pycom05g21620

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0004070
Physical Location & Seq
Reverse (-)
24199460 .. 24200607
1148 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21620.1

Sequence Viewer

Length: 1041 bp
ATGAAGTTGTGTATTTCTACACTGTCTGTTGTTCTTGTTCTGTTGGAGCTGTTCGTGATCGATGTTGTATCGGTGACTAAGTTACCTATAACGGCAGCTCTCAACGTAAAAAAGCCTCGGGCCAAAGCTGTTCGTGCCGAGGATCTTCTGTCTTTTGATCACTATGTTAAAACATGCCCGCAAGCTGAGGGAATCATTCAGCAGAAAGTCGGAGATTGGACTCAAAAGGATTTCACTTTGGCTGCTAGCATCATTCGCTTGCATTTCCACGACTGCGTTGTGAGAGGCTGCGACGCATCGATTCTGCTTAACCATCGAGACAGCGAGAGGAGGGCATTTGCTAGCAGGACACTTAGAGGTTTTGAAGTAATCGACGATATCAAGGCTGAGCTTGAGAGACAGTGTCCTAAAACCGTCTCATGTGCTGACATTCTCACCGCTGCAACTAGAGATGCCACAATCATAGCTGGAGGTCCATTTTGGGAAGTCCCCTTTGGGCGCAAAGATGGTAAATTTTCAATCCTCAAGGAAGCTGACAGTGTTCCTCAGGGATACGAGAACATCACTCAATTGATCGATTTTTTCCAAGCGCGCGGCTTGAACATGCTTGATCTGGTCACCCTCTCCGGGGCACACACCATTGGCAGAAGTTCTTGCTACGCCTTCAAGCACAGGCTCTCAAATTTCAATGGAACAAGAAAGCCCGACCCTTCGCTTAACTCCATGTACTTAAATAATTTCTTGAAGAAGAAATGCAAAAACGACTTGGACTTGGTTTATCTCGACGCCATAACTCCAAAGACATTCGATACAATGTACTACTCGAATCTTCACAAGAAGTTGGGATTGCTGTCAACTGATCAGCTGCTCAATTCAGACGAGAGAACCGGTCCTTTTGTTGCTGCATTGGCGTCCCAGCCGTCGCTTTTCGAAGGCCAGTTTTCGGTGTCAATGGTGAAGCTTGGAAATGTTCAAGTTCTAACAAGAAGCAATGAAGGAGAAATCCGAGTGAACTGCAATTTTGTCAATACTAAAAAATGA

Protein Analysis

347

Amino Acids

38.6

Weight (kDa)

8.87

Isoelectric Point (pI)

25.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 67 - 305 7.6e-76 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 592, 594
AciI CCGC 3 cut(s) 179, 438, 594
AclWI GGATC 1 cut(s) 150
AcsI RAATTY 2 cut(s) 512, 682
AcyI GRCGYC 2 cut(s) 786, 911
AfaI GTAC 2 cut(s) 728, 818
AfiI CCNNNNNNNGG 3 cut(s) 627, 628, 943
AgeI ACCGGT 1 cut(s) 887
AgsI TTSAA 7 cut(s) 365, 519, 601, 667, 688, 745, 974
AjuI GAANNNNNNNTTGG 2 cut(s) 477, 509
AluBI AGCT 9 cut(s) 49, 98, 128, 185, 391, 467, 533, 865, 961
AluI AGCT 9 cut(s) 49, 98, 128, 185, 391, 467, 533, 865, 961
Alw26I GTCTC 3 cut(s) 312, 391, 421
AlwI GGATC 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 117
AoxI GGCC 2 cut(s) 120, 934
ApeKI GCWGC 6 cut(s) 95, 242, 288, 440, 865, 902
ApoI RAATTY 2 cut(s) 512, 682
AsiGI ACCGGT 1 cut(s) 887
AspLEI GCGC 3 cut(s) 501, 592, 594
AspS9I GGNCC 3 cut(s) 120, 473, 890
AsuC2I CCSGG 1 cut(s) 628
AsuHPI GGTGA 4 cut(s) 85, 427, 610, 967
AsuII TTCGAA 1 cut(s) 930
AsuNHI GCTAGC 2 cut(s) 245, 341
AvaI CYCGRG 1 cut(s) 117
AvaII GGWCC 2 cut(s) 473, 890
AxyI CCTNAGG 1 cut(s) 546
BaeGI GKGCMC 1 cut(s) 634
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 6 cut(s) 107, 229, 275, 427, 852, 889
BccI CCATC 2 cut(s) 321, 500
BceAI ACGGC 2 cut(s) 108, 904
BcgI CGANNNNNNTGC 2 cut(s) 996, 1030
BciVI GTATCC 1 cut(s) 545
BclI TGATCA 2 cut(s) 157, 859
BcnI CCSGG 1 cut(s) 628
BcoDI GTCTC 3 cut(s) 312, 391, 421
BfaI CTAG 3 cut(s) 246, 342, 447
BfuI GTATCC 1 cut(s) 545
BisI GCNGC 7 cut(s) 96, 243, 289, 441, 595, 866, 903
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 7 cut(s) 97, 244, 290, 442, 596, 867, 904
Bme1390I CCNGG 1 cut(s) 628
Bme18I GGWCC 2 cut(s) 473, 890
BmeT110I CYCGRG 1 cut(s) 117
BmgT120I GGNCC 3 cut(s) 120, 473, 890
BmrFI CCNGG 1 cut(s) 628
BmsI GCATC 3 cut(s) 258, 305, 442
BmtI GCTAGC 2 cut(s) 249, 345
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 186
Bpu1102I GCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 930
BpuEI CTTGAG 2 cut(s) 413, 509
BpuMI CCSGG 1 cut(s) 628
Bsa29I ATCGAT 3 cut(s) 60, 299, 576
BsaHI GRCGYC 2 cut(s) 786, 911
BsaJI CCNNGG 3 cut(s) 116, 138, 627
BsaWI WCCGGW 1 cut(s) 887
Bsc4I CCNNNNNNNGG 3 cut(s) 627, 628, 943
Bse118I RCCGGY 1 cut(s) 887
Bse1I ACTGG 1 cut(s) 937
Bse21I CCTNAGG 1 cut(s) 546
Bse3DI GCAATG 1 cut(s) 997
BseCI ATCGAT 3 cut(s) 60, 299, 576
BseDI CCNNGG 3 cut(s) 116, 138, 627
BseLI CCNNNNNNNGG 3 cut(s) 627, 628, 943
BseMI GCAATG 1 cut(s) 997
BseMII CTCAG 3 cut(s) 177, 378, 560
BseNI ACTGG 1 cut(s) 937
BsePI GCGCGC 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 343
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 6 cut(s) 107, 229, 275, 427, 852, 889
BseYI CCCAGC 1 cut(s) 915
Bsh1236I CGCG 2 cut(s) 592, 594
BshFI GGCC 2 cut(s) 122, 936
BshTI ACCGGT 1 cut(s) 887
BshVI ATCGAT 3 cut(s) 60, 299, 576
BsiHKCI CYCGRG 1 cut(s) 117
BsiSI CCGG 2 cut(s) 627, 888
BslFI GGGAC 2 cut(s) 473, 898
BslI CCNNNNNNNGG 3 cut(s) 627, 628, 943
BsmAI GTCTC 3 cut(s) 312, 391, 421
BsmBI CGTCTC 1 cut(s) 421
BsmFI GGGAC 2 cut(s) 473, 898
BsnI GGCC 2 cut(s) 122, 936
BsoBI CYCGRG 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 930
Bsp1286I GDGCHC 1 cut(s) 634
Bsp143I GATC 6 cut(s) 57, 142, 157, 573, 610, 859
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 3 cut(s) 179, 438, 594
BspANI GGCC 2 cut(s) 122, 936
BspCNI CTCAG 3 cut(s) 178, 379, 559
BspDI ATCGAT 3 cut(s) 60, 299, 576
BspFNI CGCG 2 cut(s) 592, 594
BspOI GCTAGC 2 cut(s) 249, 345
BspPI GGATC 1 cut(s) 150
BspT104I TTCGAA 1 cut(s) 930
BsrDI GCAATG 1 cut(s) 997
BsrFI RCCGGY 1 cut(s) 887
BsrI ACTGG 1 cut(s) 937
BssAI RCCGGY 1 cut(s) 887
BssECI CCNNGG 3 cut(s) 116, 138, 627
BssHII GCGCGC 1 cut(s) 590
BssMI GATC 6 cut(s) 57, 142, 157, 573, 610, 859
BssNI GRCGYC 2 cut(s) 786, 911
Bst4CI ACNGT 4 cut(s) 24, 402, 415, 539
BstACI GRCGYC 2 cut(s) 786, 911
BstBI TTCGAA 1 cut(s) 930
BstC8I GCNNGC 6 cut(s) 179, 183, 247, 260, 343, 592
BstDEI CTNAG 5 cut(s) 78, 186, 353, 387, 546
BstEII GGTNACC 1 cut(s) 616
BstFNI CGCG 2 cut(s) 592, 594
BstHHI GCGC 3 cut(s) 501, 592, 594
BstKTI GATC 6 cut(s) 60, 145, 160, 576, 613, 862
BstMAI GTCTC 3 cut(s) 312, 391, 421
BstMBI GATC 6 cut(s) 57, 142, 157, 573, 610, 859
BstMWI GCNNNNNNNGC 3 cut(s) 134, 255, 908
BstNSI RCATGY 2 cut(s) 177, 607
BstPI GGTNACC 1 cut(s) 616
BstSCI CCNGG 1 cut(s) 626
BstSLI GKGCMC 1 cut(s) 634
BstUI CGCG 2 cut(s) 592, 594
BstV1I GCAGC 6 cut(s) 107, 229, 275, 427, 852, 889
BstX2I RGATCY 1 cut(s) 142
BstYI RGATCY 1 cut(s) 142
Bsu15I ATCGAT 3 cut(s) 60, 299, 576
Bsu36I CCTNAGG 1 cut(s) 546
BsuI GTATCC 1 cut(s) 545
BsuRI GGCC 2 cut(s) 122, 936
BsuTUI ATCGAT 3 cut(s) 60, 299, 576
BtsIMutI CAGTG 3 cut(s) 20, 407, 544
Cac8I GCNNGC 6 cut(s) 179, 183, 247, 260, 343, 592
CfoI GCGC 3 cut(s) 501, 592, 594
Cfr10I RCCGGY 1 cut(s) 887
Cfr13I GGNCC 3 cut(s) 120, 473, 890
ClaI ATCGAT 3 cut(s) 60, 299, 576
CseI GACGC 3 cut(s) 302, 794, 900
Csp6I GTAC 2 cut(s) 727, 817
CspAI ACCGGT 1 cut(s) 887
CviAII CATG 4 cut(s) 174, 420, 604, 724
CviQI GTAC 2 cut(s) 727, 817
DdeI CTNAG 5 cut(s) 78, 186, 353, 387, 546
DpnI GATC 6 cut(s) 59, 144, 159, 575, 612, 861
DpnII GATC 6 cut(s) 57, 142, 157, 573, 610, 859
Eco32I GATATC 1 cut(s) 379
Eco47I GGWCC 2 cut(s) 473, 890
Eco81I CCTNAGG 1 cut(s) 546
Eco88I CYCGRG 1 cut(s) 117
Eco91I GGTNACC 1 cut(s) 616
EcoO65I GGTNACC 1 cut(s) 616
EcoRV GATATC 1 cut(s) 379
Esp3I CGTCTC 1 cut(s) 421
FaeI CATG 4 cut(s) 177, 423, 607, 727
FaiI YATR 8 cut(s) 89, 165, 175, 421, 464, 605, 725, 791
FaqI GGGAC 2 cut(s) 473, 898
FatI CATG 4 cut(s) 173, 419, 603, 723
FauI CCCGC 1 cut(s) 186
FbaI TGATCA 2 cut(s) 157, 859
Fnu4HI GCNGC 7 cut(s) 96, 243, 289, 441, 595, 866, 903
Fsp4HI GCNGC 7 cut(s) 96, 243, 289, 441, 595, 866, 903
FspBI CTAG 3 cut(s) 246, 342, 447
GlaI GCGC 3 cut(s) 500, 591, 593
GluI GCNGC 7 cut(s) 96, 243, 289, 441, 595, 866, 903
GsaI CCCAGC 1 cut(s) 919
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 2 cut(s) 122, 936
HapII CCGG 2 cut(s) 627, 888
HgaI GACGC 3 cut(s) 302, 794, 900
HhaI GCGC 3 cut(s) 501, 592, 594
Hin1I GRCGYC 2 cut(s) 786, 911
Hin1II CATG 4 cut(s) 177, 423, 607, 727
Hin6I GCGC 3 cut(s) 499, 590, 592
HinP1I GCGC 3 cut(s) 499, 590, 592
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HindIII AAGCTT 1 cut(s) 959
HinfI GANTC 4 cut(s) 192, 220, 301, 826
HpaII CCGG 2 cut(s) 627, 888
HphI GGTGA 4 cut(s) 85, 427, 610, 967
Hpy166II GTNNAC 2 cut(s) 855, 1012
Hpy188I TCNGA 3 cut(s) 212, 877, 1007
Hpy188III TCNNGA 4 cut(s) 55, 317, 742, 782
Hpy8I GTNNAC 2 cut(s) 855, 1012
Hpy99I CGWCG 4 cut(s) 296, 377, 788, 925
HpyAV CCTTC 4 cut(s) 673, 720, 926, 989
HpyCH4III ACNGT 4 cut(s) 24, 402, 415, 539
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 5 cut(s) 262, 443, 756, 905, 1017
HpyF10VI GCNNNNNNNGC 3 cut(s) 134, 255, 908
HpyF3I CTNAG 5 cut(s) 78, 186, 353, 387, 546
HpySE526I ACGT 1 cut(s) 105
Hsp92I GRCGYC 2 cut(s) 786, 911
Hsp92II CATG 4 cut(s) 177, 423, 607, 727
HspAI GCGC 3 cut(s) 499, 590, 592
Ksp22I TGATCA 2 cut(s) 157, 859
Kzo9I GATC 6 cut(s) 57, 142, 157, 573, 610, 859
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 9 cut(s) 331, 453, 533, 599, 640, 658, 901, 929, 950
Lsp1109I GCAGC 6 cut(s) 107, 229, 275, 427, 852, 889
LweI GCATC 3 cut(s) 258, 305, 442
MaeI CTAG 3 cut(s) 246, 342, 447
MaeII ACGT 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 73, 81, 616
MalI GATC 6 cut(s) 59, 144, 159, 575, 612, 861
MboI GATC 6 cut(s) 57, 142, 157, 573, 610, 859
MboII GAAGA 4 cut(s) 137, 757, 760, 821
MfeI CAATTG 1 cut(s) 569
MflI RGATCY 1 cut(s) 142
MhlI GDGCHC 1 cut(s) 634
MluCI AATT 6 cut(s) 512, 569, 682, 736, 871, 1018
MlyI GAGTC 1 cut(s) 214
MmeI TCCRAC 2 cut(s) 24, 190
MseI TTAA 4 cut(s) 168, 309, 717, 731
MspA1I CMGCKG 2 cut(s) 440, 865
MspI CCGG 2 cut(s) 627, 888
MspR9I CCNGG 1 cut(s) 628
MunI CAATTG 1 cut(s) 569
MvnI CGCG 2 cut(s) 592, 594
MwoI GCNNNNNNNGC 3 cut(s) 134, 255, 908
NciI CCSGG 1 cut(s) 628
NdeII GATC 6 cut(s) 57, 142, 157, 573, 610, 859
NheI GCTAGC 2 cut(s) 245, 341
NlaIII CATG 4 cut(s) 177, 423, 607, 727
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 2 cut(s) 73, 616
NspI RCATGY 2 cut(s) 177, 607
NspV TTCGAA 1 cut(s) 930
PauI GCGCGC 1 cut(s) 590
PfeI GAWTC 3 cut(s) 192, 301, 826
PflFI GACNNNGTC 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 887
PkrI GCNGC 7 cut(s) 97, 244, 290, 442, 596, 867, 904
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
PspEI GGTNACC 1 cut(s) 616
PspFI CCCAGC 1 cut(s) 915
PspPI GGNCC 3 cut(s) 120, 473, 890
PsuI RGATCY 1 cut(s) 142
PsyI GACNNNGTC 1 cut(s) 402
PteI GCGCGC 1 cut(s) 590
PvuII CAGCTG 1 cut(s) 865
RsaI GTAC 2 cut(s) 728, 818
RsaNI GTAC 2 cut(s) 727, 817
SaqAI TTAA 4 cut(s) 168, 309, 717, 731
SatI GCNGC 7 cut(s) 96, 243, 289, 441, 595, 866, 903
Sau3AI GATC 6 cut(s) 57, 142, 157, 573, 610, 859
Sau96I GGNCC 3 cut(s) 120, 473, 890
SchI GAGTC 1 cut(s) 214
ScrFI CCNGG 1 cut(s) 628
SduI GDGCHC 1 cut(s) 634
SfaNI GCATC 3 cut(s) 258, 305, 442
SfuI TTCGAA 1 cut(s) 930
SinI GGWCC 2 cut(s) 473, 890
SmlI CTYRAG 2 cut(s) 392, 524
SmoI CTYRAG 2 cut(s) 392, 524
Sse9I AATT 6 cut(s) 512, 569, 682, 736, 871, 1018
SsiI CCGC 3 cut(s) 179, 438, 594
SspMI CTAG 3 cut(s) 246, 342, 447
StyD4I CCNGG 1 cut(s) 626
TaaI ACNGT 4 cut(s) 24, 402, 415, 539
TaiI ACGT 1 cut(s) 108
TaqI TCGA 9 cut(s) 60, 299, 316, 372, 576, 783, 807, 824, 930
TasI AATT 6 cut(s) 512, 569, 682, 736, 871, 1018
TatI WGTACW 2 cut(s) 726, 816
TauI GCSGC 1 cut(s) 597
TfiI GAWTC 3 cut(s) 192, 301, 826
Tru1I TTAA 4 cut(s) 168, 309, 717, 731
Tru9I TTAA 4 cut(s) 168, 309, 717, 731
TscAI CASTG 3 cut(s) 27, 407, 544
TseFI GTSAC 2 cut(s) 73, 616
TseI GCWGC 6 cut(s) 95, 242, 288, 440, 865, 902
Tsp45I GTSAC 2 cut(s) 73, 616
TspDTI ATGAA 2 cut(s) 17, 1008
TspRI CASTG 3 cut(s) 27, 407, 544
Tth111I GACNNNGTC 1 cut(s) 402
VpaK11BI GGWCC 2 cut(s) 473, 890
XapI RAATTY 2 cut(s) 512, 682
XceI RCATGY 2 cut(s) 177, 607
XspI CTAG 3 cut(s) 246, 342, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.