pycom05g23120

Phosphatase IMPL1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002951
Physical Location & Seq
Forward (+)
25138828 .. 25141647
2820 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
ATGGGCAGGTCCCTGGTTTTCTCCACAAACGTTCCTCTCAGAATTTCTCAAATACCCAGATCAATTTTACCTCCCAATCGACCAGGTTACTCACTTTTTCTGAATCCAAAGCAAAAATTGCAACATGGGTTTCAAAGATTTGGACTTTCGATTGCCAAACCGACGAGAAAACTCTGCACAAAGGCCGTGTTGTCTGGAATTCCAAACCACGAGCTGTATGTGAAAGTTGGCGCCAAATCAACCGGACCAATTCCAATTGGTCAGCTCGTTGGAGTGGTTGAGAAGGCCGCTAAGACTGGCGCTGAGGTTGTGATGAATGCTGTTAATAAGCCTCGGAATATCACCTATAAAGGAGTAGCGGACTTGGTCACTGACACAGATAAAAAGAGTGAAGCCACAATTTTAGAAGTTCTGAGAAAGAACTTTGAAGATCACCTTATTCTTGGAGAGGAGGGTGGAATAACTGGGGATGCATCATCTGATTATATGTGGTGCATCGATCCTTTAGATGGAACAACAAATTTCGCACATGGTTATCCTAGCTTTGCAGTTTCTGTTGGAGTTTTATTTCGAGGAAAACCTGTTGCTGCTGCCGTGGTTGAGTTCGTTGGGGGCCCTATGTGTTGGAATACCCGCATTTTTTCTGCTAGTGCTGGAGGGGGAGCATTTTGTAATGGCCAAAAAATTCATGTGAGCCAAACTGATGAGGTGGGACGGTCTCTTCTTGTTACTGGATTTGGTTATGAACATGATGACGTATGGATCGACCAACATAGAGTTATTTAA

Protein Analysis

262

Amino Acids

28.21

Weight (kDa)

9.0

Isoelectric Point (pI)

22.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inositol_P PF00459 93 - 254 1.5e-43 Inositol monophosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 230
AciI CCGC 3 cut(s) 288, 359, 634
AclI AACGTT 1 cut(s) 30
AclWI GGATC 2 cut(s) 494, 770
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 4 cut(s) 42, 198, 520, 684
AcyI GRCGYC 1 cut(s) 231
AfiI CCNNNNNNNGG 1 cut(s) 509
AgsI TTSAA 2 cut(s) 134, 428
AjnI CCWGG 2 cut(s) 12, 82
AluBI AGCT 3 cut(s) 214, 265, 543
AluI AGCT 3 cut(s) 214, 265, 543
Alw26I GTCTC 1 cut(s) 723
AlwI GGATC 2 cut(s) 494, 770
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 4 cut(s) 183, 285, 613, 676
ApaI GGGCCC 1 cut(s) 617
ApeKI GCWGC 2 cut(s) 587, 590
ApoI RAATTY 4 cut(s) 42, 198, 520, 684
AspLEI GCGC 2 cut(s) 233, 302
AspS9I GGNCC 4 cut(s) 9, 245, 613, 614
AsuHPI GGTGA 2 cut(s) 334, 425
AvaII GGWCC 2 cut(s) 9, 245
BaeGI GKGCMC 1 cut(s) 617
BalI TGGCCA 1 cut(s) 678
BanI GGYRCC 1 cut(s) 230
BanII GRGCYC 1 cut(s) 617
BauI CACGAG 1 cut(s) 209
BbvCI CCTCAGC 1 cut(s) 303
BbvI GCAGC 2 cut(s) 574, 577
BccI CCATC 1 cut(s) 503
BceAI ACGGC 2 cut(s) 170, 578
BciT130I CCWGG 2 cut(s) 14, 84
BcoDI GTCTC 1 cut(s) 723
BfaI CTAG 2 cut(s) 540, 648
BfoI RGCGCY 2 cut(s) 234, 303
BisI GCNGC 3 cut(s) 288, 588, 591
BlsI GCNGC 3 cut(s) 289, 589, 592
Bme1390I CCNGG 2 cut(s) 14, 84
Bme18I GGWCC 2 cut(s) 9, 245
BmgT120I GGNCC 4 cut(s) 9, 245, 613, 614
BmiI GGNNCC 4 cut(s) 11, 232, 614, 615
BmrFI CCNGG 2 cut(s) 14, 84
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 3 cut(s) 460, 482, 504
BmuI ACTGGG 1 cut(s) 474
BpmI CTGGAG 1 cut(s) 675
Bpu10I CCTNAGC 1 cut(s) 303
Bsa29I ATCGAT 1 cut(s) 498
BsaHI GRCGYC 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 723
BsaJI CCNNGG 3 cut(s) 12, 332, 594
BsaWI WCCGGW 1 cut(s) 242
Bsc4I CCNNNNNNNGG 1 cut(s) 509
Bse1I ACTGG 3 cut(s) 301, 469, 736
BseBI CCWGG 2 cut(s) 14, 84
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 3 cut(s) 12, 332, 594
BseGI GGATG 1 cut(s) 475
BseLI CCNNNNNNNGG 1 cut(s) 509
BseMII CTCAG 3 cut(s) 52, 294, 404
BseNI ACTGG 3 cut(s) 301, 469, 736
BseRI GAGGAG 1 cut(s) 464
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 2 cut(s) 574, 577
BsgI GTGCAG 1 cut(s) 160
BshFI GGCC 4 cut(s) 185, 287, 615, 678
BshNI GGYRCC 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 498
BsiSI CCGG 1 cut(s) 243
BslFI GGGAC 1 cut(s) 726
BslI CCNNNNNNNGG 1 cut(s) 509
BsmAI GTCTC 1 cut(s) 723
BsmFI GGGAC 1 cut(s) 726
BsmI GAATGC 1 cut(s) 322
BsnI GGCC 4 cut(s) 185, 287, 615, 678
Bso31I GGTCTC 1 cut(s) 723
Bsp120I GGGCCC 1 cut(s) 613
Bsp1286I GDGCHC 1 cut(s) 617
Bsp143I GATC 4 cut(s) 59, 430, 499, 762
BspACI CCGC 3 cut(s) 288, 359, 634
BspANI GGCC 4 cut(s) 185, 287, 615, 678
BspCNI CTCAG 3 cut(s) 51, 295, 405
BspDI ATCGAT 1 cut(s) 498
BspLI GGNNCC 4 cut(s) 11, 232, 614, 615
BspPI GGATC 2 cut(s) 494, 770
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 723
BsrI ACTGG 3 cut(s) 301, 469, 736
BssECI CCNNGG 3 cut(s) 12, 332, 594
BssMI GATC 4 cut(s) 59, 430, 499, 762
BssNI GRCGYC 1 cut(s) 231
BssSI CACGAG 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 2 cut(s) 14, 84
Bst4CI ACNGT 1 cut(s) 717
Bst6I CTCTTC 1 cut(s) 726
BstACI GRCGYC 1 cut(s) 231
BstAPI GCANNNNNTGC 1 cut(s) 118
BstDEI CTNAG 4 cut(s) 38, 291, 303, 413
BstDSI CCRYGG 1 cut(s) 594
BstF5I GGATG 1 cut(s) 475
BstH2I RGCGCY 2 cut(s) 234, 303
BstHHI GCGC 2 cut(s) 233, 302
BstKTI GATC 4 cut(s) 62, 433, 502, 765
BstMAI GTCTC 1 cut(s) 723
BstMBI GATC 4 cut(s) 59, 430, 499, 762
BstMWI GCNNNNNNNGC 1 cut(s) 118
BstNI CCWGG 2 cut(s) 14, 84
BstSCI CCNGG 2 cut(s) 12, 82
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 2 cut(s) 574, 577
Bsu15I ATCGAT 1 cut(s) 498
BsuRI GGCC 4 cut(s) 185, 287, 615, 678
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 594
BtsCI GGATG 1 cut(s) 475
BtsIMutI CAGTG 1 cut(s) 369
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 2 cut(s) 233, 302
Cfr13I GGNCC 4 cut(s) 9, 245, 613, 614
ClaI ATCGAT 1 cut(s) 498
CsiI ACCWGGT 1 cut(s) 82
CviAII CATG 4 cut(s) 125, 530, 689, 749
DdeI CTNAG 4 cut(s) 38, 291, 303, 413
DinI GGCGCC 1 cut(s) 232
DpnI GATC 4 cut(s) 61, 432, 501, 764
DpnII GATC 4 cut(s) 59, 430, 499, 762
EaeI YGGCCR 1 cut(s) 676
Eam1104I CTCTTC 1 cut(s) 726
EarI CTCTTC 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 617
Eco31I GGTCTC 1 cut(s) 723
Eco47I GGWCC 2 cut(s) 9, 245
EcoO109I RGGNCCY 3 cut(s) 9, 613, 614
EcoRI GAATTC 1 cut(s) 198
EcoRII CCWGG 2 cut(s) 12, 82
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 617
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
FaeI CATG 4 cut(s) 128, 533, 692, 752
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 1 cut(s) 726
FatI CATG 4 cut(s) 124, 529, 688, 748
FauI CCCGC 1 cut(s) 641
Fnu4HI GCNGC 3 cut(s) 288, 588, 591
FokI GGATG 1 cut(s) 482
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 3 cut(s) 288, 588, 591
FspBI CTAG 2 cut(s) 540, 648
GlaI GCGC 2 cut(s) 232, 301
GluI GCNGC 3 cut(s) 288, 588, 591
GsuI CTGGAG 1 cut(s) 675
HaeII RGCGCY 2 cut(s) 234, 303
HaeIII GGCC 4 cut(s) 185, 287, 615, 678
HapII CCGG 1 cut(s) 243
HhaI GCGC 2 cut(s) 233, 302
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 4 cut(s) 128, 533, 692, 752
Hin6I GCGC 2 cut(s) 231, 300
HinP1I GCGC 2 cut(s) 231, 300
HinfI GANTC 1 cut(s) 103
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 334, 425
Hpy188I TCNGA 5 cut(s) 41, 102, 336, 414, 481
Hpy188III TCNNGA 1 cut(s) 195
Hpy99I CGWCG 1 cut(s) 166
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 1 cut(s) 717
HpyCH4IV ACGT 2 cut(s) 30, 756
HpyCH4V TGCA 5 cut(s) 121, 177, 473, 495, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 118
HpyF3I CTNAG 4 cut(s) 38, 291, 303, 413
HpySE526I ACGT 2 cut(s) 30, 756
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 4 cut(s) 128, 533, 692, 752
HspAI GCGC 2 cut(s) 231, 300
KasI GGCGCC 1 cut(s) 230
Kzo9I GATC 4 cut(s) 59, 430, 499, 762
LmnI GCTCC 1 cut(s) 662
Lsp1109I GCAGC 2 cut(s) 574, 577
LweI GCATC 3 cut(s) 460, 482, 504
MabI ACCWGGT 1 cut(s) 82
MaeI CTAG 2 cut(s) 540, 648
MaeII ACGT 2 cut(s) 30, 756
MaeIII GTNAC 3 cut(s) 86, 367, 727
MalI GATC 4 cut(s) 61, 432, 501, 764
MboI GATC 4 cut(s) 59, 430, 499, 762
MboII GAAGA 2 cut(s) 440, 713
MfeI CAATTG 1 cut(s) 255
MhlI GDGCHC 1 cut(s) 617
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 9 cut(s) 42, 63, 116, 198, 249, 255, 399, 520, 684
MluNI TGGCCA 1 cut(s) 678
Mly113I GGCGCC 1 cut(s) 231
MmeI TCCRAC 3 cut(s) 250, 538, 605
MnlI CCTC 9 cut(s) 45, 81, 298, 342, 442, 445, 566, 650, 700
Mox20I TGGCCA 1 cut(s) 678
Mph1103I ATGCAT 1 cut(s) 475
MscI TGGCCA 1 cut(s) 678
MseI TTAA 2 cut(s) 324, 784
Msp20I TGGCCA 1 cut(s) 678
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 14, 84
MunI CAATTG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 322
MvaI CCWGG 2 cut(s) 14, 84
MwoI GCNNNNNNNGC 1 cut(s) 118
NarI GGCGCC 1 cut(s) 231
NdeII GATC 4 cut(s) 59, 430, 499, 762
NlaIII CATG 4 cut(s) 128, 533, 692, 752
NlaIV GGNNCC 4 cut(s) 11, 232, 614, 615
NmuCI GTSAC 1 cut(s) 367
NsiI ATGCAT 1 cut(s) 475
PcsI WCGNNNNNNNCGW 1 cut(s) 762
PctI GAATGC 1 cut(s) 322
PfeI GAWTC 1 cut(s) 103
PflFI GACNNNGTC 1 cut(s) 365
PkrI GCNGC 3 cut(s) 289, 589, 592
PluTI GGCGCC 1 cut(s) 234
PpuMI RGGWCCY 1 cut(s) 9
Psp1406I AACGTT 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 9
Psp6I CCWGG 2 cut(s) 12, 82
PspGI CCWGG 2 cut(s) 12, 82
PspN4I GGNNCC 4 cut(s) 11, 232, 614, 615
PspOMI GGGCCC 1 cut(s) 613
PspPI GGNCC 4 cut(s) 9, 245, 613, 614
PspPPI RGGWCCY 1 cut(s) 9
PstNI CAGNNNCTG 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 365
SaqAI TTAA 2 cut(s) 324, 784
SatI GCNGC 3 cut(s) 288, 588, 591
Sau3AI GATC 4 cut(s) 59, 430, 499, 762
Sau96I GGNCC 4 cut(s) 9, 245, 613, 614
ScrFI CCNGG 2 cut(s) 14, 84
SduI GDGCHC 1 cut(s) 617
SexAI ACCWGGT 1 cut(s) 82
SfaNI GCATC 3 cut(s) 460, 482, 504
SfoI GGCGCC 1 cut(s) 232
SinI GGWCC 2 cut(s) 9, 245
Sse9I AATT 9 cut(s) 42, 63, 116, 198, 249, 255, 399, 520, 684
SsiI CCGC 3 cut(s) 288, 359, 634
SspDI GGCGCC 1 cut(s) 230
SspMI CTAG 2 cut(s) 540, 648
StyD4I CCNGG 2 cut(s) 12, 82
TaaI ACNGT 1 cut(s) 717
TaiI ACGT 2 cut(s) 33, 759
TaqI TCGA 5 cut(s) 79, 149, 498, 571, 765
TasI AATT 9 cut(s) 42, 63, 116, 198, 249, 255, 399, 520, 684
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 324, 784
Tru9I TTAA 2 cut(s) 324, 784
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 2 cut(s) 587, 590
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 3 cut(s) 329, 677, 759
TspRI CASTG 1 cut(s) 376
Tth111I GACNNNGTC 1 cut(s) 365
VpaK11BI GGWCC 2 cut(s) 9, 245
XapI RAATTY 4 cut(s) 42, 198, 520, 684
XspI CTAG 2 cut(s) 540, 648
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.