pycom04g14630
ERF Family

Gamma interferon inducible lysosomal thiol reductase (GILT)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005189
Physical Location & Seq
Reverse (-)
17487815 .. 17488806
992 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 678 bp
ATGGCTTCTCCTAAGCTCTTCTCCCTTCTCATTCTAACTTCTTTGCTCATCATTTGCGTGTCTTGTTCTTCTTTTGAAGATCAAGGCAGCAAAGTCCCACCTTCTCCAAAATCCCGAAAACTTATAGAAGCTCCTAGTGCTATTCCTACGCCTCCTACCAAAGCTAAGAAAGTTGACCTGACTCTGTATTACGAAACACTATGCCCATTCTGTGCACAGTTCATCGTAAATGGACTATCAAAGGTATTCAACACAGATCTCATCAACATTGTGAACTTGAGGCTAGTCCCATATGGAAATGCTCATATACAAGGACCTAACAGCACCATCGTTTGCCAGCACGGCCCGGATGAGTGCTACTTGAACAGCATAGAAGCCTGTGCTATCAACGGCTACCCAGATGTGAGTTTTCACTTGATCTTCATCGTCACTTTAACTCTGATGCAGACGCAGCATTTCAAATTCATTCATTGCATTGAGAACCAAACTATTCAAGGAAAGCAGTCCAAAGATGAATGGAAATCTTGTTCTCAACAACTAGGGATGGATCCAAAACCTGTCCAAGATTGCTACGACAGTGGGTTAGAGAAAAAGGTTCCCACTAATTTGTTAAACAGCATTTTGTTGAAACTCATGTATACAAGTCCAAACAATATTTTCATTCAACAAACAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04612 map04612
Pfam Domains
Protein Families
GILT

Protein Analysis

226

Amino Acids

25.07

Weight (kDa)

8.0

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GILT PF03227 60 - 179 4.1e-27 Gamma interferon inducible lysosomal thiol reductase (GILT)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 638
AclWI GGATC 2 cut(s) 542, 555
AcsI RAATTY 1 cut(s) 461
AgsI TTSAA 7 cut(s) 77, 250, 364, 460, 494, 628, 665
AloI GAACNNNNNNTCC 2 cut(s) 511, 543
AluBI AGCT 3 cut(s) 16, 131, 164
AluI AGCT 3 cut(s) 16, 131, 164
Alw21I GWGCWC 1 cut(s) 217
Alw44I GTGCAC 1 cut(s) 213
AlwI GGATC 2 cut(s) 542, 555
AoxI GGCC 1 cut(s) 343
ApaLI GTGCAC 1 cut(s) 213
ApeKI GCWGC 2 cut(s) 87, 451
ApoI RAATTY 1 cut(s) 461
AspS9I GGNCC 2 cut(s) 314, 344
AsuC2I CCSGG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 314
BaeGI GKGCMC 1 cut(s) 217
BamHI GGATCC 1 cut(s) 547
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 2 cut(s) 99, 463
BccI CCATC 2 cut(s) 335, 538
BceAI ACGGC 2 cut(s) 358, 406
BcnI CCSGG 1 cut(s) 347
BfaI CTAG 3 cut(s) 135, 284, 539
BglI GCCNNNNNGGC 1 cut(s) 342
BglII AGATCT 1 cut(s) 256
BisI GCNGC 2 cut(s) 88, 452
BlsI GCNGC 2 cut(s) 89, 453
Bme1390I CCNGG 1 cut(s) 347
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 314, 344
BmiI GGNNCC 2 cut(s) 549, 597
BmrFI CCNGG 1 cut(s) 347
BmsI GCATC 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 298
BpuMI CCSGG 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 422
Bse3DI GCAATG 1 cut(s) 469
Bse8I GATNNNNATC 1 cut(s) 422
BseGI GGATG 2 cut(s) 355, 549
BseJI GATNNNNATC 1 cut(s) 422
BseMI GCAATG 1 cut(s) 469
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 99, 463
BshFI GGCC 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 347
BslFI GGGAC 2 cut(s) 80, 272
BsmFI GGGAC 2 cut(s) 80, 272
BsnI GGCC 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 4 cut(s) 79, 256, 417, 547
BspANI GGCC 1 cut(s) 345
BspLI GGNNCC 2 cut(s) 549, 597
BspPI GGATC 2 cut(s) 542, 555
BspQI GCTCTTC 1 cut(s) 23
BsrDI GCAATG 1 cut(s) 469
BssMI GATC 4 cut(s) 79, 256, 417, 547
BssNAI GTATAC 1 cut(s) 639
Bst1107I GTATAC 1 cut(s) 639
Bst4CI ACNGT 2 cut(s) 219, 578
Bst6I CTCTTC 1 cut(s) 23
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 12, 165
BstF5I GGATG 2 cut(s) 355, 549
BstKTI GATC 4 cut(s) 82, 259, 420, 550
BstMBI GATC 4 cut(s) 79, 256, 417, 547
BstMWI GCNNNNNNNGC 3 cut(s) 137, 342, 451
BstSCI CCNGG 1 cut(s) 345
BstSLI GKGCMC 1 cut(s) 217
BstV1I GCAGC 2 cut(s) 99, 463
BstX2I RGATCY 2 cut(s) 256, 547
BstYI RGATCY 2 cut(s) 256, 547
BstZ17I GTATAC 1 cut(s) 639
BsuRI GGCC 1 cut(s) 345
BtsCI GGATG 2 cut(s) 355, 549
BtsIMutI CAGTG 1 cut(s) 583
Cac8I GCNNGC 1 cut(s) 338
Cfr13I GGNCC 2 cut(s) 314, 344
CseI GACGC 1 cut(s) 457
CviAII CATG 1 cut(s) 634
CviJI RGCY 8 cut(s) 5, 16, 131, 164, 283, 345, 377, 393
CviKI_1 RGCY 8 cut(s) 5, 16, 131, 164, 283, 345, 377, 393
DdeI CTNAG 2 cut(s) 12, 165
DpnI GATC 4 cut(s) 81, 258, 419, 549
DpnII GATC 4 cut(s) 79, 256, 417, 547
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco47I GGWCC 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 314
FaeI CATG 1 cut(s) 637
FaiI YATR 9 cut(s) 125, 202, 292, 294, 306, 308, 371, 635, 639
FaqI GGGAC 2 cut(s) 80, 272
FatI CATG 1 cut(s) 633
FauNDI CATATG 1 cut(s) 292
FblI GTMKAC 1 cut(s) 638
Fnu4HI GCNGC 2 cut(s) 88, 452
FokI GGATG 2 cut(s) 362, 556
Fsp4HI GCNGC 2 cut(s) 88, 452
FspBI CTAG 3 cut(s) 135, 284, 539
GluI GCNGC 2 cut(s) 88, 452
HaeIII GGCC 1 cut(s) 345
HapII CCGG 1 cut(s) 347
HgaI GACGC 1 cut(s) 457
Hin1II CATG 1 cut(s) 637
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 1 cut(s) 181
HpaII CCGG 1 cut(s) 347
Hpy166II GTNNAC 4 cut(s) 175, 215, 274, 639
Hpy188I TCNGA 1 cut(s) 441
Hpy188III TCNNGA 1 cut(s) 114
Hpy8I GTNNAC 4 cut(s) 175, 215, 274, 639
HpyAV CCTTC 2 cut(s) 35, 111
HpyCH4III ACNGT 2 cut(s) 219, 578
HpyCH4V TGCA 3 cut(s) 215, 445, 474
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 342, 451
HpyF3I CTNAG 2 cut(s) 12, 165
Hsp92II CATG 1 cut(s) 637
Kzo9I GATC 4 cut(s) 79, 256, 417, 547
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 1 cut(s) 136
LpnPI CCDG 6 cut(s) 191, 350, 360, 391, 411, 570
Lsp1109I GCAGC 2 cut(s) 99, 463
LweI GCATC 1 cut(s) 432
MaeI CTAG 3 cut(s) 135, 284, 539
MaeIII GTNAC 1 cut(s) 427
MalI GATC 4 cut(s) 81, 258, 419, 549
MboI GATC 4 cut(s) 79, 256, 417, 547
MboII GAAGA 4 cut(s) 10, 60, 89, 412
MflI RGATCY 2 cut(s) 256, 547
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 461, 604
MlyI GAGTC 1 cut(s) 175
MnlI CCTC 2 cut(s) 162, 273
MseI TTAA 2 cut(s) 434, 611
MslI CAYNNNNRTG 1 cut(s) 56
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 347
MwoI GCNNNNNNNGC 3 cut(s) 137, 342, 451
NciI CCSGG 1 cut(s) 347
NdeI CATATG 1 cut(s) 292
NdeII GATC 4 cut(s) 79, 256, 417, 547
NlaIII CATG 1 cut(s) 637
NlaIV GGNNCC 2 cut(s) 549, 597
NmuCI GTSAC 1 cut(s) 427
PciSI GCTCTTC 1 cut(s) 23
PkrI GCNGC 2 cut(s) 89, 453
PleI GAGTC 1 cut(s) 175
PpsI GAGTC 1 cut(s) 175
PpuMI RGGWCCY 1 cut(s) 314
Psp5II RGGWCCY 1 cut(s) 314
PspN4I GGNNCC 2 cut(s) 549, 597
PspPI GGNCC 2 cut(s) 314, 344
PspPPI RGGWCCY 1 cut(s) 314
PsuI RGATCY 2 cut(s) 256, 547
RseI CAYNNNNRTG 1 cut(s) 56
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 434, 611
SatI GCNGC 2 cut(s) 88, 452
Sau3AI GATC 4 cut(s) 79, 256, 417, 547
Sau96I GGNCC 2 cut(s) 314, 344
SchI GAGTC 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 347
SduI GDGCHC 1 cut(s) 217
SetI ASST 9 cut(s) 18, 103, 133, 166, 180, 246, 319, 559, 597
SfaNI GCATC 1 cut(s) 432
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 56
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
Sse9I AATT 2 cut(s) 461, 604
SspI AATATT 1 cut(s) 655
SspMI CTAG 3 cut(s) 135, 284, 539
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 219, 578
TasI AATT 2 cut(s) 461, 604
Tru1I TTAA 2 cut(s) 434, 611
Tru9I TTAA 2 cut(s) 434, 611
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 2 cut(s) 87, 451
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 6 cut(s) 211, 412, 454, 458, 528, 649
TspRI CASTG 1 cut(s) 583
VneI GTGCAC 1 cut(s) 213
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 638
XspI CTAG 3 cut(s) 135, 284, 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.