pycom05g12230

DDB1- and CUL4-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006022
Physical Location & Seq
Reverse (-)
15687637 .. 15690994
3358 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1485 bp
ATGAACACGAGGCATCGAACAAGTATCGACAAGGCTGTCGTCAATGTTTGGCAGAGGGAGGTCGGCCAGCTCTCCGCCAGAAATTTCAAGCAGCGCCTCGCCGGCTCTGAGGATCTCGTGCTCCGATTGGAAATCTATCGGAAGCTAGAGAAGCATAAAGGATGTGTGAACACTGTAAGCTTCAATGCCGATGGTGATATTCTGGTTTCGGGCTCTGATGACAGGCGGGTTATCCTATGGAATTGGGAAACTGGGAAAGCTCAGCTTTCTTTCCATTCAGGTCATAATAATAATATATTCCAAGCAAAGATCATGCCTTATACCGATGATCGCACAATTGTTACTTGTGCTGCTGATGGGCAGGTGAGACGTTCTCAAATTCTAGAGCGGGGACAAGTGAATACTTCATTGTTGGGCAGACATATGGGCCGAGCTCATAAGTTGGCCGTTGAGCCTGGGAGTCCTCATATTTTTTACACCTGTGGTGAAGATGGATTGGTGCAACATTTTGATCTGAGGACTGATGCTGCCACAGAACTTTTCACGTGCAAACCTCTTGATAATAGGAGGAGTTACATGCCATCCATCCATCTAAATGCAATTGCAATTGACCCAAGAAACCCAAATTTCTTTGCAGTGGCAGGTGCTGATGAATATGCACGACTTTTTGATATCCGTAAGTATAACTGGGATGGGTCATCTGATGTTGGCCAACCCGCAGACTTCTTTTGCCCACCACACTTGATTGATGATGTACAAGTGGGAATTACAGGATTGGCCTTCTCAGAACAGAGCGAACTTCTTGTCTCATATAATGATGAGTTCATATATCTTTTTACTCGAGATATGGGATTGGGACCTAATCCAATTCCATCCTCTCCAGTTTCTATGGGAAGTGATGCAAGTGAAACGAGTGGTGATCATCAGTGTGAAAAGTCTCCATCAACTATGGATGTTGATAAAAGATTTGTTCCTCAAGATTACAAGGGTCATATGAACCGTGAGACAGTAAAAGGTGTGAACTTCTTTGGGCCTAATTGTGAGTATGTTGTGAGCGGGTCTGATTGTGGGCGGATATTCATATGGAAGAAAAAGGGTGGGGAGCTGATTCGTGTTATGGAAGCAGATAAGCATGTGGTAAATTGCATCGAGTCTCATCCTCACACCACAGTTCTTGCAAGTAGTGGAATTGAAAATGACATAAAGATGTGGACTCCCAAGGCCAATGAGAGAGCTATTCTGCCTCAGAAAATCGAGCAGAAGAAACCGAAGGCTAGGGGCTGGATGTACCGCTTATCGTCCCCTCAGGATTTGATGATGCAACTATTTTCACTGCAAAGGCAGAGTGAAAGCCCAGATCGCACGGGGGAGAACTCATCTTCCGGTCGGGACCTTCTAGAGCTAATACTTTCATTTGAGCCAAACAATGATGATTCTTCAGGCGATGAAGGAGACGCGGCAAGCCTGGATGATTTGTTTAGTTGA

Protein Analysis

495

Amino Acids

55.41

Weight (kDa)

5.71

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 40 - 94 9.7e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 42 - 172 5.4e-09 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 43 - 178 3.8e-14 WDR5 beta-propeller domain
Beta-prop_WDR3_2nd PF25172 43 - 170 4.6e-07 WDR3 second beta-propeller domain
WD40_Gbeta PF25391 44 - 177 3.6e-07 G protein beta WD-40 repeat protein
WD40 PF00400 46 - 81 5.1e-08 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 47 - 165 1.9e-12 WDR3 first beta-propeller domain
WDR55 PF24796 47 - 123 1.2e-06 WDR55
WD40_CDC20-Fz PF24807 49 - 226 2.6e-07 CDC20/Fizzy WD40 domain
EIF3I PF24805 49 - 124 1e-05 EIF3I
Beta-prop_THOC3 PF25174 52 - 123 1.9e-10 THOC3 beta-propeller domain
WD40_Prp19 PF24814 55 - 265 1.7e-11 Prp19 WD40 domain
Beta-prop_WDR5 PF25175 317 - 404 4e-13 WDR5 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 352, 632
AasI GACNNNNNNGTC 1 cut(s) 35
Acc36I ACCTGC 2 cut(s) 352, 632
AccBSI CCGCTC 2 cut(s) 388, 1056
AccII CGCG 1 cut(s) 1457
AciI CCGC 8 cut(s) 75, 226, 388, 717, 1056, 1072, 1291, 1457
AclWI GGATC 1 cut(s) 120
AcoI YGGCCR 3 cut(s) 64, 444, 709
AcsI RAATTY 3 cut(s) 82, 378, 625
AcuI CTGAAG 1 cut(s) 1422
AcvI CACGTG 1 cut(s) 546
AfaI GTAC 2 cut(s) 756, 1289
AgsI TTSAA 3 cut(s) 88, 184, 1193
AjnI CCWGG 2 cut(s) 454, 1464
AloI GAACNNNNNNTCC 2 cut(s) 1364, 1396
AluBI AGCT 9 cut(s) 70, 145, 180, 260, 265, 434, 1105, 1235, 1402
AluI AGCT 9 cut(s) 70, 145, 180, 260, 265, 434, 1105, 1235, 1402
Alw21I GWGCWC 2 cut(s) 123, 436
Alw26I GTCTC 6 cut(s) 361, 811, 942, 998, 1158, 1446
AlwI GGATC 1 cut(s) 120
AlwNI CAGNNNCTG 1 cut(s) 647
Ama87I CYCGRG 1 cut(s) 840
AoxI GGCC 7 cut(s) 64, 427, 444, 709, 777, 1031, 1221
ApeKI GCWGC 3 cut(s) 91, 350, 527
ApoI RAATTY 3 cut(s) 82, 378, 625
ArsI GACNNNNNNTTYG 2 cut(s) 789, 821
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 4 cut(s) 427, 857, 1031, 1390
AsuHPI GGTGA 4 cut(s) 206, 376, 497, 929
AvaI CYCGRG 1 cut(s) 840
AvaII GGWCC 2 cut(s) 857, 1390
AxyI CCTNAGG 1 cut(s) 1305
BalI TGGCCA 1 cut(s) 711
BanII GRGCYC 2 cut(s) 215, 436
BauI CACGAG 2 cut(s) 7, 116
BbrPI CACGTG 1 cut(s) 546
Bbv12I GWGCWC 2 cut(s) 123, 436
BbvI GCAGC 3 cut(s) 103, 337, 514
BccI CCATC 9 cut(s) 185, 350, 485, 589, 593, 597, 686, 880, 949
BceAI ACGGC 1 cut(s) 431
BciT130I CCWGG 2 cut(s) 456, 1466
BclI TGATCA 1 cut(s) 919
BcoDI GTCTC 6 cut(s) 361, 811, 942, 998, 1158, 1446
BfaI CTAG 4 cut(s) 146, 383, 1275, 1397
BfoI RGCGCY 1 cut(s) 97
BfuAI ACCTGC 2 cut(s) 352, 632
BglI GCCNNNNNGGC 1 cut(s) 102
BisI GCNGC 4 cut(s) 92, 351, 528, 1458
BlpI GCTNAGC 1 cut(s) 261
BlsI GCNGC 4 cut(s) 93, 352, 529, 1459
Bme1390I CCNGG 2 cut(s) 456, 1466
Bme18I GGWCC 2 cut(s) 857, 1390
BmeT110I CYCGRG 1 cut(s) 840
BmgT120I GGNCC 4 cut(s) 427, 857, 1031, 1390
BmiI GGNNCC 2 cut(s) 858, 1391
BmrFI CCNGG 2 cut(s) 456, 1466
BmrI ACTGGG 2 cut(s) 261, 697
BmsI GCATC 5 cut(s) 22, 514, 889, 1155, 1308
BmuI ACTGGG 2 cut(s) 261, 697
BplI GAGNNNNNCTC 2 cut(s) 358, 390
BpmI CTGGAG 1 cut(s) 864
Bpu1102I GCTNAGC 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 960
BsaAI YACGTR 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 455, 1218
BsaWI WCCGGW 1 cut(s) 1382
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 3 cut(s) 256, 692, 881
Bse21I CCTNAGG 1 cut(s) 1305
BseBI CCWGG 2 cut(s) 456, 1466
BseDI CCNNGG 2 cut(s) 455, 1218
BseGI GGATG 9 cut(s) 167, 581, 585, 697, 872, 958, 1156, 1290, 1474
BseMII CTCAG 6 cut(s) 99, 275, 506, 798, 1259, 1319
BseNI ACTGG 3 cut(s) 256, 692, 881
BseRI GAGGAG 1 cut(s) 583
BseXI GCAGC 3 cut(s) 103, 337, 514
Bsh1236I CGCG 1 cut(s) 1457
Bsh1285I CGRYCG 1 cut(s) 1387
BshFI GGCC 7 cut(s) 66, 429, 446, 711, 779, 1033, 1223
BsiEI CGRYCG 1 cut(s) 1387
BsiHKAI GWGCWC 2 cut(s) 123, 436
BsiHKCI CYCGRG 1 cut(s) 840
BsiSI CCGG 2 cut(s) 102, 1383
BslFI GGGAC 4 cut(s) 405, 870, 1285, 1403
BsmAI GTCTC 6 cut(s) 361, 811, 942, 998, 1158, 1446
BsmBI CGTCTC 2 cut(s) 361, 1446
BsmFI GGGAC 4 cut(s) 405, 870, 1285, 1403
BsnI GGCC 7 cut(s) 66, 429, 446, 711, 779, 1033, 1223
BsoBI CYCGRG 1 cut(s) 840
Bsp1286I GDGCHC 3 cut(s) 123, 215, 436
Bsp1407I TGTACA 1 cut(s) 754
Bsp143I GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
Bsp1720I GCTNAGC 1 cut(s) 261
BspACI CCGC 8 cut(s) 75, 226, 388, 717, 1056, 1072, 1291, 1457
BspANI GGCC 7 cut(s) 66, 429, 446, 711, 779, 1033, 1223
BspCNI CTCAG 6 cut(s) 100, 274, 507, 797, 1258, 1318
BspFNI CGCG 1 cut(s) 1457
BspLI GGNNCC 2 cut(s) 858, 1391
BspMI ACCTGC 2 cut(s) 352, 632
BspPI GGATC 1 cut(s) 120
BsrBI CCGCTC 2 cut(s) 388, 1056
BsrFI RCCGGY 1 cut(s) 101
BsrGI TGTACA 1 cut(s) 754
BsrI ACTGG 3 cut(s) 256, 692, 881
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 455, 1218
BssMI GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
BssSI CACGAG 2 cut(s) 7, 116
BssT1I CCWWGG 1 cut(s) 1218
Bst2BI CACGAG 2 cut(s) 7, 116
Bst2UI CCWGG 2 cut(s) 456, 1466
Bst4CI ACNGT 4 cut(s) 175, 1001, 1009, 1171
BstAUI TGTACA 1 cut(s) 754
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 3 cut(s) 68, 103, 1462
BstDEI CTNAG 6 cut(s) 108, 261, 515, 784, 1245, 1305
BstF5I GGATG 9 cut(s) 167, 581, 585, 697, 872, 958, 1156, 1290, 1474
BstFNI CGCG 1 cut(s) 1457
BstH2I RGCGCY 1 cut(s) 97
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 6 cut(s) 115, 312, 331, 514, 922, 1360
BstMAI GTCTC 6 cut(s) 361, 811, 942, 998, 1158, 1446
BstMBI GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
BstMCI CGRYCG 1 cut(s) 1387
BstMWI GCNNNNNNNGC 3 cut(s) 102, 151, 1359
BstNI CCWGG 2 cut(s) 456, 1466
BstNSI RCATGY 2 cut(s) 580, 1136
BstSCI CCNGG 2 cut(s) 454, 1464
BstUI CGCG 1 cut(s) 1457
BstV1I GCAGC 3 cut(s) 103, 337, 514
BstX2I RGATCY 1 cut(s) 112
BstYI RGATCY 1 cut(s) 112
Bsu36I CCTNAGG 1 cut(s) 1305
BsuRI GGCC 7 cut(s) 66, 429, 446, 711, 779, 1033, 1223
BtgZI GCGATG 1 cut(s) 1458
BtsCI GGATG 9 cut(s) 167, 581, 585, 697, 872, 958, 1156, 1290, 1474
BtsI GCAGTG 2 cut(s) 642, 1331
BtsIMutI CAGTG 4 cut(s) 171, 642, 932, 1331
BveI ACCTGC 2 cut(s) 352, 632
Cac8I GCNNGC 3 cut(s) 68, 103, 1462
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 96
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 4 cut(s) 427, 857, 1031, 1390
CseI GACGC 1 cut(s) 1463
Csp6I GTAC 2 cut(s) 755, 1288
CspCI CAANNNNNGTGG 4 cut(s) 723, 758, 1156, 1191
CviAII CATG 3 cut(s) 313, 577, 1133
CviQI GTAC 2 cut(s) 755, 1288
DdeI CTNAG 6 cut(s) 108, 261, 515, 784, 1245, 1305
DpnI GATC 6 cut(s) 114, 311, 330, 513, 921, 1359
DpnII GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
DrdI GACNNNNNNGTC 1 cut(s) 35
DseDI GACNNNNNNGTC 1 cut(s) 35
EaeI YGGCCR 3 cut(s) 64, 444, 709
EciI GGCGGA 2 cut(s) 64, 1087
Ecl136II GAGCTC 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 2 cut(s) 215, 436
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 857, 1390
Eco53kI GAGCTC 1 cut(s) 434
Eco57I CTGAAG 1 cut(s) 1422
Eco72I CACGTG 1 cut(s) 546
Eco81I CCTNAGG 1 cut(s) 1305
Eco88I CYCGRG 1 cut(s) 840
EcoICRI GAGCTC 1 cut(s) 434
EcoO109I RGGNCCY 2 cut(s) 857, 1390
EcoRII CCWGG 2 cut(s) 454, 1464
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 1218
EcoT38I GRGCYC 2 cut(s) 215, 436
ErhI CCWWGG 1 cut(s) 1218
Esp3I CGTCTC 2 cut(s) 361, 1446
FaeI CATG 3 cut(s) 316, 580, 1136
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 4 cut(s) 405, 870, 1285, 1403
FatI CATG 3 cut(s) 312, 576, 1132
FauI CCCGC 4 cut(s) 219, 381, 724, 1049
FauNDI CATATG 3 cut(s) 423, 993, 1082
FbaI TGATCA 1 cut(s) 919
Fnu4HI GCNGC 4 cut(s) 92, 351, 528, 1458
FokI GGATG 9 cut(s) 174, 568, 572, 704, 859, 965, 1143, 1297, 1481
FriOI GRGCYC 2 cut(s) 215, 436
Fsp4HI GCNGC 4 cut(s) 92, 351, 528, 1458
FspBI CTAG 4 cut(s) 146, 383, 1275, 1397
GlaI GCGC 1 cut(s) 95
GluI GCNGC 4 cut(s) 92, 351, 528, 1458
GsuI CTGGAG 1 cut(s) 864
HaeII RGCGCY 1 cut(s) 97
HaeIII GGCC 7 cut(s) 66, 429, 446, 711, 779, 1033, 1223
HapII CCGG 2 cut(s) 102, 1383
HgaI GACGC 1 cut(s) 1463
HhaI GCGC 1 cut(s) 96
Hin1II CATG 3 cut(s) 316, 580, 1136
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 5 cut(s) 460, 1108, 1151, 1213, 1433
HpaII CCGG 2 cut(s) 102, 1383
HphI GGTGA 4 cut(s) 206, 376, 497, 929
Hpy166II GTNNAC 3 cut(s) 169, 1021, 1212
Hpy188I TCNGA 9 cut(s) 109, 125, 141, 217, 516, 703, 787, 1063, 1248
Hpy188III TCNNGA 7 cut(s) 383, 557, 842, 977, 1307, 1388, 1397
Hpy8I GTNNAC 3 cut(s) 169, 1021, 1212
HpyAV CCTTC 4 cut(s) 790, 1264, 1403, 1442
HpyCH4III ACNGT 4 cut(s) 175, 1001, 1009, 1171
HpyCH4IV ACGT 2 cut(s) 370, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 151, 1359
HpyF3I CTNAG 6 cut(s) 108, 261, 515, 784, 1245, 1305
HpySE526I ACGT 2 cut(s) 370, 545
Hsp92II CATG 3 cut(s) 316, 580, 1136
HspAI GCGC 1 cut(s) 94
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 919
Kzo9I GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
LmnI GCTCC 2 cut(s) 126, 1102
Lsp1109I GCAGC 3 cut(s) 103, 337, 514
LweI GCATC 5 cut(s) 22, 514, 889, 1155, 1308
MaeI CTAG 4 cut(s) 146, 383, 1275, 1397
MaeII ACGT 2 cut(s) 370, 545
MaeIII GTNAC 2 cut(s) 340, 572
MalI GATC 6 cut(s) 114, 311, 330, 513, 921, 1359
MbiI CCGCTC 2 cut(s) 388, 1056
MboI GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
MboII GAAGA 5 cut(s) 500, 1099, 1273, 1371, 1428
MfeI CAATTG 3 cut(s) 336, 600, 606
MflI RGATCY 1 cut(s) 112
MhlI GDGCHC 3 cut(s) 123, 215, 436
MlsI TGGCCA 1 cut(s) 711
MluNI TGGCCA 1 cut(s) 711
MlyI GAGTC 3 cut(s) 469, 1160, 1207
Mox20I TGGCCA 1 cut(s) 711
MroNI GCCGGC 1 cut(s) 101
MscI TGGCCA 1 cut(s) 711
MslI CAYNNNNRTG 3 cut(s) 594, 927, 1205
Msp20I TGGCCA 1 cut(s) 711
MspI CCGG 2 cut(s) 102, 1383
MspR9I CCNGG 2 cut(s) 456, 1466
MunI CAATTG 3 cut(s) 336, 600, 606
MvaI CCWGG 2 cut(s) 456, 1466
MvnI CGCG 1 cut(s) 1457
MwoI GCNNNNNNNGC 3 cut(s) 102, 151, 1359
NaeI GCCGGC 1 cut(s) 103
NdeI CATATG 3 cut(s) 423, 993, 1082
NdeII GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 3 cut(s) 316, 580, 1136
NlaIV GGNNCC 2 cut(s) 858, 1391
NmeAIII GCCGAG 1 cut(s) 455
NspI RCATGY 2 cut(s) 580, 1136
PaeR7I CTCGAG 1 cut(s) 840
PaqCI CACCTGC 2 cut(s) 352, 632
PdiI GCCGGC 1 cut(s) 103
PfeI GAWTC 2 cut(s) 1108, 1433
PkrI GCNGC 4 cut(s) 93, 352, 529, 1459
PleI GAGTC 3 cut(s) 468, 1159, 1207
PmaCI CACGTG 1 cut(s) 546
PmlI CACGTG 1 cut(s) 546
PpsI GAGTC 3 cut(s) 468, 1159, 1207
Ppu21I YACGTR 1 cut(s) 546
PpuMI RGGWCCY 2 cut(s) 857, 1390
Psp124BI GAGCTC 1 cut(s) 436
Psp5II RGGWCCY 2 cut(s) 857, 1390
Psp6I CCWGG 2 cut(s) 454, 1464
PspCI CACGTG 1 cut(s) 546
PspGI CCWGG 2 cut(s) 454, 1464
PspN4I GGNNCC 2 cut(s) 858, 1391
PspPI GGNCC 4 cut(s) 427, 857, 1031, 1390
PspPPI RGGWCCY 2 cut(s) 857, 1390
PstNI CAGNNNCTG 1 cut(s) 647
PsuI RGATCY 1 cut(s) 112
RsaI GTAC 2 cut(s) 756, 1289
RsaNI GTAC 2 cut(s) 755, 1288
RseI CAYNNNNRTG 3 cut(s) 594, 927, 1205
SacI GAGCTC 1 cut(s) 436
SatI GCNGC 4 cut(s) 92, 351, 528, 1458
Sau3AI GATC 6 cut(s) 112, 309, 328, 511, 919, 1357
Sau96I GGNCC 4 cut(s) 427, 857, 1031, 1390
SchI GAGTC 3 cut(s) 469, 1160, 1207
ScrFI CCNGG 2 cut(s) 456, 1466
SduI GDGCHC 3 cut(s) 123, 215, 436
SfaNI GCATC 5 cut(s) 22, 514, 889, 1155, 1308
Sfr274I CTCGAG 1 cut(s) 840
SinI GGWCC 2 cut(s) 857, 1390
SlaI CTCGAG 1 cut(s) 840
SmiMI CAYNNNNRTG 3 cut(s) 594, 927, 1205
SmlI CTYRAG 2 cut(s) 840, 975
SmoI CTYRAG 2 cut(s) 840, 975
SsiI CCGC 8 cut(s) 75, 226, 388, 717, 1056, 1072, 1291, 1457
SspMI CTAG 4 cut(s) 146, 383, 1275, 1397
SstI GAGCTC 1 cut(s) 436
StyD4I CCNGG 2 cut(s) 454, 1464
StyI CCWWGG 1 cut(s) 1218
TaaI ACNGT 4 cut(s) 175, 1001, 1009, 1171
TaiI ACGT 2 cut(s) 373, 548
TaqI TCGA 5 cut(s) 16, 27, 841, 1149, 1254
TatI WGTACW 1 cut(s) 754
TauI GCSGC 1 cut(s) 1460
TfiI GAWTC 2 cut(s) 1108, 1433
TscAI CASTG 4 cut(s) 178, 642, 932, 1338
TseI GCWGC 3 cut(s) 91, 350, 527
TspDTI ATGAA 8 cut(s) 17, 396, 666, 814, 1010, 1069, 1401, 1461
TspGWI ACGGA 1 cut(s) 665
TspRI CASTG 4 cut(s) 178, 642, 932, 1338
VpaK11BI GGWCC 2 cut(s) 857, 1390
XapI RAATTY 3 cut(s) 82, 378, 625
XbaI TCTAGA 2 cut(s) 382, 1396
XceI RCATGY 2 cut(s) 580, 1136
XhoI CTCGAG 1 cut(s) 840
XspI CTAG 4 cut(s) 146, 383, 1275, 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.