTCACGAGAGGAAGGAGGGTACTCACCTCGAAGCTATAAGCCAGAGGTGGCAGTTTCCATGGCAGCGTCAACAATGACAGCAGTGGTTGCAGCAGGGGAAAAGGAGAAACAAGAAACAGCAAAGGACCTTCGGTCACTCCAGTCCTCACCTTGTGAATGGTTTGTCTGTGATGATTCCAGTACGTATACTCGTTGTTTCGTGATTCAGGGTTCCGACTCCCTCGCATCCTGGCAGGCAAACCTGTTCTTCGAACCTACCAAATTCGAGGGGACAGATGTGCTTGTTCACAGAGGAATCTACGAAGCAGCGAAGGGAATGTACGAGCGATTTATTCCAGAAATAACTGAACACTTGAATAGACATGGTGAGCGAGCGAAGCTTCAGTTTACAGGCCACTCTCTTGGAGGAAGCCTTTCTCTCTTAGTCCACTTGATGCTGCTGAGTAGGAAGTGTGTAAAACCCTCTACTCTTCGGCCAGTTGTCACATTTGGCTCACCATTTGTGTTCTGTGGAGGCCAGAAAGTACTCGACAGACTAGGAGTGGATGAGGGTCAAATCCATTCCGTGATGATGCATAGAGATATCGTCCCTAGAGCCTTCTCCTGCAACTACCCTAACTATGTGGCCACACTTCTCAAGCGTTTAAATTCATTTCGGTCACACCCTTGTCTAATAAAAAATAAAATATTATATTCTCCGATGGGCAAACTATTTATTCTCCAACCTGATGAGAATGAATCTCCTCCACACCCTCTACTTCCTCCGGGGAGTGCCCTCTATGCTTTGGACAAGACCCAACGTGGATTCTCTTCAAGTGTCCTTACGACTTTCTTGAACTCTCCACATCCACTAGAAACCCTAAGTGATCCTACAGCTTATGGTTCAGACGGTACAATTCTAAGAGACCATGACTCAAGCAACTATCTAAAGGCTGTGAATGAAGTCCTAAGGAAACACACAAGGACAGCTGTTCGGAAAGTGAGAAAAGAAAAGAATCTATTGTGGCCGCTACTTACTTCACCATCTCCACACTCCTGGAACCATGAAAGTAATCTGGAGAGCAGCGGGTTGGCAACTAAGGAGATACTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

683

Amino Acids

76.13

Weight (kDa)

8.06

Isoelectric Point (pI)

54.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 384 - 521 3.5e-25 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 54, 1139
AccIII TCCGGA 1 cut(s) 564
AclWI GGATC 2 cut(s) 275, 1814
AcoI YGGCCR 3 cut(s) 1427, 1578, 1958
AcsI RAATTY 4 cut(s) 557, 952, 1214, 1600
AcuI CTGAAG 2 cut(s) 792, 1319
AdeI CACNNNGTG 1 cut(s) 1106
AfaI GTAC 6 cut(s) 768, 974, 1135, 1274, 1479, 1846
AfiI CCNNNNNNNGG 1 cut(s) 259
AgsI TTSAA 3 cut(s) 1309, 1767, 1789
AhdI GACNNNNNGTC 1 cut(s) 1084
AhlI ACTAGT 1 cut(s) 16
AjnI CCWGG 2 cut(s) 1181, 1988
AleI CACNNNNGTG 1 cut(s) 114
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 3 cut(s) 83, 326, 1851
AlwI GGATC 2 cut(s) 275, 1814
AlwNI CAGNNNCTG 1 cut(s) 456
Ama87I CYCGRG 1 cut(s) 917
Aor13HI TCCGGA 1 cut(s) 564
AoxI GGCC 9 cut(s) 636, 685, 720, 920, 1345, 1427, 1468, 1578, 1958
ApaI GGGCCC 1 cut(s) 924
ApoI RAATTY 4 cut(s) 557, 952, 1214, 1600
Asp700I GAANNNNTTC 1 cut(s) 1366
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 4 cut(s) 87, 920, 921, 1078
AsuC2I CCSGG 1 cut(s) 1719
AsuII TTCGAA 2 cut(s) 611, 1203
AvaI CYCGRG 1 cut(s) 917
AvaII GGWCC 2 cut(s) 87, 1078
AxyI CCTNAGG 1 cut(s) 1901
BaeGI GKGCMC 3 cut(s) 828, 924, 1729
BalI TGGCCA 1 cut(s) 1580
BanII GRGCYC 1 cut(s) 924
BarI GAAGNNNNNNTAC 2 cut(s) 1256, 1288
BauI CACGAG 2 cut(s) 289, 957
BbsI GAAGAC 2 cut(s) 453, 537
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 871
BccI CCATC 5 cut(s) 156, 530, 938, 1648, 1984
BceAI ACGGC 2 cut(s) 195, 825
BciT130I CCWGG 2 cut(s) 1183, 1990
BciVI GTATCC 1 cut(s) 182
BcnI CCSGG 1 cut(s) 1719
BcoDI GTCTC 3 cut(s) 83, 326, 1851
BcuI ACTAGT 1 cut(s) 16
BfaI CTAG 6 cut(s) 17, 746, 789, 1490, 1545, 1805
BfmI CTRYAG 3 cut(s) 525, 813, 1824
BfoI RGCGCY 1 cut(s) 351
BfuI GTATCC 1 cut(s) 182
BmcAI AGTACT 1 cut(s) 1479
Bme1390I CCNGG 3 cut(s) 1183, 1719, 1990
Bme18I GGWCC 2 cut(s) 87, 1078
BmeRI GACNNNNNGTC 1 cut(s) 1084
BmeT110I CYCGRG 1 cut(s) 917
BmgT120I GGNCC 4 cut(s) 87, 920, 921, 1078
BmiI GGNNCC 3 cut(s) 922, 1165, 1994
BmrFI CCNGG 3 cut(s) 1183, 1719, 1990
BmsI GCATC 7 cut(s) 171, 427, 636, 887, 1187, 1377, 1515
BpiI GAAGAC 2 cut(s) 453, 537
BpmI CTGGAG 2 cut(s) 1076, 2030
Bpu10I CCTNAGC 1 cut(s) 871
Bpu14I TTCGAA 2 cut(s) 611, 1203
BpuEI CTTGAG 2 cut(s) 1574, 1852
BpuMI CCSGG 1 cut(s) 1719
BsaAI YACGTR 1 cut(s) 1137
BsaI GGTCTC 3 cut(s) 83, 326, 1851
BsaJI CCNNGG 3 cut(s) 587, 1011, 1718
BsaWI WCCGGW 1 cut(s) 564
Bsc4I CCNNNNNNNGG 1 cut(s) 259
Bse118I RCCGGY 1 cut(s) 84
Bse1I ACTGG 6 cut(s) 351, 549, 798, 1093, 1131, 1430
Bse21I CCTNAGG 1 cut(s) 1901
BseAI TCCGGA 1 cut(s) 564
BseBI CCWGG 2 cut(s) 1183, 1990
BseDI CCNNGG 3 cut(s) 587, 1011, 1718
BseGI GGATG 4 cut(s) 448, 1178, 1504, 1798
BseLI CCNNNNNNNGG 1 cut(s) 259
BseMII CTCAG 2 cut(s) 885, 1385
BseNI ACTGG 6 cut(s) 351, 549, 798, 1093, 1131, 1430
BseRI GAGGAG 3 cut(s) 371, 374, 1686
BseSI GKGCMC 3 cut(s) 828, 924, 1729
BseYI CCCAGC 1 cut(s) 821
BshFI GGCC 9 cut(s) 638, 687, 722, 922, 1347, 1429, 1470, 1580, 1960
BsiHKAI GWGCWC 1 cut(s) 72
BsiHKCI CYCGRG 1 cut(s) 917
BsiSI CCGG 4 cut(s) 35, 85, 565, 1718
BslFI GGGAC 2 cut(s) 1237, 1526
BslI CCNNNNNNNGG 1 cut(s) 259
BsmAI GTCTC 3 cut(s) 83, 326, 1851
BsmFI GGGAC 2 cut(s) 1237, 1526
BsnI GGCC 9 cut(s) 638, 687, 722, 922, 1347, 1429, 1470, 1580, 1960
Bso31I GGTCTC 3 cut(s) 83, 326, 1851
BsoBI CYCGRG 1 cut(s) 917
Bsp119I TTCGAA 2 cut(s) 611, 1203
Bsp120I GGGCCC 1 cut(s) 920
Bsp1286I GDGCHC 4 cut(s) 72, 828, 924, 1729
Bsp13I TCCGGA 1 cut(s) 564
Bsp143I GATC 3 cut(s) 266, 280, 1819
Bsp19I CCATGG 2 cut(s) 587, 1011
BspANI GGCC 9 cut(s) 638, 687, 722, 922, 1347, 1429, 1470, 1580, 1960
BspCNI CTCAG 2 cut(s) 884, 1386
BspEI TCCGGA 1 cut(s) 564
BspLI GGNNCC 3 cut(s) 922, 1165, 1994
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 2 cut(s) 275, 1814
BspT104I TTCGAA 2 cut(s) 611, 1203
BspTNI GGTCTC 3 cut(s) 83, 326, 1851
BsrFI RCCGGY 1 cut(s) 84
BsrI ACTGG 6 cut(s) 351, 549, 798, 1093, 1131, 1430
BssAI RCCGGY 1 cut(s) 84
BssECI CCNNGG 3 cut(s) 587, 1011, 1718
BssMI GATC 3 cut(s) 266, 280, 1819
BssNAI GTATAC 1 cut(s) 1140
BssSI CACGAG 2 cut(s) 289, 957
BssT1I CCWWGG 2 cut(s) 587, 1011
Bst1107I GTATAC 1 cut(s) 1140
Bst2BI CACGAG 2 cut(s) 289, 957
Bst2UI CCWGG 2 cut(s) 1183, 1990
Bst4CI ACNGT 3 cut(s) 52, 113, 1844
Bst6I CTCTTC 2 cut(s) 1428, 1768
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstBAI YACGTR 1 cut(s) 1137
BstBI TTCGAA 2 cut(s) 611, 1203
BstC8I GCNNGC 2 cut(s) 1188, 1326
BstDEI CTNAG 7 cut(s) 871, 1375, 1394, 1814, 1853, 1901, 2031
BstDSI CCRYGG 2 cut(s) 587, 1011
BstF5I GGATG 4 cut(s) 448, 1178, 1504, 1798
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 3 cut(s) 269, 283, 1822
BstMAI GTCTC 3 cut(s) 83, 326, 1851
BstMBI GATC 3 cut(s) 266, 280, 1819
BstMWI GCNNNNNNNGC 8 cut(s) 785, 818, 872, 875, 928, 1040, 1330, 1733
BstNI CCWGG 2 cut(s) 1183, 1990
BstSCI CCNGG 3 cut(s) 1181, 1717, 1988
BstSFI CTRYAG 3 cut(s) 525, 813, 1824
BstSLI GKGCMC 3 cut(s) 828, 924, 1729
BstSNI TACGTA 1 cut(s) 1137
BstV2I GAAGAC 2 cut(s) 453, 537
BstXI CCANNNNNNTGG 3 cut(s) 382, 1355, 1989
BstZ17I GTATAC 1 cut(s) 1140
Bsu36I CCTNAGG 1 cut(s) 1901
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 9 cut(s) 638, 687, 722, 922, 1347, 1429, 1470, 1580, 1960
BtgI CCRYGG 2 cut(s) 587, 1011
BtgZI GCGATG 1 cut(s) 659
BtsCI GGATG 4 cut(s) 448, 1178, 1504, 1798
BtsI GCAGTG 1 cut(s) 1041
BtsIMutI CAGTG 2 cut(s) 358, 1041
Cac8I GCNNGC 2 cut(s) 1188, 1326
CaiI CAGNNNCTG 1 cut(s) 456
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 1 cut(s) 84
Cfr13I GGNCC 4 cut(s) 87, 920, 921, 1078
CseI GACGC 3 cut(s) 442, 821, 1008
Csp6I GTAC 6 cut(s) 767, 973, 1134, 1273, 1478, 1845
CviAII CATG 9 cut(s) 6, 322, 588, 837, 1012, 1316, 1862, 1997, 2046
CviQI GTAC 6 cut(s) 767, 973, 1134, 1273, 1478, 1845
DdeI CTNAG 7 cut(s) 871, 1375, 1394, 1814, 1853, 1901, 2031
DpnI GATC 3 cut(s) 268, 282, 1821
DpnII GATC 3 cut(s) 266, 280, 1819
DraI TTTAAA 1 cut(s) 1599
DraIII CACNNNGTG 1 cut(s) 1106
DriI GACNNNNNGTC 1 cut(s) 1084
EaeI YGGCCR 3 cut(s) 1427, 1578, 1958
Eam1104I CTCTTC 2 cut(s) 1428, 1768
Eam1105I GACNNNNNGTC 1 cut(s) 1084
EarI CTCTTC 2 cut(s) 1428, 1768
EciI GGCGGA 2 cut(s) 31, 71
Eco105I TACGTA 1 cut(s) 1137
Eco130I CCWWGG 2 cut(s) 587, 1011
Eco24I GRGCYC 1 cut(s) 924
Eco31I GGTCTC 3 cut(s) 83, 326, 1851
Eco32I GATATC 1 cut(s) 1537
Eco47I GGWCC 2 cut(s) 87, 1078
Eco57I CTGAAG 2 cut(s) 792, 1319
Eco81I CCTNAGG 1 cut(s) 1901
Eco88I CYCGRG 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 1078
EcoRII CCWGG 2 cut(s) 1181, 1988
EcoRV GATATC 1 cut(s) 1537
EcoT14I CCWWGG 2 cut(s) 587, 1011
EcoT22I ATGCAT 1 cut(s) 1530
EcoT38I GRGCYC 1 cut(s) 924
ErhI CCWWGG 2 cut(s) 587, 1011
FaeI CATG 9 cut(s) 9, 325, 591, 840, 1015, 1319, 1865, 2000, 2049
FalI AAGNNNNNCTT 2 cut(s) 605, 637
FaqI GGGAC 2 cut(s) 1237, 1526
FatI CATG 9 cut(s) 5, 321, 587, 836, 1011, 1315, 1861, 1996, 2045
FauI CCCGC 2 cut(s) 457, 2012
FblI GTMKAC 2 cut(s) 54, 1139
FokI GGATG 4 cut(s) 455, 1165, 1511, 1785
FriOI GRGCYC 1 cut(s) 924
FspBI CTAG 6 cut(s) 17, 746, 789, 1490, 1545, 1805
GlaI GCGC 1 cut(s) 349
GsaI CCCAGC 1 cut(s) 825
GsuI CTGGAG 2 cut(s) 1076, 2030
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 9 cut(s) 638, 687, 722, 922, 1347, 1429, 1470, 1580, 1960
HapII CCGG 4 cut(s) 35, 85, 565, 1718
HgaI GACGC 3 cut(s) 442, 821, 1008
HhaI GCGC 1 cut(s) 350
Hin1II CATG 9 cut(s) 9, 325, 591, 840, 1015, 1319, 1865, 2000, 2049
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 1 cut(s) 1023
HindII GTYRAC 1 cut(s) 1023
HindIII AAGCTT 1 cut(s) 1331
HpaII CCGG 4 cut(s) 35, 85, 565, 1718
Hpy166II GTNNAC 8 cut(s) 14, 55, 767, 1023, 1140, 1240, 1341, 1381
Hpy188I TCNGA 8 cut(s) 142, 404, 595, 805, 1168, 1653, 1840, 1929
Hpy188III TCNNGA 9 cut(s) 284, 554, 565, 902, 957, 1153, 1289, 1786, 2009
Hpy8I GTNNAC 8 cut(s) 14, 55, 767, 1023, 1140, 1240, 1341, 1381
Hpy99I CGWCG 2 cut(s) 52, 117
HpyAV CCTTC 8 cut(s) 116, 310, 631, 712, 959, 1091, 1258, 1561
HpyCH4III ACNGT 3 cut(s) 52, 113, 1844
HpyCH4IV ACGT 3 cut(s) 57, 1136, 1753
HpyCH4V TGCA 7 cut(s) 9, 527, 630, 878, 1043, 1528, 1560
HpyF10VI GCNNNNNNNGC 8 cut(s) 785, 818, 872, 875, 928, 1040, 1330, 1733
HpyF3I CTNAG 7 cut(s) 871, 1375, 1394, 1814, 1853, 1901, 2031
HpySE526I ACGT 3 cut(s) 57, 1136, 1753
Hsp92II CATG 9 cut(s) 9, 325, 591, 840, 1015, 1319, 1865, 2000, 2049
HspAI GCGC 1 cut(s) 348
Kpn2I TCCGGA 1 cut(s) 564
Kzo9I GATC 3 cut(s) 266, 280, 1819
LmnI GCTCC 1 cut(s) 385
LweI GCATC 7 cut(s) 171, 427, 636, 887, 1187, 1377, 1515
MaeI CTAG 6 cut(s) 17, 746, 789, 1490, 1545, 1805
MaeII ACGT 3 cut(s) 57, 1136, 1753
MaeIII GTNAC 6 cut(s) 155, 697, 891, 1086, 1435, 1611
MalI GATC 3 cut(s) 268, 282, 1821
MboI GATC 3 cut(s) 266, 280, 1819
MboII GAAGA 7 cut(s) 458, 542, 727, 737, 1192, 1415, 1755
MfeI CAATTG 1 cut(s) 376
MhlI GDGCHC 4 cut(s) 72, 828, 924, 1729
MlsI TGGCCA 1 cut(s) 1580
MluCI AATT 7 cut(s) 376, 557, 664, 952, 1214, 1600, 1848
MluNI TGGCCA 1 cut(s) 1580
MlyI GAGTC 3 cut(s) 749, 1163, 1859
MmeI TCCRAC 4 cut(s) 114, 120, 1191, 1699
Mox20I TGGCCA 1 cut(s) 1580
Mph1103I ATGCAT 1 cut(s) 1530
MroI TCCGGA 1 cut(s) 564
MroXI GAANNNNTTC 1 cut(s) 1366
MscI TGGCCA 1 cut(s) 1580
MseI TTAA 1 cut(s) 1598
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 1580
MspA1I CMGCKG 3 cut(s) 875, 1922, 2019
MspI CCGG 4 cut(s) 35, 85, 565, 1718
MspR9I CCNGG 3 cut(s) 1183, 1719, 1990
MunI CAATTG 1 cut(s) 376
MvaI CCWGG 2 cut(s) 1183, 1990
MwoI GCNNNNNNNGC 8 cut(s) 785, 818, 872, 875, 928, 1040, 1330, 1733
NciI CCSGG 1 cut(s) 1719
NcoI CCATGG 2 cut(s) 587, 1011
NdeII GATC 3 cut(s) 266, 280, 1819
NlaIII CATG 9 cut(s) 9, 325, 591, 840, 1015, 1319, 1865, 2000, 2049
NlaIV GGNNCC 3 cut(s) 922, 1165, 1994
NmuCI GTSAC 4 cut(s) 891, 1086, 1435, 1611
NsiI ATGCAT 1 cut(s) 1530
NspV TTCGAA 2 cut(s) 611, 1203
OliI CACNNNNGTG 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 1366
PfeI GAWTC 8 cut(s) 760, 794, 1127, 1156, 1248, 1691, 1758, 1948
PflFI GACNNNGTC 1 cut(s) 452
PfoI TCCNGGA 1 cut(s) 1988
PleI GAGTC 3 cut(s) 749, 1163, 1859
PpsI GAGTC 3 cut(s) 749, 1163, 1859
Ppu21I YACGTR 1 cut(s) 1137
PpuMI RGGWCCY 1 cut(s) 1078
Psp5II RGGWCCY 1 cut(s) 1078
Psp6I CCWGG 2 cut(s) 1181, 1988
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 2 cut(s) 1181, 1988
PspN4I GGNNCC 3 cut(s) 922, 1165, 1994
PspOMI GGGCCC 1 cut(s) 920
PspPI GGNCC 4 cut(s) 87, 920, 921, 1078
PspPPI RGGWCCY 1 cut(s) 1078
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 456
PsyI GACNNNGTC 1 cut(s) 452
PvuII CAGCTG 2 cut(s) 875, 1922
RsaI GTAC 6 cut(s) 768, 974, 1135, 1274, 1479, 1846
RsaNI GTAC 6 cut(s) 767, 973, 1134, 1273, 1478, 1845
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 1598
Sau3AI GATC 3 cut(s) 266, 280, 1819
Sau96I GGNCC 4 cut(s) 87, 920, 921, 1078
ScaI AGTACT 1 cut(s) 1479
SchI GAGTC 3 cut(s) 749, 1163, 1859
ScrFI CCNGG 3 cut(s) 1183, 1719, 1990
SduI GDGCHC 4 cut(s) 72, 828, 924, 1729
SfaNI GCATC 7 cut(s) 171, 427, 636, 887, 1187, 1377, 1515
SfcI CTRYAG 3 cut(s) 525, 813, 1824
SfuI TTCGAA 2 cut(s) 611, 1203
SinI GGWCC 2 cut(s) 87, 1078
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 2 cut(s) 1589, 1867
SmoI CTYRAG 2 cut(s) 1589, 1867
SnaBI TACGTA 1 cut(s) 1137
SpeI ACTAGT 1 cut(s) 16
Sse9I AATT 7 cut(s) 376, 557, 664, 952, 1214, 1600, 1848
SspI AATATT 2 cut(s) 862, 1641
SspMI CTAG 6 cut(s) 17, 746, 789, 1490, 1545, 1805
StyD4I CCNGG 3 cut(s) 1181, 1717, 1988
StyI CCWWGG 2 cut(s) 587, 1011
TaaI ACNGT 3 cut(s) 52, 113, 1844
TaiI ACGT 3 cut(s) 60, 1139, 1756
TaqI TCGA 8 cut(s) 239, 580, 611, 758, 982, 1203, 1218, 1482
TaqII GACCGA 2 cut(s) 1074, 1599
TasI AATT 7 cut(s) 376, 557, 664, 952, 1214, 1600, 1848
TatI WGTACW 1 cut(s) 1477
TauI GCSGC 1 cut(s) 1963
TfiI GAWTC 8 cut(s) 760, 794, 1127, 1156, 1248, 1691, 1758, 1948
Tru1I TTAA 1 cut(s) 1598
Tru9I TTAA 1 cut(s) 1598
TscAI CASTG 2 cut(s) 358, 1041
TseFI GTSAC 4 cut(s) 891, 1086, 1435, 1611
Tsp45I GTSAC 4 cut(s) 891, 1086, 1435, 1611
TspDTI ATGAA 6 cut(s) 459, 744, 1593, 1704, 1908, 2013
TspGWI ACGGA 1 cut(s) 1507
TspRI CASTG 2 cut(s) 358, 1041
Tth111I GACNNNGTC 1 cut(s) 452
VpaK11BI GGWCC 2 cut(s) 87, 1078
XapI RAATTY 4 cut(s) 557, 952, 1214, 1600
XcmI CCANNNNNNNNNTGG 1 cut(s) 71
XmiI GTMKAC 2 cut(s) 54, 1139
XmnI GAANNNNTTC 1 cut(s) 1366
XspI CTAG 6 cut(s) 17, 746, 789, 1490, 1545, 1805
ZrmI AGTACT 1 cut(s) 1479
Zsp2I ATGCAT 1 cut(s) 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.