pycom02g18640

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005196
Physical Location & Seq
Reverse (-)
16792847 .. 16795274
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1617 bp
ATGGCAGCAGCAAAGATGATAGTATCAGCACTGCTGGGAGGCTTTGTGTTATTCGTATTATATGTCTATGAATCTTTGGTCTTAAAGCCAAAGAGGCTAAGATCAAAGCTTCAAAAGCAAGGGATCAGAGGGCCTCCCCCTTCATCAATTCTCTTAGGCAATATCCCGGACATGAAGAGCATCAAACTCAAGGTGATGAGCGAGAAGTCAACAGCTACAGAAACAAGTAGTACCAGTGTCTCAAAAGACCAGCATGTTTCGATTAATCATGATTGGCCTTCAACCCTTTTCCCTCATTTAGCGCAATGGCGAAACGACTATGGGCCAAACTTCACATATTTAACTGGAAGCATACAGCAATTAAGCATAACAGATTTAGAAATGGTAAAGGAAGTTTGTTTGTGTAGATCTTTGAACTTAGGGAGGCCTACTTTCGTATCTAAGGATCGTAAGCCACTGTTCGGCCAAGGAATTATAGCGTCAAATGGTCCAATTTGGTTGCACCAAAAAAAAATTATTGGTCCAGAGTTCTACTTTGAAAGGGTTAAGGGTATGGTGGATTTGATGGTGGACTCCACAACCATCATGTTGAGATCTTGGGAGAGTAAAATCGAAAACGAAGGAGGAAGTGCAGCCTTAGAAGTTGATAAGGATTTGAGAAGCTTGTCGGCCGATATTATATCAAGAACGTCGTTTGGAAGTAATTATTCACAAGGGAAAGAAATATTTTTAAAGCTCAGAACTCTTCAGAAGATCATGTCCCAGGGAAACACTGGTGTACCGGGTGCGAGGTTTGTTAAATGTATGAATGCCAGACACTTACCAACGAAAAACAACAGACTGATATGGAGATTAGAGAAAGAGATCCGTGCTATGATTTTAAGGGTTGCGAAGGAACGCAGTACTGAGGCTACTTGTGAGAAGGATCTTCTGCAAATAATACTCGAGGGAGCCAAAAATTATGAAGACGCTCCTAGCCTATTTTCAGGTATATCCCAGGATAGTTTTATAGTGGATAATTGCAAGAGTATATACTTTGCTGGCCACGAAACCACTGCCACAGCAGCGTCTTGGAGCTTGATGTTATTAGCTGCACATCAAGACTGGCAAACTTGTGCCCGTGCTGAGGTGCTTGAATTTTGTAGGGATGGCATTCCAGATGCTGATTTGCTTCGAAACATGAAAATATTGAATATGGTGATTCAAGAGACATTGCGCCTGTACTCACCATCAACGGTTGTTGCAAGAGAAGCCTTGGAAGATATTGAGCTGAAAGGCATTCTAATTCCCAAAGGAACGAACATTCAGATTCCAATTCCTATAGTGCATCGACTTCCTGATATCTGGGGTCCCGATGCCCATGAATTCAATCCGAAAAGATTCGAGCATGGAATTGCTGGGGCGTGCAAGTTTCCTCAGGCTTATATGCCATTTAGAGTTGGTGCTCGTATCTGTACTGGCCAACACTTAGCCATGACAGAGTTGAAGGTGATTCTGTCACTTATTCTGTCCAAGTTTTCCTTCTCACTATCACCAGAATACCAACACTCCCCGGCTTATACGTTGGTTATTATGGAACCCGAACATGGAGTAAGTCTCCATATGAGGAGAGTGTGA

Protein Analysis

539

Amino Acids

60.38

Weight (kDa)

9.08

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 99 - 525 5.9e-74 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 131, 453, 859, 933
AcoI YGGCCR 4 cut(s) 463, 669, 1042, 1459
AcsI RAATTY 2 cut(s) 1136, 1364
AcuI CTGAAG 1 cut(s) 731
AfaI GTAC 5 cut(s) 232, 780, 904, 1223, 1456
AfiI CCNNNNNNNGG 3 cut(s) 461, 1126, 1586
AgsI TTSAA 9 cut(s) 113, 282, 415, 539, 1136, 1192, 1205, 1369, 1486
AjnI CCWGG 2 cut(s) 762, 996
AjuI GAANNNNNNNTTGG 2 cut(s) 1505, 1537
AluBI AGCT 7 cut(s) 109, 215, 663, 736, 1077, 1091, 1270
AluI AGCT 7 cut(s) 109, 215, 663, 736, 1077, 1091, 1270
Alw21I GWGCWC 1 cut(s) 1447
Alw26I GTCTC 3 cut(s) 244, 1202, 1601
AlwI GGATC 4 cut(s) 131, 453, 859, 933
AlwNI CAGNNNCTG 1 cut(s) 1163
Ama87I CYCGRG 1 cut(s) 944
AoxI GGCC 8 cut(s) 131, 275, 323, 425, 463, 669, 1042, 1459
ApeKI GCWGC 5 cut(s) 5, 8, 632, 1064, 1091
ApoI RAATTY 2 cut(s) 1136, 1364
AseI ATTAAT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 1379
AspLEI GCGC 2 cut(s) 304, 1218
AspS9I GGNCC 5 cut(s) 131, 323, 488, 521, 1349
AsuC2I CCSGG 3 cut(s) 167, 783, 1553
AsuHPI GGTGA 5 cut(s) 205, 1210, 1218, 1501, 1524
AsuII TTCGAA 1 cut(s) 1174
AvaI CYCGRG 1 cut(s) 944
AvaII GGWCC 3 cut(s) 488, 521, 1349
AxyI CCTNAGG 1 cut(s) 1416
BaeGI GKGCMC 1 cut(s) 1120
BaeI ACNNNNGTAYC 4 cut(s) 420, 453, 762, 795
BalI TGGCCA 2 cut(s) 1044, 1461
BbsI GAAGAC 1 cut(s) 972
Bbv12I GWGCWC 1 cut(s) 1447
BbvCI CCTCAGC 1 cut(s) 1125
BbvI GCAGC 5 cut(s) 17, 20, 644, 1076, 1078
BccI CCATC 4 cut(s) 559, 590, 1142, 1237
BcgI CGANNNNNNTGC 2 cut(s) 1037, 1071
BciT130I CCWGG 2 cut(s) 764, 998
BcnI CCSGG 3 cut(s) 167, 783, 1553
BcoDI GTCTC 3 cut(s) 244, 1202, 1601
BfaI CTAG 1 cut(s) 975
BfmI CTRYAG 2 cut(s) 216, 1320
BglI GCCNNNNNGGC 1 cut(s) 94
BglII AGATCT 2 cut(s) 407, 593
BisI GCNGC 5 cut(s) 6, 9, 633, 1065, 1092
BlsI GCNGC 5 cut(s) 7, 10, 634, 1066, 1093
BmcAI AGTACT 1 cut(s) 904
Bme1390I CCNGG 5 cut(s) 167, 764, 783, 998, 1553
Bme18I GGWCC 3 cut(s) 488, 521, 1349
BmeT110I CYCGRG 1 cut(s) 944
BmgT120I GGNCC 5 cut(s) 131, 323, 488, 521, 1349
BmiI GGNNCC 4 cut(s) 952, 1350, 1351, 1578
BmrFI CCNGG 5 cut(s) 167, 764, 783, 998, 1553
BmsI GCATC 4 cut(s) 189, 1150, 1336, 1345
BpiI GAAGAC 1 cut(s) 972
BplI GAGNNNNNCTC 2 cut(s) 1581, 1613
Bpu10I CCTNAGC 1 cut(s) 1125
Bpu14I TTCGAA 1 cut(s) 1174
BpuEI CTTGAG 1 cut(s) 173
BpuMI CCSGG 3 cut(s) 167, 783, 1553
BsaJI CCNNGG 6 cut(s) 466, 762, 763, 996, 1254, 1551
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 1532, 1562
Bsc4I CCNNNNNNNGG 3 cut(s) 461, 1126, 1586
Bse1I ACTGG 5 cut(s) 234, 349, 778, 1109, 1462
Bse21I CCTNAGG 1 cut(s) 1416
Bse3DI GCAATG 2 cut(s) 311, 1211
BseBI CCWGG 2 cut(s) 764, 998
BseDI CCNNGG 6 cut(s) 466, 762, 763, 996, 1254, 1551
BseGI GGATG 1 cut(s) 1153
BseLI CCNNNNNNNGG 3 cut(s) 461, 1126, 1586
BseMI GCAATG 2 cut(s) 311, 1211
BseMII CTCAG 4 cut(s) 751, 897, 1116, 1430
BseNI ACTGG 5 cut(s) 234, 349, 778, 1109, 1462
BseSI GKGCMC 1 cut(s) 1120
BseX3I CGGCCG 1 cut(s) 669
BseXI GCAGC 5 cut(s) 17, 20, 644, 1076, 1078
BseYI CCCAGC 2 cut(s) 34, 1397
BsgI GTGCAG 2 cut(s) 651, 1077
Bsh1285I CGRYCG 1 cut(s) 672
BshFI GGCC 8 cut(s) 133, 277, 325, 427, 465, 671, 1044, 1461
BsiEI CGRYCG 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 1447
BsiHKCI CYCGRG 1 cut(s) 944
BsiSI CCGG 3 cut(s) 167, 782, 1553
BslFI GGGAC 2 cut(s) 745, 1335
BslI CCNNNNNNNGG 3 cut(s) 461, 1126, 1586
BsmAI GTCTC 3 cut(s) 244, 1202, 1601
BsmFI GGGAC 2 cut(s) 745, 1335
BsmI GAATGC 3 cut(s) 814, 1152, 1278
BsnI GGCC 8 cut(s) 133, 277, 325, 427, 465, 671, 1044, 1461
BsoBI CYCGRG 1 cut(s) 944
Bsp119I TTCGAA 1 cut(s) 1174
Bsp1286I GDGCHC 2 cut(s) 1120, 1447
Bsp143I GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
BspANI GGCC 8 cut(s) 133, 277, 325, 427, 465, 671, 1044, 1461
BspCNI CTCAG 4 cut(s) 750, 898, 1117, 1429
BspHI TCATGA 1 cut(s) 268
BspLI GGNNCC 4 cut(s) 952, 1350, 1351, 1578
BspPI GGATC 4 cut(s) 131, 453, 859, 933
BspQI GCTCTTC 1 cut(s) 170
BspT104I TTCGAA 1 cut(s) 1174
BsrDI GCAATG 2 cut(s) 311, 1211
BsrI ACTGG 5 cut(s) 234, 349, 778, 1109, 1462
BssECI CCNNGG 6 cut(s) 466, 762, 763, 996, 1254, 1551
BssMI GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
BssT1I CCWWGG 2 cut(s) 466, 1254
Bst2UI CCWGG 2 cut(s) 764, 998
Bst4CI ACNGT 2 cut(s) 459, 1237
Bst6I CTCTTC 2 cut(s) 170, 750
BstBI TTCGAA 1 cut(s) 1174
BstC8I GCNNGC 2 cut(s) 1042, 1405
BstF5I GGATG 1 cut(s) 1153
BstHHI GCGC 2 cut(s) 304, 1218
BstKTI GATC 8 cut(s) 104, 126, 410, 448, 596, 756, 867, 928
BstMAI GTCTC 3 cut(s) 244, 1202, 1601
BstMBI GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 4 cut(s) 94, 115, 1064, 1250
BstNI CCWGG 2 cut(s) 764, 998
BstNSI RCATGY 1 cut(s) 257
BstSCI CCNGG 5 cut(s) 165, 762, 781, 996, 1551
BstSFI CTRYAG 2 cut(s) 216, 1320
BstSLI GKGCMC 1 cut(s) 1120
BstV1I GCAGC 5 cut(s) 17, 20, 644, 1076, 1078
BstV2I GAAGAC 1 cut(s) 972
BstX2I RGATCY 4 cut(s) 407, 593, 864, 925
BstYI RGATCY 4 cut(s) 407, 593, 864, 925
BstZI CGGCCG 1 cut(s) 669
Bsu36I CCTNAGG 1 cut(s) 1416
BsuRI GGCC 8 cut(s) 133, 277, 325, 427, 465, 671, 1044, 1461
BtsCI GGATG 1 cut(s) 1153
BtsI GCAGTG 2 cut(s) 29, 1053
BtsIMutI CAGTG 5 cut(s) 29, 241, 455, 771, 1053
Cac8I GCNNGC 2 cut(s) 1042, 1405
CaiI CAGNNNCTG 1 cut(s) 1163
CciI TCATGA 1 cut(s) 268
CfoI GCGC 2 cut(s) 304, 1218
Cfr13I GGNCC 5 cut(s) 131, 323, 488, 521, 1349
CseI GACGC 3 cut(s) 468, 977, 1056
Csp6I GTAC 5 cut(s) 231, 779, 903, 1222, 1455
CviQI GTAC 5 cut(s) 231, 779, 903, 1222, 1455
DpnI GATC 8 cut(s) 103, 125, 409, 447, 595, 755, 866, 927
DpnII GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
DraI TTTAAA 1 cut(s) 732
EaeI YGGCCR 4 cut(s) 463, 669, 1042, 1459
EagI CGGCCG 1 cut(s) 669
Eam1104I CTCTTC 2 cut(s) 170, 750
EarI CTCTTC 2 cut(s) 170, 750
EclXI CGGCCG 1 cut(s) 669
Eco130I CCWWGG 2 cut(s) 466, 1254
Eco147I AGGCCT 1 cut(s) 427
Eco32I GATATC 1 cut(s) 1342
Eco47I GGWCC 3 cut(s) 488, 521, 1349
Eco52I CGGCCG 1 cut(s) 669
Eco57I CTGAAG 1 cut(s) 731
Eco81I CCTNAGG 1 cut(s) 1416
Eco88I CYCGRG 1 cut(s) 944
EcoO109I RGGNCCY 2 cut(s) 131, 1349
EcoRI GAATTC 1 cut(s) 1364
EcoRII CCWGG 2 cut(s) 762, 996
EcoRV GATATC 1 cut(s) 1342
EcoT14I CCWWGG 2 cut(s) 466, 1254
ErhI CCWWGG 2 cut(s) 466, 1254
FalI AAGNNNNNCTT 2 cut(s) 1505, 1537
FaqI GGGAC 2 cut(s) 745, 1335
FauNDI CATATG 1 cut(s) 1602
Fnu4HI GCNGC 5 cut(s) 6, 9, 633, 1065, 1092
FokI GGATG 1 cut(s) 1160
Fsp4HI GCNGC 5 cut(s) 6, 9, 633, 1065, 1092
FspBI CTAG 1 cut(s) 975
GlaI GCGC 2 cut(s) 303, 1217
GluI GCNGC 5 cut(s) 6, 9, 633, 1065, 1092
GsaI CCCAGC 2 cut(s) 38, 1401
HaeIII GGCC 8 cut(s) 133, 277, 325, 427, 465, 671, 1044, 1461
HapII CCGG 3 cut(s) 167, 782, 1553
HgaI GACGC 3 cut(s) 468, 977, 1056
HhaI GCGC 2 cut(s) 304, 1218
Hin6I GCGC 2 cut(s) 302, 1216
HinP1I GCGC 2 cut(s) 302, 1216
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HindIII AAGCTT 2 cut(s) 107, 661
HinfI GANTC 6 cut(s) 71, 572, 1201, 1309, 1380, 1492
HpaII CCGG 3 cut(s) 167, 782, 1553
HphI GGTGA 5 cut(s) 205, 1210, 1218, 1501, 1524
Hpy166II GTNNAC 3 cut(s) 210, 571, 779
Hpy188I TCNGA 5 cut(s) 128, 740, 750, 1308, 1374
Hpy188III TCNNGA 8 cut(s) 269, 524, 684, 1100, 1157, 1205, 1337, 1352
Hpy8I GTNNAC 3 cut(s) 210, 571, 779
Hpy99I CGWCG 1 cut(s) 694
HpyAV CCTTC 7 cut(s) 150, 288, 614, 886, 916, 1480, 1531
HpyCH4III ACNGT 2 cut(s) 459, 1237
HpyCH4IV ACGT 2 cut(s) 689, 1562
HpyCH4V TGCA 8 cut(s) 502, 632, 934, 1023, 1094, 1244, 1327, 1407
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 115, 1064, 1250
HpySE526I ACGT 2 cut(s) 689, 1562
HspAI GCGC 2 cut(s) 302, 1216
KflI GGGWCCC 1 cut(s) 1349
Kzo9I GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
LguI GCTCTTC 1 cut(s) 170
LmnI GCTCC 3 cut(s) 950, 976, 1074
Lsp1109I GCAGC 5 cut(s) 17, 20, 644, 1076, 1078
LweI GCATC 4 cut(s) 189, 1150, 1336, 1345
MaeI CTAG 1 cut(s) 975
MaeII ACGT 2 cut(s) 689, 1562
MaeIII GTNAC 1 cut(s) 1497
MalI GATC 8 cut(s) 103, 125, 409, 447, 595, 755, 866, 927
MboI GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
MboII GAAGA 6 cut(s) 187, 737, 763, 920, 977, 1271
MflI RGATCY 4 cut(s) 407, 593, 864, 925
MhlI GDGCHC 2 cut(s) 1120, 1447
MlsI TGGCCA 2 cut(s) 1044, 1461
MluNI TGGCCA 2 cut(s) 1044, 1461
MlyI GAGTC 1 cut(s) 566
Mox20I TGGCCA 2 cut(s) 1044, 1461
MroXI GAANNNNTTC 1 cut(s) 1379
MscI TGGCCA 2 cut(s) 1044, 1461
MseI TTAA 8 cut(s) 83, 264, 341, 362, 546, 731, 798, 881
Msp20I TGGCCA 2 cut(s) 1044, 1461
MspI CCGG 3 cut(s) 167, 782, 1553
MspR9I CCNGG 5 cut(s) 167, 764, 783, 998, 1553
Mva1269I GAATGC 3 cut(s) 814, 1152, 1278
MvaI CCWGG 2 cut(s) 764, 998
MwoI GCNNNNNNNGC 4 cut(s) 94, 115, 1064, 1250
NciI CCSGG 3 cut(s) 167, 783, 1553
NdeI CATATG 1 cut(s) 1602
NdeII GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
NlaIV GGNNCC 4 cut(s) 952, 1350, 1351, 1578
NmuCI GTSAC 1 cut(s) 1497
NspI RCATGY 1 cut(s) 257
NspV TTCGAA 1 cut(s) 1174
PaeR7I CTCGAG 1 cut(s) 944
PagI TCATGA 1 cut(s) 268
PasI CCCWGGG 1 cut(s) 763
PceI AGGCCT 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 170
PctI GAATGC 3 cut(s) 814, 1152, 1278
PdmI GAANNNNTTC 1 cut(s) 1379
PfeI GAWTC 5 cut(s) 71, 1201, 1309, 1380, 1492
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 5 cut(s) 7, 10, 634, 1066, 1093
PleI GAGTC 1 cut(s) 566
PpsI GAGTC 1 cut(s) 566
PpuMI RGGWCCY 1 cut(s) 1349
PshBI ATTAAT 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 1349
Psp6I CCWGG 2 cut(s) 762, 996
PspFI CCCAGC 2 cut(s) 34, 1397
PspGI CCWGG 2 cut(s) 762, 996
PspN4I GGNNCC 4 cut(s) 952, 1350, 1351, 1578
PspPI GGNCC 5 cut(s) 131, 323, 488, 521, 1349
PspPPI RGGWCCY 1 cut(s) 1349
PspXI VCTCGAGB 1 cut(s) 944
PstNI CAGNNNCTG 1 cut(s) 1163
PsuI RGATCY 4 cut(s) 407, 593, 864, 925
RsaI GTAC 5 cut(s) 232, 780, 904, 1223, 1456
RsaNI GTAC 5 cut(s) 231, 779, 903, 1222, 1455
SapI GCTCTTC 1 cut(s) 170
SaqAI TTAA 8 cut(s) 83, 264, 341, 362, 546, 731, 798, 881
SatI GCNGC 5 cut(s) 6, 9, 633, 1065, 1092
Sau3AI GATC 8 cut(s) 101, 123, 407, 445, 593, 753, 864, 925
Sau96I GGNCC 5 cut(s) 131, 323, 488, 521, 1349
ScaI AGTACT 1 cut(s) 904
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 5 cut(s) 167, 764, 783, 998, 1553
SduI GDGCHC 2 cut(s) 1120, 1447
SfaNI GCATC 4 cut(s) 189, 1150, 1336, 1345
SfcI CTRYAG 2 cut(s) 216, 1320
Sfr274I CTCGAG 1 cut(s) 944
SfuI TTCGAA 1 cut(s) 1174
SinI GGWCC 3 cut(s) 488, 521, 1349
SlaI CTCGAG 1 cut(s) 944
SmlI CTYRAG 2 cut(s) 188, 944
SmoI CTYRAG 2 cut(s) 188, 944
SseBI AGGCCT 1 cut(s) 427
SspI AATATT 2 cut(s) 726, 1188
SspMI CTAG 1 cut(s) 975
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 5 cut(s) 165, 762, 781, 996, 1551
StyI CCWWGG 2 cut(s) 466, 1254
TaaI ACNGT 2 cut(s) 459, 1237
TaiI ACGT 2 cut(s) 692, 1565
TaqI TCGA 6 cut(s) 260, 612, 945, 1174, 1330, 1383
TatI WGTACW 3 cut(s) 902, 1221, 1454
TfiI GAWTC 5 cut(s) 71, 1201, 1309, 1380, 1492
Tru1I TTAA 8 cut(s) 83, 264, 341, 362, 546, 731, 798, 881
Tru9I TTAA 8 cut(s) 83, 264, 341, 362, 546, 731, 798, 881
TscAI CASTG 5 cut(s) 36, 241, 462, 778, 1060
TseFI GTSAC 1 cut(s) 1497
TseI GCWGC 5 cut(s) 5, 8, 632, 1064, 1091
Tsp45I GTSAC 1 cut(s) 1497
TspDTI ATGAA 7 cut(s) 84, 132, 188, 821, 978, 1196, 1377
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 5 cut(s) 36, 241, 462, 778, 1060
VpaK11BI GGWCC 3 cut(s) 488, 521, 1349
VspI ATTAAT 1 cut(s) 264
XapI RAATTY 2 cut(s) 1136, 1364
XceI RCATGY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 770
XhoI CTCGAG 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 1379
XspI CTAG 1 cut(s) 975
ZrmI AGTACT 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.