pycom06g05780
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0008211
Physical Location & Seq
Reverse (-)
8712146 .. 8713814
1669 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1149 bp
ATGGATACACTTTCACATGTACCCCCAGGCTTTCGATTCCATCCCACAGATGAAGAACTTGTTGATTATTATCTTAGAAAGAAGACTGCTTCGAAACCAATTGATTTTGATGTCGTCAGAGATGTTGATCTCTATAAGATTGAGCCATGGGATCTTGAAGATTTGTGCAAAATAGGAAGTGGTGATCAGAATGAATGGTACTTCTTTAACCATAAAGATAAGAAGTACCCTACCGGACACGATCGATCAAATAGGGCAACAAAAGCTGGGTTTTGGAAAGCTACAGGAAGAGATAAGGCTATTTACAGAGGAAAATTCCTTGTTGGCATGAGAAAGACTTTAGTCTTCTACAAGGGCCGTGCTCCAAATGGACAGAAGTCAGATTGGATCATGCATGAATATCGACTAGAAACTAGTGAAAATGGAACTCCTCAGGCAAAAGGATGGGTTGTGTGTAGAGTGTTCACGAAAAAATTGCCAACAGTGAGGAGAATGGGAGATTACGAGTCACCAAATTGCTATGACCAAGTCCCATTCATGCAACAACTTGATAACTCTCCAAGAACATCAATTTCCCACACTTACGTATCATCACAATATCAGCAGCAGCTACACTATTCACAATGCAAGCCAGAGCTTGATTTGCAGTACAACATGCCACATCATCATCATGACTCTTTTCTCCAGCTCCCTCAATTGGAAAGCCCCAAAGTTCCTCATCAGCCAGCTACCCCATATGGTAATTATGTTATGCAGTCTTCCACACTCACACAAGAGGAGCAATTGCATTACTTCTGCCCGCAAAATCCTTTCAACTCATCTGCGTCGTCATCAGGACATATGCATTACAACAACACTGATTATCTACAAGTTGTTGATCAAGTGAAGGATTGGCGAGCCCTTGACAAGTATGTTGCATCACAGCTCAGCCATGACCAGCAAGATGCTTCCAAGGAAGTCACAAATTACTCCAATGCACAAGAAACATTTCATGTGGCTGAACACATTAATATGCTTGCCAATGAAGCTTCCAGAAGGCCAGACTATAATAATAATGGACAGGAGTACTACGCCTCAACATCAACCTCTAGTTGCCAAATTGATCGATCTGGAAATGATCAAGAACATGAATATTACAGTAACTGTTAG

Protein Analysis

383

Amino Acids

44.51

Weight (kDa)

6.25

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 8 - 136 2.5e-46 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 800
AclWI GGATC 2 cut(s) 159, 395
AcsI RAATTY 1 cut(s) 314
AfaI GTAC 5 cut(s) 21, 200, 227, 650, 1067
AfiI CCNNNNNNNGG 1 cut(s) 697
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 2 cut(s) 158, 814
AhlI ACTAGT 1 cut(s) 413
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 8 cut(s) 266, 281, 610, 637, 688, 728, 925, 1028
AluI AGCT 8 cut(s) 266, 281, 610, 637, 688, 728, 925, 1028
Alw21I GWGCWC 1 cut(s) 364
AlwI GGATC 2 cut(s) 159, 395
AlwNI CAGNNNCTG 1 cut(s) 1143
AoxI GGCC 2 cut(s) 355, 1037
ApeKI GCWGC 2 cut(s) 604, 607
ApoI RAATTY 1 cut(s) 314
AseI ATTAAT 1 cut(s) 1008
Asp700I GAANNNNTTC 1 cut(s) 987
AspS9I GGNCC 1 cut(s) 355
AsuHPI GGTGA 2 cut(s) 194, 501
AsuII TTCGAA 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 432
BaeI ACNNNNGTAYC 2 cut(s) 570, 603
BanII GRGCYC 1 cut(s) 901
BbsI GAAGAC 3 cut(s) 89, 337, 750
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 2 cut(s) 616, 619
BccI CCATC 2 cut(s) 48, 438
BceAI ACGGC 1 cut(s) 342
BcgI CGANNNNNNTGC 4 cut(s) 383, 417, 457, 491
BciT130I CCWGG 1 cut(s) 27
BclI TGATCA 3 cut(s) 184, 877, 1117
BcuI ACTAGT 1 cut(s) 413
BfaI CTAG 3 cut(s) 407, 414, 1089
BfmI CTRYAG 1 cut(s) 282
BisI GCNGC 2 cut(s) 605, 608
BlpI GCTNAGC 1 cut(s) 926
BlsI GCNGC 2 cut(s) 606, 609
BmcAI AGTACT 1 cut(s) 1067
Bme1390I CCNGG 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 355
BmrFI CCNGG 1 cut(s) 27
BmsI GCATC 2 cut(s) 926, 934
BoxI GACNNNNGTC 2 cut(s) 341, 376
BpiI GAAGAC 3 cut(s) 89, 337, 750
BpmI CTGGAG 1 cut(s) 668
Bpu1102I GCTNAGC 1 cut(s) 926
Bpu14I TTCGAA 1 cut(s) 92
Bsa29I ATCGAT 2 cut(s) 244, 1105
BsaAI YACGTR 1 cut(s) 586
BsaBI GATNNNNATC 2 cut(s) 69, 126
BsaJI CCNNGG 3 cut(s) 25, 146, 951
BsaWI WCCGGW 1 cut(s) 233
Bsc4I CCNNNNNNNGG 1 cut(s) 697
Bse21I CCTNAGG 1 cut(s) 432
Bse8I GATNNNNATC 2 cut(s) 69, 126
BseBI CCWGG 1 cut(s) 27
BseCI ATCGAT 2 cut(s) 244, 1105
BseDI CCNNGG 3 cut(s) 25, 146, 951
BseGI GGATG 2 cut(s) 40, 449
BseJI GATNNNNATC 2 cut(s) 69, 126
BseLI CCNNNNNNNGG 1 cut(s) 697
BseMII CTCAG 2 cut(s) 446, 940
BseRI GAGGAG 3 cut(s) 420, 502, 791
BseXI GCAGC 2 cut(s) 616, 619
BseYI CCCAGC 1 cut(s) 266
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 2 cut(s) 357, 1039
BshVI ATCGAT 2 cut(s) 244, 1105
BsiEI CGRYCG 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 364
BsiSI CCGG 1 cut(s) 234
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 1 cut(s) 697
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 2 cut(s) 357, 1039
Bsp119I TTCGAA 1 cut(s) 92
Bsp1286I GDGCHC 2 cut(s) 364, 901
Bsp1720I GCTNAGC 1 cut(s) 926
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 1 cut(s) 800
BspANI GGCC 2 cut(s) 357, 1039
BspCNI CTCAG 2 cut(s) 445, 939
BspDI ATCGAT 2 cut(s) 244, 1105
BspHI TCATGA 1 cut(s) 670
BspPI GGATC 2 cut(s) 159, 395
BspT104I TTCGAA 1 cut(s) 92
BssECI CCNNGG 3 cut(s) 25, 146, 951
BssT1I CCWWGG 2 cut(s) 146, 951
Bst2UI CCWGG 1 cut(s) 27
Bst4CI ACNGT 3 cut(s) 484, 1139, 1145
Bst6I CTCTTC 1 cut(s) 283
BstBAI YACGTR 1 cut(s) 586
BstBI TTCGAA 1 cut(s) 92
BstC8I GCNNGC 5 cut(s) 629, 726, 800, 897, 1017
BstDEI CTNAG 3 cut(s) 74, 432, 926
BstDSI CCRYGG 1 cut(s) 146
BstF5I GGATG 2 cut(s) 40, 449
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 263, 643, 1025
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 2 cut(s) 20, 658
BstPAI GACNNNNGTC 2 cut(s) 341, 376
BstSCI CCNGG 1 cut(s) 25
BstSFI CTRYAG 1 cut(s) 282
BstSNI TACGTA 1 cut(s) 586
BstV1I GCAGC 2 cut(s) 616, 619
BstV2I GAAGAC 3 cut(s) 89, 337, 750
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
Bsu15I ATCGAT 2 cut(s) 244, 1105
Bsu36I CCTNAGG 1 cut(s) 432
BsuRI GGCC 2 cut(s) 357, 1039
BsuTUI ATCGAT 2 cut(s) 244, 1105
BtgI CCRYGG 1 cut(s) 146
BtsCI GGATG 2 cut(s) 40, 449
BtsIMutI CAGTG 2 cut(s) 489, 855
Cac8I GCNNGC 5 cut(s) 629, 726, 800, 897, 1017
CaiI CAGNNNCTG 1 cut(s) 1143
CciI TCATGA 1 cut(s) 670
Cfr13I GGNCC 1 cut(s) 355
ClaI ATCGAT 2 cut(s) 244, 1105
CseI GACGC 1 cut(s) 813
Csp6I GTAC 5 cut(s) 20, 199, 226, 649, 1066
CviQI GTAC 5 cut(s) 20, 199, 226, 649, 1066
DdeI CTNAG 3 cut(s) 74, 432, 926
Eam1104I CTCTTC 1 cut(s) 283
EarI CTCTTC 1 cut(s) 283
Eco105I TACGTA 1 cut(s) 586
Eco130I CCWWGG 2 cut(s) 146, 951
Eco24I GRGCYC 1 cut(s) 901
Eco81I CCTNAGG 1 cut(s) 432
EcoRII CCWGG 1 cut(s) 25
EcoT14I CCWWGG 2 cut(s) 146, 951
EcoT22I ATGCAT 2 cut(s) 396, 846
EcoT38I GRGCYC 1 cut(s) 901
ErhI CCWWGG 2 cut(s) 146, 951
FaqI GGGAC 1 cut(s) 515
FauI CCCGC 1 cut(s) 807
FauNDI CATATG 2 cut(s) 736, 840
FbaI TGATCA 3 cut(s) 184, 877, 1117
Fnu4HI GCNGC 2 cut(s) 605, 608
FokI GGATG 2 cut(s) 27, 456
FriOI GRGCYC 1 cut(s) 901
Fsp4HI GCNGC 2 cut(s) 605, 608
FspBI CTAG 3 cut(s) 407, 414, 1089
GluI GCNGC 2 cut(s) 605, 608
GsaI CCCAGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 668
HaeIII GGCC 2 cut(s) 357, 1039
HapII CCGG 1 cut(s) 234
HgaI GACGC 1 cut(s) 813
HindIII AAGCTT 1 cut(s) 1026
HinfI GANTC 3 cut(s) 36, 506, 674
HpaII CCGG 1 cut(s) 234
HphI GGTGA 2 cut(s) 194, 501
Hpy166II GTNNAC 1 cut(s) 465
Hpy188I TCNGA 3 cut(s) 119, 189, 382
Hpy188III TCNNGA 7 cut(s) 155, 466, 671, 834, 1032, 1110, 1121
Hpy8I GTNNAC 1 cut(s) 465
Hpy99I CGWCG 1 cut(s) 829
HpyAV CCTTC 2 cut(s) 880, 1029
HpyCH4III ACNGT 3 cut(s) 484, 1139, 1145
HpyCH4IV ACGT 1 cut(s) 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 643, 1025
HpyF3I CTNAG 3 cut(s) 74, 432, 926
HpySE526I ACGT 1 cut(s) 585
Ksp22I TGATCA 3 cut(s) 184, 877, 1117
LmnI GCTCC 3 cut(s) 367, 693, 778
Lsp1109I GCAGC 2 cut(s) 616, 619
LweI GCATC 2 cut(s) 926, 934
MaeI CTAG 3 cut(s) 407, 414, 1089
MaeII ACGT 1 cut(s) 585
MaeIII GTNAC 3 cut(s) 507, 958, 1139
MboII GAAGA 6 cut(s) 65, 94, 170, 300, 337, 750
MfeI CAATTG 3 cut(s) 99, 695, 782
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 364, 901
MlyI GAGTC 2 cut(s) 515, 668
MnlI CCTC 8 cut(s) 302, 441, 480, 702, 726, 769, 1084, 1096
Mph1103I ATGCAT 2 cut(s) 396, 846
MroXI GAANNNNTTC 1 cut(s) 987
MseI TTAA 2 cut(s) 207, 1008
MslI CAYNNNNRTG 2 cut(s) 669, 1010
MspI CCGG 1 cut(s) 234
MspR9I CCNGG 1 cut(s) 27
MunI CAATTG 3 cut(s) 99, 695, 782
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 263, 643, 1025
NcoI CCATGG 1 cut(s) 146
NdeI CATATG 2 cut(s) 736, 840
NmuCI GTSAC 2 cut(s) 507, 958
NsiI ATGCAT 2 cut(s) 396, 846
NspI RCATGY 2 cut(s) 20, 658
NspV TTCGAA 1 cut(s) 92
PagI TCATGA 1 cut(s) 670
PciI ACATGT 1 cut(s) 16
PdmI GAANNNNTTC 1 cut(s) 987
PfeI GAWTC 1 cut(s) 36
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 2 cut(s) 606, 609
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 2 cut(s) 514, 668
PpsI GAGTC 2 cut(s) 514, 668
Ppu21I YACGTR 1 cut(s) 586
PscI ACATGT 1 cut(s) 16
PshAI GACNNNNGTC 2 cut(s) 341, 376
PshBI ATTAAT 1 cut(s) 1008
Psp6I CCWGG 1 cut(s) 25
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 1 cut(s) 25
PspPI GGNCC 1 cut(s) 355
PstNI CAGNNNCTG 1 cut(s) 1143
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 1 cut(s) 527
PvuI CGATCG 1 cut(s) 244
RsaI GTAC 5 cut(s) 21, 200, 227, 650, 1067
RsaNI GTAC 5 cut(s) 20, 199, 226, 649, 1066
RseI CAYNNNNRTG 2 cut(s) 669, 1010
SaqAI TTAA 2 cut(s) 207, 1008
SatI GCNGC 2 cut(s) 605, 608
Sau96I GGNCC 1 cut(s) 355
ScaI AGTACT 1 cut(s) 1067
SchI GAGTC 2 cut(s) 515, 668
ScrFI CCNGG 1 cut(s) 27
SduI GDGCHC 2 cut(s) 364, 901
SfaNI GCATC 2 cut(s) 926, 934
SfcI CTRYAG 1 cut(s) 282
SfuI TTCGAA 1 cut(s) 92
SmiMI CAYNNNNRTG 2 cut(s) 669, 1010
SnaBI TACGTA 1 cut(s) 586
SpeI ACTAGT 1 cut(s) 413
SsiI CCGC 1 cut(s) 800
SspI AATATT 1 cut(s) 1133
SspMI CTAG 3 cut(s) 407, 414, 1089
StyD4I CCNGG 1 cut(s) 25
StyI CCWWGG 2 cut(s) 146, 951
TaaI ACNGT 3 cut(s) 484, 1139, 1145
TaiI ACGT 1 cut(s) 588
TaqI TCGA 5 cut(s) 34, 92, 244, 403, 1105
TatI WGTACW 2 cut(s) 648, 1065
TfiI GAWTC 1 cut(s) 36
Tru1I TTAA 2 cut(s) 207, 1008
Tru9I TTAA 2 cut(s) 207, 1008
TscAI CASTG 2 cut(s) 489, 862
TseFI GTSAC 2 cut(s) 507, 958
TseI GCWGC 2 cut(s) 604, 607
Tsp45I GTSAC 2 cut(s) 507, 958
TspDTI ATGAA 7 cut(s) 66, 207, 411, 526, 980, 1038, 1143
TspRI CASTG 2 cut(s) 489, 862
Tth111I GACNNNGTC 1 cut(s) 527
VspI ATTAAT 1 cut(s) 1008
XapI RAATTY 1 cut(s) 314
XceI RCATGY 2 cut(s) 20, 658
XmnI GAANNNNTTC 1 cut(s) 987
XspI CTAG 3 cut(s) 407, 414, 1089
ZrmI AGTACT 1 cut(s) 1067
Zsp2I ATGCAT 2 cut(s) 396, 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.