pycom03g22340
ERF Family

Belongs to the GST superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003136
Physical Location & Seq
Reverse (-)
22484661 .. 22486019
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 792 bp
ATGGGTGCATACGATATCCCCTTGAGCCAAAAGCACTTGATCCTTCTCAGCAATGGTCATTGTCTTATAAATATTAGACCATATCAGGGTCCAAATCACAACACCTCTTTCTCAGTTGCTCAAATTTCTGACTTCAATTCCTCCAGAGTCATGGTTGCCATCAAGGTCCACGGAAGCGTTATTTCAACCGCTGCAATGCGAGTTTTGGCTACTCTCTACGAGAAAGACATCAAATTCGAGCTTGTTCCAATCGACATGCGAGCTGGTGAACACAAAAAGGAGCCGTTCATTTCCCTCAATCCATTTGGTCAAGTTCCAGCTTTTGAAGACGGAGATCTTAAGCTCTTTGAATCAAGGGCAATTACACAATACATTGCCCACGAGTATGCTGACAAGGGAACCCCTCTAGTGATCCGAGACTCGAAGAAGATGGCAATCATATCATTGTGGTCGGAGGTGGAGGGCCAAAAGTTCGACCCGGCGGCCTCAAAACTGACCTTTGAACTAGCTTTAAAGCCTATGTTTAAAATGACCACAAACGCGGCAGTTGTGGAGGAAAATGAAGCCAAGTTGGCTGTGGTTCTTGATGTATATGAGACTCGTCTGGCTCAGTCGAAATATTTGGCAGGTGAAAGCTTCACTTTGGCTGATCTTCACCACCTTCCCACCATACATTACTTGATGGGAACACAATCGAAGAAGCTGTTCGAATCCCGCCCCCATGTTCGCGCATGGGTGGCGGATATCACAGCAAGGCCAGCTTGGAACAAAGTCCTTGCCCAGCAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00480 ko00980 ko00982 ko00983 ko01524 ko05200 ko05204 ko05225 ko05418 map00480 map00980 map00982 map00983 map01524 map05200 map05204 map05225 map05418
Pfam Domains
Protein Families
GST_C GST_N

Protein Analysis

264

Amino Acids

29.48

Weight (kDa)

8.48

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_3 PF13417 57 - 131 3.3e-13 Glutathione S-transferase, N-terminal domain
GST_N PF02798 59 - 126 4.9e-20 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 62 - 127 1.3e-11 Glutathione S-transferase, N-terminal domain
GST_C PF00043 167 - 253 3.8e-17 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 182 - 247 2.8e-06 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 187 - 255 2e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 68
AarI CACCTGC 1 cut(s) 617
Acc36I ACCTGC 1 cut(s) 617
AccII CGCG 2 cut(s) 542, 729
AciI CCGC 5 cut(s) 189, 482, 542, 715, 740
AclWI GGATC 2 cut(s) 34, 406
AcsI RAATTY 2 cut(s) 123, 233
AfiI CCNNNNNNNGG 1 cut(s) 86
AflII CTTAAG 1 cut(s) 338
AgsI TTSAA 5 cut(s) 136, 186, 326, 350, 503
AluBI AGCT 8 cut(s) 241, 263, 320, 343, 509, 636, 703, 761
AluI AGCT 8 cut(s) 241, 263, 320, 343, 509, 636, 703, 761
Alw26I GTCTC 2 cut(s) 411, 590
AlwI GGATC 2 cut(s) 34, 406
AoxI GGCC 3 cut(s) 463, 483, 755
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 2 cut(s) 123, 233
ArsI GACNNNNNNTTYG 2 cut(s) 218, 250
Asp700I GAANNNNTTC 1 cut(s) 704
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 3 cut(s) 89, 166, 463
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 3 cut(s) 278, 641, 647
AsuII TTCGAA 1 cut(s) 708
AvaII GGWCC 2 cut(s) 89, 166
BauI CACGAG 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 178
BccI CCATC 3 cut(s) 167, 424, 676
BceAI ACGGC 1 cut(s) 268
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 411, 590
BfaI CTAG 2 cut(s) 407, 506
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 617
BglI GCCNNNNNGGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 334
BisI GCNGC 3 cut(s) 192, 483, 543
BlsI GCNGC 3 cut(s) 193, 484, 544
Bme1390I CCNGG 1 cut(s) 479
Bme18I GGWCC 2 cut(s) 89, 166
BmgT120I GGNCC 3 cut(s) 89, 166, 463
BmiI GGNNCC 3 cut(s) 90, 282, 400
BmrFI CCNGG 1 cut(s) 479
BpiI GAAGAC 1 cut(s) 333
BpmI CTGGAG 1 cut(s) 127
Bpu14I TTCGAA 1 cut(s) 708
BpuEI CTTGAG 1 cut(s) 43
BpuMI CCSGG 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 434
BsaJI CCNNGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 1 cut(s) 86
Bse3DI GCAATG 3 cut(s) 58, 201, 372
Bse8I GATNNNNATC 1 cut(s) 434
BseDI CCNNGG 1 cut(s) 169
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 1 cut(s) 86
BseMI GCAATG 3 cut(s) 58, 201, 372
BseMII CTCAG 3 cut(s) 61, 126, 623
BseXI GCAGC 1 cut(s) 178
BseYI CCCAGC 1 cut(s) 780
Bsh1236I CGCG 2 cut(s) 542, 729
BshFI GGCC 3 cut(s) 465, 485, 757
BsiSI CCGG 1 cut(s) 479
BslI CCNNNNNNNGG 1 cut(s) 86
BsmAI GTCTC 2 cut(s) 411, 590
BsnI GGCC 3 cut(s) 465, 485, 757
Bsp119I TTCGAA 1 cut(s) 708
Bsp143I GATC 4 cut(s) 39, 334, 411, 649
BspACI CCGC 5 cut(s) 189, 482, 542, 715, 740
BspANI GGCC 3 cut(s) 465, 485, 757
BspCNI CTCAG 3 cut(s) 60, 125, 622
BspFNI CGCG 2 cut(s) 542, 729
BspLI GGNNCC 3 cut(s) 90, 282, 400
BspMI ACCTGC 1 cut(s) 617
BspPI GGATC 2 cut(s) 34, 406
BspT104I TTCGAA 1 cut(s) 708
BspTI CTTAAG 1 cut(s) 338
BsrDI GCAATG 3 cut(s) 58, 201, 372
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 4 cut(s) 39, 334, 411, 649
BssSI CACGAG 1 cut(s) 380
Bst2BI CACGAG 1 cut(s) 380
BstAFI CTTAAG 1 cut(s) 338
BstBI TTCGAA 1 cut(s) 708
BstC8I GCNNGC 2 cut(s) 261, 759
BstDEI CTNAG 3 cut(s) 47, 112, 609
BstDSI CCRYGG 1 cut(s) 169
BstFNI CGCG 2 cut(s) 542, 729
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 4 cut(s) 42, 337, 414, 652
BstMAI GTCTC 2 cut(s) 411, 590
BstMBI GATC 4 cut(s) 39, 334, 411, 649
BstMWI GCNNNNNNNGC 3 cut(s) 572, 737, 758
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 477
BstUI CGCG 2 cut(s) 542, 729
BstV1I GCAGC 1 cut(s) 178
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 1 cut(s) 334
BstXI CCANNNNNNTGG 1 cut(s) 151
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 3 cut(s) 465, 485, 757
BtgI CCRYGG 1 cut(s) 169
BveI ACCTGC 1 cut(s) 617
Cac8I GCNNGC 2 cut(s) 261, 759
CfoI GCGC 1 cut(s) 731
Cfr13I GGNCC 3 cut(s) 89, 166, 463
CviAII CATG 4 cut(s) 151, 256, 722, 732
DdeI CTNAG 3 cut(s) 47, 112, 609
DpnI GATC 4 cut(s) 41, 336, 413, 651
DpnII GATC 4 cut(s) 39, 334, 411, 649
DraI TTTAAA 2 cut(s) 513, 526
EciI GGCGGA 1 cut(s) 755
Eco32I GATATC 2 cut(s) 16, 745
Eco47I GGWCC 2 cut(s) 89, 166
EcoRV GATATC 2 cut(s) 16, 745
FaeI CATG 4 cut(s) 154, 259, 725, 735
FalI AAGNNNNNCTT 4 cut(s) 625, 657, 745, 777
FatI CATG 4 cut(s) 150, 255, 721, 731
FauI CCCGC 1 cut(s) 722
Fnu4HI GCNGC 3 cut(s) 192, 483, 543
Fsp4HI GCNGC 3 cut(s) 192, 483, 543
FspBI CTAG 2 cut(s) 407, 506
GlaI GCGC 1 cut(s) 730
GluI GCNGC 3 cut(s) 192, 483, 543
GsaI CCCAGC 1 cut(s) 784
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 3 cut(s) 465, 485, 757
HapII CCGG 1 cut(s) 479
HhaI GCGC 1 cut(s) 731
Hin1II CATG 4 cut(s) 154, 259, 725, 735
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 634
HinfI GANTC 5 cut(s) 147, 350, 419, 598, 710
HpaII CCGG 1 cut(s) 479
HphI GGTGA 3 cut(s) 278, 641, 647
Hpy166II GTNNAC 2 cut(s) 169, 269
Hpy188I TCNGA 3 cut(s) 130, 416, 454
Hpy188III TCNNGA 2 cut(s) 144, 584
Hpy8I GTNNAC 2 cut(s) 169, 269
HpyAV CCTTC 2 cut(s) 53, 671
HpyCH4V TGCA 2 cut(s) 8, 194
HpyF10VI GCNNNNNNNGC 3 cut(s) 572, 737, 758
HpyF3I CTNAG 3 cut(s) 47, 112, 609
Hsp92II CATG 4 cut(s) 154, 259, 725, 735
HspAI GCGC 1 cut(s) 729
Kzo9I GATC 4 cut(s) 39, 334, 411, 649
LmnI GCTCC 1 cut(s) 280
LpnPI CCDG 8 cut(s) 71, 157, 249, 330, 492, 590, 612, 771
Lsp1109I GCAGC 1 cut(s) 178
MaeI CTAG 2 cut(s) 407, 506
MalI GATC 4 cut(s) 41, 336, 413, 651
MboI GATC 4 cut(s) 39, 334, 411, 649
MboII GAAGA 5 cut(s) 338, 436, 439, 644, 709
MflI RGATCY 1 cut(s) 334
MluCI AATT 4 cut(s) 123, 136, 233, 360
MlyI GAGTC 3 cut(s) 156, 413, 592
MmeI TCCRAC 1 cut(s) 432
MnlI CCTC 8 cut(s) 115, 151, 305, 414, 448, 454, 496, 547
MroXI GAANNNNTTC 1 cut(s) 704
MseI TTAA 3 cut(s) 339, 512, 525
MslI CAYNNNNRTG 1 cut(s) 384
MspA1I CMGCKG 1 cut(s) 191
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 1 cut(s) 479
MspR9I CCNGG 1 cut(s) 479
MvnI CGCG 2 cut(s) 542, 729
MwoI GCNNNNNNNGC 3 cut(s) 572, 737, 758
NciI CCSGG 1 cut(s) 479
NdeII GATC 4 cut(s) 39, 334, 411, 649
NlaIII CATG 4 cut(s) 154, 259, 725, 735
NlaIV GGNNCC 3 cut(s) 90, 282, 400
NspI RCATGY 1 cut(s) 259
NspV TTCGAA 1 cut(s) 708
PaqCI CACCTGC 1 cut(s) 617
PdmI GAANNNNTTC 1 cut(s) 704
PfeI GAWTC 2 cut(s) 350, 710
PkrI GCNGC 3 cut(s) 193, 484, 544
PleI GAGTC 3 cut(s) 155, 413, 592
PpsI GAGTC 3 cut(s) 155, 413, 592
PsiI TTATAA 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 780
PspN4I GGNNCC 3 cut(s) 90, 282, 400
PspPI GGNCC 3 cut(s) 89, 166, 463
PsuI RGATCY 1 cut(s) 334
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 3 cut(s) 339, 512, 525
SatI GCNGC 3 cut(s) 192, 483, 543
Sau3AI GATC 4 cut(s) 39, 334, 411, 649
Sau96I GGNCC 3 cut(s) 89, 166, 463
SchI GAGTC 3 cut(s) 156, 413, 592
ScrFI CCNGG 1 cut(s) 479
SfuI TTCGAA 1 cut(s) 708
SinI GGWCC 2 cut(s) 89, 166
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 2 cut(s) 22, 338
SmoI CTYRAG 2 cut(s) 22, 338
Sse9I AATT 4 cut(s) 123, 136, 233, 360
SsiI CCGC 5 cut(s) 189, 482, 542, 715, 740
SspI AATATT 2 cut(s) 73, 620
SspMI CTAG 2 cut(s) 407, 506
StyD4I CCNGG 1 cut(s) 477
TaqI TCGA 7 cut(s) 237, 252, 422, 474, 614, 695, 708
TasI AATT 4 cut(s) 123, 136, 233, 360
TauI GCSGC 2 cut(s) 485, 545
TfiI GAWTC 2 cut(s) 350, 710
Tru1I TTAA 3 cut(s) 339, 512, 525
Tru9I TTAA 3 cut(s) 339, 512, 525
TseI GCWGC 1 cut(s) 191
TspDTI ATGAA 2 cut(s) 277, 576
TspGWI ACGGA 2 cut(s) 186, 345
Vha464I CTTAAG 1 cut(s) 338
VpaK11BI GGWCC 2 cut(s) 89, 166
XapI RAATTY 2 cut(s) 123, 233
XceI RCATGY 1 cut(s) 259
XcmI CCANNNNNNNNNTGG 1 cut(s) 574
XmnI GAANNNNTTC 1 cut(s) 704
XspI CTAG 2 cut(s) 407, 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.