Rw4G001250

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0005021
Physical Location & Seq
Forward (+)
2303548 .. 2308692
5145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001250.1

Sequence Viewer

Length: 1746 bp
ATGACTCTTGATTGCTGGGAAACATTACAGTACCTTTTTCTGTCAGAAAACAATTTTGTTAGTCTTCCTGCTTGCATCACCAGATATGTCAACTTATGGCGGCTTGACTTGTACGGTTGCAAGAGACTCCGAGAAATTCCGGAGATCCCACCAGACGGAGTGGTAGTTGGATTGGATTGTGGCAATGTGTGTCTGAGTAAAATCTTAACTCACTTGATTGTTTTGCTAACAGTGGAGAGTCAATATGTGAAGCATCTGGCCTGCAATGTTCTAACTGTTGTCTCCGAATTCGTGGCGGCTTCTGGAAGCCACTGGGATGCCTTTATTCGTTTGTTGTGTGATTGTGTGGATTTGGCAATCACTGCGGCAGTTTCATGTTCTACATCGACTTTGGCAACTGGAGCTAGTGATCTTTGTTCTAGTTCATTGAGTTTAATTCTCATGCTGAAACCTAAGATGAAAAATGGTGACTGGTCTGTAGCGGCTGGTGTTGTTCGGGTGCTACGTGATATACTGAAGTTTTTGAAGTCTGAGGATGATGATGAACTTGTTGAAGTGTTCATTGAGGCTGTTAATTCTTTTCTTTCAACAGTGCCTTGGGATTTATTGCATGAGATTCGCGTTGGTCCAACTGCTGATGCTCTGAAATGCTCCAGGGCAGATGTTTCATTCCAAAGAACCTTGTTTCTAGGAAACTTGATTCAGTTTCTCTGTTCCTTGGTCGAGCAAGGCGGTGTTGTCGATGCTCCAGGTGGATCTCTAGACAAGCATCATCCAGTGTTTTCTGGAATCATCAACCTTGTACCTAAGCTTTTACGTTTATGCCTTGGGGAGCAAGCGGATTGTGTTATCTCTAACAACTGCATCTCTCAATACTTTAAACACAAGTTATTGGTGCTAATGATCAGGCTCATTGTCCAAACTTGTCCAGAATCCTCAATTCTTGTTTCATGGTTGCAACTTATCCATTATTACTTTGAAGAGCTTTTGCGGCAACCCATTTCTACTCTGGAGTGTAATCAGGAAGATTGTTTGGAAGGCTCTCCATTTCTATCCGGTGTTTCTGATTCAGAAGTAAATAGTCTGTCTTCTCGCCATCTGCAAAGACAGGCTGTGTTCCTTTTTCTGAGGTTTCTTTTAAAAATGTGCTTGTGCAAGTTGGAATTATGCTTGGACTATGACTCAAATGCAGAGTTGCAGTGTTGTGGAAGAAAGGAAGGACTGTTGGAGCTTAATAACTGGCTTCAAGGACGTCTTCCAACTGATATGTTAGTGGACCATGAAATGTATTTTGAAAAATGTGCAGACTTTGCCAAATCCTTCCTCCAACTATATATCAAAGAGGATGATGTATTATTCAGAGTGCTCTTGCAATTGCTTTGTGTACCTTGTCCTGCAGAGAAACAGTTTGAAGAGGAAAGGGGATCATTTCAGGATTCAAAGGGCAATGTGCTTTTCTTTGTCTCAGATCTTTTCAATCCTGTACTCTTATTCCATCTATTTCTTGTAGAGTTAAGCTATGACCATCAGGTGCTTCTTGATTACCTTATTTCAAAAGATACTGGAATCAGTTGTGCAGAATATCTTTTAAGGTGCTTGCGTAAAGTGTGTGATTCATGGAGCTTATTTGGTGGTGTGCAGTGCGATATATGGAGTGATATCAGGATGAATTGTGAACAAATTATGCTGCATGTAGTTATTGCAGTAGTGGTATCTTCTACACTAGCTAGCCTTAAATTATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LINES_C LINES_N

Protein Analysis

581

Amino Acids

65.4

Weight (kDa)

5.0

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LINES_N PF14694 428 - 543 7.7e-09 Lines N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1255
AccII CGCG 1 cut(s) 621
AccIII TCCGGA 1 cut(s) 139
AciI CCGC 7 cut(s) 100, 296, 365, 482, 732, 839, 991
AclWI GGATC 3 cut(s) 139, 763, 1432
AcsI RAATTY 2 cut(s) 135, 287
AcuI CTGAAG 1 cut(s) 536
AcyI GRCGYC 1 cut(s) 1252
AfaI GTAC 5 cut(s) 32, 113, 804, 1386, 1485
AfiI CCNNNNNNNGG 1 cut(s) 155
AjnI CCWGG 2 cut(s) 653, 748
AluBI AGCT 7 cut(s) 404, 811, 985, 1231, 1518, 1623, 1727
AluI AGCT 7 cut(s) 404, 811, 985, 1231, 1518, 1623, 1727
Alw21I GWGCWC 1 cut(s) 1368
Alw26I GTCTC 3 cut(s) 118, 286, 1468
AlwI GGATC 3 cut(s) 139, 763, 1432
Aor13HI TCCGGA 1 cut(s) 139
AoxI GGCC 1 cut(s) 258
ApeKI GCWGC 1 cut(s) 1687
ApoI RAATTY 2 cut(s) 135, 287
AspS9I GGNCC 2 cut(s) 626, 1276
AsuHPI GGTGA 2 cut(s) 70, 479
AsuNHI GCTAGC 1 cut(s) 1727
AvaII GGWCC 2 cut(s) 626, 1276
BbsI GAAGAC 3 cut(s) 56, 1080, 1247
Bbv12I GWGCWC 1 cut(s) 1368
BbvI GCAGC 1 cut(s) 1674
BccI CCATC 3 cut(s) 1104, 1503, 1533
BcgI CGANNNNNNTGC 2 cut(s) 599, 633
BciT130I CCWGG 2 cut(s) 655, 750
BclI TGATCA 1 cut(s) 903
BcoDI GTCTC 3 cut(s) 118, 286, 1468
BfaI CTAG 6 cut(s) 405, 420, 689, 761, 1724, 1728
BfmI CTRYAG 2 cut(s) 477, 1395
BglII AGATCT 1 cut(s) 1468
BisI GCNGC 6 cut(s) 101, 297, 366, 483, 992, 1688
BlsI GCNGC 6 cut(s) 102, 298, 367, 484, 993, 1689
Bme1390I CCNGG 2 cut(s) 655, 750
Bme18I GGWCC 2 cut(s) 626, 1276
BmgT120I GGNCC 2 cut(s) 626, 1276
BmrFI CCNGG 2 cut(s) 655, 750
BmrI ACTGGG 1 cut(s) 322
BmsI GCATC 7 cut(s) 84, 262, 307, 628, 733, 778, 873
BmtI GCTAGC 1 cut(s) 1731
BmuI ACTGGG 1 cut(s) 322
BpiI GAAGAC 3 cut(s) 56, 1080, 1247
BpmI CTGGAG 4 cut(s) 420, 637, 732, 1031
Bpu10I CCTNAGC 1 cut(s) 807
BsaAI YACGTR 1 cut(s) 506
BsaHI GRCGYC 1 cut(s) 1252
BsaJI CCNNGG 4 cut(s) 596, 654, 717, 826
BsaWI WCCGGW 2 cut(s) 139, 1055
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 6 cut(s) 317, 403, 476, 776, 1244, 1567
Bse3DI GCAATG 3 cut(s) 190, 271, 1453
BseAI TCCGGA 1 cut(s) 139
BseBI CCWGG 2 cut(s) 655, 750
BseDI CCNNGG 4 cut(s) 596, 654, 717, 826
BseGI GGATG 5 cut(s) 322, 541, 772, 1351, 1671
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 3 cut(s) 190, 271, 1453
BseMII CTCAG 4 cut(s) 185, 522, 1118, 1479
BseNI ACTGG 6 cut(s) 317, 403, 476, 776, 1244, 1567
BseXI GCAGC 1 cut(s) 1674
BseYI CCCAGC 1 cut(s) 15
BsgI GTGCAG 3 cut(s) 1323, 1596, 1658
Bsh1236I CGCG 1 cut(s) 621
BshFI GGCC 1 cut(s) 260
BsiHKAI GWGCWC 1 cut(s) 1368
BsiSI CCGG 2 cut(s) 140, 1056
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 3 cut(s) 118, 286, 1468
BsnI GGCC 1 cut(s) 260
Bsp1286I GDGCHC 1 cut(s) 1368
Bsp13I TCCGGA 1 cut(s) 139
Bsp143I GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
BspACI CCGC 7 cut(s) 100, 296, 365, 482, 732, 839, 991
BspANI GGCC 1 cut(s) 260
BspCNI CTCAG 4 cut(s) 186, 523, 1119, 1478
BspEI TCCGGA 1 cut(s) 139
BspFNI CGCG 1 cut(s) 621
BspMAI CTGCAG 1 cut(s) 1399
BspOI GCTAGC 1 cut(s) 1731
BspPI GGATC 3 cut(s) 139, 763, 1432
BspQI GCTCTTC 1 cut(s) 975
BsrDI GCAATG 3 cut(s) 190, 271, 1453
BsrI ACTGG 6 cut(s) 317, 403, 476, 776, 1244, 1567
BssECI CCNNGG 4 cut(s) 596, 654, 717, 826
BssMI GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
BssNI GRCGYC 1 cut(s) 1252
BssT1I CCWWGG 3 cut(s) 596, 717, 826
Bst2UI CCWGG 2 cut(s) 655, 750
Bst4CI ACNGT 7 cut(s) 30, 116, 232, 277, 592, 1224, 1407
Bst6I CTCTTC 2 cut(s) 975, 1407
BstACI GRCGYC 1 cut(s) 1252
BstAPI GCANNNNNTGC 2 cut(s) 362, 1310
BstBAI YACGTR 1 cut(s) 506
BstC8I GCNNGC 5 cut(s) 73, 262, 837, 1598, 1729
BstDEI CTNAG 6 cut(s) 194, 453, 531, 807, 1127, 1465
BstF5I GGATG 5 cut(s) 322, 541, 772, 1351, 1671
BstFNI CGCG 1 cut(s) 621
BstKTI GATC 6 cut(s) 147, 412, 758, 906, 1427, 1471
BstMAI GTCTC 3 cut(s) 118, 286, 1468
BstMBI GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
BstMWI GCNNNNNNNGC 4 cut(s) 362, 401, 991, 1310
BstNI CCWGG 2 cut(s) 655, 750
BstNSI RCATGY 1 cut(s) 1694
BstSCI CCNGG 2 cut(s) 653, 748
BstSFI CTRYAG 2 cut(s) 477, 1395
BstUI CGCG 1 cut(s) 621
BstV1I GCAGC 1 cut(s) 1674
BstV2I GAAGAC 3 cut(s) 56, 1080, 1247
BstX2I RGATCY 3 cut(s) 144, 755, 1468
BstYI RGATCY 3 cut(s) 144, 755, 1468
BsuRI GGCC 1 cut(s) 260
BtsCI GGATG 5 cut(s) 322, 541, 772, 1351, 1671
BtsI GCAGTG 3 cut(s) 360, 1205, 1646
BtsIMutI CAGTG 7 cut(s) 237, 310, 360, 597, 783, 1205, 1646
Cac8I GCNNGC 5 cut(s) 73, 262, 837, 1598, 1729
Cfr13I GGNCC 2 cut(s) 626, 1276
Csp6I GTAC 5 cut(s) 31, 112, 803, 1385, 1484
CviAII CATG 7 cut(s) 375, 442, 611, 951, 1280, 1617, 1691
CviQI GTAC 5 cut(s) 31, 112, 803, 1385, 1484
DdeI CTNAG 6 cut(s) 194, 453, 531, 807, 1127, 1465
DpnI GATC 6 cut(s) 146, 411, 757, 905, 1426, 1470
DpnII GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
DraI TTTAAA 2 cut(s) 880, 1140
Eam1104I CTCTTC 2 cut(s) 975, 1407
EarI CTCTTC 2 cut(s) 975, 1407
Eco130I CCWWGG 3 cut(s) 596, 717, 826
Eco32I GATATC 1 cut(s) 1660
Eco47I GGWCC 2 cut(s) 626, 1276
Eco57I CTGAAG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 287
EcoRII CCWGG 2 cut(s) 653, 748
EcoRV GATATC 1 cut(s) 1660
EcoT14I CCWWGG 3 cut(s) 596, 717, 826
ErhI CCWWGG 3 cut(s) 596, 717, 826
FaeI CATG 7 cut(s) 378, 445, 614, 954, 1283, 1620, 1694
FalI AAGNNNNNCTT 2 cut(s) 1239, 1271
FatI CATG 7 cut(s) 374, 441, 610, 950, 1279, 1616, 1690
FbaI TGATCA 1 cut(s) 903
Fnu4HI GCNGC 6 cut(s) 101, 297, 366, 483, 992, 1688
FokI GGATG 5 cut(s) 329, 548, 759, 1358, 1678
Fsp4HI GCNGC 6 cut(s) 101, 297, 366, 483, 992, 1688
FspBI CTAG 6 cut(s) 405, 420, 689, 761, 1724, 1728
GluI GCNGC 6 cut(s) 101, 297, 366, 483, 992, 1688
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 4 cut(s) 420, 637, 732, 1031
HaeIII GGCC 1 cut(s) 260
HapII CCGG 2 cut(s) 140, 1056
Hin1I GRCGYC 1 cut(s) 1252
Hin1II CATG 7 cut(s) 378, 445, 614, 954, 1283, 1620, 1694
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 809
HpaII CCGG 2 cut(s) 140, 1056
HphI GGTGA 2 cut(s) 70, 479
Hpy166II GTNNAC 4 cut(s) 91, 1276, 1385, 1676
Hpy8I GTNNAC 4 cut(s) 91, 1276, 1385, 1676
HpyAV CCTTC 3 cut(s) 1031, 1211, 1330
HpyCH4III ACNGT 7 cut(s) 30, 116, 232, 277, 592, 1224, 1407
HpyCH4IV ACGT 3 cut(s) 505, 817, 1252
HpyF10VI GCNNNNNNNGC 4 cut(s) 362, 401, 991, 1310
HpyF3I CTNAG 6 cut(s) 194, 453, 531, 807, 1127, 1465
HpySE526I ACGT 3 cut(s) 505, 817, 1252
Hsp92I GRCGYC 1 cut(s) 1252
Hsp92II CATG 7 cut(s) 378, 445, 614, 954, 1283, 1620, 1694
Kpn2I TCCGGA 1 cut(s) 139
Ksp22I TGATCA 1 cut(s) 903
Kzo9I GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
LguI GCTCTTC 1 cut(s) 975
LmnI GCTCC 6 cut(s) 401, 656, 751, 832, 1228, 1620
Lsp1109I GCAGC 1 cut(s) 1674
LweI GCATC 7 cut(s) 84, 262, 307, 628, 733, 778, 873
MaeI CTAG 6 cut(s) 405, 420, 689, 761, 1724, 1728
MaeII ACGT 3 cut(s) 505, 817, 1252
MaeIII GTNAC 1 cut(s) 467
MalI GATC 6 cut(s) 146, 411, 757, 905, 1426, 1470
MboI GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
MboII GAAGA 8 cut(s) 56, 992, 1037, 1080, 1221, 1247, 1424, 1707
MfeI CAATTG 1 cut(s) 1373
MflI RGATCY 3 cut(s) 144, 755, 1468
MhlI GDGCHC 1 cut(s) 1368
MlyI GAGTC 3 cut(s) 120, 247, 1175
MmeI TCCRAC 6 cut(s) 148, 653, 1140, 1206, 1283, 1351
MnlI CCTC 7 cut(s) 526, 559, 946, 1122, 1334, 1336, 1408
MroI TCCGGA 1 cut(s) 139
MseI TTAA 9 cut(s) 206, 434, 573, 879, 1139, 1233, 1514, 1589, 1734
MslI CAYNNNNRTG 1 cut(s) 315
MspI CCGG 2 cut(s) 140, 1056
MspR9I CCNGG 2 cut(s) 655, 750
MunI CAATTG 1 cut(s) 1373
MvaI CCWGG 2 cut(s) 655, 750
MvnI CGCG 1 cut(s) 621
MwoI GCNNNNNNNGC 4 cut(s) 362, 401, 991, 1310
NdeII GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
NheI GCTAGC 1 cut(s) 1727
NlaIII CATG 7 cut(s) 378, 445, 614, 954, 1283, 1620, 1694
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 1 cut(s) 1694
PciSI GCTCTTC 1 cut(s) 975
PcsI WCGNNNNNNNCGW 1 cut(s) 502
PfeI GAWTC 8 cut(s) 616, 700, 789, 932, 1067, 1436, 1566, 1613
PkrI GCNGC 6 cut(s) 102, 298, 367, 484, 993, 1689
PleI GAGTC 3 cut(s) 120, 246, 1175
PpsI GAGTC 3 cut(s) 120, 246, 1175
Ppu21I YACGTR 1 cut(s) 506
Psp6I CCWGG 2 cut(s) 653, 748
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 2 cut(s) 653, 748
PspPI GGNCC 2 cut(s) 626, 1276
PstI CTGCAG 1 cut(s) 1399
PsuI RGATCY 3 cut(s) 144, 755, 1468
RsaI GTAC 5 cut(s) 32, 113, 804, 1386, 1485
RsaNI GTAC 5 cut(s) 31, 112, 803, 1385, 1484
RseI CAYNNNNRTG 1 cut(s) 315
SapI GCTCTTC 1 cut(s) 975
SaqAI TTAA 9 cut(s) 206, 434, 573, 879, 1139, 1233, 1514, 1589, 1734
SatI GCNGC 6 cut(s) 101, 297, 366, 483, 992, 1688
Sau3AI GATC 6 cut(s) 144, 409, 755, 903, 1424, 1468
Sau96I GGNCC 2 cut(s) 626, 1276
SchI GAGTC 3 cut(s) 120, 247, 1175
ScrFI CCNGG 2 cut(s) 655, 750
SduI GDGCHC 1 cut(s) 1368
SfaNI GCATC 7 cut(s) 84, 262, 307, 628, 733, 778, 873
SfcI CTRYAG 2 cut(s) 477, 1395
SinI GGWCC 2 cut(s) 626, 1276
SmiMI CAYNNNNRTG 1 cut(s) 315
SsiI CCGC 7 cut(s) 100, 296, 365, 482, 732, 839, 991
SspMI CTAG 6 cut(s) 405, 420, 689, 761, 1724, 1728
StyD4I CCNGG 2 cut(s) 653, 748
StyI CCWWGG 3 cut(s) 596, 717, 826
TaaI ACNGT 7 cut(s) 30, 116, 232, 277, 592, 1224, 1407
TaiI ACGT 3 cut(s) 508, 820, 1255
TaqI TCGA 3 cut(s) 386, 723, 741
TatI WGTACW 1 cut(s) 1483
TauI GCSGC 5 cut(s) 103, 299, 368, 485, 994
TfiI GAWTC 8 cut(s) 616, 700, 789, 932, 1067, 1436, 1566, 1613
Tru1I TTAA 9 cut(s) 206, 434, 573, 879, 1139, 1233, 1514, 1589, 1734
Tru9I TTAA 9 cut(s) 206, 434, 573, 879, 1139, 1233, 1514, 1589, 1734
TscAI CASTG 7 cut(s) 237, 317, 367, 597, 783, 1205, 1646
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 1 cut(s) 1687
Tsp45I GTSAC 1 cut(s) 467
TspGWI ACGGA 1 cut(s) 171
TspRI CASTG 7 cut(s) 237, 317, 367, 597, 783, 1205, 1646
VpaK11BI GGWCC 2 cut(s) 626, 1276
XapI RAATTY 2 cut(s) 135, 287
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 1 cut(s) 1694
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XspI CTAG 6 cut(s) 405, 420, 689, 761, 1724, 1728
ZraI GACGTC 1 cut(s) 1253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.