Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0000765

Physical Location & Seq

Genomic Coordinates Forward (+)

20845997 .. 20848653

Gene Structure 2657 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g19030.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGAGATGGCTGACCAGAGAGCAGAAAATAACGTCGTCTCCGGCCAACCCCAGAATAGTATTGCTGACTTTGATCCCCCAAAGAAACCCAAGACAAAGAAGTTTGCTGTTGCCTGTGCCGTTTTGGCTTCAATGACGTCTATCTTACTGGGTTATGATATTGGTGTAATGAGTGGAGCGTCTCTCTTCATCAAAGAAAACCTCAATATCAGCGATGTTCAAGTTGAAGTTCTTAACGGTACCTTGAATATATACTCTCTCATTGGCTCCGCCTTGGCCGGTAAAACCTCTGACTGGATTGGACGCCGGTACACCATAGTACTTGCCGGAACAATCTTCTTTATTGGAGCTCTCCTCATGGGTTTTGCCCCGAACTACGCCTTCCTCATGTTTGGTAGATTCATTGCCGGAGTTGGAGTTGGCTATGCTCTTATGATAGCTCCTGTCTACACCGCCGAGATCTCTCCGGCCTCGTTTCGTGGCTTCCTCACATCTTTCCCTGAGGTGTTTGTTAACGTTGGTATATTACTGGGGTACGTATCCAACTTTGCCTTAGCCAAGCTCCCAATCCACTTGAACTGGAGGATCATGCTCGGCATCGGCGCAATTCCCTCAGTTTTTCTAGCCCTCGGCGTTTTAGCCATGCCCGAGTCACCACGCTGGCTCGTCATGCAAGGGAGACTCGGAGACGCCAAGCGAGTACTAAATAGAACATCATCTTCTGAAGAGGAATCGCAGCTCAGACTAGACGAGATTAAAGAAGCTGCAGGAATCCCCAAAGACTCAAAGGATGAGGTCGTTCAGGTTTCTAAACGCAGCCAAGGTGAAGGTATATGGAAGGAATTGCTCATTCGCCCTACGCCGGCCGTTCGCCACATTTTAATAGCAGCCCTTGGTATCCACTTCTTCCAACAAGTGTCGGGTACAGACTCTGTGGTTTTGTACAGCCCCAGGATCTTCGAGAAAGCGGGAATCACTTCTTACAATGACAAGCTACTTGCAACCGTGGCTGTTGGATTTGTCAAGACCATTGCAATCTTGGTGGCAACATTTCAAGTTGATCGGTTCGGACGTCGTGCTTTGCTTTTAAGCAGCGTGGGTGGAATGGTAGTTTCTTTTACGCTACTCGGTGTGGGTCTTACGGTCGTTGATCAATCCCACAGCAAGGTCCCATGGGCCGTCGGGTTGTGCATCGCCATGGTACTATTCATAGTCGCTTTTTTCTCCATTGGGTTGGGACCCATCACGTGGGTCTACAGCTCTGAGATCTTTCCGTTGCAGTTGCGCGCACAAGGGTGCAGTATGGGGGTGGCCGTAAACAGGTTGACGAGCGGGGTCATCTCCATGACTTTTATTTCATTGTATAAGGCCATCACGATTGGTGGGGCCTTCTTTCTTTACGCGGGAATTGCTGCGCTTAGTTGGGTCTTCTTTTATACGTTGTATCCAGAAACGCAGGGTCGATCCCTTGAAGATATGGAGGTCTTGTTTGGTAAATACCACAAGTGGAGAGAAGCCAATGCCATGCTTAACAAGACTAAGCAAGCTGATCATGGTTTTGGTGACGTCAACAAGGCTCAAATCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005351 GO:0005354 GO:0005355 GO:0005365 GO:0005402 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0007275 GO:0008150 GO:0008324 GO:0008643 GO:0008645 GO:0009653 GO:0009791 GO:0010015 GO:0010101 GO:0010102 GO:0010311 GO:0015075 GO:0015077 GO:0015078 GO:0015144 GO:0015145 GO:0015146 GO:0015148 GO:0015149 GO:0015166 GO:0015168 GO:0015291 GO:0015293 GO:0015294 GO:0015295 GO:0015318 GO:0015575 GO:0015576 GO:0015591 GO:0015672 GO:0015749 GO:0015750 GO:0015752 GO:0015753 GO:0015757 GO:0015791 GO:0015793 GO:0015795 GO:0015797 GO:0015798 GO:0015850 GO:0016020 GO:0022622 GO:0022804 GO:0022857 GO:0022890 GO:0032501 GO:0032502 GO:0034219 GO:0034220 GO:0042995 GO:0044464 GO:0046323 GO:0048364 GO:0048527 GO:0048528 GO:0048646 GO:0048731 GO:0048856 GO:0051119 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071944 GO:0090406 GO:0090696 GO:0090697 GO:0090698 GO:0098655 GO:0098660 GO:0098662 GO:0099402 GO:0120025 GO:1901618 GO:1902600 GO:1904659 GO:1905392 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

530

Amino Acids

57.54

Weight (kDa)

9.18

Isoelectric Point (pI)

36.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	39 - 497	2.6e-108	Sugar (and other) transporter
MFS_1	PF07690	46 - 330	1.3e-21	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000765)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16120 AT2G16130 AT3G18830
fragaria_vesca	FvH4_2g08400 FvH4_2g08400 FvH4_2g08430 FvH4_2g08430 FvH4_2g08430
malus_domestica	MD05G1068600.v1.1 MD05G1068700.v1.1 MD05G1069100.v1.1 MD05G1069700.v1.1 MD10G1078900.v1.1 MD10G1079100.v1.1 MD10G1079200.v1.1 MD10G1079500.v1.1 MD10G1079600.v1.1 MD10G1079700.v1.1 MD10G1079800.v1.1 MD10G1079900.v1.1 MD10G1080000.v1.1 MD10G1080100.v1.1 MD10G1106100.v1.1 MD12G1026200.v1.1 MD17G1247200.v1.1
prunus_persica	Prupe.3G071100_v2.0.a1 Prupe.8G100700_v2.0.a1 Prupe.8G101000_v2.0.a1 Prupe.8G101200_v2.0.a1 Prupe.8G101500_v2.0.a1 Prupe.8G105300_v2.0.a1 Prupe.8G105400_v2.0.a1 Prupe.8G105500_v2.0.a1 Prupe.8G105600_v2.0.a1
pyrus_communis	pycom04g19030 pycom04g19050 pycom04g19090 pycom04g19120 pycom05g05990 pycom05g06030 pycom10g06180 pycom10g06270 pycom10g06280 pycom10g06300 pycom10g06330 pycom10g06340 pycom12g02550 pycom17g26280
rosa_chinensis	RchiOBHm_Chr6g0263471 RchiOBHm_Chr6g0263621 RchiOBHm_Chr6g0263691
rosa_laevigata	RLG00000014328 RLG00000014330
rosa_multiflora	Rmu_co8086996.1_g000001 Rmu_co8446581.1_g000001 Rmu_sc0000565.1_g000052 Rmu_sc0010495.1_g000011
rosa_roxburghii	Rroxscaffold_176G00431110 Rroxscaffold_7G00204150
rosa_rugosa	Rorug06G0005300 Rorug06G0005700
rosa_samantha	Rh6AG124800 Rh6BG120900 Rh6CG119100 Rh6DG107300 Rh6DG107500
rosa_wichuraiana	Rw0G005290 Rw0G005320 Rw6G010850 Rw6G010870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	140, 1075, 1569
Acc65I	GGTACC	1 cut(s)	239
AccB1I	GGYRCC	1 cut(s)	239
AccB7I	CCANNNNNTGG	1 cut(s)	1248
AccBSI	CCGCTC	1 cut(s)	1332
AccI	GTMKAC	2 cut(s)	447, 1254
AccII	CGCG	2 cut(s)	1287, 1403
AciI	CCGC	5 cut(s)	270, 453, 968, 1332, 1403
AclI	AACGTT	1 cut(s)	516
AclWI	GGATC	4 cut(s)	68, 593, 962, 1458
AcoI	YGGCCR	4 cut(s)	43, 276, 864, 1311
AcuI	CTGAAG	1 cut(s)	744
AcvI	CACGTG	1 cut(s)	1248
AcyI	GRCGYC	5 cut(s)	137, 304, 690, 1072, 1566
AfaI	GTAC	8 cut(s)	241, 311, 321, 536, 702, 925, 944, 1203
AfiI	CCNNNNNNNGG	5 cut(s)	294, 862, 1165, 1248, 1320
AgsI	TTSAA	7 cut(s)	132, 221, 227, 247, 577, 1055, 1472
AjnI	CCWGG	1 cut(s)	950
AleI	CACNNNNGTG	1 cut(s)	1294
AluBI	AGCT	8 cut(s)	350, 440, 562, 739, 764, 994, 1260, 1547
AluI	AGCT	8 cut(s)	350, 440, 562, 739, 764, 994, 1260, 1547
Alw21I	GWGCWC	1 cut(s)	352
Alw26I	GTCTC	4 cut(s)	43, 186, 673, 681
AlwI	GGATC	4 cut(s)	68, 593, 962, 1458
AlwNI	CAGNNNCTG	1 cut(s)	932
Ama87I	CYCGRG	1 cut(s)	647
AoxI	GGCC	8 cut(s)	43, 276, 468, 864, 1176, 1311, 1368, 1386
ApeKI	GCWGC	6 cut(s)	736, 764, 816, 887, 1092, 1412
Asp700I	GAANNNNTTC	1 cut(s)	976
Asp718I	GGTACC	1 cut(s)	239
AspLEI	GCGC	4 cut(s)	605, 1287, 1289, 1417
AspS9I	GGNCC	4 cut(s)	1168, 1176, 1238, 1386
AsuHPI	GGTGA	3 cut(s)	645, 836, 1574
AvaI	CYCGRG	1 cut(s)	647
AvaII	GGWCC	2 cut(s)	1168, 1238
AxyI	CCTNAGG	1 cut(s)	501
BanI	GGYRCC	1 cut(s)	239
BanII	GRGCYC	1 cut(s)	352
BbrPI	CACGTG	1 cut(s)	1248
BbsI	GAAGAC	1 cut(s)	1420
Bbv12I	GWGCWC	1 cut(s)	352
BbvI	GCAGC	6 cut(s)	748, 751, 828, 899, 1104, 1399
BccI	CCATC	2 cut(s)	1250, 1379
BceAI	ACGGC	4 cut(s)	104, 851, 1163, 1298
BciT130I	CCWGG	1 cut(s)	952
BciVI	GTATCC	3 cut(s)	550, 908, 1455
BclI	TGATCA	2 cut(s)	1150, 1549
BcoDI	GTCTC	4 cut(s)	43, 186, 673, 681
BfaI	CTAG	2 cut(s)	623, 746
BfmI	CTRYAG	2 cut(s)	765, 1255
BfuI	GTATCC	3 cut(s)	550, 908, 1455
BglI	GCCNNNNNGGC	1 cut(s)	125
BglII	AGATCT	2 cut(s)	459, 1266
BisI	GCNGC	6 cut(s)	737, 765, 817, 888, 1093, 1413
BlsI	GCNGC	6 cut(s)	738, 766, 818, 889, 1094, 1414
BmcAI	AGTACT	2 cut(s)	321, 702
Bme1390I	CCNGG	1 cut(s)	952
Bme18I	GGWCC	2 cut(s)	1168, 1238
BmeT110I	CYCGRG	1 cut(s)	647
BmgT120I	GGNCC	4 cut(s)	1168, 1176, 1238, 1386
BmiI	GGNNCC	6 cut(s)	241, 268, 1170, 1239, 1240, 1387
BmrFI	CCNGG	1 cut(s)	952
BmrI	ACTGGG	2 cut(s)	158, 539
BmsI	GCATC	2 cut(s)	606, 1200
BmuI	ACTGGG	2 cut(s)	158, 539
BpiI	GAAGAC	1 cut(s)	1420
BplI	GAGNNNNNCTC	4 cut(s)	168, 200, 339, 371
BpmI	CTGGAG	1 cut(s)	601
Bpu10I	CCTNAGC	1 cut(s)	553
BsaAI	YACGTR	2 cut(s)	538, 1248
BsaHI	GRCGYC	5 cut(s)	137, 304, 690, 1072, 1566
BsaJI	CCNNGG	8 cut(s)	273, 628, 820, 892, 950, 1005, 1172, 1197
BsaXI	ACNNNNNCTCC	6 cut(s)	23, 53, 402, 432, 1472, 1502
Bsc4I	CCNNNNNNNGG	5 cut(s)	294, 862, 1165, 1248, 1320
Bse118I	RCCGGY	3 cut(s)	278, 306, 862
Bse1I	ACTGG	4 cut(s)	153, 299, 534, 584
Bse21I	CCTNAGG	1 cut(s)	501
Bse3DI	GCAATG	2 cut(s)	402, 1029
BseBI	CCWGG	1 cut(s)	952
BseDI	CCNNGG	8 cut(s)	273, 628, 820, 892, 950, 1005, 1172, 1197
BseGI	GGATG	1 cut(s)	796
BseLI	CCNNNNNNNGG	5 cut(s)	294, 862, 1165, 1248, 1320
BseMI	GCAATG	2 cut(s)	402, 1029
BseMII	CTCAG	4 cut(s)	492, 627, 754, 1254
BseNI	ACTGG	4 cut(s)	153, 299, 534, 584
BsePI	GCGCGC	1 cut(s)	1285
BseRI	GAGGAG	1 cut(s)	344
BseX3I	CGGCCG	1 cut(s)	864
BseXI	GCAGC	6 cut(s)	748, 751, 828, 899, 1104, 1399
BsgI	GTGCAG	1 cut(s)	1318
Bsh1236I	CGCG	2 cut(s)	1287, 1403
Bsh1285I	CGRYCG	2 cut(s)	867, 1146
BshFI	GGCC	8 cut(s)	45, 278, 470, 866, 1178, 1313, 1370, 1388
BshNI	GGYRCC	1 cut(s)	239
BsiEI	CGRYCG	2 cut(s)	867, 1146
BsiHKAI	GWGCWC	1 cut(s)	352
BsiHKCI	CYCGRG	1 cut(s)	647
BsiSI	CCGG	7 cut(s)	42, 279, 307, 327, 408, 467, 863
BslFI	GGGAC	2 cut(s)	1154, 1251
BslI	CCNNNNNNNGG	5 cut(s)	294, 862, 1165, 1248, 1320
BsmAI	GTCTC	4 cut(s)	43, 186, 673, 681
BsmBI	CGTCTC	3 cut(s)	43, 186, 681
BsmFI	GGGAC	2 cut(s)	1154, 1251
BsnI	GGCC	8 cut(s)	45, 278, 470, 866, 1178, 1313, 1370, 1388
BsoBI	CYCGRG	1 cut(s)	647
Bsp1286I	GDGCHC	1 cut(s)	352
Bsp1407I	TGTACA	1 cut(s)	942
Bsp143I	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
Bsp19I	CCATGG	2 cut(s)	1172, 1197
BspACI	CCGC	5 cut(s)	270, 453, 968, 1332, 1403
BspANI	GGCC	8 cut(s)	45, 278, 470, 866, 1178, 1313, 1370, 1388
BspCNI	CTCAG	4 cut(s)	493, 626, 753, 1255
BspFNI	CGCG	2 cut(s)	1287, 1403
BspLI	GGNNCC	6 cut(s)	241, 268, 1170, 1239, 1240, 1387
BspMAI	CTGCAG	1 cut(s)	769
BspPI	GGATC	4 cut(s)	68, 593, 962, 1458
BspT107I	GGYRCC	1 cut(s)	239
BsrBI	CCGCTC	1 cut(s)	1332
BsrDI	GCAATG	2 cut(s)	402, 1029
BsrFI	RCCGGY	3 cut(s)	278, 306, 862
BsrGI	TGTACA	1 cut(s)	942
BsrI	ACTGG	4 cut(s)	153, 299, 534, 584
BssAI	RCCGGY	3 cut(s)	278, 306, 862
BssECI	CCNNGG	8 cut(s)	273, 628, 820, 892, 950, 1005, 1172, 1197
BssHII	GCGCGC	1 cut(s)	1285
BssMI	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
BssNI	GRCGYC	5 cut(s)	137, 304, 690, 1072, 1566
BssT1I	CCWWGG	5 cut(s)	273, 820, 892, 1172, 1197
Bst2UI	CCWGG	1 cut(s)	952
Bst4CI	ACNGT	3 cut(s)	239, 1006, 1144
Bst6I	CTCTTC	2 cut(s)	191, 720
BstACI	GRCGYC	5 cut(s)	137, 304, 690, 1072, 1566
BstAUI	TGTACA	1 cut(s)	942
BstBAI	YACGTR	2 cut(s)	538, 1248
BstC8I	GCNNGC	4 cut(s)	662, 864, 1287, 1545
BstDEI	CTNAG	7 cut(s)	501, 553, 613, 740, 1263, 1418, 1539
BstDSI	CCRYGG	3 cut(s)	1005, 1172, 1197
BstF5I	GGATG	1 cut(s)	796
BstFNI	CGCG	2 cut(s)	1287, 1403
BstHHI	GCGC	4 cut(s)	605, 1287, 1289, 1417
BstKTI	GATC	9 cut(s)	76, 462, 588, 957, 1063, 1153, 1269, 1466, 1552
BstMAI	GTCTC	4 cut(s)	43, 186, 673, 681
BstMBI	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
BstMCI	CGRYCG	2 cut(s)	867, 1146
BstMWI	GCNNNNNNNGC	4 cut(s)	125, 670, 1007, 1409
BstNI	CCWGG	1 cut(s)	952
BstSCI	CCNGG	1 cut(s)	950
BstSFI	CTRYAG	2 cut(s)	765, 1255
BstSNI	TACGTA	1 cut(s)	538
BstUI	CGCG	2 cut(s)	1287, 1403
BstV1I	GCAGC	6 cut(s)	748, 751, 828, 899, 1104, 1399
BstV2I	GAAGAC	1 cut(s)	1420
BstX2I	RGATCY	3 cut(s)	459, 954, 1266
BstXI	CCANNNNNNTGG	1 cut(s)	1234
BstYI	RGATCY	3 cut(s)	459, 954, 1266
BstZI	CGGCCG	1 cut(s)	864
Bsu36I	CCTNAGG	1 cut(s)	501
BsuI	GTATCC	3 cut(s)	550, 908, 1455
BsuRI	GGCC	8 cut(s)	45, 278, 470, 866, 1178, 1313, 1370, 1388
BtgI	CCRYGG	3 cut(s)	1005, 1172, 1197
BtgZI	GCGATG	2 cut(s)	228, 1177
BtsCI	GGATG	1 cut(s)	796
Cac8I	GCNNGC	4 cut(s)	662, 864, 1287, 1545
CaiI	CAGNNNCTG	1 cut(s)	932
CfoI	GCGC	4 cut(s)	605, 1287, 1289, 1417
Cfr10I	RCCGGY	3 cut(s)	278, 306, 862
Cfr13I	GGNCC	4 cut(s)	1168, 1176, 1238, 1386
CseI	GACGC	3 cut(s)	168, 312, 698
Csp6I	GTAC	8 cut(s)	240, 310, 320, 535, 701, 924, 943, 1202
CspCI	CAANNNNNGTGG	2 cut(s)	1023, 1058
CviQI	GTAC	8 cut(s)	240, 310, 320, 535, 701, 924, 943, 1202
DdeI	CTNAG	7 cut(s)	501, 553, 613, 740, 1263, 1418, 1539
DpnI	GATC	9 cut(s)	75, 461, 587, 956, 1062, 1152, 1268, 1465, 1551
DpnII	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
EaeI	YGGCCR	4 cut(s)	43, 276, 864, 1311
EagI	CGGCCG	1 cut(s)	864
Eam1104I	CTCTTC	2 cut(s)	191, 720
EarI	CTCTTC	2 cut(s)	191, 720
EciI	GGCGGA	1 cut(s)	259
Ecl136II	GAGCTC	1 cut(s)	350
EclXI	CGGCCG	1 cut(s)	864
Eco105I	TACGTA	1 cut(s)	538
Eco130I	CCWWGG	5 cut(s)	273, 820, 892, 1172, 1197
Eco24I	GRGCYC	1 cut(s)	352
Eco47I	GGWCC	2 cut(s)	1168, 1238
Eco52I	CGGCCG	1 cut(s)	864
Eco53kI	GAGCTC	1 cut(s)	350
Eco57I	CTGAAG	1 cut(s)	744
Eco72I	CACGTG	1 cut(s)	1248
Eco81I	CCTNAGG	1 cut(s)	501
Eco88I	CYCGRG	1 cut(s)	647
EcoICRI	GAGCTC	1 cut(s)	350
EcoO109I	RGGNCCY	3 cut(s)	1168, 1238, 1386
EcoRII	CCWGG	1 cut(s)	950
EcoT14I	CCWWGG	5 cut(s)	273, 820, 892, 1172, 1197
EcoT38I	GRGCYC	1 cut(s)	352
ErhI	CCWWGG	5 cut(s)	273, 820, 892, 1172, 1197
Esp3I	CGTCTC	3 cut(s)	43, 186, 681
FaqI	GGGAC	2 cut(s)	1154, 1251
FauI	CCCGC	3 cut(s)	961, 1325, 1396
FbaI	TGATCA	2 cut(s)	1150, 1549
FblI	GTMKAC	2 cut(s)	447, 1254
Fnu4HI	GCNGC	6 cut(s)	737, 765, 817, 888, 1093, 1413
FokI	GGATG	1 cut(s)	803
FriOI	GRGCYC	1 cut(s)	352
Fsp4HI	GCNGC	6 cut(s)	737, 765, 817, 888, 1093, 1413
FspBI	CTAG	2 cut(s)	623, 746
GlaI	GCGC	4 cut(s)	604, 1286, 1288, 1416
GluI	GCNGC	6 cut(s)	737, 765, 817, 888, 1093, 1413
GsuI	CTGGAG	1 cut(s)	601
HaeIII	GGCC	8 cut(s)	45, 278, 470, 866, 1178, 1313, 1370, 1388
HapII	CCGG	7 cut(s)	42, 279, 307, 327, 408, 467, 863
HgaI	GACGC	3 cut(s)	168, 312, 698
HhaI	GCGC	4 cut(s)	605, 1287, 1289, 1417
Hin1I	GRCGYC	5 cut(s)	137, 304, 690, 1072, 1566
Hin6I	GCGC	4 cut(s)	603, 1285, 1287, 1415
HinP1I	GCGC	4 cut(s)	603, 1285, 1287, 1415
HincII	GTYRAC	3 cut(s)	514, 1326, 1570
HindII	GTYRAC	3 cut(s)	514, 1326, 1570
HinfI	GANTC	8 cut(s)	399, 650, 681, 731, 771, 782, 929, 972
HpaI	GTTAAC	1 cut(s)	514
HpaII	CCGG	7 cut(s)	42, 279, 307, 327, 408, 467, 863
HphI	GGTGA	3 cut(s)	645, 836, 1574
Hpy166II	GTNNAC	7 cut(s)	312, 448, 514, 1255, 1318, 1326, 1570
Hpy188I	TCNGA	6 cut(s)	292, 686, 724, 743, 1070, 1264
Hpy188III	TCNNGA	4 cut(s)	961, 1024, 1375, 1448
Hpy8I	GTNNAC	7 cut(s)	312, 448, 514, 1255, 1318, 1326, 1570
Hpy99I	CGWCG	3 cut(s)	38, 1077, 1184
HpyAV	CCTTC	4 cut(s)	391, 821, 832, 1399
HpyCH4III	ACNGT	3 cut(s)	239, 1006, 1144
HpyCH4IV	ACGT	8 cut(s)	33, 137, 516, 537, 1072, 1247, 1439, 1566
HpyCH4V	TGCA	7 cut(s)	673, 767, 1001, 1034, 1191, 1279, 1299
HpyF10VI	GCNNNNNNNGC	4 cut(s)	125, 670, 1007, 1409
HpyF3I	CTNAG	7 cut(s)	501, 553, 613, 740, 1263, 1418, 1539
HpySE526I	ACGT	8 cut(s)	33, 137, 516, 537, 1072, 1247, 1439, 1566
Hsp92I	GRCGYC	5 cut(s)	137, 304, 690, 1072, 1566
HspAI	GCGC	4 cut(s)	603, 1285, 1287, 1415
KflI	GGGWCCC	1 cut(s)	1238
KpnI	GGTACC	1 cut(s)	243
KroI	GCCGGC	1 cut(s)	862
KroNI	GCCGGC	1 cut(s)	864
Ksp22I	TGATCA	2 cut(s)	1150, 1549
KspAI	GTTAAC	1 cut(s)	514
Kzo9I	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
LmnI	GCTCC	5 cut(s)	176, 272, 347, 445, 567
Lsp1109I	GCAGC	6 cut(s)	748, 751, 828, 899, 1104, 1399
LweI	GCATC	2 cut(s)	606, 1200
MaeI	CTAG	2 cut(s)	623, 746
MaeII	ACGT	8 cut(s)	33, 137, 516, 537, 1072, 1247, 1439, 1566
MaeIII	GTNAC	2 cut(s)	651, 1562
MalI	GATC	9 cut(s)	75, 461, 587, 956, 1062, 1152, 1268, 1465, 1551
MbiI	CCGCTC	1 cut(s)	1332
MboI	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
MboII	GAAGA	8 cut(s)	178, 328, 711, 737, 898, 949, 1420, 1484
MflI	RGATCY	3 cut(s)	459, 954, 1266
MhlI	GDGCHC	1 cut(s)	352
MluCI	AATT	3 cut(s)	606, 842, 1407
MlyI	GAGTC	4 cut(s)	659, 675, 776, 923
MmeI	TCCRAC	4 cut(s)	394, 567, 934, 994
MroNI	GCCGGC	1 cut(s)	862
MroXI	GAANNNNTTC	1 cut(s)	976
MseI	TTAA	6 cut(s)	234, 513, 756, 881, 1088, 1530
MslI	CAYNNNNRTG	3 cut(s)	1196, 1294, 1343
MspI	CCGG	7 cut(s)	42, 279, 307, 327, 408, 467, 863
MspR9I	CCNGG	1 cut(s)	952
MvaI	CCWGG	1 cut(s)	952
MvnI	CGCG	2 cut(s)	1287, 1403
MwoI	GCNNNNNNNGC	4 cut(s)	125, 670, 1007, 1409
NaeI	GCCGGC	1 cut(s)	864
NcoI	CCATGG	2 cut(s)	1172, 1197
NdeII	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
NgoMIV	GCCGGC	1 cut(s)	862
NlaIV	GGNNCC	6 cut(s)	241, 268, 1170, 1239, 1240, 1387
NmeAIII	GCCGAG	3 cut(s)	481, 573, 609
NmuCI	GTSAC	2 cut(s)	651, 1562
OliI	CACNNNNGTG	1 cut(s)	1294
PauI	GCGCGC	1 cut(s)	1285
PcsI	WCGNNNNNNNCGW	1 cut(s)	1069
PdiI	GCCGGC	1 cut(s)	864
PdmI	GAANNNNTTC	1 cut(s)	976
PfeI	GAWTC	4 cut(s)	399, 731, 771, 972
PflMI	CCANNNNNTGG	1 cut(s)	1248
PkrI	GCNGC	6 cut(s)	738, 766, 818, 889, 1094, 1414
PleI	GAGTC	4 cut(s)	658, 675, 776, 923
PmaCI	CACGTG	1 cut(s)	1248
PmlI	CACGTG	1 cut(s)	1248
PpsI	GAGTC	4 cut(s)	658, 675, 776, 923
Ppu21I	YACGTR	2 cut(s)	538, 1248
PpuMI	RGGWCCY	2 cut(s)	1168, 1238
Psp124BI	GAGCTC	1 cut(s)	352
Psp1406I	AACGTT	1 cut(s)	516
Psp5II	RGGWCCY	2 cut(s)	1168, 1238
Psp6I	CCWGG	1 cut(s)	950
PspCI	CACGTG	1 cut(s)	1248
PspGI	CCWGG	1 cut(s)	950
PspN4I	GGNNCC	6 cut(s)	241, 268, 1170, 1239, 1240, 1387
PspPI	GGNCC	4 cut(s)	1168, 1176, 1238, 1386
PspPPI	RGGWCCY	2 cut(s)	1168, 1238
PstI	CTGCAG	1 cut(s)	769
PstNI	CAGNNNCTG	1 cut(s)	932
PsuI	RGATCY	3 cut(s)	459, 954, 1266
PteI	GCGCGC	1 cut(s)	1285
RsaI	GTAC	8 cut(s)	241, 311, 321, 536, 702, 925, 944, 1203
RsaNI	GTAC	8 cut(s)	240, 310, 320, 535, 701, 924, 943, 1202
RseI	CAYNNNNRTG	3 cut(s)	1196, 1294, 1343
SacI	GAGCTC	1 cut(s)	352
SaqAI	TTAA	6 cut(s)	234, 513, 756, 881, 1088, 1530
SatI	GCNGC	6 cut(s)	737, 765, 817, 888, 1093, 1413
Sau3AI	GATC	9 cut(s)	73, 459, 585, 954, 1060, 1150, 1266, 1463, 1549
Sau96I	GGNCC	4 cut(s)	1168, 1176, 1238, 1386
ScaI	AGTACT	2 cut(s)	321, 702
SchI	GAGTC	4 cut(s)	659, 675, 776, 923
ScrFI	CCNGG	1 cut(s)	952
SduI	GDGCHC	1 cut(s)	352
SfaNI	GCATC	2 cut(s)	606, 1200
SfcI	CTRYAG	2 cut(s)	765, 1255
SinI	GGWCC	2 cut(s)	1168, 1238
SmiMI	CAYNNNNRTG	3 cut(s)	1196, 1294, 1343
SnaBI	TACGTA	1 cut(s)	538
Sse9I	AATT	3 cut(s)	606, 842, 1407
SsiI	CCGC	5 cut(s)	270, 453, 968, 1332, 1403
SspMI	CTAG	2 cut(s)	623, 746
SstI	GAGCTC	1 cut(s)	352
StyD4I	CCNGG	1 cut(s)	950
StyI	CCWWGG	5 cut(s)	273, 820, 892, 1172, 1197
TaaI	ACNGT	3 cut(s)	239, 1006, 1144
TaiI	ACGT	8 cut(s)	36, 140, 519, 540, 1075, 1250, 1442, 1569
TaqI	TCGA	2 cut(s)	960, 1462
TasI	AATT	3 cut(s)	606, 842, 1407
TatI	WGTACW	3 cut(s)	319, 700, 942
TfiI	GAWTC	4 cut(s)	399, 731, 771, 972
Tru1I	TTAA	6 cut(s)	234, 513, 756, 881, 1088, 1530
Tru9I	TTAA	6 cut(s)	234, 513, 756, 881, 1088, 1530
TseFI	GTSAC	2 cut(s)	651, 1562
TseI	GCWGC	6 cut(s)	736, 764, 816, 887, 1092, 1412
Tsp45I	GTSAC	2 cut(s)	651, 1562
TspDTI	ATGAA	4 cut(s)	178, 391, 1198, 1347
TspGWI	ACGGA	1 cut(s)	1263
Van91I	CCANNNNNTGG	1 cut(s)	1248
VpaK11BI	GGWCC	2 cut(s)	1168, 1238
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1036
XmiI	GTMKAC	2 cut(s)	447, 1254
XmnI	GAANNNNTTC	1 cut(s)	976
XspI	CTAG	2 cut(s)	623, 746
ZraI	GACGTC	3 cut(s)	138, 1073, 1567
ZrmI	AGTACT	2 cut(s)	321, 702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.