RLG00000008779

Membrane-associated 30 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008695
Physical Location & Seq
Forward (+)
39077286 .. 39081749
4464 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008779

Sequence Viewer

Length: 540 bp
ATGTCAGATGTACACACGGCCTCCTTCCCTAAAGAGGAGAACCCAAGCAGTAGTTACCTTCCGAAGGCCTATAGGTATTGGCATCTCAAAACACTAGAGAACAAGATACAAGAGGAAAGGTCAAAAGAAGATACACTCAAGGCATGTGCCCAGTCTGCAAGGACTGCTACTAAAGTGAGTGAGATGGTGGGGAATGTTGATGTAGGTCATGCCCTTTCTGCTTTTGAAAAGATAGAAGAGAAAGGTAACATCCAGGTGGCTTCAGACTTCAAAACAATTAACAATTTCAGACTTTTCTTGCTCAGTCAGTTCAGAAACAAGAACTGGGTCAATGGCCTGGGTAGTAGTCAATTCACATATTCCAAGCATTGGACGGAATTAATAATCAAATTTAACTCCTTGGTCAACACGTATGGAGTAGCCCGCCAAAACAGAGACCTACAAGTCAAGTTGATCGACGAAGTGCTGTCTCACCAGCCACACTATATATACATCAACACAACAAAGGCCTCCTCATCCATATCATCCACTTCCAATTAA

Protein Analysis

180

Amino Acids

20.41

Weight (kDa)

9.1

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PspA_IM30 PF04012 29 - 81 1.8e-08 PspA/IM30 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 443
AccB7I CCANNNNNTGG 1 cut(s) 369
AciI CCGC 1 cut(s) 424
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 12
AfiI CCNNNNNNNGG 3 cut(s) 34, 64, 369
AflIII ACRYGT 1 cut(s) 408
AgsI TTSAA 2 cut(s) 227, 271
AjnI CCWGG 2 cut(s) 252, 336
Alw26I GTCTC 2 cut(s) 429, 474
AoxI GGCC 4 cut(s) 18, 66, 334, 507
ApoI RAATTY 1 cut(s) 389
AseI ATTAAT 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 464
BaeGI GKGCMC 1 cut(s) 151
BccI CCATC 1 cut(s) 178
BceAI ACGGC 1 cut(s) 33
BciT130I CCWGG 2 cut(s) 254, 338
BcoDI GTCTC 2 cut(s) 429, 474
BfaI CTAG 1 cut(s) 95
BfmI CTRYAG 1 cut(s) 70
Bme1390I CCNGG 2 cut(s) 254, 338
BmrFI CCNGG 2 cut(s) 254, 338
BmrI ACTGGG 2 cut(s) 145, 334
BmsI GCATC 1 cut(s) 91
BmuI ACTGGG 2 cut(s) 145, 334
BpuEI CTTGAG 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 411
BsaI GGTCTC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 337, 399
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 64, 369
Bse1I ACTGG 2 cut(s) 151, 329
BseBI CCWGG 2 cut(s) 254, 338
BseDI CCNNGG 2 cut(s) 337, 399
BseGI GGATG 3 cut(s) 249, 515, 524
BseLI CCNNNNNNNGG 3 cut(s) 34, 64, 369
BseMII CTCAG 1 cut(s) 316
BseNI ACTGG 2 cut(s) 151, 329
BseRI GAGGAG 2 cut(s) 50, 502
BseSI GKGCMC 1 cut(s) 151
BshFI GGCC 4 cut(s) 20, 68, 336, 509
BslI CCNNNNNNNGG 3 cut(s) 34, 64, 369
BsmAI GTCTC 2 cut(s) 429, 474
BsnI GGCC 4 cut(s) 20, 68, 336, 509
Bso31I GGTCTC 1 cut(s) 429
Bsp1286I GDGCHC 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 10
Bsp143I GATC 1 cut(s) 453
BspACI CCGC 1 cut(s) 424
BspANI GGCC 4 cut(s) 20, 68, 336, 509
BspCNI CTCAG 1 cut(s) 315
BspTNI GGTCTC 1 cut(s) 429
BsrGI TGTACA 1 cut(s) 10
BsrI ACTGG 2 cut(s) 151, 329
BssECI CCNNGG 2 cut(s) 337, 399
BssMI GATC 1 cut(s) 453
BssT1I CCWWGG 1 cut(s) 399
Bst2UI CCWGG 2 cut(s) 254, 338
Bst6I CTCTTC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 164
BstAUI TGTACA 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 411
BstC8I GCNNGC 1 cut(s) 424
BstDEI CTNAG 1 cut(s) 302
BstENI CCTNNNNNAGG 1 cut(s) 62
BstF5I GGATG 3 cut(s) 249, 515, 524
BstKTI GATC 1 cut(s) 456
BstMAI GTCTC 2 cut(s) 429, 474
BstMBI GATC 1 cut(s) 453
BstMWI GCNNNNNNNGC 3 cut(s) 155, 164, 218
BstNI CCWGG 2 cut(s) 254, 338
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 2 cut(s) 252, 336
BstSFI CTRYAG 1 cut(s) 70
BstSLI GKGCMC 1 cut(s) 151
BsuRI GGCC 4 cut(s) 20, 68, 336, 509
BtsCI GGATG 3 cut(s) 249, 515, 524
Cac8I GCNNGC 1 cut(s) 424
Csp6I GTAC 1 cut(s) 11
CviAII CATG 2 cut(s) 144, 209
CviJI RGCY 7 cut(s) 20, 68, 260, 336, 422, 478, 509
CviKI_1 RGCY 7 cut(s) 20, 68, 260, 336, 422, 478, 509
CviQI GTAC 1 cut(s) 11
DdeI CTNAG 1 cut(s) 302
DpnI GATC 1 cut(s) 455
DpnII GATC 1 cut(s) 453
DrdI GACNNNNNNGTC 1 cut(s) 443
DseDI GACNNNNNNGTC 1 cut(s) 443
Eam1104I CTCTTC 1 cut(s) 231
EarI CTCTTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 399
Eco147I AGGCCT 2 cut(s) 68, 509
Eco31I GGTCTC 1 cut(s) 429
Eco57I CTGAAG 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 62
EcoRII CCWGG 2 cut(s) 252, 336
EcoT14I CCWWGG 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 399
FaeI CATG 2 cut(s) 147, 212
FaiI YATR 9 cut(s) 72, 145, 210, 358, 414, 486, 488, 490, 521
FatI CATG 2 cut(s) 143, 208
FauI CCCGC 1 cut(s) 431
FokI GGATG 3 cut(s) 236, 502, 511
FspBI CTAG 1 cut(s) 95
HaeIII GGCC 4 cut(s) 20, 68, 336, 509
Hin1II CATG 2 cut(s) 147, 212
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HphI GGTGA 1 cut(s) 464
Hpy166II GTNNAC 2 cut(s) 13, 406
Hpy188I TCNGA 5 cut(s) 7, 63, 265, 290, 314
Hpy8I GTNNAC 2 cut(s) 13, 406
Hpy99I CGWCG 1 cut(s) 461
HpyAV CCTTC 3 cut(s) 34, 58, 68
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 1 cut(s) 158
HpyF10VI GCNNNNNNNGC 3 cut(s) 155, 164, 218
HpyF3I CTNAG 1 cut(s) 302
HpySE526I ACGT 1 cut(s) 410
Hsp92II CATG 2 cut(s) 147, 212
Kzo9I GATC 1 cut(s) 453
LpnPI CCDG 7 cut(s) 164, 239, 266, 310, 323, 350, 488
LweI GCATC 1 cut(s) 91
MaeI CTAG 1 cut(s) 95
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 2 cut(s) 53, 245
MalI GATC 1 cut(s) 455
MboI GATC 1 cut(s) 453
MboII GAAGA 2 cut(s) 140, 248
MhlI GDGCHC 1 cut(s) 151
MluCI AATT 6 cut(s) 276, 283, 350, 377, 389, 535
MnlI CCTC 5 cut(s) 28, 31, 106, 520, 523
MseI TTAA 4 cut(s) 279, 380, 393, 538
MslI CAYNNNNRTG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 254, 338
MvaI CCWGG 2 cut(s) 254, 338
MwoI GCNNNNNNNGC 3 cut(s) 155, 164, 218
NdeII GATC 1 cut(s) 453
NlaIII CATG 2 cut(s) 147, 212
NspI RCATGY 1 cut(s) 147
PceI AGGCCT 2 cut(s) 68, 509
PflMI CCANNNNNTGG 1 cut(s) 369
Ppu21I YACGTR 1 cut(s) 411
PshBI ATTAAT 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 252, 336
PspGI CCWGG 2 cut(s) 252, 336
RsaI GTAC 1 cut(s) 12
RsaNI GTAC 1 cut(s) 11
RseI CAYNNNNRTG 1 cut(s) 254
SaqAI TTAA 4 cut(s) 279, 380, 393, 538
Sau3AI GATC 1 cut(s) 453
ScrFI CCNGG 2 cut(s) 254, 338
SduI GDGCHC 1 cut(s) 151
SetI ASST 8 cut(s) 60, 77, 122, 208, 247, 258, 413, 441
SfaNI GCATC 1 cut(s) 91
SfcI CTRYAG 1 cut(s) 70
SmiMI CAYNNNNRTG 1 cut(s) 254
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 6 cut(s) 276, 283, 350, 377, 389, 535
SseBI AGGCCT 2 cut(s) 68, 509
SsiI CCGC 1 cut(s) 424
SspMI CTAG 1 cut(s) 95
StuI AGGCCT 2 cut(s) 68, 509
StyD4I CCNGG 2 cut(s) 252, 336
StyI CCWWGG 1 cut(s) 399
TaiI ACGT 1 cut(s) 413
TaqI TCGA 1 cut(s) 456
TasI AATT 6 cut(s) 276, 283, 350, 377, 389, 535
TatI WGTACW 1 cut(s) 10
Tru1I TTAA 4 cut(s) 279, 380, 393, 538
Tru9I TTAA 4 cut(s) 279, 380, 393, 538
TspGWI ACGGA 1 cut(s) 389
Van91I CCANNNNNTGG 1 cut(s) 369
VspI ATTAAT 1 cut(s) 380
XagI CCTNNNNNAGG 1 cut(s) 62
XapI RAATTY 1 cut(s) 389
XceI RCATGY 1 cut(s) 147
XspI CTAG 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.