pycom01g16190

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0002078
Physical Location & Seq
Reverse (-)
15843315 .. 15844741
1427 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16190.1

Sequence Viewer

Length: 648 bp
ATGGCGTACAAGGTTGATCACGAATACGATTACCTCTTCAAGATTGTATTGATTGGAGATTCGGGTGTCGGAAAATCGAACATTCTTTCGAGGTTTACGAGGAATGAATTTTGTTTGGAGTCTAAGTCCACCATTGGAGTTGAATTTGCAACTAGGACATTGCAGGTAGAGGGGAAGACAGTGAAAGCACAAATATGGGACACGGCAGGTCAAGAGAGGTACCGAGCCATTACGAGTGCTTACTACAGAGGGGCTGTTGGTGCCTTATTGGTGTATGACATAACCAAGAGGCAAACATTTGACAATGCTCAGAGGTGGCTCCGCGAACTAAGAGACCACGCAGACTCTAACATTGTCATAATGATGGCCGGAAACAAATCTGACTTGAATCACCTGAGAGCAGTTCCGGCAGATGATGCTCAAATCTTGGCTGAGAAGGAAGGTCTCTCGTTCCTTGAAACTTCAGCATTGGAAGCCTTCAATGTCGAGAATGCGTTTCAGACCATTTTGTTGGACATTTATCACATAATAAGCAAGAAGGCACTAGCAGCACAGGAGGCAGTTTCTACTGGACTCCCTCAAGGAACTACCATCAATGTTGCAAATATTTCCGGTGACATGAACAAGAGAACTTGTTGCTATAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 ko04961 ko04962 ko04972 map04144 map04961 map04962 map04972
Pfam Domains
Protein Families
Ras

Protein Analysis

216

Amino Acids

24.0

Weight (kDa)

6.1

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 13 - 131 1.3e-13 ADP-ribosylation factor family
Ras PF00071 14 - 173 3.5e-61 Ras family
Roc PF08477 14 - 128 1.4e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 14 - 120 7.7e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 154, 197
Acc65I GGTACC 1 cut(s) 219
AccB1I GGYRCC 2 cut(s) 219, 260
AccII CGCG 1 cut(s) 324
AciI CCGC 1 cut(s) 322
AcoI YGGCCR 1 cut(s) 366
AcsI RAATTY 2 cut(s) 107, 143
AcuI CTGAAG 1 cut(s) 447
AfaI GTAC 2 cut(s) 8, 221
AgsI TTSAA 5 cut(s) 40, 143, 388, 458, 481
Alw26I GTCTC 2 cut(s) 327, 449
AoxI GGCC 1 cut(s) 366
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 2 cut(s) 107, 143
Asp718I GGTACC 1 cut(s) 219
AsuHPI GGTGA 2 cut(s) 383, 626
BanI GGYRCC 2 cut(s) 219, 260
BbsI GAAGAC 1 cut(s) 182
BbvI GCAGC 1 cut(s) 560
BccI CCATC 2 cut(s) 358, 599
BceAI ACGGC 1 cut(s) 219
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 2 cut(s) 327, 449
BfaI CTAG 2 cut(s) 153, 545
BfmI CTRYAG 1 cut(s) 244
BfuAI ACCTGC 2 cut(s) 154, 197
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
BmiI GGNNCC 3 cut(s) 221, 262, 320
BmsI GCATC 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 564
BsaI GGTCTC 2 cut(s) 327, 449
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 1 cut(s) 158
BseMI GCAATG 1 cut(s) 158
BseMII CTCAG 3 cut(s) 323, 386, 423
BseNI ACTGG 1 cut(s) 574
BseXI GCAGC 1 cut(s) 560
Bsh1236I CGCG 1 cut(s) 324
BshFI GGCC 1 cut(s) 368
BshNI GGYRCC 2 cut(s) 219, 260
BsiSI CCGG 3 cut(s) 369, 407, 612
BslFI GGGAC 1 cut(s) 212
BsmAI GTCTC 2 cut(s) 327, 449
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 1 cut(s) 368
Bso31I GGTCTC 2 cut(s) 327, 449
Bsp143I GATC 1 cut(s) 16
BspACI CCGC 1 cut(s) 322
BspANI GGCC 1 cut(s) 368
BspCNI CTCAG 3 cut(s) 322, 387, 424
BspFNI CGCG 1 cut(s) 324
BspLI GGNNCC 3 cut(s) 221, 262, 320
BspMI ACCTGC 2 cut(s) 154, 197
BspT107I GGYRCC 2 cut(s) 219, 260
BspTNI GGTCTC 2 cut(s) 327, 449
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 1 cut(s) 574
BssMI GATC 1 cut(s) 16
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 41
BstAPI GCANNNNNTGC 1 cut(s) 416
BstDEI CTNAG 5 cut(s) 123, 309, 329, 395, 432
BstFNI CGCG 1 cut(s) 324
BstKTI GATC 1 cut(s) 19
BstMAI GTCTC 2 cut(s) 327, 449
BstMBI GATC 1 cut(s) 16
BstMWI GCNNNNNNNGC 6 cut(s) 260, 407, 416, 473, 548, 557
BstSFI CTRYAG 1 cut(s) 244
BstUI CGCG 1 cut(s) 324
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 1 cut(s) 182
BstXI CCANNNNNNTGG 1 cut(s) 511
BsuRI GGCC 1 cut(s) 368
BtsIMutI CAGTG 1 cut(s) 186
BveI ACCTGC 2 cut(s) 154, 197
Csp6I GTAC 2 cut(s) 7, 220
CviAII CATG 1 cut(s) 619
CviJI RGCY 6 cut(s) 227, 254, 319, 368, 431, 476
CviKI_1 RGCY 6 cut(s) 227, 254, 319, 368, 431, 476
CviQI GTAC 2 cut(s) 7, 220
DdeI CTNAG 5 cut(s) 123, 309, 329, 395, 432
DpnI GATC 1 cut(s) 18
DpnII GATC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 366
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Eco31I GGTCTC 2 cut(s) 327, 449
Eco57I CTGAAG 1 cut(s) 447
FaeI CATG 1 cut(s) 622
FaiI YATR 7 cut(s) 196, 276, 281, 359, 527, 620, 642
FaqI GGGAC 1 cut(s) 212
FatI CATG 1 cut(s) 618
FbaI TGATCA 1 cut(s) 16
Fnu4HI GCNGC 1 cut(s) 549
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 2 cut(s) 153, 545
GluI GCNGC 1 cut(s) 549
HaeIII GGCC 1 cut(s) 368
HapII CCGG 3 cut(s) 369, 407, 612
Hin1II CATG 1 cut(s) 622
HinfI GANTC 5 cut(s) 59, 119, 344, 388, 573
HpaII CCGG 3 cut(s) 369, 407, 612
HphI GGTGA 2 cut(s) 383, 626
Hpy166II GTNNAC 2 cut(s) 96, 129
Hpy188I TCNGA 4 cut(s) 71, 312, 382, 501
Hpy188III TCNNGA 4 cut(s) 20, 40, 212, 487
Hpy8I GTNNAC 2 cut(s) 96, 129
HpyAV CCTTC 4 cut(s) 430, 434, 487, 532
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 3 cut(s) 149, 163, 602
HpyF10VI GCNNNNNNNGC 6 cut(s) 260, 407, 416, 473, 548, 557
HpyF3I CTNAG 5 cut(s) 123, 309, 329, 395, 432
Hsp92II CATG 1 cut(s) 622
KpnI GGTACC 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 1 cut(s) 16
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 8 cut(s) 149, 192, 382, 407, 420, 539, 555, 625
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 1 cut(s) 406
MaeI CTAG 2 cut(s) 153, 545
MaeIII GTNAC 1 cut(s) 614
MalI GATC 1 cut(s) 18
MboI GATC 1 cut(s) 16
MboII GAAGA 2 cut(s) 28, 187
MluCI AATT 2 cut(s) 107, 143
MlyI GAGTC 3 cut(s) 128, 338, 567
MmeI TCCRAC 2 cut(s) 49, 492
MslI CAYNNNNRTG 2 cut(s) 193, 362
MspI CCGG 3 cut(s) 369, 407, 612
Mva1269I GAATGC 1 cut(s) 496
MvnI CGCG 1 cut(s) 324
MwoI GCNNNNNNNGC 6 cut(s) 260, 407, 416, 473, 548, 557
NdeII GATC 1 cut(s) 16
NlaIII CATG 1 cut(s) 622
NlaIV GGNNCC 3 cut(s) 221, 262, 320
NmuCI GTSAC 1 cut(s) 614
PcsI WCGNNNNNNNCGW 1 cut(s) 95
PctI GAATGC 1 cut(s) 496
PfeI GAWTC 2 cut(s) 59, 388
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 3 cut(s) 127, 338, 567
PpsI GAGTC 3 cut(s) 127, 338, 567
PspN4I GGNNCC 3 cut(s) 221, 262, 320
RsaI GTAC 2 cut(s) 8, 221
RsaNI GTAC 2 cut(s) 7, 220
RseI CAYNNNNRTG 2 cut(s) 193, 362
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 1 cut(s) 16
SchI GAGTC 3 cut(s) 128, 338, 567
SetI ASST 9 cut(s) 15, 36, 95, 168, 211, 221, 317, 396, 445
SfaNI GCATC 1 cut(s) 406
SfcI CTRYAG 1 cut(s) 244
SmiMI CAYNNNNRTG 2 cut(s) 193, 362
SmlI CTYRAG 1 cut(s) 579
SmoI CTYRAG 1 cut(s) 579
Sse9I AATT 2 cut(s) 107, 143
SsiI CCGC 1 cut(s) 322
SspI AATATT 1 cut(s) 607
SspMI CTAG 2 cut(s) 153, 545
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 3 cut(s) 77, 89, 486
TasI AATT 2 cut(s) 107, 143
TfiI GAWTC 2 cut(s) 59, 388
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 1 cut(s) 614
TseI GCWGC 1 cut(s) 548
Tsp45I GTSAC 1 cut(s) 614
TspDTI ATGAA 2 cut(s) 120, 635
TspRI CASTG 1 cut(s) 186
XapI RAATTY 2 cut(s) 107, 143
XspI CTAG 2 cut(s) 153, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.