Rorug07G0068900
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0008211
Physical Location & Seq
Forward (+)
5231095 .. 5232811
1717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0068900.1

Sequence Viewer

Length: 852 bp
ATGTCTTCTAATACCAAAAGCTTCAGCAGCAGTTCCAGTGAAGATGACAATGAACTTAGGAGAGGGCCATGGACTCTTGAAGAAGACACTCTTCTGATTCAATACATAGCTCGTCACGGTGAAGGCCGATGGAATCTGCTAGCAAATCGGGCAGGATTAAGGAGAACTGGGAAGAGTTGCAGATTGAGATGGTTGAACTATCTGAAACCAGATGTTAAGCGCGGAAATCTTACTCCGGAAGAACAGCTGTTGATTCTTGACCTCCACTCCAAGTGGGGAAATAGGTGGTCGAAAATTGCGCAATACTTACCCGGAAGAACAGACAATGAAATCAAGAACTACTGGAGAACTAGAGTGCAGAAACAAGCAAAGCATCTTAAGATTGATACCAACAGCACAGAGTTTCAGAATATTATTAGGTGCTTTTGGATGCCTAGATTGCTGCAGAAGATAGGAGACCAAAACCAATTAGCTGCTTCTCATCAGCATTCAATAACAGCACCACCATCACAGCCACAATTTACTGAGCAAGGAGCTGTTGACATGAGCGGATTTGTATACGATTTGGATATCGAAAAGCAGAACACAGAATGCATTTTGCCTTCAGAGTTACCAGTGGAGATCTCAAACACTTCTCAATTTTCAGAATATTACCCTTCTAGTTCTTTTGGTGGAGCCATAGTGCGCAACGATCACTATGTAAACAACAATAGCCATGACATGGAAGTGTTCAACTTGGGGAATTCTGCTGCTGCTGATACTTGCTATGTGCCAGAAAGCGAAAGCAACTGGCTTGAAACTGATTTTTCATGTAGCATGTGGAACATGGATGAATTGTTGCAAAGGTGTTAG

Protein Analysis

283

Amino Acids

32.65

Weight (kDa)

5.69

Isoelectric Point (pI)

61.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 21 - 68 1.8e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 24 - 81 1.1e-12 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 74 - 117 1.4e-17 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 78 - 125 7e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 300, 686
AccB7I CCANNNNNTGG 1 cut(s) 721
AccBSI CCGCTC 1 cut(s) 549
AccI GTMKAC 1 cut(s) 558
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 235
AciI CCGC 2 cut(s) 222, 549
AcsI RAATTY 1 cut(s) 742
AcuI CTGAAG 2 cut(s) 7, 588
AfiI CCNNNNNNNGG 1 cut(s) 721
AflII CTTAAG 1 cut(s) 377
AgsI TTSAA 6 cut(s) 80, 101, 196, 492, 733, 797
AluBI AGCT 5 cut(s) 21, 110, 247, 473, 536
AluI AGCT 5 cut(s) 21, 110, 247, 473, 536
Alw26I GTCTC 1 cut(s) 450
Aor13HI TCCGGA 1 cut(s) 235
AoxI GGCC 2 cut(s) 65, 124
ApeKI GCWGC 5 cut(s) 27, 442, 473, 749, 752
ApoI RAATTY 1 cut(s) 742
AspLEI GCGC 3 cut(s) 222, 301, 687
AspS9I GGNCC 1 cut(s) 65
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 1 cut(s) 131
AsuNHI GCTAGC 1 cut(s) 139
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 5 cut(s) 39, 429, 460, 736, 739
BccI CCATC 3 cut(s) 123, 183, 514
BcnI CCSGG 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 4 cut(s) 140, 351, 435, 660
BfmI CTRYAG 1 cut(s) 443
BfrI CTTAAG 1 cut(s) 377
BglII AGATCT 1 cut(s) 621
BisI GCNGC 5 cut(s) 28, 443, 474, 750, 753
BlsI GCNGC 5 cut(s) 29, 444, 475, 751, 754
Bme1390I CCNGG 1 cut(s) 312
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 1 cut(s) 312
BmrI ACTGGG 1 cut(s) 177
BmsI GCATC 2 cut(s) 382, 420
BmtI GCTAGC 1 cut(s) 143
BmuI ACTGGG 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 90
BpmI CTGGAG 1 cut(s) 364
BpuMI CCSGG 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 1 cut(s) 68
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 666, 696
Bsc4I CCNNNNNNNGG 1 cut(s) 721
Bse1I ACTGG 5 cut(s) 36, 172, 347, 614, 794
BseAI TCCGGA 1 cut(s) 235
BseDI CCNNGG 1 cut(s) 68
BseGI GGATG 2 cut(s) 435, 835
BseLI CCNNNNNNNGG 1 cut(s) 721
BseMII CTCAG 1 cut(s) 516
BseNI ACTGG 5 cut(s) 36, 172, 347, 614, 794
BseXI GCAGC 5 cut(s) 39, 429, 460, 736, 739
BsgI GTGCAG 1 cut(s) 377
Bsh1236I CGCG 1 cut(s) 222
BshFI GGCC 2 cut(s) 67, 126
BsiSI CCGG 2 cut(s) 236, 312
BslI CCNNNNNNNGG 1 cut(s) 721
BsmAI GTCTC 1 cut(s) 450
BsmI GAATGC 2 cut(s) 487, 596
BsnI GGCC 2 cut(s) 67, 126
Bso31I GGTCTC 1 cut(s) 450
Bsp13I TCCGGA 1 cut(s) 235
Bsp143I GATC 2 cut(s) 621, 691
Bsp19I CCATGG 1 cut(s) 68
BspACI CCGC 2 cut(s) 222, 549
BspANI GGCC 2 cut(s) 67, 126
BspCNI CTCAG 1 cut(s) 517
BspEI TCCGGA 1 cut(s) 235
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 676
BspMAI CTGCAG 1 cut(s) 447
BspOI GCTAGC 1 cut(s) 143
BspTI CTTAAG 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 450
BsrBI CCGCTC 1 cut(s) 549
BsrI ACTGG 5 cut(s) 36, 172, 347, 614, 794
BssECI CCNNGG 1 cut(s) 68
BssMI GATC 2 cut(s) 621, 691
BssNAI GTATAC 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 68
Bst1107I GTATAC 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 119
Bst6I CTCTTC 2 cut(s) 96, 167
BstAFI CTTAAG 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 56, 525
BstDSI CCRYGG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 435, 835
BstFNI CGCG 1 cut(s) 222
BstHHI GCGC 3 cut(s) 222, 301, 687
BstKTI GATC 2 cut(s) 624, 694
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 2 cut(s) 621, 691
BstMWI GCNNNNNNNGC 3 cut(s) 27, 149, 439
BstNSI RCATGY 1 cut(s) 820
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 443
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 5 cut(s) 39, 429, 460, 736, 739
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BstZ17I GTATAC 1 cut(s) 559
BsuRI GGCC 2 cut(s) 67, 126
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 2 cut(s) 435, 835
BtsIMutI CAGTG 2 cut(s) 43, 621
Cac8I GCNNGC 1 cut(s) 141
CfoI GCGC 3 cut(s) 222, 301, 687
Cfr13I GGNCC 1 cut(s) 65
CviAII CATG 7 cut(s) 69, 544, 716, 721, 810, 817, 826
DdeI CTNAG 2 cut(s) 56, 525
DpnI GATC 2 cut(s) 623, 693
DpnII GATC 2 cut(s) 621, 691
Eam1104I CTCTTC 2 cut(s) 96, 167
EarI CTCTTC 2 cut(s) 96, 167
Eco130I CCWWGG 1 cut(s) 68
Eco31I GGTCTC 1 cut(s) 450
Eco32I GATATC 1 cut(s) 571
Eco57I CTGAAG 2 cut(s) 7, 588
EcoRI GAATTC 1 cut(s) 742
EcoRV GATATC 1 cut(s) 571
EcoT14I CCWWGG 1 cut(s) 68
EcoT22I ATGCAT 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 68
FaeI CATG 7 cut(s) 72, 547, 719, 724, 813, 820, 829
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FatI CATG 7 cut(s) 68, 543, 715, 720, 809, 816, 825
FblI GTMKAC 1 cut(s) 558
Fnu4HI GCNGC 5 cut(s) 28, 443, 474, 750, 753
FokI GGATG 2 cut(s) 442, 842
Fsp4HI GCNGC 5 cut(s) 28, 443, 474, 750, 753
FspBI CTAG 4 cut(s) 140, 351, 435, 660
FspI TGCGCA 2 cut(s) 300, 686
GlaI GCGC 3 cut(s) 221, 300, 686
GluI GCNGC 5 cut(s) 28, 443, 474, 750, 753
GsuI CTGGAG 1 cut(s) 364
HaeIII GGCC 2 cut(s) 67, 126
HapII CCGG 2 cut(s) 236, 312
HhaI GCGC 3 cut(s) 222, 301, 687
Hin1II CATG 7 cut(s) 72, 547, 719, 724, 813, 820, 829
Hin6I GCGC 3 cut(s) 220, 299, 685
HinP1I GCGC 3 cut(s) 220, 299, 685
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HindIII AAGCTT 1 cut(s) 19
HinfI GANTC 4 cut(s) 73, 97, 133, 253
HpaII CCGG 2 cut(s) 236, 312
HphI GGTGA 1 cut(s) 131
Hpy166II GTNNAC 3 cut(s) 541, 559, 703
Hpy188I TCNGA 5 cut(s) 96, 204, 408, 607, 646
Hpy188III TCNNGA 4 cut(s) 77, 236, 257, 334
Hpy8I GTNNAC 3 cut(s) 541, 559, 703
HpyAV CCTTC 3 cut(s) 116, 612, 666
HpyCH4III ACNGT 1 cut(s) 119
HpyCH4V TGCA 5 cut(s) 180, 358, 445, 594, 841
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 149, 439
HpyF3I CTNAG 2 cut(s) 56, 525
Hsp92II CATG 7 cut(s) 72, 547, 719, 724, 813, 820, 829
HspAI GCGC 3 cut(s) 220, 299, 685
Kpn2I TCCGGA 1 cut(s) 235
Kzo9I GATC 2 cut(s) 621, 691
LmnI GCTCC 2 cut(s) 533, 674
Lsp1109I GCAGC 5 cut(s) 39, 429, 460, 736, 739
LweI GCATC 2 cut(s) 382, 420
MaeI CTAG 4 cut(s) 140, 351, 435, 660
MaeIII GTNAC 2 cut(s) 113, 609
MalI GATC 2 cut(s) 623, 693
MbiI CCGCTC 1 cut(s) 549
MboI GATC 2 cut(s) 621, 691
MboII GAAGA 8 cut(s) 53, 83, 92, 95, 184, 251, 327, 460
MflI RGATCY 1 cut(s) 621
MluCI AATT 6 cut(s) 294, 467, 518, 638, 742, 833
MlyI GAGTC 1 cut(s) 67
MnlI CCTC 2 cut(s) 56, 272
Mph1103I ATGCAT 1 cut(s) 596
MroI TCCGGA 1 cut(s) 235
MseI TTAA 3 cut(s) 158, 216, 378
MslI CAYNNNNRTG 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 247
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 2 cut(s) 236, 312
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 2 cut(s) 487, 596
MvnI CGCG 1 cut(s) 222
MwoI GCNNNNNNNGC 3 cut(s) 27, 149, 439
NciI CCSGG 1 cut(s) 312
NcoI CCATGG 1 cut(s) 68
NdeII GATC 2 cut(s) 621, 691
NheI GCTAGC 1 cut(s) 139
NlaIII CATG 7 cut(s) 72, 547, 719, 724, 813, 820, 829
NlaIV GGNNCC 1 cut(s) 676
NmuCI GTSAC 1 cut(s) 113
NsbI TGCGCA 2 cut(s) 300, 686
NsiI ATGCAT 1 cut(s) 596
NspI RCATGY 1 cut(s) 820
PctI GAATGC 2 cut(s) 487, 596
PfeI GAWTC 3 cut(s) 97, 133, 253
PflMI CCANNNNNTGG 1 cut(s) 721
PkrI GCNGC 5 cut(s) 29, 444, 475, 751, 754
PleI GAGTC 1 cut(s) 67
PpsI GAGTC 1 cut(s) 67
PspN4I GGNNCC 1 cut(s) 676
PspPI GGNCC 1 cut(s) 65
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 1 cut(s) 621
PvuII CAGCTG 1 cut(s) 247
RseI CAYNNNNRTG 1 cut(s) 725
SaqAI TTAA 3 cut(s) 158, 216, 378
SatI GCNGC 5 cut(s) 28, 443, 474, 750, 753
Sau3AI GATC 2 cut(s) 621, 691
Sau96I GGNCC 1 cut(s) 65
SchI GAGTC 1 cut(s) 67
ScrFI CCNGG 1 cut(s) 312
SetI ASST 9 cut(s) 23, 112, 249, 264, 287, 422, 475, 538, 848
SfaNI GCATC 2 cut(s) 382, 420
SfcI CTRYAG 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
Sse9I AATT 6 cut(s) 294, 467, 518, 638, 742, 833
SsiI CCGC 2 cut(s) 222, 549
SspI AATATT 2 cut(s) 412, 650
SspMI CTAG 4 cut(s) 140, 351, 435, 660
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 68
TaaI ACNGT 1 cut(s) 119
TaqI TCGA 2 cut(s) 290, 573
TasI AATT 6 cut(s) 294, 467, 518, 638, 742, 833
TfiI GAWTC 3 cut(s) 97, 133, 253
Tru1I TTAA 3 cut(s) 158, 216, 378
Tru9I TTAA 3 cut(s) 158, 216, 378
TscAI CASTG 2 cut(s) 43, 621
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 5 cut(s) 27, 442, 473, 749, 752
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 4 cut(s) 66, 342, 798, 846
TspRI CASTG 2 cut(s) 43, 621
Van91I CCANNNNNTGG 1 cut(s) 721
Vha464I CTTAAG 1 cut(s) 377
XapI RAATTY 1 cut(s) 742
XceI RCATGY 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 558
XspI CTAG 4 cut(s) 140, 351, 435, 660
Zsp2I ATGCAT 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.