RchiOBHm_Chr5g0023121

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004070
Physical Location & Seq
Forward (+)
17092653 .. 17093980
1328 bp
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UTR
Exon/CDS
Intron
PRQ30301

Sequence Viewer

Length: 1014 bp
ATGAAGATGGGAATTTCTACATTATTTGCTCTCCTTGTTCTCACACTGGAGTTGTTCATGAGCATTAATGCATCCGCTGCTAAACCAGTCAAAGCAACTGCACAGGTGCCTGCTGAGGCTCTTTTGTCTTTCAGTCACTACATGAAAACTTGTCCACAAGCAGAGGGCATCATTCAGCAAAAAGTCGTAGACTGGCTCGAGAAGGATTTCACTTTGGCTGCTAGCATCATCCGCTTACACTTCCATGATTGTGTTGTGAGGGGTTGCGATGCATCAATTTTGCTTAACCATCGGGGGAGTGAGAGGAGAGCTTTTGCAAGCTATACACTCAGAGGTTTCCAAGTAATCGATGACATCAAGGCGGAGCTTGAGAGGCAGTGCCCTAAAACTGTGTCTTGCGCGGACATTCTCACTGCTGCAACGAGAGATGCCACCATCATGGCCGGAGGTCCGTTTTGGGAAGTCCCATTCGGGCGTAGAGATGGGAAGATTTCGATTGCCAGAGAAGCGGATGAGAAGGTCCCTCAGGGCCATGAGAATATCACTGCATTGATCCAACTATTTCGCGAAAAAGGCTTGAATATGCTTGATTTGGTTGCTCTGTCAGGAGCACACACCATTGGTAGAAGTACTTGCCTTGCCTTCAAGCAAAGGCTTGCAAACTTCAATGGAACCAGAAAGCCTGACCCTTCACTAAATTCTATGTACTTGAACAACTTCTTGAAGAAGAAATGTCAAAAAGACTCGGACTTTGTTTTTCTTGATGCCATAACTCCGAAGACATTTGACACGCAATACTACACGAATCTTCACAAGAAGTTGGGGTTGTTATCCACTGATCAGTTGCTCAAGTCAGATATGAGAACCGGTCCGTTTGTTGATGCATTAGGTTCCCAGCCTTCTCTTTTCGAGAGCCAGTTTGCAGCATCCATGGTGAAGCTTGGAAATGTACAAGTTCTTACAAGACCCAATGAAGGCGAGATCCGAGTGAACTGCAATTTTGTCAATGCTTAA

Protein Analysis

337

Amino Acids

37.38

Weight (kDa)

8.84

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 59 - 296 2.6e-78 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 189
AccII CGCG 2 cut(s) 401, 567
AciI CCGC 5 cut(s) 75, 232, 362, 401, 509
AclWI GGATC 2 cut(s) 547, 976
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 2 cut(s) 12, 697
AfaI GTAC 3 cut(s) 631, 707, 951
AfiI CCNNNNNNNGG 1 cut(s) 974
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 5 cut(s) 580, 646, 667, 712, 724
AluBI AGCT 4 cut(s) 311, 321, 367, 940
AluI AGCT 4 cut(s) 311, 321, 367, 940
Alw21I GWGCWC 1 cut(s) 613
AlwI GGATC 2 cut(s) 547, 976
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 441, 529
ApeKI GCWGC 4 cut(s) 77, 218, 416, 923
ApoI RAATTY 2 cut(s) 12, 697
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AseI ATTAAT 1 cut(s) 66
AsiGI ACCGGT 1 cut(s) 866
Asp700I GAANNNNTTC 2 cut(s) 206, 716
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 4 cut(s) 449, 520, 529, 869
AsuHPI GGTGA 1 cut(s) 946
AsuNHI GCTAGC 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 3 cut(s) 449, 520, 869
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 1 cut(s) 383
BanI GGYRCC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 785
Bbv12I GWGCWC 1 cut(s) 613
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 4 cut(s) 64, 205, 403, 935
BccI CCATC 3 cut(s) 297, 443, 476
BcgI CGANNNNNNTGC 2 cut(s) 975, 1009
BclI TGATCA 1 cut(s) 838
BfaI CTAG 1 cut(s) 222
BisI GCNGC 4 cut(s) 78, 219, 417, 924
BlsI GCNGC 4 cut(s) 79, 220, 418, 925
BmcAI AGTACT 1 cut(s) 631
Bme18I GGWCC 3 cut(s) 449, 520, 869
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 4 cut(s) 449, 520, 529, 869
BmiI GGNNCC 4 cut(s) 108, 522, 673, 892
BmsI GCATC 9 cut(s) 80, 177, 234, 259, 281, 418, 754, 871, 935
BmtI GCTAGC 1 cut(s) 225
BpiI GAAGAC 1 cut(s) 785
BpmI CTGGAG 1 cut(s) 68
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 389, 833
Bsa29I ATCGAT 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 930
BsaWI WCCGGW 1 cut(s) 866
Bsc4I CCNNNNNNNGG 1 cut(s) 974
Bse118I RCCGGY 1 cut(s) 866
Bse1I ACTGG 4 cut(s) 51, 86, 197, 916
Bse21I CCTNAGG 1 cut(s) 525
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 930
BseGI GGATG 4 cut(s) 71, 228, 517, 926
BseLI CCNNNNNNNGG 1 cut(s) 974
BseMII CTCAG 3 cut(s) 105, 343, 539
BseNI ACTGG 4 cut(s) 51, 86, 197, 916
BseRI GAGGAG 1 cut(s) 319
BseSI GKGCMC 1 cut(s) 383
BseXI GCAGC 4 cut(s) 64, 205, 403, 935
BseYI CCCAGC 1 cut(s) 894
BsgI GTGCAG 1 cut(s) 84
Bsh1236I CGCG 2 cut(s) 401, 567
BshFI GGCC 2 cut(s) 443, 531
BshNI GGYRCC 1 cut(s) 106
BshTI ACCGGT 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 348
BsiHKAI GWGCWC 1 cut(s) 613
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 2 cut(s) 444, 867
BslFI GGGAC 2 cut(s) 449, 506
BslI CCNNNNNNNGG 1 cut(s) 974
BsmFI GGGAC 2 cut(s) 449, 506
BsnI GGCC 2 cut(s) 443, 531
BsoBI CYCGRG 1 cut(s) 197
Bsp1286I GDGCHC 2 cut(s) 383, 613
Bsp1407I TGTACA 1 cut(s) 949
Bsp143I GATC 3 cut(s) 552, 838, 981
Bsp19I CCATGG 1 cut(s) 930
Bsp68I TCGCGA 1 cut(s) 567
BspACI CCGC 5 cut(s) 75, 232, 362, 401, 509
BspANI GGCC 2 cut(s) 443, 531
BspCNI CTCAG 3 cut(s) 106, 342, 538
BspDI ATCGAT 1 cut(s) 348
BspFNI CGCG 2 cut(s) 401, 567
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 4 cut(s) 108, 522, 673, 892
BspOI GCTAGC 1 cut(s) 225
BspPI GGATC 2 cut(s) 547, 976
BspT107I GGYRCC 1 cut(s) 106
BsrFI RCCGGY 1 cut(s) 866
BsrGI TGTACA 1 cut(s) 949
BsrI ACTGG 4 cut(s) 51, 86, 197, 916
BssAI RCCGGY 1 cut(s) 866
BssECI CCNNGG 1 cut(s) 930
BssMI GATC 3 cut(s) 552, 838, 981
BssT1I CCWWGG 1 cut(s) 930
Bst4CI ACNGT 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 949
BstC8I GCNNGC 4 cut(s) 111, 223, 319, 657
BstDEI CTNAG 3 cut(s) 114, 329, 525
BstDSI CCRYGG 1 cut(s) 930
BstF5I GGATG 4 cut(s) 71, 228, 517, 926
BstFNI CGCG 2 cut(s) 401, 567
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 3 cut(s) 555, 841, 984
BstMBI GATC 3 cut(s) 552, 838, 981
BstMWI GCNNNNNNNGC 5 cut(s) 77, 231, 373, 506, 573
BstSLI GKGCMC 1 cut(s) 383
BstUI CGCG 2 cut(s) 401, 567
BstV1I GCAGC 4 cut(s) 64, 205, 403, 935
BstV2I GAAGAC 1 cut(s) 785
BstX2I RGATCY 1 cut(s) 981
BstXI CCANNNNNNTGG 1 cut(s) 439
BstYI RGATCY 1 cut(s) 981
Bsu15I ATCGAT 1 cut(s) 348
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 2 cut(s) 443, 531
BsuTUI ATCGAT 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 4 cut(s) 71, 228, 517, 926
BtsI GCAGTG 3 cut(s) 383, 411, 543
BtsIMutI CAGTG 5 cut(s) 44, 383, 411, 543, 834
BtuMI TCGCGA 1 cut(s) 567
Cac8I GCNNGC 4 cut(s) 111, 223, 319, 657
CciI TCATGA 1 cut(s) 57
CfoI GCGC 1 cut(s) 401
Cfr10I RCCGGY 1 cut(s) 866
Cfr13I GGNCC 4 cut(s) 449, 520, 529, 869
ClaI ATCGAT 1 cut(s) 348
CpoI CGGWCCG 1 cut(s) 869
Csp6I GTAC 3 cut(s) 630, 706, 950
CspAI ACCGGT 1 cut(s) 866
CspI CGGWCCG 1 cut(s) 869
CviAII CATG 6 cut(s) 58, 142, 245, 439, 533, 931
CviQI GTAC 3 cut(s) 630, 706, 950
DdeI CTNAG 3 cut(s) 114, 329, 525
DpnI GATC 3 cut(s) 554, 840, 983
DpnII GATC 3 cut(s) 552, 838, 981
EaeI YGGCCR 1 cut(s) 441
EciI GGCGGA 1 cut(s) 377
Eco130I CCWWGG 1 cut(s) 930
Eco47I GGWCC 3 cut(s) 449, 520, 869
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 930
EcoT22I ATGCAT 3 cut(s) 73, 274, 886
ErhI CCWWGG 1 cut(s) 930
FaeI CATG 6 cut(s) 61, 145, 248, 442, 536, 934
FaqI GGGAC 2 cut(s) 449, 506
FatI CATG 6 cut(s) 57, 141, 244, 438, 532, 930
FbaI TGATCA 1 cut(s) 838
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 4 cut(s) 78, 219, 417, 924
FokI GGATG 4 cut(s) 58, 215, 524, 913
Fsp4HI GCNGC 4 cut(s) 78, 219, 417, 924
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 400
GluI GCNGC 4 cut(s) 78, 219, 417, 924
GsaI CCCAGC 1 cut(s) 898
GsuI CTGGAG 1 cut(s) 68
HaeIII GGCC 2 cut(s) 443, 531
HapII CCGG 2 cut(s) 444, 867
HhaI GCGC 1 cut(s) 401
Hin1II CATG 6 cut(s) 61, 145, 248, 442, 536, 934
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HindIII AAGCTT 1 cut(s) 938
HinfI GANTC 2 cut(s) 743, 805
HpaII CCGG 2 cut(s) 444, 867
HphI GGTGA 1 cut(s) 946
Hpy166II GTNNAC 3 cut(s) 155, 190, 991
Hpy188I TCNGA 5 cut(s) 332, 748, 777, 856, 986
Hpy188III TCNNGA 7 cut(s) 58, 199, 566, 606, 721, 761, 910
Hpy8I GTNNAC 3 cut(s) 155, 190, 991
HpyAV CCTTC 6 cut(s) 196, 511, 652, 699, 909, 968
HpyCH4III ACNGT 1 cut(s) 391
HpyF10VI GCNNNNNNNGC 5 cut(s) 77, 231, 373, 506, 573
HpyF3I CTNAG 3 cut(s) 114, 329, 525
Hsp92II CATG 6 cut(s) 61, 145, 248, 442, 536, 934
HspAI GCGC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 838
Kzo9I GATC 3 cut(s) 552, 838, 981
LmnI GCTCC 2 cut(s) 364, 608
Lsp1109I GCAGC 4 cut(s) 64, 205, 403, 935
LweI GCATC 9 cut(s) 80, 177, 234, 259, 281, 418, 754, 871, 935
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 134
MalI GATC 3 cut(s) 554, 840, 983
MboI GATC 3 cut(s) 552, 838, 981
MboII GAAGA 6 cut(s) 16, 499, 736, 739, 790, 800
MflI RGATCY 1 cut(s) 981
MhlI GDGCHC 2 cut(s) 383, 613
MluCI AATT 4 cut(s) 12, 276, 697, 997
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 1 cut(s) 580
MnlI CCTC 8 cut(s) 109, 157, 252, 297, 326, 366, 440, 534
Mph1103I ATGCAT 3 cut(s) 73, 274, 886
MroXI GAANNNNTTC 2 cut(s) 206, 716
MseI TTAA 3 cut(s) 66, 285, 1012
MslI CAYNNNNRTG 3 cut(s) 243, 249, 437
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 2 cut(s) 444, 867
MvnI CGCG 2 cut(s) 401, 567
MwoI GCNNNNNNNGC 5 cut(s) 77, 231, 373, 506, 573
NcoI CCATGG 1 cut(s) 930
NdeII GATC 3 cut(s) 552, 838, 981
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 6 cut(s) 61, 145, 248, 442, 536, 934
NlaIV GGNNCC 4 cut(s) 108, 522, 673, 892
NmuCI GTSAC 1 cut(s) 134
NruI TCGCGA 1 cut(s) 567
NsiI ATGCAT 3 cut(s) 73, 274, 886
PaeR7I CTCGAG 1 cut(s) 197
PagI TCATGA 1 cut(s) 57
PdmI GAANNNNTTC 2 cut(s) 206, 716
PfeI GAWTC 1 cut(s) 805
PinAI ACCGGT 1 cut(s) 866
PkrI GCNGC 4 cut(s) 79, 220, 418, 925
PleI GAGTC 1 cut(s) 737
PpsI GAGTC 1 cut(s) 737
PpuMI RGGWCCY 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 520
PspFI CCCAGC 1 cut(s) 894
PspN4I GGNNCC 4 cut(s) 108, 522, 673, 892
PspPI GGNCC 4 cut(s) 449, 520, 529, 869
PspPPI RGGWCCY 1 cut(s) 520
PsuI RGATCY 1 cut(s) 981
RruI TCGCGA 1 cut(s) 567
RsaI GTAC 3 cut(s) 631, 707, 951
RsaNI GTAC 3 cut(s) 630, 706, 950
RseI CAYNNNNRTG 3 cut(s) 243, 249, 437
Rsr2I CGGWCCG 1 cut(s) 869
RsrII CGGWCCG 1 cut(s) 869
SaqAI TTAA 3 cut(s) 66, 285, 1012
SatI GCNGC 4 cut(s) 78, 219, 417, 924
Sau3AI GATC 3 cut(s) 552, 838, 981
Sau96I GGNCC 4 cut(s) 449, 520, 529, 869
ScaI AGTACT 1 cut(s) 631
SchI GAGTC 1 cut(s) 737
SduI GDGCHC 2 cut(s) 383, 613
SetI ASST 9 cut(s) 108, 313, 323, 337, 369, 451, 522, 892, 942
SfaNI GCATC 9 cut(s) 80, 177, 234, 259, 281, 418, 754, 871, 935
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 3 cut(s) 449, 520, 869
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 3 cut(s) 243, 249, 437
SmlI CTYRAG 3 cut(s) 197, 368, 848
SmoI CTYRAG 3 cut(s) 197, 368, 848
Sse9I AATT 4 cut(s) 12, 276, 697, 997
SsiI CCGC 5 cut(s) 75, 232, 362, 401, 509
SspMI CTAG 1 cut(s) 222
StyI CCWWGG 1 cut(s) 930
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 4 cut(s) 198, 348, 494, 909
TasI AATT 4 cut(s) 12, 276, 697, 997
TatI WGTACW 3 cut(s) 629, 705, 949
TfiI GAWTC 1 cut(s) 805
Tru1I TTAA 3 cut(s) 66, 285, 1012
Tru9I TTAA 3 cut(s) 66, 285, 1012
TscAI CASTG 5 cut(s) 51, 383, 418, 550, 841
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 4 cut(s) 77, 218, 416, 923
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 4 cut(s) 17, 46, 158, 987
TspGWI ACGGA 2 cut(s) 441, 861
TspRI CASTG 5 cut(s) 51, 383, 418, 550, 841
VpaK11BI GGWCC 3 cut(s) 449, 520, 869
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 2 cut(s) 12, 697
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 2 cut(s) 206, 716
XspI CTAG 1 cut(s) 222
ZrmI AGTACT 1 cut(s) 631
Zsp2I ATGCAT 3 cut(s) 73, 274, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.