pycom01g17850

Appr-1'-p processing enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0004383
Physical Location & Seq
Forward (+)
17042653 .. 17044511
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGCCCAGTTACCTCTGCAGAGCATCTAGATTTCTCAGCTCAGCTCCCACGATCAGACTCGCATCATCGAACCCTGCAAGATTTCGCTGCTCTGTTAAGCTTTCGACCATGTCTAACGGAGGAGCTGTTCGCTTCCCCTTATCCGCTTCAAGCACTCTCGTAATCCAAAAGGGAGATATCACCAAGTGGTCCGTCGACGGCTCCACGGACGCCATCGTTAATCCAGCAAATGAGCGGATGCTAGGAGGTGGTGGTGCAGATGGAGCTATACATAGGGCTGCTGGCCCAGATCTTCTGCAAGCATGCTATAGTGTCCCAGAAGTCAGGCCCGGTGTTCGCTGTCCCACCGGAGAAGCAAGGATCACCCCGGGTTTTAAGTTGCCTGCATCTCATGTAATTCATACTGTTGGACCCGTGTACCATTCTGGCAGCAACCCGGAAACCTTTCTGAGTGCTGCGTACAGGAATAGCTTGACCTTAGCCAAATCAAACAACCTTCAGTATATCGCGTTTCCTGCTATTTCTTGTGGTGTGTATGGATATCCTTACGATGAAGCAGCTACAGTATCTTTATCCACAATTAAGGAGTTTGTGGATGGCCTAAAGGAGGTGCACTTTGTTCTCTTCGCAGACGATATTTATGATGTTTGGGTGAAAAAGGCCAATCAATTGCTTAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Macro

Protein Analysis

228

Amino Acids

24.27

Weight (kDa)

8.97

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 73 - 189 2e-40 Macro domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 195
AccII CGCG 1 cut(s) 509
AciI CCGC 2 cut(s) 144, 235
AclWI GGATC 1 cut(s) 368
AcuI CTGAAG 1 cut(s) 482
AcyI GRCGYC 1 cut(s) 210
AdeI CACNNNGTG 1 cut(s) 186
AfaI GTAC 2 cut(s) 419, 461
AfiI CCNNNNNNNGG 1 cut(s) 607
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 1 cut(s) 150
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 7 cut(s) 39, 44, 100, 125, 266, 471, 560
AluI AGCT 7 cut(s) 39, 44, 100, 125, 266, 471, 560
Alw21I GWGCWC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 611
AlwI GGATC 1 cut(s) 368
Ama87I CYCGRG 1 cut(s) 367
AoxI GGCC 4 cut(s) 283, 326, 598, 660
ApaLI GTGCAC 1 cut(s) 611
ApeKI GCWGC 5 cut(s) 87, 278, 429, 455, 557
Asp700I GAANNNNTTC 1 cut(s) 444
AspS9I GGNCC 4 cut(s) 189, 284, 327, 410
AsuC2I CCSGG 4 cut(s) 330, 368, 369, 437
AsuHPI GGTGA 3 cut(s) 172, 355, 664
AvaI CYCGRG 1 cut(s) 367
AvaII GGWCC 2 cut(s) 189, 410
BaeGI GKGCMC 1 cut(s) 615
BaeI ACNNNNGTAYC 2 cut(s) 401, 434
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 5 cut(s) 74, 265, 441, 442, 569
BccI CCATC 3 cut(s) 221, 254, 590
BceAI ACGGC 1 cut(s) 214
BcnI CCSGG 4 cut(s) 330, 368, 369, 437
BfaI CTAG 2 cut(s) 27, 242
BfmI CTRYAG 3 cut(s) 16, 307, 561
BfrI CTTAAG 1 cut(s) 674
BglII AGATCT 1 cut(s) 289
BisI GCNGC 5 cut(s) 88, 279, 430, 456, 558
BlpI GCTNAGC 1 cut(s) 40
BlsI GCNGC 5 cut(s) 89, 280, 431, 457, 559
Bme1390I CCNGG 4 cut(s) 330, 368, 369, 437
Bme18I GGWCC 2 cut(s) 189, 410
BmeT110I CYCGRG 1 cut(s) 367
BmgT120I GGNCC 4 cut(s) 189, 284, 327, 410
BmiI GGNNCC 2 cut(s) 202, 412
BmrFI CCNGG 4 cut(s) 330, 368, 369, 437
BmsI GCATC 4 cut(s) 32, 71, 228, 395
Bpu10I CCTNAGC 1 cut(s) 478
Bpu1102I GCTNAGC 1 cut(s) 40
BpuMI CCSGG 4 cut(s) 330, 368, 369, 437
BsaHI GRCGYC 1 cut(s) 210
BsaJI CCNNGG 3 cut(s) 204, 366, 367
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 1 cut(s) 607
Bse1I ACTGG 1 cut(s) 6
BseDI CCNNGG 3 cut(s) 204, 366, 367
BseGI GGATG 2 cut(s) 243, 601
BseLI CCNNNNNNNGG 1 cut(s) 607
BseMII CTCAG 3 cut(s) 49, 54, 440
BseNI ACTGG 1 cut(s) 6
BseRI GAGGAG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 5 cut(s) 74, 265, 441, 442, 569
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 509
BshFI GGCC 4 cut(s) 285, 328, 600, 662
BsiHKAI GWGCWC 1 cut(s) 615
BsiHKCI CYCGRG 1 cut(s) 367
BsiSI CCGG 4 cut(s) 330, 348, 368, 437
BslFI GGGAC 2 cut(s) 299, 327
BslI CCNNNNNNNGG 1 cut(s) 607
BsmFI GGGAC 2 cut(s) 299, 327
BsnI GGCC 4 cut(s) 285, 328, 600, 662
BsoBI CYCGRG 1 cut(s) 367
Bsp1286I GDGCHC 1 cut(s) 615
Bsp143I GATC 3 cut(s) 51, 289, 360
Bsp1720I GCTNAGC 1 cut(s) 40
BspACI CCGC 2 cut(s) 144, 235
BspANI GGCC 4 cut(s) 285, 328, 600, 662
BspCNI CTCAG 3 cut(s) 48, 53, 441
BspFNI CGCG 1 cut(s) 509
BspLI GGNNCC 2 cut(s) 202, 412
BspMAI CTGCAG 1 cut(s) 20
BspPI GGATC 1 cut(s) 368
BspTI CTTAAG 1 cut(s) 674
BsrBI CCGCTC 1 cut(s) 235
BsrI ACTGG 1 cut(s) 6
BssECI CCNNGG 3 cut(s) 204, 366, 367
BssMI GATC 3 cut(s) 51, 289, 360
BssNI GRCGYC 1 cut(s) 210
Bst4CI ACNGT 2 cut(s) 406, 565
Bst6I CTCTTC 1 cut(s) 629
BstACI GRCGYC 1 cut(s) 210
BstAFI CTTAAG 1 cut(s) 674
BstC8I GCNNGC 4 cut(s) 283, 300, 304, 384
BstDEI CTNAG 4 cut(s) 35, 40, 449, 478
BstDSI CCRYGG 1 cut(s) 204
BstENI CCTNNNNNAGG 1 cut(s) 605
BstF5I GGATG 2 cut(s) 243, 601
BstFNI CGCG 1 cut(s) 509
BstKTI GATC 3 cut(s) 54, 292, 363
BstMBI GATC 3 cut(s) 51, 289, 360
BstMWI GCNNNNNNNGC 2 cut(s) 263, 515
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 4 cut(s) 328, 366, 367, 435
BstSFI CTRYAG 3 cut(s) 16, 307, 561
BstSLI GKGCMC 1 cut(s) 615
BstUI CGCG 1 cut(s) 509
BstV1I GCAGC 5 cut(s) 74, 265, 441, 442, 569
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuRI GGCC 4 cut(s) 285, 328, 600, 662
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 243, 601
Cac8I GCNNGC 4 cut(s) 283, 300, 304, 384
Cfr13I GGNCC 4 cut(s) 189, 284, 327, 410
Cfr9I CCCGGG 1 cut(s) 367
CseI GACGC 1 cut(s) 218
Csp6I GTAC 2 cut(s) 418, 460
CviAII CATG 3 cut(s) 109, 303, 392
CviQI GTAC 2 cut(s) 418, 460
DdeI CTNAG 4 cut(s) 35, 40, 449, 478
DpnI GATC 3 cut(s) 53, 291, 362
DpnII GATC 3 cut(s) 51, 289, 360
DraIII CACNNNGTG 1 cut(s) 186
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Eco32I GATATC 2 cut(s) 178, 542
Eco47I GGWCC 2 cut(s) 189, 410
Eco57I CTGAAG 1 cut(s) 482
Eco88I CYCGRG 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 605
EcoRV GATATC 2 cut(s) 178, 542
FaeI CATG 3 cut(s) 112, 306, 395
FaqI GGGAC 2 cut(s) 299, 327
FatI CATG 3 cut(s) 108, 302, 391
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 88, 279, 430, 456, 558
FokI GGATG 2 cut(s) 250, 608
Fsp4HI GCNGC 5 cut(s) 88, 279, 430, 456, 558
FspBI CTAG 2 cut(s) 27, 242
GluI GCNGC 5 cut(s) 88, 279, 430, 456, 558
HaeIII GGCC 4 cut(s) 285, 328, 600, 662
HapII CCGG 4 cut(s) 330, 348, 368, 437
HgaI GACGC 1 cut(s) 218
Hin1I GRCGYC 1 cut(s) 210
Hin1II CATG 3 cut(s) 112, 306, 395
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 98
HinfI GANTC 1 cut(s) 57
HpaII CCGG 4 cut(s) 330, 348, 368, 437
HphI GGTGA 3 cut(s) 172, 355, 664
Hpy166II GTNNAC 3 cut(s) 196, 418, 613
Hpy188I TCNGA 2 cut(s) 56, 450
Hpy188III TCNNGA 1 cut(s) 27
Hpy8I GTNNAC 3 cut(s) 196, 418, 613
Hpy99I CGWCG 2 cut(s) 197, 200
HpyAV CCTTC 1 cut(s) 506
HpyCH4III ACNGT 2 cut(s) 406, 565
HpyCH4V TGCA 6 cut(s) 18, 77, 257, 298, 386, 613
HpyF10VI GCNNNNNNNGC 2 cut(s) 263, 515
HpyF3I CTNAG 4 cut(s) 35, 40, 449, 478
Hsp92I GRCGYC 1 cut(s) 210
Hsp92II CATG 3 cut(s) 112, 306, 395
Kzo9I GATC 3 cut(s) 51, 289, 360
LmnI GCTCC 4 cut(s) 49, 122, 206, 263
Lsp1109I GCAGC 5 cut(s) 74, 265, 441, 442, 569
LweI GCATC 4 cut(s) 32, 71, 228, 395
MaeI CTAG 2 cut(s) 27, 242
MaeIII GTNAC 1 cut(s) 8
MalI GATC 3 cut(s) 53, 291, 362
MbiI CCGCTC 1 cut(s) 235
MboI GATC 3 cut(s) 51, 289, 360
MboII GAAGA 2 cut(s) 284, 616
MfeI CAATTG 1 cut(s) 668
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 1 cut(s) 615
MluCI AATT 3 cut(s) 396, 579, 668
MlyI GAGTC 1 cut(s) 51
MmeI TCCRAC 1 cut(s) 388
MnlI CCTC 4 cut(s) 23, 113, 239, 601
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 6 cut(s) 96, 219, 375, 582, 675, 682
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 4 cut(s) 330, 348, 368, 437
MspR9I CCNGG 4 cut(s) 330, 368, 369, 437
MunI CAATTG 1 cut(s) 668
MvnI CGCG 1 cut(s) 509
MwoI GCNNNNNNNGC 2 cut(s) 263, 515
NciI CCSGG 4 cut(s) 330, 368, 369, 437
NdeII GATC 3 cut(s) 51, 289, 360
NlaIII CATG 3 cut(s) 112, 306, 395
NlaIV GGNNCC 2 cut(s) 202, 412
NspI RCATGY 1 cut(s) 306
PaeI GCATGC 1 cut(s) 306
PdmI GAANNNNTTC 1 cut(s) 444
PflFI GACNNNGTC 1 cut(s) 109
PkrI GCNGC 5 cut(s) 89, 280, 431, 457, 559
PleI GAGTC 1 cut(s) 51
PpsI GAGTC 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 202, 412
PspPI GGNCC 4 cut(s) 189, 284, 327, 410
PstI CTGCAG 1 cut(s) 20
PsuI RGATCY 1 cut(s) 289
PsyI GACNNNGTC 1 cut(s) 109
RsaI GTAC 2 cut(s) 419, 461
RsaNI GTAC 2 cut(s) 418, 460
SalI GTCGAC 1 cut(s) 194
SaqAI TTAA 6 cut(s) 96, 219, 375, 582, 675, 682
SatI GCNGC 5 cut(s) 88, 279, 430, 456, 558
Sau3AI GATC 3 cut(s) 51, 289, 360
Sau96I GGNCC 4 cut(s) 189, 284, 327, 410
SchI GAGTC 1 cut(s) 51
ScrFI CCNGG 4 cut(s) 330, 368, 369, 437
SduI GDGCHC 1 cut(s) 615
SfaNI GCATC 4 cut(s) 32, 71, 228, 395
SfcI CTRYAG 3 cut(s) 16, 307, 561
SgrDI CGTCGACG 1 cut(s) 194
SinI GGWCC 2 cut(s) 189, 410
SmaI CCCGGG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SphI GCATGC 1 cut(s) 306
Sse9I AATT 3 cut(s) 396, 579, 668
SsiI CCGC 2 cut(s) 144, 235
SspMI CTAG 2 cut(s) 27, 242
StyD4I CCNGG 4 cut(s) 328, 366, 367, 435
TaaI ACNGT 2 cut(s) 406, 565
TaqI TCGA 3 cut(s) 68, 104, 195
TasI AATT 3 cut(s) 396, 579, 668
Tru1I TTAA 6 cut(s) 96, 219, 375, 582, 675, 682
Tru9I TTAA 6 cut(s) 96, 219, 375, 582, 675, 682
TseI GCWGC 5 cut(s) 87, 278, 429, 455, 557
TspDTI ATGAA 2 cut(s) 389, 567
TspGWI ACGGA 3 cut(s) 132, 181, 221
TspMI CCCGGG 1 cut(s) 367
Tth111I GACNNNGTC 1 cut(s) 109
Vha464I CTTAAG 1 cut(s) 674
VneI GTGCAC 1 cut(s) 611
VpaK11BI GGWCC 2 cut(s) 189, 410
XagI CCTNNNNNAGG 1 cut(s) 605
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 1 cut(s) 306
XmaI CCCGGG 1 cut(s) 367
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 2 cut(s) 27, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.