Rh1BG081300

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005021
Physical Location & Seq
Forward (+)
12840651 .. 12843873
3223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG081300.1

Sequence Viewer

Length: 1461 bp
ATGAAGCGTCGATTCAAAATGTGCAGCGATTCAAATCTGACGAAGCTCCACCGTCTGATCCACGATTCTCTCCGTCCGTACACAGAACCAGATGTCGTTTCGGTGACGAAGGAGGGAGAAATTCTGGTCAGTCTCTCTCAGATTCTTCGGCAAGTGAAGCTCTGGATTGGAGAAGTTGATTCCGATTCTGATAATGGAGTGGTGATTGGATTGGATTTTGAGAATGAGTGTATTGGAGAGTCAATATGTGAAGCATCTGGGCTAGCTGCTGTCTCCGAATTCGTGGCGGCTTCGGGAAGCCACTGGGATGCCTTTATTCGTTTGTTGTGTGATTGTATGGATTTGGCAATCACTGCGGCAGTTTCATGTTCTACATCGACTTTGGCAACTGGAGCTAGTGATCTTTGTTCTAGTTCATTGAGTTTAATTCTCGTGCTGAAACCTAAGGTGAAAAATGGTGACTGGTCTGTAGCGGCTGGTGTTGTTCGGGTGCTACGTGATATACTGAAGTATTTGAAGTCTGAGGATGATGACGAACTTGTTCAAGTGTTCATTGAGGCTGTTAATTCTTTTCTTTCAACAGTGCCTTGGGATTTATTGCATGAGATTCGTGTTGGTCCAAATGCTGATGCTCTGAAATGCTCCAGGGCAGATGTTTCATTCCAAAGAACCTTTTTCTCTGTTCCTTGGTCGAGCAAGGCAGTGTTGTACGTCGATGCTCAAGGTGGATCTCTAGACAAGCATCATCCAGTGTTTTCTAGAATCATCAACCTTGTACCTAAGCTTTTACGTTTATGCCTTGGGGAGCAGGCGGATTGTGTTATCTCTCACAACTGCATCTCTCAATACTTTAAACACAAGTTATTGGTGCTAATGATCAGGCTCATTGTCCAAACTTGTCCAGAATCCTCAATTCTTGTTTCATGGTTGCAACTTATCCATTATTACTTTGAAGAGCTTTTGCGGCAACCCATTTCTACTCTGGAGTGTAATCAGGAAGATTGTATGGAAGGCTCTCCATTTCTATCCAGTGTTTCTGATTCAGAATGTTGTGGAAGAAAGGAAGGACTGTTGGAGCTTAATAACTGGCTTCAAGGACATCTTCCAACTGATATGTTTGTGGACCATGAAATGTATTTTGAAAAATGTGCAGACTTTGCCAAATCCTTCCTCCAACTATATATCAAAGAGGATGATGTATTATTCAGAGTGCTCTTGCAATTGCTTTGTGTACCTTGTCCTGCAGAGAAACAGTTTGAAGAGGAAAGGGGATCATTTCAGGATTCAAAGGGCAATGTGCTTTTCTTTGTTTCAGATCTTTTCAATCCTGTACTCTTATTCCATCTATTTCTTGTAGAGTTAAGCTATGACCATCAAGTGCTTCTTGATTACCTTATTACAAAAGATACTGGAATCAGTTGTGCAGAATATCTTTTAAGTTCGCTGGTATTAGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LINES_C LINES_N

Protein Analysis

486

Amino Acids

54.63

Weight (kDa)

4.86

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LINES_N PF14694 371 - 471 3.2e-07 Lines N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 287, 356, 473, 812, 964
AclWI GGATC 3 cut(s) 52, 736, 1279
AcsI RAATTY 2 cut(s) 120, 278
AcuI CTGAAG 1 cut(s) 527
AfaI GTAC 5 cut(s) 80, 710, 777, 1233, 1332
AjnI CCWGG 1 cut(s) 644
AluBI AGCT 8 cut(s) 46, 160, 266, 395, 784, 958, 1078, 1365
AluI AGCT 8 cut(s) 46, 160, 266, 395, 784, 958, 1078, 1365
Alw21I GWGCWC 1 cut(s) 1215
Alw26I GTCTC 2 cut(s) 137, 277
AlwI GGATC 3 cut(s) 52, 736, 1279
ApeKI GCWGC 2 cut(s) 24, 266
ApoI RAATTY 2 cut(s) 120, 278
Asp700I GAANNNNTTC 1 cut(s) 540
AspS9I GGNCC 2 cut(s) 617, 1123
AsuHPI GGTGA 4 cut(s) 115, 214, 460, 470
AsuNHI GCTAGC 1 cut(s) 262
AvaII GGWCC 2 cut(s) 617, 1123
AxyI CCTNAGG 1 cut(s) 444
BauI CACGAG 1 cut(s) 431
Bbv12I GWGCWC 1 cut(s) 1215
BbvI GCAGC 2 cut(s) 36, 253
BccI CCATC 2 cut(s) 1350, 1380
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 1 cut(s) 646
BclI TGATCA 1 cut(s) 876
BcoDI GTCTC 2 cut(s) 137, 277
BfaI CTAG 5 cut(s) 263, 396, 411, 734, 759
BfmI CTRYAG 2 cut(s) 468, 1242
BglII AGATCT 1 cut(s) 1315
BisI GCNGC 6 cut(s) 25, 267, 288, 357, 474, 965
BlsI GCNGC 6 cut(s) 26, 268, 289, 358, 475, 966
Bme1390I CCNGG 1 cut(s) 646
Bme18I GGWCC 2 cut(s) 617, 1123
BmgT120I GGNCC 2 cut(s) 617, 1123
BmrFI CCNGG 1 cut(s) 646
BmrI ACTGGG 1 cut(s) 313
BmsI GCATC 6 cut(s) 263, 298, 619, 706, 751, 846
BmtI GCTAGC 1 cut(s) 266
BmuI ACTGGG 1 cut(s) 313
BpmI CTGGAG 3 cut(s) 411, 628, 1004
Bpu10I CCTNAGC 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 4 cut(s) 587, 645, 686, 799
Bse1I ACTGG 7 cut(s) 308, 394, 467, 749, 1029, 1091, 1414
Bse21I CCTNAGG 1 cut(s) 444
Bse3DI GCAATG 1 cut(s) 1300
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 1 cut(s) 646
BseDI CCNNGG 4 cut(s) 587, 645, 686, 799
BseGI GGATG 4 cut(s) 313, 532, 745, 1198
BseJI GATNNNNATC 1 cut(s) 33
BseMI GCAATG 1 cut(s) 1300
BseMII CTCAG 2 cut(s) 152, 513
BseNI ACTGG 7 cut(s) 308, 394, 467, 749, 1029, 1091, 1414
BseXI GCAGC 2 cut(s) 36, 253
BsgI GTGCAG 3 cut(s) 43, 1170, 1443
BsiHKAI GWGCWC 1 cut(s) 1215
BsmAI GTCTC 2 cut(s) 137, 277
Bsp1286I GDGCHC 1 cut(s) 1215
Bsp143I GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
BspACI CCGC 5 cut(s) 287, 356, 473, 812, 964
BspCNI CTCAG 2 cut(s) 151, 514
BspMAI CTGCAG 1 cut(s) 1246
BspOI GCTAGC 1 cut(s) 266
BspPI GGATC 3 cut(s) 52, 736, 1279
BspQI GCTCTTC 1 cut(s) 948
BsrDI GCAATG 1 cut(s) 1300
BsrI ACTGG 7 cut(s) 308, 394, 467, 749, 1029, 1091, 1414
BssECI CCNNGG 4 cut(s) 587, 645, 686, 799
BssMI GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
BssSI CACGAG 1 cut(s) 431
BssT1I CCWWGG 3 cut(s) 587, 686, 799
Bst2BI CACGAG 1 cut(s) 431
Bst2UI CCWGG 1 cut(s) 646
Bst4CI ACNGT 4 cut(s) 53, 583, 1071, 1254
Bst6I CTCTTC 2 cut(s) 948, 1254
BstAPI GCANNNNNTGC 2 cut(s) 353, 1157
BstBAI YACGTR 1 cut(s) 497
BstC8I GCNNGC 2 cut(s) 264, 810
BstDEI CTNAG 4 cut(s) 138, 444, 522, 780
BstF5I GGATG 4 cut(s) 313, 532, 745, 1198
BstKTI GATC 6 cut(s) 60, 403, 731, 879, 1274, 1318
BstMAI GTCTC 2 cut(s) 137, 277
BstMBI GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
BstMWI GCNNNNNNNGC 5 cut(s) 157, 353, 392, 964, 1157
BstNI CCWGG 1 cut(s) 646
BstSCI CCNGG 1 cut(s) 644
BstSFI CTRYAG 2 cut(s) 468, 1242
BstV1I GCAGC 2 cut(s) 36, 253
BstX2I RGATCY 2 cut(s) 728, 1315
BstYI RGATCY 2 cut(s) 728, 1315
Bsu36I CCTNAGG 1 cut(s) 444
BtsCI GGATG 4 cut(s) 313, 532, 745, 1198
BtsI GCAGTG 2 cut(s) 351, 708
BtsIMutI CAGTG 6 cut(s) 301, 351, 588, 708, 756, 1036
Cac8I GCNNGC 2 cut(s) 264, 810
Cfr13I GGNCC 2 cut(s) 617, 1123
Csp6I GTAC 5 cut(s) 79, 709, 776, 1232, 1331
CviAII CATG 4 cut(s) 366, 602, 924, 1127
CviQI GTAC 5 cut(s) 79, 709, 776, 1232, 1331
DdeI CTNAG 4 cut(s) 138, 444, 522, 780
DpnI GATC 6 cut(s) 59, 402, 730, 878, 1273, 1317
DpnII GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
DraI TTTAAA 1 cut(s) 853
Eam1104I CTCTTC 2 cut(s) 948, 1254
EarI CTCTTC 2 cut(s) 948, 1254
EciI GGCGGA 1 cut(s) 827
Eco130I CCWWGG 3 cut(s) 587, 686, 799
Eco47I GGWCC 2 cut(s) 617, 1123
Eco57I CTGAAG 1 cut(s) 527
Eco81I CCTNAGG 1 cut(s) 444
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 644
EcoT14I CCWWGG 3 cut(s) 587, 686, 799
ErhI CCWWGG 3 cut(s) 587, 686, 799
FaeI CATG 4 cut(s) 369, 605, 927, 1130
FalI AAGNNNNNCTT 4 cut(s) 1086, 1118, 1368, 1400
FatI CATG 4 cut(s) 365, 601, 923, 1126
FbaI TGATCA 1 cut(s) 876
Fnu4HI GCNGC 6 cut(s) 25, 267, 288, 357, 474, 965
FokI GGATG 4 cut(s) 320, 539, 732, 1205
Fsp4HI GCNGC 6 cut(s) 25, 267, 288, 357, 474, 965
FspBI CTAG 5 cut(s) 263, 396, 411, 734, 759
GluI GCNGC 6 cut(s) 25, 267, 288, 357, 474, 965
GsuI CTGGAG 3 cut(s) 411, 628, 1004
Hin1II CATG 4 cut(s) 369, 605, 927, 1130
HindIII AAGCTT 1 cut(s) 782
HphI GGTGA 4 cut(s) 115, 214, 460, 470
Hpy166II GTNNAC 3 cut(s) 81, 1123, 1232
Hpy188III TCNNGA 9 cut(s) 163, 294, 734, 759, 902, 983, 995, 1280, 1385
Hpy8I GTNNAC 3 cut(s) 81, 1123, 1232
Hpy99I CGWCG 2 cut(s) 12, 716
HpyAV CCTTC 4 cut(s) 103, 1004, 1058, 1177
HpyCH4III ACNGT 4 cut(s) 53, 583, 1071, 1254
HpyCH4IV ACGT 3 cut(s) 496, 711, 790
HpyCH4V TGCA 8 cut(s) 24, 601, 837, 931, 1151, 1219, 1244, 1424
HpyF10VI GCNNNNNNNGC 5 cut(s) 157, 353, 392, 964, 1157
HpyF3I CTNAG 4 cut(s) 138, 444, 522, 780
HpySE526I ACGT 3 cut(s) 496, 711, 790
Hsp92II CATG 4 cut(s) 369, 605, 927, 1130
Ksp22I TGATCA 1 cut(s) 876
Kzo9I GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
LguI GCTCTTC 1 cut(s) 948
LmnI GCTCC 5 cut(s) 51, 392, 647, 805, 1075
Lsp1109I GCAGC 2 cut(s) 36, 253
LweI GCATC 6 cut(s) 263, 298, 619, 706, 751, 846
MaeI CTAG 5 cut(s) 263, 396, 411, 734, 759
MaeII ACGT 3 cut(s) 496, 711, 790
MaeIII GTNAC 2 cut(s) 103, 458
MalI GATC 6 cut(s) 59, 402, 730, 878, 1273, 1317
MboI GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
MboII GAAGA 6 cut(s) 137, 965, 1010, 1068, 1094, 1271
MfeI CAATTG 1 cut(s) 1220
MflI RGATCY 2 cut(s) 728, 1315
MhlI GDGCHC 1 cut(s) 1215
MluCI AATT 6 cut(s) 120, 278, 426, 565, 912, 1220
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 3 cut(s) 1053, 1130, 1198
MnlI CCTC 7 cut(s) 106, 517, 550, 919, 1181, 1183, 1255
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 6 cut(s) 425, 564, 852, 1080, 1361, 1436
MslI CAYNNNNRTG 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 646
MunI CAATTG 1 cut(s) 1220
MvaI CCWGG 1 cut(s) 646
MwoI GCNNNNNNNGC 5 cut(s) 157, 353, 392, 964, 1157
NdeII GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
NheI GCTAGC 1 cut(s) 262
NlaIII CATG 4 cut(s) 369, 605, 927, 1130
NmuCI GTSAC 2 cut(s) 103, 458
PciSI GCTCTTC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 493
PdmI GAANNNNTTC 1 cut(s) 540
PkrI GCNGC 6 cut(s) 26, 268, 289, 358, 475, 966
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Ppu21I YACGTR 1 cut(s) 497
Psp6I CCWGG 1 cut(s) 644
PspGI CCWGG 1 cut(s) 644
PspPI GGNCC 2 cut(s) 617, 1123
PstI CTGCAG 1 cut(s) 1246
PsuI RGATCY 2 cut(s) 728, 1315
RsaI GTAC 5 cut(s) 80, 710, 777, 1233, 1332
RsaNI GTAC 5 cut(s) 79, 709, 776, 1232, 1331
RseI CAYNNNNRTG 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 948
SaqAI TTAA 6 cut(s) 425, 564, 852, 1080, 1361, 1436
SatI GCNGC 6 cut(s) 25, 267, 288, 357, 474, 965
Sau3AI GATC 6 cut(s) 57, 400, 728, 876, 1271, 1315
Sau96I GGNCC 2 cut(s) 617, 1123
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 1 cut(s) 646
SduI GDGCHC 1 cut(s) 1215
SfaNI GCATC 6 cut(s) 263, 298, 619, 706, 751, 846
SfcI CTRYAG 2 cut(s) 468, 1242
SinI GGWCC 2 cut(s) 617, 1123
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 1 cut(s) 720
SmoI CTYRAG 1 cut(s) 720
Sse9I AATT 6 cut(s) 120, 278, 426, 565, 912, 1220
SsiI CCGC 5 cut(s) 287, 356, 473, 812, 964
SspMI CTAG 5 cut(s) 263, 396, 411, 734, 759
StyD4I CCNGG 1 cut(s) 644
StyI CCWWGG 3 cut(s) 587, 686, 799
TaaI ACNGT 4 cut(s) 53, 583, 1071, 1254
TaiI ACGT 3 cut(s) 499, 714, 793
TaqI TCGA 4 cut(s) 10, 377, 692, 714
TasI AATT 6 cut(s) 120, 278, 426, 565, 912, 1220
TatI WGTACW 1 cut(s) 1330
TauI GCSGC 4 cut(s) 290, 359, 476, 967
Tru1I TTAA 6 cut(s) 425, 564, 852, 1080, 1361, 1436
Tru9I TTAA 6 cut(s) 425, 564, 852, 1080, 1361, 1436
TscAI CASTG 6 cut(s) 308, 358, 588, 708, 756, 1036
TseFI GTSAC 2 cut(s) 103, 458
TseI GCWGC 2 cut(s) 24, 266
Tsp45I GTSAC 2 cut(s) 103, 458
TspDTI ATGAA 7 cut(s) 17, 354, 405, 541, 648, 912, 1143
TspGWI ACGGA 2 cut(s) 62, 66
TspRI CASTG 6 cut(s) 308, 358, 588, 708, 756, 1036
VpaK11BI GGWCC 2 cut(s) 617, 1123
XapI RAATTY 2 cut(s) 120, 278
XbaI TCTAGA 2 cut(s) 733, 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 5 cut(s) 263, 396, 411, 734, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.