pycom02g03620
NAC Family

proteinaceous RNase P 1, chloroplastic mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002433
Physical Location & Seq
Forward (+)
2416812 .. 2420169
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2631 bp
ATGGAGCTCTTCCAACGGCCACACATGGCCTCCTTCACCTCCAACGCACTGCAGCAAAAGCACCACCACCACCACCTCCCCTCCGCCACCCTCTGTAAGTGCCCGTCAGCTTTCCACGTCTTTAAATTCCGTTTTCCTTCTCATATTTTCACTTTCTCGCCACCCAAACAGACCCTTAAGCTTTATCCACTTCCACTAGCTAGACAAAACATCAGCCACATAGTTGCAAAGTTATCGTCGACAACGTACCACGAACCGTACACAAAATCCCAGAACTCAGGAACTGGAAGTGGTACGGAGTTGTCTTCCTTAAAGTCCACGGAACAACGGGTTTCGAGAAAATCCGAAAAGCACCATGTCGGTTCAGGAGTGGAGAAGAAGGGTGAAGATACTAGGAAAAATCTTGTCTTTAGAAAGAGGAGGGAGGGGAATGGTCCTAATGGGTCAAATGGGCATTCTTCTAGGAGGTTTAGAGATGAGAATGCGGTAAAATCTTCGAAAAGCCGAGGCGGTTTAGGGGTAAAGGAGGGGAGGAACAAGAGGGGTGGTGGTAATCAGGTGGGAGGAGAAGAGAGAAGGGAGAGAGGGTTGAAGAAGTATGATGCTGATTCGCCGGAAGTTAAGATGCGAGTAGGTTTGGATATGTGCTCGAAGAGAGGGGATGTGATGGGTGCAATTAGGTTCTATGATTTGGCTCAAAAGGAAGAGATTAAGTTGGAGCAGTACCATTATACTGTGCTGTTGTATCTCTGCTCTTCTGCAGCTGTTGGTGTTGTTCGTCCGGCGAAGAGTGGGAGTGGGAGTCGGACTTTGGATACCTTGGATTTGCACAGCGAAGAAACTAGGGTGGAATCCATGGATACATCAAATTTGGACAATGGGGACCTGGATGATACCTGTGGAAGTAACAGTTTTGATGATGATTCGGATGGTACTTCCAACGAAAAGGAGAATTTAGCATGGTTTTCTAATGGGTTTGTGAAGCGAAATTCCCGCCTTTTAGACGGACTGAACTACCCCACAAAGGGTGGAGATGATTCTTCTAATGTAAAAGATGGGAGCAGTAAGCAAGAAGATAATGGAATTCGGGTTAGTGAAGATGTGAAGAAGTATGCGCTTGAAAGGGGATTTGAGATCTATGAGAAGATGTGTTCGGATAATGTTCCGGTGAATGAGGCAGCATTGACATCTGTGGCTAGAATGGCAATGTCAATGGGTGATGGAGACATGGCATTTGATATGGTGAGGCAAATGAAGTCAATGGGAATAAATCCTAGACTCCGTTCCTACGGTCCTGCATTATCTGCTTTTTGCCATAGCGGAGATATTGATAAAGCATTTGCTGTTGAGAAACACATGCTGGAGCACGGTGTTTATCCGGAAGAGCCTGAACTTGAAGCCCTCTTAAGAGTGAGTGTGGGAGTTGGTAAAGGCGACAAGGTGTATTATATGCTGCACAAACTAAGAACAAGCGTAAGGAGGGTCTCGCCGTGTACTGCAGATTTAATTGTAAAATGGTTTCTTAGCAAGGAAGCTTCAAGAGTGGGGAGAACAAAATGGGATCGAAGATCTATAAGGGATGCAATCGAGAATGGAGGTGGGGGCTGGCATGGGCAGGGCTGGTTGGGAAAAGGGAAGTGGTCTGTGTTACGTACTACAATCGGAGATGCTGGCTTGTGCAAATGCTGTGGGGAGAAACTGGCCACAATTGACCTTGATCCCGTAGAAACAGAAAACTTTGCTGAGTCCGTTGCATCAATAGCTATAAAAAGAGAGAAAAACTCAAGCTTTCAGAAATTTCAAAAATGGCTGGACTATTATGGACCTTTTGAAGCAGTGGTGGATGGAGCTAATGTAGGCCTTTTCAGCCAGAAGAAATTTGTCCCGTCCAAGGTCAATGCTGTTGTTAATGGAATAAGGCAGAAACTTCCTTCGAAGAAATGGCCACTTATTGTTCTTCATAATAGGCGCATCACCGGAGGCAGAATGGATGAGCGAGTAAATAGGGCACTGATTGAGAAGTGGCAAAATGCCGATGCTCTATATGCGACACCTACTGGTTCAAATGATGATTGGTACTGGTTGTATGCAGCTATAAAGTTCAAGTGCTTACTTGTGACAAATGATGAGATGAGAGACCATATATTTCAACTTCTCGGAAATGATTTCTTCCCCAGGTGGAAAGAAAGGCATCAAGTGCATTTCACTTTTTCTGATGCTGGTCCAGTCTTTCACATGCCTCCTCCCTGTTCTGTTGTAATACAGGAATCAGAGGAAAGGCACTGGCACATTCCAGTTGTATCAGAACATGAGCGCGAATCAGAACGAACATGGCTATGCATTATGCGAGCTAAATCACGCACGGCAAAAAAGGATTCTGCAGCCATCCCTGAAGATGCACAACCCCCTCGTCATAACAACGGATATGCAAGGTCAGCTACCCGAACTGGTGTTGATTCACAGCCACTGAATAGTGGCAACCAAAAATATTCTAAACACAAACCTAAGGAATTTATCAAGAATCTCAAAGATATTCTGTCGGGGTCCGTGACCTCGGATCATCACTCGATTGTACCAGACATTGTAACTGCAGAGAAGATTGGTGGATGCACCATTGATTTCCAAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03013 map03013
Pfam Domains
Protein Families
PPR_long PRORP

Protein Analysis

877

Amino Acids

98.24

Weight (kDa)

9.11

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 199 - 258 7.4e-14 Pentacotripeptide-repeat region of PRORP
PPR_long PF17177 368 - 513 4.8e-52 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 394 - 507 4.1e-08 Fungal tetratrico peptide repeats
PRORP PF16953 554 - 780 1.6e-80 Protein-only RNase P
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 239
AccII CGCG 1 cut(s) 2316
AccIII TCCGGA 1 cut(s) 1378
AciI CCGC 5 cut(s) 84, 485, 510, 994, 1320
AclWI GGATC 3 cut(s) 1569, 1712, 2565
AcoI YGGCCR 3 cut(s) 17, 1701, 1943
AcsI RAATTY 8 cut(s) 125, 868, 952, 988, 1083, 1796, 1877, 2510
AcuI CTGAAG 1 cut(s) 2412
AfaI GTAC 9 cut(s) 248, 260, 295, 725, 934, 1495, 1654, 2078, 2574
AfiI CCNNNNNNNGG 3 cut(s) 1024, 1025, 1891
AflII CTTAAG 2 cut(s) 176, 1405
AgsI TTSAA 9 cut(s) 592, 1121, 1397, 1539, 1802, 1832, 2064, 2104, 2150
AjiI CACGTC 1 cut(s) 118
AjnI CCWGG 2 cut(s) 885, 2174
Alw21I GWGCWC 3 cut(s) 9, 650, 1368
Alw26I GTCTC 3 cut(s) 1218, 1489, 2130
AlwI GGATC 3 cut(s) 1569, 1712, 2565
AlwNI CAGNNNCTG 2 cut(s) 284, 2467
Aor13HI TCCGGA 1 cut(s) 1378
AoxI GGCC 5 cut(s) 17, 27, 1701, 1858, 1943
ApeKI GCWGC 6 cut(s) 52, 761, 1178, 1453, 2090, 2381
ApoI RAATTY 8 cut(s) 125, 868, 952, 988, 1083, 1796, 1877, 2510
ArsI GACNNNNNNTTYG 4 cut(s) 221, 253, 1217, 1249
AspLEI GCGC 3 cut(s) 1117, 1971, 2316
AspS9I GGNCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
AsuHPI GGTGA 6 cut(s) 28, 395, 1180, 1229, 1255, 1966
AsuII TTCGAA 2 cut(s) 497, 1934
AvaII GGWCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
AxyI CCTNAGG 1 cut(s) 2505
BaeGI GKGCMC 2 cut(s) 104, 2011
BalI TGGCCA 2 cut(s) 1703, 1945
BanII GRGCYC 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 297
Bbv12I GWGCWC 3 cut(s) 9, 650, 1368
BbvI GCAGC 6 cut(s) 64, 773, 1190, 1440, 2102, 2393
BccI CCATC 6 cut(s) 661, 923, 1049, 1214, 1838, 2393
BceAI ACGGC 3 cut(s) 32, 1474, 2379
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 2 cut(s) 887, 2176
BciVI GTATCC 2 cut(s) 808, 853
BcoDI GTCTC 3 cut(s) 1218, 1489, 2130
BfaI CTAG 7 cut(s) 197, 201, 393, 462, 843, 1197, 1275
BfmI CTRYAG 5 cut(s) 50, 759, 1497, 2379, 2589
BfrI CTTAAG 2 cut(s) 176, 1405
BfuI GTATCC 2 cut(s) 808, 853
BglII AGATCT 2 cut(s) 1134, 1568
BisI GCNGC 6 cut(s) 53, 762, 1179, 1454, 2091, 2382
BlsI GCNGC 6 cut(s) 54, 763, 1180, 1455, 2092, 2383
Bme1390I CCNGG 2 cut(s) 887, 2176
Bme18I GGWCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
BmiI GGNNCC 2 cut(s) 884, 2545
BmrFI CCNGG 2 cut(s) 887, 2176
BpiI GAAGAC 1 cut(s) 297
BplI GAGNNNNNCTC 2 cut(s) 1766, 1798
BpmI CTGGAG 1 cut(s) 1382
Bpu14I TTCGAA 2 cut(s) 497, 1934
BpuEI CTTGAG 1 cut(s) 1768
BsaAI YACGTR 1 cut(s) 1652
BsaI GGTCTC 2 cut(s) 1489, 2130
BsaJI CCNNGG 7 cut(s) 318, 505, 819, 855, 1890, 2174, 2553
BsaWI WCCGGW 3 cut(s) 1165, 1378, 1976
Bsc4I CCNNNNNNNGG 3 cut(s) 1024, 1025, 1891
Bse1I ACTGG 8 cut(s) 289, 1704, 2062, 2084, 2225, 2288, 2294, 2452
Bse21I CCTNAGG 1 cut(s) 2505
Bse3DI GCAATG 1 cut(s) 1212
BseAI TCCGGA 1 cut(s) 1378
BseBI CCWGG 2 cut(s) 887, 2176
BseDI CCNNGG 7 cut(s) 318, 505, 819, 855, 1890, 2174, 2553
BseGI GGATG 8 cut(s) 667, 895, 934, 1585, 1849, 1996, 2385, 2612
BseLI CCNNNNNNNGG 3 cut(s) 1024, 1025, 1891
BseMI GCAATG 1 cut(s) 1212
BseMII CTCAG 2 cut(s) 291, 1734
BseNI ACTGG 8 cut(s) 289, 1704, 2062, 2084, 2225, 2288, 2294, 2452
BseRI GAGGAG 3 cut(s) 433, 579, 2232
BseSI GKGCMC 2 cut(s) 104, 2011
BseXI GCAGC 6 cut(s) 64, 773, 1190, 1440, 2102, 2393
BsgI GTGCAG 1 cut(s) 1439
Bsh1236I CGCG 1 cut(s) 2316
BshFI GGCC 5 cut(s) 19, 29, 1703, 1860, 1945
BsiHKAI GWGCWC 3 cut(s) 9, 650, 1368
BsiSI CCGG 5 cut(s) 614, 782, 1166, 1379, 1977
BslFI GGGAC 2 cut(s) 896, 1868
BslI CCNNNNNNNGG 3 cut(s) 1024, 1025, 1891
BsmAI GTCTC 3 cut(s) 1218, 1489, 2130
BsmFI GGGAC 2 cut(s) 896, 1868
BsmI GAATGC 2 cut(s) 454, 487
BsnI GGCC 5 cut(s) 19, 29, 1703, 1860, 1945
Bso31I GGTCTC 2 cut(s) 1489, 2130
Bsp119I TTCGAA 2 cut(s) 497, 1934
Bsp1286I GDGCHC 5 cut(s) 9, 104, 650, 1368, 2011
Bsp13I TCCGGA 1 cut(s) 1378
Bsp143I GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
Bsp19I CCATGG 1 cut(s) 855
BspACI CCGC 5 cut(s) 84, 485, 510, 994, 1320
BspANI GGCC 5 cut(s) 19, 29, 1703, 1860, 1945
BspCNI CTCAG 2 cut(s) 290, 1735
BspEI TCCGGA 1 cut(s) 1378
BspFNI CGCG 1 cut(s) 2316
BspLI GGNNCC 2 cut(s) 884, 2545
BspMAI CTGCAG 5 cut(s) 54, 763, 1501, 2383, 2593
BspPI GGATC 3 cut(s) 1569, 1712, 2565
BspQI GCTCTTC 3 cut(s) 14, 760, 1377
BspT104I TTCGAA 2 cut(s) 497, 1934
BspTI CTTAAG 2 cut(s) 176, 1405
BspTNI GGTCTC 2 cut(s) 1489, 2130
BsrDI GCAATG 1 cut(s) 1212
BsrI ACTGG 8 cut(s) 289, 1704, 2062, 2084, 2225, 2288, 2294, 2452
BssECI CCNNGG 7 cut(s) 318, 505, 819, 855, 1890, 2174, 2553
BssMI GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
BssT1I CCWWGG 3 cut(s) 819, 855, 1890
Bst2UI CCWGG 2 cut(s) 887, 2176
Bst4CI ACNGT 5 cut(s) 258, 736, 911, 1292, 1370
Bst6I CTCTTC 7 cut(s) 14, 564, 647, 699, 760, 782, 1377
BstAFI CTTAAG 2 cut(s) 176, 1405
BstAPI GCANNNNNTGC 2 cut(s) 1304, 2197
BstBAI YACGTR 1 cut(s) 1652
BstBI TTCGAA 2 cut(s) 497, 1934
BstC8I GCNNGC 3 cut(s) 1607, 1672, 2349
BstDEI CTNAG 5 cut(s) 277, 1463, 1523, 1743, 2505
BstDSI CCRYGG 2 cut(s) 318, 855
BstF5I GGATG 8 cut(s) 667, 895, 934, 1585, 1849, 1996, 2385, 2612
BstFNI CGCG 1 cut(s) 2316
BstHHI GCGC 3 cut(s) 1117, 1971, 2316
BstKTI GATC 5 cut(s) 1137, 1564, 1571, 1720, 2560
BstMAI GTCTC 3 cut(s) 1218, 1489, 2130
BstMBI GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
BstMWI GCNNNNNNNGC 8 cut(s) 58, 1202, 1304, 1760, 1866, 2045, 2197, 2435
BstNI CCWGG 2 cut(s) 887, 2176
BstNSI RCATGY 2 cut(s) 1360, 2239
BstSCI CCNGG 2 cut(s) 885, 2174
BstSFI CTRYAG 5 cut(s) 50, 759, 1497, 2379, 2589
BstSLI GKGCMC 2 cut(s) 104, 2011
BstSNI TACGTA 1 cut(s) 1652
BstUI CGCG 1 cut(s) 2316
BstV1I GCAGC 6 cut(s) 64, 773, 1190, 1440, 2102, 2393
BstV2I GAAGAC 1 cut(s) 297
BstX2I RGATCY 2 cut(s) 1134, 1568
BstYI RGATCY 2 cut(s) 1134, 1568
Bsu36I CCTNAGG 1 cut(s) 2505
BsuI GTATCC 2 cut(s) 808, 853
BsuRI GGCC 5 cut(s) 19, 29, 1703, 1860, 1945
BtgI CCRYGG 2 cut(s) 318, 855
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 8 cut(s) 667, 895, 934, 1585, 1849, 1996, 2385, 2612
BtsI GCAGTG 2 cut(s) 47, 1842
BtsIMutI CAGTG 5 cut(s) 47, 1842, 2009, 2281, 2465
Cac8I GCNNGC 3 cut(s) 1607, 1672, 2349
CaiI CAGNNNCTG 2 cut(s) 284, 2467
CfoI GCGC 3 cut(s) 1117, 1971, 2316
Cfr13I GGNCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
Csp6I GTAC 9 cut(s) 247, 259, 294, 724, 933, 1494, 1653, 2077, 2573
CspCI CAANNNNNGTGG 4 cut(s) 526, 561, 1669, 1704
CviQI GTAC 9 cut(s) 247, 259, 294, 724, 933, 1494, 1653, 2077, 2573
DdeI CTNAG 5 cut(s) 277, 1463, 1523, 1743, 2505
DpnI GATC 5 cut(s) 1136, 1563, 1570, 1719, 2559
DpnII GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
DraI TTTAAA 1 cut(s) 124
EaeI YGGCCR 3 cut(s) 17, 1701, 1943
Eam1104I CTCTTC 7 cut(s) 14, 564, 647, 699, 760, 782, 1377
EarI CTCTTC 7 cut(s) 14, 564, 647, 699, 760, 782, 1377
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 7
Eco105I TACGTA 1 cut(s) 1652
Eco130I CCWWGG 3 cut(s) 819, 855, 1890
Eco147I AGGCCT 1 cut(s) 1860
Eco24I GRGCYC 1 cut(s) 9
Eco31I GGTCTC 2 cut(s) 1489, 2130
Eco47I GGWCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
Eco53kI GAGCTC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 2412
Eco81I CCTNAGG 1 cut(s) 2505
EcoICRI GAGCTC 1 cut(s) 7
EcoO109I RGGNCCY 1 cut(s) 883
EcoRI GAATTC 1 cut(s) 1083
EcoRII CCWGG 2 cut(s) 885, 2174
EcoT14I CCWWGG 3 cut(s) 819, 855, 1890
EcoT22I ATGCAT 1 cut(s) 2342
EcoT38I GRGCYC 1 cut(s) 9
ErhI CCWWGG 3 cut(s) 819, 855, 1890
FaqI GGGAC 2 cut(s) 896, 1868
FauI CCCGC 1 cut(s) 1001
FblI GTMKAC 1 cut(s) 239
Fnu4HI GCNGC 6 cut(s) 53, 762, 1179, 1454, 2091, 2382
FokI GGATG 8 cut(s) 674, 902, 941, 1592, 1856, 2003, 2372, 2619
FriOI GRGCYC 1 cut(s) 9
Fsp4HI GCNGC 6 cut(s) 53, 762, 1179, 1454, 2091, 2382
FspBI CTAG 7 cut(s) 197, 201, 393, 462, 843, 1197, 1275
GlaI GCGC 3 cut(s) 1116, 1970, 2315
GluI GCNGC 6 cut(s) 53, 762, 1179, 1454, 2091, 2382
GsuI CTGGAG 1 cut(s) 1382
HaeIII GGCC 5 cut(s) 19, 29, 1703, 1860, 1945
HapII CCGG 5 cut(s) 614, 782, 1166, 1379, 1977
HhaI GCGC 3 cut(s) 1117, 1971, 2316
Hin6I GCGC 3 cut(s) 1115, 1969, 2314
HinP1I GCGC 3 cut(s) 1115, 1969, 2314
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HindIII AAGCTT 3 cut(s) 179, 1533, 1786
HpaII CCGG 5 cut(s) 614, 782, 1166, 1379, 1977
HphI GGTGA 6 cut(s) 28, 395, 1180, 1229, 1255, 1966
Hpy166II GTNNAC 4 cut(s) 240, 261, 318, 1494
Hpy188III TCNNGA 7 cut(s) 279, 336, 366, 1379, 1539, 1588, 2518
Hpy8I GTNNAC 4 cut(s) 240, 261, 318, 1494
Hpy99I CGWCG 1 cut(s) 241
HpyAV CCTTC 5 cut(s) 43, 147, 373, 570, 1941
HpyCH4III ACNGT 5 cut(s) 258, 736, 911, 1292, 1370
HpyCH4IV ACGT 3 cut(s) 117, 245, 1651
HpyF10VI GCNNNNNNNGC 8 cut(s) 58, 1202, 1304, 1760, 1866, 2045, 2197, 2435
HpyF3I CTNAG 5 cut(s) 277, 1463, 1523, 1743, 2505
HpySE526I ACGT 3 cut(s) 117, 245, 1651
HspAI GCGC 3 cut(s) 1115, 1969, 2314
Kpn2I TCCGGA 1 cut(s) 1378
Kzo9I GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
LguI GCTCTTC 3 cut(s) 14, 760, 1377
LmnI GCTCC 5 cut(s) 4, 718, 1059, 1363, 1847
Lsp1109I GCAGC 6 cut(s) 64, 773, 1190, 1440, 2102, 2393
MaeI CTAG 7 cut(s) 197, 201, 393, 462, 843, 1197, 1275
MaeII ACGT 3 cut(s) 117, 245, 1651
MaeIII GTNAC 5 cut(s) 905, 1647, 2116, 2548, 2584
MalI GATC 5 cut(s) 1136, 1563, 1570, 1719, 2559
MboI GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
MfeI CAATTG 1 cut(s) 1707
MflI RGATCY 2 cut(s) 1134, 1568
MhlI GDGCHC 5 cut(s) 9, 104, 650, 1368, 2011
MlsI TGGCCA 2 cut(s) 1703, 1945
MluNI TGGCCA 2 cut(s) 1703, 1945
MlyI GAGTC 3 cut(s) 811, 1272, 1754
MmeI TCCRAC 5 cut(s) 37, 66, 696, 785, 963
Mox20I TGGCCA 2 cut(s) 1703, 1945
Mph1103I ATGCAT 1 cut(s) 2342
MroI TCCGGA 1 cut(s) 1378
MscI TGGCCA 2 cut(s) 1703, 1945
MseI TTAA 8 cut(s) 123, 177, 311, 621, 711, 1406, 1505, 1908
MslI CAYNNNNRTG 1 cut(s) 2335
Msp20I TGGCCA 2 cut(s) 1703, 1945
MspA1I CMGCKG 1 cut(s) 764
MspCI CTTAAG 2 cut(s) 176, 1405
MspI CCGG 5 cut(s) 614, 782, 1166, 1379, 1977
MspR9I CCNGG 2 cut(s) 887, 2176
MunI CAATTG 1 cut(s) 1707
Mva1269I GAATGC 2 cut(s) 454, 487
MvaI CCWGG 2 cut(s) 887, 2176
MvnI CGCG 1 cut(s) 2316
MwoI GCNNNNNNNGC 8 cut(s) 58, 1202, 1304, 1760, 1866, 2045, 2197, 2435
NcoI CCATGG 1 cut(s) 855
NdeII GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
NlaIV GGNNCC 2 cut(s) 884, 2545
NmeAIII GCCGAG 1 cut(s) 530
NmuCI GTSAC 2 cut(s) 2116, 2548
NsiI ATGCAT 1 cut(s) 2342
NspI RCATGY 2 cut(s) 1360, 2239
NspV TTCGAA 2 cut(s) 497, 1934
PceI AGGCCT 1 cut(s) 1860
PciSI GCTCTTC 3 cut(s) 14, 760, 1377
PcsI WCGNNNNNNNCGW 1 cut(s) 242
PctI GAATGC 2 cut(s) 454, 487
PfeI GAWTC 9 cut(s) 608, 851, 923, 1037, 2267, 2318, 2375, 2456, 2521
PkrI GCNGC 6 cut(s) 54, 763, 1180, 1455, 2092, 2383
PleI GAGTC 3 cut(s) 810, 1272, 1753
PpsI GAGTC 3 cut(s) 810, 1272, 1753
Ppu21I YACGTR 1 cut(s) 1652
PpuMI RGGWCCY 1 cut(s) 883
Psp124BI GAGCTC 1 cut(s) 9
Psp5II RGGWCCY 1 cut(s) 883
Psp6I CCWGG 2 cut(s) 885, 2174
PspGI CCWGG 2 cut(s) 885, 2174
PspN4I GGNNCC 2 cut(s) 884, 2545
PspPI GGNCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
PspPPI RGGWCCY 1 cut(s) 883
PstI CTGCAG 5 cut(s) 54, 763, 1501, 2383, 2593
PstNI CAGNNNCTG 2 cut(s) 284, 2467
PsuI RGATCY 2 cut(s) 1134, 1568
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 9 cut(s) 248, 260, 295, 725, 934, 1495, 1654, 2078, 2574
RsaNI GTAC 9 cut(s) 247, 259, 294, 724, 933, 1494, 1653, 2077, 2573
RseI CAYNNNNRTG 1 cut(s) 2335
SacI GAGCTC 1 cut(s) 9
SalI GTCGAC 1 cut(s) 238
SapI GCTCTTC 3 cut(s) 14, 760, 1377
SaqAI TTAA 8 cut(s) 123, 177, 311, 621, 711, 1406, 1505, 1908
SatI GCNGC 6 cut(s) 53, 762, 1179, 1454, 2091, 2382
Sau3AI GATC 5 cut(s) 1134, 1561, 1568, 1717, 2557
Sau96I GGNCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
SchI GAGTC 3 cut(s) 811, 1272, 1754
ScrFI CCNGG 2 cut(s) 887, 2176
SduI GDGCHC 5 cut(s) 9, 104, 650, 1368, 2011
SfcI CTRYAG 5 cut(s) 50, 759, 1497, 2379, 2589
SfuI TTCGAA 2 cut(s) 497, 1934
SinI GGWCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
SmiMI CAYNNNNRTG 1 cut(s) 2335
SmlI CTYRAG 3 cut(s) 176, 1405, 1783
SmoI CTYRAG 3 cut(s) 176, 1405, 1783
SnaBI TACGTA 1 cut(s) 1652
SseBI AGGCCT 1 cut(s) 1860
SsiI CCGC 5 cut(s) 84, 485, 510, 994, 1320
SspI AATATT 1 cut(s) 2489
SspMI CTAG 7 cut(s) 197, 201, 393, 462, 843, 1197, 1275
SstI GAGCTC 1 cut(s) 9
StuI AGGCCT 1 cut(s) 1860
StyD4I CCNGG 2 cut(s) 885, 2174
StyI CCWWGG 3 cut(s) 819, 855, 1890
TaaI ACNGT 5 cut(s) 258, 736, 911, 1292, 1370
TaiI ACGT 3 cut(s) 120, 248, 1654
TaqI TCGA 8 cut(s) 239, 335, 497, 650, 1564, 1587, 1934, 2567
TatI WGTACW 1 cut(s) 1493
TfiI GAWTC 9 cut(s) 608, 851, 923, 1037, 2267, 2318, 2375, 2456, 2521
Tru1I TTAA 8 cut(s) 123, 177, 311, 621, 711, 1406, 1505, 1908
Tru9I TTAA 8 cut(s) 123, 177, 311, 621, 711, 1406, 1505, 1908
TscAI CASTG 5 cut(s) 54, 1842, 2016, 2288, 2472
TseFI GTSAC 2 cut(s) 2116, 2548
TseI GCWGC 6 cut(s) 52, 761, 1178, 1453, 2090, 2381
Tsp45I GTSAC 2 cut(s) 2116, 2548
TspDTI ATGAA 2 cut(s) 1268, 1949
TspGWI ACGGA 8 cut(s) 119, 311, 335, 1020, 1271, 1738, 2436, 2536
TspRI CASTG 5 cut(s) 54, 1842, 2016, 2288, 2472
Vha464I CTTAAG 2 cut(s) 176, 1405
VpaK11BI GGWCC 6 cut(s) 434, 883, 1292, 1823, 2222, 2544
XapI RAATTY 8 cut(s) 125, 868, 952, 988, 1083, 1796, 1877, 2510
XceI RCATGY 2 cut(s) 1360, 2239
XmiI GTMKAC 1 cut(s) 239
XspI CTAG 7 cut(s) 197, 201, 393, 462, 843, 1197, 1275
Zsp2I ATGCAT 1 cut(s) 2342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.