Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr6

Orthogroup ID

OG0006113

Physical Location & Seq

Genomic Coordinates Forward (+)

2754611 .. 2757553

Gene Structure 2943 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2175 bp

ATGCTATATTGCTTTGAGCACTCGCAACTTCTTCCTCCTAAGTCGAAACCGTGTGCTTATGTCAATGGGTTATGTGTACGAAACTACGATACGAATCCGTTGGCCACGAGTCTCCCTGCTCTAGCAGCCACAACTGAAACCATCATTTCACTCTCAAGCTCAGCTTCTCCAACAATGGCGGCCATTGCTCCACCCTCCTCTTACTCCTTCTCAAAACCTTCCCCTTTCTCTTCCTCAGCCAAATCCCACCACCCCATTTCCAGATTCAACCCCCCCTTCTCTCCTCTTCCCCAAAAACCCTCTCCCCTCCGCCACCTCCGGATCTCCAGCTCCCTCTCCCCCAAAACCGATATCACAATCACAACCGCCGCCGCCACCACCACCACCACCACCACCAACCCTCTAGACCCAGAGGCCCCAATCTCCCGATTTGCCCCTGACGAGCCCAGAAAAGGCGCCGATGTCCTCGTTGAGGCGCTCGAGCGCCAAGGCGTCACCGACGTCTTTGCCTACCCTGGCGGCGCATCAATGGAGATCCACCAGGCTCTCACGCGCTCTTCTTCCATCCGCAACGTCCTGCCGCGCCATGAGCAAGGCGGGGTGTTCGCAGCTGAAGGCTACGCCCGCGCGTCGGGTCGTCCCGGCGTGTGCATTGCAACCTCCGGCCCCGGCGCAACGAACTTGGTTAGTGGCCTCGCCGACGCGCTCATGGACAGCATACCGGTGGTGGCAATCACCGGCCAAGTACCCCGGAGAATGATAGGCACTGACGCCTTCCAGGAGACCCCAATTGTTGAGGTAACAAGGTCAATCACTAAGCACAATTATCTTGTTCTTGATGTAGAGGACATTCCTAGGGTTGTTAGGGAGGCCTTTTTCTTAGCTACCTCTGGTCGACCTGGCCCGGTTTTGATTGATATACCCAAAGATGTGCAACAACAGCTTGTGGTTCCCAATTGGGATCAACCCATTAAGTTACCTGGGTACATGTCTAGGTTGCCCAAGTCCCCCAATGAGGGTCACTTGGAGCAGATTGTGAGGTTGGTGTCCGAGTCCAAGAAACCGGTTTTGTATGTTGGTGGAGGGTGTTTGAACTCCAGTGAGGAGTTGAGGCGGTTCGTGGAGCTCACGGGGATCCCCGTGGCTAGTACTTTGATGGGTCTCGGGGCATACCCGTGCAATGACGAAGAACTGTCACTCCAAATGCTTGGAATGCATGGCACTGTTTATGCTAATTATGCTGTGGATAAGTCTGATTTGCTGCTTGCATTTGGGGTGAGGTTTGATGACCGGGTTACCGGGAAGTTGGAGGCATTTGCTAGCCGGGCTAAGATTGTTCACATTGATATTGACTCGGCGGAGATTGGAAAGAATAAGCAGCCTCATGTGTCGGTTTGTGCTGATGTAAAATCGGCACTGGAAGGGTTGAATAGGATATTGGAGCAGAAAGAGAGCAAGGTTAAGCTTGATTTTTCAGCTTGGAGGGCGGAGCTGAAAGAGGAGAAAGTGAAGTTCCCATTGGGTTTTAAGACTTTTGGGGACTCCATTTCTCCTCAGAATGCCATTCAGGTTCTTGATGAGTTAACTGATGGGAATGCGATTATAAGCACTGGTGTTGGACAGCATCAAATGTGGGCAGCTCAGTTTTACAAGTACAAGCGGCCTCGGCAGTGGTTGACATCAGGGGGATTAGGAGCTATGGGCTTCGGATTGCCTGCTGCTATCGGGGCTGCTGTTGCAAACCCGGATTCTGTTGTCATTGACATTGACGGTGATGGAAGTTTCCTTATGAATGTCCAGGAATTGGCAACGATCAGTGTGGAGAAACTACCAGTCAAGATAATGCTGTTGAATAACCAGCATTTGGGTATGGTTGTGCAATGGGAGGACCGCTTCTACAAGGCAAACAGGGCTCATACTTACTTGGGGAACCCAGCAAATGAGTCGGAGATATTCCCAAATATGCTCAAGTTTGCAGATGCTTGTGGGATACCGGCTGCCCGTGTGACAAAGAAGGAAGATCTGAAAGCAGCAATTCAAAAAATGTTGGACACTCCTGGTCCATACTTGTTGGATGTAATAGTGCCCCATCAAGAGCATGTCCTGCCTATGATTCCGAGCGGCGGTGCTTTCAAAGATGTGATAACGGAGGGCGATGGGAGATCAAGTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003984 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006573 GO:0006807 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009081 GO:0009082 GO:0009099 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009987 GO:0016053 GO:0016740 GO:0016744 GO:0019752 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046394 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00290 ko00650 ko00660 ko00770 ko01100 ko01110 ko01130 ko01210 ko01230 map00290 map00650 map00660 map00770 map01100 map01110 map01130 map01210 map01230

Pfam Domains

Protein Families

TPP_enzyme_C TPP_enzyme_M TPP_enzyme_N

Protein Analysis

725

Amino Acids

78.33

Weight (kDa)

6.37

Isoelectric Point (pI)

42.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPP_enzyme_N	PF02776	151 - 261	1e-37	Thiamine pyrophosphate enzyme, N-terminal TPP binding domain
TPP_enzyme_M	PF00205	342 - 476	4.4e-44	Thiamine pyrophosphate enzyme, central domain
TPP_enzyme_C	PF02775	538 - 693	8.3e-51	Thiamine pyrophosphate enzyme, C-terminal TPP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006113)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48560
fragaria_vesca	FvH4_7g06770
malus_domestica	MD06G1028700.v1.1 MD10G1013700.v1.1
prunus_persica	Prupe.5G043100_v2.0.a1 Prupe.8G001300_v2.0.a1 Prupe.8G001300_v2.0.a1
pyrus_communis	pycom06g02280 pycom10g00840
rosa_chinensis	RchiOBHm_Chr7g0208791
rosa_laevigata	RLG00000003195 RLG00000015583
rosa_multiflora	Rmu_co8493263.1_g000001 Rmu_sc0000278.1_g000002 Rmu_sc0008663.1_g000001 Rmu_sc0018750.1_g000002
rosa_roxburghii	Rroxscaffold_3G00250260 Rroxscaffold_7G00217760
rosa_rugosa	Rorug05G0492000 Rorug07G0105800
rosa_samantha	Rh6AG001800 Rh7AG241000 Rh7BG235900 Rh7CG255600 Rh7DG247000
rosa_wichuraiana	Rw6G000240 Rw7G020440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1604
AatII	GACGTC	1 cut(s)	504
AccB1I	GGYRCC	1 cut(s)	455
AccB7I	CCANNNNNTGG	2 cut(s)	1804, 1864
AccBSI	CCGCTC	1 cut(s)	2121
AccI	GTMKAC	1 cut(s)	895
AccII	CGCG	5 cut(s)	553, 583, 627, 629, 704
AccIII	TCCGGA	1 cut(s)	318
AclWI	GGATC	5 cut(s)	329, 529, 969, 1129, 1142
AcoI	YGGCCR	3 cut(s)	102, 180, 739
AcuI	CTGAAG	1 cut(s)	633
AcyI	GRCGYC	4 cut(s)	456, 492, 501, 771
AfaI	GTAC	5 cut(s)	78, 747, 986, 1150, 1655
AfiI	CCNNNNNNNGG	8 cut(s)	452, 472, 631, 1015, 1016, 1804, 1864, 2123
AflIII	ACRYGT	1 cut(s)	987
AgeI	ACCGGT	2 cut(s)	721, 1063
AgsI	TTSAA	6 cut(s)	268, 1093, 1429, 1852, 2039, 2134
AjnI	CCWGG	7 cut(s)	514, 540, 777, 898, 979, 1797, 2056
AjuI	GAANNNNNNNTTGG	4 cut(s)	1421, 1453, 1502, 1534
AloI	GAACNNNNNNTCC	2 cut(s)	1182, 1214
Alw21I	GWGCWC	2 cut(s)	21, 1128
Alw26I	GTCTC	3 cut(s)	116, 776, 1166
AlwI	GGATC	5 cut(s)	329, 529, 969, 1129, 1142
Ama87I	CYCGRG	2 cut(s)	479, 1163
Aor13HI	TCCGGA	1 cut(s)	318
AoxI	GGCC	9 cut(s)	102, 180, 414, 664, 691, 739, 870, 901, 1661
ApeKI	GCWGC	9 cut(s)	125, 608, 1261, 1378, 1637, 1718, 1730, 1997, 2030
AsiGI	ACCGGT	2 cut(s)	721, 1063
AspA2I	CCTAGG	1 cut(s)	854
AspLEI	GCGC	9 cut(s)	458, 478, 486, 524, 555, 585, 629, 674, 706
AspS9I	GGNCC	5 cut(s)	415, 665, 902, 1888, 2060
AsuC2I	CCSGG	8 cut(s)	642, 669, 751, 905, 1292, 1300, 1325, 1745
AsuHPI	GGTGA	4 cut(s)	487, 727, 1288, 1784
AsuNHI	GCTAGC	1 cut(s)	1319
AvaI	CYCGRG	2 cut(s)	479, 1163
AvaII	GGWCC	2 cut(s)	1888, 2060
AvrII	CCTAGG	1 cut(s)	854
BaeGI	GKGCMC	1 cut(s)	2088
BalI	TGGCCA	1 cut(s)	104
BamHI	GGATCC	1 cut(s)	1134
BanI	GGYRCC	1 cut(s)	455
BanII	GRGCYC	3 cut(s)	447, 1128, 1915
BauI	CACGAG	1 cut(s)	106
Bbv12I	GWGCWC	2 cut(s)	21, 1128
BbvCI	CCTCAGC	1 cut(s)	235
BbvI	GCAGC	9 cut(s)	137, 620, 1248, 1390, 1649, 1705, 1717, 1984, 2042
BccI	CCATC	7 cut(s)	149, 572, 1150, 1583, 1769, 2097, 2150
BcgI	CGANNNNNNTGC	4 cut(s)	488, 522, 1926, 1960
BciT130I	CCWGG	7 cut(s)	516, 542, 779, 900, 981, 1799, 2058
BciVI	GTATCC	1 cut(s)	1983
BcnI	CCSGG	8 cut(s)	642, 669, 751, 905, 1292, 1300, 1325, 1745
BcoDI	GTCTC	3 cut(s)	116, 776, 1166
BfaI	CTAG	6 cut(s)	122, 404, 855, 993, 1146, 1320
BfoI	RGCGCY	3 cut(s)	459, 479, 487
BfuI	GTATCC	1 cut(s)	1983
BglII	AGATCT	1 cut(s)	2020
BlnI	CCTAGG	1 cut(s)	854
BlpI	GCTNAGC	1 cut(s)	160
BmcAI	AGTACT	1 cut(s)	1150
Bme18I	GGWCC	2 cut(s)	1888, 2060
BmeT110I	CYCGRG	2 cut(s)	479, 1163
BmgT120I	GGNCC	5 cut(s)	415, 665, 902, 1888, 2060
BmiI	GGNNCC	6 cut(s)	417, 457, 667, 951, 1136, 1931
BmsI	GCATC	3 cut(s)	533, 1633, 1969
BmtI	GCTAGC	1 cut(s)	1323
BpmI	CTGGAG	2 cut(s)	310, 1081
Bpu10I	CCTNAGC	1 cut(s)	235
Bpu1102I	GCTNAGC	1 cut(s)	160
BpuEI	CTTGAG	2 cut(s)	139, 1952
BpuMI	CCSGG	8 cut(s)	642, 669, 751, 905, 1292, 1300, 1325, 1745
BsaBI	GATNNNNATC	1 cut(s)	93
BsaHI	GRCGYC	4 cut(s)	456, 492, 501, 771
BsaI	GGTCTC	2 cut(s)	776, 1166
BsaJI	CCNNGG	8 cut(s)	487, 514, 667, 749, 854, 980, 1140, 1664
BsaWI	WCCGGW	3 cut(s)	318, 721, 1063
BsaXI	ACNNNNNCTCC	3 cut(s)	1182, 1212, 2152
Bsc4I	CCNNNNNNNGG	8 cut(s)	452, 472, 631, 1015, 1016, 1804, 1864, 2123
Bse118I	RCCGGY	4 cut(s)	721, 737, 1063, 1993
Bse1I	ACTGG	4 cut(s)	1098, 1422, 1615, 1832
Bse3DI	GCAATG	4 cut(s)	183, 651, 1186, 1886
Bse8I	GATNNNNATC	1 cut(s)	93
BseAI	TCCGGA	1 cut(s)	318
BseBI	CCWGG	7 cut(s)	516, 542, 779, 900, 981, 1799, 2058
BseDI	CCNNGG	8 cut(s)	487, 514, 667, 749, 854, 980, 1140, 1664
BseGI	GGATG	2 cut(s)	564, 2080
BseJI	GATNNNNATC	1 cut(s)	93
BseLI	CCNNNNNNNGG	8 cut(s)	452, 472, 631, 1015, 1016, 1804, 1864, 2123
BseMI	GCAATG	4 cut(s)	183, 651, 1186, 1886
BseMII	CTCAG	4 cut(s)	174, 249, 1568, 1655
BseNI	ACTGG	4 cut(s)	1098, 1422, 1615, 1832
BseRI	GAGGAG	5 cut(s)	187, 273, 1118, 1514, 1542
BseSI	GKGCMC	1 cut(s)	2088
BseXI	GCAGC	9 cut(s)	137, 620, 1248, 1390, 1649, 1705, 1717, 1984, 2042
BseYI	CCCAGC	1 cut(s)	1933
Bsh1236I	CGCG	5 cut(s)	553, 583, 627, 629, 704
BshFI	GGCC	9 cut(s)	104, 182, 416, 666, 693, 741, 872, 903, 1663
BshNI	GGYRCC	1 cut(s)	455
BshTI	ACCGGT	2 cut(s)	721, 1063
BsiHKAI	GWGCWC	2 cut(s)	21, 1128
BsiHKCI	CYCGRG	2 cut(s)	479, 1163
BslFI	GGGAC	3 cut(s)	624, 991, 1553
BslI	CCNNNNNNNGG	8 cut(s)	452, 472, 631, 1015, 1016, 1804, 1864, 2123
BsmAI	GTCTC	3 cut(s)	116, 776, 1166
BsmFI	GGGAC	3 cut(s)	624, 991, 1553
BsmI	GAATGC	3 cut(s)	1218, 1564, 1600
BsnI	GGCC	9 cut(s)	104, 182, 416, 666, 693, 741, 872, 903, 1663
Bso31I	GGTCTC	2 cut(s)	776, 1166
BsoBI	CYCGRG	2 cut(s)	479, 1163
Bsp1286I	GDGCHC	5 cut(s)	21, 447, 1128, 1915, 2088
Bsp13I	TCCGGA	1 cut(s)	318
Bsp143I	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
Bsp1720I	GCTNAGC	1 cut(s)	160
BspANI	GGCC	9 cut(s)	104, 182, 416, 666, 693, 741, 872, 903, 1663
BspCNI	CTCAG	4 cut(s)	173, 248, 1567, 1654
BspEI	TCCGGA	1 cut(s)	318
BspFNI	CGCG	5 cut(s)	553, 583, 627, 629, 704
BspLI	GGNNCC	6 cut(s)	417, 457, 667, 951, 1136, 1931
BspOI	GCTAGC	1 cut(s)	1323
BspPI	GGATC	5 cut(s)	329, 529, 969, 1129, 1142
BspQI	GCTCTTC	1 cut(s)	562
BspT107I	GGYRCC	1 cut(s)	455
BspTNI	GGTCTC	2 cut(s)	776, 1166
BsrBI	CCGCTC	1 cut(s)	2121
BsrDI	GCAATG	4 cut(s)	183, 651, 1186, 1886
BsrFI	RCCGGY	4 cut(s)	721, 737, 1063, 1993
BsrI	ACTGG	4 cut(s)	1098, 1422, 1615, 1832
BssAI	RCCGGY	4 cut(s)	721, 737, 1063, 1993
BssECI	CCNNGG	8 cut(s)	487, 514, 667, 749, 854, 980, 1140, 1664
BssMI	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
BssNI	GRCGYC	4 cut(s)	456, 492, 501, 771
BssSI	CACGAG	1 cut(s)	106
BssT1I	CCWWGG	2 cut(s)	487, 854
Bst2BI	CACGAG	1 cut(s)	106
Bst2UI	CCWGG	7 cut(s)	516, 542, 779, 900, 981, 1799, 2058
Bst4CI	ACNGT	4 cut(s)	51, 1194, 1225, 1772
Bst6I	CTCTTC	3 cut(s)	235, 291, 562
BstACI	GRCGYC	4 cut(s)	456, 492, 501, 771
BstAPI	GCANNNNNTGC	1 cut(s)	2104
BstC8I	GCNNGC	4 cut(s)	625, 1266, 1321, 1716
BstDEI	CTNAG	8 cut(s)	39, 160, 235, 816, 880, 1329, 1554, 1641
BstDSI	CCRYGG	1 cut(s)	1140
BstEII	GGTNACC	1 cut(s)	1294
BstENI	CCTNNNNNAGG	1 cut(s)	470
BstF5I	GGATG	2 cut(s)	564, 2080
BstFNI	CGCG	5 cut(s)	553, 583, 627, 629, 704
BstH2I	RGCGCY	3 cut(s)	459, 479, 487
BstHHI	GCGC	9 cut(s)	458, 478, 486, 524, 555, 585, 629, 674, 706
BstKTI	GATC	7 cut(s)	324, 537, 964, 1137, 1815, 2023, 2165
BstMAI	GTCTC	3 cut(s)	116, 776, 1166
BstMBI	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
BstNI	CCWGG	7 cut(s)	516, 542, 779, 900, 981, 1799, 2058
BstNSI	RCATGY	2 cut(s)	991, 2102
BstPI	GGTNACC	1 cut(s)	1294
BstSLI	GKGCMC	1 cut(s)	2088
BstUI	CGCG	5 cut(s)	553, 583, 627, 629, 704
BstV1I	GCAGC	9 cut(s)	137, 620, 1248, 1390, 1649, 1705, 1717, 1984, 2042
BstX2I	RGATCY	4 cut(s)	321, 534, 1134, 2020
BstXI	CCANNNNNNTGG	1 cut(s)	1208
BstYI	RGATCY	4 cut(s)	321, 534, 1134, 2020
BsuI	GTATCC	1 cut(s)	1983
BsuRI	GGCC	9 cut(s)	104, 182, 416, 666, 693, 741, 872, 903, 1663
BtgI	CCRYGG	1 cut(s)	1140
BtgZI	GCGATG	1 cut(s)	2169
BtsCI	GGATG	2 cut(s)	564, 2080
BtsI	GCAGTG	1 cut(s)	1676
BtsIMutI	CAGTG	7 cut(s)	765, 1105, 1221, 1415, 1608, 1676, 1822
Cac8I	GCNNGC	4 cut(s)	625, 1266, 1321, 1716
CfoI	GCGC	9 cut(s)	458, 478, 486, 524, 555, 585, 629, 674, 706
Cfr10I	RCCGGY	4 cut(s)	721, 737, 1063, 1993
Cfr13I	GGNCC	5 cut(s)	415, 665, 902, 1888, 2060
CseI	GACGC	4 cut(s)	481, 618, 710, 779
Csp6I	GTAC	5 cut(s)	77, 746, 985, 1149, 1654
CspAI	ACCGGT	2 cut(s)	721, 1063
CviAII	CATG	6 cut(s)	587, 709, 988, 1217, 1385, 2099
CviQI	GTAC	5 cut(s)	77, 746, 985, 1149, 1654
DdeI	CTNAG	8 cut(s)	39, 160, 235, 816, 880, 1329, 1554, 1641
DinI	GGCGCC	1 cut(s)	457
DpnI	GATC	7 cut(s)	323, 536, 963, 1136, 1814, 2022, 2164
DpnII	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
EaeI	YGGCCR	3 cut(s)	102, 180, 739
Eam1104I	CTCTTC	3 cut(s)	235, 291, 562
EarI	CTCTTC	3 cut(s)	235, 291, 562
EciI	GGCGGA	3 cut(s)	299, 1373, 1502
Ecl136II	GAGCTC	1 cut(s)	1126
Eco130I	CCWWGG	2 cut(s)	487, 854
Eco147I	AGGCCT	1 cut(s)	872
Eco24I	GRGCYC	3 cut(s)	447, 1128, 1915
Eco31I	GGTCTC	2 cut(s)	776, 1166
Eco32I	GATATC	1 cut(s)	352
Eco47I	GGWCC	2 cut(s)	1888, 2060
Eco53kI	GAGCTC	1 cut(s)	1126
Eco57I	CTGAAG	1 cut(s)	633
Eco88I	CYCGRG	2 cut(s)	479, 1163
Eco91I	GGTNACC	1 cut(s)	1294
EcoICRI	GAGCTC	1 cut(s)	1126
EcoNI	CCTNNNNNAGG	1 cut(s)	470
EcoO109I	RGGNCCY	1 cut(s)	415
EcoO65I	GGTNACC	1 cut(s)	1294
EcoRII	CCWGG	7 cut(s)	514, 540, 777, 898, 979, 1797, 2056
EcoRV	GATATC	1 cut(s)	352
EcoT14I	CCWWGG	2 cut(s)	487, 854
EcoT22I	ATGCAT	1 cut(s)	1218
EcoT38I	GRGCYC	3 cut(s)	447, 1128, 1915
EgeI	GGCGCC	1 cut(s)	457
EheI	GGCGCC	1 cut(s)	457
ErhI	CCWWGG	2 cut(s)	487, 854
FaeI	CATG	6 cut(s)	590, 712, 991, 1220, 1388, 2102
FalI	AAGNNNNNCTT	2 cut(s)	148, 180
FaqI	GGGAC	3 cut(s)	624, 991, 1553
FatI	CATG	6 cut(s)	586, 708, 987, 1216, 1384, 2098
FauI	CCCGC	2 cut(s)	590, 632
FblI	GTMKAC	1 cut(s)	895
FokI	GGATG	2 cut(s)	551, 2087
FriOI	GRGCYC	3 cut(s)	447, 1128, 1915
FspBI	CTAG	6 cut(s)	122, 404, 855, 993, 1146, 1320
GlaI	GCGC	9 cut(s)	457, 477, 485, 523, 554, 584, 628, 673, 705
GsaI	CCCAGC	1 cut(s)	1937
GsuI	CTGGAG	2 cut(s)	310, 1081
HaeII	RGCGCY	3 cut(s)	459, 479, 487
HaeIII	GGCC	9 cut(s)	104, 182, 416, 666, 693, 741, 872, 903, 1663
HgaI	GACGC	4 cut(s)	481, 618, 710, 779
HhaI	GCGC	9 cut(s)	458, 478, 486, 524, 555, 585, 629, 674, 706
Hin1I	GRCGYC	4 cut(s)	456, 492, 501, 771
Hin1II	CATG	6 cut(s)	590, 712, 991, 1220, 1388, 2102
Hin6I	GCGC	9 cut(s)	456, 476, 484, 522, 553, 583, 627, 672, 704
HinP1I	GCGC	9 cut(s)	456, 476, 484, 522, 553, 583, 627, 672, 704
HincII	GTYRAC	3 cut(s)	896, 1584, 1677
HindII	GTYRAC	3 cut(s)	896, 1584, 1677
HindIII	AAGCTT	1 cut(s)	1463
HinfI	GANTC	9 cut(s)	94, 109, 264, 1052, 1352, 1541, 1748, 1943, 2113
HpaI	GTTAAC	1 cut(s)	1584
HphI	GGTGA	4 cut(s)	487, 727, 1288, 1784
Hpy166II	GTNNAC	5 cut(s)	77, 896, 1339, 1584, 1677
Hpy188I	TCNGA	7 cut(s)	1051, 1255, 1557, 1709, 1948, 2025, 2118
Hpy188III	TCNNGA	8 cut(s)	261, 319, 404, 426, 836, 1574, 1837, 2093
Hpy8I	GTNNAC	5 cut(s)	77, 896, 1339, 1584, 1677
Hpy99I	CGWCG	3 cut(s)	503, 634, 704
HpyAV	CCTTC	7 cut(s)	217, 228, 286, 608, 784, 1415, 2008
HpyCH4III	ACNGT	4 cut(s)	51, 1194, 1225, 1772
HpyCH4IV	ACGT	2 cut(s)	501, 573
HpyCH4V	TGCA	9 cut(s)	651, 656, 934, 1179, 1216, 1268, 1739, 1879, 1976
HpyF3I	CTNAG	8 cut(s)	39, 160, 235, 816, 880, 1329, 1554, 1641
HpySE526I	ACGT	2 cut(s)	501, 573
Hsp92I	GRCGYC	4 cut(s)	456, 492, 501, 771
Hsp92II	CATG	6 cut(s)	590, 712, 991, 1220, 1388, 2102
HspAI	GCGC	9 cut(s)	456, 476, 484, 522, 553, 583, 627, 672, 704
KasI	GGCGCC	1 cut(s)	455
Kpn2I	TCCGGA	1 cut(s)	318
KspAI	GTTAAC	1 cut(s)	1584
Kzo9I	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
LguI	GCTCTTC	1 cut(s)	562
LmnI	GCTCC	7 cut(s)	193, 335, 1027, 1123, 1441, 1489, 1694
Lsp1109I	GCAGC	9 cut(s)	137, 620, 1248, 1390, 1649, 1705, 1717, 1984, 2042
LweI	GCATC	3 cut(s)	533, 1633, 1969
MaeI	CTAG	6 cut(s)	122, 404, 855, 993, 1146, 1320
MaeII	ACGT	2 cut(s)	501, 573
MaeIII	GTNAC	7 cut(s)	493, 799, 975, 1019, 1194, 1294, 2005
MalI	GATC	7 cut(s)	323, 536, 963, 1136, 1814, 2022, 2164
MbiI	CCGCTC	1 cut(s)	2121
MboI	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
MboII	GAAGA	7 cut(s)	23, 222, 278, 549, 552, 1199, 2030
MfeI	CAATTG	2 cut(s)	789, 955
MflI	RGATCY	4 cut(s)	321, 534, 1134, 2020
MhlI	GDGCHC	5 cut(s)	21, 447, 1128, 1915, 2088
MlsI	TGGCCA	1 cut(s)	104
MluCI	AATT	6 cut(s)	789, 823, 955, 1234, 1802, 2034
MluNI	TGGCCA	1 cut(s)	104
Mly113I	GGCGCC	1 cut(s)	456
MlyI	GAGTC	5 cut(s)	118, 1061, 1346, 1535, 1952
MmeI	TCCRAC	6 cut(s)	194, 1287, 1597, 1926, 2028, 2052
Mox20I	TGGCCA	1 cut(s)	104
Mph1103I	ATGCAT	1 cut(s)	1218
MroI	TCCGGA	1 cut(s)	318
MscI	TGGCCA	1 cut(s)	104
MseI	TTAA	4 cut(s)	972, 1461, 1527, 1583
MslI	CAYNNNNRTG	2 cut(s)	722, 1174
Msp20I	TGGCCA	1 cut(s)	104
MspA1I	CMGCKG	1 cut(s)	611
MunI	CAATTG	2 cut(s)	789, 955
Mva1269I	GAATGC	3 cut(s)	1218, 1564, 1600
MvaI	CCWGG	7 cut(s)	516, 542, 779, 900, 981, 1799, 2058
MvnI	CGCG	5 cut(s)	553, 583, 627, 629, 704
NarI	GGCGCC	1 cut(s)	456
NciI	CCSGG	8 cut(s)	642, 669, 751, 905, 1292, 1300, 1325, 1745
NdeII	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
NheI	GCTAGC	1 cut(s)	1319
NlaIII	CATG	6 cut(s)	590, 712, 991, 1220, 1388, 2102
NlaIV	GGNNCC	6 cut(s)	417, 457, 667, 951, 1136, 1931
NmeAIII	GCCGAG	2 cut(s)	1334, 1645
NmuCI	GTSAC	4 cut(s)	493, 1019, 1194, 2005
NsiI	ATGCAT	1 cut(s)	1218
NspI	RCATGY	2 cut(s)	991, 2102
PaeR7I	CTCGAG	1 cut(s)	479
PceI	AGGCCT	1 cut(s)	872
PciI	ACATGT	1 cut(s)	987
PciSI	GCTCTTC	1 cut(s)	562
PctI	GAATGC	3 cut(s)	1218, 1564, 1600
PfeI	GAWTC	4 cut(s)	94, 264, 1748, 2113
PflMI	CCANNNNNTGG	2 cut(s)	1804, 1864
PfoI	TCCNGGA	2 cut(s)	777, 1797
PinAI	ACCGGT	2 cut(s)	721, 1063
PleI	GAGTC	5 cut(s)	117, 1060, 1346, 1535, 1951
PluTI	GGCGCC	1 cut(s)	459
PpsI	GAGTC	5 cut(s)	117, 1060, 1346, 1535, 1951
PscI	ACATGT	1 cut(s)	987
PsiI	TTATAA	1 cut(s)	1604
Psp124BI	GAGCTC	1 cut(s)	1128
Psp6I	CCWGG	7 cut(s)	514, 540, 777, 898, 979, 1797, 2056
PspEI	GGTNACC	1 cut(s)	1294
PspFI	CCCAGC	1 cut(s)	1933
PspGI	CCWGG	7 cut(s)	514, 540, 777, 898, 979, 1797, 2056
PspN4I	GGNNCC	6 cut(s)	417, 457, 667, 951, 1136, 1931
PspPI	GGNCC	5 cut(s)	415, 665, 902, 1888, 2060
PspXI	VCTCGAGB	1 cut(s)	479
PsuI	RGATCY	4 cut(s)	321, 534, 1134, 2020
PvuII	CAGCTG	1 cut(s)	611
RsaI	GTAC	5 cut(s)	78, 747, 986, 1150, 1655
RsaNI	GTAC	5 cut(s)	77, 746, 985, 1149, 1654
RseI	CAYNNNNRTG	2 cut(s)	722, 1174
SacI	GAGCTC	1 cut(s)	1128
SalI	GTCGAC	1 cut(s)	894
SapI	GCTCTTC	1 cut(s)	562
SaqAI	TTAA	4 cut(s)	972, 1461, 1527, 1583
Sau3AI	GATC	7 cut(s)	321, 534, 961, 1134, 1812, 2020, 2162
Sau96I	GGNCC	5 cut(s)	415, 665, 902, 1888, 2060
ScaI	AGTACT	1 cut(s)	1150
SchI	GAGTC	5 cut(s)	118, 1061, 1346, 1535, 1952
SduI	GDGCHC	5 cut(s)	21, 447, 1128, 1915, 2088
SfaNI	GCATC	3 cut(s)	533, 1633, 1969
SfoI	GGCGCC	1 cut(s)	457
Sfr274I	CTCGAG	1 cut(s)	479
SinI	GGWCC	2 cut(s)	1888, 2060
SlaI	CTCGAG	1 cut(s)	479
SmiMI	CAYNNNNRTG	2 cut(s)	722, 1174
SmlI	CTYRAG	3 cut(s)	154, 479, 1967
SmoI	CTYRAG	3 cut(s)	154, 479, 1967
Sse9I	AATT	6 cut(s)	789, 823, 955, 1234, 1802, 2034
SseBI	AGGCCT	1 cut(s)	872
SspDI	GGCGCC	1 cut(s)	455
SspMI	CTAG	6 cut(s)	122, 404, 855, 993, 1146, 1320
SstI	GAGCTC	1 cut(s)	1128
StuI	AGGCCT	1 cut(s)	872
StyI	CCWWGG	2 cut(s)	487, 854
TaaI	ACNGT	4 cut(s)	51, 1194, 1225, 1772
TaiI	ACGT	2 cut(s)	504, 576
TaqI	TCGA	3 cut(s)	44, 480, 895
TasI	AATT	6 cut(s)	789, 823, 955, 1234, 1802, 2034
TatI	WGTACW	2 cut(s)	1148, 1653
TauI	GCSGC	7 cut(s)	182, 371, 374, 522, 583, 1663, 2124
TfiI	GAWTC	4 cut(s)	94, 264, 1748, 2113
Tru1I	TTAA	4 cut(s)	972, 1461, 1527, 1583
Tru9I	TTAA	4 cut(s)	972, 1461, 1527, 1583
TscAI	CASTG	7 cut(s)	772, 1105, 1228, 1422, 1615, 1676, 1822
TseFI	GTSAC	4 cut(s)	493, 1019, 1194, 2005
TseI	GCWGC	9 cut(s)	125, 608, 1261, 1378, 1637, 1718, 1730, 1997, 2030
Tsp45I	GTSAC	4 cut(s)	493, 1019, 1194, 2005
TspDTI	ATGAA	1 cut(s)	1805
TspGWI	ACGGA	2 cut(s)	87, 2162
TspRI	CASTG	7 cut(s)	772, 1105, 1228, 1422, 1615, 1676, 1822
Van91I	CCANNNNNTGG	2 cut(s)	1804, 1864
VpaK11BI	GGWCC	2 cut(s)	1888, 2060
XagI	CCTNNNNNAGG	1 cut(s)	470
XbaI	TCTAGA	1 cut(s)	403
XceI	RCATGY	2 cut(s)	991, 2102
XhoI	CTCGAG	1 cut(s)	479
XmaJI	CCTAGG	1 cut(s)	854
XmiI	GTMKAC	1 cut(s)	895
XspI	CTAG	6 cut(s)	122, 404, 855, 993, 1146, 1320
ZraI	GACGTC	1 cut(s)	502
ZrmI	AGTACT	1 cut(s)	1150
Zsp2I	ATGCAT	1 cut(s)	1218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.