pycom04g07060

ATP-dependent RNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005499
Physical Location & Seq
Forward (+)
7414363 .. 7426670
12308 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3471 bp
ATGCGTAATTCGAGAGCAGCACTTGGTCTCCTTCTCAAGTCAGTCTCCAACAATAACCCTTGTTGTTTTCCTTCTTCCCGCAAGACAAACGGTTGCTGCCGCACTTTGCTTCTTCTACTCCAACCCCACTCTGCTTCTTCAATTTCCGACGGCGGAGCCCGATTCCTCATTTCAAAGCGCGCCTTTTGCGGGTACGCCGCCGAGCAGTTCTCCGACGATGAGTACGCGTGCGAATTCGAAGGCCAAAAGGCATCGTCTTCAGTGGCGAACATTGACGAGTGGAAATGGAAAATGAGCTTGCTATTACGCAGCGAAAAGGACCAAGAGATTGTATCCAGAGATAAGAGAGATAGGAGAGACTATGAGCAGATATCTAACCTTGCCAAAAGGATGGGACTTTATTGTGAAATATATGGGAAAGCGGTGGTGGCCAGCAAGGTCCCTCTTCCAAACTACAGGCCTGATTTGGATGATAAGCGGCCACAAAGAGAGGTGGTTATCCCACTTGGCTTGCAAAGGAGGGTAGAGGGTTTGCTGCAAGAGCACCTCGATCGACTCCAGTTAAACTCTGGGAAGTTCACTGGAAATAGAGGGGACTCTGAGCACATTGGTCAGGTCGAAAATGCAAACCTGGATGAGAATGCAGATTCTTTTCTAGATGGGTCTGTAATGGAGAAGGTTCTTCAAAGGAGGAGTTTGCGAATGCGCAATATGCAAAGAGCTTGGCAGGAATCACCCGAAGGCAAGAAGATGCTAGACTTCCGCAAATCACTACCGGCTTTCAAGGAGAATGAAAGGTTGCTGCAAGCAATTGCACAGAATCAGGTAATAGTGATATCTGGGGAGACTGGATGTGGTAAGACCACTCAACTTCCTCAATATATCCTAGAGTCAGAAATAGAATCTGGCCGTGGAGCATTCTGTAGCATCATTTGCACACAGCCTCGAAGAATATCTGCTATGGCTGTTGCAGAAAGAGTGTCCGCAGAGAGGGGAGAGCCTCTTGGTGAAACAGTTGGCTATAAAGTTCGATTGGAGGGAATGAAGGGAAAAAATACCCATCTGCTTTTTTGTACAAGTGGTATTTTACTTCGCCGTCTGTTGAGTGATCGTAACTTGAATGGTATAACCCATGTTTTTGTTGATGAAATTCATGAGCGAGGCATGAATGAAGATTTCTTATTGATTGTGCTGAAGGATCTGCTTCCACGCCGTCGGGATTTGAGATTGGTTTTAATGAGTGCTACTCTGAATGCTGAGCTGTTTTCAAGTTATTTTGGAGGAGCTCCTACAATTCATATTCCGGGCTTTACATATCCAGTAAGAGCACACTTTTTAGAAGATGTACTGGAAATGACTGGGTATAAGTTGACTTCCTTCAACCAAATTGATGATTATGGCCAAGACAAACTGTGGAAAACACAGAAGCAGTTGGTGCCTCGAAAAAGGAAAAACCAGATTACTGCTCTGGTTGAGGATGCTCTTAATAAATCAAGCTTTGAGAGTTACAGTGCCAGGGCACGTGATTCACTATCATGTTGGACACCTGATTGCATTGGATTTAATTTAATTGAGGCAGTTTTATGTCATATATGTCGGAAGGAAAGACAAGGTGCTGTTTTAGTATTCATGACTGGGTGGGAGGATATAAGCTCTTTGAGGGATCAACTTAAAGCACATCCACTTTTAGGTGATCCTAACAGGGTTTTTCTTCTAACATGCCATGGGTCAATGGGAACATCTGAACAGAAACTCATATTTGGAAGACCACCTCCTAACATTCGTAAAATAGTCCTTGCAACAAATATGGCAGAAGCAAGTATTACAATCAATGATGTAGTTTTTGTGGTGGATTGTGGAAAAGCAAAAGAGACCTCGTATGATGCTTTGAATAATACACCTTGTCTGCTACCTTCTTGGATATCGCAAGCATCTGCTCGGCAAAGAAGGGGTAGGGCTGGCCGGGTGCTGCCAGGCGAATGTTACCACCTTTACCCCAAATGCGTTTATCACGCTTTTGCTGAATATCAACTTCCTGAACTGCTCAGAACTCCTTTGAACTCTCTTTGCTTGCAAATTAAAAGTTTGCAGGTTGCTAGTATAGGGGAATTCTTGTCAGCTGCCTTGCAGCCACCGGAACCCCTAGCTGTCCAAAATGCCATTGGTTTTCTTACTTCAATTGGGGCATTAGATGAGAATGAAAATTTGACAAGTCTCGGAAAATATTTGTCAATACTTCCAGTGGATCCCAAACTGGGGAAAATGCTGATTATGGGTGCTGTGTTTCATTGCTTTGATCCTGTTCTTACAATAGTGTCTGGTCTAAGTGTCAGGGATCCTTTCCTTTTGCCTCAAGACAAAAAAGATTTAGCAGGGACAGCAAAATCTAGATTCTCTGCAAAAGATTACAGTGATCATATGGCTCTTGTTCGTGCATATGAAGGATGGAAAGATGCTGAAAGAGAAGGATCAGCATATGAATATTGCTGGAGAAATTTCCTTTCTGCTCAAACACTTCAGGCTATCCATTCTCTTCGGAAGCAGTTTAACTACATTTTAAGAGATGCTGGATTGGTGGATGCGGATGCAAGCATTAATAACAAGCTAAGTCACAACCAGTCCTTGGTTCGTGCAATCATATGTTCTGGACTTTTCCCTGGAATAGCATCTGTGGTGCACCGAGAGACGTCCATGTCATTCAAAACGATGGATGATGGCCAGGTTCTACTATTTGCTAATTCTGTGAATGTGCGTTACCAAACAATTCCTTACCCCTGGTTGGTCTTTGGTGAGAAAGTAAGGGTCAATACTGTATTCATACGTGATTCCACTGGCGTATCTGATTCGATCCTGATCCTTTTTGGTGGTGCGTTGAACCATGGGGTCCAGGCTGGGCATTTGAGAATGTTGGATGGTTATATCGACTTCTTCATGGATCCTAGTTTGGTGGACTGCTATTTGAAGCTTAAGGAAGAACTTAATGAGCTTATTCAAAAGAAGCTTGAAGATCCTAGCCTTGATATCCACAAGGAAGGGAAGTACCTGATGCTTGCTGTTCAAGAGCTGGTTTCCGGAGATCAATGTGAAGGGAGATTTGTATTTGGTCGTGACAGCAGAAAGCCGAAGGAATCTGGTGATAACAGTAGATTCACTAAAGACGGAACCAATCCTAAGAGTTTGCTTCAAACTCTGTTGATGAGAGCAGGACATTCTCCTCCAAAATACAAAACAAAGCACCTTAAGACGAACGAGTTCAGAGCACTGGTGGAGTTCAAGGGCATGCAATTTGTCGGGAAACCTAAGAAAAATAAGCAGCTTGCAGAAAGGGATGCTGCTATAGAAGCACTGGCCTGGTTGACTCACACCTCAGAAAACCATCGTGATGAAGAAGACAACTCCCCCCCTGACATCACTGACAACATGCTCAAACTTCTCGGGAAACGTAGAAGATCAAAACGGCAGTCTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001047 GO:0001067 GO:0001503 GO:0001817 GO:0001819 GO:0002151 GO:0002791 GO:0002793 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003697 GO:0003723 GO:0003724 GO:0003725 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006139 GO:0006355 GO:0006357 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0008186 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010501 GO:0010556 GO:0010557 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010638 GO:0014070 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019219 GO:0019222 GO:0019899 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0032204 GO:0032206 GO:0032479 GO:0032481 GO:0032501 GO:0032647 GO:0032727 GO:0032879 GO:0032880 GO:0033043 GO:0033044 GO:0034641 GO:0042221 GO:0042623 GO:0042826 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043330 GO:0043331 GO:0043487 GO:0043489 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050707 GO:0050708 GO:0050714 GO:0050715 GO:0050789 GO:0050794 GO:0050896 GO:0051046 GO:0051047 GO:0051049 GO:0051050 GO:0051052 GO:0051054 GO:0051128 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051222 GO:0051223 GO:0051239 GO:0051240 GO:0051252 GO:0051253 GO:0051254 GO:0051704 GO:0051707 GO:0051880 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070035 GO:0070201 GO:0071704 GO:0080090 GO:0090087 GO:0090304 GO:0090669 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1901698 GO:1902369 GO:1902680 GO:1902739 GO:1902741 GO:1903506 GO:1903508 GO:1903530 GO:1903532 GO:1904951 GO:1990837 GO:2000112 GO:2001141 GO:2001252
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
DEAD HA2 Helicase_C OB_NTP_bind dsrm

Protein Analysis

1157

Amino Acids

130.02

Weight (kDa)

8.8

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DEAD PF00270 266 - 420 1.1e-08 DEAD/DEAH box helicase
Helicase_C PF00271 527 - 656 8.4e-10 Helicase conserved C-terminal domain
WHD_HA2 PF04408 720 - 748 3.1e-07 Helicase associated domain (HA2), winged-helix
HA2_C PF21010 749 - 809 5.9e-15 Helicase associated domain (HA2), ratchet-like
OB_NTP_bind PF07717 876 - 957 1.9e-16 Oligonucleotide/oligosaccharide-binding (OB)-fold
RNA_hel_CTD PF26026 966 - 1024 9.7e-23 C-terminal domain in RNA helicases
dsrm PF00035 1057 - 1119 7.8e-07 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2692
AatII GACGTC 1 cut(s) 2690
Acc16I TGCGCA 1 cut(s) 707
Acc36I ACCTGC 1 cut(s) 2080
AccB1I GGYRCC 1 cut(s) 1435
AccB7I CCANNNNNTGG 1 cut(s) 2623
AccII CGCG 2 cut(s) 180, 227
AccIII TCCGGA 1 cut(s) 3071
AcoI YGGCCR 6 cut(s) 429, 479, 907, 1399, 1960, 2716
AcsI RAATTY 5 cut(s) 233, 1149, 2108, 2203, 2494
AcuI CTGAAG 3 cut(s) 243, 1214, 2501
AcvI CACGTG 1 cut(s) 1523
AcyI GRCGYC 1 cut(s) 2687
AfaI GTAC 5 cut(s) 194, 224, 1075, 1347, 3041
AfiI CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
AflII CTTAAG 2 cut(s) 2966, 3239
AflIII ACRYGT 1 cut(s) 225
AjnI CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
Alw21I GWGCWC 6 cut(s) 546, 606, 1288, 1330, 2679, 3262
Alw26I GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
Alw44I GTGCAC 1 cut(s) 2675
Ama87I CYCGRG 1 cut(s) 3434
Aor13HI TCCGGA 1 cut(s) 3071
AoxI GGCC 9 cut(s) 241, 429, 458, 479, 907, 1399, 1960, 2716, 3348
ApaLI GTGCAC 1 cut(s) 2675
ApoI RAATTY 5 cut(s) 233, 1149, 2108, 2203, 2494
ArsI GACNNNNNNTTYG 1 cut(s) 3446
AseI ATTAAT 1 cut(s) 2595
Asp700I GAANNNNTTC 1 cut(s) 3251
AspLEI GCGC 3 cut(s) 180, 182, 708
AspS9I GGNCC 3 cut(s) 319, 439, 2884
AsuC2I CCSGG 2 cut(s) 1305, 1964
AsuHPI GGTGA 5 cut(s) 726, 1019, 1703, 2801, 3146
AsuII TTCGAA 1 cut(s) 237
AvaI CYCGRG 1 cut(s) 3434
AvaII GGWCC 3 cut(s) 319, 439, 2884
BaeGI GKGCMC 2 cut(s) 1522, 2679
BaeI ACNNNNGTAYC 2 cut(s) 2820, 2853
BalI TGGCCA 3 cut(s) 431, 1401, 2718
BamHI GGATCC 3 cut(s) 2245, 2335, 2935
BanI GGYRCC 1 cut(s) 1435
BanII GRGCYC 2 cut(s) 160, 1288
BbrPI CACGTG 1 cut(s) 1523
BbsI GAAGAC 3 cut(s) 249, 1771, 3396
Bbv12I GWGCWC 6 cut(s) 546, 606, 1288, 1330, 2679, 3262
BccI CCATC 8 cut(s) 385, 653, 1068, 2439, 2701, 2708, 2906, 3384
BceAI ACGGC 4 cut(s) 166, 894, 1080, 1197
BcgI CGANNNNNNTGC 2 cut(s) 533, 567
BciT130I CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
BciVI GTATCC 1 cut(s) 343
BclI TGATCA 1 cut(s) 2413
BcnI CCSGG 2 cut(s) 1305, 1964
BcoDI GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
BfaI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
BfmI CTRYAG 3 cut(s) 454, 922, 3336
BfrI CTTAAG 2 cut(s) 2966, 3239
BfuAI ACCTGC 1 cut(s) 2080
BfuI GTATCC 1 cut(s) 343
BlpI GCTNAGC 1 cut(s) 1257
Bme18I GGWCC 3 cut(s) 319, 439, 2884
BmeT110I CYCGRG 1 cut(s) 3434
BmgT120I GGNCC 3 cut(s) 319, 439, 2884
BmiI GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
BmrI ACTGGG 3 cut(s) 1368, 1644, 2264
BmuI ACTGGG 3 cut(s) 1368, 1644, 2264
BpiI GAAGAC 3 cut(s) 249, 1771, 3396
BplI GAGNNNNNCTC 4 cut(s) 194, 226, 1231, 1263
BpmI CTGGAG 2 cut(s) 542, 2509
Bpu1102I GCTNAGC 1 cut(s) 1257
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 2 cut(s) 20, 2337
BpuMI CCSGG 2 cut(s) 1305, 1964
BsaAI YACGTR 2 cut(s) 1523, 2822
BsaBI GATNNNNATC 1 cut(s) 2852
BsaHI GRCGYC 1 cut(s) 2687
BsaI GGTCTC 2 cut(s) 32, 1865
BsaJI CCNNGG 7 cut(s) 910, 1515, 1723, 2622, 2656, 2774, 2878
BsaWI WCCGGW 2 cut(s) 2134, 3071
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
Bse118I RCCGGY 1 cut(s) 775
Bse3DI GCAATG 1 cut(s) 2287
Bse8I GATNNNNATC 1 cut(s) 2852
BseAI TCCGGA 1 cut(s) 3071
BseBI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
BseDI CCNNGG 7 cut(s) 910, 1515, 1723, 2622, 2656, 2774, 2878
BseJI GATNNNNATC 1 cut(s) 2852
BseLI CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
BseMI GCAATG 1 cut(s) 2287
BseMII CTCAG 4 cut(s) 591, 1248, 2059, 3381
BsePI GCGCGC 1 cut(s) 178
BseRI GAGGAG 3 cut(s) 706, 1296, 3204
BseSI GKGCMC 2 cut(s) 1522, 2679
BseYI CCCAGC 1 cut(s) 2891
Bsh1236I CGCG 2 cut(s) 180, 227
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
BshNI GGYRCC 1 cut(s) 1435
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 6 cut(s) 546, 606, 1288, 1330, 2679, 3262
BsiHKCI CYCGRG 1 cut(s) 3434
BsiSI CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
BslFI GGGAC 4 cut(s) 408, 425, 608, 2389
BslI CCNNNNNNNGG 7 cut(s) 189, 990, 1688, 2255, 2256, 2623, 2779
BsmAI GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
BsmBI CGTCTC 1 cut(s) 2678
BsmFI GGGAC 4 cut(s) 408, 425, 608, 2389
BsmI GAATGC 4 cut(s) 646, 708, 917, 1258
BsnI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
Bso31I GGTCTC 2 cut(s) 32, 1865
BsoBI CYCGRG 1 cut(s) 3434
Bsp119I TTCGAA 1 cut(s) 237
Bsp1286I GDGCHC 8 cut(s) 160, 546, 606, 1288, 1330, 1522, 2679, 3262
Bsp13I TCCGGA 1 cut(s) 3071
Bsp1407I TGTACA 1 cut(s) 1073
Bsp1720I GCTNAGC 1 cut(s) 1257
Bsp19I CCATGG 2 cut(s) 1723, 2878
BspANI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
BspCNI CTCAG 4 cut(s) 592, 1249, 2058, 3380
BspEI TCCGGA 1 cut(s) 3071
BspFNI CGCG 2 cut(s) 180, 227
BspHI TCATGA 2 cut(s) 1153, 1629
BspLI GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
BspMI ACCTGC 1 cut(s) 2080
BspT104I TTCGAA 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 1435
BspTI CTTAAG 2 cut(s) 2966, 3239
BspTNI GGTCTC 2 cut(s) 32, 1865
BsrDI GCAATG 1 cut(s) 2287
BsrFI RCCGGY 1 cut(s) 775
BsrGI TGTACA 1 cut(s) 1073
BssAI RCCGGY 1 cut(s) 775
BssECI CCNNGG 7 cut(s) 910, 1515, 1723, 2622, 2656, 2774, 2878
BssHII GCGCGC 1 cut(s) 178
BssNI GRCGYC 1 cut(s) 2687
BssT1I CCWWGG 3 cut(s) 1723, 2622, 2878
Bst2UI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
Bst4CI ACNGT 7 cut(s) 92, 1015, 1413, 1511, 2411, 2812, 3143
Bst6I CTCTTC 2 cut(s) 450, 2538
BstACI GRCGYC 1 cut(s) 2687
BstAFI CTTAAG 2 cut(s) 2966, 3239
BstAPI GCANNNNNTGC 2 cut(s) 933, 1435
BstAUI TGTACA 1 cut(s) 1073
BstBAI YACGTR 2 cut(s) 1523, 2822
BstBI TTCGAA 1 cut(s) 237
BstDEI CTNAG 8 cut(s) 600, 1257, 2045, 2324, 2606, 3171, 3300, 3367
BstDSI CCRYGG 3 cut(s) 910, 1723, 2878
BstFNI CGCG 2 cut(s) 180, 227
BstHHI GCGC 3 cut(s) 180, 182, 708
BstMAI GTCTC 7 cut(s) 32, 49, 351, 839, 1865, 2219, 2678
BstMCI CGRYCG 1 cut(s) 553
BstMWI GCNNNNNNNGC 8 cut(s) 186, 428, 541, 712, 933, 1435, 2378, 3341
BstNI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
BstNSI RCATGY 3 cut(s) 1722, 3283, 3424
BstSFI CTRYAG 3 cut(s) 454, 922, 3336
BstSLI GKGCMC 2 cut(s) 1522, 2679
BstUI CGCG 2 cut(s) 180, 227
BstV2I GAAGAC 3 cut(s) 249, 1771, 3396
BstX2I RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
BstXI CCANNNNNNTGG 1 cut(s) 391
BstYI RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
BsuI GTATCC 1 cut(s) 343
BsuRI GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
BtgI CCRYGG 3 cut(s) 910, 1723, 2878
BtsIMutI CAGTG 9 cut(s) 267, 579, 1516, 2247, 2416, 2829, 3260, 3344, 3411
BveI ACCTGC 1 cut(s) 2080
CciI TCATGA 2 cut(s) 1153, 1629
CfoI GCGC 3 cut(s) 180, 182, 708
Cfr10I RCCGGY 1 cut(s) 775
Cfr13I GGNCC 3 cut(s) 319, 439, 2884
Csp6I GTAC 5 cut(s) 193, 223, 1074, 1346, 3040
CspCI CAANNNNNGTGG 2 cut(s) 492, 527
CviQI GTAC 5 cut(s) 193, 223, 1074, 1346, 3040
DdeI CTNAG 8 cut(s) 600, 1257, 2045, 2324, 2606, 3171, 3300, 3367
DrdI GACNNNNNNGTC 1 cut(s) 2692
DseDI GACNNNNNNGTC 1 cut(s) 2692
EaeI YGGCCR 6 cut(s) 429, 479, 907, 1399, 1960, 2716
Eam1104I CTCTTC 2 cut(s) 450, 2538
EarI CTCTTC 2 cut(s) 450, 2538
EciI GGCGGA 1 cut(s) 168
Ecl136II GAGCTC 1 cut(s) 1286
Eco130I CCWWGG 3 cut(s) 1723, 2622, 2878
Eco147I AGGCCT 1 cut(s) 460
Eco24I GRGCYC 2 cut(s) 160, 1288
Eco31I GGTCTC 2 cut(s) 32, 1865
Eco32I GATATC 4 cut(s) 372, 837, 1923, 3022
Eco47I GGWCC 3 cut(s) 319, 439, 2884
Eco53kI GAGCTC 1 cut(s) 1286
Eco57I CTGAAG 3 cut(s) 243, 1214, 2501
Eco72I CACGTG 1 cut(s) 1523
Eco88I CYCGRG 1 cut(s) 3434
EcoICRI GAGCTC 1 cut(s) 1286
EcoO109I RGGNCCY 1 cut(s) 439
EcoRI GAATTC 2 cut(s) 233, 2108
EcoRII CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
EcoRV GATATC 4 cut(s) 372, 837, 1923, 3022
EcoT14I CCWWGG 3 cut(s) 1723, 2622, 2878
EcoT38I GRGCYC 2 cut(s) 160, 1288
ErhI CCWWGG 3 cut(s) 1723, 2622, 2878
Esp3I CGTCTC 1 cut(s) 2678
FalI AAGNNNNNCTT 2 cut(s) 167, 199
FaqI GGGAC 4 cut(s) 408, 425, 608, 2389
FauI CCCGC 2 cut(s) 86, 182
FauNDI CATATG 4 cut(s) 2418, 2437, 2476, 2639
FbaI TGATCA 1 cut(s) 2413
FriOI GRGCYC 2 cut(s) 160, 1288
FspBI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
FspI TGCGCA 1 cut(s) 707
GlaI GCGC 3 cut(s) 179, 181, 707
GsaI CCCAGC 1 cut(s) 2895
GsuI CTGGAG 2 cut(s) 542, 2509
HaeIII GGCC 9 cut(s) 243, 431, 460, 481, 909, 1401, 1962, 2718, 3350
HapII CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
HhaI GCGC 3 cut(s) 180, 182, 708
Hin1I GRCGYC 1 cut(s) 2687
Hin6I GCGC 3 cut(s) 178, 180, 706
HinP1I GCGC 3 cut(s) 178, 180, 706
HincII GTYRAC 2 cut(s) 1371, 3357
HindII GTYRAC 2 cut(s) 1371, 3357
HindIII AAGCTT 3 cut(s) 1495, 2963, 2999
HpaII CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
HphI GGTGA 5 cut(s) 726, 1019, 1703, 2801, 3146
Hpy166II GTNNAC 5 cut(s) 579, 1371, 2677, 2950, 3357
Hpy8I GTNNAC 5 cut(s) 579, 1371, 2677, 2950, 3357
Hpy99I CGWCG 3 cut(s) 152, 218, 1218
HpyCH4III ACNGT 7 cut(s) 92, 1015, 1413, 1511, 2411, 2812, 3143
HpyCH4IV ACGT 4 cut(s) 1522, 2687, 2821, 3442
HpyF10VI GCNNNNNNNGC 8 cut(s) 186, 428, 541, 712, 933, 1435, 2378, 3341
HpyF3I CTNAG 8 cut(s) 600, 1257, 2045, 2324, 2606, 3171, 3300, 3367
HpySE526I ACGT 4 cut(s) 1522, 2687, 2821, 3442
Hsp92I GRCGYC 1 cut(s) 2687
HspAI GCGC 3 cut(s) 178, 180, 706
Kpn2I TCCGGA 1 cut(s) 3071
Ksp22I TGATCA 1 cut(s) 2413
LmnI GCTCC 4 cut(s) 155, 914, 1283, 1291
MaeI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
MaeII ACGT 4 cut(s) 1522, 2687, 2821, 3442
MaeIII GTNAC 6 cut(s) 1112, 1505, 1982, 2609, 2753, 3107
MfeI CAATTG 2 cut(s) 810, 2178
MflI RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
MhlI GDGCHC 8 cut(s) 160, 546, 606, 1288, 1330, 1522, 2679, 3262
MlsI TGGCCA 3 cut(s) 431, 1401, 2718
MluI ACGCGT 1 cut(s) 225
MluNI TGGCCA 3 cut(s) 431, 1401, 2718
MlyI GAGTC 4 cut(s) 549, 590, 899, 3352
MmeI TCCRAC 7 cut(s) 72, 145, 171, 237, 1520, 1577, 2889
Mox20I TGGCCA 3 cut(s) 431, 1401, 2718
MroI TCCGGA 1 cut(s) 3071
MroXI GAANNNNTTC 1 cut(s) 3251
MscI TGGCCA 3 cut(s) 431, 1401, 2718
MslI CAYNNNNRTG 2 cut(s) 1534, 3381
Msp20I TGGCCA 3 cut(s) 431, 1401, 2718
MspA1I CMGCKG 1 cut(s) 2120
MspCI CTTAAG 2 cut(s) 2966, 3239
MspI CCGG 5 cut(s) 776, 1304, 1963, 2135, 3072
MunI CAATTG 2 cut(s) 810, 2178
Mva1269I GAATGC 4 cut(s) 646, 708, 917, 1258
MvaI CCWGG 8 cut(s) 632, 1516, 1974, 2658, 2720, 2776, 2888, 3352
MvnI CGCG 2 cut(s) 180, 227
MwoI GCNNNNNNNGC 8 cut(s) 186, 428, 541, 712, 933, 1435, 2378, 3341
NciI CCSGG 2 cut(s) 1305, 1964
NcoI CCATGG 2 cut(s) 1723, 2878
NdeI CATATG 4 cut(s) 2418, 2437, 2476, 2639
NlaIV GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
NmeAIII GCCGAG 2 cut(s) 226, 1918
NmuCI GTSAC 2 cut(s) 2609, 3107
NsbI TGCGCA 1 cut(s) 707
NspI RCATGY 3 cut(s) 1722, 3283, 3424
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 3283
PagI TCATGA 2 cut(s) 1153, 1629
PauI GCGCGC 1 cut(s) 178
PceI AGGCCT 1 cut(s) 460
PctI GAATGC 4 cut(s) 646, 708, 917, 1258
PdmI GAANNNNTTC 1 cut(s) 3251
PflMI CCANNNNNTGG 1 cut(s) 2623
Ple19I CGATCG 1 cut(s) 553
PleI GAGTC 4 cut(s) 549, 590, 898, 3352
PmaCI CACGTG 1 cut(s) 1523
PmlI CACGTG 1 cut(s) 1523
PpsI GAGTC 4 cut(s) 549, 590, 898, 3352
Ppu21I YACGTR 2 cut(s) 1523, 2822
PpuMI RGGWCCY 1 cut(s) 439
PshBI ATTAAT 1 cut(s) 2595
Psp124BI GAGCTC 1 cut(s) 1288
Psp5II RGGWCCY 1 cut(s) 439
Psp6I CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
PspCI CACGTG 1 cut(s) 1523
PspFI CCCAGC 1 cut(s) 2891
PspGI CCWGG 8 cut(s) 630, 1514, 1972, 2656, 2718, 2774, 2886, 3350
PspN4I GGNNCC 9 cut(s) 157, 441, 1437, 2139, 2247, 2337, 2885, 2937, 3163
PspPI GGNCC 3 cut(s) 319, 439, 2884
PspPPI RGGWCCY 1 cut(s) 439
PsuI RGATCY 5 cut(s) 1198, 2245, 2335, 2935, 3007
PteI GCGCGC 1 cut(s) 178
PvuI CGATCG 1 cut(s) 553
PvuII CAGCTG 1 cut(s) 2120
RsaI GTAC 5 cut(s) 194, 224, 1075, 1347, 3041
RsaNI GTAC 5 cut(s) 193, 223, 1074, 1346, 3040
RseI CAYNNNNRTG 2 cut(s) 1534, 3381
SacI GAGCTC 1 cut(s) 1288
Sau96I GGNCC 3 cut(s) 319, 439, 2884
SchI GAGTC 4 cut(s) 549, 590, 899, 3352
SduI GDGCHC 8 cut(s) 160, 546, 606, 1288, 1330, 1522, 2679, 3262
SfcI CTRYAG 3 cut(s) 454, 922, 3336
SfuI TTCGAA 1 cut(s) 237
SinI GGWCC 3 cut(s) 319, 439, 2884
SmiMI CAYNNNNRTG 2 cut(s) 1534, 3381
SmlI CTYRAG 4 cut(s) 35, 2352, 2966, 3239
SmoI CTYRAG 4 cut(s) 35, 2352, 2966, 3239
SphI GCATGC 1 cut(s) 3283
SseBI AGGCCT 1 cut(s) 460
SspI AATATT 2 cut(s) 2225, 2483
SspMI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
SstI GAGCTC 1 cut(s) 1288
StuI AGGCCT 1 cut(s) 460
StyI CCWWGG 3 cut(s) 1723, 2622, 2878
TaaI ACNGT 7 cut(s) 92, 1015, 1413, 1511, 2411, 2812, 3143
TaiI ACGT 4 cut(s) 1525, 2690, 2824, 3445
TatI WGTACW 2 cut(s) 1073, 1345
TauI GCSGC 3 cut(s) 102, 200, 481
TscAI CASTG 9 cut(s) 267, 586, 1516, 2247, 2416, 2836, 3267, 3351, 3418
TseFI GTSAC 2 cut(s) 2609, 3107
Tsp45I GTSAC 2 cut(s) 2609, 3107
TspGWI ACGGA 1 cut(s) 3174
TspRI CASTG 9 cut(s) 267, 586, 1516, 2247, 2416, 2836, 3267, 3351, 3418
Van91I CCANNNNNTGG 1 cut(s) 2623
Vha464I CTTAAG 2 cut(s) 2966, 3239
VneI GTGCAC 1 cut(s) 2675
VpaK11BI GGWCC 3 cut(s) 319, 439, 2884
VspI ATTAAT 1 cut(s) 2595
XapI RAATTY 5 cut(s) 233, 1149, 2108, 2203, 2494
XbaI TCTAGA 2 cut(s) 655, 2387
XceI RCATGY 3 cut(s) 1722, 3283, 3424
XcmI CCANNNNNNNNNTGG 2 cut(s) 566, 2159
XmnI GAANNNNTTC 1 cut(s) 3251
XspI CTAG 8 cut(s) 656, 755, 887, 2097, 2144, 2388, 2940, 3012
ZraI GACGTC 1 cut(s) 2688
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.