pycom02g24330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0009711
Physical Location & Seq
Reverse (-)
22464508 .. 22468993
4486 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1293 bp
ATGACTTGGTTGGGTGCTTCCTCTTCTTCTTCCCCATGGATTCTCAGGAACTCGCTTCTCACAACATCCACAACCTCTTCATCTTCTTCTTCCTCTTCAAAACTCTCGAAATACCGATCATGCCCTCGCCCTCTTCGCCTCTCGAGCCGCGCCCAAGCCGACGTTGGCAGTGACTTCAATTTCGTGCTCCACGATGCTCTCGATTCTTCCGGAACCCTCACCTCCCTCGCCAGAGAGGCCAGGGAGGGGTTCTGCTCACAAATTCGCCATCTGTCTGATATAGAGAGGGAAACCAGCATTACCATCAATAGATGTGTTGATTTGGGGAAAACAGCTCTTTACATTGCAGCAGAAGATGACTCCCTTGTGTCACATTCTTCAGTTCCGCTTCCGGTGGATGCTTTTATTAGTAGGTTTGGTGATCTCTCCATGGACTATTGTTCTCACCACAACTCTTCATTTAGATCCTCGCCTGAGAAATTGTTGGAGAGTTTGGAAAACTATTTGTACGTAAATAAGGGTTTTCGAAGGACGATTGCAAGGTATCACTCTGAATCACGGGCTCTATATCTCCACTCAGTTTTGACGCATCGTTCAGGATCACCTGCAATGCTTTCACTCATATACTCAGAAATTTTGAAAATGCTTCGCCTATGGGGTTTTTTGGATTTCGATGTAGAGATATTCTTTCCTCATGATCTTCATAGTCTTCCCCGAGGTTATCATAAACAGAAAAGCAAGGAATCTGATAATGTCCACATAATGACTTCGCAAGCCCTTTTAGTGGAGATTTTAAGAAATTTGAAGGACGCATTCTGGCCGTTTCAGCATGATCAACCAGGGAATTTGTTCTTGAGGGCAGCACGTGCTGCTAACTTTATCGATAGATCAAACATAAATGAAGACAGTGGCTTTCAGCTTGCATCTGCGAAGGCTGCTCAACATAGACTAGATCGTGGTGTTTGGACTAGTGTACATTTTGGGGATATGAGGCGTGCCCTATCTGCTTGTGAACGTCTTATTCTCCTGGAAGCTGATCCGAAGGAATTAAGAGATTACAGCATTCTTCTCTACCATTGTGGATTTTACGAGGAATCACTTGAATATCTAAAGTTATATCAGGACAAAAAGGGTCGTCCACTACGGAGGCGACCTTCTGATTCGTTAACCGTTTTGGAGGAAGATGCCGCAGAGAAATTGATGATACGCCTTAACCTAATTCTGATGGAGGATGGTTGGAGCAGGCCGTCATATATTAGAAACTTTCTTGGTAACAACTCTGAACCATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transglut_core2

Protein Analysis

431

Amino Acids

49.03

Weight (kDa)

7.28

Isoelectric Point (pI)

58.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transglut_core2 PF13369 156 - 217 7.1e-09 Transglutaminase-like superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 613
AccII CGCG 1 cut(s) 150
AccIII TCCGGA 1 cut(s) 209
AciI CCGC 3 cut(s) 148, 386, 1188
AclWI GGATC 3 cut(s) 459, 607, 1031
AcoI YGGCCR 1 cut(s) 818
AcsI RAATTY 4 cut(s) 261, 633, 799, 844
AcuI CTGAAG 1 cut(s) 363
AcvI CACGTG 1 cut(s) 866
AfaI GTAC 2 cut(s) 509, 975
AfiI CCNNNNNNNGG 2 cut(s) 246, 784
AgsI TTSAA 5 cut(s) 99, 178, 640, 805, 1103
AhlI ACTAGT 1 cut(s) 968
AjnI CCWGG 3 cut(s) 239, 838, 1026
AluBI AGCT 3 cut(s) 335, 919, 1034
AluI AGCT 3 cut(s) 335, 919, 1034
Alw21I GWGCWC 1 cut(s) 189
AlwI GGATC 3 cut(s) 459, 607, 1031
Ama87I CYCGRG 2 cut(s) 142, 714
Aor13HI TCCGGA 1 cut(s) 209
AoxI GGCC 3 cut(s) 237, 818, 1244
ApeKI GCWGC 4 cut(s) 347, 860, 869, 935
ApoI RAATTY 4 cut(s) 261, 633, 799, 844
ArsI GACNNNNNNTTYG 2 cut(s) 164, 196
Asp700I GAANNNNTTC 1 cut(s) 848
AspLEI GCGC 1 cut(s) 152
AsuHPI GGTGA 4 cut(s) 211, 431, 437, 594
AsuII TTCGAA 1 cut(s) 526
AvaI CYCGRG 2 cut(s) 142, 714
BaeGI GKGCMC 1 cut(s) 1000
BanII GRGCYC 1 cut(s) 565
BbrPI CACGTG 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 701, 909
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 4 cut(s) 359, 856, 872, 922
BccI CCATC 4 cut(s) 276, 311, 1219, 1226
BceAI ACGGC 2 cut(s) 805, 1231
BciT130I CCWGG 3 cut(s) 241, 840, 1028
BclI TGATCA 1 cut(s) 832
BcuI ACTAGT 1 cut(s) 968
BfaI CTAG 2 cut(s) 950, 969
BfuAI ACCTGC 1 cut(s) 613
BglI GCCNNNNNGGC 1 cut(s) 236
BisI GCNGC 6 cut(s) 148, 348, 861, 870, 936, 1188
BlsI GCNGC 6 cut(s) 149, 349, 862, 871, 937, 1189
Bme1390I CCNGG 3 cut(s) 241, 840, 1028
BmeT110I CYCGRG 2 cut(s) 142, 714
BmiI GGNNCC 1 cut(s) 214
BmrFI CCNGG 3 cut(s) 241, 840, 1028
BmsI GCATC 5 cut(s) 184, 388, 598, 932, 1174
BpiI GAAGAC 2 cut(s) 701, 909
Bpu14I TTCGAA 1 cut(s) 526
BpuEI CTTGAG 1 cut(s) 874
Bsa29I ATCGAT 1 cut(s) 882
BsaAI YACGTR 2 cut(s) 511, 866
BsaJI CCNNGG 6 cut(s) 35, 240, 429, 715, 839, 1286
BsaWI WCCGGW 2 cut(s) 209, 391
Bsc4I CCNNNNNNNGG 2 cut(s) 246, 784
Bse3DI GCAATG 2 cut(s) 342, 615
BseAI TCCGGA 1 cut(s) 209
BseBI CCWGG 3 cut(s) 241, 840, 1028
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 6 cut(s) 35, 240, 429, 715, 839, 1286
BseGI GGATG 3 cut(s) 65, 403, 1237
BseLI CCNNNNNNNGG 2 cut(s) 246, 784
BseMI GCAATG 2 cut(s) 342, 615
BseMII CTCAG 4 cut(s) 58, 465, 591, 642
BseSI GKGCMC 1 cut(s) 1000
BseXI GCAGC 4 cut(s) 359, 856, 872, 922
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 3 cut(s) 239, 820, 1246
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 1 cut(s) 189
BsiHKCI CYCGRG 2 cut(s) 142, 714
BsiSI CCGG 2 cut(s) 210, 392
BslI CCNNNNNNNGG 2 cut(s) 246, 784
BsmI GAATGC 2 cut(s) 812, 1062
BsnI GGCC 3 cut(s) 239, 820, 1246
BsoBI CYCGRG 2 cut(s) 142, 714
Bsp119I TTCGAA 1 cut(s) 526
Bsp1286I GDGCHC 3 cut(s) 189, 565, 1000
Bsp13I TCCGGA 1 cut(s) 209
Bsp1407I TGTACA 1 cut(s) 973
Bsp143I GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
Bsp19I CCATGG 3 cut(s) 35, 429, 1286
BspACI CCGC 3 cut(s) 148, 386, 1188
BspANI GGCC 3 cut(s) 239, 820, 1246
BspCNI CTCAG 4 cut(s) 57, 466, 590, 641
BspDI ATCGAT 1 cut(s) 882
BspEI TCCGGA 1 cut(s) 209
BspFNI CGCG 1 cut(s) 150
BspHI TCATGA 1 cut(s) 694
BspLI GGNNCC 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 3 cut(s) 459, 607, 1031
BspT104I TTCGAA 1 cut(s) 526
BsrDI GCAATG 2 cut(s) 342, 615
BsrGI TGTACA 1 cut(s) 973
BssECI CCNNGG 6 cut(s) 35, 240, 429, 715, 839, 1286
BssMI GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
BssT1I CCWWGG 3 cut(s) 35, 429, 1286
Bst2UI CCWGG 3 cut(s) 241, 840, 1028
Bst4CI ACNGT 2 cut(s) 908, 1171
Bst6I CTCTTC 5 cut(s) 28, 82, 100, 138, 460
BstAPI GCANNNNNTGC 2 cut(s) 866, 869
BstAUI TGTACA 1 cut(s) 973
BstBAI YACGTR 2 cut(s) 511, 866
BstBI TTCGAA 1 cut(s) 526
BstC8I GCNNGC 4 cut(s) 774, 921, 996, 1244
BstDEI CTNAG 4 cut(s) 44, 474, 577, 628
BstDSI CCRYGG 3 cut(s) 35, 429, 1286
BstF5I GGATG 3 cut(s) 65, 403, 1237
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 9 cut(s) 119, 424, 467, 602, 700, 835, 890, 955, 1039
BstMBI GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
BstMWI GCNNNNNNNGC 8 cut(s) 135, 144, 236, 826, 866, 869, 935, 1004
BstNI CCWGG 3 cut(s) 241, 840, 1028
BstSCI CCNGG 3 cut(s) 239, 838, 1026
BstSLI GKGCMC 1 cut(s) 1000
BstSNI TACGTA 1 cut(s) 511
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 4 cut(s) 359, 856, 872, 922
BstV2I GAAGAC 2 cut(s) 701, 909
BstX2I RGATCY 1 cut(s) 464
BstYI RGATCY 1 cut(s) 464
Bsu15I ATCGAT 1 cut(s) 882
BsuRI GGCC 3 cut(s) 239, 820, 1246
BsuTUI ATCGAT 1 cut(s) 882
BtgI CCRYGG 3 cut(s) 35, 429, 1286
BtsCI GGATG 3 cut(s) 65, 403, 1237
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 2 cut(s) 175, 913
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 4 cut(s) 774, 921, 996, 1244
CciI TCATGA 1 cut(s) 694
CfoI GCGC 1 cut(s) 152
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 2 cut(s) 595, 818
Csp6I GTAC 2 cut(s) 508, 974
CviAII CATG 6 cut(s) 36, 120, 430, 695, 830, 1287
CviQI GTAC 2 cut(s) 508, 974
DdeI CTNAG 4 cut(s) 44, 474, 577, 628
DpnI GATC 9 cut(s) 118, 423, 466, 601, 699, 834, 889, 954, 1038
DpnII GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
EaeI YGGCCR 1 cut(s) 818
Eam1104I CTCTTC 5 cut(s) 28, 82, 100, 138, 460
EarI CTCTTC 5 cut(s) 28, 82, 100, 138, 460
Eco105I TACGTA 1 cut(s) 511
Eco130I CCWWGG 3 cut(s) 35, 429, 1286
Eco24I GRGCYC 1 cut(s) 565
Eco57I CTGAAG 1 cut(s) 363
Eco72I CACGTG 1 cut(s) 866
Eco88I CYCGRG 2 cut(s) 142, 714
EcoRII CCWGG 3 cut(s) 239, 838, 1026
EcoT14I CCWWGG 3 cut(s) 35, 429, 1286
EcoT38I GRGCYC 1 cut(s) 565
ErhI CCWWGG 3 cut(s) 35, 429, 1286
FaeI CATG 6 cut(s) 39, 123, 433, 698, 833, 1290
FatI CATG 6 cut(s) 35, 119, 429, 694, 829, 1286
FbaI TGATCA 1 cut(s) 832
Fnu4HI GCNGC 6 cut(s) 148, 348, 861, 870, 936, 1188
FokI GGATG 3 cut(s) 52, 410, 1244
FriOI GRGCYC 1 cut(s) 565
Fsp4HI GCNGC 6 cut(s) 148, 348, 861, 870, 936, 1188
FspBI CTAG 2 cut(s) 950, 969
GlaI GCGC 1 cut(s) 151
GluI GCNGC 6 cut(s) 148, 348, 861, 870, 936, 1188
HaeIII GGCC 3 cut(s) 239, 820, 1246
HapII CCGG 2 cut(s) 210, 392
HgaI GACGC 2 cut(s) 595, 818
HhaI GCGC 1 cut(s) 152
Hin1II CATG 6 cut(s) 39, 123, 433, 698, 833, 1290
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 1167
HindII GTYRAC 1 cut(s) 1167
HinfI GANTC 7 cut(s) 40, 203, 359, 554, 743, 1094, 1160
HpaI GTTAAC 1 cut(s) 1167
HpaII CCGG 2 cut(s) 210, 392
HphI GGTGA 4 cut(s) 211, 431, 437, 594
Hpy166II GTNNAC 5 cut(s) 757, 974, 1013, 1139, 1167
Hpy188I TCNGA 8 cut(s) 277, 553, 631, 748, 1041, 1159, 1224, 1282
Hpy188III TCNNGA 9 cut(s) 46, 106, 142, 200, 210, 597, 695, 853, 1121
Hpy8I GTNNAC 5 cut(s) 757, 974, 1013, 1139, 1167
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 5 cut(s) 522, 799, 925, 1036, 1164
HpyCH4III ACNGT 2 cut(s) 908, 1171
HpyCH4IV ACGT 4 cut(s) 162, 510, 865, 1015
HpyCH4V TGCA 4 cut(s) 347, 539, 608, 923
HpyF10VI GCNNNNNNNGC 8 cut(s) 135, 144, 236, 826, 866, 869, 935, 1004
HpyF3I CTNAG 4 cut(s) 44, 474, 577, 628
HpySE526I ACGT 4 cut(s) 162, 510, 865, 1015
Hsp92II CATG 6 cut(s) 39, 123, 433, 698, 833, 1290
HspAI GCGC 1 cut(s) 150
Kpn2I TCCGGA 1 cut(s) 209
Ksp22I TGATCA 1 cut(s) 832
KspAI GTTAAC 1 cut(s) 1167
Kzo9I GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
LmnI GCTCC 2 cut(s) 192, 1239
Lsp1109I GCAGC 4 cut(s) 359, 856, 872, 922
LweI GCATC 5 cut(s) 184, 388, 598, 932, 1174
MaeI CTAG 2 cut(s) 950, 969
MaeII ACGT 4 cut(s) 162, 510, 865, 1015
MaeIII GTNAC 3 cut(s) 170, 369, 1271
MalI GATC 9 cut(s) 118, 423, 466, 601, 699, 834, 889, 954, 1038
MboI GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
MflI RGATCY 1 cut(s) 464
MhlI GDGCHC 3 cut(s) 189, 565, 1000
MluCI AATT 9 cut(s) 178, 261, 479, 633, 799, 844, 1046, 1196, 1218
MlyI GAGTC 1 cut(s) 353
MmeI TCCRAC 2 cut(s) 465, 1217
MroI TCCGGA 1 cut(s) 209
MroXI GAANNNNTTC 1 cut(s) 848
MseI TTAA 4 cut(s) 794, 1049, 1166, 1212
MspI CCGG 2 cut(s) 210, 392
MspR9I CCNGG 3 cut(s) 241, 840, 1028
Mva1269I GAATGC 2 cut(s) 812, 1062
MvaI CCWGG 3 cut(s) 241, 840, 1028
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 8 cut(s) 135, 144, 236, 826, 866, 869, 935, 1004
NcoI CCATGG 3 cut(s) 35, 429, 1286
NdeII GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
NlaIII CATG 6 cut(s) 39, 123, 433, 698, 833, 1290
NlaIV GGNNCC 1 cut(s) 214
NmuCI GTSAC 2 cut(s) 170, 369
NspV TTCGAA 1 cut(s) 526
PaeR7I CTCGAG 1 cut(s) 142
PagI TCATGA 1 cut(s) 694
PaqCI CACCTGC 1 cut(s) 613
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 198
PctI GAATGC 2 cut(s) 812, 1062
PdmI GAANNNNTTC 1 cut(s) 848
PfeI GAWTC 6 cut(s) 40, 203, 554, 743, 1094, 1160
PfoI TCCNGGA 1 cut(s) 1026
PkrI GCNGC 6 cut(s) 149, 349, 862, 871, 937, 1189
PleI GAGTC 1 cut(s) 353
PmaCI CACGTG 1 cut(s) 866
PmlI CACGTG 1 cut(s) 866
PpsI GAGTC 1 cut(s) 353
Ppu21I YACGTR 2 cut(s) 511, 866
Psp6I CCWGG 3 cut(s) 239, 838, 1026
PspCI CACGTG 1 cut(s) 866
PspGI CCWGG 3 cut(s) 239, 838, 1026
PspN4I GGNNCC 1 cut(s) 214
PsuI RGATCY 1 cut(s) 464
RsaI GTAC 2 cut(s) 509, 975
RsaNI GTAC 2 cut(s) 508, 974
SaqAI TTAA 4 cut(s) 794, 1049, 1166, 1212
SatI GCNGC 6 cut(s) 148, 348, 861, 870, 936, 1188
Sau3AI GATC 9 cut(s) 116, 421, 464, 599, 697, 832, 887, 952, 1036
SchI GAGTC 1 cut(s) 353
ScrFI CCNGG 3 cut(s) 241, 840, 1028
SduI GDGCHC 3 cut(s) 189, 565, 1000
SfaNI GCATC 5 cut(s) 184, 388, 598, 932, 1174
Sfr274I CTCGAG 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 526
SlaI CTCGAG 1 cut(s) 142
SmlI CTYRAG 2 cut(s) 142, 853
SmoI CTYRAG 2 cut(s) 142, 853
SnaBI TACGTA 1 cut(s) 511
SpeI ACTAGT 1 cut(s) 968
Sse9I AATT 9 cut(s) 178, 261, 479, 633, 799, 844, 1046, 1196, 1218
SsiI CCGC 3 cut(s) 148, 386, 1188
SspMI CTAG 2 cut(s) 950, 969
StyD4I CCNGG 3 cut(s) 239, 838, 1026
StyI CCWWGG 3 cut(s) 35, 429, 1286
TaaI ACNGT 2 cut(s) 908, 1171
TaiI ACGT 4 cut(s) 165, 513, 868, 1018
TaqI TCGA 6 cut(s) 107, 143, 201, 526, 672, 882
TasI AATT 9 cut(s) 178, 261, 479, 633, 799, 844, 1046, 1196, 1218
TatI WGTACW 1 cut(s) 973
TauI GCSGC 2 cut(s) 150, 1190
TfiI GAWTC 6 cut(s) 40, 203, 554, 743, 1094, 1160
Tru1I TTAA 4 cut(s) 794, 1049, 1166, 1212
Tru9I TTAA 4 cut(s) 794, 1049, 1166, 1212
TscAI CASTG 2 cut(s) 175, 913
TseFI GTSAC 2 cut(s) 170, 369
TseI GCWGC 4 cut(s) 347, 860, 869, 935
Tsp45I GTSAC 2 cut(s) 170, 369
TspDTI ATGAA 4 cut(s) 69, 447, 692, 915
TspGWI ACGGA 1 cut(s) 1159
TspRI CASTG 2 cut(s) 175, 913
XapI RAATTY 4 cut(s) 261, 633, 799, 844
XcmI CCANNNNNNNNNTGG 1 cut(s) 161
XhoI CTCGAG 1 cut(s) 142
XmnI GAANNNNTTC 1 cut(s) 848
XspI CTAG 2 cut(s) 950, 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.