pycom04g04240

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0008695
Physical Location & Seq
Reverse (-)
3801059 .. 3801292
234 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g04240.1

Sequence Viewer

Length: 234 bp
ATGACTTTCCAGAAAATGAGCAGCCTCCTATTGATCATCTTCGTGTTGTCGGTGGCCATTTTGGTCAGCCAAGGTCCGGTTGAAGCGAGCAGAGTGTTGTCGGAGGACTTTGCCGGTGCTAACCACTTGGAGACTTACTCGTCGCCTTCTGTGTATGAAAAGGCCAAGTTCACCATGGAATTTTGGCTTGAACGGTTAGCTTCGGGACCTAGCCCCAAGGGGCCTGGTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

78

Amino Acids

8.43

Weight (kDa)

6.04

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 139
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 179
AfiI CCNNNNNNNGG 1 cut(s) 76
AgsI TTSAA 2 cut(s) 83, 191
AjnI CCWGG 1 cut(s) 223
AluBI AGCT 1 cut(s) 200
AluI AGCT 1 cut(s) 200
Alw26I GTCTC 1 cut(s) 125
AoxI GGCC 3 cut(s) 54, 162, 221
ApeKI GCWGC 1 cut(s) 21
ApoI RAATTY 1 cut(s) 179
AspS9I GGNCC 3 cut(s) 74, 206, 221
AsuHPI GGTGA 1 cut(s) 163
AvaII GGWCC 2 cut(s) 74, 206
BalI TGGCCA 1 cut(s) 56
BbvI GCAGC 1 cut(s) 33
BciT130I CCWGG 1 cut(s) 225
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 1 cut(s) 210
BisI GCNGC 1 cut(s) 22
BlsI GCNGC 1 cut(s) 23
Bme1390I CCNGG 1 cut(s) 225
Bme18I GGWCC 2 cut(s) 74, 206
BmgT120I GGNCC 3 cut(s) 74, 206, 221
BmiI GGNNCC 2 cut(s) 207, 222
BmrFI CCNGG 1 cut(s) 225
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BsaJI CCNNGG 3 cut(s) 70, 174, 216
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 1 cut(s) 76
Bse118I RCCGGY 1 cut(s) 113
BseBI CCWGG 1 cut(s) 225
BseDI CCNNGG 3 cut(s) 70, 174, 216
BseLI CCNNNNNNNGG 1 cut(s) 76
BseXI GCAGC 1 cut(s) 33
BshFI GGCC 3 cut(s) 56, 164, 223
BsiSI CCGG 2 cut(s) 77, 114
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 76
BsmAI GTCTC 1 cut(s) 125
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 3 cut(s) 56, 164, 223
Bsp143I GATC 1 cut(s) 33
Bsp19I CCATGG 1 cut(s) 174
BspANI GGCC 3 cut(s) 56, 164, 223
BspLI GGNNCC 2 cut(s) 207, 222
BsrFI RCCGGY 1 cut(s) 113
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 3 cut(s) 70, 174, 216
BssMI GATC 1 cut(s) 33
BssT1I CCWWGG 3 cut(s) 70, 174, 216
Bst2UI CCWGG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 88
BstDSI CCRYGG 1 cut(s) 174
BstKTI GATC 1 cut(s) 36
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 1 cut(s) 33
BstNI CCWGG 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 223
BstV1I GCAGC 1 cut(s) 33
BsuRI GGCC 3 cut(s) 56, 164, 223
BtgI CCRYGG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 3 cut(s) 74, 206, 221
CviAII CATG 1 cut(s) 175
CviJI RGCY 8 cut(s) 24, 56, 69, 164, 187, 200, 213, 223
CviKI_1 RGCY 8 cut(s) 24, 56, 69, 164, 187, 200, 213, 223
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
DrdI GACNNNNNNGTC 1 cut(s) 139
DseDI GACNNNNNNGTC 1 cut(s) 139
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 3 cut(s) 70, 174, 216
Eco47I GGWCC 2 cut(s) 74, 206
EcoO109I RGGNCCY 2 cut(s) 206, 221
EcoRII CCWGG 1 cut(s) 223
EcoT14I CCWWGG 3 cut(s) 70, 174, 216
ErhI CCWWGG 3 cut(s) 70, 174, 216
FaeI CATG 1 cut(s) 178
FaiI YATR 2 cut(s) 156, 176
FaqI GGGAC 1 cut(s) 219
FatI CATG 1 cut(s) 174
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 22
Fsp4HI GCNGC 1 cut(s) 22
FspBI CTAG 1 cut(s) 210
GluI GCNGC 1 cut(s) 22
HaeIII GGCC 3 cut(s) 56, 164, 223
HapII CCGG 2 cut(s) 77, 114
Hin1II CATG 1 cut(s) 178
HpaII CCGG 2 cut(s) 77, 114
HphI GGTGA 1 cut(s) 163
Hpy166II GTNNAC 1 cut(s) 171
Hpy188I TCNGA 1 cut(s) 103
Hpy188III TCNNGA 2 cut(s) 10, 204
Hpy8I GTNNAC 1 cut(s) 171
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 1 cut(s) 156
HpyCH4III ACNGT 1 cut(s) 195
Hsp92II CATG 1 cut(s) 178
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 1 cut(s) 33
LpnPI CCDG 4 cut(s) 23, 90, 127, 210
Lsp1109I GCAGC 1 cut(s) 33
MaeI CTAG 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 227
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 31
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 1 cut(s) 179
MluNI TGGCCA 1 cut(s) 56
MmeI TCCRAC 1 cut(s) 81
MnlI CCTC 2 cut(s) 35, 97
Mox20I TGGCCA 1 cut(s) 56
MscI TGGCCA 1 cut(s) 56
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 2 cut(s) 77, 114
MspR9I CCNGG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 225
NcoI CCATGG 1 cut(s) 174
NdeII GATC 1 cut(s) 33
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 2 cut(s) 207, 222
NmuCI GTSAC 1 cut(s) 227
PkrI GCNGC 1 cut(s) 23
PpuMI RGGWCCY 1 cut(s) 206
Psp5II RGGWCCY 1 cut(s) 206
Psp6I CCWGG 1 cut(s) 223
PspGI CCWGG 1 cut(s) 223
PspN4I GGNNCC 2 cut(s) 207, 222
PspPI GGNCC 3 cut(s) 74, 206, 221
PspPPI RGGWCCY 1 cut(s) 206
RseI CAYNNNNRTG 1 cut(s) 41
SatI GCNGC 1 cut(s) 22
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 3 cut(s) 74, 206, 221
ScrFI CCNGG 1 cut(s) 225
SetI ASST 3 cut(s) 76, 202, 211
SinI GGWCC 2 cut(s) 74, 206
SmiMI CAYNNNNRTG 1 cut(s) 41
Sse9I AATT 1 cut(s) 179
SspMI CTAG 1 cut(s) 210
StyD4I CCNGG 1 cut(s) 223
StyI CCWWGG 3 cut(s) 70, 174, 216
TaaI ACNGT 1 cut(s) 195
TasI AATT 1 cut(s) 179
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 1 cut(s) 21
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 1 cut(s) 171
VpaK11BI GGWCC 2 cut(s) 74, 206
XapI RAATTY 1 cut(s) 179
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XspI CTAG 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.