pycom05g13440

protein phosphatase 2C 27

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002573
Physical Location & Seq
Reverse (-)
16993607 .. 16995219
1613 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
ATGCGAAAAGGAGTGTTTGAAAGGGTATACTTGAGGTCCAGTTATTTATCCGGTGGTGGATTATGTTTTGGGGAGAAAATGGCTGCAGGGACTGATTACCCGCAGGCACTTTCCATGTTGGATGGCAGTTATAGAAAGGAGAACGTTACTGTTACAAGAGACGGGAAATCAAGTACTTTTGAGAACTTGAAGCAACCGAACATTGGCAGACCTCCTCGTCACCTCTCAGCTATTAGGTGTTGCGTAAGCTCTGCCCAGTTGGCTCCTGCAGCTGAAGTGGAGTTGGATGTTGGGATTGTTAGCTCAAAGTCTCCATCAGATAGTAACTCAGCTTTTGTACCCGTTTTTCACTCTGGAAGCTGCTCGGAGATTGGACCGAAGCCATATATGGAGGATGGGCATGTTTGTATAGACAATATTCTTGATCATCTAGGTGCAACTGCGAACTTCACTTCTCCTGGAGCATTCTATGTGATTTTTTCTTCTTTTTTTTTTTGGGTTGTTGGAAACAAATTTTTGAACAATTCTATCTTATTATGCGAAGTCCACAGGACGATGCTAATTGCAAATGCTGGAGATTGCCGAGCTGTGCTGGGAAGAAGAGGTCGGGCAATTGAGCTTTCCAAAGACCATAAACCCAATTGTGCCTCCGAAAGAGTGAGAATCGAGAAACTGGGAGGCATGGTGTATGATGGCTACCTCAACGGTCAATTATCTGTGGCACGAGCTCTTGGGGACTGGCACATGAAAGGCTCCAAAGGTTCCTTGTGTCCCTTGAGTGCAGAGCCAGAGCTCGAGGAGACAATTCTGAGGGAGGAAGACGAATTCTTAATCCTAGGCTGTGATGGGTTGTGGGATGTGATGAGCAGCCAGTGTGCAGTTACGATGGTAAGGAAAGAACTAATGATCCACAATGATCCCGAGAGATGCTCAAGAGAGCTGGTTCGGGAGGCACTCAAGCGCAATACGTGTGATAACCTGACGGTTGTTGTGGTATGCTTCTCGGCAGATCCACCCCCGTTGCTTGAGATACCAAAGTTAAAATTCAAGAGGAGTTTATCGGCGGAAGGCCTGAATCTTCTGCAGGAGGTACTAGATAGTAAATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PP2C

Protein Analysis

370

Amino Acids

40.55

Weight (kDa)

6.27

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 179 - 326 4.1e-53 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 216
AccI GTMKAC 1 cut(s) 27
AciI CCGC 2 cut(s) 101, 1064
AclI AACGTT 1 cut(s) 144
AclWI GGATC 3 cut(s) 901, 911, 1004
AcsI RAATTY 3 cut(s) 512, 824, 1043
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 3 cut(s) 175, 339, 1092
AfiI CCNNNNNNNGG 1 cut(s) 203
AflIII ACRYGT 1 cut(s) 968
AgsI TTSAA 4 cut(s) 20, 190, 520, 1048
AjnI CCWGG 1 cut(s) 457
AloI GAACNNNNNNTCC 2 cut(s) 891, 923
Alw21I GWGCWC 2 cut(s) 730, 795
Alw26I GTCTC 3 cut(s) 153, 315, 794
AlwI GGATC 3 cut(s) 901, 911, 1004
AlwNI CAGNNNCTG 1 cut(s) 92
Ama87I CYCGRG 2 cut(s) 794, 920
AoxI GGCC 1 cut(s) 1069
ApeKI GCWGC 4 cut(s) 83, 269, 360, 867
ApoI RAATTY 3 cut(s) 512, 824, 1043
AspA2I CCTAGG 1 cut(s) 835
AspLEI GCGC 1 cut(s) 963
AspS9I GGNCC 2 cut(s) 36, 374
AsuHPI GGTGA 1 cut(s) 212
AvaI CYCGRG 2 cut(s) 794, 920
AvaII GGWCC 2 cut(s) 36, 374
AvrII CCTAGG 1 cut(s) 835
BaeI ACNNNNGTAYC 2 cut(s) 1022, 1055
BanII GRGCYC 2 cut(s) 730, 795
BauI CACGAG 1 cut(s) 723
BbsI GAAGAC 1 cut(s) 825
Bbv12I GWGCWC 2 cut(s) 730, 795
BbvI GCAGC 4 cut(s) 70, 281, 347, 879
BccI CCATC 6 cut(s) 116, 322, 389, 686, 839, 880
BciT130I CCWGG 1 cut(s) 459
BclI TGATCA 1 cut(s) 424
BcoDI GTCTC 3 cut(s) 153, 315, 794
BfaI CTAG 3 cut(s) 431, 836, 1094
BfmI CTRYAG 3 cut(s) 84, 267, 1082
BglI GCCNNNNNGGC 1 cut(s) 260
BisI GCNGC 4 cut(s) 84, 270, 361, 868
BlnI CCTAGG 1 cut(s) 835
BlsI GCNGC 4 cut(s) 85, 271, 362, 869
BmcAI AGTACT 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 459
Bme18I GGWCC 2 cut(s) 36, 374
BmeT110I CYCGRG 2 cut(s) 794, 920
BmgT120I GGNCC 2 cut(s) 36, 374
BmiI GGNNCC 3 cut(s) 264, 754, 763
BmrFI CCNGG 1 cut(s) 459
BmrI ACTGGG 2 cut(s) 250, 683
BmsI GCATC 2 cut(s) 546, 917
BmuI ACTGGG 2 cut(s) 250, 683
BpiI GAAGAC 1 cut(s) 825
BplI GAGNNNNNCTC 2 cut(s) 914, 946
BpmI CTGGAG 2 cut(s) 480, 594
BpuEI CTTGAG 5 cut(s) 52, 796, 916, 941, 1046
BsaAI YACGTR 1 cut(s) 969
BsaJI CCNNGG 1 cut(s) 835
BsaWI WCCGGW 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 669, 699
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 5 cut(s) 39, 256, 678, 743, 871
BseBI CCWGG 1 cut(s) 459
BseDI CCNNGG 1 cut(s) 835
BseGI GGATG 4 cut(s) 127, 292, 400, 862
BseLI CCNNNNNNNGG 1 cut(s) 203
BseMII CTCAG 3 cut(s) 240, 342, 800
BseNI ACTGG 5 cut(s) 39, 256, 678, 743, 871
BseRI GAGGAG 3 cut(s) 204, 812, 1066
BseXI GCAGC 4 cut(s) 70, 281, 347, 879
BseYI CCCAGC 1 cut(s) 592
BsgI GTGCAG 2 cut(s) 801, 897
BshFI GGCC 1 cut(s) 1071
BsiHKAI GWGCWC 2 cut(s) 730, 795
BsiHKCI CYCGRG 2 cut(s) 794, 920
BsiSI CCGG 1 cut(s) 51
BslFI GGGAC 3 cut(s) 103, 749, 756
BslI CCNNNNNNNGG 1 cut(s) 203
BsmAI GTCTC 3 cut(s) 153, 315, 794
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 3 cut(s) 103, 749, 756
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 1071
BsoBI CYCGRG 2 cut(s) 794, 920
Bsp1286I GDGCHC 2 cut(s) 730, 795
Bsp143I GATC 4 cut(s) 424, 906, 916, 1009
BspACI CCGC 2 cut(s) 101, 1064
BspANI GGCC 1 cut(s) 1071
BspCNI CTCAG 3 cut(s) 239, 341, 801
BspHI TCATGA 1 cut(s) 1106
BspLI GGNNCC 3 cut(s) 264, 754, 763
BspMAI CTGCAG 3 cut(s) 88, 271, 1086
BspPI GGATC 3 cut(s) 901, 911, 1004
BsrI ACTGG 5 cut(s) 39, 256, 678, 743, 871
BssECI CCNNGG 1 cut(s) 835
BssMI GATC 4 cut(s) 424, 906, 916, 1009
BssNAI GTATAC 1 cut(s) 28
BssSI CACGAG 1 cut(s) 723
BssT1I CCWWGG 1 cut(s) 835
Bst1107I GTATAC 1 cut(s) 28
Bst2BI CACGAG 1 cut(s) 723
Bst2UI CCWGG 1 cut(s) 459
Bst4CI ACNGT 3 cut(s) 151, 707, 985
Bst6I CTCTTC 1 cut(s) 595
BstBAI YACGTR 1 cut(s) 969
BstC8I GCNNGC 1 cut(s) 105
BstDEI CTNAG 3 cut(s) 226, 328, 809
BstF5I GGATG 4 cut(s) 127, 292, 400, 862
BstHHI GCGC 1 cut(s) 963
BstKTI GATC 4 cut(s) 427, 909, 919, 1012
BstMAI GTCTC 3 cut(s) 153, 315, 794
BstMBI GATC 4 cut(s) 424, 906, 916, 1009
BstMWI GCNNNNNNNGC 2 cut(s) 260, 269
BstNI CCWGG 1 cut(s) 459
BstNSI RCATGY 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 457
BstSFI CTRYAG 3 cut(s) 84, 267, 1082
BstV1I GCAGC 4 cut(s) 70, 281, 347, 879
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 1 cut(s) 1009
BstYI RGATCY 1 cut(s) 1009
BstZ17I GTATAC 1 cut(s) 28
BsuRI GGCC 1 cut(s) 1071
BtsCI GGATG 4 cut(s) 127, 292, 400, 862
BtsIMutI CAGTG 1 cut(s) 878
Cac8I GCNNGC 1 cut(s) 105
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 1106
CfoI GCGC 1 cut(s) 963
Cfr13I GGNCC 2 cut(s) 36, 374
Csp6I GTAC 3 cut(s) 174, 338, 1091
CspCI CAANNNNNGTGG 2 cut(s) 1002, 1037
CviAII CATG 5 cut(s) 115, 401, 682, 745, 1107
CviQI GTAC 3 cut(s) 174, 338, 1091
DdeI CTNAG 3 cut(s) 226, 328, 809
DpnI GATC 4 cut(s) 426, 908, 918, 1011
DpnII GATC 4 cut(s) 424, 906, 916, 1009
DrdI GACNNNNNNGTC 1 cut(s) 216
DseDI GACNNNNNNGTC 1 cut(s) 216
Eam1104I CTCTTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 595
EciI GGCGGA 1 cut(s) 1079
Ecl136II GAGCTC 2 cut(s) 728, 793
Eco130I CCWWGG 1 cut(s) 835
Eco147I AGGCCT 1 cut(s) 1071
Eco24I GRGCYC 2 cut(s) 730, 795
Eco47I GGWCC 2 cut(s) 36, 374
Eco53kI GAGCTC 2 cut(s) 728, 793
Eco57I CTGAAG 1 cut(s) 294
Eco88I CYCGRG 2 cut(s) 794, 920
EcoICRI GAGCTC 2 cut(s) 728, 793
EcoRI GAATTC 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 835
EcoT38I GRGCYC 2 cut(s) 730, 795
ErhI CCWWGG 1 cut(s) 835
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 5 cut(s) 118, 404, 685, 748, 1110
FaqI GGGAC 3 cut(s) 103, 749, 756
FatI CATG 5 cut(s) 114, 400, 681, 744, 1106
FauI CCCGC 1 cut(s) 108
FbaI TGATCA 1 cut(s) 424
FblI GTMKAC 1 cut(s) 27
Fnu4HI GCNGC 4 cut(s) 84, 270, 361, 868
FokI GGATG 4 cut(s) 134, 299, 407, 869
FriOI GRGCYC 2 cut(s) 730, 795
Fsp4HI GCNGC 4 cut(s) 84, 270, 361, 868
FspBI CTAG 3 cut(s) 431, 836, 1094
GlaI GCGC 1 cut(s) 962
GluI GCNGC 4 cut(s) 84, 270, 361, 868
GsaI CCCAGC 1 cut(s) 596
GsuI CTGGAG 2 cut(s) 480, 594
HaeIII GGCC 1 cut(s) 1071
HapII CCGG 1 cut(s) 51
HhaI GCGC 1 cut(s) 963
Hin1II CATG 5 cut(s) 118, 404, 685, 748, 1110
Hin6I GCGC 1 cut(s) 961
HinP1I GCGC 1 cut(s) 961
HinfI GANTC 2 cut(s) 663, 1075
HpaII CCGG 1 cut(s) 51
HphI GGTGA 1 cut(s) 212
Hpy166II GTNNAC 2 cut(s) 28, 547
Hpy188I TCNGA 4 cut(s) 319, 367, 652, 810
Hpy188III TCNNGA 8 cut(s) 354, 422, 667, 920, 933, 947, 1048, 1107
Hpy8I GTNNAC 2 cut(s) 28, 547
HpyAV CCTTC 1 cut(s) 1061
HpyCH4III ACNGT 3 cut(s) 151, 707, 985
HpyCH4IV ACGT 2 cut(s) 144, 968
HpyCH4V TGCA 7 cut(s) 86, 269, 437, 566, 782, 878, 1084
HpyF10VI GCNNNNNNNGC 2 cut(s) 260, 269
HpyF3I CTNAG 3 cut(s) 226, 328, 809
HpySE526I ACGT 2 cut(s) 144, 968
Hsp92II CATG 5 cut(s) 118, 404, 685, 748, 1110
HspAI GCGC 1 cut(s) 961
Ksp22I TGATCA 1 cut(s) 424
Kzo9I GATC 4 cut(s) 424, 906, 916, 1009
LmnI GCTCC 3 cut(s) 268, 461, 758
Lsp1109I GCAGC 4 cut(s) 70, 281, 347, 879
LweI GCATC 2 cut(s) 546, 917
MaeI CTAG 3 cut(s) 431, 836, 1094
MaeII ACGT 2 cut(s) 144, 968
MaeIII GTNAC 5 cut(s) 145, 151, 218, 323, 880
MalI GATC 4 cut(s) 426, 908, 918, 1011
MboI GATC 4 cut(s) 424, 906, 916, 1009
MboII GAAGA 5 cut(s) 474, 609, 612, 830, 1070
MfeI CAATTG 2 cut(s) 612, 640
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 2 cut(s) 730, 795
MluCI AATT 9 cut(s) 512, 523, 561, 612, 640, 710, 804, 824, 1043
MmeI TCCRAC 3 cut(s) 99, 264, 484
MseI TTAA 2 cut(s) 830, 1040
MslI CAYNNNNRTG 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 459
MunI CAATTG 2 cut(s) 612, 640
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 459
MwoI GCNNNNNNNGC 2 cut(s) 260, 269
NdeII GATC 4 cut(s) 424, 906, 916, 1009
NlaIII CATG 5 cut(s) 118, 404, 685, 748, 1110
NlaIV GGNNCC 3 cut(s) 264, 754, 763
NmeAIII GCCGAG 2 cut(s) 608, 983
NmuCI GTSAC 1 cut(s) 218
NspI RCATGY 1 cut(s) 404
PaeR7I CTCGAG 1 cut(s) 794
PagI TCATGA 1 cut(s) 1106
PceI AGGCCT 1 cut(s) 1071
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 663, 1075
PfoI TCCNGGA 1 cut(s) 457
PkrI GCNGC 4 cut(s) 85, 271, 362, 869
Ppu21I YACGTR 1 cut(s) 969
Psp124BI GAGCTC 2 cut(s) 730, 795
Psp1406I AACGTT 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 592
PspGI CCWGG 1 cut(s) 457
PspN4I GGNNCC 3 cut(s) 264, 754, 763
PspPI GGNCC 2 cut(s) 36, 374
PspXI VCTCGAGB 1 cut(s) 794
PstI CTGCAG 3 cut(s) 88, 271, 1086
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 1 cut(s) 1009
PvuII CAGCTG 1 cut(s) 272
RsaI GTAC 3 cut(s) 175, 339, 1092
RsaNI GTAC 3 cut(s) 174, 338, 1091
RseI CAYNNNNRTG 1 cut(s) 432
SacI GAGCTC 2 cut(s) 730, 795
SaqAI TTAA 2 cut(s) 830, 1040
SatI GCNGC 4 cut(s) 84, 270, 361, 868
Sau3AI GATC 4 cut(s) 424, 906, 916, 1009
Sau96I GGNCC 2 cut(s) 36, 374
ScaI AGTACT 1 cut(s) 175
ScrFI CCNGG 1 cut(s) 459
SduI GDGCHC 2 cut(s) 730, 795
SfaNI GCATC 2 cut(s) 546, 917
SfcI CTRYAG 3 cut(s) 84, 267, 1082
Sfr274I CTCGAG 1 cut(s) 794
SinI GGWCC 2 cut(s) 36, 374
SlaI CTCGAG 1 cut(s) 794
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 6 cut(s) 31, 775, 794, 931, 956, 1025
SmoI CTYRAG 6 cut(s) 31, 775, 794, 931, 956, 1025
Sse9I AATT 9 cut(s) 512, 523, 561, 612, 640, 710, 804, 824, 1043
SseBI AGGCCT 1 cut(s) 1071
SsiI CCGC 2 cut(s) 101, 1064
SspI AATATT 1 cut(s) 418
SspMI CTAG 3 cut(s) 431, 836, 1094
SstI GAGCTC 2 cut(s) 730, 795
StuI AGGCCT 1 cut(s) 1071
StyD4I CCNGG 1 cut(s) 457
StyI CCWWGG 1 cut(s) 835
TaaI ACNGT 3 cut(s) 151, 707, 985
TaiI ACGT 2 cut(s) 147, 971
TaqI TCGA 2 cut(s) 666, 795
TaqII GACCGA 1 cut(s) 391
TasI AATT 9 cut(s) 512, 523, 561, 612, 640, 710, 804, 824, 1043
TatI WGTACW 1 cut(s) 173
TfiI GAWTC 2 cut(s) 663, 1075
Tru1I TTAA 2 cut(s) 830, 1040
Tru9I TTAA 2 cut(s) 830, 1040
TscAI CASTG 1 cut(s) 878
TseFI GTSAC 1 cut(s) 218
TseI GCWGC 4 cut(s) 83, 269, 360, 867
Tsp45I GTSAC 1 cut(s) 218
TspDTI ATGAA 1 cut(s) 761
TspRI CASTG 1 cut(s) 878
VpaK11BI GGWCC 2 cut(s) 36, 374
XapI RAATTY 3 cut(s) 512, 824, 1043
XceI RCATGY 1 cut(s) 404
XhoI CTCGAG 1 cut(s) 794
XmaJI CCTAGG 1 cut(s) 835
XmiI GTMKAC 1 cut(s) 27
XspI CTAG 3 cut(s) 431, 836, 1094
ZrmI AGTACT 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.