pycom06g08810

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0005021
Physical Location & Seq
Forward (+)
13998926 .. 14000307
1382 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g08810.4

Sequence Viewer

Length: 966 bp
ATGAAGCAGAACAAATTGCAATATATCACAACTGTCCCCGATGTTTCCCAATATATCACCGAACACCCCAACCAGCCTTCTAAACGCTGCGTTTCGTGTCCTCCAGACTCTCAGACGCGAATCGCGATGTCCGGTGATTCGAAGCTCTCGCGGCTGTGCCGTTTGGACGACGATTTTCTCCGTCCGTACACGTCAGAACCCGGCGTCGTTTTGGTAGACCAAGAGATGGAAGTTCCGGTCACTCTCTCTCAGGTTCTTCGACAAGTCAAGCTCTGGACCCAAGCACTTGATTCCGGCTCTGAAAATGGAATGGCGATAGGATTGGATTGTAAGAGTGATTGTATGTGTCCGACTAAAATCGTAACTCACTTGAGTCAATATGTCAAGCATCTGGCGTGCAATGTTCTTGTTGCTGTGTCGGAAGTCCTTGTTGATTCGGGTTTTATCCGTTTGTTGTGTGTTTGTATGAATTTGGCGATCATTGTGGCGGTGTCTTGTTCTTCATCATATTCGGTGGATGGAGCTGATGATCTTCATTCTGGTTCCTCGAGGTTGATTATTGCGTTAAAGCCTAAGCTGGGAAATGCTGACTGGTCTATTGTGGCTGGTGTAGTTCGGGTTTTACATGATATAAGGAAATATTTGAAGTCAGAAGACGATGATGAACTTGTTGAAGCATTCTTTGATTCTATCAATTCCGTTCTTTCAACTGTGCCTTGGAATTTACTAGATGAGATTCATGTTGGTGCAAAGCCTGATGTTCAGAAAGGGTCCAGAGCAGATGCTTTGTTTCAAATATCCTTGTTTCTAGGAAATTTGATTCAGTTTCTCTGTTCCATGGTTGAGCAAAGTGGTTCTGCGGAAGCTTCAGGTCGATCTATAGACAAACATCACCCAGTTTTTTCTACGTTCATCACCCTTGTTCTGCAGCTTTTATGTTGGTGCCTTGGTGAGCCATTGCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LINES_C LINES_N

Protein Analysis

322

Amino Acids

35.3

Weight (kDa)

5.34

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 547
AccB1I GGYRCC 1 cut(s) 942
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 1 cut(s) 216
AccII CGCG 3 cut(s) 118, 125, 151
AciI CCGC 3 cut(s) 151, 488, 860
AcsI RAATTY 3 cut(s) 469, 721, 814
AcuI CTGAAG 1 cut(s) 852
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 2 cut(s) 226, 578
AflIII ACRYGT 1 cut(s) 189
AgsI TTSAA 4 cut(s) 646, 674, 708, 794
AjiI CACGTC 1 cut(s) 192
AluBI AGCT 6 cut(s) 145, 271, 524, 577, 866, 931
AluI AGCT 6 cut(s) 145, 271, 524, 577, 866, 931
Ama87I CYCGRG 1 cut(s) 547
ApeKI GCWGC 2 cut(s) 87, 928
ApoI RAATTY 3 cut(s) 469, 721, 814
AspS9I GGNCC 2 cut(s) 276, 771
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 5 cut(s) 49, 146, 884, 907, 962
AsuII TTCGAA 1 cut(s) 140
AvaI CYCGRG 1 cut(s) 547
AvaII GGWCC 2 cut(s) 276, 771
BanI GGYRCC 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 660
BbvI GCAGC 2 cut(s) 74, 940
BccI CCATC 2 cut(s) 220, 512
BceAI ACGGC 1 cut(s) 144
BcnI CCSGG 1 cut(s) 201
BfaI CTAG 2 cut(s) 728, 809
BfmI CTRYAG 2 cut(s) 879, 926
BisI GCNGC 3 cut(s) 88, 152, 929
BlsI GCNGC 3 cut(s) 89, 153, 930
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 2 cut(s) 276, 771
BmeT110I CYCGRG 1 cut(s) 547
BmgBI CACGTC 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 276, 771
BmiI GGNNCC 4 cut(s) 278, 544, 772, 944
BmrFI CCNGG 1 cut(s) 201
BmrI ACTGGG 1 cut(s) 890
BmsI GCATC 2 cut(s) 397, 772
BmuI ACTGGG 1 cut(s) 890
BpiI GAAGAC 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 87
Bpu10I CCTNAGC 1 cut(s) 573
Bpu14I TTCGAA 1 cut(s) 140
BpuEI CTTGAG 1 cut(s) 391
BpuMI CCSGG 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 716, 837, 946
BsaWI WCCGGW 2 cut(s) 131, 235
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 578
Bse1I ACTGG 2 cut(s) 596, 896
Bse3DI GCAATG 2 cut(s) 406, 956
BseDI CCNNGG 3 cut(s) 716, 837, 946
BseGI GGATG 1 cut(s) 523
BseLI CCNNNNNNNGG 2 cut(s) 226, 578
BseMI GCAATG 2 cut(s) 406, 956
BseMII CTCAG 2 cut(s) 125, 263
BseNI ACTGG 2 cut(s) 596, 896
BseXI GCAGC 2 cut(s) 74, 940
BseYI CCCAGC 1 cut(s) 577
Bsh1236I CGCG 3 cut(s) 118, 125, 151
BshNI GGYRCC 1 cut(s) 942
BsiHKCI CYCGRG 1 cut(s) 547
BsiSI CCGG 4 cut(s) 132, 201, 236, 294
BslFI GGGAC 1 cut(s) 20
BslI CCNNNNNNNGG 2 cut(s) 226, 578
BsmFI GGGAC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 677
BsoBI CYCGRG 1 cut(s) 547
Bsp119I TTCGAA 1 cut(s) 140
Bsp143I GATC 3 cut(s) 477, 529, 875
Bsp19I CCATGG 1 cut(s) 837
Bsp68I TCGCGA 1 cut(s) 125
BspACI CCGC 3 cut(s) 151, 488, 860
BspCNI CTCAG 2 cut(s) 124, 262
BspFNI CGCG 3 cut(s) 118, 125, 151
BspLI GGNNCC 4 cut(s) 278, 544, 772, 944
BspMAI CTGCAG 1 cut(s) 930
BspT104I TTCGAA 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 942
BsrDI GCAATG 2 cut(s) 406, 956
BsrI ACTGG 2 cut(s) 596, 896
BssECI CCNNGG 3 cut(s) 716, 837, 946
BssMI GATC 3 cut(s) 477, 529, 875
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 3 cut(s) 716, 837, 946
Bst4CI ACNGT 2 cut(s) 34, 712
BstACI GRCGYC 1 cut(s) 204
BstBI TTCGAA 1 cut(s) 140
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 3 cut(s) 111, 249, 573
BstDSI CCRYGG 1 cut(s) 837
BstF5I GGATG 1 cut(s) 523
BstFNI CGCG 3 cut(s) 118, 125, 151
BstKTI GATC 3 cut(s) 480, 532, 878
BstMBI GATC 3 cut(s) 477, 529, 875
BstMWI GCNNNNNNNGC 1 cut(s) 151
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 2 cut(s) 879, 926
BstUI CGCG 3 cut(s) 118, 125, 151
BstV1I GCAGC 2 cut(s) 74, 940
BstV2I GAAGAC 1 cut(s) 660
BtgI CCRYGG 1 cut(s) 837
BtgZI GCGATG 1 cut(s) 140
BtrI CACGTC 1 cut(s) 192
BtsCI GGATG 1 cut(s) 523
BtuMI TCGCGA 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 276, 771
CseI GACGC 2 cut(s) 124, 193
Csp6I GTAC 1 cut(s) 187
CviAII CATG 3 cut(s) 626, 740, 838
CviQI GTAC 1 cut(s) 187
DdeI CTNAG 3 cut(s) 111, 249, 573
DpnI GATC 3 cut(s) 479, 531, 877
DpnII GATC 3 cut(s) 477, 529, 875
Eco130I CCWWGG 3 cut(s) 716, 837, 946
Eco47I GGWCC 2 cut(s) 276, 771
Eco57I CTGAAG 1 cut(s) 852
Eco88I CYCGRG 1 cut(s) 547
EcoT14I CCWWGG 3 cut(s) 716, 837, 946
ErhI CCWWGG 3 cut(s) 716, 837, 946
FaeI CATG 3 cut(s) 629, 743, 841
FaqI GGGAC 1 cut(s) 20
FatI CATG 3 cut(s) 625, 739, 837
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 3 cut(s) 88, 152, 929
FokI GGATG 1 cut(s) 530
Fsp4HI GCNGC 3 cut(s) 88, 152, 929
FspBI CTAG 2 cut(s) 728, 809
GluI GCNGC 3 cut(s) 88, 152, 929
GsaI CCCAGC 1 cut(s) 581
GsuI CTGGAG 1 cut(s) 87
HapII CCGG 4 cut(s) 132, 201, 236, 294
HgaI GACGC 2 cut(s) 124, 193
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 3 cut(s) 629, 743, 841
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 9 cut(s) 107, 120, 137, 290, 373, 434, 686, 736, 820
HpaII CCGG 4 cut(s) 132, 201, 236, 294
HphI GGTGA 5 cut(s) 49, 146, 884, 907, 962
Hpy166II GTNNAC 2 cut(s) 189, 217
Hpy188I TCNGA 7 cut(s) 114, 196, 301, 351, 421, 652, 765
Hpy188III TCNNGA 4 cut(s) 104, 124, 274, 774
Hpy8I GTNNAC 2 cut(s) 189, 217
Hpy99I CGWCG 2 cut(s) 173, 209
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 2 cut(s) 34, 712
HpyCH4IV ACGT 2 cut(s) 191, 908
HpyCH4V TGCA 5 cut(s) 19, 399, 749, 928, 961
HpyF10VI GCNNNNNNNGC 1 cut(s) 151
HpyF3I CTNAG 3 cut(s) 111, 249, 573
HpySE526I ACGT 2 cut(s) 191, 908
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 3 cut(s) 629, 743, 841
Kzo9I GATC 3 cut(s) 477, 529, 875
LmnI GCTCC 1 cut(s) 521
Lsp1109I GCAGC 2 cut(s) 74, 940
LweI GCATC 2 cut(s) 397, 772
MaeI CTAG 2 cut(s) 728, 809
MaeII ACGT 2 cut(s) 191, 908
MaeIII GTNAC 2 cut(s) 238, 361
MalI GATC 3 cut(s) 479, 531, 877
MboI GATC 3 cut(s) 477, 529, 875
MboII GAAGA 4 cut(s) 248, 492, 524, 665
MluCI AATT 5 cut(s) 14, 469, 694, 721, 814
MlyI GAGTC 2 cut(s) 101, 382
MmeI TCCRAC 2 cut(s) 374, 399
MnlI CCTC 3 cut(s) 111, 543, 556
MseI TTAA 2 cut(s) 566, 964
MslI CAYNNNNRTG 1 cut(s) 744
MspI CCGG 4 cut(s) 132, 201, 236, 294
MspR9I CCNGG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 677
MvnI CGCG 3 cut(s) 118, 125, 151
MwoI GCNNNNNNNGC 1 cut(s) 151
NciI CCSGG 1 cut(s) 201
NcoI CCATGG 1 cut(s) 837
NdeII GATC 3 cut(s) 477, 529, 875
NlaIII CATG 3 cut(s) 629, 743, 841
NlaIV GGNNCC 4 cut(s) 278, 544, 772, 944
NmuCI GTSAC 1 cut(s) 238
NruI TCGCGA 1 cut(s) 125
NspV TTCGAA 1 cut(s) 140
PaeR7I CTCGAG 1 cut(s) 547
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PctI GAATGC 1 cut(s) 677
PfeI GAWTC 7 cut(s) 120, 137, 290, 434, 686, 736, 820
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 3 cut(s) 89, 153, 930
PleI GAGTC 2 cut(s) 101, 381
PpsI GAGTC 2 cut(s) 101, 381
PspFI CCCAGC 1 cut(s) 577
PspN4I GGNNCC 4 cut(s) 278, 544, 772, 944
PspPI GGNCC 2 cut(s) 276, 771
PspXI VCTCGAGB 1 cut(s) 547
PstI CTGCAG 1 cut(s) 930
RruI TCGCGA 1 cut(s) 125
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
RseI CAYNNNNRTG 1 cut(s) 744
SaqAI TTAA 2 cut(s) 566, 964
SatI GCNGC 3 cut(s) 88, 152, 929
Sau3AI GATC 3 cut(s) 477, 529, 875
Sau96I GGNCC 2 cut(s) 276, 771
SchI GAGTC 2 cut(s) 101, 382
ScrFI CCNGG 1 cut(s) 201
SfaNI GCATC 2 cut(s) 397, 772
SfcI CTRYAG 2 cut(s) 879, 926
Sfr274I CTCGAG 1 cut(s) 547
SfuI TTCGAA 1 cut(s) 140
SinI GGWCC 2 cut(s) 276, 771
SlaI CTCGAG 1 cut(s) 547
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 2 cut(s) 370, 547
SmoI CTYRAG 2 cut(s) 370, 547
Sse9I AATT 5 cut(s) 14, 469, 694, 721, 814
SsiI CCGC 3 cut(s) 151, 488, 860
SspI AATATT 1 cut(s) 641
SspMI CTAG 2 cut(s) 728, 809
StyD4I CCNGG 1 cut(s) 199
StyI CCWWGG 3 cut(s) 716, 837, 946
TaaI ACNGT 2 cut(s) 34, 712
TaiI ACGT 2 cut(s) 194, 911
TaqI TCGA 4 cut(s) 140, 259, 548, 874
TasI AATT 5 cut(s) 14, 469, 694, 721, 814
TauI GCSGC 1 cut(s) 154
TfiI GAWTC 7 cut(s) 120, 137, 290, 434, 686, 736, 820
Tru1I TTAA 2 cut(s) 566, 964
Tru9I TTAA 2 cut(s) 566, 964
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 87, 928
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 7 cut(s) 17, 482, 492, 524, 678, 728, 901
TspGWI ACGGA 4 cut(s) 170, 174, 437, 688
Van91I CCANNNNNTGG 1 cut(s) 226
VpaK11BI GGWCC 2 cut(s) 276, 771
XapI RAATTY 3 cut(s) 469, 721, 814
XhoI CTCGAG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 216
XspI CTAG 2 cut(s) 728, 809
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.