pycom05g31930

ribosomal protein L4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002609
Physical Location & Seq
Reverse (-)
31459882 .. 31462591
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 975 bp
ATGGCTTTGTCGGTATCCAGAAGGCTTTTGCGTTCATTTGGTTCTGTATTGGCATCAGGCCGCTGTGATTCTTCAACTAATTTATATCCATCATCATTTCGTGCTTCTCTCGATTTTTGCGGCCATGCTCCCGGGGATACCTTACTTTCTGGAGAATTGTCAGCTGTTTTCAAGCCTGGATCATTTTTTCTAGCAAATCGAAGGCTTTCAACCTCCAACCCAACTCCCGAATCAAGTGGAGGTGCCTTTCCCTCTAATTTATTGTCAGCAAAGCCTGTGGTTGCATCAGAGCGTTCTATAGGGCTCTGTCAAGACCTTGTGATTCCAGTGACAAATTTTCATTATGAAGATAAAGGCTTCACAGTTTTGGCTGGTGATGTTTTCGATGTACCTATTAGGAAGGATATTATTCATCGGGTTGTCCTATGGCAGCTAGCAAAACGGCAACAGGGAACACATTCAACAAAAACTATTAGTGAGGTTAGTGGGACTGGGAGAAAGCCATGGAATCAGAAGGGTACTGGTCGAGCGAGGCATGGAACATTACGTGGTCCCCAGTTCAGAGGAGGATGCACAATGCATGGTCCTAAGCCGCGAAGCCATGAAATTAAGCTGAATAAGAAGGTTCGGCGTCTAGGACTAAAGATTGCTCTGACAGCTCAAGCAGCAGAGGGAAAGCTTCTGGTTTTTGAAGATTTTGAGGTCCCCACGCATAAAACCAAGAATATTGTAAACTACGTCCAGCAAATGGAGGGCTCCAAGAAATTTTTGCTGGTGGATGGTACTAAAACAGAGGATGGTAAACAAATGTTACCCGAAAAGCTCAAGTTGGCTACACAAAATTTGCATTATGTGAACGTGCTGCCCGCAATTGGTTTGAACGTCTACAGCATCCTGCAGCATGACACACTGGTGATGACCCGAGCAGCTGTAAATGAAATTGTCAAGCGGATGCACACTCCAATCAACCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L4

Protein Analysis

325

Amino Acids

35.65

Weight (kDa)

10.07

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L4 PF00573 123 - 316 7.3e-69 Ribosomal protein L4/L1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 242
AccB7I CCANNNNNTGG 1 cut(s) 748
AccI GTMKAC 1 cut(s) 885
AccII CGCG 1 cut(s) 595
AciI CCGC 5 cut(s) 61, 120, 593, 867, 949
AclWI GGATC 1 cut(s) 187
AcoI YGGCCR 1 cut(s) 121
AcsI RAATTY 3 cut(s) 334, 764, 841
AcyI GRCGYC 1 cut(s) 631
AfaI GTAC 3 cut(s) 390, 520, 784
AfiI CCNNNNNNNGG 2 cut(s) 748, 872
AgsI TTSAA 6 cut(s) 75, 172, 210, 462, 692, 880
AjnI CCWGG 1 cut(s) 175
AleI CACNNNNGTG 1 cut(s) 911
AluBI AGCT 7 cut(s) 164, 433, 613, 659, 679, 823, 929
AluI AGCT 7 cut(s) 164, 433, 613, 659, 679, 823, 929
AlwI GGATC 1 cut(s) 187
Ama87I CYCGRG 2 cut(s) 131, 921
AoxI GGCC 2 cut(s) 58, 121
ApeKI GCWGC 5 cut(s) 430, 665, 862, 898, 926
ApoI RAATTY 3 cut(s) 334, 764, 841
Asp700I GAANNNNTTC 2 cut(s) 205, 457
AspS9I GGNCC 3 cut(s) 551, 584, 703
AsuC2I CCSGG 2 cut(s) 132, 133
AsuHPI GGTGA 2 cut(s) 386, 925
AsuNHI GCTAGC 1 cut(s) 433
AvaI CYCGRG 2 cut(s) 131, 921
AvaII GGWCC 3 cut(s) 551, 584, 703
BanI GGYRCC 1 cut(s) 242
BanII GRGCYC 2 cut(s) 306, 758
BbvI GCAGC 5 cut(s) 442, 677, 849, 910, 938
BccI CCATC 3 cut(s) 97, 773, 791
BceAI ACGGC 1 cut(s) 458
BciT130I CCWGG 1 cut(s) 177
BciVI GTATCC 2 cut(s) 25, 130
BcnI CCSGG 2 cut(s) 132, 133
BfaI CTAG 3 cut(s) 191, 434, 635
BfmI CTRYAG 3 cut(s) 297, 886, 896
BfuI GTATCC 2 cut(s) 25, 130
BisI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 863, 899, 927
BlsI GCNGC 8 cut(s) 62, 122, 432, 594, 667, 864, 900, 928
Bme1390I CCNGG 3 cut(s) 132, 133, 177
Bme18I GGWCC 3 cut(s) 551, 584, 703
BmeT110I CYCGRG 2 cut(s) 131, 921
BmgT120I GGNCC 3 cut(s) 551, 584, 703
BmiI GGNNCC 4 cut(s) 244, 553, 705, 757
BmrFI CCNGG 3 cut(s) 132, 133, 177
BmrI ACTGGG 2 cut(s) 501, 550
BmsI GCATC 5 cut(s) 62, 293, 560, 900, 942
BmtI GCTAGC 1 cut(s) 437
BmuI ACTGGG 2 cut(s) 501, 550
BpmI CTGGAG 1 cut(s) 171
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 645, 809
BpuMI CCSGG 2 cut(s) 132, 133
BsaAI YACGTR 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 631
BsaJI CCNNGG 3 cut(s) 131, 132, 503
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 872
Bse1I ACTGG 5 cut(s) 326, 496, 526, 556, 915
BseBI CCWGG 1 cut(s) 177
BseDI CCNNGG 3 cut(s) 131, 132, 503
BseGI GGATG 5 cut(s) 575, 784, 802, 891, 957
BseLI CCNNNNNNNGG 2 cut(s) 748, 872
BseNI ACTGG 5 cut(s) 326, 496, 526, 556, 915
BseRI GAGGAG 1 cut(s) 579
BseXI GCAGC 5 cut(s) 442, 677, 849, 910, 938
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 2 cut(s) 60, 123
BshNI GGYRCC 1 cut(s) 242
BsiHKCI CYCGRG 2 cut(s) 131, 921
BsiSI CCGG 1 cut(s) 132
BslFI GGGAC 3 cut(s) 502, 537, 689
BslI CCNNNNNNNGG 2 cut(s) 748, 872
BsmFI GGGAC 3 cut(s) 502, 537, 689
BsnI GGCC 2 cut(s) 60, 123
BsoBI CYCGRG 2 cut(s) 131, 921
Bsp1286I GDGCHC 2 cut(s) 306, 758
Bsp143I GATC 1 cut(s) 179
Bsp19I CCATGG 1 cut(s) 503
BspACI CCGC 5 cut(s) 61, 120, 593, 867, 949
BspANI GGCC 2 cut(s) 60, 123
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 4 cut(s) 244, 553, 705, 757
BspMAI CTGCAG 1 cut(s) 900
BspOI GCTAGC 1 cut(s) 437
BspPI GGATC 1 cut(s) 187
BspT107I GGYRCC 1 cut(s) 242
BsrI ACTGG 5 cut(s) 326, 496, 526, 556, 915
BssECI CCNNGG 3 cut(s) 131, 132, 503
BssMI GATC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 631
BssT1I CCWWGG 1 cut(s) 503
Bst2UI CCWGG 1 cut(s) 177
Bst4CI ACNGT 2 cut(s) 364, 971
BstACI GRCGYC 1 cut(s) 631
BstBAI YACGTR 1 cut(s) 548
BstC8I GCNNGC 2 cut(s) 435, 867
BstDEI CTNAG 1 cut(s) 588
BstDSI CCRYGG 1 cut(s) 503
BstF5I GGATG 5 cut(s) 575, 784, 802, 891, 957
BstFNI CGCG 1 cut(s) 595
BstKTI GATC 1 cut(s) 182
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 2 cut(s) 656, 665
BstNI CCWGG 1 cut(s) 177
BstSCI CCNGG 3 cut(s) 130, 131, 175
BstSFI CTRYAG 3 cut(s) 297, 886, 896
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 5 cut(s) 442, 677, 849, 910, 938
BsuI GTATCC 2 cut(s) 25, 130
BsuRI GGCC 2 cut(s) 60, 123
BtgI CCRYGG 1 cut(s) 503
BtsCI GGATG 5 cut(s) 575, 784, 802, 891, 957
BtsIMutI CAGTG 2 cut(s) 333, 908
Cac8I GCNNGC 2 cut(s) 435, 867
Cfr13I GGNCC 3 cut(s) 551, 584, 703
Cfr9I CCCGGG 1 cut(s) 131
CseI GACGC 1 cut(s) 620
Csp6I GTAC 3 cut(s) 389, 519, 783
CspCI CAANNNNNGTGG 2 cut(s) 258, 293
CviAII CATG 6 cut(s) 125, 504, 536, 581, 602, 902
CviQI GTAC 3 cut(s) 389, 519, 783
DdeI CTNAG 1 cut(s) 588
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 503
Eco24I GRGCYC 2 cut(s) 306, 758
Eco47I GGWCC 3 cut(s) 551, 584, 703
Eco88I CYCGRG 2 cut(s) 131, 921
EcoO109I RGGNCCY 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 503
EcoT22I ATGCAT 1 cut(s) 582
EcoT38I GRGCYC 2 cut(s) 306, 758
ErhI CCWWGG 1 cut(s) 503
FaeI CATG 6 cut(s) 128, 507, 539, 584, 605, 905
FaqI GGGAC 3 cut(s) 502, 537, 689
FatI CATG 6 cut(s) 124, 503, 535, 580, 601, 901
FauI CCCGC 1 cut(s) 874
FblI GTMKAC 1 cut(s) 885
Fnu4HI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 863, 899, 927
FokI GGATG 5 cut(s) 582, 791, 809, 878, 964
FriOI GRGCYC 2 cut(s) 306, 758
Fsp4HI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 863, 899, 927
FspBI CTAG 3 cut(s) 191, 434, 635
GluI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 863, 899, 927
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 60, 123
HapII CCGG 1 cut(s) 132
HgaI GACGC 1 cut(s) 620
Hin1I GRCGYC 1 cut(s) 631
Hin1II CATG 6 cut(s) 128, 507, 539, 584, 605, 905
HindIII AAGCTT 1 cut(s) 677
HinfI GANTC 4 cut(s) 68, 230, 322, 508
HpaII CCGG 1 cut(s) 132
HphI GGTGA 2 cut(s) 386, 925
Hpy166II GTNNAC 4 cut(s) 733, 803, 856, 886
Hpy188I TCNGA 4 cut(s) 289, 513, 563, 654
Hpy188III TCNNGA 5 cut(s) 18, 110, 150, 227, 311
Hpy8I GTNNAC 4 cut(s) 733, 803, 856, 886
HpyAV CCTTC 5 cut(s) 15, 195, 394, 508, 616
HpyCH4III ACNGT 2 cut(s) 364, 971
HpyCH4IV ACGT 4 cut(s) 547, 738, 858, 882
HpyCH4V TGCA 6 cut(s) 284, 573, 580, 847, 898, 955
HpyF10VI GCNNNNNNNGC 2 cut(s) 656, 665
HpyF3I CTNAG 1 cut(s) 588
HpySE526I ACGT 4 cut(s) 547, 738, 858, 882
Hsp92I GRCGYC 1 cut(s) 631
Hsp92II CATG 6 cut(s) 128, 507, 539, 584, 605, 905
Kzo9I GATC 1 cut(s) 179
LmnI GCTCC 2 cut(s) 133, 761
Lsp1109I GCAGC 5 cut(s) 442, 677, 849, 910, 938
LweI GCATC 5 cut(s) 62, 293, 560, 900, 942
MaeI CTAG 3 cut(s) 191, 434, 635
MaeII ACGT 4 cut(s) 547, 738, 858, 882
MaeIII GTNAC 2 cut(s) 328, 810
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MboII GAAGA 3 cut(s) 63, 359, 704
MfeI CAATTG 1 cut(s) 870
MhlI GDGCHC 2 cut(s) 306, 758
MluCI AATT 9 cut(s) 79, 155, 256, 334, 606, 764, 841, 870, 939
MmeI TCCRAC 1 cut(s) 240
Mph1103I ATGCAT 1 cut(s) 582
MroXI GAANNNNTTC 2 cut(s) 205, 457
MseI TTAA 1 cut(s) 609
MslI CAYNNNNRTG 1 cut(s) 911
MspA1I CMGCKG 3 cut(s) 63, 164, 929
MspI CCGG 1 cut(s) 132
MspR9I CCNGG 3 cut(s) 132, 133, 177
MunI CAATTG 1 cut(s) 870
MvaI CCWGG 1 cut(s) 177
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 2 cut(s) 656, 665
NciI CCSGG 2 cut(s) 132, 133
NcoI CCATGG 1 cut(s) 503
NdeII GATC 1 cut(s) 179
NheI GCTAGC 1 cut(s) 433
NlaIII CATG 6 cut(s) 128, 507, 539, 584, 605, 905
NlaIV GGNNCC 4 cut(s) 244, 553, 705, 757
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 582
OliI CACNNNNGTG 1 cut(s) 911
PdmI GAANNNNTTC 2 cut(s) 205, 457
PfeI GAWTC 4 cut(s) 68, 230, 322, 508
PflMI CCANNNNNTGG 1 cut(s) 748
PkrI GCNGC 8 cut(s) 62, 122, 432, 594, 667, 864, 900, 928
Ppu21I YACGTR 1 cut(s) 548
PpuMI RGGWCCY 1 cut(s) 703
Psp5II RGGWCCY 1 cut(s) 703
Psp6I CCWGG 1 cut(s) 175
PspGI CCWGG 1 cut(s) 175
PspN4I GGNNCC 4 cut(s) 244, 553, 705, 757
PspPI GGNCC 3 cut(s) 551, 584, 703
PspPPI RGGWCCY 1 cut(s) 703
PstI CTGCAG 1 cut(s) 900
PvuII CAGCTG 2 cut(s) 164, 929
RsaI GTAC 3 cut(s) 390, 520, 784
RsaNI GTAC 3 cut(s) 389, 519, 783
RseI CAYNNNNRTG 1 cut(s) 911
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 8 cut(s) 61, 121, 431, 593, 666, 863, 899, 927
Sau3AI GATC 1 cut(s) 179
Sau96I GGNCC 3 cut(s) 551, 584, 703
ScrFI CCNGG 3 cut(s) 132, 133, 177
SduI GDGCHC 2 cut(s) 306, 758
SfaNI GCATC 5 cut(s) 62, 293, 560, 900, 942
SfcI CTRYAG 3 cut(s) 297, 886, 896
SinI GGWCC 3 cut(s) 551, 584, 703
SmaI CCCGGG 1 cut(s) 133
SmiMI CAYNNNNRTG 1 cut(s) 911
SmlI CTYRAG 2 cut(s) 660, 824
SmoI CTYRAG 2 cut(s) 660, 824
Sse9I AATT 9 cut(s) 79, 155, 256, 334, 606, 764, 841, 870, 939
SsiI CCGC 5 cut(s) 61, 120, 593, 867, 949
SspI AATATT 1 cut(s) 727
SspMI CTAG 3 cut(s) 191, 434, 635
StyD4I CCNGG 3 cut(s) 130, 131, 175
StyI CCWWGG 1 cut(s) 503
TaaI ACNGT 2 cut(s) 364, 971
TaiI ACGT 4 cut(s) 550, 741, 861, 885
TaqI TCGA 4 cut(s) 111, 199, 384, 526
TasI AATT 9 cut(s) 79, 155, 256, 334, 606, 764, 841, 870, 939
TauI GCSGC 3 cut(s) 63, 123, 595
TfiI GAWTC 4 cut(s) 68, 230, 322, 508
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TscAI CASTG 2 cut(s) 333, 915
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 5 cut(s) 430, 665, 862, 898, 926
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 6 cut(s) 24, 329, 360, 401, 618, 951
TspMI CCCGGG 1 cut(s) 131
TspRI CASTG 2 cut(s) 333, 915
Van91I CCANNNNNTGG 1 cut(s) 748
VpaK11BI GGWCC 3 cut(s) 551, 584, 703
XapI RAATTY 3 cut(s) 334, 764, 841
XmaI CCCGGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 885
XmnI GAANNNNTTC 2 cut(s) 205, 457
XspI CTAG 3 cut(s) 191, 434, 635
Zsp2I ATGCAT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.