pycom05g21840

Protein of unknown function (DUF1639)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0006362
Physical Location & Seq
Reverse (-)
24416027 .. 24418582
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 705 bp
ATGCCGCCGCCTCCTCCTCCGCCGCCCCTTCCGTCTTCTTCCTCCGCTACGTCGTCGTTTTCCAACGGCAGCAGACTGAGAAAGGAAGGCTCCGGGTCGCTTCCTAGCAGGAATTTGGTGGATCGATCTGCTACCGTTAATGGGTCACCATCGAGAAACCCTGCTGGTGGCAGCAACGGCCGAGCTGCTTCCAGATCCACTGCGGGGAAACGATCTCCTCCGCCGGAGAAAAATGAGAGAAAGGTGCCAGCTTGCTCAGGCAGGTCATCAGCAGCGGCCAAAGATGACAACAAGCCAAATGGAGCTCAAGCTGATCGTGGGAATCATGCTGATTCTAGTTTGTTGCAATCGGATCAGTTGGCCGTCGGGGCAGGGGCGGATAATTCTTCCGCCGCAGCTGCAACGGCTTCCGCGGCTGCAGCGGAGAAGGTGAACTACGAAGTGGTGGAGTGGCCGAGAATTTATATTGCTCTGTCGAGGAAGGAGAAGGAAGATGATTTCCTTGCAATGAAAGGCACAAAGCTCCCTCAGAGGCCCAAAAAAAGGGCCAAAAATGTTGACAGAACTCTGCAGTATTGTTTTCCAGGTATGTGGCTGTCTGACTTAACAAGGAATCGATATGAGGTCAGGGAGAAGAAATGTGTGAAGAAGCAAAAGCGAAGGGGACTCAAGGGAATGGACAGCGTCGACAGTGATTCCGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1639

Protein Analysis

235

Amino Acids

24.98

Weight (kDa)

10.0

Isoelectric Point (pI)

59.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 158 - 207 3.9e-23 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 687
AccII CGCG 1 cut(s) 413
AclWI GGATC 3 cut(s) 129, 189, 360
AcoI YGGCCR 4 cut(s) 178, 276, 360, 452
AcsI RAATTY 2 cut(s) 112, 459
AfiI CCNNNNNNNGG 4 cut(s) 141, 167, 204, 543
AjnI CCWGG 1 cut(s) 583
AluBI AGCT 6 cut(s) 185, 251, 305, 311, 398, 523
AluI AGCT 6 cut(s) 185, 251, 305, 311, 398, 523
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 3 cut(s) 129, 189, 360
AoxI GGCC 6 cut(s) 178, 276, 360, 452, 533, 546
ApeKI GCWGC 8 cut(s) 69, 171, 185, 272, 395, 398, 416, 419
ApoI RAATTY 2 cut(s) 112, 459
AspS9I GGNCC 2 cut(s) 534, 546
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 2 cut(s) 138, 442
BanI GGYRCC 1 cut(s) 244
BanII GRGCYC 1 cut(s) 307
BarI GAAGNNNNNNTAC 2 cut(s) 419, 451
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 8 cut(s) 81, 172, 183, 284, 385, 403, 407, 431
BccI CCATC 1 cut(s) 157
BceAI ACGGC 4 cut(s) 82, 193, 347, 420
BciT130I CCWGG 1 cut(s) 585
BcnI CCSGG 1 cut(s) 94
BfaI CTAG 2 cut(s) 105, 336
BfmI CTRYAG 2 cut(s) 417, 569
BfuAI ACCTGC 1 cut(s) 252
BglI GCCNNNNNGGC 1 cut(s) 368
Bme1390I CCNGG 2 cut(s) 94, 585
BmgT120I GGNCC 2 cut(s) 534, 546
BmiI GGNNCC 2 cut(s) 91, 246
BmrFI CCNGG 2 cut(s) 94, 585
BpiI GAAGAC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 256
BpuEI CTTGAG 2 cut(s) 291, 653
BpuMI CCSGG 1 cut(s) 94
Bsa29I ATCGAT 2 cut(s) 124, 616
BsaJI CCNNGG 1 cut(s) 411
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 167, 204, 543
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 1 cut(s) 585
BseCI ATCGAT 2 cut(s) 124, 616
BseDI CCNNGG 1 cut(s) 411
BseLI CCNNNNNNNGG 4 cut(s) 141, 167, 204, 543
BseMI GCAATG 1 cut(s) 513
BseMII CTCAG 3 cut(s) 68, 270, 542
BseRI GAGGAG 2 cut(s) 6, 207
BseX3I CGGCCG 1 cut(s) 178
BseXI GCAGC 8 cut(s) 81, 172, 183, 284, 385, 403, 407, 431
Bsh1236I CGCG 1 cut(s) 413
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 6 cut(s) 180, 278, 362, 454, 535, 548
BshNI GGYRCC 1 cut(s) 244
BshVI ATCGAT 2 cut(s) 124, 616
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 2 cut(s) 93, 224
BslFI GGGAC 1 cut(s) 678
BslI CCNNNNNNNGG 4 cut(s) 141, 167, 204, 543
BsmFI GGGAC 1 cut(s) 678
BsnI GGCC 6 cut(s) 180, 278, 362, 454, 535, 548
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 6 cut(s) 121, 125, 194, 212, 313, 352
BspANI GGCC 6 cut(s) 180, 278, 362, 454, 535, 548
BspCNI CTCAG 3 cut(s) 69, 269, 541
BspDI ATCGAT 2 cut(s) 124, 616
BspFNI CGCG 1 cut(s) 413
BspLI GGNNCC 2 cut(s) 91, 246
BspMAI CTGCAG 2 cut(s) 421, 573
BspMI ACCTGC 1 cut(s) 252
BspPI GGATC 3 cut(s) 129, 189, 360
BspT107I GGYRCC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 411
BssMI GATC 6 cut(s) 121, 125, 194, 212, 313, 352
Bst2UI CCWGG 1 cut(s) 585
Bst4CI ACNGT 2 cut(s) 136, 692
BstC8I GCNNGC 2 cut(s) 249, 253
BstDEI CTNAG 3 cut(s) 77, 256, 528
BstDSI CCRYGG 1 cut(s) 411
BstEII GGTNACC 1 cut(s) 144
BstFNI CGCG 1 cut(s) 413
BstKTI GATC 6 cut(s) 124, 128, 197, 215, 316, 355
BstMBI GATC 6 cut(s) 121, 125, 194, 212, 313, 352
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 6 cut(s) 177, 368, 398, 404, 413, 419
BstNI CCWGG 1 cut(s) 585
BstPI GGTNACC 1 cut(s) 144
BstSCI CCNGG 2 cut(s) 92, 583
BstSFI CTRYAG 2 cut(s) 417, 569
BstUI CGCG 1 cut(s) 413
BstV1I GCAGC 8 cut(s) 81, 172, 183, 284, 385, 403, 407, 431
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 194
BstXI CCANNNNNNTGG 1 cut(s) 591
BstYI RGATCY 1 cut(s) 194
BstZI CGGCCG 1 cut(s) 178
Bsu15I ATCGAT 2 cut(s) 124, 616
BsuRI GGCC 6 cut(s) 180, 278, 362, 454, 535, 548
BsuTUI ATCGAT 2 cut(s) 124, 616
BtgI CCRYGG 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 198, 697
BveI ACCTGC 1 cut(s) 252
Cac8I GCNNGC 2 cut(s) 249, 253
Cfr13I GGNCC 2 cut(s) 534, 546
Cfr42I CCGCGG 1 cut(s) 414
ClaI ATCGAT 2 cut(s) 124, 616
CseI GACGC 1 cut(s) 673
CviAII CATG 1 cut(s) 326
DdeI CTNAG 3 cut(s) 77, 256, 528
DpnI GATC 6 cut(s) 123, 127, 196, 214, 315, 354
DpnII GATC 6 cut(s) 121, 125, 194, 212, 313, 352
EaeI YGGCCR 4 cut(s) 178, 276, 360, 452
EagI CGGCCG 1 cut(s) 178
EciI GGCGGA 4 cut(s) 9, 210, 379, 392
Ecl136II GAGCTC 1 cut(s) 305
EclXI CGGCCG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 307
Eco52I CGGCCG 1 cut(s) 178
Eco53kI GAGCTC 1 cut(s) 305
Eco91I GGTNACC 1 cut(s) 144
EcoICRI GAGCTC 1 cut(s) 305
EcoO65I GGTNACC 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 583
EcoT38I GRGCYC 1 cut(s) 307
FaeI CATG 1 cut(s) 329
FaiI YATR 4 cut(s) 327, 465, 590, 621
FaqI GGGAC 1 cut(s) 678
FatI CATG 1 cut(s) 325
FauI CCCGC 1 cut(s) 196
FblI GTMKAC 1 cut(s) 687
FriOI GRGCYC 1 cut(s) 307
FspBI CTAG 2 cut(s) 105, 336
HaeIII GGCC 6 cut(s) 180, 278, 362, 454, 535, 548
HapII CCGG 2 cut(s) 93, 224
HgaI GACGC 1 cut(s) 673
Hin1II CATG 1 cut(s) 329
HincII GTYRAC 2 cut(s) 559, 688
HindII GTYRAC 2 cut(s) 559, 688
HinfI GANTC 5 cut(s) 322, 332, 613, 666, 695
HpaII CCGG 2 cut(s) 93, 224
HphI GGTGA 2 cut(s) 138, 442
Hpy166II GTNNAC 3 cut(s) 433, 559, 688
Hpy188I TCNGA 4 cut(s) 352, 531, 601, 700
Hpy188III TCNNGA 2 cut(s) 153, 192
Hpy8I GTNNAC 3 cut(s) 433, 559, 688
Hpy99I CGWCG 4 cut(s) 55, 58, 368, 689
HpyAV CCTTC 6 cut(s) 38, 80, 421, 475, 481, 654
HpyCH4III ACNGT 2 cut(s) 136, 692
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 5 cut(s) 346, 401, 419, 506, 571
HpyF10VI GCNNNNNNNGC 6 cut(s) 177, 368, 398, 404, 413, 419
HpyF3I CTNAG 3 cut(s) 77, 256, 528
HpySE526I ACGT 1 cut(s) 50
Hsp92II CATG 1 cut(s) 329
KspI CCGCGG 1 cut(s) 414
Kzo9I GATC 6 cut(s) 121, 125, 194, 212, 313, 352
LmnI GCTCC 3 cut(s) 95, 302, 528
Lsp1109I GCAGC 8 cut(s) 81, 172, 183, 284, 385, 403, 407, 431
MaeI CTAG 2 cut(s) 105, 336
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 144
MalI GATC 6 cut(s) 123, 127, 196, 214, 315, 354
MboI GATC 6 cut(s) 121, 125, 194, 212, 313, 352
MboII GAAGA 6 cut(s) 27, 30, 378, 503, 646, 658
MflI RGATCY 1 cut(s) 194
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 3 cut(s) 112, 382, 459
MlyI GAGTC 1 cut(s) 660
MmeI TCCRAC 1 cut(s) 87
MnlI CCTC 9 cut(s) 21, 24, 27, 52, 228, 471, 525, 537, 616
MseI TTAA 2 cut(s) 138, 605
MspA1I CMGCKG 4 cut(s) 275, 398, 413, 422
MspI CCGG 2 cut(s) 93, 224
MspR9I CCNGG 2 cut(s) 94, 585
MvaI CCWGG 1 cut(s) 585
MvnI CGCG 1 cut(s) 413
MwoI GCNNNNNNNGC 6 cut(s) 177, 368, 398, 404, 413, 419
NciI CCSGG 1 cut(s) 94
NdeII GATC 6 cut(s) 121, 125, 194, 212, 313, 352
NlaIII CATG 1 cut(s) 329
NlaIV GGNNCC 2 cut(s) 91, 246
NmeAIII GCCGAG 2 cut(s) 206, 480
NmuCI GTSAC 1 cut(s) 144
PfeI GAWTC 4 cut(s) 322, 332, 613, 695
PflFI GACNNNGTC 1 cut(s) 683
PleI GAGTC 1 cut(s) 660
PpsI GAGTC 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 583
PspEI GGTNACC 1 cut(s) 144
PspGI CCWGG 1 cut(s) 583
PspN4I GGNNCC 2 cut(s) 91, 246
PspPI GGNCC 2 cut(s) 534, 546
PstI CTGCAG 2 cut(s) 421, 573
PsuI RGATCY 1 cut(s) 194
PsyI GACNNNGTC 1 cut(s) 683
PvuII CAGCTG 1 cut(s) 398
SacI GAGCTC 1 cut(s) 307
SacII CCGCGG 1 cut(s) 414
SalI GTCGAC 1 cut(s) 686
SaqAI TTAA 2 cut(s) 138, 605
Sau3AI GATC 6 cut(s) 121, 125, 194, 212, 313, 352
Sau96I GGNCC 2 cut(s) 534, 546
SchI GAGTC 1 cut(s) 660
ScrFI CCNGG 2 cut(s) 94, 585
SduI GDGCHC 1 cut(s) 307
SfcI CTRYAG 2 cut(s) 417, 569
Sfr303I CCGCGG 1 cut(s) 414
SgrBI CCGCGG 1 cut(s) 414
SmlI CTYRAG 2 cut(s) 306, 668
SmoI CTYRAG 2 cut(s) 306, 668
Sse9I AATT 3 cut(s) 112, 382, 459
SspMI CTAG 2 cut(s) 105, 336
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 2 cut(s) 92, 583
TaaI ACNGT 2 cut(s) 136, 692
TaiI ACGT 1 cut(s) 53
TaqI TCGA 5 cut(s) 124, 152, 476, 616, 687
TasI AATT 3 cut(s) 112, 382, 459
TauI GCSGC 6 cut(s) 7, 10, 25, 278, 395, 416
TfiI GAWTC 4 cut(s) 322, 332, 613, 695
Tru1I TTAA 2 cut(s) 138, 605
Tru9I TTAA 2 cut(s) 138, 605
TscAI CASTG 2 cut(s) 205, 697
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 8 cut(s) 69, 171, 185, 272, 395, 398, 416, 419
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 1 cut(s) 524
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 2 cut(s) 205, 697
Tth111I GACNNNGTC 1 cut(s) 683
XapI RAATTY 2 cut(s) 112, 459
XmiI GTMKAC 1 cut(s) 687
XspI CTAG 2 cut(s) 105, 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.