pycom05g09280

Gibberellin regulated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002945
Physical Location & Seq
Reverse (-)
12403578 .. 12404482
905 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 444 bp
ATGATAATCGAACTTAAACCGTCAACCAAAGAAATACCACTACGTAATACTAGTTGTTGCCGACTCACAAGTTCTCTCTCACTCGCTATTAAATACCAGCTTGAGCTCTCACAATCCTCCACACTGCCTCAAGTACACTTCTCAACTACGACTTGTCCTTTGAGTTCACCAGGCAAGATGAAGCTCTACTTTCCACTCCTCTGTTTCCTTCTTCTTACCTCTTCTTTCATGGATCCAGCCATGGCCCTCGCTACTTCACCATATTGCAGCGACAAGTGCTCGACGAGGTGTGCAGTAGCAGGAGTTCGGGATCGATGCATAAAGTATTGTGGGATTTGCTGTGAAGCGTGCAAGTGTGTGCCTTCTGGGACTTATGGGAACAAGCACGAGTGCCCTTGCTACAGGGACAAGAAGAACGACAAGGGCAAGCCCAAGTGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GASA

Protein Analysis

148

Amino Acids

16.2

Weight (kDa)

8.94

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GASA PF02704 89 - 147 6.9e-21 Gibberellin regulated protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 227, 240, 318
AfaI GTAC 1 cut(s) 135
AhlI ACTAGT 1 cut(s) 50
AjnI CCWGG 1 cut(s) 169
AluBI AGCT 3 cut(s) 100, 106, 184
AluI AGCT 3 cut(s) 100, 106, 184
Alw21I GWGCWC 2 cut(s) 108, 281
AlwI GGATC 3 cut(s) 227, 240, 318
AoxI GGCC 1 cut(s) 243
ApeKI GCWGC 1 cut(s) 267
ArsI GACNNNNNNTTYG 2 cut(s) 142, 174
AspS9I GGNCC 1 cut(s) 244
AsuHPI GGTGA 2 cut(s) 159, 249
BaeGI GKGCMC 2 cut(s) 395, 440
BamHI GGATCC 1 cut(s) 232
BanII GRGCYC 1 cut(s) 108
BauI CACGAG 1 cut(s) 386
Bbv12I GWGCWC 2 cut(s) 108, 281
BbvI GCAGC 1 cut(s) 279
BciT130I CCWGG 1 cut(s) 171
BcuI ACTAGT 1 cut(s) 50
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 1 cut(s) 400
BisI GCNGC 1 cut(s) 268
BlsI GCNGC 1 cut(s) 269
Bme1390I CCNGG 1 cut(s) 171
BmgT120I GGNCC 1 cut(s) 244
BmiI GGNNCC 1 cut(s) 234
BmrFI CCNGG 1 cut(s) 171
BmsI GCATC 1 cut(s) 305
BpuEI CTTGAG 2 cut(s) 114, 122
Bsa29I ATCGAT 1 cut(s) 313
BsaAI YACGTR 1 cut(s) 44
BsaJI CCNNGG 1 cut(s) 240
BseBI CCWGG 1 cut(s) 171
BseCI ATCGAT 1 cut(s) 313
BseDI CCNNGG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 188
BseSI GKGCMC 2 cut(s) 395, 440
BseXI GCAGC 1 cut(s) 279
BsgI GTGCAG 1 cut(s) 312
BshFI GGCC 1 cut(s) 245
BshVI ATCGAT 1 cut(s) 313
BsiHKAI GWGCWC 2 cut(s) 108, 281
BslFI GGGAC 2 cut(s) 382, 419
BsmFI GGGAC 2 cut(s) 382, 419
BsnI GGCC 1 cut(s) 245
Bsp1286I GDGCHC 4 cut(s) 108, 281, 395, 440
Bsp143I GATC 2 cut(s) 232, 310
Bsp19I CCATGG 1 cut(s) 240
BspANI GGCC 1 cut(s) 245
BspDI ATCGAT 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 234
BspPI GGATC 3 cut(s) 227, 240, 318
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 2 cut(s) 232, 310
BssSI CACGAG 1 cut(s) 386
BssT1I CCWWGG 1 cut(s) 240
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 171
Bst4CI ACNGT 1 cut(s) 21
Bst6I CTCTTC 1 cut(s) 226
BstBAI YACGTR 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 349, 428
BstDSI CCRYGG 1 cut(s) 240
BstKTI GATC 2 cut(s) 235, 313
BstMBI GATC 2 cut(s) 232, 310
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNI CCWGG 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 169
BstSFI CTRYAG 1 cut(s) 400
BstSLI GKGCMC 2 cut(s) 395, 440
BstSNI TACGTA 1 cut(s) 44
BstV1I GCAGC 1 cut(s) 279
BstX2I RGATCY 1 cut(s) 232
BstYI RGATCY 1 cut(s) 232
Bsu15I ATCGAT 1 cut(s) 313
BsuRI GGCC 1 cut(s) 245
BsuTUI ATCGAT 1 cut(s) 313
BtgI CCRYGG 1 cut(s) 240
BtsI GCAGTG 1 cut(s) 122
BtsIMutI CAGTG 1 cut(s) 122
Cac8I GCNNGC 2 cut(s) 349, 428
Cfr13I GGNCC 1 cut(s) 244
ClaI ATCGAT 1 cut(s) 313
Csp6I GTAC 1 cut(s) 134
CviAII CATG 2 cut(s) 229, 241
CviJI RGCY 6 cut(s) 100, 106, 184, 239, 245, 430
CviKI_1 RGCY 6 cut(s) 100, 106, 184, 239, 245, 430
CviQI GTAC 1 cut(s) 134
DpnI GATC 2 cut(s) 234, 312
DpnII GATC 2 cut(s) 232, 310
Eam1104I CTCTTC 1 cut(s) 226
EarI CTCTTC 1 cut(s) 226
Ecl136II GAGCTC 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 240
Eco24I GRGCYC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 106
EcoICRI GAGCTC 1 cut(s) 106
EcoRII CCWGG 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 240
EcoT22I ATGCAT 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 108
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 2 cut(s) 232, 244
FaiI YATR 5 cut(s) 230, 242, 262, 320, 375
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 2 cut(s) 382, 419
FatI CATG 2 cut(s) 228, 240
Fnu4HI GCNGC 1 cut(s) 268
FriOI GRGCYC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 268
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 268
HaeIII GGCC 1 cut(s) 245
Hin1II CATG 2 cut(s) 232, 244
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 1 cut(s) 63
HphI GGTGA 2 cut(s) 159, 249
Hpy166II GTNNAC 3 cut(s) 24, 136, 167
Hpy188III TCNNGA 1 cut(s) 308
Hpy8I GTNNAC 3 cut(s) 24, 136, 167
Hpy99I CGWCG 1 cut(s) 286
HpyAV CCTTC 2 cut(s) 218, 372
HpyCH4III ACNGT 1 cut(s) 21
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 4 cut(s) 267, 293, 318, 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 2 cut(s) 232, 244
Kzo9I GATC 2 cut(s) 232, 310
LpnPI CCDG 7 cut(s) 110, 156, 183, 249, 285, 351, 388
Lsp1109I GCAGC 1 cut(s) 279
LweI GCATC 1 cut(s) 305
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 43
MalI GATC 2 cut(s) 234, 312
MboI GATC 2 cut(s) 232, 310
MboII GAAGA 3 cut(s) 203, 213, 424
MflI RGATCY 1 cut(s) 232
MhlI GDGCHC 4 cut(s) 108, 281, 395, 440
MlyI GAGTC 1 cut(s) 57
MnlI CCTC 6 cut(s) 127, 138, 209, 229, 257, 279
Mph1103I ATGCAT 1 cut(s) 320
MseI TTAA 2 cut(s) 15, 90
MspR9I CCNGG 1 cut(s) 171
MvaI CCWGG 1 cut(s) 171
MwoI GCNNNNNNNGC 1 cut(s) 276
NcoI CCATGG 1 cut(s) 240
NdeII GATC 2 cut(s) 232, 310
NlaIII CATG 2 cut(s) 232, 244
NlaIV GGNNCC 1 cut(s) 234
NsiI ATGCAT 1 cut(s) 320
PkrI GCNGC 1 cut(s) 269
PleI GAGTC 1 cut(s) 57
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 1 cut(s) 169
PspGI CCWGG 1 cut(s) 169
PspN4I GGNNCC 1 cut(s) 234
PspPI GGNCC 1 cut(s) 244
PsrI GAACNNNNNNTAC 2 cut(s) 288, 320
PsuI RGATCY 1 cut(s) 232
RsaI GTAC 1 cut(s) 135
RsaNI GTAC 1 cut(s) 134
SacI GAGCTC 1 cut(s) 108
SaqAI TTAA 2 cut(s) 15, 90
SatI GCNGC 1 cut(s) 268
Sau3AI GATC 2 cut(s) 232, 310
Sau96I GGNCC 1 cut(s) 244
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 1 cut(s) 171
SduI GDGCHC 4 cut(s) 108, 281, 395, 440
SetI ASST 6 cut(s) 46, 102, 108, 186, 221, 290
SfaNI GCATC 1 cut(s) 305
SfcI CTRYAG 1 cut(s) 400
SmlI CTYRAG 2 cut(s) 101, 129
SmoI CTYRAG 2 cut(s) 101, 129
SnaBI TACGTA 1 cut(s) 44
SpeI ACTAGT 1 cut(s) 50
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 1 cut(s) 169
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 21
TaiI ACGT 1 cut(s) 46
TaqI TCGA 3 cut(s) 9, 281, 313
TatI WGTACW 1 cut(s) 133
Tru1I TTAA 2 cut(s) 15, 90
Tru9I TTAA 2 cut(s) 15, 90
TscAI CASTG 1 cut(s) 129
TseI GCWGC 1 cut(s) 267
TspDTI ATGAA 2 cut(s) 194, 217
TspRI CASTG 1 cut(s) 129
XspI CTAG 1 cut(s) 51
Zsp2I ATGCAT 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.