pycom04g20790

Translational activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0004626
Physical Location & Seq
Reverse (-)
22219760 .. 22222463
2704 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
ATGGAAAAACAAGCGAGGCTGCAATCACATGTTGTAGGAGTAAGTTTGCCGGTCTCGAAGAATGCTATGTGTAGAGTTGCCACAGTGCAGGCATTGTTACTTAATCTTGTTATGCAGAGGTCTTTCCATGACCGAAGGGCTTACAAGAAGACATTTTGTCCCTTATTTTCTCAGTCACCAGACATCTACAAAATGTATATAGAGGAACTCAAAGATGCCCGTATCCCATACAAAGATAGTTCTGAATTAATATGGTTGTTATTAGAGTGCTCAAGTACCTCAAGCAAATCATCTTCTTTGTATGAACAATGCAAGCCAACATTTTTGGATATATATCTCAAGGCAATCTTAAATGCAAGAGAAAAGCCAGCAAGGGGCCTAAGTGAAGCTTTTCATCCACTTTTTAAACATATGTTGCACGAAGATTTTCAAAATATTGTGCTTCCATCTGCTGTTAAAATGCTGAAACGCAACCCTGAGATTGTATTGGAGTCGGTTGGGATTCTGTTGAAATCAGTCAACCTTGATTTAAGCAAGTATGCCGTTGAAATTCTTTCAGTGTATTGCCTCAGGCTCAGCATGCGGATGAAGGCAGAAGGGTTGTGGCACTGGCAGTTATACGATGCTTGA

Protein Analysis

210

Amino Acids

24.11

Weight (kDa)

9.13

Isoelectric Point (pI)

59.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GNC1_N PF24993 105 - 187 1.3e-28 Stalled ribosome sensor GCN1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 583
AcsI RAATTY 1 cut(s) 549
AfaI GTAC 1 cut(s) 277
AfiI CCNNNNNNNGG 1 cut(s) 374
AflIII ACRYGT 1 cut(s) 28
AgsI TTSAA 3 cut(s) 431, 511, 548
AhdI GACNNNNNGTC 1 cut(s) 156
AluBI AGCT 1 cut(s) 389
AluI AGCT 1 cut(s) 389
Alw21I GWGCWC 1 cut(s) 272
Alw26I GTCTC 1 cut(s) 58
AoxI GGCC 1 cut(s) 376
ApeKI GCWGC 1 cut(s) 19
ApoI RAATTY 1 cut(s) 549
AseI ATTAAT 1 cut(s) 248
Asp700I GAANNNNTTC 2 cut(s) 390, 426
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 168
AxyI CCTNAGG 1 cut(s) 569
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 272
BbvI GCAGC 1 cut(s) 6
BccI CCATC 1 cut(s) 454
BceAI ACGGC 1 cut(s) 527
BciVI GTATCC 1 cut(s) 233
BcoDI GTCTC 1 cut(s) 58
BfuI GTATCC 1 cut(s) 233
BisI GCNGC 1 cut(s) 20
BlpI GCTNAGC 1 cut(s) 575
BlsI GCNGC 1 cut(s) 21
BmeRI GACNNNNNGTC 1 cut(s) 156
BmgT120I GGNCC 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 377
BmsI GCATC 2 cut(s) 205, 613
BpiI GAAGAC 1 cut(s) 155
Bpu1102I GCTNAGC 1 cut(s) 575
BpuEI CTTGAG 3 cut(s) 256, 265, 323
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 1 cut(s) 58
Bsc4I CCNNNNNNNGG 1 cut(s) 374
Bse118I RCCGGY 1 cut(s) 49
Bse1I ACTGG 1 cut(s) 614
Bse21I CCTNAGG 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 333
BseGI GGATG 2 cut(s) 394, 591
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 1 cut(s) 374
BseMII CTCAG 4 cut(s) 185, 468, 583, 589
BseNI ACTGG 1 cut(s) 614
BseXI GCAGC 1 cut(s) 6
BsgI GTGCAG 1 cut(s) 107
BshFI GGCC 1 cut(s) 378
BsiHKAI GWGCWC 1 cut(s) 272
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 1 cut(s) 144
BslI CCNNNNNNNGG 1 cut(s) 374
BsmAI GTCTC 1 cut(s) 58
BsmFI GGGAC 1 cut(s) 144
BsmI GAATGC 1 cut(s) 67
BsnI GGCC 1 cut(s) 378
Bso31I GGTCTC 1 cut(s) 58
Bsp1286I GDGCHC 1 cut(s) 272
Bsp1720I GCTNAGC 1 cut(s) 575
BspACI CCGC 1 cut(s) 583
BspANI GGCC 1 cut(s) 378
BspCNI CTCAG 4 cut(s) 184, 469, 582, 588
BspLI GGNNCC 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 58
BsrFI RCCGGY 1 cut(s) 49
BsrI ACTGG 1 cut(s) 614
BssAI RCCGGY 1 cut(s) 49
Bst4CI ACNGT 1 cut(s) 85
BstC8I GCNNGC 4 cut(s) 90, 314, 369, 581
BstDEI CTNAG 5 cut(s) 171, 380, 477, 569, 575
BstF5I GGATG 2 cut(s) 394, 591
BstMAI GTCTC 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 580
BstNSI RCATGY 2 cut(s) 32, 583
BstV1I GCAGC 1 cut(s) 6
BstV2I GAAGAC 1 cut(s) 155
Bsu36I CCTNAGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 233
BsuRI GGCC 1 cut(s) 378
BtsCI GGATG 2 cut(s) 394, 591
BtsIMutI CAGTG 3 cut(s) 90, 564, 607
Cac8I GCNNGC 4 cut(s) 90, 314, 369, 581
Cfr10I RCCGGY 1 cut(s) 49
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 1 cut(s) 276
CviAII CATG 3 cut(s) 29, 128, 580
CviJI RGCY 7 cut(s) 19, 140, 316, 367, 378, 389, 574
CviKI_1 RGCY 7 cut(s) 19, 140, 316, 367, 378, 389, 574
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 5 cut(s) 171, 380, 477, 569, 575
DraI TTTAAA 1 cut(s) 406
DriI GACNNNNNGTC 1 cut(s) 156
Eam1105I GACNNNNNGTC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 58
Eco81I CCTNAGG 1 cut(s) 569
EcoO109I RGGNCCY 1 cut(s) 376
FaeI CATG 3 cut(s) 32, 131, 583
FalI AAGNNNNNCTT 4 cut(s) 332, 364, 373, 405
FaqI GGGAC 1 cut(s) 144
FatI CATG 3 cut(s) 28, 127, 579
FauNDI CATATG 1 cut(s) 411
Fnu4HI GCNGC 1 cut(s) 20
FokI GGATG 2 cut(s) 381, 598
Fsp4HI GCNGC 1 cut(s) 20
GluI GCNGC 1 cut(s) 20
HaeIII GGCC 1 cut(s) 378
HapII CCGG 1 cut(s) 50
Hin1II CATG 3 cut(s) 32, 131, 583
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HindIII AAGCTT 1 cut(s) 387
HinfI GANTC 2 cut(s) 491, 502
HpaII CCGG 1 cut(s) 50
HphI GGTGA 1 cut(s) 168
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 1 cut(s) 520
HpyAV CCTTC 3 cut(s) 129, 583, 590
HpyCH4III ACNGT 1 cut(s) 85
HpyCH4V TGCA 6 cut(s) 22, 88, 115, 312, 356, 418
HpyF10VI GCNNNNNNNGC 1 cut(s) 580
HpyF3I CTNAG 5 cut(s) 171, 380, 477, 569, 575
Hsp92II CATG 3 cut(s) 32, 131, 583
LpnPI CCDG 7 cut(s) 63, 74, 192, 381, 489, 556, 595
Lsp1109I GCAGC 1 cut(s) 6
LweI GCATC 2 cut(s) 205, 613
MaeIII GTNAC 2 cut(s) 96, 174
MboII GAAGA 4 cut(s) 70, 160, 285, 434
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 2 cut(s) 245, 549
MlyI GAGTC 1 cut(s) 500
MnlI CCTC 5 cut(s) 9, 111, 196, 289, 578
MroXI GAANNNNTTC 2 cut(s) 390, 426
MseI TTAA 6 cut(s) 102, 248, 350, 405, 456, 530
MslI CAYNNNNRTG 1 cut(s) 584
MspI CCGG 1 cut(s) 50
Mva1269I GAATGC 1 cut(s) 67
MwoI GCNNNNNNNGC 1 cut(s) 580
NdeI CATATG 1 cut(s) 411
NlaIII CATG 3 cut(s) 32, 131, 583
NlaIV GGNNCC 1 cut(s) 377
NmuCI GTSAC 1 cut(s) 174
NspI RCATGY 2 cut(s) 32, 583
PaeI GCATGC 1 cut(s) 583
PciI ACATGT 1 cut(s) 28
PctI GAATGC 1 cut(s) 67
PdmI GAANNNNTTC 2 cut(s) 390, 426
PfeI GAWTC 1 cut(s) 502
PkrI GCNGC 1 cut(s) 21
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PscI ACATGT 1 cut(s) 28
PshBI ATTAAT 1 cut(s) 248
PspN4I GGNNCC 1 cut(s) 377
PspPI GGNCC 1 cut(s) 376
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 584
SaqAI TTAA 6 cut(s) 102, 248, 350, 405, 456, 530
SatI GCNGC 1 cut(s) 20
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 1 cut(s) 500
SduI GDGCHC 1 cut(s) 272
SetI ASST 4 cut(s) 122, 281, 391, 525
SfaNI GCATC 2 cut(s) 205, 613
SmiMI CAYNNNNRTG 1 cut(s) 584
SmlI CTYRAG 3 cut(s) 271, 280, 338
SmoI CTYRAG 3 cut(s) 271, 280, 338
SphI GCATGC 1 cut(s) 583
Sse9I AATT 2 cut(s) 245, 549
SsiI CCGC 1 cut(s) 583
SspI AATATT 1 cut(s) 436
TaaI ACNGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 56
TaqII GACCGA 1 cut(s) 147
TasI AATT 2 cut(s) 245, 549
TfiI GAWTC 1 cut(s) 502
Tru1I TTAA 6 cut(s) 102, 248, 350, 405, 456, 530
Tru9I TTAA 6 cut(s) 102, 248, 350, 405, 456, 530
TscAI CASTG 3 cut(s) 90, 564, 614
TseFI GTSAC 1 cut(s) 174
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 1 cut(s) 174
TspDTI ATGAA 3 cut(s) 318, 383, 602
TspRI CASTG 3 cut(s) 90, 564, 614
VspI ATTAAT 1 cut(s) 248
XapI RAATTY 1 cut(s) 549
XceI RCATGY 2 cut(s) 32, 583
XmnI GAANNNNTTC 2 cut(s) 390, 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.