Rroxscaffold_1G00056230

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004070
Physical Location & Seq
Reverse (-)
77996701 .. 77997546
846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00056230.1

Sequence Viewer

Length: 537 bp
ATGAAGATGGGAATTTCTACATTATTTGCTCTCCTTGTTCTCACATTGGAGTTGTTCATGACTATTAATGCATCCGCTGCTAAACCAGTCAAAGCAACTGCACAGGTGCCTGCTGAGGCTCTTTTGTCTTTCAGTCACTACATGAAAACTTGTCCACAAGCAGAGGGCATCATTCAGCAAAAAGTCGTAGACTGGCTCGAGAAGGATTTCACTTTGGCTGCTAGCATTATCCGCTTACACTTCCATGATTGCGTTGTGAGGGGTTGCGATGCATCAATTTTGCTTAACCATCGGGGGAGTGAGAGGAGAGCTTTTGCAAGCTATACACTCAGAGGTTTCCAAGTAATCGATGACATCAAGGCGGAGCTTGAGAGGCAGTGTCCTAAAACTGTGTCTTGCGCAGACATTCTCACTGCTGCAACAAGAGATGCCACCATCATGGCCGGAGGTCCGTTTTGGGAAGTCCCATTCGGGCGTAGAGATGGGAAGATTTCGATTGCCAGAGAAGCGGATGAGAAGGTCCCCTCAGGGCCATGA

Protein Analysis

178

Amino Acids

19.55

Weight (kDa)

7.66

Isoelectric Point (pI)

36.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 59 - 176 3.9e-41 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 189
AciI CCGC 4 cut(s) 75, 232, 362, 509
AcoI YGGCCR 1 cut(s) 441
AcsI RAATTY 1 cut(s) 12
AluBI AGCT 3 cut(s) 311, 321, 367
AluI AGCT 3 cut(s) 311, 321, 367
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 2 cut(s) 441, 530
ApeKI GCWGC 3 cut(s) 77, 218, 416
ApoI RAATTY 1 cut(s) 12
AseI ATTAAT 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 401
AspS9I GGNCC 3 cut(s) 449, 520, 530
AsuNHI GCTAGC 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 197
AvaII GGWCC 2 cut(s) 449, 520
AxyI CCTNAGG 1 cut(s) 526
BanI GGYRCC 1 cut(s) 106
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 3 cut(s) 64, 205, 403
BccI CCATC 3 cut(s) 297, 443, 476
BfaI CTAG 1 cut(s) 222
BisI GCNGC 3 cut(s) 78, 219, 417
BlsI GCNGC 3 cut(s) 79, 220, 418
Bme18I GGWCC 2 cut(s) 449, 520
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 3 cut(s) 449, 520, 530
BmiI GGNNCC 2 cut(s) 108, 522
BmsI GCATC 5 cut(s) 80, 177, 259, 281, 418
BmtI GCTAGC 1 cut(s) 225
Bpu10I CCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 348
Bse1I ACTGG 2 cut(s) 86, 197
Bse21I CCTNAGG 1 cut(s) 526
BseCI ATCGAT 1 cut(s) 348
BseGI GGATG 2 cut(s) 71, 517
BseMII CTCAG 2 cut(s) 105, 343
BseNI ACTGG 2 cut(s) 86, 197
BseRI GAGGAG 1 cut(s) 319
BseXI GCAGC 3 cut(s) 64, 205, 403
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 2 cut(s) 443, 532
BshNI GGYRCC 1 cut(s) 106
BshVI ATCGAT 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 444
BslFI GGGAC 2 cut(s) 449, 506
BsmFI GGGAC 2 cut(s) 449, 506
BsnI GGCC 2 cut(s) 443, 532
BsoBI CYCGRG 1 cut(s) 197
BspACI CCGC 4 cut(s) 75, 232, 362, 509
BspANI GGCC 2 cut(s) 443, 532
BspCNI CTCAG 2 cut(s) 106, 342
BspDI ATCGAT 1 cut(s) 348
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 108, 522
BspOI GCTAGC 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 106
BsrI ACTGG 2 cut(s) 86, 197
Bst4CI ACNGT 1 cut(s) 391
BstAPI GCANNNNNTGC 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 111, 223, 319
BstDEI CTNAG 3 cut(s) 114, 329, 526
BstF5I GGATG 2 cut(s) 71, 517
BstHHI GCGC 1 cut(s) 401
BstMWI GCNNNNNNNGC 4 cut(s) 77, 231, 373, 506
BstV1I GCAGC 3 cut(s) 64, 205, 403
BstXI CCANNNNNNTGG 1 cut(s) 439
Bsu15I ATCGAT 1 cut(s) 348
Bsu36I CCTNAGG 1 cut(s) 526
BsuRI GGCC 2 cut(s) 443, 532
BsuTUI ATCGAT 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 2 cut(s) 71, 517
BtsI GCAGTG 2 cut(s) 383, 411
BtsIMutI CAGTG 2 cut(s) 383, 411
Cac8I GCNNGC 3 cut(s) 111, 223, 319
CciI TCATGA 1 cut(s) 57
CfoI GCGC 1 cut(s) 401
Cfr13I GGNCC 3 cut(s) 449, 520, 530
ClaI ATCGAT 1 cut(s) 348
CviAII CATG 5 cut(s) 58, 142, 245, 439, 534
CviJI RGCY 8 cut(s) 119, 196, 218, 311, 321, 367, 443, 532
CviKI_1 RGCY 8 cut(s) 119, 196, 218, 311, 321, 367, 443, 532
DdeI CTNAG 3 cut(s) 114, 329, 526
EaeI YGGCCR 1 cut(s) 441
EciI GGCGGA 1 cut(s) 377
Eco47I GGWCC 2 cut(s) 449, 520
Eco81I CCTNAGG 1 cut(s) 526
Eco88I CYCGRG 1 cut(s) 197
EcoO109I RGGNCCY 1 cut(s) 520
EcoT22I ATGCAT 2 cut(s) 73, 274
FaeI CATG 5 cut(s) 61, 145, 248, 442, 537
FaiI YATR 6 cut(s) 59, 143, 246, 324, 440, 535
FaqI GGGAC 2 cut(s) 449, 506
FatI CATG 5 cut(s) 57, 141, 244, 438, 533
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 78, 219, 417
FokI GGATG 2 cut(s) 58, 524
Fsp4HI GCNGC 3 cut(s) 78, 219, 417
FspBI CTAG 1 cut(s) 222
FspI TGCGCA 1 cut(s) 400
GlaI GCGC 1 cut(s) 400
GluI GCNGC 3 cut(s) 78, 219, 417
HaeIII GGCC 2 cut(s) 443, 532
HapII CCGG 1 cut(s) 444
HhaI GCGC 1 cut(s) 401
Hin1II CATG 5 cut(s) 61, 145, 248, 442, 537
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HpaII CCGG 1 cut(s) 444
Hpy166II GTNNAC 2 cut(s) 155, 190
Hpy188I TCNGA 1 cut(s) 332
Hpy188III TCNNGA 2 cut(s) 58, 199
Hpy8I GTNNAC 2 cut(s) 155, 190
HpyAV CCTTC 2 cut(s) 196, 511
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4V TGCA 5 cut(s) 71, 101, 272, 317, 419
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 231, 373, 506
HpyF3I CTNAG 3 cut(s) 114, 329, 526
Hsp92II CATG 5 cut(s) 61, 145, 248, 442, 537
HspAI GCGC 1 cut(s) 399
LmnI GCTCC 1 cut(s) 364
LpnPI CCDG 7 cut(s) 89, 99, 123, 178, 457, 513, 514
Lsp1109I GCAGC 3 cut(s) 64, 205, 403
LweI GCATC 5 cut(s) 80, 177, 259, 281, 418
MaeI CTAG 1 cut(s) 222
MaeIII GTNAC 1 cut(s) 134
MboII GAAGA 2 cut(s) 16, 499
MluCI AATT 2 cut(s) 12, 276
MnlI CCTC 8 cut(s) 109, 157, 252, 297, 326, 366, 440, 535
Mph1103I ATGCAT 2 cut(s) 73, 274
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 2 cut(s) 66, 285
MslI CAYNNNNRTG 2 cut(s) 243, 437
MspA1I CMGCKG 1 cut(s) 77
MspI CCGG 1 cut(s) 444
MwoI GCNNNNNNNGC 4 cut(s) 77, 231, 373, 506
NheI GCTAGC 1 cut(s) 221
NlaIII CATG 5 cut(s) 61, 145, 248, 442, 537
NlaIV GGNNCC 2 cut(s) 108, 522
NmuCI GTSAC 1 cut(s) 134
NsbI TGCGCA 1 cut(s) 400
NsiI ATGCAT 2 cut(s) 73, 274
PaeR7I CTCGAG 1 cut(s) 197
PagI TCATGA 1 cut(s) 57
PdmI GAANNNNTTC 1 cut(s) 206
PkrI GCNGC 3 cut(s) 79, 220, 418
PpuMI RGGWCCY 1 cut(s) 520
PshBI ATTAAT 1 cut(s) 66
Psp5II RGGWCCY 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 108, 522
PspPI GGNCC 3 cut(s) 449, 520, 530
PspPPI RGGWCCY 1 cut(s) 520
RseI CAYNNNNRTG 2 cut(s) 243, 437
SaqAI TTAA 2 cut(s) 66, 285
SatI GCNGC 3 cut(s) 78, 219, 417
Sau96I GGNCC 3 cut(s) 449, 520, 530
SetI ASST 7 cut(s) 108, 313, 323, 337, 369, 451, 522
SfaNI GCATC 5 cut(s) 80, 177, 259, 281, 418
Sfr274I CTCGAG 1 cut(s) 197
SinI GGWCC 2 cut(s) 449, 520
SlaI CTCGAG 1 cut(s) 197
SmiMI CAYNNNNRTG 2 cut(s) 243, 437
SmlI CTYRAG 2 cut(s) 197, 368
SmoI CTYRAG 2 cut(s) 197, 368
Sse9I AATT 2 cut(s) 12, 276
SsiI CCGC 4 cut(s) 75, 232, 362, 509
SspMI CTAG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 198, 348, 494
TasI AATT 2 cut(s) 12, 276
Tru1I TTAA 2 cut(s) 66, 285
Tru9I TTAA 2 cut(s) 66, 285
TscAI CASTG 2 cut(s) 383, 418
TseFI GTSAC 1 cut(s) 134
TseI GCWGC 3 cut(s) 77, 218, 416
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 3 cut(s) 17, 46, 158
TspGWI ACGGA 1 cut(s) 441
TspRI CASTG 2 cut(s) 383, 418
VpaK11BI GGWCC 2 cut(s) 449, 520
VspI ATTAAT 1 cut(s) 66
XapI RAATTY 1 cut(s) 12
XhoI CTCGAG 1 cut(s) 197
XmiI GTMKAC 1 cut(s) 189
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 1 cut(s) 222
Zsp2I ATGCAT 2 cut(s) 73, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.