RchiOBHm_Chr1g0329901

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005021
Physical Location & Seq
Forward (+)
19606467 .. 19610509
4043 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55916

Sequence Viewer

Length: 1995 bp
ATGTGCAGCGATTCAAATCTGACGAAGCTTCACCGTCTGATCGACGATTCTCTCCGTCCGGTTTTCAATCTCGGGCTTTTGCAGGAGCCAGATGTCGTTTCGGTAACGAAAGAGAGAGAAATTCTGGTCAGTCTCGCTCAGATTCTTCGGCAAGTGAAACTCTGGATTGGAGAAGTTGATTCCCATTCTGATAATGGAGTGTTGATTGGATTGGATTTTGAGAATGAGTGTATGTGTCTGAGTAAAATCTTAACTCACTTGATTGTTTTCCTAACAGTGGAGAGTCAATATGTGAAGCATCTGGCCTGCAATGTTCTAGCTGCTGTCTCCGAATTCGTGGCGGCTTCGGGAAGCCACTGGGATGCCTTTATTCGTTTGTTGTGTGATTGTATGGATTTGGCAATCACTGCGGCAGTTTCATGTTCTACATCGACTTTGGCAACTGGAGCTAGTGATCTTTGTTCTAGTTCATTGAGTTTAATTCTCGTGCTGAAACCTAAGGTGAAAAATGGTGACTGGTCTGTAGCGGCTGGTGTTGTTCGGGTGCTACGTGATATACTGAAGTATTTGAAGTCTGAGGATGATGACGAACTTGTTGAAGTGTTCATTGAGGCTGTTAATTCTTTTCTTTCAACAGTGCCTTGGGATTTATTGCATGAGATTCGTGTTGGTCCAAATGCTGATGCTCTGAAATGCTCCAGGGCAGATGTTTCATTCCAAAGAACCTTGTTTCTAGGAAACTTGATTCAGTTTCTCTGTTCCTTGGTCGAGCAAGGCAGTGTTGTCGATGCTCCAGGTGGATCTCTAGACCAGCATCATCCAGTGTTTTCTAGAATCATCAACCTTGTACCTAAGCTTTTACGTTTATGCCTTGGGGAGCAGGCGGATTGTGTTATCTCTCACAACTGCATCTCTCAATACTTTAAACACAAGTTATTGGTGCTAATGATCAGGCTCATTGTCCAAACTTGTCCAGAATCCTCAATTCTTGTTTCATGGTTGCAACTTATCCATTATTACTTTGAAGAGCTTTTGCGGCAACCCATTTCTACTCTGGAGTGTGATCAGGAAGATTGTTTGGAAGGCTCTCCATTTCTATCCAGTGTTTCTGATTCAGAAGTAAATAGTCTGTCTTCTCGCCATCTGCAAAGACAGGCTGTGTTCCTTTTTCTGAGGTGTTCTTTTAGTTTGATTAACTCAAATGGAGGCACTAACAGAAAATGTGCTTGTGCAAGTTGGAATTTATGCTTGGACTATGACTCAAATGCAGAGTTGCAGTGTTGTGGAAGAAAGGAAGGACTGTTGGAGCTTAATAACTGGCTTCAAGGACATCTTCCAACTGATATGTTTGTGGACCATGAAATGTATTTTGAAAAATGTGCAGACTTTGCCAAATCCTTCCTCCAACTATATATCAAAGAGGATGATGTATTATTCAGAGTGCTCTTGCAATTGCTTTGTGTACCTTGTCCTGCAGAGAAACAGTTTGAAGAGGAAAGGGGATCATTTCAGGATTCAAAGGGCAATGTGCTTTTCTTTGTTTCAGATCTTTTCAATCCTGTACTCTTATTCCATCTATTTCTTGTAGAGTTAAGCTATGACCATCAGGTGCTTCTTGATTACCGTATTACAAAAGATACTGGAATCAGTTGTGCAGAATATCTTTTAAGGTGCTTGCGTAAAGTGTGTGATTCATGGAGCTTATTTGTGGAATTTCCACCGAGTGGACAAGCTATTAATCGATCATCTTGCAAGAAAAGAAAAGTTTCATTGAATGGGTCGAGTTTCTGTGGAGCAGATTTGTTTGCGCTAGTGAAAGACTCTGGAGGTTTGTTTCTTGAAGATGAATGTGACGATGAAAAGAAATATGATTTCAAGCACACTCGAGAGAATTTCCAAAAAGCCAAAGAGTGCTTAATTTCGTTGAAAAATTCGATTGCTCTTTTGAACCGCTTAATGAGGTTTCAGGAACTCTGTTTCGAGGAGGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LINES_C LINES_N

Protein Analysis

664

Amino Acids

74.91

Weight (kDa)

5.16

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LINES_N PF14694 448 - 571 5.2e-08 Lines N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1724
AciI CCGC 6 cut(s) 341, 410, 527, 884, 1036, 1951
AclWI GGATC 2 cut(s) 808, 1510
AcsI RAATTY 6 cut(s) 120, 332, 1240, 1712, 1891, 1930
AcuI CTGAAG 1 cut(s) 581
AdeI CACNNNGTG 1 cut(s) 1724
AfaI GTAC 3 cut(s) 849, 1464, 1563
AfiI CCNNNNNNNGG 2 cut(s) 277, 1724
AjnI CCWGG 2 cut(s) 698, 793
AjuI GAANNNNNNNTTGG 2 cut(s) 1232, 1264
AluBI AGCT 9 cut(s) 28, 320, 449, 856, 1030, 1309, 1596, 1701, 1733
AluI AGCT 9 cut(s) 28, 320, 449, 856, 1030, 1309, 1596, 1701, 1733
Alw21I GWGCWC 1 cut(s) 1446
Alw26I GTCTC 2 cut(s) 137, 331
AlwI GGATC 2 cut(s) 808, 1510
Ama87I CYCGRG 2 cut(s) 71, 1884
AoxI GGCC 1 cut(s) 303
ApeKI GCWGC 2 cut(s) 6, 320
ApoI RAATTY 6 cut(s) 120, 332, 1240, 1712, 1891, 1930
AseI ATTAAT 1 cut(s) 1737
Asp700I GAANNNNTTC 1 cut(s) 1765
AspLEI GCGC 1 cut(s) 1810
AspS9I GGNCC 2 cut(s) 671, 1354
AsuHPI GGTGA 3 cut(s) 23, 514, 524
AvaI CYCGRG 2 cut(s) 71, 1884
AvaII GGWCC 2 cut(s) 671, 1354
AxyI CCTNAGG 1 cut(s) 498
BauI CACGAG 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 1125
Bbv12I GWGCWC 1 cut(s) 1446
BbvI GCAGC 2 cut(s) 18, 307
BccI CCATC 3 cut(s) 1149, 1581, 1611
BcgI CGANNNNNNTGC 6 cut(s) 644, 678, 766, 800, 1731, 1765
BciT130I CCWGG 2 cut(s) 700, 795
BclI TGATCA 2 cut(s) 948, 1063
BcoDI GTCTC 2 cut(s) 137, 331
BfaI CTAG 7 cut(s) 317, 450, 465, 734, 806, 831, 1811
BfmI CTRYAG 2 cut(s) 522, 1473
BglII AGATCT 1 cut(s) 1546
BisI GCNGC 6 cut(s) 7, 321, 342, 411, 528, 1037
BlsI GCNGC 6 cut(s) 8, 322, 343, 412, 529, 1038
Bme1390I CCNGG 2 cut(s) 700, 795
Bme18I GGWCC 2 cut(s) 671, 1354
BmeT110I CYCGRG 2 cut(s) 71, 1884
BmgT120I GGNCC 2 cut(s) 671, 1354
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 700, 795
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 6 cut(s) 307, 352, 673, 778, 823, 918
BmuI ACTGGG 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 1125
BpmI CTGGAG 5 cut(s) 465, 682, 777, 1076, 1845
Bpu10I CCTNAGC 1 cut(s) 852
Bsa29I ATCGAT 1 cut(s) 1741
BsaAI YACGTR 1 cut(s) 551
BsaBI GATNNNNATC 1 cut(s) 15
BsaJI CCNNGG 4 cut(s) 641, 699, 762, 871
BsaWI WCCGGW 1 cut(s) 58
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 1724
Bse1I ACTGG 7 cut(s) 362, 448, 521, 821, 1101, 1322, 1645
Bse21I CCTNAGG 1 cut(s) 498
Bse3DI GCAATG 2 cut(s) 316, 1531
Bse8I GATNNNNATC 1 cut(s) 15
BseBI CCWGG 2 cut(s) 700, 795
BseCI ATCGAT 1 cut(s) 1741
BseDI CCNNGG 4 cut(s) 641, 699, 762, 871
BseGI GGATG 4 cut(s) 367, 586, 817, 1429
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 2 cut(s) 277, 1724
BseMI GCAATG 2 cut(s) 316, 1531
BseMII CTCAG 4 cut(s) 152, 230, 567, 1163
BseNI ACTGG 7 cut(s) 362, 448, 521, 821, 1101, 1322, 1645
BseXI GCAGC 2 cut(s) 18, 307
BsgI GTGCAG 3 cut(s) 25, 1401, 1674
BshFI GGCC 1 cut(s) 305
BshVI ATCGAT 1 cut(s) 1741
BsiHKAI GWGCWC 1 cut(s) 1446
BsiHKCI CYCGRG 2 cut(s) 71, 1884
BsiSI CCGG 1 cut(s) 59
BslI CCNNNNNNNGG 2 cut(s) 277, 1724
BsmAI GTCTC 2 cut(s) 137, 331
BsnI GGCC 1 cut(s) 305
BsoBI CYCGRG 2 cut(s) 71, 1884
Bsp1286I GDGCHC 1 cut(s) 1446
Bsp143I GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
BspACI CCGC 6 cut(s) 341, 410, 527, 884, 1036, 1951
BspANI GGCC 1 cut(s) 305
BspCNI CTCAG 4 cut(s) 151, 231, 568, 1164
BspDI ATCGAT 1 cut(s) 1741
BspLI GGNNCC 1 cut(s) 87
BspMAI CTGCAG 1 cut(s) 1477
BspPI GGATC 2 cut(s) 808, 1510
BspQI GCTCTTC 1 cut(s) 1020
BsrDI GCAATG 2 cut(s) 316, 1531
BsrI ACTGG 7 cut(s) 362, 448, 521, 821, 1101, 1322, 1645
BssECI CCNNGG 4 cut(s) 641, 699, 762, 871
BssMI GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
BssSI CACGAG 1 cut(s) 485
BssT1I CCWWGG 3 cut(s) 641, 762, 871
Bst2BI CACGAG 1 cut(s) 485
Bst2UI CCWGG 2 cut(s) 700, 795
Bst4CI ACNGT 6 cut(s) 35, 277, 637, 1302, 1485, 1625
Bst6I CTCTTC 2 cut(s) 1020, 1485
BstAPI GCANNNNNTGC 2 cut(s) 407, 1388
BstBAI YACGTR 1 cut(s) 551
BstC8I GCNNGC 3 cut(s) 307, 882, 1676
BstDEI CTNAG 6 cut(s) 138, 239, 498, 576, 852, 1172
BstF5I GGATG 4 cut(s) 367, 586, 817, 1429
BstHHI GCGC 1 cut(s) 1810
BstKTI GATC 8 cut(s) 42, 457, 803, 951, 1066, 1505, 1549, 1745
BstMAI GTCTC 2 cut(s) 137, 331
BstMBI GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
BstMWI GCNNNNNNNGC 4 cut(s) 407, 446, 1036, 1388
BstNI CCWGG 2 cut(s) 700, 795
BstSCI CCNGG 2 cut(s) 698, 793
BstSFI CTRYAG 2 cut(s) 522, 1473
BstV1I GCAGC 2 cut(s) 18, 307
BstV2I GAAGAC 1 cut(s) 1125
BstX2I RGATCY 2 cut(s) 800, 1546
BstYI RGATCY 2 cut(s) 800, 1546
Bsu15I ATCGAT 1 cut(s) 1741
Bsu36I CCTNAGG 1 cut(s) 498
BsuRI GGCC 1 cut(s) 305
BsuTUI ATCGAT 1 cut(s) 1741
BtsCI GGATG 4 cut(s) 367, 586, 817, 1429
BtsI GCAGTG 3 cut(s) 405, 784, 1283
BtsIMutI CAGTG 8 cut(s) 282, 355, 405, 642, 784, 828, 1108, 1283
Cac8I GCNNGC 3 cut(s) 307, 882, 1676
CfoI GCGC 1 cut(s) 1810
Cfr13I GGNCC 2 cut(s) 671, 1354
ClaI ATCGAT 1 cut(s) 1741
Csp6I GTAC 3 cut(s) 848, 1463, 1562
CviAII CATG 5 cut(s) 420, 656, 996, 1358, 1695
CviQI GTAC 3 cut(s) 848, 1463, 1562
DdeI CTNAG 6 cut(s) 138, 239, 498, 576, 852, 1172
DpnI GATC 8 cut(s) 41, 456, 802, 950, 1065, 1504, 1548, 1744
DpnII GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
DraI TTTAAA 1 cut(s) 925
DraIII CACNNNGTG 1 cut(s) 1724
Eam1104I CTCTTC 2 cut(s) 1020, 1485
EarI CTCTTC 2 cut(s) 1020, 1485
EciI GGCGGA 1 cut(s) 899
Eco130I CCWWGG 3 cut(s) 641, 762, 871
Eco47I GGWCC 2 cut(s) 671, 1354
Eco57I CTGAAG 1 cut(s) 581
Eco81I CCTNAGG 1 cut(s) 498
Eco88I CYCGRG 2 cut(s) 71, 1884
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 698, 793
EcoT14I CCWWGG 3 cut(s) 641, 762, 871
ErhI CCWWGG 3 cut(s) 641, 762, 871
FaeI CATG 5 cut(s) 423, 659, 999, 1361, 1698
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FatI CATG 5 cut(s) 419, 655, 995, 1357, 1694
FbaI TGATCA 2 cut(s) 948, 1063
Fnu4HI GCNGC 6 cut(s) 7, 321, 342, 411, 528, 1037
FokI GGATG 4 cut(s) 374, 593, 804, 1436
Fsp4HI GCNGC 6 cut(s) 7, 321, 342, 411, 528, 1037
FspBI CTAG 7 cut(s) 317, 450, 465, 734, 806, 831, 1811
GlaI GCGC 1 cut(s) 1809
GluI GCNGC 6 cut(s) 7, 321, 342, 411, 528, 1037
GsuI CTGGAG 5 cut(s) 465, 682, 777, 1076, 1845
HaeIII GGCC 1 cut(s) 305
HapII CCGG 1 cut(s) 59
HhaI GCGC 1 cut(s) 1810
Hin1II CATG 5 cut(s) 423, 659, 999, 1361, 1698
Hin6I GCGC 1 cut(s) 1808
HinP1I GCGC 1 cut(s) 1808
HindIII AAGCTT 2 cut(s) 26, 854
HpaII CCGG 1 cut(s) 59
HphI GGTGA 3 cut(s) 23, 514, 524
Hpy166II GTNNAC 3 cut(s) 1354, 1463, 1727
Hpy8I GTNNAC 3 cut(s) 1354, 1463, 1727
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 3 cut(s) 1076, 1289, 1408
HpyCH4III ACNGT 6 cut(s) 35, 277, 637, 1302, 1485, 1625
HpyCH4IV ACGT 2 cut(s) 550, 862
HpyF10VI GCNNNNNNNGC 4 cut(s) 407, 446, 1036, 1388
HpyF3I CTNAG 6 cut(s) 138, 239, 498, 576, 852, 1172
HpySE526I ACGT 2 cut(s) 550, 862
Hsp92II CATG 5 cut(s) 423, 659, 999, 1361, 1698
HspAI GCGC 1 cut(s) 1808
Ksp22I TGATCA 2 cut(s) 948, 1063
Kzo9I GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
LguI GCTCTTC 1 cut(s) 1020
LmnI GCTCC 8 cut(s) 85, 446, 701, 796, 877, 1306, 1698, 1793
Lsp1109I GCAGC 2 cut(s) 18, 307
LweI GCATC 6 cut(s) 307, 352, 673, 778, 823, 918
MaeI CTAG 7 cut(s) 317, 450, 465, 734, 806, 831, 1811
MaeII ACGT 2 cut(s) 550, 862
MaeIII GTNAC 3 cut(s) 103, 512, 1850
MalI GATC 8 cut(s) 41, 456, 802, 950, 1065, 1504, 1548, 1744
MboI GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
MboII GAAGA 8 cut(s) 137, 1037, 1082, 1125, 1299, 1325, 1502, 1853
MfeI CAATTG 1 cut(s) 1451
MflI RGATCY 2 cut(s) 800, 1546
MhlI GDGCHC 1 cut(s) 1446
MlyI GAGTC 3 cut(s) 292, 1253, 1814
MmeI TCCRAC 4 cut(s) 1217, 1284, 1361, 1429
MroXI GAANNNNTTC 1 cut(s) 1765
MslI CAYNNNNRTG 1 cut(s) 360
MspI CCGG 1 cut(s) 59
MspR9I CCNGG 2 cut(s) 700, 795
MunI CAATTG 1 cut(s) 1451
MvaI CCWGG 2 cut(s) 700, 795
MwoI GCNNNNNNNGC 4 cut(s) 407, 446, 1036, 1388
NdeII GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
NlaIII CATG 5 cut(s) 423, 659, 999, 1361, 1698
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 512, 1850
PaeR7I CTCGAG 1 cut(s) 1884
PciSI GCTCTTC 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 1765
PflMI CCANNNNNTGG 1 cut(s) 1724
PkrI GCNGC 6 cut(s) 8, 322, 343, 412, 529, 1038
PleI GAGTC 3 cut(s) 291, 1253, 1814
PpsI GAGTC 3 cut(s) 291, 1253, 1814
Ppu21I YACGTR 1 cut(s) 551
PshBI ATTAAT 1 cut(s) 1737
Psp6I CCWGG 2 cut(s) 698, 793
PspGI CCWGG 2 cut(s) 698, 793
PspN4I GGNNCC 1 cut(s) 87
PspPI GGNCC 2 cut(s) 671, 1354
PstI CTGCAG 1 cut(s) 1477
PsuI RGATCY 2 cut(s) 800, 1546
RsaI GTAC 3 cut(s) 849, 1464, 1563
RsaNI GTAC 3 cut(s) 848, 1463, 1562
RseI CAYNNNNRTG 1 cut(s) 360
SapI GCTCTTC 1 cut(s) 1020
SatI GCNGC 6 cut(s) 7, 321, 342, 411, 528, 1037
Sau3AI GATC 8 cut(s) 39, 454, 800, 948, 1063, 1502, 1546, 1742
Sau96I GGNCC 2 cut(s) 671, 1354
SchI GAGTC 3 cut(s) 292, 1253, 1814
ScrFI CCNGG 2 cut(s) 700, 795
SduI GDGCHC 1 cut(s) 1446
SfaNI GCATC 6 cut(s) 307, 352, 673, 778, 823, 918
SfcI CTRYAG 2 cut(s) 522, 1473
Sfr274I CTCGAG 1 cut(s) 1884
SinI GGWCC 2 cut(s) 671, 1354
SlaI CTCGAG 1 cut(s) 1884
SmiMI CAYNNNNRTG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 1884
SmoI CTYRAG 1 cut(s) 1884
SsiI CCGC 6 cut(s) 341, 410, 527, 884, 1036, 1951
SspMI CTAG 7 cut(s) 317, 450, 465, 734, 806, 831, 1811
StyD4I CCNGG 2 cut(s) 698, 793
StyI CCWWGG 3 cut(s) 641, 762, 871
TaaI ACNGT 6 cut(s) 35, 277, 637, 1302, 1485, 1625
TaiI ACGT 2 cut(s) 553, 865
TaqI TCGA 9 cut(s) 42, 431, 768, 786, 1741, 1781, 1885, 1934, 1980
TatI WGTACW 1 cut(s) 1561
TauI GCSGC 4 cut(s) 344, 413, 530, 1039
TscAI CASTG 8 cut(s) 282, 362, 412, 642, 784, 828, 1108, 1283
TseFI GTSAC 2 cut(s) 512, 1850
TseI GCWGC 2 cut(s) 6, 320
Tsp45I GTSAC 2 cut(s) 512, 1850
TspGWI ACGGA 1 cut(s) 44
TspRI CASTG 8 cut(s) 282, 362, 412, 642, 784, 828, 1108, 1283
Van91I CCANNNNNTGG 1 cut(s) 1724
VpaK11BI GGWCC 2 cut(s) 671, 1354
VspI ATTAAT 1 cut(s) 1737
XapI RAATTY 6 cut(s) 120, 332, 1240, 1712, 1891, 1930
XbaI TCTAGA 2 cut(s) 805, 830
XcmI CCANNNNNNNNNTGG 2 cut(s) 191, 1051
XhoI CTCGAG 1 cut(s) 1884
XmnI GAANNNNTTC 1 cut(s) 1765
XspI CTAG 7 cut(s) 317, 450, 465, 734, 806, 831, 1811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.